FOOD CONTROL
·
2025
·
IF 6.3
Xishun Peng,Zhongchen Bai,Caizhen Zu,Yuxue He,Qi Zhao
The presence of thiram residues on fruit peels posed a significant risk to human health. Here, we prepared an electrochemical surface-enhanced Raman spectroscopy (EC-SERS) sensor of SPE/RGO/Au@Ag flexible film to detect thiram residues on fruit peel. The surface of this film was evenly covered with Au@Ag nanoparticles, which allowed it to specifically identify thiram residues through the formation of Ag-S bond. In addition, an electrochemical method was used to modulate the energy level matching between the SERS film and the thiram molecules to improve the chemical enhancement effect of the SERS. The results indicated that the charge transfer degree between the SERS film and the thiram molecules was up to 0.875. The SPE/RGO/Au@Ag film achieved a limit of detection (LOD) of 2.1 pM at -1.3 V. Owing to its excellent SERS performance, the SPE/RGO/Au@Ag film could successfully recognize the thiram residues on apple and orange peels, with recoveries of 91% to 107% and from 89% to 102%, respectively. The flexible EC-SERS sensor offers considerable promise for sensitively recognizing thiram residues on fruit peels.
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Xinyu Lan,Guojun Weng,Jianjun Li,Jian Zhu,Junwu Zhao
Surface metal deposition is an important method for tuning the optical property of plasmonic nanoparticles. In this work, we tried to deposit Cu shell on the surface of gold nanorods (Au NRs) by dihydronicotinamide adenine dinucleotide (NADH)-mediated reaction. Cu 2+ were reduced to Cu 0 through the gold-catalyzed oxidization of NADH. The deposition of Cu shell onto the Au NRs surface resulted in the obvious red-shift of longitudinal localized surface plasmon resonance peak. And the red-shift of longitudinal peak increased with the concentrations of NADH and Cu 2+ . The TEM images showed that a transparent layer structure with a different contrast around Au NRs was clearly displayed. And this transparent layer structure with the low contrast was generally considered to be pure Cu shell. However, the results from energy dispersive spectrometer (EDS) and X-ray photoelectron spectroscopy (XPS) indicated that the transparent layer was not just pure Cu composition, but contained NADH/NAD + and Cu 2+ components. This grown complex dielectric shell resulted in the reduced surface enhanced Raman scattering (SERS) activity of Au NRs. Based on the decreased SERS intensity, we demonstrated a SERS method for Cu 2+ detection using R6G as Raman reporter. The decreased SERS intensity had a linear correlation with the concentration of Cu 2+ in the range of 10 −6 –10 −2 M, with a detection limit of 3.89 × 10 −6 M. This study provides new insights about Cu deposition on Au NRs, which will further promote the spectral sensing applications with gold-catalyzed Cu 2+ reduction on plasmonic nanoparticles by NADH.
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高影响因子
TALANTA
·
2025
·
IF 6.1
Wei Li,Xinrui Wang,Mingze Zhu,Xin Huang,Pacifique Hirwa Umutoni,Ting-Hsuan Chen,Jian Lu,Shih-Chi Chen,Guanming Tan,Bryan P. Yan,Bee Luan Khoo
Chronic inflammation remains a major global health concern, necessitating the development of advanced tools for continuous, precise monitoring. This study introduces a novel, clinically impactful triple-mode probe that integrates colorimetric, fluorescence, and surface-enhanced Raman scattering (SERS) signals, offering unparalleled multimodal detection capabilities. The probe's design leverages europium chelate-doped polystyrene nanoparticles (ECNPs), ensuring minimal signal cross-interference through a long Stokes shift. A key innovation is the development of a multimodal mapping algorithm that seamlessly integrates these optical signals, providing a sensitive and robust platform for biomarker detection with broad dynamic ranges. The probe's clinical relevance is demonstrated by its application in lateral flow assays (LFAs) for detecting C-reactive protein (CRP) levels, achieving a detection limit of 6.31 ng/mL and dynamic ranges from 23.13 to 2000 ng/mL, significantly outperforming single-mode detection methods. In clinical validation using urine samples from 31 patients, the triple-mode LFA showed excellent correlation (0.9377) and agreement (93.55%) with gold standard enzyme-linked immunosorbent assay (ELISA) results. This demonstrates that the proposed multimodal platform offers a highly sensitive, cost-effective, and versatile tool for monitoring inflammation and other disease biomarkers, with substantial potential for clinical applications in diagnostics and disease management.
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高影响因子
Sensors & Diagnostics
·
2023
·Vol. 2
·期 5
·1292-1301
·
IF 0
Yu Lu,Ruihua Fei,Jiahe Zhang,Geng Zhu,Xiufang Mo,Yu Wan,Yan Huang,Qingjiang Sun,Dianhuai Meng,Xiangwei Zhao
The clinical symptoms of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), are similar to those of influenza A infection. Therefore, accurate diagnosis of SARS-CoV-2 and influenza A in the early infection stage is essential for appropriate clinical treatment. In this work, a vertical flow assay (VFA) device based on Au@Ag@Au core–shell SERS nanotags and anodic aluminum oxide (AAO) was proposed to diagnose and distinguish between SARS-CoV-2 and influenza A at the same time. Antibodies paired with SARS-CoV-2 and influenza A were immobilized simultaneously on the AAO. In the presence of SARS-CoV-2 or influenza A antigens, sandwich immune-complexes were formed on the AAO and the corresponding SERS signals of nanotags could be detected. The whole detection process took only about 15 minutes, taking comparable or even shorter time than reported POCT antigen assays. The detection limit reached 0.47 pg ml−1 for SARS-CoV-2 nucleocapsid protein (NP) and 0.62 pg ml−1 for influenza A antigen. The clinical feasibility of the proposed VFA device was validated by testing clinical throat swab samples (3 SARS-CoV-2 positive samples and 17 negative samples). Real sample analysis indicated a well-distinguished positive/negative sample group. Conceptually, this VFA device is a potential POCT diagnosis tool for determining if a suspected patient is infected with SARS-CoV-2 or influenza A.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2018
·
IF 3.412
Nan Yang,Ting-Ting You,Yu-Kun Gao,Chen-Meng Zhang,Peng-Gang Yin
Surface enhanced Raman scattering (SERS) has been widely used in detection of food safety due to the nondestructive examination property. Here, we reported a flexible SERS film based on a polymer-immobilized gold nanorod polymer metafilm. Polystyrene–polyisoprene–polystyrene (SIS), a transparent and flexible, along with having excellent elasticity, polymer, was chosen as the main support of gold nanorods. A simple phase transfer progress was adopted to mix the gold nanorods with the polymer, which can further be used in most water-insoluble polymers. The SERS film performed satisfactorily while being tested in a series of standard Raman probes, like crystal violet (CV) and malachite green (MG). Moreover, the excellent reproducibility and elastic properties make the film a promising substrate in practical detection. Hence, the MG detection on the fish surface and trace thiram detection on orange pericarp were inspected with detection results of 1 × 10–10 and 1 × 10–6 M, which were below the demand of the National standard of China, exactly matching the realistic application requirements.
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高影响因子
Small
·
2024
·Vol. 21
·期 6
·2409806
·
IF 13
Ruiyuan Zhang,Yu Guo,Chen Huang,Jixiang Fang
The heterogeneity of extracellular vesicles (EVs) surface information represents different functions, which is neglected in previous studies. In this study, a label-free SERS analysis approach is demonstrated to study fundamental EV biological and physical information heterogeneity by matching specific sizes of nano-enhanced particles. This strategy reveals informative, comprehensive, and high-quality SERS spectra of the overall exosome surface, and effectively circumvents the key information loss caused by the spatial resistance of NPs binding to the 293 exosomes' concave structure. The classification of normal and cancerous cell-derived exosomes by PCA method, the accuracy is improved from 91.2% to 95.1% by optimizing sizes of nano-enhanced particles. In addition, stem cell-derived EVs of diverse sizes and morphologies similarly show acuity of spectrum variation to NPs size, which is conductive to qualitative studies. This new strategy will offer a widened in-depth understanding of the surface information, size, and morphology of EVs, which can be applied to the study of biological functions.
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高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 7
·1-9
·
IF 5.7
Wang Wenxi,Ruan Shuyan,Su Zhixiong,Xu Peipei,Chen Yujia,Lin Zheng,Chen Jingbo,Lu Yudong
A novel S-CNF-based nanocomposite was created using sulfonated cellulose nanofiber (S-CNF) to enable the detection of NADH in serum by surface-enhanced Raman spectroscopy (SERS). The numerous hydroxyl and sulfonic acid groups on the S-CNF surface absorbed silver ions and converted them to silver seeds, which formed the load fulcrum. After adding a reducing agent, silver nanoparticles (Ag NPs) were firmly adhered to the S-CNF surface to form stable 1D “hot spots.” The S-CNF-Ag NP substrate demonstrated outstanding SERS performance, including good uniformity with an RSD of 6.88% and an enhancement factor (EF) of 1.23 × 10 7 . Owing to the anionic charge repulsion effect, the S-CNF-Ag NP substrate still maintains remarkable dispersion stability after 12 months of preservation. Finally, S-CNF-Ag NPs’ surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH). The results showed that the detection limit (LOD) of NADH was 0.75 μM; a good linear relationship ( R 2 = 0.993) was established in the concentration range 10 −6 – 10 −2 M. The SERS nanoprobe enabled rapid detection of NADH in human serum without any complicated sample pretreatment and provides a new potential to detect biomarkers. Graphical
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 4
·1-12
·
IF 5.3
Ruan Shuyan,Wang Wenxi,Qiu Liting,Yan Xin,Peng Zhihua,Zhu Huina,Liu Yunzhen,Lu Yudong,You Ruiyun
A simple and cost-effective fabrication method of gold nanorods (AuNRs) nanoparticles hybridized with polyvinyl alcohol hydrogel (AuNR/PVA) for SERS substrate is described. The AuNR/PVA achieves the control of inter-particle nanogap by modulating the density of gold nanorods, and inter-particle nanogap by the spatial deformation of the hydrogel, and the reduction of the gap between the AuNRs deposited on hydrogel makes the SERS enhancement. In addition, the AuNR/PVA substrate maintains high SERS activity after more than 100 cycles of bending and storage in air for 30 days, and the substrate possesses high sensitivity and high reproducibility. Combining a flexible and transparent surface-enhanced Raman spectroscopy (SERS) substrate for in situ detection with a small portable Raman can be applied to scenarios such as environmental detection and hazardous materials detection. The substrate showed excellent SERS activity against malachite green (MG) and crystal violet (CV) with limits of detection of 1.18 × 10 −13 M and 7.17 × 10 −12 M, respectively. The usability of the proposed SERS substrate was demonstrated by detecting the above contaminants in aquatic water. This work not only utilizes a cost-effective method for mass production but also provides a reliable and convenient platform for the preparation of other noble metal flexible substrates. Graphical
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Li Sun,Zheng Ye,Yuan Zhi,Hua Zhang,Binlin Dou,Yuejin Shan,Jian Chen
In this study, a prospective strategy on seed-mediated growth in vacuum is provided and used to prepare flexible silver nanofilms (AC 0–3 NFs) with high purity, crystallinity and SERS performance. Noteworthy, AC 2 NFs surface is uniformly covered with silver nanoparticles (Ag NPs), following the appearance of numerous nano-gaps between Ag NPs. Through the finite-difference time-domain (FDTD) simulation, it is found that the ultra-small nano-gaps less than 5 nm can generate abundant “hot spots” to amplify the Raman signal. Therefore, the above AC 2 NFs as SERS substrates exhibit superior activity for the SERS detection of thiram in tomato juice, with the limit of detection (LOD) of 10 −15 M and the enhancement factor (EF) of 3.7 × 10 11 . Meanwhile, AC 2 NFs show outstanding reproducibility and stability during the SERS detection. Additionally, the calibration curve is established, by which the thiram (10 −7 to 10 −15 M) can be quantitatively detected. Clearly, the as-prepared flexible AC 2 NFs have great potential in the SERS detection and analysis.
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高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Abubakkar Khan,Jiawei Shen,Aiman Zahid,Cancan Hu,Xuhua Xu,Xiaoyu Cheng
Label-free surface-enhanced Raman scattering (SERS) exhibits potential for liquid biopsies but requires further developments with clinical translations. Here, we demonstrate a label-free SERS immunoassay for quantifying amyloid beta (Aβ) using a clinical cohort of Alzheimer’s disease (AD), featuring robustness, speed, and economical and clinical validity. This was demonstrated using a gap-mode SERS with aptamer-modified mesoporous gold (mesoAu) and gold-coated magnetic nanoparticles (Au@MNPs). The mesoAu substrate was prepared by electrodeposition with pore sizes tuned between 30 and 70 nm, and the Au@MNPs were synthesized by hydrothermal methods. A self-assembled monolayer with a mixture of polyethylene glycol (PEG) and DNA aptamers was modified onto the surface of gold, reducing nonspecific adsorptions and enabling target recognition to allow measurements in complex media. Using the intrinsic SERS fingerprints of phenylalanine (Phe), which are orthogonal to the DNA aptamer, it is shown that this assay is capable of quantifying Aβ42 in cerebrospinal fluid (CSF) in a label-free manner, with a limit of detection (LoD) of ∼0.15 pM, an assay time of ∼20 min, and cost less than ∼$10. The assay was further demonstrated using a clinical cohort containing 30 cerebral spinal fluid (CSF) samples of AD, mild cognitive impairment (MCI), and controls. The results showed clinical validity in differentiating the groups. Due to the simplicity and robustness of the assay, it is expected that this work will pave the way for label-free SERS bioassays for early diagnosis of major diseases using molecular biomarkers.
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高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 193
·期 1
·37
·
IF 5.3
Yao Yunfeng,Chen Shuyang,Hu Lingquan,Cheng Cheng,Wu Long,Liu Xing,Zhang Sihang,Jiang Shouxiang
AuNPs-decorated electrospun cellulose acetate nanofiber (AuNPs@CA-NF) SERS substrates were fabricated using a straightforward magnetron sputtering approach. Magnetron sputtering enables not only the uniform deposition of gold nanoparticles on electrospun nanofiber membranes, but also offers advantages such as high speed, scalability for mass production, and the absence of chemical reagents. Consequently, the optimized AuNPs@CA-NF SERS substrate achieved a detection limit of 10 − 11 M for 4-mercaptobenzoic acid (4-MBA), with reliable signal uniformity (RSD = 9.5%) and strong storage stability. The excellent linear relationship between SERS intensity and logarithmic concentrations shows great potential for rapid and quantitative determination of hazardous molecules. Moreover, thanks to the excellent flexibility of the electrospun polymer substrate, the SERS substrate can also detect pesticide residues on irregular fruit surfaces through a simple “adhere-and-read” method. The flexible electrospun polymer SERS substrate achieves a detection limit of 10 − 7 M for thiram, underscoring its strong potential for rapid and ultra-trace monitoring of hazardous substances in food safety.
Section
Graphical abstract
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高影响因子
APPLIED SURFACE SCIENCE
·
2012
·
IF 2.103
Zhongbo Li,Guowen Meng,Ting Liang,Zhuo Zhang,Xiaoguang Zhu
We report a facile low-cost synthetic approach to large-scale Ag nanosheet-assembled films with a high density of uniformly distributed sub-10 nm gaps between the adjacent nanosheets on Si substrates via galvanic cell reactions. The distribution density of Ag nanosheets on substrates could be tailored by tuning the duration of the HF-etching and the concentration of citric acid in the solution. Furthermore, in conjunction with a conventional photolithography, highly uniform patterned Ag nanosheet-assembled structures with different morphologies can be achieved on Si substrates via galvanic-cell-induced growth. By using rhodamine 6G as a standard test molecule, the large-scale Ag nanosheet-assembled films exhibit highly active and homogenous surface-enhanced Raman scattering (SERS) effect and also show promising potentials as reliable SERS substrates for rapid detection of trace polychlorinated biphenyls (PCBs).
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危险品化学品经营许可证（带存储）