INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 16
·7702
·
IF 4.9
Xinyue Jiang,Liang Fei,Yayun Yang,Jiao Han,Zhaoxin Tang,Jianzhao Liao,Lianmei Hu,Ying Li,Jiaqiang Pan
Pulmonary hypertension syndrome (PHS), a metabolic disorder causing economic losses in broilers, arises from hypoxia-induced portal hypertension and liver cirrhosis, triggering mitochondrial oxidative damage, excessive ROS production, and altered mitochondrial biogenesis. This study explored terpinen-4-ol (T4O), known for antimicrobial and anti-inflammatory properties, in mitigating PHS. Broilers were divided into four groups, including PHS-affected birds with/without T4O supplementation. Analyses revealed that PHS birds exhibited reduced antioxidant capacity, elevated MDA and ROS levels, increased mitochondrial numbers, and upregulated expression of oxidative stress markers (Keap1, HO-1, Nrf-2) and mitochondrial biogenesis regulators (PGC-1α, Nrf-1, Tfam). T4O administration enhanced antioxidant activity, reduced ROS and MDA, suppressed compensatory mitochondrial proliferation, and downregulated Keap1/Nrf-2 and mitochondrial biogenesis pathways. These effects suggest that T4O alleviates hypoxia-driven oxidative stress and mitochondrial dysfunction in broilers. Findings highlight T4O’s potential as a therapeutic agent to mitigate PHS-related losses in poultry production.
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高影响因子
DESALINATION
·
2025
·
IF 9.8
Xifan Li,Guijing Chen,Xin Li,Zhaoyang Song,Wen Liu,Baicang Liu
The flourishing growth of electric vehicle market has led to pile of spent lithium-ion batteries (LIB), efficient treatment and recovery of retired LIBs is crucial for ecological environment and human health, especially the terminal treatment of spent lithium-ion battery raffinate (SLR). For selectively recycle Li + from SLR (the lithium and sodium concentration reaches up to 1000 mg/L and 31,200 mg/L, respectively), dibenzo 14-crown-4 ether (DB14C4) is synthesized as extractant for lithium due to the excellent size-match effect. The extraction performance of DB14C4 is evaluated from aspects of dosage, temperature, organic/aqueous phase (O/A) ratio, adsorption kinetics, adsorption selectivity and reusability through liquid-liquid extraction. The results manifested that DB14C4 exhibited fast adsorption kinetics and reached saturation in 1 h, and reached the highest adsorption capacity of 22.1 mg/g with concentration of 0.03 mol/L, O/A ratio of 1:4 and temperature of 25 °C. Besides, DB14C4 has excellent selective adsorption for Li + with lithium partition coefficient of 10.2 mL/g and Li + /Na + separation factor of 13.2. Furthermore, the selectivity of DB14C4 to Li + is studied by density functional theory calculation, including structure optimization, molecular orbital analysis, charger transfer and natural bond orbital (NBO) analysis. The results show that DB14C4 preferentially complex with Li + with lithium ion lies in the center plane of crown ether ring, and the DB14C4-Li + complex has higher charge transfer and bigger stabilization energy than DB14C4-Na + . This paper provides a feasible method for lithium recovery from SLR to guarantee sustainable lithium supply for electric vehicles, and offers brand-new perspectives to evaluate the full life cycle of LIBs.
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产品关联
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CHEMOSPHERE
·
2022
·
IF 8.943
Man Qu,He Chen,Hanpeng Lai,Xing Liu,Dayong Wang,Xing Zhang
Nanoplastics represented by nanopolystyrene (NPS) and its chemically modified derivatives are environmentally ecotoxicological hotpots in recent years, but their toxicity and underlying mechanisms have not been fully identified. Here we employed Caenorhabditis elegans as an animal model to systematically compare the toxicity between nanopolystyrene and its 4 chemically modified derivatives (PS-PEG, PS-COOH, PS-SOOOH and PS-NH 2 ) at predicted environmental concentrations. Our study demonstrated that compared with PS exposed group, PS-NH 2 exposure (15 μg/L) caused a significant decline in lifespan by suppressed DAF-16/insulin signaling and shortened body length by inhibiting DBL-1/TGF β signaling. Different from PS-NH 2 exposed group, PS-SOOOH exposure (15 μg/L) could not cause changes in lifespan, but shortened body length by inhibiting DBL-1/TGF β signaling. In addition, PS-COOH, PS-SOOOH or PS-NH 2 exposure (1 μg/L or 15 μg/L) caused more serious toxicity in reducing locomotion behavior and causing gut barrier deficit. Hence the rank order in toxicity of PS-NH 2 ＞PS-SOOOH＞PS-COOH＞PS＞PS-PEG was identified. Furthermore, we also presented evidence to support the contention that the observed toxic effects on nematodes were linked to oxide stress and activation of anti-oxidative molecules for reversing the adverse effects induced by nanopolystyrene and its 4 chemically modified derivatives. Our data highlighted nanoplastics may be charge-dependently toxic to environmental organisms, and the screened low toxic modification may support polystyrene nanoparticles continued application for daily consumer goods and biomedicine.
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PROTEIN EXPRESSION AND PURIFICATION
·
2018
·
IF 1.338
Hualong Xi,Jinfei Yu,Qing Sun,Jingcai Lu,Tiejun Gu,Xiaonan Guo,Bo Li,Xiaorui Chen,Kaixin Zhang,Wei Kong,Yongge Wu
Streptococcus pneumoniae is a major pathogen that causes life-threatening diseases, such as pneumonia, otitis media , bacteremia , and meningitis, worldwide and especially in young children and the elderly. Pneumococcal surface protein A (PspA) is a widely studied candidate protein vaccine that represents a promising replacement for current polysaccharide and polysaccharide-conjugate vaccines. In this study, we describe a simple method to produce PspA of clade 4 from an Escherichia coli expression system using hydroxylapatite and ion-exchange chromatography. Using this method, we successfully expressed soluble PspA4 in 10 L of autoinducing culture medium, with a wet-cell yield of 19 g/L and a final PspA4 concentration of 22.8 mg/L. Additionally, we improved PspA4 purity from 17% to 70% in a single step through the use of hydroxylapatite, resulting in acquisition of recombinant PspA4 (>95% purity) at a final yield of 43% from the starting cell-lysis solution. We subsequently verified the secondary structure molecular weight of recombinant PspA4 by circular dichroism and mass spectrometry , respectively. These results demonstrated a highly efficient method for mass producing PspA4 protein and that can also be applied for purification of PspA proteins from other clades.
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高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
·
2020
·
IF 3.263
Shumin Jiang,Xueli Wu,Yi Wang,Jingtao Zou,Xiaoping Zhao
Dipeptidyl Peptidase-4 (DPP-4) is a specific enzyme hydrolyzing the incretin hormone glucagon like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) to reduce insulin secretion, meanwhile DPP-4 inhibitors play an important role in diabetic therapy. In present study, 14 potential inhibitors were screened with an inhibition over 50% on DPP-4 activity from Xiao-Ke-An formula (XKA) and 12 of them exhibited a dose-dependently inhibitory effect at concentrations of 5–50 μmol/l. We found 10 DPP-4 inhibitors restrained differentiation of 3T3-L1 pre-adipocytes as well as reducing the triglycerides and total cholesterol content in 3T3-L1 adipocytes. Furthermore, 7 DPP-4 inhibitors promoted the glucose consumption in insulin-resistance BNL CL.2 cells. Thereinto, ginsenoside Rk 1 up-regulated the protein kinase B (AKT) and glycogen synthase kinase-3 (GSK-3β) phosphorylation expression, while kukoamine B and coptisine hydrochloride obviously increased the phosphorylation of AKT protein and columbamine , panaxadiol , ginsenoside Ro , timosaponin AI significantly promoted the phosphorylation of GSK-3β protein. It's our first effort to confirm those seven compounds could serve as DPP-4 inhibitors to attenuate DPP-4 activities, accompanied with the ability to adjust glucolipid metabolism. Moreover, activating the AKT/GSK-3β signaling pathway to ameliorate insulin resistant may be the anti-diabetic mechanism of XKA.
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REACTIVE & FUNCTIONAL POLYMERS
·
2017
·
IF 3.151
Zhenfeng Huang,Runqi Liuyang,Chengyun Dong,Yufeng Lei,Anqiang Zhang,Yaling Lin
Polymeric quaternary ammonium salts (PQAS) have been widely used to prevent microbial contamination, but little is known about their activity against phytopathogenic fungi. Our previous report described the synthesis of two novel PQAS, namely a homopolymer of (2-methacrylamido) propyltetrabenzyldimethylammonium chloride (PQD-BC) and dimethylaminopropyl benzyl chloride-grafted polysiloxanes (PDMS-g-BC); we demonstrated their structure-activity relationship against phytopathogenic fungi such as R . solani and Fusarium oxysporum f. sp. cubense tropical race 4 (Foc4). Here, we analyzed the structure-activity relationship and toxicity mechanism of these compounds at the molecular level against Foc4 compared with the low molecular-weight quaternary ammonium salt benzalkonium chloride (BC). The results revealed that PQD-BC and PDMS-g-BC application inhibited the growth of Foc4 in a concentration-dependent manner and that PDMS-g-BC exhibited higher activity than PQD-BC. In addition, these polymers were found to induce cell death in Foc4 by disrupting the cellular structure integrity, such as the loss of the cell wall and plasma membrane integrity and oxidative stress (lipid peroxidation), leading to the release of intracellular contents and inducing mitochondrial dysfunction and interference with genomic DNA . The newly elucidated mechanism provides possible applications in which PQAS can be used against phytopathogenic fungi.
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CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Lujia Ding,Pengcheng Wang,Qiuhan Lin,Dongxue Li,Yuangang Xu,Ming Lu
Fused cyclic energetic compounds are a unique class of large conjugated structures containing two or more rings, and have been identified as promising competitors for traditional energetic materials. In the present work, we investigated 5-amino-6-oxo-4,6-dihydro-[1,2,5]oxadiazolo[3′,4′:5,6]pyrido[4,3- d ][1,2,3]triazine 8-oxide ( 3 ) in detail as a fused amphoteric energetic molecule that can be protonated and deprotonated by acids and bases to obtain its cationic ( 11 – 13 ) and anionic ( 4 – 10 ) salts. All the new compounds were thoroughly characterized by infrared and multinuclear NMR ( 1 H and 13 C) spectroscopy, and elemental analysis. The structural features of 3 – 6 , 9 , 10 , 12 , and 13 were investigated by single-crystal X-ray diffractions and different theoretical techniques (Hirshfeld surfaces analyses, and noncovalent interactions). The aromaticity of compound 3 was studied via localized orbital locator- π and iso -chemical shielding surfaces (ICSS). Their thermal decomposition temperatures were investigated by differential scanning calorimetry and thermogravimetry to be 310–344 °C (onset). In addition, impact and friction tests revealed that these compounds were insensitive. Densities, heats of formation and detonation properties were measured by a gas pycnometer, calculated using the Born-Haber energy cycle and EXPLO5 v6.05.04 program, respectively. The neutral compound 3 and all its non-metallic salts have better detonation properties than HNS and PYX and lower sensitivities. In particular, salt 11 (N + O: 61.74 %, T d : 330 °C, Δ H f : 1.68 kJ g −1 , D : 8.795 km s −1 , P : 36.0 GPa, IS: > 40 J, FS: > 360 N) is a potential candidate for heat-resistant explosives.
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JOURNAL OF CHEMICAL RESEARCH
·
2018
·
IF 0.668
Song Bai,Shan Liu,Yunying Zhu,Jiali Lu,Lina Ai,Jing Xue,Qin Wu
An efficient asymmetric synthesis of chiral β-amino acid ester derivatives containing a 4-(pyridin-3-yl)pyrimidin-2-yl amine moiety was developed. This catalytic asymmetric Mannich reaction gave target products in high yields (95%) and excellent enantioselectivities (>99% ee) using a cinchona-based squaramide catalyst under solvent-free, one-pot conditions. Antiviral bioassays indicated that some of the chiral products exhibited higher antiviral activities against tobacco mosaic virus than the commercial compound ribavirin.
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高影响因子
MOLECULES
·
2017
·Vol. 22
·期 5
·783
·
IF 2.861
Khalid Khan,Syed Lal Badshah,Nasir Ahmad,Haroon Ur Rashid,Yahia Mabkhot
The inclusion complexes of a new family of nonionic amphiphilic calix[4]arenes with the anti-inflammatory hydrophobic drugs naproxen (NAP) and ibuprofen (IBP) were investigated. The effects of the alkyl chain’s length and the inner core of calix[4]arenes on the interaction of the two drugs with the calix[4]arenes were explored. The inclusion complexes of Amphiphiles1a–cwith NAP and IBP increased the solubility of these drugs in aqueous media. The interaction of1a–cwith the drugs in aqueous media was investigated through fluorescence, molecular modeling, and1H-NMR analysis. TEM studies further supported the formation of inclusion complexes. The length of lipophilic alkyl chains and the intrinsic cyclic nature of cailx[4]arene derivatives1a–cwere found to have a significant impact on the solubility of NAP and IBP in pure water.Keywords:amphiphilic calix[4]arene;naproxen;ibuprofen;solubility;inclusion complexes
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高影响因子
Food & Function
·
2019
·Vol. 10
·期 11
·7052-7062
·
IF 3.241
Lukai Ma,Guoqin Liu,Weiwei Cheng,Xinqi Liu,Huifan Liu,Qin Wang
In this study, the formation of two toxic reactive aldehydes, 4-hydroxy-2-hexenal (HHE) and 4-hydroxy-2-nonenal (HNE), was investigated during frying of two different foodstuffs at 180 °C for 7 h in three different vegetable oils. The results showed that HHE and HNE content in the oil after frying was lower than that in the oil fried without foods. It was mainly because of the incorporation of HHE/HNE into the fried foods. In French Fries (FF), the HNE content was higher, whereas it was lower in the fried chicken breast meat (FCBM). The bidirectional model systems consisting of the model oil frying system and the model food frying system were used. The result of the model oil system showed that the content of HNE was higher in FF for the higher hydrophobic property than that in HHE, which would be preferably bounded into the hydrophobic helical structures, whereas the lower content of HNE was observed in FCBM due to its higher reactivity towards the nucleophilic group, namely, the protein in FCBM. Furthermore, the model food frying system including starch and protein extracted from the corresponding foodstuffs verified the results in the model oil system. Finally, the probable migration mechanism of HHE and HNE in different food matrices was proposed for the first time.
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JOURNAL OF STRUCTURAL CHEMISTRY
·
2022
·Vol. 63
·期 10
·1610-1615
·
IF 1.004
Wang G. -F.,Sun S. -W.
Abstract Two coordination polymers, {[Co 2 (L 1 ) 2 (adc) 2 ]·5H 2 O} n ( 1 ) and {[Ni(L 1 ) 2 (Hadc) 2 ]} n ( 2 ) (L 1 = bis(4-(1 H -imidazol-1-yl)phenyl)methanone, H 2 adc = adamantane-1,3-dicarboxylic acid), have been synthesized and structurally characterized. In 1 , Co1 is five-coordinated by three oxygen atoms of two adc 2– and two nitrogen atoms from two L 1 ligands. The neighboring [Co(adc)] 2 dimers are linked through four L 1 ligands to form a 1D ladder structure. In 2 , Ni1 is six-coordinated by two oxygen atoms from two Hadc – ligands, four nitrogen atoms from four distinct L 1 ligands to give a slight distorted octahedral geometry. The [Ni(Hadc) 2 ] units are bridged by L 1 ligands to form 1D looped chains.
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ZEITSCHRIFT FUR KRISTALLOGRAPHIE-NEW CRYSTAL STRUCTURES
·
2023
·Vol. 238
·期 6
·1069-1070
·
IF 0.3
Yan-Qing Wang,Wen-Qing Yan,Li-Xue Nie,Ping-Ping Luan,Chao-Qun Li
C 20 H 22 Cu 3 N 8 O 10 , monoclinic, C 2/ c (no. 15), a = 12.0766(11) Å, b = 16.2778(14) Å, c = 14.7605(13) Å, β = 97.8580(10) ∘ , V = 2874.4(4) Å 3 , Z = 4, R
gt ( F ) = 0.0420, wR
ref ( F
2 ) = 0.0988, T = 298.15 K.
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