Journal of the Taiwan Institute of Chemical Engineers
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2018
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IF 3.849
Tao Li,Shaoyu Lü,Shaofei Zhang,Chunmei Gao,Mingzhu Liu
Excessive application of fertilizers and their losses have exerted a great threat on soil environment. Slow- or controlled-release fertilizers have been widely produced to alleviate the problem. However, most of them can not remedy contaminated soil environment, and even make it worse. To improve nutrient use efficiency and reduce soil environmental pollution, lignin, an extensive biomass source, was used to synthesize a lignin-based superabsorbent (LPA), which was then introduced into the fertilizer as an outer coating material to obtain lignin-based multifunctional fertilizer (LMF). Results showed that the introduction of LPA can improve water-holding and water-retention capacity of soil and nutrients release behavior of fertilizers. The cumulative release rates of nitrogen, phosphorus and zinc were 79.5%, 46.7% and 60.1% after 25 days, respectively. Additionally, LMF can efficiently immobilize and restrict the migration of bivalent lead (Pb(II)) by complexation with lignin. About 91.7% of Pb(II) was immobilized after soil-sand mixture with Pb(NO 3 ) 2 was treated by LMF. The pot experiments indicated that the LMF exhibited a positive effect on the growing of corn in Pb(II)-contaminated soil. Therefore, this work provides a promising fertilizer to improve the utilization efficiency of water, sustained release of nutrients, and immobilization of Pb (II) in contaminated soil.
研究领域:
环境科学
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农业科学
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DOI
高影响因子
NANOTECHNOLOGY
·
2015
·Vol. 26
·期 27
·274005
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IF 3.821
Jie Chao,Min Zou,Chi Zhang,Haofan Sun,Dun Pan,Hao Pei,Shao Su,Lihui Yuwen,Chunhai Fan,Lianhui Wang
A novel hydrogen peroxide (H2O2) and nitric oxide (NO) biosensor was fabricated by immobilizing hemoglobin (Hb) on a gold nanoparticle-decorated MoS2 nanosheet (AuNPs@MoS2) nanocomposite film modified glass carbon electrode. The AuNPs@MoS2 nanocomposite not only made the immobilized Hb keep its native biological activity but also facilitated the electron transfer between electrode and the electroactive center of Hb due to its excellent conductivity and biocompatibility. The direct electrochemistry and bioelectrocatalytic activity of Hb were investigated by cyclic voltammetry (CV). The modified electrode showed good electrocatalytic ability toward the reduction of H2O2 and NO. Under optimal conditions, the current response was linear with the concentration of H2O2 and NO in the range from 10 to 300 μM and 10 to 1100 μM with a detection limit of 4 and 5 μM, respectively. This MoS2-based biosensor was sensitive, reproducible and stable, indicating that AuNPs@MoS2 nanocomposite maybe a promising platform to construct electrochemical sensors for chemical and biological molecules detection.
研究领域:
生物传感器
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电化学分析
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材料科学
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DOI
PubMed
高影响因子
RSC Advances
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2016
·Vol. 6
·期 25
·20867-20875
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IF 3.289
Yulan Tang,Sufang Liu,Rongbiao Pi,Zhiyi Cheng
A bi-enzyme immobilized microfluidic device was developed for the rapid enzyme inhibition assay by fluorescence detection. The micro-channels of the chip were activated by using an acidic hydrogen peroxide (H2O2) solution, followed by amination with a 3-aminopropyltriethoxysilane/methyltriethoxysilane (APTES/MTES) mixture, and then followed by glutaraldehyde grafting and subsequent covalent protein binding. The analysis was based on the oxidation of L-tyrosine to its tyrosine dimer in the presence of horseradish peroxidase (HRP) and H2O2, which has maximal excitation and emission wavelengths at 320 nm and 416 nm, respectively. H2O2 was an end product under the catalysis of acetylcholinesterase (AChE) and choline oxidase (ChO) immobilized on the inner surface of a microchip, using acetylcholine (ACh) as the enzyme substrate. Fluorescence of the dimer was directly proportional to the concentration of the H2O2 and partly reflected enzyme activity. Thus, AChE inhibition activity was indicated by a decrease in fluorescence signal detected by a microplate reader. The effects of flow velocities, reaction time and buffer systems (pH, types and concentration) on the reaction system were investigated. The utility of the developed microchip was demonstrated for AChE inhibition studies using donepezil as a model compound, which relied upon the generation of a uniform concentration of substrate and a microfluidically created concentration gradient of inhibitor using a single initial inhibitor concentration. The IC50 value of donepezil was determined and found to correlate well with that obtained in the microplate. Accordingly, the microchip can be applied to the screening of enzyme inhibitor and enzymatic reaction kinetics study, which may be useful for drug discovery and screening.
研究领域:
生物化学
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药物开发
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分析化学
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DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
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2016
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IF 2.624
Jian Qin Zhou,Ting He,Jian Wen Wang
Microbial transglutaminase (mTG) is widely utilized in the PEGylation of pharmaceutical proteins. mTG immobilization can be achieved via covalent bonding on solid supports. However, the catalytic efficiency of mTG immobilized on solid supports was significantly reduced by mass transfer limitation. To overcome this limitation, mTG was covalently immobilized on the thermo-responsive carboxylated poly( N -isopropylacrylamide) (pNIPAM). The pNIPAM-mTG conjugate exhibited reversibly solubility in aqueous solution with a low critical solution temperature (LCST) at 39 °C, i.e., it was insoluble above 39 °C and soluble below 39 °C. The pH dependence of the pNIPAM-mTG conjugate was similar with that of the native mTG. Upon conjugation to pNIPAM, the optimal temperature of mTG shifted down from 50–55 °C to 40–45 °C, and the thermal stability of the conjugate was elevated. The easy separation of the pNIPAM-mTG conjugate with its substrate and the catalytic efficiency of the pNIPAM-mTG conjugate were demonstrated by employing the pNIPAM-mTG conjugate to cross-link bovine serum albumin (BSA) and catalyze PEGylation of therapeutic protein, cytochrome c (Cyt C), respectively. The thermo-responsive mTG is suitable to modify proteins in food processing and biomedical engineering.
研究领域:
生物技术
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生物化学
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食品加工
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生物医学工程
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DOI
PubMed
高影响因子
DALTON TRANSACTIONS
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2017
·Vol. 46
·期 7
·2114-2121
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IF 4.029
Gen Liu,Yan Xu,Yide Han,Junbiao Wu,Junli Xu,Hao Meng,Xia Zhang
A hierarchical zeolitic imidazolate framework-8 (micro/meso-ZIF-8) was fabricated by using cetyltrimethylammonium bromide as a structure-controlling agent and L-histidine as co-templates. Compared to the conventional microporous ZIF-8 (micro-ZIF-8), the hierarchical porous structure of micro/meso-ZIF-8 contains micropores and maximum mesopores of around 35.6 nm. The as-prepared hierarchical micro/meso-ZIF-8 featured a large surface area and superior spontaneous adsorption activity than micro-ZIF-8 towards lysozyme (LZM), bovine hemoglobin (BHb) and bovine serum albumin (BSA), and the adsorption capacity increased with the decreasing of the protein size due to the molecule cutoff effects. The maximum adsorption capacity of LZM on micro/meso-ZIF-8 was higher than most of the reported results under similar adsorption conditions. The analyses of adsorption kinetics and thermodynamics implied that the adsorption mechanism mainly involved physical adsorption. Moreover, the micro/meso-ZIF-8 showed good thermal stability against temperature and excellent regeneration ability in the recycling adsorption experiments. This work proposed herein opens a broad application prospect of hierarchical MOFs in biological molecule separation, immobilization and enrichment.
研究领域:
生物化学
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材料科学
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纳米技术
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2017
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IF 6.216
Yue Sun,Zimo Lou,Jianbing Yu,Xiaoxin Zhou,Dan Lv,Jiasheng Zhou,Shams Ali Baig,Xinhua Xu
Iron sulfide (FeS) nanoparticles were known for their excellent ability to scavenge aqueous Hg(II). However, the aggregation and oxidizability of FeS nanoparticles greatly restricted their practical application. This study developed an Al 2 O 3 -supported nanoscale FeS (FeS/Al 2 O 3 ) which effectively optimized the material performance. Characteristic data suggested that FeS/Al 2 O 3 (mass ratio of FeS to FeS/Al 2 O 3 is 30%) with a large specific surface area (142.7 m 2 g −1 ) could evenly distribute FeS, suppressing the aggregation of FeS successfully. The maximum enrichment capacity of Hg(II) on FeS/Al 2 O 3 achieved ∼313 mg/g at pH 6 and 30 °C. The pH studies indicated that alkaline environment strongly inhibited mercury uptake, while removal rate of 1 mg/L Hg(II) maintained a high level (>97.5%) over the pH range of 3–9. However, FeS/Al 2 O 3 loss was observed at low pH due to the acid corrosion. Additionally, the rates of Hg(II) reduction were almost unaffected over the range of 0.1–100 mM NaCl, but greatly inhibited by the presence of humid acid (HA). The synthetic FeS/Al 2 O 3 remained high mercury removal efficiency (over 95% in 1 mg/L Hg(II)) and low iron dissolution rate when preserved for 30 days, showing good long-term stability and remarkable application potential.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
Catalysts
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2017
·Vol. 7
·期 11
·327
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IF 3.082
Hao Liang,Shanshan Sun,Yan Zhou,Yanhui Liu
In this study, a one-step and facile immobilization of enzymes by self-assembly of zinc ions and adenine in aqueous solution with mild conditions was reported. Enzymes, such as glucose oxidase (GOx) and horseradish peroxidase (HRP), could be efficiently encapsulated in Zn/adenine coordination polymers (CPs) with high loading capacity over 90%. When the enzyme was immobilized by CPs, it displayed high catalytic efficiency, high selectivity and enhanced stability due to the protecting effect of the rigid framework. As a result, the relative activity of Zn/adenine nano-CP-immobilized GOx increased by 1.5-fold at pH 3 and 4-fold at 70 to 90 °C, compared to free GOx. The immobilized GOx had excellent reusability (more than 90% relative activity after being reused eight times). Furthermore, the use of this system as a glucose biosensor was also demonstrated by co-immobilization of two enzymes, detecting glucose down to 1.84 µM with excellent selectivity. The above work indicated that in-situ self-assembly of Zn/adenine CPs could be a simple and efficient method for biocatalyst immobilization.Keywords:adenine;enzyme;self-assembly;coordination polymers;immobilization
研究领域:
生物化学
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纳米生物学
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酶工程
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DOI
高影响因子
Nanoscale
·
2021
·Vol. 13
·期 42
·17871-17880
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IF 7.79
Wenjing Qi,Huimin Yu
Surface immobilization of enzymes on magnetic-recoverable carriers is of great interest and importance for the biocatalysis of relatively large molecules. In this work, the nanosized amino-rich filamentous M13 virus, a versatile biological scaffold, was applied as the unique soft backbone for lipase immobilization. Based on the structure and capsid proteins of M13 phages, the magnetic-recoverable mimic-free-lipases (MFLs) composed of the M13 hydrogels and magnetic particles were developed in two designs. In the first design, nanosized wild M13 phages were crosslinked into a phage hydrogel through the N-terminals of pVIII peptides while NH2-Fe3O4 magnetic nanoparticles (MNPs) were attached to the M13 virus through glutaraldehyde, forming the M13-(NH2-Fe3O4) magnetic phage hydrogel. In the second design, special M13 with Fe3O4 affinity pIII-peptide (FAP-M13) was biopanned for strongly binding towards bare Fe3O4 with the “hook”-like pIII-peptide (N-LPLSTQH-C). TEM observation confirmed the direct grasp of FAP-M13 on bare Fe3O4, forming the magnetic (FAP-M13)-Fe3O4 virus hydrogel. Lipases were uniformly anchored on the phage surface of nanoscale by crosslinking with the N-terminals of pVIII peptides, and then lipase@M13-(NH2-Fe3O4) and lipase@(FAP-M13)-Fe3O4 MFLs were constructed. For both MFLs, high activity recovery yield (>95%) and efficient magnetic separation were characterized. Significantly reduced MNP-usage-amount and enhanced lipase-loading-amount both by about 40 folds were obtained, compared with the conventional NH2-Fe3O4 carriers. The quantified Km and Vmax/Km values were almost equal to those of the free lipases, verifying free-enzyme-mimicking features of the MFLs. High pH-tolerance, wide temperature adaptability, enhanced thermal stability and stable magnetic separation capability of both MFLs were also observed. In particular, the (FAP-M13)-Fe3O4 magnetic virus hydrogel simply using bare Fe3O4 MNPs would be more convenient and economical in the scaled-up biocatalysis.
研究领域:
生物技术
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材料科学
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生物化学
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化学工程
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DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2018
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IF 3.235
Xuejiao Liu,Wanwan Chen,Meiling Lian,Xu Chen,Yanluo Lu,Wensheng Yang
Metal-organic frameworks (MOFs) as a type of potential matrix for enzyme immobilization have attracted much interest. However, the sensitivity of most MOF-enzymatic electrochemical biosensors has not been satisfactory so far due to the poor conductivity and low apparent affinity of MOFs. In this work, new strategies have been presented: (i) using MOFs that themselves possess catalytic properties for substrates, so the synergistic catalysis by combining the MOFs and immobilized enzymes can be reinforced; (ii) introducing hydrophilic carbon nanomaterials and preparing MOF/carbon composites, not only the conductivity of the matrix but also the apparent affinity of immobilized enzyme for hydrophilic substrate can be remarkably enhanced. Horseradish peroxidase (HRP) and zeolite imidazolate framework-67(Co)/multi-walled carbon nanotube composite (ZIF-67(Co)/MWCNT) were adopted as the model enzyme and the immobilized matrix to illustrate and examine the proposed strategies. Compared with single component (ZIF-67(Co) or MWCNTs) based HRP electrochemical biosensors, the composite electrochemical biosensor displayed increased apparent substrate affinity (Michaelis constant K m reduced by ~75%) and 1.3-fold increased sensitivity in the detection of H 2 O 2 . The sensitivity (315 μA mM −1 cm −2 ) was also much larger than that of most previously reported MOF-HRP electrochemical biosensors. The proposed strategies are envisaged to be useful for other MOF-enzymes systems, which will be beneficial to the development of biocatalysis and biosensing.
研究领域:
生物化学
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材料科学
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分析化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
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IF 5.162
Sizhu Ren,Ziyuan Wang,Muhammad Bilal,Yuxiao Feng,Yunhong Jiang,Shiru Jia,Jiandong Cui
Multienzymatic conversion of carbon dioxide (CO 2 ) into chemicals has been extensively studied. However, regeneration and reuse of co-factor are still the main problems for the efficient conversion of CO 2 . In this study, a nanoscale multienzyme reactor was constructed by encapsulating simultaneously carbonic anhydrase (CA), formate dehydrogenase (FateDH), co-factor (NADH), and glutamate dehydrogenases (GDH) into ZIF-8. In the multienzyme reactors, cationic polyelectrolyte (polyethyleneimine, PEI) was doped in the ZIF-8 by dissolving it in the precursors of ZIF-8. Co-factor (NADH) was anchored in ZIF-8 by ion exchange between PEI (positive charge) and co-factor (negative charge), and regenerated through GDH embedded in the ZIF-8, thus keeping high activity of FateDH. Activity recovery of FateDH in the multienzyme reactors reached 50%. Furthermore, the dissolution of CO 2 in the reaction solution was increased significantly by the combination of CA and ZIF-8. As a result, the nanoscale multienzyme reactor exhibited superior capacity for conversion of CO 2 to formate. Compared with free multienzyme system, formate yield was increased 4.6-fold by using the nanoscale multienzyme reactor. Furthermore, the nanoscale multienzyme reactor still retained 50% of its original productivity after 8 cycles, indicating excellent reusability.
研究领域:
生物技术
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酶工程
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2021
·
IF 10.588
Yang Ruan,Huimin Zhang,Zijing Yu,Zenghui Diao,Gang Song,Minhua Su,Li’an Hou,Diyun Chen,Shuao Wang,Lingjun Kong
Uranium (U) immobilization from wastewater by zero valent iron (ZVI) was widely concerned through reduction and surface adsorption. Releasing of U due to re-oxidation of U(IV) into U(VI) limited the application of ZVI in U decontamination . In this work, a kind of biochar supported nano zero valent iron (Fe/BC(900)) was obtained by carbothermal reduction of starch mixed with ferric nitrate at 900 °C. U immobilization behavior by Fe/BC(900) in the presence of phosphate (P) was investigated. The U immobilization reaction was adjusted by controlling the sequence of U, Fe/BC(900) and P. U immobilization efficiency was enhanced to 99.9% in the presence of P. Reaction sequence of U, Fe/BC(900) and P influenced the U immobilization efficiency, which followed the order of (U-P)+Fe/BC(900)>(U- Fe/BC(900))+P>U+Fe/BC(900)>(P-Fe/BC(900))+U. P and nZVI both contributed to enhancing U immobilization through precipitation of uranyl-P and reductive co-precipitate (U(IV)) in a wide pH range. The released Fe ions could precipitate with uranyl and phosphate. Consumption of P and nZVI in the (P-Fe/BC(900))+U system limited U immobilization ability. The precipitate is highly dependent on U, P and Fe elements. U desorption in (U-P)+Fe/BC(900) system was not observed with stability.
研究领域:
环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Biointerphases
·
2023
·Vol. 18
·期 4
·
IF 2.1
Wang Qi,Fu Hao,Qi Xiaoyu,Zhang Lei,Ma Hongyan
Horseradish peroxidase (HRP) is a hemoglobin composed of a single peptide chain that catalyzes the oxidation of various substrates such as phenol and aniline in the presence of hydrogen peroxide via its iron-porphyrin catalytic center. This enzyme is widely used in industrial phenol removal, food additives, biomedicine, and clinical test reagents due to its rapid reaction rate and obvious reaction outcomes. However, the large-scale use of HRP in industrial applications still faces numerous challenges, including activity, stability, and sustainability. This study demonstrates that when peroxidase is immobilized in zwitterionic polymer hydrogels, polycarboxybetaine (PCB) and polysulfobetaine (PSB), the properties of the enzyme are improved. PCB and PSB-embedded HRP exhibit a 6.11 and 1.53 times increase in Kcat/Km value, respectively, compared to the free enzyme. The immobilized enzyme also experiences increased activity over a range of temperatures and better tolerance to extreme p H and organic solvents, including formaldehyde. In addition, immobilized HRP exhibits excellent performance in storage and reproducibility. Remarkably, PCB-HRP still retains 80% of the initial activity after a 6-week storage period and can still attain the initial catalytic level of the free enzyme after six repeated cycles. It also removes 90% of phenol within 12 min, surpassing the current pharmacy on the market. These experimental results indicated that we have successfully designed a set of stable and efficient support substrates for horseradish peroxidase, which enhances its suitability for deployment in industrial applications.
研究领域:
生物化学
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环境科学
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工业酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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