Environmental Technology & Innovation
·
2026
·
IF 7.3
Wenjie Wang,Xulong Fan,Yaquan Cao,Ruolin Shi,Ping Wang,Gaofeng Liang
Benzophenone-3 (BP-3), a common ultraviolet (UV) filter widely used in sunscreens, has raised increasing concern because early-life exposure may interfere with normal neurodevelopment. In this study, we fabricated a 3D inverse-opal polyethylene glycol diacrylate (PEGDA) scaffold and established a scaffold-supported brain organoid model for in vitro evaluation of BP-3-induced neurotoxicity. The scaffold was characterized by scanning electron microscopy (SEM), which revealed a highly ordered porous morphology with uniform pore size. It also showed good biocompatibility for organoid culture. BP-3 exposure induced concentration- and time-dependent toxicity in brain organoids, with more pronounced effects observed at 50 and 100 μM after 72 h of exposure. Under these defined exposure conditions, BP-3 was associated with increased oxidative stress, calcium-related functional changes, impaired membrane integrity, increased cytotoxicity, reduced expression of synaptic structural markers, and altered Wnt/β-catenin-related protein expression. These findings suggest that BP-3 exposure may disrupt early neurodevelopment-related processes in this model and is associated with synaptic-related alterations and Wnt/β-catenin dysregulation. Overall, this study supports the applicability of scaffold-supported brain organoids as an in vitro platform for evaluating BP-3-induced neurotoxic responses under controlled experimental conditions.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Bin Ma,Xuehui Guo,Huibing Chi,Jiafeng Niu,Xiaoyu Zhu,Juan Shen,Hao Zhu,Ping Zhu,Fengxia Lu,Yanzi Zhou
Aldo-keto reductase AKR13B3 catalyzes the reduction of 3-keto-deoxynivalenol (3-keto-DON) to nontoxic 3-epi-DON, thereby alleviating mycotoxin contamination in food and feed. However, the previously reported AKR13B3 mutant AKR-V3 exhibited high catalytic activity but poor thermostability, restricting its industrial application. Here, guided by molecular dynamics flexibility analysis and consensus sequence design, we engineered the variant M5 with markedly improved thermostability. M5 showed a 38.3-fold longer half-life at 60 °C and retained slightly enhanced catalytic efficiency. Quantum mechanics/molecular mechanics (QM/MM) calculations revealed that M5 possessed a lower activation barrier than AKR-V3, which explained its superior activity retention under high-temperature conditions. Moreover, structural analyses and molecular dynamics (MD) simulations further demonstrated that the enhanced thermostability of M5 stemmed from reinforced hydrogen bonding, increased hydrophobic packing, elevated structural rigidity, and optimized surface electrostatic potential. This work provides an effective strategy for designing thermostable detoxification enzymes to eliminate mycotoxins in industrial applications.
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高影响因子
ELECTROCHIMICA ACTA
·
2026
·
IF 5.8
Zilong Fang,Qi Fan,Anyang Yu,Wei Zhou,Junwei Luo,Changhao Zhu,Xinguantong Zhou,Xiao Yu,Qingyu Xu
Achieving uniform ion flux both at the interface and in the matrix simultaneously remains a challenge with current liquid/solid electrolytes. Herein, we demonstrate a novel strategy to address the issue by designing and synthesizing a polyvinylidene fluoride hexafluoropropylene (PVDF-HFP) based ternary hybrid electrolyte (PTHE). In this unique material architecture, each component synergistically serves a specific purpose: The PVDF-HFP-Zn(OTF) 2 serves as the stable electrolyte skeleton, providing the ion transport network. The Cu-MOF regulates the Zn 2+ solvation structure and anion distribution, achieving uniform ion flux in the electrolyte matrix. Moreover, the thin liquid electrolyte film at the interface promotes ion transport and stabilizes the electrode-electrolyte interface. The PTHE exhibits balanced high performance with respect to cycling stability (3200 h at 0.2 mA cm −2 ) and excellent capacity retention when paired with the Na 3 V 2 (PO 4 ) 3 (NVP) cathode (0.0029 % capacity decay per cycle after 2000 cycles at 0.5 A g −1 ), alongside uniform Zn metal deposition and effective dendrite suppression. Furthermore, the PTHE based NVP-Zn metal quasi-solid battery demonstrates outstanding flexibility (enduring 1000 bending cycles with less than 10% decrease in capacity retention), owing to the existence of the thin liquid electrolyte film. This work provides a novel strategy for developing highly stable flexible NVP-Zn metal quasi-solid batteries by synergistically optimizing ion flux both at the interface and in the matrix.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Yi Duan,Meilin Liu,Dianhui Men,Yiyao Luo,Xuhui Xia,Li Wang,Fei Deng,Danqun Huo,Changjun Hou
CA15–3 is a vital biomarker for monitoring and evaluating treatment efficacy in breast cancer patients, especially those with advanced-stage disease. Conventional immunoassays face limitations, including high cost, limited sensitivity, and dependence on potentially error-prone single-fluorescence signal outputs. To address these challenges, we designed a dual-signal-emitting sensor based on CdTe quantum dots@ZIF-8 nanomaterials, incorporating both the CRISPR/Cas12a system and catalytic hairpin assembly (CHA) technology. The binding of CA15–3 to its specific aptamer inhibits the CHA cascade reaction, thereby preventing the generation of double-stranded DNA activators necessary to initiate Cas12a-mediated trans-cleavage activity. As a result, FAM-ssDNA is extensively adsorbed onto the CdTe QDs@ZIF-8 surface, where efficient electron transfer effectively quenches its fluorescence. The incorporation of the aptamer facilitates dual-signal amplification through both the CHA and CRISPR/Cas12a systems, achieving a detection limit of 0.131 U/mL. This dual-signal output mechanism provides the system with an internal reference function, thereby significantly improving the reliability of the results. Overall, this work establishes a novel tumor biomarker detection platform that combines high sensitivity with excellent robustness.
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JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
·
2026
·
IF 3.4
Bo Zhou,Xiushang Ye,Yuan Tian,Xintong Wang,Xiaozhang Xu,Yi Ren
Acetaminophen (APAP)-induced liver injury is a major cause of acute liver injury (ALI). Indole-3-propionic acid (IPA), a gut microbiota–derived tryptophan metabolite with potent anti-inflammatory and antioxidant properties, has not been fully explored for its therapeutic potential in APAP-induced ALI. APAP-induced liver injury is often accompanied by dysregulation of both the gut microbiota and the intrahepatic immune system. In our study, IPA levels were markedly reduced in the ALI model, reflecting impaired gut tryptophan metabolism. In vitro and in vivo experiments demonstrated that IPA mitigates cytotoxicity, reduces hepatocyte apoptosis, and alleviates liver inflammation, thereby conferring significant hepatoprotective effects. The aryl hydrocarbon receptor (AhR), a key mediator of IPA’s effects, was substantially downregulated in APAP-induced ALI and negatively correlated with serum AST and ALT levels, suggesting that AhR may serve as a potential early biomarker for APAP-induced hepatotoxicity. Notably, IPA treatment restored hepatic and serum AhR expression and attenuated liver injury. Collectively, these findings underscore the critical role of IPA in modulating AhR signaling and highlight its therapeutic potential in APAP-induced ALI.
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PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2026
·
IF 6.6
Xianyue Zhang,Yongguang Zhang,Nianxue Dong,Jiawei Fan,Haipeng Li,Baoe Li
The slow Na + diffusion and low intrinsic electrical conductivity of Na 3 V 2 (PO 4 ) 3 (NVP) limit its practical application in sodium-ion batteries (SIBs). A synergistic enhancement approach of Si 4+ substitution and N-doped carbon coating has been introduced in this paper to produce a porous coral-like Na 3.1 V 2 (PO 4 ) 2.9 (SiO 4 ) 0.1 @NC-2 (NVPS@NC-2) composite. Si 4+ incorporation into the NVP lattice expands the Na + migration channels and decreases the bandgap so that intrinsic electronic conductivity of NVP was enhanced as confirmed by density functional theory calculations. Additionally, the N-doped carbon coating forms numerous extrinsic defects and creates a novel porous coral-like morphology that boosts the specific surface area. The initial specific capacity of NVPS@NC-2 cathode achieves 115.7 mAh g −1 at a current density of 0.1C. And even at 60/120C, NVPS@NC-2 cathode still exhibits 98.9/66.2 mAh g −1 , maintaining 92.9% and 90.6% capacities after 5000/10000 cycles, respectively, showing its viability for advanced SIBs applications.
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2026
·
IF 2.5
Chunhui Shan,Qi Bing,Gang Qiu,Hui Zhang,Yingmin Chen
Galectin-3 disrupts microglial P2X7R/MerTK balance after stroke • ERK/ROS/BRD4 axis mediates Gal-3-induced P2X7R upregulation • Gal-3 promotes cGAS-STING inflammation and apoptosis via HIF1α/P2X7R • TD-139 restores P2X7R/MerTK balance and reduces cerebral infarction • Targeting Gal-3 improves motor, cognitive, and grip strength in MCAO rats This study demonstrates that Galectin-3 (Gal3) exacerbates cerebral infarction progression by modulating the P2X7R/MerTK equilibrium in microglia via the ERK/BRD4/HIF1α signaling axis, with reactive oxygen species (ROS) serving as a potential intermediate mediator linking ERK activation to BRD4 upregulation based on prior literature evidence. Single-cell transcriptomic analysis revealed that ischemia drives microglia from a homeostatic state to an activated disease phenotype, and virtual Gal3 knockout predicted corresponding downregulation of BRD4. Using a rat model of cerebral infarction, we found that the Gal3 inhibitor TD-139 significantly reduced infarct volume, promoted M2 polarization of microglia, and improved neurological function in behavioral assays. Mechanistic investigations in BV2 microglia revealed that Gal3 activates ERK1/2, leading to ROS accumulation, BRD4 upregulation, and subsequent HIF1α-mediated P2X7R transcription, while suppressing KLF4 and MerTK. This cascade increased P2X7R expression and suppressed MerTK/KLF4 expression, generating a pro-inflammatory and pro-apoptotic microenvironment. Notably, pharmacological inhibition of Gal3 enhanced efferocytosis and reduced apoptosis, whereas P2X7R blockade exerted similar neuroprotective effects. These findings identify Gal3 as a key regulator of microglial polarization via the experimentally validated ERK/BRD4/HIF1α-P2X7R signaling cascade, where P2X7R activation concurrently triggers cGAS-STING-mediated pro-inflammatory/pyroptotic responses and suppresses the KLF4/MerTK efferocytosis axis. Targeting this regulatory network could provide therapeutic benefits in cerebral infarction by shifting microglia toward the neuroprotective M2 phenotype and facilitating inflammation resolution. Targeting this pathway could provide therapeutic benefits in cerebral infarction by shifting microglia toward the neuroprotective M2 phenotype and facilitating inflammation resolution. Data were analysed using one-way ANOVA followed by Tukey's post-hoc test for multiple comparisons; P < 0.05 was considered statistically significant.
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高影响因子
Biosensors-Basel
·
2025
·Vol. 15
·期 6
·354
·
IF 5.6
Fengli Gao,Lin Liu,Cancan He,Yong Chang,Weiqiang Wang
Nanoquenchers with a single quenching cofactor exhibit limited fluorescence quenching efficiency. In this work, a metal–organic hybrid with dual quenching cofactors (Cu2+and pyrroloquinoline quinone or PQQ) was prepared by metal-coordinated assembly and used as a nanoquencher for a protease assay with enhanced quenching efficiency. The peptide substrate with an oligohistidine (His6) tag was labeled with a fluorophore. Caspase-3 was determined as a protease example. The substrate was attached onto the surface of the Cu-PQQ nanoquencher by a metal coordination interaction between the unsaturated Cu2+on the nanoparticle surface and the His6tag in the peptide. The cleavage of the peptide substrate by enzymatic hydrolysis led to the release of a fluorophore-conjugated segment from the nanoquencher surface, thus turning on the fluorescence. The nanoprobe was used to determine caspase-3 with a linear range of 0.01–5 ng/mL and a detection limit of 7 pg/mL. Furthermore, the method was used to evaluate inhibition efficiency and monitor drug-induced cell apoptosis. In contrast to other means of peptide immobilization, such as physical adsorption and covalent coupling, the strategy based on the metal coordination interaction is simple and powerful, thereby achieving assays of caspase-3 activity in lysates with a satisfactory result. The work should be valuable for the design of nanoquenchers with multiple quenching cofactors and the development of novel biosensors.
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DOI
PubMed
高影响因子
Sensors and Actuators Reports
·
2025
·
IF 7.6
Yuhuai Qin,Wenxue Sun,Joseph Brake,Sulin Gao,Qianqian Li,Xinrong Zhu,Jiang Bian,Xiaobin Wu
Lead ions are an environmental pollutant and a threat to human health. Traditional methods for detecting lead ions suffer disadvantages such as being time-consuming and expensive, therefore there is a need to develop simpler and faster methods. We developed an optimized spectroscopic method to detect lead ions by first identifying a lead-specific chelator 2,3-dimercaptosuccinic acid (DMSA) which was screened using a novel biological screening system and verified by metal chromophore assays. Synthetic gold nanostars (GNSs) were explored for use as probes and we found that DMSA-Pb chelates catalyzed the etching of GNSs in solution resulting in a color change. We observed a change in the longitudinal local surface plasmon resonance (LSPR) peak of GNSs in the presence of DMSA-Pb which could be measured in subsequent experiments. The optimized etching reaction displayed strong sensitivity and selectivity for Pb 2+ and achieved a detection limit of 2 nM less than 2 minutes, which is well below the safety threshold of Pb 2+ in environmental water and food. The Pb 2+ contents of rice, beans, milk, grapes, peaches, and cabbage were detected by the DMSA-Pb GNSs method and were consistent with those of inductively coupled plasma mass spectrometry (ICP-MS). Our study provides a novel and practical approach for rapid, safe, and cost-effective detection of lead ions.
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高影响因子
iScience
·
2023
·
IF 5.8
Shi-Jun Wang,Long Wang,Xiang-Ying Tang
B(C 6 F 5 ) 3 ·H 2 O has been long recognized as a common Brønsted acid. The lack of X-ray crystal structure of B(C 6 F 5 ) 3 ·H 2 O with other substrates has greatly limited the development of a new catalytic mode. In this work, a complex of B(C 6 F 5 ) 3 ·H 2 O and amide 2-phenyl-3,4-dihydroisoquinolin-1( 2H )-one with hydrogen bonds and π-π interactions is characterized by X-ray diffraction. Such noncovalent interactions in solution also exist, as verified by NMR, UV-Vis absorption, and fluorescence emission measurements. Moreover, the mixture of amide 2-phenyl-3,4-dihydroisoquinolin-1( 2H )-one and B(C 6 F 5 ) 3 ·H 2 O, instead of other tested Brønsted acids, shows a tailing absorption band in the visible light region (400–450 nm). Based on the photoactive properties of the complex, a photoredox catalysis is developed to construct α -aminoamides under mild conditions.
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高影响因子
POLYMER
·
2026
·
IF 5.1
Chang Liu,Yuming Cui,Yunbo Mo,Qian Xu,Kai Wang,Jianwu Lan,Shaojian Lin
Commercial poly(butylene adipate-co-terephthalate) (PBAT), a biodegradable aromatic–aliphatic copolyester, is considered a promising alternative to conventional plastics in various fields. However, its relatively low mechanical strength and low glass transition temperature (T g ) often restricts broader applications. In this study, bio-based 1,3-propanediol (PDO) was employed to completely replace 1,4-butanediol in PBAT, and a series of poly(trimethylene terephthalate- co -trimethylene adipate) (PPAT) copolyesters were synthesized via melt polycondensation. Structural characterization confirmed the targeted copolyester compositions and random chain segments (R ≈ 1), with intrinsic viscosities of 0.89-1.01 dL/g. Crystallographic analysis revealed that the crystallinity of PPATs varied with the adipic acid/dimethyl terephthalate molar ratio while preserving a monoclinic crystal structure. Notably, PPAT55 exhibited a significantly higher T g (−7.8 °C) than PBAT (−26.1 °C), and all copolyesters showed good thermal stability for melt processing. Importantly, PPAT55 fibers achieved a tensile strength of 0.71 cN/dtex after threefold drawing, representing a 51.06% enhancement over PBAT fibers. Furthermore, the increased aliphatic segment content promoted by hydrolytic susceptibility, as demonstrated by enzymatic degradation tests, with PPAT55 showing a 9.93% mass loss after 42 days, demonstrating satisfactory biodegradability comparable to PBAT. The degradation mechanism was further elucidated at the molecular level through theoretical calculations. This work provides a feasible strategy for developing high-performance sustainable polyester materials with good spinnability.
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高影响因子
DESIGNED MONOMERS AND POLYMERS
·
2014
·
IF 2.21
Bo Gao,Ping Guan,Xiaoling Hu,Liwei Qian,Qiaoli Wang,Longfei Yang,Dan Wang
L-Phenylalanine (L-Phe) imprinted polymers (L-MIPs) were prepared via precipitation polymerization using 1-vinyl-3-carboxymethylimidazolium chloride ([VCIM]Cl) as functional monomer, which could interact with L-Phe through electrostatic interactions, hydrogen-bonding, and π–π interactions. The morphologies of obtained polymers were observed by a scanning electron microscope, and their structures were analyzed by Fourier transform infrared spectroscopy. The effects of monomer species and molar ratio of template to functional monomer or crosslinker on adsorption capacity and imprinting factors (IFs) of polymers were investigated. The result indicated that [VCIM]Cl was preferable monomer and the optimum molar ratio of template to functional monomer and crosslinker was 1:4:16. The maximum IF of the obtained L-MIPs could reach 3.86 in 0.4 mg mL−1 L-Phe solution at 25 °C, after 8 h of adsorption and the maximum adsorption capacity of L-MIPs for L-Phe was 33.08 mg g−1. The adsorption behavior of L-Phe on the L-MIPs was construed seriously within the experimental concentration by Freundlich model and Langmuir model. In terms of the higher correlation coefficient, it was considered that the adsorption behavior of L-Phe fit Freundlich model better than Langmuir model, which means that the distribution of active sites was heterogeneous in L-MIPs. The selectivity adsorption showed L-MIPs have better specificity and selectivity for L-Phe than that of L-His and L-Trp. Finally, L-MIPs were applied to the CD and Automatic Polarimeter experiments, showing that L-MIPs had the capability of specific chiral recognition for L-Phe.
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危险品化学品经营许可证（带存储）