Nature Microbiology
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2020
·Vol. 5
·期 8
·1040-1050
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IF 15.54
Song Meirong,Liu Yuan,Huang Xiaoyong,Ding Shuangyang,Wang Yang,Shen Jianzhong,Zhu Kui
The rapid emergence and dissemination of multidrug-resistant (MDR) bacterial pathogens pose a serious threat to global healthcare. One particular concern is the carbapenem-resistant Enterobacteriaceae (CRE), a group of Gram-negative bacteria that have evolved resistance to all or nearly all available antibiotics. Coupled with the fact of barren antibiotic development pipeline nowadays, a critical approach is to revitalize existing antibiotics using antibiotic adjuvants. We found a short linear antibacterial peptide (SLAP)-S25 carrying four non-natural amino acids of 2,4-diaminobutanoic acid (Dab), which solely showed weak antibacterial activity but boosted the efficacy of antibiotics covering all major classes, including cefepime, colistin, ofloxacin, rifampicin, tetracycline and vancomycin, against MDR Gram-negative pathogens. Mechanistic studies showed that SLAP-S25 triggers membrane damage by binding to both lipopolysaccharide (LPS) in the outer membrane and phosphatidylglycerol (PG) in bacterial cytoplasmic membrane, to potentiate antibiotic efficacy through collaborative strategies. Lastly, SLAP-S25 effectively enhanced the activity of colistin against MDR Escherichia coli -associated infections in three animal models. Our findings provide a potential therapeutic option using existing antibiotics in combination with broad-spectrum antibiotic adjuvants, to address the prevalent infections caused by MDR Gram-negative pathogens worldwide.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
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2020
·Vol. 36
·期 6
·1279-1283
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IF 1.063
Gao Liguo,Yan Yeling,Li Yang,Ma Tingli
To reduce the quadratic scaling of the series resistance( R s ) and sheet resistance( R st ) of the devices, physical isolation of the large area devices into small pieces has been proven to be a reliable and cost-efficient patterning technique. In this paper, we got an interesting result that the physical isolation did not show obvious effect on the photovoltaic performance of perovskite solar cells(PSCs) when fixing the active area. Three different isolation types, unetched ITO, etched ITO, and laser etching whole devices, have been induced to investigate the physical isolation roles. The results show that the electrons and holes could be collected efficiently in active area for all the isolation types. The proposed mechanism illustrates that the nonradiative recombination and recombination of electrons and hole in inactive area do not influence the performance of devices. This work may open a new way for the commercialization of PSCs by reducing the complex process and the etching costs.
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EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2020
·Vol. 246
·期 7
·1483-1494
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IF 2.366
Zhu Jiangxiong,Du Mengke,Wu Meirong,Yue Pengxiang,Yang Xiufang,Wei Xinlin,Wang Yuanfeng
In this study, two novel mixed ACE-inhibiting peptides, namely Se-TAPep 1 and TAPep 1, were prepared and purified from selenium-enriched and common alkali-soluble proteins using a series of process, and then its physicochemical properties and oligopeptide sequence were analyzed. The results showed that Se-TAPep 1 and TAPep 1 could be successfully separated, but their ACE inhibitory activities were different (IC50 values were 13.39 mg/mL and 23.56 mg/mL, respectively), this may be related to the difference in selenium content. Further analysis of physicochemical properties showed that the protein or peptide fractions contained their characteristic absorption peaks and contained aromatic amino acids, while the apparent morphology was different, the presence of selenium could change the apparent morphology of protein or polypeptide fractions, arranging of selenium-enriched fractions more irregular. The amino acid composition analysis showed that the Pro content of the selenium-enriched fractions was higher than that of the ordinary component, whereas the oligopeptide sequence analysis showed that both LQPSLGFP derived from Se-TAPep 1 and AETGEIKGHY derived from TAPep 1 contained some characteristics of highly active ACE-inhibiting peptides, but the characteristics of LQPSLGFP were more conspicuous, which may be related to its molecular weight and source of mixed peptides. These findings indicated that the selenium-enriched tea mixed peptides had better potential uses than ordinary tea mixed peptides in the preparation of functional food.
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FIBERS AND POLYMERS
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2020
·Vol. 21
·期 5
·1126-1133
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IF 1.797
Meng Yang,Zhang Bin,Su Juanjuan,Han Jian
Inspired by the layered structure and oriented materials, this study exhibits a reinforcement and antibacterial composite consisted by polytetrafluoroethylene/glass fiber (PTFE/GF) composites and QACMt. The QACMt used as an organically modified layered particle is fabricated by a method that montmorillonite (Mt) is modified with quaternary ammonium compounds (QAC). Owing to the composites have a unique layered structure, it was considered as an ideal reinforcement to strengthen polymer. Importantly, the tensile strength and toughness of PTFE/GF composites are achieved the maximal value of 25.5 MPa and 18.1 MJ/m 3 , respectively. Additionally, the QACMt exhibits good antibacterial activities against S. aureus and E. coli. This work guides a layered structure to achieve exceptionally high strength and toughness, antibacterial properties.
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Journal of Analysis and Testing
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2020
·Vol. 4
·期 4
·249-256
·
IF 0
Zuo Jiali,Cai Runxian,An Yanpeng,Tang Huiru
Abstract Stereoisomeric hexoses are present in almost all biological organisms in the forms of aldoses and ketoses, with diverse physiological and pathophysiological functions. Accurate and simultaneous quantification is vital for understanding their functions individually. However, such analysis remains challenging owing to their highly similar behavior in chromatography and mass spectrometry. By combining the pre-column 3-nitrophenylhydrazine derivatization and ultrahigh performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), here, we developed a method for simultaneous quantification of five important stereoisomeric hexoses including D-glucose, D-galactose, D-mannose, D-fructose and L-sorbose representing both aldoses and ketoses. The method achieved baseline-separation for all these five derivatized hexoses chromatographically and had high sensitivity (LOD, femtomole on column), excellent linearity ( R 2 > 0.995) and efficiency with stable-isotope dilution. With this method, we further quantified these hexoses in nine biological matrices including human biofluids (serum, urine and saliva), human cells, human and mouse feces, rat liver tissue, mung-bean seeds and peach pulp. The results provided quantitative data for these hexoses in multiple biological samples and showed significant concentration diversity for these hexoses in different biological samples, which demonstrated the applicability of the method for simultaneous quantification of these hexose phenotypes of biological systems. Graphic Abstract
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JOURNAL OF POLYMER RESEARCH
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2020
·Vol. 27
·期 8
·1-12
·
IF 2.426
Ji Bingqiang,Wu Yeping,Zhang Ping,Zhao Xiuli
Epoxy resin is widely used in various industrial fields due to its good adhesion, corrosion resistance and the like, but its thermal conductivity is low and it is difficult to meet the heat dissipation requirements. In order to obtain epoxy composites with high thermal conductivity and excellent electrical insulation, an effective method for functionalizing boron nitride (BN) and multi-walled carbon nanotubes (CNTs) is proposed. The BN and CNTs were first ultrasonically mixed, and then functionalized by a simple dip coating procedure with polydopamine (PDA) coating (named as m(BN/CNT)). Fourier transform infrared spectroscopy, thermogravimetric analysis and X-ray photoelectron spectroscopy analysis demonstrated the successful functionalization of PDA on BN and CNTs surfaces. Adding 20 vol% m(BN 10 /CNT 1 ) increased the thermal conductivity of the epoxy composite by 231% compared to pure epoxy matrix, while still maintained a volume resistivity above 10 14 Ω·cm. Dynamic mechanical analysis and scanning electron microscopy showed that PDA functionalization improved the interfacial interaction between BN, CNTs and the matrix, thus ensuring the improved thermal and mechanical properties.
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BIOTECHNOLOGY LETTERS
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2020
·Vol. 42
·期 10
·2049-2058
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IF 1.977
Song Lingyu,Wang Yan,Zhen Yunhuan,Li Dengke,He Xidong,Yang Hong,Zhang Huayang,Liu Qi
Cancer metastasis is the primary cause of death in patients diagnosed with colorectal cancer. Piperine, an active nontoxic ingredient in pepper, has potent anti-inflammatory and anti-cancer properties. However, little is known about the anti-migratory and anti-invasive effects of piperine on colorectal cancer. We demonstrated piperine inhibited the migration and invasion of colorectal cancer cells. Then, we found piperine reversed the biomarker expression of epithelial-to-mesenchymal transition (EMT), and suppressed the EMT regulator Snail. Furthermore, signal transducers and activators of transcription 3 (STAT3) was downregulated by piperine. Finally, STAT3 inhibitors were applied to observe the role of STAT3 in colorectal cancer migration, invasion and EMT. Collectively, piperine inhibits colorectal cancer migratory and invasive capacities through STAT3/Snail mediated EMT. Therefore, piperine could be applied as a possible therapeutic regimen for the prevention of colorectal cancer metastasis.
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MICROCHIMICA ACTA
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2020
·Vol. 187
·期 7
·1-8
·
IF 6.232
Li Nianlu,Li Ruohan,Song Yinshuang,Ma Long,Gao Cuiling,Li Lili,Cheng Shi-Bo,Zhang Xiaoli,Chen Jing,Zhan Jinhua
Carbonaceous shell-coated γ-Fe 2 O 3 nanoparticles (γ-Fe 2 O 3 @CNM) were synthesized from glucose caramelization and used as a novel magnetic solid-phase extraction medium for malachite green and crystal violet in environmental water. Malachite green and crystal violet were absorbed on to γ-Fe 2 O 3 @CNM by electrostatic and π-interactions. The morphologies, pore structures, surface functional groups, and magnetic properties of γ-Fe 2 O 3 @CNM were characterized by TEM, FTIR, hysteresis regression, Brunauer-Emmet-Teller analysis, zeta potential, XPS, and XRD. The magnetic solid-phase extraction procedure was optimized by extraction pH, absorption time, desorption solvent, and desorption time. The absorption capacities (q max values) for malachite green and crystal violet were 34.2 and 27.9 mg g −1 , respectively. After magnetic solid-phase extraction, malachite green and crystal violet were determined by LC-MS/MS. The analytical method was validated with a linear range of 0.02–20 ng mL −1 , enrichment factor of 25.8 and 25.4, method detection limit of 0.004 ng mL −1 , and intra-day precisions of 2.1% and 2.6% for malachite green and crystal violet, respectively. The relative recovery was found to be 73.4–101.5% for malachite green and 83.1–102.7% for crystal violet upon the application of the magnetic solid-phase extraction method to real water samples from lake, spring, sea, fishpond, and industrial waste.
Caramelized-carbon-coated magnetic nanoparticles are used as novel extraction medium based on electrostatic and π-interactions. It is porous, amphiphilic, electronegative, magnetically strong, and features abundant absorption site. These characteristics stimulate mass transfer and result in a useful MSPE method in environmental analysis.
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Nature Communications
·
2020
·Vol. 11
·期 1
·1-8
·
IF 12.121
Zhang Dao,Xiao Wenge,Liu Chang,Liu Xiaofeng,Ren Jinjun,Xu Beibei,Qiu Jianrong
The development of high-power white light-emitting diodes demands highly efficient and stable all-inorganic color converters. In this respect, phosphor-glass/ceramic composites show great promise as they could combine the merits of high quantum efficiency of phosphors and high chemical and thermal stabilities of glass/ceramic matrices. However, strong interfacial reaction between phosphors and matrices at high temperature results in quantum efficiency loss of the embedded phosphors, and traditional solutions rely on high-pressure consolidation techniques. Here we report the intrinsic inhibition of interfacial reaction by using silica glass rather than multicomponent glasses as the matrix. The embedment of phosphors is achieved via a pressureless sintering method, rendering these color-tunable phosphor-glass composites not only accessible to three-dimensional printing technique, but also highly efficient (internal quantum efficiency >90.0%), thermally stable at 1200 °C and hydrothermally stable at 200 °C. Our results provide a facile and general strategy for developing all-inorganic functional composites.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2020
·Vol. 31
·期 13
·10489-10498
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IF 2.22
Wang Shitong,Wang Jiemei,Ji Xiang,Meng Jiaxi,Sui Yanwei,Wei Fuxiang,Qi Jiqiu,Meng Qingkun,Ren Yaojian,He Yezeng,Zhuang Dongdong
Metal hydroxide serving as a promising electrode material for the supercapacitors, has attracted considerable interests in the area of energy storage. However, the low cycling stability and electrical conductivity restrict its further application. Here, a kind of hollow-cubic Ni(OH) 2 /CuS 2 with excellent electrochemical performance has been prepared via a facile one-step sacrificial template method. The obtained Ni(OH) 2 /CuS 2 shows an outstanding specific capacitance of 1174 F g −1 at 1 A g −1 with 73.9% retention after 2000 charge–discharge cycles. Furthermore, the assembled asymmetric supercapacitors based on hollow-cubic Ni(OH) 2 /CuS 2 nanocomposite and reduced graphene oxide (RGO) exhibits a high specific energy density of 27.8 Wh kg −1 at the power density of 390 W kg −1 .
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 27
·7525-7533
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IF 3.637
Wu Huanan,Wang Shuokang,Li Sam Fong Yau,Bao Qi,Xu Qiyong
Detection of lead(II) (Pb 2+ ) ions in water is important for the protection of human health and environment. The growing demand for onsite detection still faces challenges for sensitive and easy-to-use methods. In this work, a novel surface plasmon resonance (SPR) biosensor based on GR-5 DNAzyme and gold nanoparticles (AuNPs) was developed. Thiolated DNAzyme was immobilized on the gold surface of the sensor chip followed by anchoring the substrate-functionalized AuNPs through the DNAzyme-substrate hybridization. The coupling between the localized surface plasmon (LSP) of AuNPs and the surface plasmon polaritons (SPP) on the gold sensor surface was used to improve the sensitivity. The substrate cleavage in the presence of Pb 2+ ions was catalyzed by DNAzyme, leading to the removal of AuNPs and the diminished LSP-SPP coupling. The optimal detection limit was 80 pM for the sensor fabricated with 1 μM DNAzyme, corresponding to two or three orders of magnitude lower than the toxicity levels of Pb 2+ in drinking water defined by WHO and USEPA. By tuning the surface coverage of DNAzyme, the sensitivity and dynamic range could be controlled. This sensor also featured high selectivity to Pb 2+ ions and simple detection procedure. Successful detection of Pb 2+ ions in groundwater indicates that this method has the prospect in the onsite detection of Pb 2+ ions in water. Given the variety of AuNPs and metal-specific DNAzymes, this detection strategy would lead to the development of more sensitive and versatile heavy metal sensors.
Graphical abstract
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 9
·1-8
·
IF 6.232
Guo Manli,Chi Jingtian,Li Yijing,Waterhouse Geoffrey I. N.,Ai Shiyun,Hou Juying,Li Xiangyang
Carbon dots and gold nanoclusters co-encapsulated by zeolitic imidazolate framework-8 (CDs/AuNCs@ZIF-8) have been obtained at room temperature. The composite has been applied to the ratiometric fluorescence determination of mercury(II). The composite shows fluorescence emission maxima at 440 and 640 nm under 360 nm excitation, due to the CDs and AuNCs, respectively (associated quantum yields were 18% and 17%, respectively). In the presence of Hg 2+ , the fluorescence at about 640 nm is quenched, while the fluorescence at about 440 nm is unaffected. The CDs/AuNCs@ZIF-8 composite allows the sensitive detection of Hg 2+ , with the fluorescence intensity ratio ( I 640 / I 440 ) decreasing linearly with Hg 2+ concentration over the range 3–30 nM. The fluorescence emission of the composite changes color from red to blue with increasing Hg 2+ under UV excitation, which can easily be discerned visually. This visual detection of Hg 2+ is due to the high fluorescence quantum yields of the CDs and AuNCs and the ~ 200 nm separation between the two emission maxima.
(A) Schematic diagram showing the operating principle of the determination for Hg(II). (B) Digital graph of the solutions in absence and presence of 30 nM Hg(II) under a portable UV lamp
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