SENSORS AND ACTUATORS B-CHEMICAL
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2020
·
IF 7.1
Peipei Wang,Tian Liu,Yena Liu,Jing Tian,Xinyu Zhang,Jiaqi Guo,Yongcan Jin,Huining Xiao,Junlong Song
It is a challenge to interpret the signals obtained by Quartz Crystal Microbalance (QCM) and Surface Plasmon Resonance (SPR) accompanied with in the significant changes of the density and/or refractivity of media. The work presented herein focuses on demonstrating a universal in-situ and real-time monitoring methodology for ultrathin film formation on gold chips in varied media. Biomimetic model cellulase/enzyme film was immobilized in-situ on gold surface of sensors, followed by revealing its interaction with lignosulphonate in the media changed from ethanol-based to aqueous ones. Combining the techniques of Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) and Multi-parametric Surface Plasmon Resonance (MP-SPR) along with respective software and models enabled us to precisely characterize the thickness, viscous and shear elastic moduli, and the coupled solvent for individual layer formed over the entire process, which are unachievable with conventional methods. The methodology provides real-time monitoring of filming process and offers us an in-situ qualify control approach to the biomimetic model films formed on different substrates including gold chip.
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JOURNAL OF CATALYSIS
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2020
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IF 7.888
Chenyuan Guo,Shuguang Shen,Meina Li,Ying Wang,Jing Li,Yuanquan Xing,Cui Wang,Huajie Pan
A rapid and efficient synthesis strategy of MgAl-LDH was proposed. MgAl-LDH with high specific surface area was synthesized in situ by using η -Al 2 O 3 as carrier, and the synthesis time was greatly shortened by both increasing temperature and introducing ethanol as co-solvent. Besides, the growth process of MgAl-LDH on the surface of η -Al 2 O 3 is also revealed. Under optimized conditions, the specific surface area of the MgAl-LDH is as high as 172.4 m 2 /g, the crystalline size is as small as 12.82 nm, and the basicity can reach 1.795 mmol/g. The urea wastewater was degraded from 8000 mg/L to 6.85 mg/L over the catalyst synthesized by the rapid method, and the catalyst still maintains high activity after four uses. Also, it was found that there is a good linear relationship between the urea removal rate and the basicity of MgAl-LDH.
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Journal of Environmental Sciences
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2021
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IF 5.565
Xueyi Dong,Xihua Cao,Wenbin Jiang,Xiuxian Song,Zhiming Yu,Shitao Yu
Polyaluminum chloride modified clay (PAC-MC) is a safe and efficient red tide control agent that has been studied and applied worldwide. Although it is well known that the distribution of hydrolytic aluminum species in PAC affects its flocculation , little is known about the influence of particulars aluminum species on the microalgae removal efficiency of PAC-MC; this lack of knowledge creates a bottleneck in the development of more efficient MCs based on aluminum salts. The ferron method was used in this study to quantitatively analyze the distributions of and variations in different hydrolytic aluminum species during the process of microalgae removal by PAC-MC. The results showed that Al a , which made up 5%–20% of the total aluminum, and Al p , which made up 15%–55% of the total aluminum, significantly affected microalgae removal, with Pearson's correlation coefficients of 0.83 and 0.89, respectively. Most of the aluminum in the PAC-MC sank rapidly into the sediments, but the rate and velocity of settlement were affected by the dose of modified clay. The optimal dose of PAC-MC for precipitating microalgae was determined based on its aluminum profile. These results provide guidance for the precise application of PAC-MC in the control of harmful algal blooms .
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PubMed
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Frontiers in Medicine
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2021
·
IF 5.093
Li Yujuan,Zhang Wencui,Sun Tainning,Liu Baowen,Manyande Anne,Xu Weiguo,Xiang Hong-Bing
The high incidence of patients with chronic itch highlights the importance of fundamental research. Recent advances in the interface of gut microbiota have shed new light into exploring this phenomenon. However, it is unknown whether gut microbiota plays a role in chronic itch in rodents with or without cognitive dysfunction. In this study, the role of gut microbiota in diphenylcyclopropenone (DCP)-evoked chronic itch was investigated in mice and hierarchical cluster analysis of novel object recognition test (ORT) results were used to classify DCP-evoked itch model in mice with or without cognitive dysfunction (CD)-like phenotype and 16S ribosomal RNA (rRNA) gene sequencing was used to compare gut bacterial composition between CD (Susceptible) and Non-CD phenotypes (Unsusceptible) in chronic itch mice. Results showed that the microbiota composition was significantly altered by DCP-evoked chronic itch and chronic itch induced novel object recognition-related CD. However, abnormal gut microbiota composition induced by chronic itch may not be correlated with novel object recognition-related CD.
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PubMed
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COMPOSITES SCIENCE AND TECHNOLOGY
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2020
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IF 7.094
Shengjie Guan,Zhengxiang Zhong,Jun Li,Yaoyuan Xu,Lei Ding,Yudong Huang,Li Liu
Dithiocarbamate binding to nickel was successfully synthesized on the surface of carbon fibers acting as the catalyst of carbon nanotubes via two feasible strategy, including typical one–step method with metal hydroxide and green mild Markovnikov addition procedure using ethyl vinyl ether. The positive effects of modified carbon fibers were investigated, consisting of microstructure, roughness, and surface energy, etc. Further, carbon fiber composites with carbon nanotubes grown in-situ were prepared by combining methylphenyl silicone and phenolic resin , then undergoing a hyperthermal sintering process . The surface morphologies proved that the microstructure of composites changed dramatically due to substantial carbon nanotube whiskers. Furthermore, the mechanical performance of high temperature treated composite materials with higher modulus and strength had been enhanced, which the compressive strength and interlaminar shear strength could reach approximately 20 MPa (further up to 150%) and 10 MPa (120%). The modification of carbon fibers and growth of CNTs in-situ would contribute to competitive application of resin-based composites in the field of thermal bearing and insulation.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2020
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IF 3.721
Zhou-Tao Fang,Wen-Ting Yang,Cun-Yu Li,Da Li,Jun-Jie Dong,Dong Zhao,Hai-Rong Xu,Jian-Hui Ye,Xin-Qiang Zheng,Yue-Rong Liang,Jian-Liang Lu
Flavonoids are important phenolic compounds in tea plant since they have a large number of physiological functions for plants themselves and human beings. Tea samples (n = 151) harvested from 3 varieties, 9 plantation areas and 107 cultivars were analyzed to describe the attributes of catechins and flavonol glycosides in China. A support vector machine (SVM) classifier was built to recognize the assamica variety with 94.2 % accuracy. Accumulation of (-)-epicatechin gallate (ECG) and flavonol di-glycosides was especially observed in assamica variety, while (-)-epigallocatechin gallate (EGCG) and flavonol tri-glycosides were obviously accumulated in sinensis variety. The ratios of ECG/EGCG and flavonol tri-glycosides/flavonol di-glycosides could be used as indicators for distinguishing the different varieties and cultivars. Accumulation pattern of catechins and flavonol glycosides were mainly dependent on the genetic background of the cultivars rather than plantation locations. The increased hydroxyl groups at the C-ring of catechins and the extended sugar chain at the B-ring of flavonol glycosides could be considered as important rules of metabolite change during tea plant evolution. The mechanism of accumulation of flavonoids in various varieties and cultivars was also discussed.
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Analytical Methods
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2020
·Vol. 12
·期 17
·2221-2227
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IF 2.596
Junjie Du,Qiwei Zhang,Jianjun Li,Qi Zheng
Sialic acid (Sia) is widespread in vertebrates and humans as well as in some microbes and invertebrates. It is well known that O-acetylation is a common modification for Sia. Also, highly O-acetylated Sias have been reported in humans, bovines, and fish. Derivatization based on 1,2-diamino-4,5-methylenedioxybenzene (DMB) coupled with LC-fluorescence detection or LC-MS analysis has been the routine analytical strategy for O-acetylation profiling of Sias. Unfortunately, DMB offers poor chromatographic resolution for some O-acetylated species. Herein, we report another derivatization reagent, 4,5-dimethylbenzene-1,2-diamine (DMBA), for the LC-MS analysis of Sia isomers. The DMBA method was first evaluated using Sia reference standard, horse serum and carp blood. The results demonstrate that DMBA provides superior chromatographic separation efficiency and comparable MS/MS spectra to DMB. The proposed method was then applied to the analysis of relative abundance of each Sia species in fish tissues, including liver, intestine, muscle and brain. It was found that the relative amounts of Sia species were significantly different among the four tissues. Thus, DMBA derivatization-based LC-MS methods have great potential in investigating the O-acetylation level of Sias in various samples, such as human food and bio-pharmaceutical products.
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Molecular Catalysis
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2020
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IF 3.687
Caixia Cui,Hong Ming,Linjing Li,Mingjie Li,Jian Gao,Tao Han,Yunyun Wang
We report here adopting a glucose template for synthesizing mesoporous silica for enzyme in-situ immobilization. Bifunctional GSH synthetase (GSHF) and polyphosphate kinase (PPK) were selected as model system for synthesizing GSH with ATP regeneration. Results indicated that a pore size that was slightly smaller than that of enzyme was responsible for the high performance of immobilized enzyme inside mesoporous without protein leaking. Then, the cationic dopamine was used for PPK immobilization by self-polymerization. The co-immobilized enzyme activity was much higher than that of the free enzyme. The catalytic efficiency kcat/Km of co-immobilized enzyme was higher than that of the free enzyme (8.5 and 1.5 times higher for GSHF and PPK, respectively). After optimizing the reaction parameters, the co-immobilized enzymes exhibited excellent operational and storage stability, and could be re-used seven times and retained the original activity after 30 days. It is hoped that these findings will pave the way for applying enzyme co-immobilization to biocatalytic system.
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JOURNAL OF FOOD PROCESSING AND PRESERVATION
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2020
·Vol. 44
·期 4
·e14383
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IF 1.405
Zi-qiang Liu,Da-yong Zhou,Yu-xin Liu,Xiao-yang Liu,Yan Liu,Bing Liu,Liang Song,Fereidoon Shahidi
Matrix metalloprotease (MMP) is the key endogenous protease that boosts the autolysis of sea cucumber, while its action and mechanism in vivo are still unclear. MMP inhibitor (1,10-phenanthroline) was injected into coelomic cavity of sea cucumber ( Stichopus japonicus ) and its effects on autolysis and mechanism of action were investigated for the first time by using scanning electron microscopy, multi-functional in vivo imaging system, and confocal laser scanning microscopy. In response to external stimuli, the level of Ca 2+ in live sea cucumber dermis increased, which may activate MMP activity. MMP inhibitor could diffuse from coelomic cavity into body wall and significantly prevented the activation of MMP by chelating Ca 2+ , and thus, retarded the autolysis of fresh sea cucumber by preventing degradation of interfibrillar proteoglycan bridges between adjacent collagen fibrils. This work provides information toward understanding the mechanism behind the sea cucumber autolysis and demonstrate a potential approach to prevent the autolysis. Practical applications Autolysis occurs in sea cucumber in response to external stimulation. Matrix metalloproteinase (MMP) has been found to be the key endogenous protease that boosts the autolysis of sea cucumber body wall (SCBW). However, the role of MMP in the autolysis of SCBW in vivo and the mechanisms involved remain unclear. In this study, MMP inhibitor (1,10-phenanthroline) was injected into the body cavity of live sea cucumber, and its effects on in situ changes in SCBW were studied. Our results indicated that the activity of endogenous MMP in live SCBW was activated in response to external stimulation, which may be responsible for the autolysis. After injection into coelomic cavity, MMP inhibitor could diffuse into SCBW and significantly reduced the progress of typical morphological and microstructural changes of autolytic sea cucumber. Through chelating Ca 2+ , the MMP inhibitor could prevent the activation of MMP, and thus reduced the autolysis of fresh sea cucumber.
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BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
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2020
·
IF 1.186
Guosong Xin,Miao Yu,Yang Hu,Shiyong Gao,Zheng Qi,Yuan Sun,Wenjing Yu,Jiaxin He,Yubin Ji
Lycorine is an isoquinoline alkaloid in Amaryllidaceae with antitumor, antibacterial, antiviral, diuretic and cardiovascular effects. This study explored the antitumor mechanism of lycorine in vitro and in vivo by focusing on its effects on tumor cell membrane fraction and tumor cell membrane structure. The results showed that lycorine had antitumor effect on H22 tumor-bearing mice in vivo and could effectively prolong the survival time of H22 tumor-bearing mice. In vitro, lycorine inhibited the proliferation of HepG-2 cells and induced cell death significantly. Lycorine reduced the total protein content, sialic acid content and cholesterol content on the surface of tumor cell membrane, and reduced the content of some main components of tumor cell membranes. Lycorine reduced the membrane fluidity and cell membrane integrity of tumor cells. In addition, the ion channel (Na+, K+-ATPase, Ca2+, Mg2+-ATPase) activity on the surface of the tumor cell membrane decreased. This contributed to a significant antitumor effect in vitro and in vivo.
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JOURNAL OF HAZARDOUS MATERIALS
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2020
·
IF 9.038
Y.L. Zhao,G.G. Li,H.M. Hou,J.C. Shi,S.N. Luo
We investigate atomic and molecular emission of laser-ablated nitromethane in an Ar or N 2 buffer gas, with fs laser-induced breakdown spectroscopy. The electronic bands of CN, C 2 , and NH molecules and the atomic transition lines of C I, N I, and H α are identified. The time series of the emissions are obtained, and the formation mechanisms of CN and C 2 are deduced. The CN violet system, the B 2 Σ + -X 2 Σ + (0-0) band, is chosen to extract plasma temperature from the experimental spectra.
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PubMed
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BIOLOGICAL TRACE ELEMENT RESEARCH
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2020
·Vol. 198
·期 1
·58-67
·
IF 2.639
Wang Hetao,Liu Hao,Zhou Meijuan,Shi Hanping,Shen Mei
We established a method of determining 13 trace elements (TEs) in whole blood, determined the levels of the TEs in patients with colorectal cancer (CRC), and examined the correlations between the TEs and circulating tumor cells (CTCs). A total of 93 CRC patients and 48 control subjects in Guangzhou, China, were included. Inductively coupled plasma mass spectrometry (ICP-MS) was used to measure the blood levels of 13 TEs. CTCs of 13 CRC patients were examined using CanPatrol™. The levels of Mg, Fe, Zn, Se, Sr, Mo, and Ba in CRC patients were lower than those in control subjects (all, P < 0.05). The levels of Mg, Fe, Zn, Se, and Ba decreased with increasing TNM stage. The reductions of Zn and Se levels were higher in early-stage than late-stage CRC patients. The levels of Cu and Ni were higher in CRC patients than in control subjects (both, P < 0.05). The Cu level increased with increased TNM stage, and Ni level was higher in early-stage CRC patients as compared with late-stage CRC patients. Consequently, the TE levels showed differences as the process of TNM stage of CRC patients. There were correlations between the amount of CTCs and epithelial-CTCs (E-CTCs) and interstitial-CTCs (M-CTCs) ( P < 0.05). The levels of Mg, Cr, Fe, Cu, Zn, Se, Sr, and Ba were correlated with the number of CTCs ( P < 0.05). Specific TE patterns in whole blood may assist in the early diagnosis of CRC. The combined determination of TE levels and CTCs may be useful as an adjunct to staging and guiding treatments.
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