Scientific Reports
·
2025
·Vol. 15
·期 1
·1-16
·
IF 3.9
Chen Chunxia,Huang Huan,Zhang Haofeng,Huang Junli,Zhou Xing,Xie Xixiang,Liao Xiaoling,Zhou Yao,Cai Fangqi,Chen Guirong,Xie Yuping,Huang Luying
The TLR4/NLRP3 inflammasome pathway drives lung cancer cell proliferation, migration, and invasiveness. However, no in vivo treatments targeting this pathway in lung cancer exist. Resveratrol, an anti-inflammatory compound, inhibits the NLRP3 inflammasome but requires high doses due to its hydrophobicity and instability. This study aimed to enhance the therapeutic efficacy of resveratrol by encapsulating it in liposomes to effectively target the TLR4/NLRP3 inflammasome pathway in lung cancer. In this study, the efficacy of resveratrol liposome was tested in vitro through cell proliferation and migration assays and gene expression analysis. In vivo effects were evaluated in a lung cancer mouse model using histological and molecular techniques. Resveratrol liposome significantly inhibited lung cancer cell proliferation and migration, and induced apoptosis in orthotopic lung cancer models. Additionally, by suppressing TLR4/NLRP3 inflammasome-related genes, resveratrol liposomes diminished levels of IL-1β and IL-18 both locally at tumor sites and systemically, thus modulating the tumor immune microenvironment by decreasing MDSCs and increasing CD4 + and CD8 + T cells. In conclusion, resveratrol liposome alleviates lung cancer progression by targeting the TLR4/NLRP3 inflammasome pathway and enhancing anti-tumor immune responses, highlighting its potential as a promising therapeutic strategy for lung cancer treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CELL BIOCHEMISTRY AND BIOPHYSICS
·
2025
·
IF 2.5
Wang Hong,Li Feilong,Mei Yongliang,Wu Taotao,Wang Liquan,Hao Pandeng,Ci Shenjing,Liu Zongchao
Intervertebral disc degeneration (IVDD), characterized by lower back pain, is a prevalent chronic orthopedic disease imposing significant physical and economic burdens. However, the molecular mechanisms of IVDD remain insufficiently explored, hindering clinical guidance. This study aimed to identify key targets of IVDD via bioinformatics, providing new therapeutic directions. Gene expression profiles GSE34095 and GSE150408 were retrieved from public databases, and IVDD-related gene datasets were obtained from GeneCards and CTD. Protein-protein interaction (PPI) networks were constructed, followed by functional enrichment analyses using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome Pathway Knowledgebase (Reactome), and WikiPathways. Key genes associated with the ferroptosis pathway were screened, and the effect of resveratrol on IVDD was validated via in vitro and in vivo experiments. Enrichment analyses identified ferroptosis as the major signaling pathway in IVDD. Six key genes (Glutathione Peroxidase 4 (GPX4), Transferrin receptor (TFRC/TFR1), Solute Carrier Family 7 Member 11 (SLC7A11/xCT), Hypoxia Inducible Factor 1 Subunit Alpha Gene (HIF1A), Interleukin-6 (IL6), and Interleukin-1 Beta (IL1B)) were selected. In vitro studies showed that resveratrol inhibited ferroptosis in nucleus pulposus cells (NPCs), while in vivo experiments in rats confirmed that resveratrol alleviated IVDD by regulating ferroptosis-related genes. Molecular docking validated the binding of resveratrol to these key targets. This study demonstrates that resveratrol delays IVDD progression by inhibiting ferroptosis in NPCs, providing a novel therapeutic strategy for clinical IVDD prevention and treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
·
2024
·
IF 1.9
Liu Lilong,Wang Yexin,Dong Yanyan,Lin Shan,Guan Wenhui,Song Jia
The clinical application of 5-fluorouracil (5-Fu), a potent chemotherapeutic agent, is often hindered by its well-documented cardiotoxic effects. Nevertheless, natural polyphenolic compounds like resveratrol (RES), known for their dual anti-tumor and cardioprotective properties, are potential adjunct therapeutic agents. In this investigation, we examined the combined utilization of RES and 5-Fu for the inhibition of gastric cancer using both in vitro and in vivo models, as well as their combined impact on cardiac cytotoxicity. Our study revealed that the co-administration of RES and 5-Fu effectively suppressed MFC cell viability, migration, and invasion, while also reducing tumor weight and volume. Mechanistically, the combined treatment prompted p53-mediated apoptosis and autophagy, leading to a considerable anti-tumor effect. Notably, RES mitigated the heightened oxidative stress induced by 5-Fu in cardiomyocytes, suppressed p53 and Bax expression, and elevated Bcl-2 levels. This favorable influence enhanced primary cardiomyocyte viability, decreased apoptosis and autophagy, and mitigated 5-Fu-induced cardiotoxicity. In summary, our findings suggested that RES holds promise as an adjunct therapy to enhance the efficacy of gastric cancer treatment in combination with 5-Fu, while simultaneously mitigating cardiotoxicity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Jun Yang,Jieqiong Lin,Weidong Zhang,Mingyue Shen,Yuanxing Wang,Jianhua Xie
BACKGROUND Resveratrol (Res) is promising food functional factor with favorable antioxidant and anti-inflammatory properties, although its poor water solubility and low bioavailability limit extensive application. Therefore, in combination with another promising polysaccharide ( Mesona chinensis polysaccharides, MCP), Res-loaded food nanocarriers (ResNPs) were developed to increase its water solubility, bioactivity and targeting properties. ResNPs were then applied to alleviate dextran sulfate sodium (DSS)-induced ulcerative colitis. RESULTS Resveratrol can be well encapsulated in MCP-based nanoparticles in an amorphous state, improving its water solubility. ResNPs showed pH-response controlled release behavior in the gastrointestinal tract and increased the enrichment of Res in the colon. In vivo experiments of ResNPs against DSS-induced ulcerative colitis (UC) revealed that ResNPs significantly improved UC symptoms, modulated intestinal inflammation and down-regulated oxidative stress levels compared to free Res. ResNPs also play an positive role with respect to inhibiting the mitogen-activated protein kinase pathway and promoting the expression of tight junction proteins. In addition, ResNPs improved the species composition and relative abundance of intestinal flora in UC mice, which effectively regulated the balance of intestinal flora and promoted the production of short-chain fatty acids. CONCLUSION These results suggest that MCP-based nanoparticles can effectively improve the solubility of resveratrol and enhance its in vivo bioactivity. Moreover, the present study also provides a new strategy for the prevention and treatment of UC with food polyphenol. © 2024 Society of Chemical Industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Mofei Han,Kaiqi Tang,Zhenhua Chen
Periodontitis, as a chronic inflammatory oral disease, is the primary cause of tooth loss. The oxidative stress microenvironment of periodontitis causes damage of both osteoblasts’ mitochondrion and periodontal tissue. Natural extracts with antioxidant properties, including resveratrol (RSV), have been reported in regulating oxidative stress microenvironment, however, the controlled release of them in periodontal tissue is still a challenge. Herein, a smart photothermal-responsive gold nanocages hydrogel (RSV-Au@H) was constructed for the treatment of periodontitis by controlling the release of resveratrol. Under the near-infrared (NIR) light irradiation for 5 min, RSV-Au@H could be heated to 45.8 ℃ due to the good photothermal effect of Au nanocages encapsulated in the hydrogel. This NIR stimulated photothermal effect of RSV-Au@H could control the release of RSV. Molecular biology indicated that RSV-Au@H could scavenge ROS with 42 % efficiency. Transmission electron microscopy (TEM) showed a significant improvement in osteoblasts mitochondrial morphology. Micro-CT scanning revealed that RSV-Au@H reduced the distance between cemento-enamel junction and alveolar bone crest (CEJ-ABC) by up to 55 % and increased bone volume fraction by about 48 %. These results suggested that this multifunctional nanoplatform with NIR controlled release of RSV might be a new therapeutic strategy for periodontitis.
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2022
·
IF 5.112
Lu Zhang,Zhaomin Lin,Yuan Chen,Dongfang Gao,Peng Wang,Yuxing Lin,Yongmei Wang,Fang Wang,Ying Han,Huiqing Yuan
Combination therapy is frequently used in cancer treatments. Delivery of combined anticancer agents loaded in a nanocarrier would be a promising option for combination therapy. Here, we designed PEGylated nano-liposomes for co-delivery Docetaxel (Doc) and Resveratrol (Res) to evaluate antitumor efficiency of the combined drugs in prostate cancer. The average diameter of the liposomes was 99.67 nm with a spheral-like shape. Drug release studies showed that both drugs could synchronously leak from the liposomes in a sustained release behavior. Cellular uptake results demonstrated that liposomes could effectively deliver more cargos into cells than other formulations. Moreover, co-loaded liposomes with Doc/Res in a molar ratio of 1:2 exhibited significantly higher cytotoxicity than a mixed solution containing both drugs on cancer cells. In the study of caspase 3, we found that the combination of Doc and Res could significantly increase the activity of caspase 3 enzyme compared with Doc alone. Animal studies revealed that co-encapsulated Doc/Res in liposomes predominantly inhibited tumor growth in PC3 bearing Balb/c nude mice, as evidenced by a change in cell proliferation and apoptosis parameters. Importantly, little toxicities and prolonged survival time were observed in mice treated with liposome-loaded Doc/Res than control group exposed to liposome-free Doc/Res. These results provided evidence that loading of Doc/Res in a nano-liposome is an efficient delivery formulation for synergistic treating prostate cancer.
✓ 已验证
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2019
·
IF 5.839
Linlin Li,Hualin Wang,Minmin Chen,Suwei Jiang,Junfeng Cheng,Xingjiang Li,Mingzhu Zhang,Shaotong Jiang
Resveratrol (R) has received growing attentions in therapeutic potential, however less focus is paid to its antibacterial or antioxidant nature for food preservation. In this work, R was efficiently encapsulated in electrospun gelatin/zein (GA/ZN) fibers for developing antibacterial mats to extend pork shelf life. The fiber morphology, thermal stability and hydrophobicity of the mats were associated with the ratios of GA/ZN. The secondary structure changes of protein were dependent on the intermolecular hydrogen bonds formed in the fiber matrix. The presence of R endowed the mats with antibacterial and antioxidant activity and the release of R followed Fickian diffusion mechanism in 10% ethanol and the coexistence of diffusion and erosion mechanisms in 3% acetic acid, respectively. The target fiber mats could extend the pork shelf life by 3 d at 4 °C, indicating the good application prospect in food preservation.
✓ 已验证
DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2013
·Vol. 97
·期 23
·9941-9954
·
IF 3.689
Zhang Jinhua,Shi Junling,Liu Yanlin
To identify the substrates and enzymes related to resveratrol biosynthesis in Alternaria sp. MG1, different substrates were used to produce resveratrol, and their influence on resveratrol production was analyzed using high performance liquid chromatography (HPLC). Formation of resveratrol and related intermediates was identified using mass spectrum. During the biotransformation, activities of related enzymes, including phenylalanine ammonia-lyase (PAL), trans -cinnamate 4-hydroxylase (C4H), and 4-coumarate-CoA ligase (4CL), were analyzed and tracked. The reaction system contained 100 mL 0.2 mol/L phosphate buffer (pH 6.5), 120 g/L Alternaria sp. MG1 cells, 0.1 g/L MgSO 4 , and 0.2 g/L CaSO 4 and different substrates according to the experimental design. The biotransformation was carried out for 21 h at 28 °C and 120 rpm. Resveratrol formation was identified when phenylalanine, tyrosine, cinnamic acid, and p -coumaric acid were separately used as the only substrate. Accumulation of cinnamic acid, p -coumaric acid, and resveratrol and the activities of PAL, C4H, and 4CL were identified and changed in different trends during transformation with phenylalanine as the only substrate. The addition of carbohydrates and the increase of phenylalanine concentration promoted resveratrol production and yielded the highest value (4.57 μg/L) when 2 g/L glucose, 1 g/L cyclodextrin, and phenylalanine (4.7 mmol/L) were used simultaneously.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2015
·
IF 2.912
Qian Lu,Qin Zhao,Qiong-Wei Yu,Yu-Qi Feng
In this study, a simple and convenient method for the determination of trans-resveratrol (TRA) in peanut oils based on pollen grain solid-phase extraction (SPE) was developed. Pollen grains were used as normal-phase SPE sorbent to separate TRA from peanut oils for the first time. As a naturally occurring material, pollen grains exhibited an excellent adsorption capacity for polyphenolic compounds due to their particular functional structures such as hydroxyl groups, saturated and unsaturated aliphatic chains with aromatics. Their stable compositions as well as adequate particle size (30–40 μm) also make them suitable for SPE. Several parameters influencing extraction performance were investigated. Coupled with high-performance liquid chromatography-ultraviolet detection (HPLC-UV), a green purification method for fast determination of TRA in peanut oils using pollen grain cartridges as sorbents was established. The linearity range of the proposed method was 10–2500 ng·g–1 with a satisfactory correlation coefficient (r2) of 0.9999. The limit of detection (LOD) for TRA in peanut oils was 2.7 ng·g–1, and the recoveries in spiked oil samples were from 70.2% to 98.4% with the relative standard deviations (RSDs) less than 4.9% (intraday) and 5.2% (interday). This method was successfully applied to the analysis of TRA in several peanut oils with different brands from local market as well as other kinds of vegetable oils.
✓ 已验证
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Yuxuan Liu,Qiufang Liang,Xueqiong Liu,Husnain Raza,Haile Ma,Xiaofeng Ren
The study was conducted to understand the effect of zein-gum Arabic (GA) complex coacervates prepared by ultrasound treatment on the encapsulation of resveratrol. Compared with zein, zein-GA complex coacervation largely increased the encapsulation efficiency from 26.19% to 55.85% and it was further increased to 74.2% after sonication. Also, prominent rise in the related loading capacities from 2.79% to 6.01% was noted. The increased particle size by the addition of GA and polydispersity index of the ternary complex was dramatically reduced after ultrasound treatment; the absolute values of zeta potential were increased. The hydrogen bonds, electrostatic interactions and hydrophobic effects were the main driving forces for the formation of the ternary complex particles. Ultrasound treatment strengthened the hydrogen bonding and electrostatic interactions, resulting in the formation of compact and homogeneous composite nanoparticles with smaller particle size, which was further confirmed by scanning electron microscope and atomic force microscope. These findings reveal the mechanism of ultrasound enhancing the zein-GA complex coacervation to load resveratrol, and demonstrate its promising effect in modifying protein-polysaccharide complex coacervation as a delivery carrier in the functional food and beverage industry.
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Jian-Dong Wang,Li-Na Fu,Li-Tao Wang,Zi-Hui Cai,Yan-Qiu Wang,Qing Yang,Yu-Jie Fu
Resveratrol, as a natural antioxidant, has been studied widely for its antioxidant, anti-inflammatory, anti-apoptotic, and anticancer properties. In this study, a strategy was developed to transform and extract resveratrol from Polygonum cuspidatum on the basis of NADES (natural deep eutectic solvent). In the series of adjustments and optimizations by single-factor experimental, the optimum NADES system (choline chloride to oxalic acid was 1:1) and other experimental conditions were determined, including water content of NADES, solid-liquid ratio, extraction time, extraction temperature and ultrasonic power. At the same time, RSM combined with BBD further optimized the experimental process, the results obtained by RSM optimization showed that the resveratrol yield of 12.31 mg/g was achieved when solid-liquid ratio was 1:50, temperature was 75℃ and time was 80 min. Meantime, the conversion efficiency of polydatin was 96.11 % and the content of resveratrol increased 6-fold higher than that of untreated sample. The kinetic of extraction and thermodynamic analysis were performed at different extraction temperatures to examine the temperature on the impact of the resveratrol extraction from P. cuspidatum . Ethyl acetate was used for back extraction in order to achieve the recycling of solvent and the recovery of resveratrol from NADES. The results showed that ethyl acetate had a good extraction effect and NADES still had a high capacity to convert and extract resveratrol after three cycles. In addition, the extracts exhibited superior antioxidant activity with higher content of recovered resveratrol. This established process can therefore be used to extract and recover resveratrol from plants as an environmental, pollution-free alternative approach.
✓ 已验证
DOI
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2024
·Vol. 28
·期 8
·2669-2677
·
IF 2.6
Xia Xiaohong,Duolihong Bawurejiang,Ma Xiangdong,Yue Shan,Liu Rui
Electrochemical biosensor based on horseradish peroxidase (HRP) modified gold nanoparticle/sulfur-doped graphene (AuNP/SG) has been developed for the quantification of trans -resveratrol (Res). Gold nanoparticle (AuNP) was selected as the carrier for HRP due to its excellent electrochemical catalytic activity, biocompatibility, and conductivity, while sulfur-doped graphene (SG) was selected as the support for AuNP due to the defects introduced in the carbon network by sulfur doping can facilitate the high dispersion state of AuNP and HRP. The results show that the excellent sensing performance of the HRP/AuNP/SG is attributed to the high charge polarization of material after sulfur dropping and the co-catalysis of HRP and AuNP. For the detection of Res, linear response can be observed with high sensitivity and low detection limit (4 × 10 −8 M). Further, this electrochemical biosensor presents strong reproducibility, stability, and anti-interference capability in detecting Res. These unique properties make it an excellent choice for Res reliable detection.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）