FOOD CHEMISTRY
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2019
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IF 5.399
Tiantian Min,Zhu Zhu,Xiaoli Sun,Zhipeng Yuan,Junwei Zha,Yongqiang Wen
In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method. A simple quaternization modification enabled the coating simultaneously to achieve excellent antifogging and antibacterial functions. The excellent antifogging property of the HACC/PVA composite coating was attributed to the strong water absorbency of quaternary ammonium chitosan and PVA. A nearly 98% transmittance ratio of coated glasses was achieved during antifogging test. In addition, the inhibition rate of the HACC/PVA composite coating kill against Escherichia coli ( E. coli ), Staphylococcus aureus ( S. aureus ) and Botrytis cinerea were up to ~99%. The antibacterial effect was demonstrated by each group of strawberries after storage for 1, 3, 5 days. The multifunctional coating has broad prospects in the application of fruit and vegetable packaging.
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ACS Biomaterials Science & Engineering
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2019
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IF 4.511
Ruitao Zou,Suyan Shan,Libin Huang,Zhi Chen,Tom Lawson,Mimi Lin,Lu Yan,Yong Liu
The noninvasive and real-time detection of glucose sugar from tears is promising for the early diagnosis and treatment of chronic diseases such as diabetes. However, its realization is a big challenge. A suitable biosensor electrode that can closely fit the eye and be electrochemically sensitive is still unrealized. In this work, nitrogen-doped graphene (N-G) was used as an ophthalmic electrode in a high-performance intraocular biosensor. The use of N-G has been reported elsewhere before as it is highly electroactive and so has a particular use in biosensors. We hereby present a novel procedure for making carboxylated chitosan-functionalized nitrogen-containing graphene (GC–COOH) by using a one-step ball-milling process. This process does not use toxic chemicals, flammable gases, or a high temperature. It is thus particularly easy to perform. The fabricated nanomaterial had a high electroactivity and was easily assembled as a glucose biosensor by the immobilization of glucose oxidase. The thus constructed biosensor has a high sensitivity at 9.7 μA mM–1 cm–2, a broad linear range at 12 mM, and a good detection limit of 9.5 μM. It was able to maintain this activity after a month of storage. We also report the intraocular use of this constructed biosensor. The as-prepared GC–COOH was found to be highly biocompatible to ophthalmologic cells such as corneal epithelial and retinal pigment epithelium cells. No change in the intraocular pressure or the corneal structure was measured in a New Zealand white rabbit model. The as-assembled sensor was worn by the animals for more than 24 h without undue impact. This result confirmed the biosensor’s potential for intraocular application in the clinic. Its assembly into a useful sensor shown here has great potential to provide real-time monitoring of glucose levels in tear fluids of patients with high sugar levels.
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FOOD CHEMISTRY
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2019
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IF 5.399
Dan-Dan Li,Yang Tao,Ya-Ning Shi,Yong-Bin Han,Na Yang,Xue-Ming Xu
This study explored the impact of re-acetylation on induced electric field (IEF)-assisted hydrolysis of chitosan. Chitosan with different degree of deacetylation (DD) exhibited different charge content but similar electrical conductivity and output voltage of the electrolyte system, thus theoretically resulting in equivalent electro-processing efficiency. However, molecular and rheological measurements suggested that the hydrolysis rate decreased with an increase of DD. Re-acetylation had no significant effect on the molecular weight and viscosity of chitosan but damaged its granular and crystal structure, making chitosan chains more susceptible to the acid. The IEF-assisted hydrolysis of dissolved chitosan confirmed that the structural compactness of chitosan played a more important role on the hydrolysis than the charge content. This study extended current knowledge regarding the effect of charge content and structural compactness on IEF-assisted hydrolysis process, which contributed to the applications of IEF in biopolymer modification.
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FOOD HYDROCOLLOIDS
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2022
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IF 11.504
Ya-Ru Wang,Qin Yang,Ya-Nan Du,Han-Qing Chen
Food protein fibrils display superior mechanical and interfacial properties compared to the native protein. During processing, protein fibrils are stored for a long time and undergo pH shifts, which accelerate their degradation. In this study, the effects of chitosan on the morphology, structural and rheological properties of ovalbumin (OVA) fibrils after storage at pH higher than isoelectric point were studied. The results showed that OVA fibrils prepared at pH 2.0 remained stable after 7 days of storage. However, after storage at pH 8.0 for 7 days, the turbidity of OVA fibrils increased, the contour length and ThT fluorescence intensity decreased. Furthermore, OVA fibrils became stable again by interacting with chitosan via electrostatic interaction, which was confirmed by zeta potential . In addition, based on the results of Fourier transform infrared spectroscopy , sodium dodecyl sulfide polyacrylamide gel electrophoresis , and fluorescence spectra, it could be concluded that chitosan could interact with OVA fibrils at pH 8.0 during storage. Transmission electron microscope images indicated that the structure of OVA fibrils was maintained by the addition of positively charged chitosan. Finally, the viscosity and storage modulus of OVA fibrils after storage at pH 8.0 increased with the addition of chitosan. These findings implied that OVA fibrils were degraded when stored at pH above isoelectric point, but this degradation could be ameliorated by the addition of chitosan.
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CARBOHYDRATE POLYMERS
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2022
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IF 10.723
Yue Zhu,Di Qin,Jinfeng Liu,Guangsheng Wu,Haonan Wang,Feifei Wu,Yixuan Liu,Ya Liu,Xiaojie Cheng,Xiguang Chen
Hydrogel, as three-dimensional (3D) cell culture scaffold, is an effective strategy for tissue and organ regeneration due to their good biocompatibility, biodegradability and resemblance to body microenvironments in vivo. However, the inherent weak mechanical properties and strong shrinkage of hydrogels during cell culture hinder its application in clinical. In this study, a two-component thermo/photo dual-sensitive hydrogel (M/C) was prepared from methacrylated hydroxybutyl chitosan (MHBC) and chitin whisker (CHW) via physical and chemical cross-linking methods. M/C hydrogel showed a special internal structure with lamellar arrangement. The rheological properties of the hydrogels could be regulated with the change of M/C ratio. It is worth emphasizing that the mechanical properties, shrinkage resistance and cellular capacitances of the M/C hydrogel were improved with the addition of CHW. Moreover, the M/C hydrogel not only exhibited excellent degradability and antibacterial properties , but also significantly promoted the adhesion and proliferation of MC3T3-E1 cells in vitro. Therefore, the M/C hydrogel showed a wide application potential in tissue regeneration as a 3D cell culture scaffold.
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Marine Drugs
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2022
·Vol. 20
·期 11
·688
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IF 6.085
Jingmin Cui,Yan Sun,Linqing Wang,Qin Miao,Wenqiang Tan,Zhanyong Guo
We successfully prepared a series ofl-arginine Schiff bases acylated chitosan derivatives, aiming to improve the antioxidant activity and antimicrobial activity of chitosan by introducing a furan ring, pyridine ring, andl-arginine structure. The accuracy of the structures of ten compounds was characterized by FT-IR and1H NMR. In terms of DPPH radical scavenging activity, except for compound CR3PCA, the scavenging rate of other compounds was higher than chitosan, especially CRCF and CRBF had strong scavenging abilities. At the same time, in the superoxide-radical scavenging activity assay, CRCF, CRBF, CR3PCA, CR2C3PCA, and CR2B3PCA were comparable to positive control at 1.60 mg/mL. Simultaneously, CRFF, CRCF, and CRBF had a certain inhibitory effect onBotrytis cinerea. Furthermore, the inhibitory effect of CRFF, CRCF, and CR3PCA onStaphylococcus aureuswas very well, close to the positive control at 1.00 mg/mL. CRCF and CR2B3PCA showed better inhibitory effects onEscherichia colithan other compounds. The MTT assay was used to determine the cytotoxicity of the chitosan derivatives, which proved their safety to fibroblast cells. In summary, the study indicated that some of these compounds have the potential for further development and utilization in the preparation of antioxidants and antimicrobial agents.Keywords:chitosan;l-arginine Schiff bases;heterocyclic compounds
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Biomolecules
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2022
·Vol. 12
·期 11
·1601
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IF 6.064
Weiyan Quan,Puwang Li,Jinsong Wei,Yuwei Jiang,Yingye Liang,Weilin Zhang,Qizhou Chen,Kefeng Wu,Hui Luo,Qianqian Ouyang
Creation of bio-multifunctional wound dressings with potent hemostatic, antibacterial, anti-inflammatory, and angiogenesis features for bolstering the healing of full-thickness wounds is sought after for clinical applications. We created bio-multifunctional composite sponges by coupling alginate and chitosan withSargassum pallidumpolysaccharides through electrostatic interactions, calcium ion (Ca2+) crosslinking, and lyophilization. Alginate/chitosan (AC) sponges with different concentrations ofSargassum pallidumpolysaccharides were obtained and termed AC, ACS—1%, ACS—2.5%, and ACS—5%. ACS—1% and ACS—2.5% sponges exhibited uniform porosity, high water vapor transmission rate, high water absorption, as well as good hemostatic and antibacterial abilities. ACS—2.5% sponges facilitated wound closure and promoted angiogenesis and re-epithelialization in the dermis. These data suggest that ACS sponges containing a certain amount ofSargassum pallidumpolysaccharides could be employed for treatment of full-thickness skin wounds.Keywords:Sargassum pallidumpolysaccharides;bio-multifunctional;composite sponges;wound healing;alginate;chitosan
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DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
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2019
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IF 2.367
Jingwen Luo,Jiang Sun,Xiaoting Luo,Yinghui Wei,Hangsheng Zheng,Chaofeng Mu,Wenjie Yao
Objective: This article aims to design low molecular weight chitosan (LMWC)-based conjugates of Rhein (RH) by means of an amino acid linker (Alanine) for improved solubility and enhanced bioavailability.Significance: Rhein is a potential candidate for the therapy of kidney disease. However, the poor solubility, inadequate bioavailability, and lack of proper formulation restrict its clinical applicability. LMWC-drug conjugates offer the potential to improve the water-solubility of RH, increase its oral absorption, and thereby enhance its bioavailability.Methods: The conjugates were synthesized via a carbodiimide reaction and confirmed using UV-vis, FTIR, and 1H-NMR spectroscopy. The water-solubility and in vitro release properties were evaluated. Free RH and RH-LMWC conjugates were administered at an equivalent oral gavage dose of RH at 35 mg/kg for pharmacokinetic studies in Sprague Dawley rats.Results: The conjugates with RH content of 9.65% were successfully synthesized and featured a satisfactory water-solubility of 9.73 mg/mL, which exhibited a sustained release pattern over 72 h, and the enzymes present may promote the degradation of the conjugate to increase the release of Rhein. Oral administration of RH-LMWC conjugates to rats led to seven-folds and 3.1-folds increase in the T1/2 and AUC0–∞, respectively, as compared to RH suspension.Conclusion: The present work demonstrated that the RH-LMWC conjugates exhibited sustained release properties with outstanding oral bioavailability enhancements compared to administration of RH itself. Potentially, RH-LMWC conjugates may serve as a promising lead for developing a new platform for RH oral delivery.
研究领域:
药剂学
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药物化学
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生物医学工程
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TENSIDE SURFACTANTS DETERGENTS
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2023
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IF 1
Bangchang Wei,Ya Xu,Shengnan Kong,Zheng Ji,Yansong Zhang,Huchuan Wang,Chuanrun Li
As a natural, non-toxic adsorbent, chitosan is well suited for the removal of heavy metals and antibiotics. In this study, chitosan was modified with polypropylene glycol and itaconic anhydride to improve the mechanical properties of chitosan, and the polypropylene glycol-itaconic anhydride-chitosan adsorbent was successfully prepared. The adsorbent was characterised and analysed by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis and X-ray photoelectron spectroscopy (XPS). The effects of the different conditions on the adsorption of Pb 2+ and cephalexin were investigated. The Langmuir adsorption isotherm model showed that the maximum adsorption capacities of Pb 2+ and cephalexin could reach 431.73 mg g −1 and 153.72 mg g −1 , respectively. The possible adsorption mechanism of Pb 2+ and cephalexin was discussed.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Dongxing Dong,Xiaoli Lv,Qiushi Jiang,Jingjing Zhang,Zhengyi Gu,Weimin Yu,Zhaolian Han,Ning Wang,Wenli Hou,Zhiqiang Cheng
In this study, a multifunctional nanofiber dressing that can promote antibacterial, analgesic and healing was prepared by electrospinning technology. Hydrophobic polycaprolactone (PCL)/chitosan (CS)/lidocaine hydrochloride (LID) and epidermal growth factor (EGF) were used as scaffold materials and dissolved in trifluoroacetic acid to prepare spinning solution. The morphology of PCEL dressing was observed by scanning electron microscopy. The fiber structure was dense and the average diameter was 297.0 nm. The water absorption capacity test and water contact angle measurement showed that the fiber had good water absorption and hydrophilicity (1302 %, 139.258°). Drug release was 84 % within 60 h. In the results of antibacterial experiment, the dressing showed certain antibacterial properties . The results of cell experiments show that the dressing can promote cell proliferation . In addition, coagulation experiments showed that the dressing could quickly coagulate the blood within 4 min. In addition, PCEL dressing promoted collagen deposition and vascularization through animal models of pressure sores. Therefore, multifunctional dressing can be used as an ideal auxiliary means for the treatment of pressure sores, and it is a promising alternative to chronic wound healing.
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Scientific Reports
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2023
·Vol. 13
·期 1
·1-18
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IF 4.6
Zhang Meng,Liu Yunqing,Yin Zhizhen,Feng Dan,Lv Hui
The magnetic chitosan/sludge biochar composite adsorbent was prepared using chitosan, Fe 3 O 4 , and sludge biochar as raw materials. The composite adsorbent was able to achieve rapid solid–liquid separation under an applied magnetic field. The morphology and microstructure of the composite adsorbent were characterized by FTIR, XRD, SEM, VSM, and BET analysis. The adsorption performance of the composite adsorbent on Cu 2+ was investigated through static adsorption experiments, and the effects of adsorbent dosage, initial concentration of Cu 2+ , initial pH of the solution, and adsorption temperature on the adsorption efficiency of Cu 2+ were discussed. The results showed that chitosan and Fe 3 O 4 were successfully loaded on sludge biochar. When the initial concentration of Cu 2+ was 30 mg/L, the dosage of the magnetic chitosan/sludge biochar composite material was 0.05 g, the adsorption time was 180 min, pH was 5, and the temperature was room temperature, the maximum removal rate of Cu 2+ reached 99.77%, and the maximum adsorption capacity was 55.16 mg/g. The adsorption kinetics and adsorption isotherm data fitted well with the pseudo-second-order kinetic model and Langmuir adsorption isotherm model, indicating that the adsorption process was chemisorption with monolayer coverage.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Haoming Gu,Xijun Wang,Shuibo Zheng,Haisong Qi
Chitosan-based films suffer from issues such as poor plasticity and inadequate barrier properties. Herein, high toughness chitosan-based films were fabricated by chemical crosslinking between chitosan with acetoacetylated poly(vinyl alcohol) via the Mannich multicomponent reaction. The structure and properties of the resulting chitosan-based composite films were comprehensively investigated by methods such as FT-IR analysis, acid treatment, tensile testing and so on. The results demonstrated that the constructed network structure significantly enhanced the mechanical performance of the films with a tensile strength of 74.4 MPa and an elongation at break of 102.7%. Especially, the toughness of chitosan-based composite films reach up to 67.6 MJ/m 3 , which is much higher than those reported in the literature. Meanwhile, these films exhibited low oxygen and water vapor permeability coefficients of 8.6 × 10 −6 and 4.5 × 10 −6 mol·mm·m −2 ·d −1 ·kPa −1 , respectively. Application in strawberry preservation demonstrated that the films effectively regulated the micro-environment, extending shelf life by 6 days. This study provides a sustainable and efficient strategy for designing high-performance bio-based packaging films for strawberry preservation.
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