INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Zhentao Chen,Lijing Lei,Xiangze Fan,Jinhua Zhu,Haodong Zhou,Yun Zhang,Fanyi Ma,Xiuhua Liu,Md. Zaved H. Khan
ZIF-8 as multi-dentate crosslinking hub converts weak CS/γ-PGA complexes into robust dual-network hydrogel. • Ultra-large pores eliminate diffusion limitation (Da ≈ 0.001) with K m increase of only 2.2-fold. • HSP regression (R 2 = 0.76, p ≈ 0.0002) validates synergistic thermodynamic exclusion and size-sieving mechanism. • Immobilized α-Glu@PC/ZIF-8 enables ligand fishing of two α-glucosidase inhibitors via capture-wash-elute workflow. Herein, a composite hydrogel based on two natural macromolecules—chitosan (CS) and γ-poly(glutamic acid) (γ-PGA)—was developed, crosslinked by zeolitic imidazolate framework-8 (ZIF-8) nanoparticles, which serve as reactive crosslinking hubs to covalently connect the biopolymer chains for efficient immobilization of α-glucosidase (α-Glu). A carboxyl-terminated poly(ethylene glycol)–poly(glutamic acid) copolymer was first synthesized and its activated carboxyl groups underwent amidation with amino groups of CS to rapidly form a three-dimensional PC/ZIF-8 hydrogel. The effects of ZIF-8 content and CS concentration on micro-morphology, swelling behavior, and rheological properties were systematically investigated. ZIF-8-mediated crosslinking was essential for gel formation, enabling effective modulation of network density and pore architecture. The optimized hydrogel showed no inhibitory effect on α-Glu. Immobilized α-Glu@PC/ZIF-8 exhibited enhanced pH stability (retaining 53% and 70% activity at pH 4.0 and 9.0), outstanding storage stability (>90% after 4 weeks), excellent organic solvent tolerance (>90% in most solvents), and good reusability (near 100% activity over 6 cycles). Mass transfer studies confirmed that the designed crosslinking density provided efficient substrate/product diffusion channels, minimizing product inhibition. Hansen solubility parameter analysis revealed a synergistic “thermodynamic exclusion + size-sieving” mechanism for solvent tolerance. Coupled with HPLC, the immobilized enzyme successfully identified two α-glucosidase inhibitors—batatasin I and 2,4-dimethoxy-6,7-dihydroxyphenanthrene—from Dioscorea opposita Thunb. peel. This work demonstrates that rational chemical modification of natural macromolecular networks via MOF crosslinkers provides an effective strategy for constructing high-performance enzyme immobilization platforms. Download: Download high-res image (72KB) Download: Download full-size image
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Xiaona Cao,Gailing Tang,Xing Fan,Xinyu Gao,Tianxin Wang,Xiaohan Zhang,Wenjie Wang,Hao Wang
Establishing a chitosan microsphere-immobilized α-amylase system. • Identifying procyanidin B 2 as the core α-amylase inhibitory component within apple polyphenols. • Elucidating the inhibitory mechanism of procyanidin B 2 through multi-technique integration. Inhibition of α-amylase (PPA) delays starch digestion and glucose absorption, serving as an effective means to regulate postprandial hyperglycemia. Apple polyphenols (APE) exhibits PPA inhibitory activity, but their core active components and mechanisms of action remain unclear. This study employed immobilized enzyme coupled with HPLC-Q-TOF-MS/MS to screen PPA inhibitors from APE and elucidate their mechanisms of action.Using amino-modified chitosan microspheres as carriers, PPA was covalently immobilized via genipin-mediated Schiff base reaction. The optimal immobilization conditions were optimized (crosslinking agent concentration 0.17%, enzyme concentration 1 mg/mL, crosslinking 6 h, immobilization 14 h), establishing a stable screening system. APE was fractionated into 9 components by solvent precipitation, with component F 3 exhibiting the most potent inhibitory activity. Based on the aforementioned techniques, 4 active compounds were identified from F 3 , and validation confirmed that procyanidin B 2 (PC B 2 ) demonstrated the most effective inhibitory effect. In vitro experiments and multi-technology validation demonstrated that PC B 2 could delay starch hydrolysis and reduce the production of reducing sugars to regulate postprandial blood glucose, exhibiting reversible competitive inhibition against PPA (IC 50 = 0.25 mg/mL). It could bind to PPA and alter its secondary and tertiary structures, inducing intrinsic protein fluorescence quenching through static quenching. The binding of the two components triggered an exothermic reaction, forming a stable complex. Download: Download high-res image (140KB) Download: Download full-size image
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Food Chemistry-X
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2026
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IF 8.2
Mingyu Zhu,Zhiyun Peng,Guangcheng Wang
The fruit of Rosa roxburghii Tratt. (RRT) is frequently eaten in Guizhou Province, China. Thirty compounds from RRT fruit were identified by the bioaffinity-ultrafiltration method. Among them, quercetin, diosmetin, luteolin, and apigenin (IC 50 = 4.80 ± 0.12 μg/mL, 52.62 ± 0.26 μg/mL, 17.51 ± 0.27 μg/mL, 33.24 ± 0.32 μg/mL) exhibited significant α-glucosidase inhibitory activities. Based on multispectral and molecular docking analyses, these active compounds bind to the active site of α-glucosidase via at least four hydrogen bonds and van der Waals forces, thereby altering its hydrophobic environment and secondary structure. in vivo studies showed that compared to the AUC of postprandial glucose in the model (2809.33 ± 64.84 mM·min) and in the acarbose group (2525.40 ± 54. 28 mM·min), these compounds decreased postprandial blood glucose levels. Long-term administration of apigenin additionally improved lipid metabolic disorders. These results support the development of RRT fruit as a hypoglycemic functional food.
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Journal of Physical Chemistry C
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2013
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IF 4.814
X. Ge,C. D. Gu,X. L. Wang,J. P. Tu
An ionothermal strategy is developed to synthesize hydrotalcite-like α-Co(OH)2 sheets. The structure and interlayer chemistry of α-Co(OH)2 are facilely manipulated, thus determining its pseudocapacitive performance. Significantly, a very large space of 11.2 Å in (003) plane induced by various intercalated species is found in the ionothermal synthesized α-Co(OH)2 obtained at 210 °C (210-α-Co(OH)2), which delivers a better pseudocapacitive performance. Annealing the synthesized α-Co(OH)2 generates mesoporous Co3O4 sheets or nanoparticles, which depends on the structure of the precursors. More defects in the α-Co(OH)2 precursor provide larger driving force during the nucleation of Co3O4 nanocrystals, thus reducing the grain size. The architecture of the 210-Co3O4 derived from 210-α-Co(OH)2 is featured as self-supporting mesoporous nanosheets composed of ∼5 nm sized grains. The fine grains and ultralarge specific surface area of ∼113.5 m2 g–1 in the 210-Co3O4 can trigger the formation of CoO intermediate during lithium ion insertion into the Co3O4. The 210-Co3O4 exhibits superior cycling performance as electrode for lithium ion battery and supercapacitor over the other Co3O4 samples due to its nanocrystallite constructed mesoporous sheet structure, which has a higher strain accommodation capability. The underlying mechanism discussed in this work provides guidance on structural design of advanced energy storage materials.
研究领域:
材料科学
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电化学
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储能
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纳米材料
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无机合成
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APPLIED SURFACE SCIENCE
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2026
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IF 6.6
Yingying Chen,Zhihua Liu,Jinshan Liu,Miao Zhou,Zhifeng Liu
Tetracycline hydrochloride (TCH) has emerged as a pressing environmental issue due to their extensive use and recalcitrant nature. The integration of Fenton reaction with photoelectrocatalysis, forming a photoelectro-Fenton system, is emerging as a promising strategy for advanced water treatment. This study aims to address the critical challenges of severe charge recombination and sluggish surface reaction kinetics in α-Fe 2 O 3 during photoelectrocatalytic pollutant degradation. Through Co-doped modification, Fe 2−x Co x O 3 photoanodes were successfully fabricated. The results demonstrate that partial substitution of Fe 3+ by Co 2+ not only effectively enhances charge carrier concentration but also induces the formation of substantial oxygen vacancies. This synergistic effect consequently leads to significantly improved electrical conductivity and expanded visible-light absorption range. Firstly, Co 2+ functions as an electron donor, continuously reducing Fe 3+ to Fe 2+ and thereby sustaining the cycling of the Fe 3+ /Fe 2+ . Concurrently, this process facilitates the efficient and continuous conversion of oxygen to H 2 O 2 via a two-electron reduction pathway. Secondly, the coexisting Co 2+ /Co 3+ and Fe 2+ /Fe 3+ bimetallic cycles on the material surface enabling sustained activation of H 2 O 2 to trigger the Fenton reaction, which generates substantial amounts of highly reactive hydroxyl radicals (·OH) and other active substances, achieving rapid decomposition and mineralization of TCH molecules.
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EUROPEAN POLYMER JOURNAL
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2025
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IF 6.3
Qidi Hu,Cheng Cao,Hanglin Li,Jian Xu,Sheng Han,Jiusheng Li
The performance demands of mechanical lubricants are intrinsically linked to the operational reliability, efficiency, and service life of machinery, encompassing critical aspects such asextreme condition adaptability, long-term stability, and environmental compatibility. However, conventional lubricants exhibit limitations in fulfilling these multifaceted operational requirements. In this study, we developed a bifunctional metallocene-catalyzed polyalphaolefin (mPAO) base oil integrating antioxidant and antiwear characteristics. Two sulfur-phosphorus additives—diphenyldithiophosphoric acid phenyl ester (DPDTP) and butyl phosphorothioate derivative (BPSD)—were synthesized. A series of functionalized mPAO alkylation products were successfully prepared by grafting selected organophosphorus compounds onto mPAO via alkylation. Oxidation stability was assessed through pressurized differential scanning calorimetry (PDSC) and thermogravimetric analysis (TGA). Compared with mPAO, m-DPDTP demonstrated remarkable antioxidant enhancement, exhibiting a 74 °C higher initial oxidation temperature and a 61.6 °C increase in onset decomposition temperature. Tribological performance was systematically investigated using four-ball wear tests, UMT tribometer measurements, and TE77 reciprocating friction evaluations. The m-DPDTP exhibited reductions in average friction coefficients of 35.91 %, 56.85 %, and 47.78 %. Additionally, a 56.85 % reduction in wear scar diameter was observed during the four-ball evaluations. Additionally, m-DPDTP demonstrated the highest PB (212 N) and PD (250 N). These findings substantiate that DPDTP-grafted alkylation products possess superior friction reduction, antiwear capabilities, and extreme-pressure performance. This work establishes a molecular engineering strategy combining structural design and composite modification to develop mPAO alkylation products with concurrent thermal-oxidative stability and tribological enhancement. This breakthrough overcomes the functional singularity of conventional base oils and provides a novel paradigm for advanced lubricant formulation.
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CARBOHYDRATE POLYMERS
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2023
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IF 11.2
Yixi Liu,Yazhen Wu,Hangyan Ji,Xiaoxiao Li,Zhengyu Jin,Birte Svensson,Yuxiang Bai
Isomalto/malto-polysaccharides (IMMPs) derived from malto-oligosaccharides such as maltoheptaose (G7) are elongated non-branched gluco-oligosaccharides produced by 4,6-α-glucanotransferase (GtfB). However, G7 is expensive and cumbersome to produce commercially. In this study, a cost-effective enzymatic process for IMMPs synthesis is developed that utilizes the combined action of cyclodextrinase from Palaeococcus pacificus (PpCD) and GtfB-ΔN from Limosilactobacillus reuteri 121 to convert β-cyclodextrin into IMMPs with a maximum yield (16.19 %, w/w). The purified IMMPs synthesized by simultaneous or sequential treatments, designated as IMMP-Sim and IMMP-Seq, possess relatively high contents of α-(1 → 6) glucosidic linkages. By controlling the release of G7 and smaller malto-oligosaccharides by PpCD, IMMP-Seq was obtained of DP varying from 12.9 to 29.5. Enzymatic fingerprinting revealed different linkage-type distribution of α-(1 → 6) linked segments with α-(1 → 4) segments embedded at the reducing end and middle part. The proportion of α-(1 → 6) segments containing the non-reducing end was 56.76 % for IMMP-Sim but 28.98 % for IMMP-Seq. Addition of G3 or G4 as specific acceptors resulted in IMMPs exhibiting low polydispersity . This procedure can be applied as a novel bioprocess that does not require costy high-purity malto-oligosaccharides and with control of the average DP of IMMPs by adjusting the substrate composition .
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Journal of Cleaner Production
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2023
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IF 11.1
Xiunan Cai,Qingling Liu,Qing Yang,Fuzheng Fu,Yanjuan Zhang,Tao Gan,Huayu Hu,Zuqiang Huang
To effectively utilize the hazardous industrial solid waste manganese residue (MR), a MR-derived Fe 2 O 3 -baesd photocatalyst with efficient charge transfer, abundant reactive sites and high redox performance was developed for simultaneously removing organic pollutants and heavy metals . Herein, a novel “confined growth” strategy was proposed to fabricate an amorphous MnO 2 decorated α-Fe 2 O 3 @geopolymer sphere (A-MnO 2 /Fe 2 O 3 @GS) Z-scheme heterojunction by ion exchange method, which was used as a photo-Fenton catalyst for efficiently reducing Cr(VI) and degrading ciprofloxacin (CIP). Characterization results reveal that the 3D hydrangea-like MnO 2 with low crystallinity could not only capture and accelerate electron transfer but also act as an electron conduction bridge and a surface oxygen adsorption site, resulting in efficient separation and utilization of charge. More importantly, the photocatalytic reduction capacity was greatly enhanced in Z-scheme heterojunction owing to the lower conduction band potential of MnO 2 . A-MnO 2 /Fe 2 O 3 @GS showed excellent photocatalytic activity with CIP removal of 99.1% within 40 min and Cr(VI) removal of 97.6% within 50 min, which were 8.13 and 3.47 times higher than those catalyzed by Fe 2 O 3 @GS, respectively. This can be ascribed to the formation of intimate interface, internal electrostatic field , and rich structure defects in the Z-scheme heterojunction. This work offers a new idea to construct Z-scheme photocatalysts with high reactivity for environmental modification.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2023
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IF 8.6
Lili Bo,Fang Nian,Lumei Pu,Ping Li,Yusen Hu,Jinhui Tong
Hybrids of α-Fe 2 O 3 /Fe 3 O 4 nanoparticles embedded in N doped graphitic carbon polyhedron layered arrays (FeO x /NCLA) were simply constructed by pyrolyzing the composite precursor of NH 2 -MIL-101(Fe) (a metal organic framework, MOF) and glycylglycine. The experimental and calculated results reveal that the obtained catalysts exhibited multiple active components, synergistic heterojunction effect, high BET surface areas and conductivity, and consequently greatly improved intrinsic electrocatalytic activities and kinetics for oxygen reduction reaction (ORR) and oxygen evolution reaction (OER). As the results, the optimized catalyst FeO x /NCLA-1.2 exhibited positive half-wave potential of 0.846 V for ORR and only 218 mV of overpotential (at 10 mA/cm 2 ) for OER in alkaline electrolyte, which is superior than Pt/C (20 %) and RuO 2 , respectively. The Zn-air battery (ZAB) employing the FeO x /NCLA-1.2 as electrocatalyst obtained a peak power density of 104.3 mW·cm −2 , a specific capacity of 840 mAh·g Zn -1 and an energy density of 890 Wh·kg Zn -1 , which is also much higher than that for the noble metal based one. The catalyst is promised to be applied in water electrolysis, fuel cells and metal-air batteries.
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FOOD RESEARCH INTERNATIONAL
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2023
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IF 8.1
Hua Chen,Yousheng Shi,Lu Wang,Xiaoping Hu,Xue Lin
To investigate the changes in phenolics, flavonoids, and their bio-activities of wampee ( Clausena lansium (Lour.) Skeels) during digestion, the peel and pulp were subjected to simulated in vitro digestion, encompassing oral, gastric, small intestine, and large intestine digestion stages. The peel exhibited a total release of 91.93 mg GAE/g DW of phenolics and 61.86 mg RE/g DW of flavonoids, whereas the pulp displayed a release of 27.83 mg GAE/g DW of phenolics and 8.94 mg RE/g DW of flavonoids. Notably, the phenolics and flavonoids were mostly released during the oral digestion stage for peel, while they were mostly released during the small intestine digestion stage for pulp. The results of the targeted flavonoids analysis indicated that rutin and l-epicatechin were the two most widely released compounds in each digestion step. Moreover, myricetin has been identified as the best inhibitor against α -glucosidase, probably because it formed the most H-bonds, 8, with 6 catalytic residues, which was the highest number. Furthermore, the soluble substances released from the peel exhibited significantly higher antioxidant activities and inhibitory activity against α -glucosidase ( p < 0.05) compared to those from the pulp. Positive correlations were observed between the total phenolic content or total flavonoid content and the antioxidant activities (r > 0.73 (peel), > 0.61 (pulp)), as well as α -glucosidase inhibitory activity (r < − 0.48 (peel), < −0.64 (pulp)) of peel and pulp. In conclusion, these findings provide valuable insights into the digestive characteristics and health benefits of both wampee peel and pulp.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Qianqian Feng,Wei Yang,Zhiyun Peng,Guangcheng Wang
Oxalis corniculate L. ( O. corniculate ) was used to treat diabetes in Chinese folk as a popular tea drink. In this work, 31 compounds from O. corniculate were screened and identified as potential α-Glucosidase inhibitors (α-GIs). Among them, 6 compounds displayed stronger inhibitory activity than acarbose (IC 50 = 212.9 ± 5.98 μg/mL). Especially, the most effective compounds quercetin (Qu, IC 50 = 4.70 ± 0.40 μg/mL) and luteolin (Lu, IC 50 = 15.72 ± 0.75 μg/mL) inhibited α-Glu in competitive and mixed manners, respectively. Moreover, fluorescence quenching , circular dichroism (CD), and molecular docking study revealed that they can arouse the changes in the secondary structure and hydrophobic micro-environment of the enzyme mainly through a hydrophobic binding. Furthermore, it was observed that oral administration of Qu (20 mg/kg) can significantly reduce postprandial blood glucose (PBG) levels in mice vs. the control group. To sum up, the above research confirmed that O. corniculate could prevent and treat postprandial hyperglycemia as a good tea drink, and the plant was an excellent source to obtain natural α-GIs.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2023
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IF 9.9
Hongtong Hu,Yuyan Chang,Zhongjie Wang,Jiandong Cui,Shiru Jia,Yingjie Du
The combination of chemo- and biocatalysts to perform one-pot synthetic route has presented great challenges for decades. Herein, glutamate oxidase (GLOX) and trimanganese tetraoxide (Mn 3 O 4 ) nanocrystals were combined for the first time by one-step biomineralization to construct a mimic multi-enzyme system ( [email protected] 3 O 4 ) for chemoenzymatic synthesis of α‑ketoglutaric acid (α‑KG). Mn 3 O 4 not only served as a support for the enzyme immobilization, but also contributed its catalytic activity to co-operate with natural enzymes for the cascade reactions . The as-synthesized chemo-enzyme catalysts with directly contacted catalytic sites of the enzyme and inorganic catalyst maximizes the substrate channeling effffects for in situ rapid decomposition of the oxidative intermediate, H 2 O 2 , during the enzymatic oxidation of sodium glutamate, thus relieving the inhibition of H 2 O 2 accumulation for GLOX. Benefiting from the excellent stability and reusability of [email protected] 3 O 4 , a nearly 100% conversion (99.7%) of l -glutamate to α-KG was achieved, over 4.7 times higher than that of the free GLOX system (21.2%). This work provides a feasibility for constructing a high-performance chemo-enzyme catalyst for cascade catalysis, especially for those reactions with toxic intermediates.
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