JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2015
·
IF 3.368
Jing Zhao,Lei Yang,Ping Huang,ZhiYong Wang,Yan Tan,Hong Liu,JiaJia Pan,Cheng-Yi He,Zhi-Ying Chen
Gene therapy has held great promise for treating specific acquired and inherited diseases. However, the lack of safe and efficient gene delivery systems remains as the major challenge. Poly(β-amino ester)s (PBAEs) have attracted much attention due to their outstanding properties in biosafety, DNA delivery efficiency and convenience in synthesis. In this paper, we reported the further enhancement of the PBAE functions by increasing its positive charge through conjugating with low molecular weight polyethylenimine (LPEI). The resulted LPEI–PBAE polymer was able to condense minicircle DNA (mcDNA) forming nanoparticles with a diameter of 50–200 nm. Furthermore, as compared to parental PBAE and a commercial transfection reagent very common in laboratory application, the LPEI–PBAE demonstrated significantly higher transfection efficiency with little cytotoxicity. These results suggested LPEI–PBAEs are worthy of further optimization for gene therapy applications.
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RSC Advances
·
2015
·Vol. 5
·期 57
·46301-46306
·
IF 3.84
Jing Fang Guo,Rong Mei Fang,Cheng Zhi Huang,Yuan Fang Li
In order to eliminate the scattered light induced by the signal amplifier in fluorescence anisotropy (FA) assays, a nanosized metal–organic framework MIL-101, ranging from 80–500 nm, has been synthesized through a hydrothermal method with the addition of glycerol. We chose the 100 nm MIL-101 to enhance FA for label-free detection of the respiratory syncytial virus (RSV) gene sequence and the DNA-intercalating dye SYBR Green I (SGI) as the fluorophore, based on the different affinities of MIL-101 toward ssDNA and dsDNA. The nanosized MIL-101 has a negligible scattering effect owing to its smaller particle size, so all of the experimental data of FA values were smaller than the maximum initial anisotropy of 0.4. As a specific advantage, a dual amplification result of not only an increase in the FA value of SGI/ssDNA (r1) but also a decrease in the FA value of SGI/dsDNA (r2) was presented at the same time. Consequently, a larger FA value change Δr (Δr, Δr = r1 − r2) was obtained and contributed to improve the sensitivity. In addition, the quantitative detection of the target DNA (T) was achieved according to the relationship between Δr and the concentration of T. In the presence of MIL-101, the Δr is 7-fold higher than that without MIL-101 and achieved the sensitive and selective detection of RSV DNA.
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BIOSENSORS & BIOELECTRONICS
·
2013
·
IF 5.437
Sai Bi,Yangyang Cui,Ying Dong,Ningbo Zhang
A biosensing system is established for the multi-amplified detection of DNA or specific substrates of aptamers under isothermal conditions , which combines nicked rolling circle amplification (N-RCA) and beacon assisted amplification (BAA) with sensitive colorimetric technique by using DNAzymes as reporter units. According to the configuration, the analysis of DNA is accomplished by recognizing the target to capture nucleic acid-functionalized magnetic particles, followed by the self-assembly of the other two nucleic acids into multicomponent DNA supramolecular structure on magnetic particles. After magnetic separation, the circularization with ligase and the fragmentation with polymerase activate N-RCA and BAA in the presence of polymerase, dNTPs , and the nicking endonuclease , successively producing horseradish peroxidase (HRP)-mimicking DNAzymes that act as colorimetric reporter to catalyze the oxidation of ABTS 2− by H 2 O 2 in the presence of hemin . Under the optimized conditions, we obtain a wide dynamic range for DNA analysis over 6 orders of magnitude from 1.0×10 −14 to 1.0×10 −9 M with a low limit of detection of 6.8×10 −15 M. In the absence of a target, neither self-assembly of nucleic acids nor amplification process can be initiated, indicating an excellent selectivity of the proposed strategy. Similarly, an analogous system is activated by cancer cells or lysozyme through cooperative self-assembly of nucleic acids on magnetic particles in the presence of respective substrates of aptamers to synthesize HRP-mimicking DNAzymes that give the readout signal for the recognition events, achieving LODs of 81 Ramos cells and 7.2×10 −15 M lysozyme, respectively.
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ELECTROCHIMICA ACTA
·
2013
·
IF 3.777
Yingtao Ding,Qingxiang Wang,Feng Gao,Fei Gao
In this work, a dumbbell-shaped bi-acetylferrocene ethylenediamine complex, (AFc) 2 -en (AFc = acetylferrocene, en = ethylenediamine) was synthesized and its interaction with double-stranded DNA (dsDNA) was comprehensively studied by electrochemical methods on a gold electrode. The experimental results reveal that the highly electroactive (AFc) 2 -en complex can interact with dsDNA via a specific bis-groove binding mode. The binding constant and binding site size between the two species were determined to be 3.7 × 10 5 L mol −1 and 4.2, respectively. The surface-based studies further show that (AFc) 2 -en presents the stronger electrochemical response on dsDNA modified electrode than on the single-stranded DNA (ssDNA) modified electrode, suggesting that the complex can well distinguish dsDNA from ssDNA. Based on this characteristic, the bi-acetylferrocene complex was utilized as an electrochemical hybridization indicator in a DNA biosensor for the detection of oligonucleotides from CaMV35S promoter gene. The specificity experiments indicate that the biosensor can well discriminate the complementary sequence from the base-mismatched and the non-complementary sequences. The complementary target sequence can be quantified over a wide range from 1.0 × 10 −14 mol L −1 to 1.0 × 10 −6 mol L −1 with a detection limit of 2.0 × 10 −15 mol L −1 .
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ANALYTICAL CHEMISTRY
·
2014
·
IF 5.825
Lihua Zhang,Hua Liu,Yong Shao,Clement Lin,Huan Jia,Gang Chen,Danzhou Yang,Ying Wang
Aptamers, that exist naturally in living cells as functional elements and can switch nonfluorescent natural targets to fluorophores, are very useful in developing highly sensitive and selective biosensors and screening functional agents. This work demonstrates that human telomeric G-quadruplex (HTG) can serve as a potential fluorophore-switching aptamer (FSA) to target a natural isoquinoline alkaloid. We found that, among the G-quadruplexes studied here and the various structurally similar alkaloids including epiberberine (EPI), berberine (BER), palmatine (PAL), jatrorrhizine (JAT), coptisine (COP), worenine (WOR), sanguinarine (SAN), chelerythrine (CHE), and nitidine (NIT), only the HTG DNA, especially with a 5′-TA-3′ residue at the 5′ end of the G-quadruplex tetrad (5′-TAG3(TTAG3)3-3′, TA[Q]) as the minimal sequence, is the most efficient FSA to selectively light up the EPI fluorescence. Compared to the 5′ end flanking sequences, the 3′ end flanking sequences of the tetrad contribute significantly less to the recognition of EPI. The binding affinity of EPI to TA[Q] (Kd = 37 nM) is at least 20 times tighter than those of the other alkaloids. The steady-state absorption, steady-state/time-resolved fluorescence, and NMR studies demonstrate that EPI most likely interact with the 5′ end flanking sequence substructure beyond the core [Q] and the G-quadruplex tetrad in a much more specific manner than the other alkaloids. The highly selective and tight binding of EPI with the FSA and significantly enhanced fluorescence suggest the potential development of a selective EPI sensor (detection limit of 10 nM). More importantly, EPI, as the brightest FSA emitter among the alkaloids, can also serve as an efficient conformation probe for HTG DNA and discriminate the DNA G-quadruplex from the RNA counterpart. Furthermore, EPI can bind stoichiometrically to each G-quadruplex unit of long HTG DNA multimer with the most significant fluorescence enhancement, which has not been achieved by the previously reported probes. Our work suggests the potential use of EPI as a bioimaging probe and a therapeutic DNA binder.
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ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE
·
2014
·Vol. 640
·期 11
·2238-2246
·
IF 1.251
Peng Li,Mao-Fei Niu,Mei-Ju Niu,Min Hong
Four transition metal complexes, namely Cu 2 Cl 2 (HL 1 ) 2 ( 1 ), Co(HL 1 ) 2 ( 2 ), Cu(HL 2 ) 2 ( 3 ), Co(HL 2 ) 3 ( 4 ) {H 2 L 1 = 2-{[2-(2-hydroxy-ethoxy)-ethylimino]-methyl}-phenol, H 2 L 2 = 3-{[2-(2-hydroxy-ethoxy)-ethylimino]-methyl}-naphthalen-2-ol} were synthesized and characterized by single-crystal X-ray diffraction, IR spectroscopy, and elemental analysis. The interactions of these copper and cobalt complexes with calf thymus DNA (CT-DNA) were studied by absorption and emission titration spectroscopic methods. The interactions of these copper and cobalt complexes with bovine serum albumin (BSA) were also investigated using fluorescence, UV/Vis, and synchronous fluorescence spectroscopy. Further, the in vitro cytotoxic effect of the complexes are examined on human colon carcinoma cell lines (HCT-116) and human lung carcinoma cell line (A549).
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2016
·Vol. 20
·期 5
·1263-1270
·
IF 2.327
Wang Lili,Yu Feiyue,Wang Fang,Chen Zilin
We describe a method for detecting DNA methylation. It is based on direct oxidation of DNA bases at a glassy carbon electrode (GCE) modified with film of a multiwalled carbon nanotube-β-cyclodextrin composite. This nano-structured film causes a strong enhancement on the oxidation current of DNA bases due to its large effective surface area and extraordinary electronic properties. Well-defined peaks were obtained as a result of electro-oxidation of guanine (at 0.67 V), adenine (at 0.92 V), thymine (at 1.11 V), cytosine (at 1.26 V), and 5-methylcytosine (at 1.13 V; all data vs. saturated calomel electrode (SCE)). The potential difference between 5-methylcytosine and cytosine (130 mV) is large enough to enable reliable simultaneous determination and analysis. The interference by thymine can be eliminated by following the principle of complementary pairing between purine and pyrimidine bases in DNA. The modified electrode was successfully applied to the evaluation of 5-methylcytosine in a fish sperm DNA, the methylation level of cytosine was found to be 7.47 %, and the analysis process took less than 1 h.
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MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 3
·951-959
·
IF 4.58
Zhang Yuhuan,Wang Yanru,Zhu Wenxin,Wang Jing,Yue Xiaoyue,Liu Wei,Zhang Daohong,Wang Jianlong
The capability of multi-level logic operations in biological diagnosis is far from being fully realized. In particular, the simplification of logic judgments to realize rapid, on-site complex diagnoses is still a key issue in bioelectronics. The authors here describe the construction of a colorimetric multilevel DNA circuit that incorporates two IMPLY gates and a sample extraction technology (SET) with anti-BPA aptamer as a co-recognizing element and the color of gold nanoparticles as the output signal. The circuit can perform multi-bioanalysis of the plasticizers and hormone mimetics bisphenol A (BPA) and bisphenol S (BPS). The assay is based on the finding that the aptamer against BPA can recognize both BPA and BPS. This DNA logic detection system is simple, fast, sensitive and selective because of the utilization of a co-recognition element which decreased input numbers. This, in turn, cascades multiplex Boolean logic gates and simplifies the diagnostic challenge. Both BPA and BPS can be determined by photometry at 620 nm, the limit of detection being 1.5 ng⋅mL −1 for BPA and 1.3 ng⋅mL −1 for BPS. Application to (spiked) water samples shows good analytical performance in terms of recovery (95.4 to 106.8%), linear range (4.4 to 66 ng⋅mL −1 for BPA, 2.5 to 75 ng⋅mL −1 for BPS) and correlation coefficients (>0.98). In our perception, the method represents a simple route for the fabrication of colorimetric multi-level DNA circuits. It may shorten the gap between bioelectronic logic circuits and high throughput real sample analysis.
Illustration of sample extraction treatments (SET) of bisphenol A (BPA) and bisphenol S (BPS) and the three-input IMPLY1-IMPLY2 logic operation with BPA, BPS and anti-BPA aptamer as inputs.
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ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Panpan Gai,Chengcheng Gu,Ting Hou,Feng Li
Herein, we reported a novel ultrasensitive one-compartment enzyme biofuel cells (EBFCs)-based self-powered aptasensing platform for antibiotic residue detection. By taking full advantage of the unique features of both EBFCs-based self-powered sensors and aptamers, the as-proposed aptasensing platform has the merits of simple instrumentation, anti-interference ability, high selectivity, and low cost. In this study, DNA bioconjugate, i.e., SiO2@gold nanoparticles–complementary strand of aptamer (SiO2@AuNPs–csDNA), was elaborately designed and played a key role in blocking the mass transport of glucose to the bioanode. While in the presence of the target antibiotic, SiO2@AuNPs–csDNA bioconjugate broke away from the bioanode due to the aptamer recognition of the target. Without the blocking of glucose by the DNA bioconjugate, a significantly elevated open circuit voltage of the EBFCs-based aptasensor was obtained, whose amplitude was dependent on the antibiotic concentration. In addition, this proposed aptasensor was the first reported self-powered aptasensing platform for antibiotic determination and featured high sensitivity owing to the elaborate design of the DNA bioconjugate modified bioanode of EBFC, which was superior to those previously reported in the literature. Furthermore, due to the anti-interference ability and the excellent selectivity of the aptasensor, no special sample pretreatment was needed for the detection of antibiotics in milk samples. Therefore, the proposed EBFCs-based self-powered aptasensor has a great promise to be applied as a powerful tool for on-site assay in the field of food safety.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Yu Zou,Wanshan Wang,Fengyin Duan,Wen Yun,Hong Chen,Yiping Li,Lizhu Yang
An ultrasensitive fluorescence detection strategy of chloramphenicol (CAP) was developed based on product catalysis of tetrahedral DNA framework (TDF) and fluorescent quenching of MIL-101(Fe). The product was used to catalyze the reaction. As the concentration of catalyst increased, the reaction time was significantly shorted to 21 min which was much shorter than other isothermal amplification technologies. Moreover, the multiple fluorophores of TDF and high efficient quenching ability of MIL-101(Fe) provided better performance with a linear range for CAP detecting from 1.6 pM to 80 pM and the limit of detection (LOD) as low as 0.67 pM. In addition, it also demonstrated good specificity and resistance to interference from other related antibiotics. Importantly, this strategy exhibited satisfactory relative standard deviation and recovery results for practical application, exhibiting a favorable application prospect in CAP analysis.
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ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Lanlan Chen,Jingjing Lai,Siqi Dong,Wenjun Liu,Ximei Zhang,Huanghao Yang
Evaluating tumor radiosensitivity is beneficial for the prediction of treatment efficacy, customization of treatment plans, and minimization of side effects. Tracking the mitochondrial DNA (mtDNA) repair process helps to assess tumor radiosensitivity as mtDNA repair determines the fate of the cell under radiation-induced mtDNA damage. However, current probes developed to monitor levels of DNA repair enzymes suffered from complex synthesis, uncontrollable preparation, limited tumor selectivity, and poor organelle-targeting ability. Especially, the correlation between mtDNA repair activity and inherent radiosensitivity of tumors has not yet been explored. Here, we present a mitochondria-targeted DNA-based nanoprobe (TPP-Apt-tFNA) for in situ monitoring of the activity of the mtDNA repair enzyme and evaluating tumor radiosensitivity. TPP-Apt-tFNA consists of a DNA tetrahedral framework precisely modified with three functional modules on each of the three vertexes, that is, the tumor cell-targeting aptamer, the mitochondrion-targeting moiety, and the apurinic/apyrimidinic endonuclease 1 (APE1)-responsive molecule beacon. Once selectively internalized by tumor cells, the nanoprobe targeted the mitochondrion and specifically recognized APE1 to activate fluorescence, allowing the observation of mtDNA repair activity. The nanoprobe showed elevated APE1 levels in the mitochondria of tumor cells under oxidative stress. Moreover, the nanoprobe enabled the illumination of different levels of APE1-mediated mtDNA repair activity in different cell cycle phases. Furthermore, using the nanoprobe in vitro and in vivo, we found that tumor cells with high activity of mtDNA repair, which allowed them to recover from radiation-induced mtDNA lesions, had low sensitivity to radiation and an unsatisfactory radiotherapy outcome. Our work provides a new imaging tool for exploring the roles of mtDNA repair activity in diverse biological processes and for guiding tumor radiation treatment.
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NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 6
·4714-4718
·
IF 3.201
Yu Ling,Xiao Fang Zhang,Xiao Hui Chen,Li Liu,Xiao Hu Wang,De Shou Wang,Nian Bing Li,Hong Qun Luo
Sensitive detection of disease-related DNA is of great significance for early and accurate diagnosis and therapy. To achieve this aim, we constructed a dual-cycling amplification biosensor for target oligonucleotide detection. Based on the toehold-mediated strand displacement reaction (TSDR), target DNA competitively hybridized with the assistant DNA which previously formed a duplex complex with the trigger DNA, and the trigger DNA was released. Exonuclease III (Exo III) would specially hydrolyze the assistant DNA in the new duplex DNA to free the target DNA for a new cycle. Similarly, based on the TSDR and Exo III assisted amplification, the released trigger DNA would repeatedly open the G-quadruplex hairpins and promote the generation of numerous G-quadruplex sequences. Then, the fluorescence intensity of Thioflavin T (ThT) would present apparent enhancement due to the formation of the G-quadruplex/ThT complex. The commercially available ThT is economical and of low toxicity. More importantly, owing to its satisfactory water solubility, ThT effectively avoids the introduction of organic solvents. There was no need for expensive modification through the whole process. The proposed method displayed high distinction efficiency towards target DNA against mismatched DNA. Additionally, the linear range of this strategy was 0.2–10 nM, and the detection limit was 0.04 nM. Besides, this sensor can be applied in human serum with satisfactory results.
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危险品化学品经营许可证（带存储）