SEPARATION AND PURIFICATION TECHNOLOGY
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2024
·
IF 8.1
Yanyan Xing,Mengke Tian,Ruoman Ji,Ting Wang,Xiaohong Hou
The immobilization of bovine serum albumin (BSA) with high efficiency, good reproducibility and stability for chiral separation has attracted much attention in the medicinal field. BSA was immobilized into MIL-101(Cr) by three ways for efficient chiral separation in this research. MIL-101(Cr)@BSA was firstly prepared by adsorption, NH 2 -MIL-101(Cr) ⊂ BSA by direct bonding, and NH 2 -MIL-101(Cr) ⊂ Glu ⊂ BSA by glutaraldehyde crosslinking bonding. Compared to the other two materials, NH 2 -MIL-101(Cr) ⊂ BSA as a new enantioselective separator, with the most BSA loading rate of 437.6 mg g −1 exhibited excellent chiral separation performances and succeeded in realizing a complete chiral separation of racemic 1–phenylethanol within 14 s. It was found that the NH 2 -MIL-101(Cr) ⊂ BSA was more strongly bound to R -1-phenylethanol and the ee value could reach 100 % when the material was added in three batches. The different interactions between BSA and racemates mainly including hydrogen bonding, hydrophobic interaction and π-π interaction were revealed by the molecular docking studies, especially hydrogen bonding, accounting for the observed selective chiral recognition capacity of racemic 1–phenylethanol of NH 2 -MIL-101(Cr) ⊂ BSA. Moreover, both of NH 2 -MIL-101(Cr) ⊂ BSA and NH 2 -MIL-101(Cr) ⊂ Glu ⊂ BSA showed excellent stability and reusability. This combination of theory and experiment provided a clearer basis for other researchers to have different requirements for protein immobilization, and provided new ideas and methods for the development of chiral selectors.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Lisong Xia,Lei Cheng,Wenni Xi,Xiliang Zhang,Xianyang Shi
The environmental fate and risks of mononitrophenols (mono-NPs), the simplest nitrophenols (NPs) often found in aquatic environments, are profoundly influenced by anaerobic bioreduction and co-existing electron shuttles (ESs), but little is known about the underlying mechanisms. Here, we elucidate the pathways of anaerobic mono-NPs bioreduction by Shewanella oneidensis MR-1 and assess the effect of model ESs on these processes. We found that all three mono-NPs isomers could be readily reduced to their corresponding aminophenols by S. oneidensis MR-1 under anaerobic conditions. CymA, a core component of the Mtr respiratory pathway, performs a dynamic role in these bioreduction, which is highly dependent on the bioreduction kinetics. The exogenous addition of quinones was found to accelerate the mono-NPs bioreduction through interactions with key outer-membrane proteins (e.g., OmcA and MtrC), and all these processes matched well to linear free energy relationships (LFERs). Surprisingly, adding riboflavin did not influence the bioreduction of all three mono-NPs isomers, which may be due to the contribution of OmcA and MtrC to these bioreduction processes and their downregulated expression. This study enhances our understanding of the environmental fate of mono-NPs and their bioconversion processes, providing valuable insights for the bioremediation of nitrophenol-contaminated sites.
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高影响因子
POLYMER COMPOSITES
·
2024
·Vol. 45
·期 10
·8770-8780
·
IF 4.8
Weixing Sun,Guixiang Liu,Xinpeng Fan,Shuangquan Liao
Improving the interfacial interaction between silica and rubber is always a key issue to be solved for silica-filled rubbers. In this paper, composites are prepared by an in-situ modification method using 1-ethylpyridinium bromide ([EPy]Br) as the raw material, and the effect of its dosage on the interfacial interactions and macroscopic properties of silica-filled natural rubber (NR) composites is investigated. The effect of [EPy]Br on silica dispersion and interfacial interaction between silica and NR is verified through rheological tests and calculations of glass layers. Compared with the original samples, composite performance is optimized when the usage amount is at 1phr which increases tensile strength by 5 MPa, enhances abrasion resistance by 24%, and shortens optimum curing time in half. This study is expected to provide a new strategy for improving the processability of silica-containing composites while reducing energy consumption. Highlights [EPy]Br enhances the interaction between rubber and filler while modifying the surface of silica. Increasing the dosage of [EPy]Br leads to a reduction in the Payne effect. The addition of [EPy]Br enhances the dispersion of silica in natural rubber. [EPy]Br significantly reduces the duration of the induction period for vulcanization. The dosage of [EPy]Br allows for controlling the mechanical properties of NR/SiO 2 .
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JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2024
·
IF 5.8
Haihan Huang,Jie Zheng,Kaiyue Wu,Jian Lin,Xin Lin,Quan Yao,Qianyu Fan,Dechao Wang,Yuanbo Huang,Jianchun Jiang,Zhifeng Zheng
Catalytic pyrolysis technology is one of the most environmental and efficient approach in converting biomass waste into high-value chemicals and fuels. In this study, the ex-situ catalytic pyrolysis of cellulose to produce furans and olefins was reported using various metal-loaded TS-1 as catalysts. The experimental results showed that the Au/TS-1 exhibited an exceptional catalytic activity in the production of furans and olefins, achieving a selectivity of 49.17 % and 40.65 %, respectively. Ni/TS-1 effectively promoted the formation of ethylene, yielding a selectivity of 97.49 % and a yield of 6.11 C-mol%. Under the optimized conditions: pyrolysis temperature of 550 ℃, catalytic temperature of 600 ℃, and catalyst to cellulose mass ratio of 20:1, the Au/TS-1 catalyst achieved a furans selectivity of 41.91 % with a yield of 11.94 C-mol%, while olefins selectivity and yield were 49.84 % and 18.99 C-mol%, respectively. At this condition, Au/TS-1 still has excellent catalytic activity after 10 cycles and has good catalytic effect towards different biomass feedstocks. Furthermore, the possible mechanism to produce furans and olefins was revealed. This research offers valuable insights for guiding the directed conversion of biomass into value-added chemicals, paving the way for a more sustainable future.
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Regenerative Therapy
·
2024
·
IF 3.4
Min Ge,Li Sun,Defeng Wang,Chunchao Hei,Tingjuan Huang,Zhongxin Xu,Qizhi Shuai
The use of stem cell-based treatment systems is prevalent in regenerative medicine. To enhance the regenerative capabilities of stem cells, growth factors are typically incorporated into the treatment system. Nonetheless, traditional hydrogel-encapsulated or heparinized scaffolds that bind factors have limitations. In this study, we prepared a biomaterial strategy using uniform poly(lactic-co-glycolic) acid (PLGA) microspheres (uPLGA-Ms) fabricated by microfluidic to sustain delivery of insulin-like growth factor 1 (IGF-1), a critical protein for hMSCs biological functions. The uPLGA-Ms loaded IGF-1 were highly monodispersed through precise manipulation of the flow rate of the two-phase of the flow-focusing microchannle. The results showed that the uPLGA-Ms stabilize IGF-1 and provide a more efficient sustained delivery and cost-effective of growth factor. Gene expression analysis demonstrated the uPLGA delivery of IGF-1 results in a (enhanced) supported hMSCs expansion, survival, stemness, and secretion abilities comparable with the conventional soluble IGF-1 group. In summary, this material-based strategy to stabilize and sustain delivery of growth factor has broad potential to regeneration of various tissues and organs.
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高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Huakang Yang,Zining Zhang,Yudong Guo,Shuwen Yuan,Xiao Liu,Dongxiang Luo,Siyu Ye
Solar-driven photocatalytic CO 2 reduction coupled with the oxidation of organic compounds to value-added chemicals is a promising strategy, which can utilize both photogenerated electrons and holes. Here, a gradient alloyed CdS@Zn x Cd 1-x S@ZnS quantum dots (quasi-Type II band structure) capped with Cl − ligands demonstrate great potential in the simultaneous conversion of CO 2 and 1-phenylethanol into syngas and pinacol. The passivation with Cl − maximizes the exposure of surface-active sites and improves their electronic structure, providing a prerequisite for the efficient execution of redox reactions. More importantly, the p–π conjugation between chloride-ion ligands and the benzene ring provides a bridge for the ultrafast transfer of holes, which greatly promotes the activation of the Cα─H bond in 1-phenylethanol to release a mass of reactive free hydrogen. This process effectively lowers the thermodynamic energy barrier for CO 2 reduction, even as the excessively high kinetic energy barriers arising from the accumulation of excess protons also enable them to self-couple, leading to the generation of syngas (CO ≈ 75 mmol g −1 h −1 ; selectivity ≈ 69.86%). This work provides insights for future artificial photosynthesis to achieve high activity and cost-effective conversion of CO 2 into storable renewable resources and the co-production of value-added substances through synergistic organic oxidation.
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NANOTECHNOLOGY
·
2024
·Vol. 35
·期 41
·415501
·
IF 2.9
Yanxin Liao,Shupei Sun,Qian Du,Nianfeng Shi,Guangfu Yin,Zhongbing Huang,Xiaoming Liao
Metal–organic frameworks (MOFs) are believed to be promising precursors for constructing novel and efficient catalysts for glucose sensing. Herein, HKUST-1 precursors are first fabricated using a one-pot hydrothermal approach, and then HKUST-1 is converted into porous Cu2S/CuO octahedrons through conformal sulfidation with the help of OH− ions. The as-obtained Cu2S/CuO composite can provide rich electrochemical active sites and promoted electric transfer kinetics. Benefiting from these combined merits, the as-fabricated Cu2S/CuO composite is confirmed to be a high-performance catalyst, with high sensitivities of 8269.45 and 4140.82 μA mM−1cm−2 in the corresponding ranges of 0.05 ∼ 0.6 mM and 0.6 ∼ 1.2 mM, respectively. Moreover, the as-prepared electrode materials possess good anti-interference ability, reproducibility and long-term stability. This work opens up new avenues for the design and preparation of transition metal sulfide composites.
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高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2024
·
IF 2
Yong Zheng,Jiahuan Luo,Ling Li,Qian Wang,Huichao Lv,Kai Wang,Changwei Lai
In this study, six pure 1-alkyl-2-methylpyrazolium-based ionic liquids (ILs) with methyl sulfate and bis(trifluoromethylsulfonyl)imide anions ([Cnmpz][MeSO4] and [Cnmpz][NTf2], n = 2, 3, and 4) were prepared and characterized. The density, viscosity, and refractive index of these pure ILs were measured from 298.15 to 353.15 K at 101.3 kPa, which fitted well with the corresponding empirical equations. Moreover, the values of the isobaric thermal expansion coefficient were also obtained. The results of experiments and density functional theory calculations indicate that the adjacent positions of two nitrogen atoms in pyrazolium cations lead to the close packing of ions, strong cation–anion interaction, and high molar polarizability per unit volume. The effects of temperature and N-alkyl chain length on the density, viscosity, and refractive index of these ILs are similar to those for imidazolium-based salts. Compared with [Cnmpz][MeSO4], [Cnmpz][NTf2] exhibits a higher density, lower viscosity, and lower refractive index under the same conditions. Moreover, the correlation between density and refractive index was studied, and a new empirical equation was proposed to express this correlation at different temperatures. It is expected that this work will enable deeper insights into the properties, structure, and structure–property relationship of pure pyrazolium-based ILs.
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INORGANIC CHEMISTRY
·
2025
·
IF 4.7
Yong Song,Wenjia Hao,Jinsong Li,Bo Jin,Rufang Peng
Hierarchical porous metal–organic frameworks (HP-MOFs) have attracted considerable attention because of their hierarchical pores, which can address the slow mass transfer and less exposure of active sites in pristine microporous MOFs. Although several preparation methods have been developed to date, a large-scale technique for the synthesis of HP-MOFs is still lacking. In this study, we report a novel method for the large-scale synthesis of HP-HKUST-1 based on liquid-assisted spiral gas–solid two-phase flow (LA-S-GSF). This method ingeniously uses a nebulizer to promote the rapid synthesis of nano-HKUST-1 by introducing trace amounts of water during the S-GSF reaction. During the washing and drying process, these nanoparticles were fully fused to form nanocrystalline aggregates, resulting in a hierarchical porous structure with a large number of micropores, mesopores, and macropores. The pore size distribution can be regulated by changing the drying temperature to obtain HP products with combinations of micropores and mesopores, micropores and macropores, and the formation mechanism of the HP structure was also explored. This method required only 11 min of reaction time to obtain 25.2 g of HP-HKUST-1 in 96% yield, with a corresponding space-time yield (STY) of 6.9 × 104 kg m–3 day–1.
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高影响因子
Cell Death & Disease
·
2017
·Vol. 8
·期 8
·e2988
·
IF 5.965
Yan Siyuan,Liu Ling,Ren Fengxia,Gao Quan,Xu Shanshan,Hou Bolin,Wang Yange,Jiang Xuejun,Che Yongsheng
Deficiency of autophagy has been linked to increase in nuclear instability, but the role of autophagy in regulating the formation and elimination of micronuclei, a diagnostic marker for genomic instability, is limited in mammalian cells. Utilizing immunostaining and subcellular fractionation, we found that either LC3-II or the phosphorylated Ulk1 localized in nuclei, and immunoprecipitation results showed that both LC3 and Unc-51-like kinase 1 (Ulk1) interacted with γ -H2AX, a marker for the DNA double-strand breaks (DSB). Sunitinib, a multi-targeted receptor tyrosine kinase inhibitor, was found to enhance the autophagic flux concurring with increase in the frequency of micronuclei accrued upon inhibition of autophagy, and similar results were also obtained in the rasfonin-treated cells. Moreover, the punctate LC3 staining colocalized with micronuclei. Unexpectedly, deprivation of SQSTM1/p62 alone accumulated micronuclei, which was not further increased upon challenge with ST. Rad51 is a protein central to repairing DSB by homologous recombination and treatment with ST or rasfonin decreased its expression. In several cell lines, p62 appeared in the immunoprecipites of Rad51, whereas LC3, Ulk1 and p62 interacted with PARP-1, another protein involved in DNA repair and genomic stability. In addition, knockdown of either Rad51 or PARP-1 completely inhibited the ST-induced autophagic flux. Taken together, the data presented here demonstrated that both LC3-II and the phosphorylated Ulk1 localized in nuclei and interacted with the proteins essential for nuclear stability, thereby revealing a more intimate relationship between autophagy and genomic stability.
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高影响因子
Macromolecular Reaction Engineering
·
2017
·Vol. 11
·期 5
·1600055
·
IF 1.571
Qiaoqiao Sun,Lisong Wang,Ruihua Cheng,Zhen Liu,Xuelian He,Ning Zhao,Boping Liu
Phillips catalyst is one of the most significant industrial ethylene polymerization catalysts. Chemical modifications have been carried out to tune the Phillips catalyst performance and improve the polyethylene properties. After the modification of the catalyst by fluorine, the polyethylene product with higher molecular weight (MW) and narrower molecular weight distribution (MWD) is suitable for producing automobile fuel tanks. Vanadium containing Phillips catalyst enhances α-olefin incorporation and MW regulation. In present work, fluorine modified and unmodified chromium–vanadium (Cr–V) bimetallic catalysts are prepared and explored. Compared with the fluorine-free catalyst, the activities of F-modified bimetallic catalysts slightly decrease with the increasing MW of the product and the hydrogen response increases slightly. Due to the synergistic effect of the chromium, vanadium and fluorine on the silica gel support, the short-chain branch distribution (SCBD) of copolymers from F-modified Cr–V bimetallic catalyst (Cr–V–F) 600 is more beneficial than that of Cr–V bimetallic catalyst (Cr–V) 600 and F-modified Cr–V bimetallic catalyst (Cr–V–F) 500 . The fluorination of Cr–V bimetallic catalysts has not only preserved the high polyethylene activity of bimetallic active sites but also produced the advantage of the high MW ability from fluorine.
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高影响因子
Molecular Catalysis
·
2025
·
IF 4.9
Jingyi Chen,Wenyu Shao,Ziyu Zhang,Zhen Gao,Jiaxing Xu,Bin Wu
Processive endoglucanase with both endoglucanase and exoglucanase activity exhibits great potential in the degradation of cellulose. Carbohydrate Binding Modules (CBMs) can improve the catalytic efficiency by positioning the substrate closer to the catalytic domain and boosting the affinity between the enzyme and substrate. To investigate the impact of CBMs and linker peptides on the catalytic performance of the processive endoglucanase EG5C-1, various EG5C-1 derivatives with different CBMs and linker peptides were constructed. The results suggested the introduction of CBM1 from Volvariella volvacea and flexible peptide (GGGGS) 3 led to a notable 3.4-fold increase on the Avicel and a 3.7-fold increase on the overall enzymatic activity (FPase). In addition, the kinetic parameters of EG5C-1-F3- Vv CBM1 showed higher affinity ( K m ) and higher catalytic efficiency ( k cat / K m ) for insoluble substrates. Moreover, inverse kinetic parameters indicated that 99.5% of attack sites were occupied, surpassing the wild type. The processivity enhanced by 60% compared to EG5C-1. A series of cellooligosaccharides were hydrolyzed, with a pattern similar to EG5C-1. These results suggest that the fusing CBMs and linker peptides to the catalytic domain could effectively design a highly efficient processive endoglucanase.
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