Molecular Catalysis
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2024
·
IF 3.9
Guiyi Zhao,Weiguang Wang,Kaixuan Yang,Ting Su,Zhiguo Zhu,Hongying Lü
A deep eutectic solvent (DES) comprising p-toluenesulfonic acid (PTSA) and polyethylene glycol 200 (PEG200) was synthesized and applied to the Baeyer-Villiger (B-V) oxidation reaction of 2-adamantanone, which exhibited good catalytic effect and cyclic stability under relatively mild conditions. It was found that the hydrogen bond of DESs not only governed their physical properties, such as viscosity and electrical conductivity, but also influenced their chemical behavior, including the catalytic effect in B-V oxidation reaction. By adjusting the composition, the hydrogen bond properties can be optimized. This study aims to elucidate the intricate relationship between composition, hydrogen bond strength, and physicochemical properties of DESs, thereby establishing a preliminary theoretical foundation for a comprehensive understanding and construction of DESs systems. This provides a novel and promising environmentally friendly approach for the B-V oxidation reaction.
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Synthetic and Systems Biotechnology
·
2024
·
IF 4.4
Shanquan Liang,Zi He,Dan Liu,Shaoqing Yang,Qiaojuan Yan,Zhengqiang Jiang
2′-Fucosyllactose (2′-FL) is one of the important functional oligosaccharides in breast milk. So far, few attempts on biosynthesis of 2′-FL by the salvage pathway have been reported. Herein, the salvage pathway enzyme genes were introduced into the E. coli BL21star(DE3) for synthesis of 2′-FL. The 2′-FL titer increased from 1.56 to 2.13 g/L by deleting several endogenous genes on competitive pathways. The α-1,2-fucosyltransferase (WbgL) was selected, and improved the 2′-FL titer to 2.88 g/L. Additionally, the expression level of pathway enzyme genes was tuned through optimizing the plasmid copy number. Furthermore, the spatial distribution of WbgL was enhanced by fusing with the MinD C-tag. After optimizing the fermentation conditions, the 2′-FL titer reached to 7.13 g/L. The final strain produced 59.22 g/L of 2′-FL with 95% molar conversion rate of lactose and 92% molar conversion rate of fucose in a 5 L fermenter. These findings will contribute to construct a highly efficient microbial cell factory to produce 2′-FL or other HMOs.
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ACS Catalysis
·
2024
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IF 11.3
Yuexiang Li,Junliang Liu,Shuqi Li,Shaoqin Peng
Hydrogen production through electrochemical water splitting (EWS) presents a viable solution for addressing the fossil energy crisis. However, the commercial viability of this approach is impeded by the sluggish kinetics of the oxygen evolution reaction (OER). It is urgently needed to develop efficient, stable, and cost-effective OER electrocatalysts. Herein, we comprehensively design and investigate a phosphate ion and Fe3+ codecorating Ni(OH)2/NiOOH electrocatalyst (Pi-Fe:NiOH) for OER. This codecoration induces multiple synergistic effects, which include an increase in the interlayer water content for the internal OER, altering the OER mechanism, facilitating proton transport across the layers, and improving the stability of Pi-Fe:NiOH. Consequently, Pi-Fe:NiOH exhibits a high OER activity with overpotentials of 118 ± 1 and 222 ± 4 mV at current densities of 10 and 100 mA cm–2, respectively. More impressively, it maintains stable operation at a high current density of around 300 mA cm–2 for at least 500 h, much better than the Ni(OH)2/NiOOH electrocatalyst (NiOH) for less than 6 h at a current density below 200 mA cm–2. These findings offer insights for the design of anion–cation codoped hydroxide eletrocatalysts, paving a way for the development of efficient and stable OER electrocatalysts.
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MOLECULES
·
2024
·Vol. 29
·期 20
·4850
·
IF 4.2
Fei Liao,Jie He,Renjun Li,Yanchun Hu
Taking the endophytic fungus UJ3-2, isolated from Urtica fissa , as the experimental material, this study aimed to explore the composition of its metabolites and the underlying mechanisms by which it inhibits Staphylococcus aureus . Initially, the MIC, MBC, inhibitory curves, biofilm growth, and extracellular nucleic acids and proteins of S. aureus in response to the metabolites were measured. Secondly, PI staining and SEM were used to evaluate the impact of the metabolites on the integrity of the cell wall and overall morphology of S. aureus . Additionally, UPLC-MS was employed to analyze the composition of the secondary metabolites. The UJ3-2 strain was identified as Xylaria grammica based on ITS sequencing and designated as Xylaria grammica UJ3-2. Our results revealed that the metabolites of UJ3-2 exhibited excellent in vitro antibacterial activity against S. aureus , with both MIC and MBC values of 3.125 mg/mL. The inhibitory curve confirmed that 1 MIC of UJ3-2 metabolites could completely inhibit the growth of S. aureus within 24 h. With increasing concentrations of UJ3-2 metabolites, the growth of S. aureus biofilms was significantly suppressed, and obvious leakage of nucleic acids and proteins was observed. PI fluorescence staining indicated that various concentrations of UJ3-2 metabolites disrupted the integrity of the S. aureus cell membrane. SEM observation revealed that the treated S. aureus surfaces became rough, and the bacteria shrank and adhered to each other, showing a dose-dependent effect. UPLC-MS analysis suggested that the main components of the fermented metabolites were 6-oxocineole (17.92%), (S)-2-acetolactate (9.91%), 3-methyl-cis,cis-muconate (4.36%), and 8-oxogeranial (3.17%). This study demonstrates that the endophytic fungus UJ3-2 exhibits remarkable in vitro antibacterial effects against S. aureus , primarily by enhancing the permeability of the S. aureus cell membrane, causing the leakage of its intracellular contents, and altering the bacterial surface morphology to inhibit the pathogen. The endophytic fungus UJ3-2 has a good antibacterial effect on S. aureus , which gives it certain application prospects in the screening and industrial production of new and efficient natural antibacterial active substances.
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JOURNAL OF FOOD SCIENCE
·
2024
·Vol. 89
·期 12
·9271-9282
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IF 3.2
Jiayue Zhou,Dong Li,Yanqun Xu,Xiaochen Zhang,Lei Wang,Lin Zhou,Liang Zou,Zisheng Luo
( E )-2-Hexenal (E2H) is an important volatile organic compound (VOC) that can serve as a marker for fruit quality sensing and shelf-life evaluation. However, visual and portable sensors for E2H have not been reported mainly because of the difficulty in selective response to E2H while avoiding interference from other VOC, especially isomers. Herein, we developed a novel colorimetric sensor based on thiol-functionalized polydiacetylene assembly (PDA-SH/PDA) for the quantitative and selectivity of E2H. The mechanism was that E2H underwent a Michael addition reaction with sulfhydryl groups in PDA-SH/PDA. The Michael addition reaction destroyed the hydrogen bond and distorted the conjugated molecular system, resulting in colorimetric responses. Under optimal conditions, a good linear relationship was observed between E2H concentration and red colorimetric shift ( R 2 = 0.9679). The E2H sensor yields a high analytical sensitivity of 1.5203% and a limit of detection of 0.015 g L −1 . Besides, the PDA-SH/PDA sensor can be used to visually distinguish E2H and its isomer ( Z )-3-hexenal. The PDA-SH/PDA sensor yielded a statistically significant difference in red colorimetric shift ( p < 0.01) between the pairs of isomers in the ripeness of fruits. At last, the sensor was utilized for detecting E2H in grapes from different shelf lives. We found that the E2H concentration increased from 66.16 ± 1.54 to 67.56 ± 1.30 µg L −1 as storage time was prolonged. The results of this research not only demonstrated the feasibility of visual and on-site detection of E2H but also provided potential promise for portable fruit quality sensing and shelf-life evaluation.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Jianing Fu,Shaobo Li,Meizhen Xu,Ling Liu,Li Chen,Dequan Zhang
Soup is an important presence in diet, but its absorption and transport mechanism by the human body remains unclear. In this study, Caco-2 intact cell and monolayer cell models were constructed to simulate small intestine absorption on colloidal nanoparticles (CNPs) isolated from lamb soup. The intracellular localization of CNPs was viewed by laser confocal microscopy (LSCM). CNPs uptake and release pathways were explored by differences in CNPs concentrations in 5 endocytosis inhibitor models and 4 exocytosis inhibitor models. Results indicated that CNPs endocytosis by Caco-2 cells was restrained by Nystatin and Cytochalasin D, with exocytosis being inhibited by Nocodazole and Monensin. Therefore, the major absorption pathways for CNPs were caveolin-dependent endocytosis, macropinocytosis and phagocytosis. The major transport pathways were microtubule-vesicle-mediated protein transport to the membrane and transportation between the Golgi apparatus and membrane. This study may provide theoretical support for the transport mechanism of soup products in the small intestine.
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ANALYST
·
2025
·
IF 3.3
Yan Liu,Xue Kong,Yi Zhang,Xiumei Zhou,Zheng-Zhi Yin
A novel electrochemical microsensor is constructed on a traditional acupuncture needle (AN) and used to monitor a biomarker of the SARS-CoV-2-N protein. The reversible interaction of the borate bond between the cis-diol in this glycoprotein and the phenylboronic acid in 4-mercaptophenylboronic acid (4-MPBA) is effectively exerted. This interaction was applied to anchor the SARS-CoV-2-N protein onto 4-MPBA, which is covalently self-assemblied onto electrodeposited AuNPs by the S-Au bond. Meldola blue was then electropolymerized around the protein template. After the template was eluting, three-dimensional nanocavities complementary with the protein were generated within the polymelola blue (pMB) layer. Interestingly, nanocavities could play a channel role for the electron-transfer of outer [Fe(CN)6]3−/4−, and the signal of electrochemical probe could be hindered after recombining the SARS-CoV-2-N protein, which laid a platform for the detection of this biomarker. After optimizing the influencing factors, the prepared microsensor exhibits a linear range of 0.1 ~ 1000 ng mL−1 with a low detection limit of 0.01 ng mL−1 (S/N = 3). In particular, the sensing ability was dramatically affected by the thickness correlative factors for the polymer matrix. The suitable thickness is effective for the sensing signals, which corresponds to the behavior of surface-imprinted polymer. The microsensor showed comparatively high sensitivity and selectivity and practically detected the SARS-CoV-2-N protein in the serum sample, which is of scientific significance for the development of the electrochemical microsensor and acupuncture.
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MOLECULAR PHARMACEUTICS
·
2011
·
IF 5.4
Donghua Liu,Fengxi Liu,Zhihong Liu,Lili Wang,Na Zhang
Vascular endothelial growth factor receptors (VEGFRs) are overexpressed on the surface of a variety of tumor cells and on tumor neovasculature in situ, which are potential targets for “double targeting” (tumor- and vascular-targeting) tumor therapy. This study aimed to develop a VEGFR-mediated drug delivery system to target chemotherapeutic agents to VEGFR-overexpressed tumor cells and tumor neovasculature endothelial cells in vitro and in vivo. An antibody modified docetaxel (DTX)-loaded targeted nanostructured lipid carrier (tNLC) was designed and prepared with DSPE-PEG-NH2 as linker. The cellular cytotoxicity, cellular uptake, in vivo therapeutic effect and biodistribution of tNLC were investigated. The tNLC showed a particle size about 168 nm with encapsulation efficiency >95%, drug loading 5.55 ± 0.06% (w/w) and an average ligand coupling efficiency of 3.34 ± 2.63%. Cytotoxicity of tNLC against three human cell lines and one murine malignant melanoma was superior to that of Duopafei and nontargeted NLC (nNLC). The tNLC also showed better tolerance and antitumor efficacy in a murine model bearing B16 compared with Duopafei or nNLC. The studies on cellular uptake and biodistribution indicated that the better antitumor efficacy of tNLC was attributed to the increased accumulation of drug in both tumor and tumor vasculature. These findings suggested that tNLC designed to bind specifically to VEGFR-2 can be used to deliver DTX to the tumor vasculature and tumor and may inhibit tumor growth.
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TALANTA
·
2024
·
IF 5.6
Yingjiang Wang,Mingjing Li,Lingbo Qu,Lanlan Yu,Zhaohui Li
Laccase is well-known for its eco-friendly applications in environmental remediation and biotechnology, but its high cost and low stability have limited its practical use. Therefore, there is an urgent need to develop efficient laccase mimetics. In this study, a novel laccase-mimicking nanozyme (MBI-Cu) was successfully synthesized using 2-methylbenzimidazole (MBI) coordinated with Cu 2+ by mimicking the copper active site and electron transfer pathway of natural laccase. MBI-Cu nanozyme exhibited excellent catalytic activity and higher stability than laccase, and was utilized to oxidize a series of phenolic compounds. Environmental pollutant aminophenol isomers were found to display different color in solution when catalytically oxidized by MBI-Cu, which provided a simple and feasible method to identify them by the naked eye. Based on the distinct absorption spectra of the oxidized aminophenol isomers, a colorimetric method for quantitatively detecting o-AP, m-AP, and p-AP was established, with detection limits of 0.06 μM, 0.27 μM, and 0.18 μM, respectively. Furthermore, by integrating MBI-Cu-based cotton pad colorimetric strips with smartphone and utilizing color recognition software to identify and analyze the RGB values of the images, a portable colorimetric sensing platform was designed for rapid detection of aminophenol isomers without the need for any analytical instrument. This work provides an effective reference for the design of laccase nanozymes and holds significant potential for applications in the field of environmental pollutant monitoring.
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JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
·
2017
·Vol. 94
·期 12
·1481-1489
·
IF 1.421
Senan Ahmed M.,Zhang Sicheng,Qin Shuhao,Chen Zhuqi,Yin Guochuan
With the rapid depletion of fossil resources, the exploitation of biomass to partly replace fossil resources as the source of carbon in the chemical industry constitutes a promising alternative for the near future. This work introduces catalytic transformation of vegetable oil, i.e., methyl linoleate, to its conjugated esters by a simple Pd(OAc) 2 /Sc(OTf) 3 catalyst, which has extensive applications in industry. It was found that adding non-redox metal ions like Sc(III) to a simple Pd(OAc) 2 catalyst can effectively improve its isomerization activity in toluene/ t -BuOH solvent, whereas Pd(OAc) 2 alone is inactive. Preliminary mechanistic investigations together with previous studies suggested that the in situ-generated heterobimetallic Pd(II)/Sc(III) dimer serves as the key species for methyl linoleate isomerization, and the reaction proceeds by [1,3]-hydrogen shift mechanism involving a formal Pd(II)/Pd(IV) cycle.
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JOURNAL OF FOOD SCIENCE
·
2024
·
IF 3.2
Xiangjie Yao,Renkou Wan,Chunxiao Li,Gongjing Li,Bing Zhang,Zeyuan Deng,Hongyan Li
Bamboo shoot is a healthy food rich in dietary fiber (DF). However, its highly insoluble DF and fibrous texture limit its application in industrially processed foods. To achieve industrial processing of bamboo shoot, cellulase was used to improve the physical characteristics of bamboo shoot DF in this study. After enzymatic hydrolysis, the content of soluble DF (SDF) of bamboo shoot increased by 99.28% (from 5.53% to 11.02%) significantly ( p < 0.01). At the same time, the effect of enzymatic-modified bamboo SDF (EMBSDF) on streptozotocin-induced type 2 diabetes rats was explored. Results demonstrated that the high dose of EMBSDF (312.8 mg/kg) treated rats showed significant improvements in terms of glucose tolerance and insulin sensitivity ( p < 0.01) compared with the diabetes rats. Meantime, it was observed that the levels of glucagon-like peptide-1, adiponectin and interleukin-4 of high dose of EMBSDF compared with diabetes rats were increased ( p < 0.01) by 57.79%, 159.13%, and 6.17%, respectively. The tumor necrosis factor-α, C-reactive protein, and leptin levels were decreased ( p < 0.01) by 62.89%, 31.53%, and 7.84%, respectively. Furthermore, apparent kidney and pancreas histology improvements were found in high-dose and mid-dose EMBSDF-treated diabetes rats. These results indicated that the modified DF significantly improved diabetes.
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Matter
·
2024
·
IF 17.3
Xueao Jiang,Zhaoen Liu,Weijian Liu,Da Yu,Jun Zhang,Xiwen Wang,Yan Zhang,Shiguo Zhang
Ionic liquid (IL)-based gel electrolytes (ionogels) show great potential as quasi-solid-state electrolytes (QSSEs) for lithium-ion batteries (LIBs). However, conventional ionogels face challenges involving complex gelation processes, high gelator content (usually greater than 20 wt %), lower conductivity than neat ILs, and low Li + transference numbers. Here, we create novel lithium salt-containing supramolecular ionogels (SIGs) as efficient QSSEs for LIBs through a simple and environmentally friendly approach. By using a low-molecular-weight gelator, specifically 12-hydroxyoctadecanoic acid, lithium-containing IL electrolytes can be solidified at a gelator concentration of only 2 wt %. The resulting physical ionogels exhibit remarkable characteristics, including high ionic conductivity similar to neat ILs and improved electrochemical stability. In addition, these SIGs demonstrate a distinctive thermally reversible gel-to-sol transition, a quality not attainable in other QSSEs. This feature promotes better electrode/electrolyte contact, enabling excellent battery performance using LiFePO 4 , LiNi 0.8 Co 0.1 Mn 0.1 O 2 , and LiCoO 2 cathodes, along with Li metal and Li 4 Ti 5 O 12 anodes.
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