ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2024
·
IF 6.2
Huazhong Zhang,Hao Sun,Jian Qian,Li Sun,Cheng Zong,Jinsong Zhang,Beilei Yuan
Diquat (DQ) is a commonly used bipyridine herbicide known for its toxic properties and adverse effects on individuals. However, the mechanism underlying DQ-induced damage remain elusive. Our research aimed to uncover the regulatory network involved in DQ-induced damage. We analyzed publicly accessible gene expression patterns and performed research using a DQ-induced damage animal model. The GSE153959 dataset from the Gene Expression Omnibus collection and the animal model of DQ-induced kidney injury were used to identify differentially expressed genes (DEGs). Pathways including the regulation of DNA-templated transcription in response to stress, RNA polymerase II transcription regulator complex and transcription coregulatory activity were shown to be enriched in 21 DEGs. We used least absolute shrinkage and selection operator (LASSO) regression analysis to find possible diagnostic biomarkers for DQ-induced damage. Then, we used an HK-2 cell model to confirm these results. Additionally, we confirmed that 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2) was the major gene associated with DQ-induced damage using multi-omics screening. The sample validation strongly suggested that HMGCS2 has promise as a diagnostic marker and may provide new targets for therapy in the context of DQ-induced damage.
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高影响因子
Nanomaterials
·
2024
·Vol. 14
·期 15
·1240
·
IF 4.4
Dongmei Zhang,Jiaqi Zhao,Yangyang Peng,Yuchao Li,Wenbin Guo,Chengzhu Liao
Water pollution seriously affects the development of society and human life. There are various kinds of pollutants, including soluble pollutants and insoluble floaters on the water surface. Herein, the photocatalyst semiconductor BiOCl and superhydrophobic functional particles Mg(OH)2were deposited on the surfaces of canvas and polyester felt to construct superhydrophobic canvas and polyester felt. The contact angles of the synthetic superhydrophobic canvas and polyester felt were measured as 152° and 155.3°, respectively. The selective adsorption of hexadecane was achieved using the wetting difference between the surface of water and pollutants floating on the surface. For dissolved pollutants, the surface wettability needed to be changed with the help of ethanol. The degradation efficiencies were all greater than 90%, demonstrating the versatility of the synthetic superhydrophobic canvas and polyester felt.
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JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
·
2025
·
IF 4.3
Fan Yanan,An Caizhelin,Wang Zhihui,Luo Jia,Wang Wenbin,Luo Qing,Song Guanbin
Hepatocytes are the primary functional cells in the liver, and the malignant transformation of hepatocytes significantly contributes to hepatocellular carcinoma (HCC) progression. Liver fibrosis and cirrhosis caused by extracellular matrix (ECM) remodeling during liver lesions is a pivotal driver of HCC. However, the impact of matrix stiffness on hepatocytes and the underlying molecular mechanisms are not fully understood. Herein, using gelatin/sodium alginate hydrogels with different stiffnesses to simulate the change of matrix stiffness during liver lesions, we found that matrix stiffening leads to a notable decrease in the expression of hepatocyte nuclear factor 4α (HNF4α) and functional hepatocyte genes and a significant increase in the expression of interleukin 6 (IL‒6) in human hepatocyte line L‒02 cells, indicating obvious damage of hepatocyte function. In addition, matrix stiffening causes extensive DNA damage to L‒02 cells. Mechanistically, matrix stiffening upregulates piezo‒type mechanosensitive ion channel component 1 (Piezo1) expression and activates extracellular signal‒regulated kinase 1/2 (ERK1/2) signaling. Piezo1 knockdown suppresses matrix stiffening‒induced functional impairment and DNA damage in L‒02 cells. Moreover, Piezo1 knockdown blocks matrix stiffening‒activated ERK1/2 signaling in L‒02 cells. U0126 (a selective inhibitor of ERK1/2 activation) treatment could rescue matrix stiffening‒induced functional impairment and DNA damage. Taken together, these findings demonstrate that matrix stiffening induces functional impairment and DNA damage in L‒02 cells via the Piezo1‒ERK1/2 signaling pathway, which provides evidence for a better understanding of the hepatocyte function damage caused by tissue mechanical microenvironment change in liver diseases and the mechanotransduction in this process. Graphical abstract An increase in matrix stiffness inhibits HNF4α expression and promotes IL‒6 expression in L‒02 cells. Meanwhile, matrix stiffening induces L‒02 cell DNA damage and upregulates the expression of γ‒H2AX and 53BP1. These findings prove that matrix stiffness is a crucial mechanical regulator of cellular functional impairment and DNA damage and plays an important role in liver disease.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Yanan Zhang,Fei Meng,Tiantian Zhao,Jingyi Du,Naigang Li,Xinghui Qiao,Yuan Yao,Dong Wu,Fan Peng,Dongshuang Wang,Shuang Yang,Jiaming Shi,Ruoxi Liu,Wenjuan Zhou,Lei Li,Aijun Hao
2-Ethylhexyl diphenyl phosphate (EHDPP) is a representative organophosphorus flame retardant (OPFR) that has garnered attention due to its widespread use and potential adverse effects. EHDPP exhibits cytotoxicity, genotoxicity, developmental toxicity, and endocrine disruption. However, the toxicity of EHDPP in mammalian oocytes and the underlying mechanisms remain poorly understood. Melatonin is a natural free radical scavenger that has demonstrated cytoprotective properties. In this study, we investigated the effect of EHDPP on mouse oocytes in vitro culture system and evaluated the rescue effect of melatonin on oocytes exposed to EHDPP. Our results indicated that EHDPP disrupted oocyte maturation, resulting in the majority of oocytes arrested at the metaphase I (MI) stage, accompanied by cytoskeletal damage and elevated levels of reactive oxygen species (ROS). Nevertheless, melatonin supplementation partially rescued EHDPP-induced mouse oocyte maturation impairment. Results of single-cell RNA sequencing (scRNA-seq) analysis elucidated potential mechanisms underlying these protective effects. According to the results of scRNA-seq, we conducted further tests and found that EHDPP primarily disrupts mitochondrial distribution and function, kinetochore-microtubule (K-MT) attachment, DNA damage, apoptosis, and histone modification, which were rescued upon the supplementation of melatonin. This study reveals the mechanisms of EHDPP on female reproduction and indicates the efficacy of melatonin as a therapeutic intervention for EHDPP-induced defects in mouse oocytes.
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Materials Today Communications
·
2025
·
IF 4.5
Yi Zhang,Hao Wang,Man Li,Xinhao Sun,Yan Zhang,Zhifeng Wang,Chunling Qin
This work successfully synthesizes a new type of multi-layered flexible hybrid electrode (Cu/Ni(OH) 2 /np-Ni) composing of bimetallic components, hierarchical porous structure and outstanding glucose electrocatalytic performance. The synthesis strategy includes a facile two-step approach, i.e. the Ni(OH) 2 nanolayer in-situ grows on a nanoporous Ni (np-Ni) network (Ni(OH) 2 /np-Ni) via deionized water immersion and then the metallic Cu dendrites are uniformly deposited on Ni(OH) 2 /np-Ni. Moreover, due to the hierarchical porous structure as well as synergetic effect of Ni(OH) 2 and Cu dual electroactive components, the Cu/Ni(OH) 2 /np-Ni hybrid electrode electrodeposited with 1 min delivers wider linear range of 18 mM and higher sensitivity of 1.629 mA mM −1 cm −2 . The electrode possesses excellent interference immunity, reproducibility as well as long-time stability. In addition, the mechanism of electrochemical sensors for detecting glucose under alkaline conditions is briefly introduced. Accordingly, the flexible Cu/Ni(OH) 2 /np-Ni electrode material shows promise for further development as a contender for wearable glucose sensors.
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Small
·
2024
·
IF 13
Huacheng He,Wen Huang,Shihui Zhang,Jie Li,Jian Zhang,Bingxin Li,Jie Xu,Yuting Luo,Huiling Shi,Yue Li,Jian Xiao,Odinaka Cassandra Ezekiel,Xiaokun Li,Jiang Wu
Severe burn wounds usually destroy key cells’ functions of the skin resulting in delayed re-epithelization and wound regeneration. Promoting key cells’ activities is crucial for burn wound repair. It is well known that keratinocyte growth factor-2 (KGF-2) participates in the proliferation and morphogenesis of epithelial cells while acidic fibroblast growth factor (aFGF) is a key mediator for fibroblast and endothelial cell growth and differentiation. However, thick eschar and the harsh environment of a burn wound often decrease the delivery efficiency of fibroblast growth factor (FGF) to the wound site. Therefore, herein a novel microneedle patch for sequential transdermal delivery of KGF-2 and aFGF is fabricated to enhance burn wound therapy. aFGF is first loaded in the nanoparticle (NP aFGF ) and then encapsulated NP aFGF with KGF-2 in the microneedle patch (KGF-2/NP aFGF @MN). The result shows that KGF-2/NP aFGF @MN can successfully get across the eschar and sequentially release KGF-2 and aFGF. Additional data demonstrated that KGF-2/NP aFGF @MN achieved a quicker wound closure rate with reduced necrotic tissues, faster re-epithelialization, enhanced collagen deposition, and increased neo-vascularization. Further evidence suggests that improved wound healing is regulated by significantly elevated expressions of hypoxia-inducible factor-1 alpha (HIF-1ɑ) and heat shock protein 90 (Hsp90) in burn wounds. All these data proved that KGF-2/NP aFGF @MN is an effective treatment for wound healing of burns.
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Drug Design Development and Therapy
·
2024
·
IF 4.7
Yong-Yu Yang,Rong-Rong Deng,Da-Xiong Xiang
Background Ferroptosis plays a crucial role in the occurrence and development of cerebral ischemia-reperfusion (I/R) injury and is regulated by mitogen-activated protein kinase 1/2 (ERK1/2). In China, Naodesheng Pills (NDSP) are prescribed to prevent and treat cerebrosclerosis and stroke. However, the protective effects and mechanism of action of NDSP against cerebral I/R-induced ferroptosis remain unclear. We investigated whether NDSP exerts its protective effects against I/R injury by regulating ferroptosis and aimed to elucidate the underlying mechanisms.Methods The efficacy of NDSP was evaluated using a Sprague-Dawley rat model of middle cerebral artery occlusion and an in vitro oxygen-glucose deprivation/reoxygenation (OGD/R) model. Brain injury was assessed using 2,3,5-triphenyltetrazolium chloride (TTC), hematoxylin and eosin staining, Nissl staining, and neurological scoring. Western blotting was performed to determine the expression levels of glutathione peroxidase 4 (GPX4), divalent metal-ion transporter-1 (DMT1), solute carrier family 7 member 11 (SLC7A11), and transferrin receptor 1 (TFR1). Iron levels, oxidative stress, and mitochondrial morphology were also evaluated. Network pharmacology was used to assess the associated mechanisms.Results NDSP (1.08 g/kg) significantly improved cerebral infarct area, cerebral water content, neurological scores, and cerebral tissue damage. Furthermore, NDSP inhibited I/R- and OGD/R-induced ferroptosis, as evidenced by the increased protein expression of GPX4 and SLC7A11, suppression of TFR1 and DMT1, and an overall reduction in oxidative stress and Fe2+ levels. The protective effects of NDSP in vitro were abolished by the GPX4 inhibitor RSL3. Network pharmacology analysis revealed that ERK1/2 was the core target gene and that NDSP reduced the amount of phosphorylated ERK1/2.Conclusion NDSP exerts its protective effects against I/R by inhibiting cerebral I/R-induced ferroptosis, and this mechanism is associated with the regulation of ferroptosis via the ERK1/2 signaling pathway.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yi Liu,Shaoyan Wang,Jiarui Liang,Lirong Lu,Yi Xie,Chengrong Qin,Chen Liang,Caoxing Huang,Shuangquan Yao
Demethylation modification of lignin is an effective strategy to overcome the barrier to its high-value conversion. The purpose of this study focuses on the new proton-based ionic liquid (PIL) 1, 2-propanediamine/glycolic acid (PD/GA) as a catalyst and solvent to achieve the targeted oxidation of lignin. The PD/GA solvents have higher selectivity and efficiency. Optimal phenolic hydroxyl (PH)-increment was achieved, demonstrating enhanced demethylating effect on lignin by modulating the acid-base molar ratio, reaction temperature, and reaction time. Compared to ethanolamine/acetic acid (CE/AC) treatment, the PD/GA treatment at molar ratio 1.25, temperature 60 °C, and 3 h increased the PH-content from 37.74 to 59.91 %. Additionally, the lignin treated with PD/GA exhibited excellent recyclability, featuring a larger Brunauer-Emmett-Teller surface area (1.45 m 2 .g −1 ), total pore volume (9.51*10 −3 cm 3 .g −1 ), and mesoporous size (26.15 nm). The treated lignin yielded maximum ultraviolet resistance and antioxidant activity. These results present new avenues for the development of green and efficient lignin demethylation methods.
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JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2024
·Vol. 171
·期 9
·096505
·
IF 3.1
Zhefei Zhao,Linlin Zhang,Minghao Chen,Ruopeng Yu,Xuyao Yao,Yinghua Xu,Youqun Chu,Xinbiao Mao,Huajun Zheng
Electrochemical selective dechlorination can be regarded as one of the most promising strategies for generating high-valued chemicals. In the electrochemical dechlorination process of 2-chloro-5-trichloromethylpyridine (TCMP), except the anticipated dechlorination products involving 2-chloro-5-dichloromethylpyridine (DCMP), 2-chloro-5-chloromethylpyridine (CCMP), and 2-chloro-5-methylpyridine (CMP), some unexpected oxygen-incorporated products (6-chloronicotinic acid (CNA) and 6-chloronicotinoyl methyl ester (MCN)) can be obtained. Consequently, understanding the electrochemical dechlorination behavior of TCMP is crucial. Our research revealed that the activated Ag electrodes in halide ion solution exhibit enhanced electrochemical activities for electrochemical dechlorination of TCMP, compared with the pure Ag owing to the increased active specific surface areas and charge transfer. Second, oxygen participation in the reaction is a necessary condition for the formation of oxygen-incorporated products. A 100% selectivity of oxygen-incorporated products can be obtained at the potential of −0.6 V vs Ag/AgCl. Conversely, insufficient oxygen may lead to the potential becoming the determining condition that affects the reaction pathways. A more negative potential (−1.2 V vs Ag/AgCl) is conducive to the formation of dechlorination products with 94.2% conversion and 100% selectivity. This study, for the first time, elucidates the electrocatalyst, atmosphere, and potential-dependent activity and selectivity for the two dechlorination pathways of TCMP.
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CHINESE JOURNAL OF POLYMER SCIENCE
·
2018
·Vol. 36
·期 5
·612-619
·
IF 2.016
Wang Ye,Hu Ting-Ting,Han Xiao-Long,Wang Yu-Qi,Li Ji-Ding
Cu(OH) 2 nanowires were prepared and incorporated into poly(vinylidene fluoride) (PVDF) to fabricate Cu(OH) 2 -PVDF ultrafiltration (UF) membrane via immersion precipitation phase inversion process. The effect of Cu(OH) 2 nanowires on the morphology of membranes was investigated by X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM), scanning electron microscopy (SEM) and X-ray diffraction (XRD) measurements. The results showed that all the Cu(OH) 2 -PVDF membranes had wider fingerlike pore structure and better hydrophilicity, smoother surface than pristine PVDF membrane due to the incorporation of Cu(OH) 2 nanowires. In addition, water flux and bovine serum albumin (BSA) rejection were also measured to investigate the filtration performance of membranes. The results indicated that all the Cu(OH) 2 -PVDF membranes had high water flux, outstanding BSA rejection and excellent antifouling properties. It is worth mentioning that the optimized performance could be obtained when the Cu(OH) 2 nanowires content reached 1.2 wt%. Furthermore, the membrane with 1.2 wt% Cu(OH) 2 nanowires showed outstanding oil-water emulsion separation capability.
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Polymers
·
2018
·Vol. 10
·期 2
·159
·
IF 2.935
Chao Xiong,Shixing Wang,Libo Zhang,Ying Li,Yang Zhou,Jinhui Peng
A new adsorbent(A-PGMA) has been synthesized via functionalizing poly(glycidyl methacrylate) microsphere with 2-aminothiazole and used to adsorb gold ions from aqueous solutions. The adsorbent was characterized by X-ray photoelectron spectroscopy (XPS), Brunauer–Emmett–Teller (BET), Zeta potential, scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FT-IR). The influence factors such as the pH value of the solution, the initial gold ion concentration and the contact time were examined. Simultaneously, the adsorption process of the gold ion on A-PGMA fitted well with the Langmuir and pseudo-second-order models, respectively. The results showed that the maximum adsorption capacity was 440.54 mg/g and the equilibrium time of adsorption was about 3 h under pH 4. Moreover, the adsorbent has a high reusability after five cycles and good selectivity from coexisting ions, including Zn(II), Mg(II), Cu(II), Ge(IV) and B(III). The adsorption mechanisms of gold ions were ion exchange and chelation between the sulfur and nitrogen groups on the surface of A-PGMA and AuCl4−. Therefore, the adsorbent has a great potential for adsorption of gold ions from aqueous solutions.Keywords:adsorption;gold;reusability;mechanism;selectivity
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Jiaxin Huang,Chongshuai Gao,Shijun Liu,Xiaorui Du,Wenguang Zhou,Chenguang Wang
Alloying Cu-based catalysts by doping other metals at single atom level is an effective way to improve their hydrogenation performance of biomass-derived molecules. However, the application of Cu-based single atom alloy catalyst in the catalytic hydrogen transfer reaction system remains to be unrevealed. Here, we develop Al 2 O 3 supported CuPd alloy catalysts, among which the single atom alloy catalyst with Pd dispersed in single atomic level on the surface was successfully obtained by controlling the metal amount, such as Cu 8 Pd 1 /Al 2 O 3 with the Cu/Pd Molar ratio of 8: 1. The catalytic performance for furfural hydrodeoxygenation to 2-methylfuran of the CuPd/Al 2 O 3 catalysts, using isopropanol as a hydrogen donor, shows a volcano-type relationship to the Cu/Pd ratio. The highest 2-methylfuran yield (81% at 220 °C) was achieved on the single atom alloy catalyst Cu 8 Pd 1 /Al 2 O 3 . The underlying reaction mechanism of furfural hydrodeoxidation on Cu 8 Pd 1 /Al 2 O 3 was revealed by a series of isotopic labeling experiments, that the furfural was firstly hydrogenated to be furfuralcohol by a metal site-mediated catalytic transfer hydrogenation pathway, and then the furfuralcohol was converted to be 2-methylfuran by a Lewis acid-mediated furan ring activation route. The weak Lewis acid site that formed by single atom Pd alloyed with CuO matrix on Cu 8 Pd 1 /Al 2 O 3 was proposed as the active site for the highly selective generation of 2-methylfuran.
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危险品化学品经营许可证（带存储）