TALANTA
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2026
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IF 6.7
Dan Huang,Ling Li,Renjie Li,Wenxi Wang,Wenli Chen,Xiandeng Hou,Li Wu
Tetracycline hydrochloride (TCH) is a widely used antibiotic in animal husbandry and aquaculture practices, and its residual accumulation in food commodities constitutes a persistent public health concern. Therefore, sensitive, time-saving, and reliable analytical strategies for TCH are critical for guaranteeing food safety. Surface-enhanced Raman spectroscopy (SERS) has become an ideal analytical modality, attributed to its ultrahigh sensitivity, operational simplicity, and inherent molecular fingerprinting capability. The integration of plasmonic metal materials (PMPs) with the functional material provides a rational design strategy for advanced SERS substrates. A case in point is that of metal-organic frameworks (MOFs). However, the construction of MOF-PMPs composites is hindered by complex fabrication protocols, prolonged preparation time and high cost. Electrochemical deposition, characterized by rapid processing, facile parameter tuning, and environmental compatibility, presents a promising alternative for SERS substrate construction. In this work, we present a novel Ag/Zn-BTC@NF composite SERS substrate synthesized via two-step electrodeposition. The as-fabricated material delivers prominent SERS sensitivity. When R6G is used as the specific molecule, LOD (detection limit) can reach 10 −9 M, and the enhancement factor is 6.6 × 10 6 . Furthermore, the substrate achieved an LOD of 10 −8 M for TCH and enabled its ingenious detection in milk and honey samples, with recoveries of 87.4 − 112.5%. The proposed methodology establishes an efficient and eco-friendly synthetic strategy for MOF-PMPs composites, thereby broadening the scope of SERS technology in food safety applications.
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Food Bioscience
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2026
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IF 6.2
Cuicui Duan,Hui Liu,Siyang Sui,Hongru Wang,Yinfei Xing,Yixue Sun,Lifan Liu,Fumin Ma,Xiaolei Li,Dan Li
As a common probiotic, Lactobacillus plantarum has a unique probiotic potential in improving metabolic diseases, but its protective effects and specific mechanism on type 2 diabetes (T2D) are still unclear. This study systematically evaluated the alleviating effect of L. plantarum LH36 on the T2D mice model induced by high-fat diet (HFD) combined with streptozotocin (STZ) through blood physiological indexes, histopathological changes, intestinal flora and non-targeted metabolomics analysis. The results showed that after oral administration of L. plantarum LH36, the loss of body weight, blood glucose regulation capacity and insulin resistance of T2D mice were significantly improved. The histopathological damages to the colon and liver were also effectively alleviated. In addition, the intestinal flora sequencing results showed that L. plantarum LH36 treatment reversed the structural disorder of intestinal flora induced by HFD + STZ, improved the richness, diversity and evenness of the intestinal flora, including Chao1, Shannon, and Simpson indices. Specifically, Bacteroidetes was increased but Firmicutes was decreased at the phylum level. The disorders of Lactobacillus and Butyrobacter were restored at the genus level. In addition, non-targeted metabolomics results further confirmed that L. plantarum LH36 treatment could significantly restore the metabolic disorder in T2D mice and regulate multiple metabolites with diverse functions, including various long-chain acylcarnitine, diacylglycerols and pentadecanic acid, etc. These results verified that L . plantarum LH36 treatment exerted hypoglycemic effects by improving insulin resistance, reducing blood lipids, alleviating pathological changes in the colon and liver, as well as adjusting the intestinal flora disorders and regulating the host's metabolic profiles. These results offer crucial scientific support for developing L. plantarum as a promising adjunct therapeutic approach for T2D.
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INDUSTRIAL CROPS AND PRODUCTS
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2026
·
IF 6.4
Xiangyang Pan,Rui Xie,Shaoxiong Zhu,Deshui Yu,Yang Yang,Guang Yang,Bo Huang
Cationic nanocarriers retain 80% pesticide on leaves versus 42% in neutral formulations via electrostatic adhesion. • EB@PCBs maintain 80% concentration after 5 h UV exposure compared to 40% for free EB. • 78.90% drug loading efficiency overcomes water dispersibility and retention limitations of conventional pesticide formulations. 78.90% drug loading efficiency overcomes water dispersibility and retention limits of conventional formulations. • EB@PCBs persist 120 days in field versus 30 days for conventional EB, reducing application frequency. Pine wilt disease, caused by the pine wood nematode ( Bursaphelenchus xylophilus , PWN), represents a significant threat to pine forests ( Pinus koraiensis Siebold & Zucc and Pinus massoniana Lamb) across Asia, particularly in China. While Emamectin benzoate (EB) is commonly employed for pest control, conventional formulations suffer from low efficacy due to photodegradation, poor water dispersibility, and inadequate leaf surface retention—with only 0.1% of applied pesticide reaching the target. This study developed a series of polymer nanocarriers with tunable surface charge properties via reversible addition-fragmentation chain transfer (RAFT) polymerization-induced self-assembly (PISA). Three nanocarrier variants were fabricated: neutral PTOG-PBMA (POBs), anionic PAA-PBMA (PABs), and cationic PDMA-PBMA (PCBs). All nanocarriers achieved drug loading efficiencies exceeding 65%, with PCBs reaching 78.90%. Critical performance metrics demonstrated the superiority of cationic carriers: following 10 mm simulated rainfall, EB@PCBs retained 80% of applied pesticide on pine needles versus only 42% for neutral and 57% for anionic formulations. Under UV irradiation (365 nm), EB@PCBs maintained 80% of its initial concentration after 5 h, compared to 40% for free EB. In field trials on P. massoniana , EB@PCBs persisted at 0.4% of initial concentration 120 days post-application, whereas conventional EB was depleted within 30 days. These results demonstrate that charge-controlled polymer nanocarriers represent an effective strategy for improving pesticide utilization efficiency and environmental persistence, offering a practical solution for sustainable forest disease management. Download: Download high-res image (185KB) Download: Download full-size image
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Nature Communications
·
2026
·
IF 18.1
Peng Haibao,Zeng Youming,Huang Yang,Lin Chenyu,Lei Zeyu,Sun Peixin,Yang Jiahui,Sheng Shaoqi,Huang Yuxin,Li Xiaohui,Zhao Jiaxu,Zeng Rui,Li Cheng,Yang Wanqi,Di Xiangjie,He Sisi,Wang Yongsheng,Yang Hanting,Yang Wuli,Chi Yudan
The central nervous system is a critical reservoir for cancer metastasis, underscoring the urgent need for effective strategies for targeted drug delivery that minimize systemic side effects. Here, we present a zwitterionic polycarboxybetaine (PCB) designed to achieve selective accumulation within the tumor vasculature of brain metastases, thereby facilitating the rapid release of therapeutics without relying on external stimuli in preclinical models. PCB selectively targets the betaine-γ-aminobutyric acid transporter 1 (BGT1) at the blood-brain-tumor barrier (BBTB), enabling localized enrichment of nanoparticles within the tumor vasculature. The intrinsic mechanical flexibility of PCB activates the Piezo1 ion channel in endothelial cells, resulting in calcium ion influx and enhanced calpain activity, which together facilitate the degradation of VE-cadherin. The burst-release mechanism improves vascular permeability and compromises the integrity of the tumor vascular barrier, allowing for the swift release of encapsulated therapeutics and promoting extensive drug distribution within the tumor microenvironment. Notably, PCB encapsulating either osimertinib or cisplatin demonstrates therapeutic efficacy, intratumoral targeting, and favorable biological safety in disease models. These findings indicate that this zwitterion-functionalized platform effectively surmounts the challenges posed by the BBTB, supporting improved drug delivery and therapeutic efficacy in brain metastases.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2026
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IF 5.2
Xiaoxi Gao,Dehai Hou,Tianyu Ma,Sizhuo Liu,Ruiping Wang,Yang Ping,Jin Su
A synergistic nano-strategy co-delivering sorafenib and berberine to overcome resistance and reduce toxicity. • Optimized nanostructured lipid carriers with high drug loading, sustained release, and good stability were successfully prepared. • Pharmacokinetics showed significantly enhanced oral bioavailability (4.59-fold and 2.28-fold for SORB and BBR). • Superior antitumor efficacy and biosafety were systematically verified both in vitro and in vivo. Sorafenib (SORB) is a first-line drug for advanced hepatocellular carcinoma, but its clinical use is limited by poor water solubility, low oral bioavailability, and drug resistance. Berberine (BBR) has been shown to reverse multidrug resistance and enhance drug sensitivity. Here, we designed a nanostructured lipid carrier co-loaded with SORB and BBR (SBER NLC). The optimized formulation had a particle size of 123.17 ± 3.28 nm, PDI of 0.211 ± 0.012, and zeta potential of −18.30 ± 0.95 mV. Encapsulation efficiencies were 78.77 ± 0.93% (SORB) and 79.58 ± 1.04% (BBR), with drug loadings of 1.75 ± 0.06% and 1.28 ± 0.13%, respectively. SBER NLC showed stable storage properties and sustained release in simulated gastrointestinal fluids. Pharmacokinetic studies demonstrated significantly enhanced oral bioavailability of both drugs compared with free drug suspension. In vitro, SBER NLC exhibited stronger cytotoxicity and migration inhibition against HepG2 cells, while the blank carrier was nontoxic. In a HepG2 xenograft mouse model, SBER NLC achieved the strongest tumor suppression without causing body weight loss or major organ damage, and induced extensive nuclear pyknosis and necrosis in tumor tissue. This study provides an efficient nano-delivery strategy for combined SORB and BBR in hepatocellular carcinoma treatment. Download: Download high-res image (226KB) Download: Download full-size image
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PHYTOTHERAPY RESEARCH
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2026
·
IF 8.1
Jing Li,Yang-Chi-Dung Lin,Hsi-Yuan Huang,Jihang Chen,Huali Zuo,Yun Tang,Hsien-Da Huang
Obesity is a major risk factor for type 2 diabetes. Some traditional Chinese medicine (TCM) compounds can improve obesity by increasing energy consumption. This study aimed to identify and verify TCM compounds that promote adipose thermogenesis to alleviate obesity via transcriptomic analysis, molecular docking, network pharmacology, and Connectivity Map (CMap) analysis. Thirty-six TCMs related to adipose tissue thermogenesis were first collected to generate transcriptomic data. Then a ranking method based on integrated transcriptomic data was used to select emodin from the TCM Da Huang for network pharmacology to explore its potential mechanisms and further experimental validation. CMap analysis of thermogenesis signature genes from ProFAT and GEO datasets identified triptolide as another compound for improving obesity through enhanced thermogenesis. In vitro experiments with 3T3-L1 cells and in vivo experiments with zebrafish were conducted. The results showed that both emodin and triptolide could upregulate the NAD + /NADH ratio, increase AMPK and SIRT1 expression, suppress lipid accumulation, and promote lipolysis, as verified by both in vitro and in vivo experiments. They also upregulated thermogenesis-related genes (e.g., UCP1, PGC1α, and PRDM16), lipolysis-related genes (e.g., PKA, ATGL, and HSL), mitochondrial biogenesis-related genes (e.g., NRF1, NRF2, and TFAM), and β-oxidation-related genes (e.g., CPT1α and CPT1β). Meanwhile, they downregulated lipogenesis-related genes such as FASN, SREBP1, and ACC. These results provide a basis for understanding the effects of emodin and triptolide on obesity. The findings highlight the effectiveness of combined screening approaches in exploring compounds with beneficial metabolic effects.
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JOURNAL OF CONTROLLED RELEASE
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2026
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IF 12.4
Renfang-Zhu,Ying Xu,Mingyu Nie,Huiling Li,Ye Yuan,Zhixiang Cui,Hezhi Wang,Qiyao Zhai,Xianhong He,Ying Mao,Yidan Wei,Jian Guan,Xin Zhang,Shirui Mao
Clinically, no effective preventive strategies currently exist to inhibit the progression of early dry age-related macular degeneration (dAMD). Moreover, current retinal therapies rely on intravitreal injections, which carry inherent procedural risks. Inspired by natural melanin and viral structures, we developed virus-biomimetic melanin nanoparticles (VMNPs) surface-modified with ergothioneine (ET), featuring virus-like spike structures and a neutral zwitterionic shell. This study aimed to evaluate whether virus-inspired engineered nanoparticles could enhance retinal delivery and improve the therapeutic potential of melanin-based nanodrugs for dAMD. The VMNPs facilitated cellular uptake through both their virus-mimetic architecture and OCTN1-mediated active transport. They also exhibited potent antioxidant activity, significantly reducing intracellular and mitochondrial reactive oxygen species (ROS) in oxidative stress models of ARPE-19 cells. Transcriptomic analyses revealed that VMNPs activated intrinsic cytoprotective pathways by upregulating HMOX1 and SLC7A11 , while downregulating inflammation-associated genes. Importantly, VMNPs promoted transcytosis and, following topical administration, demonstrated improved retention and deep penetration within the rabbit eye, effectively reaching the retinal pigment epithelium (RPE) and deeper retinal layers. Notably, twice-daily topical administration of VMNPs markedly inhibited retinal degeneration in rabbits, achieving therapeutic efficacy comparable to a single intravitreal injection of pegcetacoplan. Collectively, these findings establish a promising paradigm for the non-invasive clinical treatment of dAMD.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2026
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IF 5.4
Jie Ding,Jing Zhang,Boxia Liu,Yuyu Wang,Dongxu Han,Yeqiong Huang,Chuhan Xing,Zhi Song
MnFe-O activates PMS to remove 99.34% phenol within 30 min. • Oxygen vacancies regulate Mn-Fe dual-site electronic coupling. • MnFe-O shows higher Fe 2+ and Mn 3+ contents for rapid redox cycling. • O 2 •− / 1 O 2 -coupled pathways dominate phenol oxidation in MnFe-O/PMS. • MnFe-O/PMS maintains high activity in diverse real water matrices. Peroxymonosulfate (PMS) activation technology has significant application potential in the removal of phenolic pollutants, but its catalytic performance is still limited by problems such as insufficient regulation of the electronic structure of active sites and unclear interfacial reaction pathways. This study used MnFe Prussian blue analogue (MnFe-PBA) as a precursor to construct a series of MnFe-X (X = O, S, Se) catalysts through thermal conversion and oxygen vacancy-related defect engineering, and systematically compared the effects of different vacancy types on the PMS-activated degradation performance of phenol. The results showed that the MnFe-O sample, which was jointly regulated by oxygen vacancy-related defects and structural reconstruction, exhibited the best catalytic activity, achieving 99.34% phenol removal within 30 min, with an apparent rate constant as high as 0.20 min −1 , which was significantly better than the MnFe-PBA and S/Se vacancy-regulated samples. XPS analysis showed that the increased proportions of Fe 2+ (77.25%) and Mn 3+ (70.00%) in MnFe-O created a more favorable local electronic environment for electron coupling and redox cycles. EPR and quenching results indicate that •OH, SO 4 •− , O 2 •− and 1 O 2 are generated simultaneously in the system, and an oxidation pathway dominated by O 2 •− / 1 O 2 coupling is ultimately formed. This work reveals the key role of oxygen vacancy-related defects and structural reconstruction in jointly regulating the activation of PMS by Mn-Fe dual sites, providing a new mechanistic basis for defect engineering-driven bimetallic catalyst design and efficient purification of phenolic wastewater. Download: Download high-res image (230KB) Download: Download full-size image
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2026
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IF 9.1
Bin Wen,Fan Chen,Yi Ran,Dongyun Que,Zhihao Zhang,Youwei Zeng,Yuyuan Yao
Molybdenum disulfide (MoS₂) is a promising inorganic co-catalyst for Fenton processes due to its structural durability and avoidance of secondary organic pollution. However, the conventional 2H-phase MoS₂ suffers from intrinsically low conductivity, severely limiting Fe 3+ reduction in the Fenton reaction. Herein, L-cysteine served as a multifunctional precursor during the preparation of MoS₂, promoting a slow-release sulfur supply, carbon‑nitrogen co-doping, and the prevention of restacking. The carbon and nitrogen co-doped molybdenum disulfide (CN-MoS 2 ) architecture was fabricated via a single-step hydrothermal reaction mediated by sodium molybdate, thiourea, and L-cysteine. Remarkably, benefiting from the introduction of L-cysteine, the resulting CN-MoS 2 exhibited a high 1 T-phase content (68%), abundant sulfur vacancies, and a substantially reduced particle size. CN-MoS 2 accomplished near-complete elimination of carbamazepine (CBZ) within merely 3 min, yielding a prominent apparent rate constant of 0.7111 min −1 , which is a 19-fold kinetic acceleration over thiourea-derived MoS 2 . CN-MoS₂ also exhibited high TOC removal, broad-spectrum degradation, and robust CBZ removal under various water conditions. Additionally, electrochemical analyses further verified that CN-MoS₂ reduced charge-transfer resistance and enriched electron density, thereby facilitating rapid electron shuttling to Fe 3+ . Concurrently, quenching experiments and electron paramagnetic resonance (EPR) diagnostics unambiguously validated SO 4 •- , 1 O 2 and O 2 •- as the driving reactive forces. Moreover, a continuous-flow device incorporating a CN-MoS 2 -based membrane exhibited outstanding performance in wastewater purification. This work offers a new strategy for designing high-activity co-catalysts for advanced oxidation processes.
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ANALYTICA CHIMICA ACTA
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2026
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IF 6.1
Nanke Ma,Anqi Wei,Huiling Peng,Lixia Xie,Yuan Jin,Zhengzhi Yin,Guangli Li
Background Chirality plays a critical role in drug efficacy and environmental safety. The antibacterial activity of ofloxacin resides almost exclusively in its S-enantiomer (levofloxacin, LEV), while the R-enantiomer contributes little to therapeutic effects but may pose unnecessary risks. Rapid and reliable methods for enantioselective determination of ofloxacin enantiomers are therefore essential for pharmaceutical quality control, food safety monitoring, and environmental analysis. However, existing electrochemical sensors often suffer from limited enantioselectivity and insufficient sensitivity. To address this challenge, a novel enantioselective electrochemical platform is developed. Results A β-cyclodextrin/cerium-doped copper metal-organic framework/carboxylated multi-walled carbon nanotube composite (β-CD/Ce-doped Cu-MOF/MWCNTs-COOH) was fabricated on a glassy carbon electrode. The sensor achieves discrimination of ofloxacin enantiomers with an I L /I D ratio of 3.39. DFT calculations at pH 4.0 reveal that β-CD binds LEV with higher adsorption energy (−63.8 kJ/mol) than DEX (−58.3 kJ/mol), confirming the superior response for LEV originates from stereospecific inclusion. Quantitative analysis of LEV shows two linear ranges (0.03–1 μM and 1–10 μM) with a low detection limit of 0.01 μM. The sensor also enables accurate determination of LEV percentage in enantiomer mixtures across 0–100%. Excellent selectivity against common interferents, good reproducibility (RSD = 0.98%), repeatability (RSD = 1.26%), and long-term stability (95.2% retention after 21 days) are demonstrated. Practical applicability is validated by detecting LEV in commercial eye drops, honey, and tap water with satisfactory recoveries (96.0–108.0%). Significance and novelty This work presents the first application of Ce-doped Cu-MOF/β-CD/MWCNTs-COOH composites for the enantioselective electrochemical sensing of ofloxacin, a chiral fluoroquinolone antibiotic. The synergistic combination of high conductivity, abundant catalytic sites, and stereospecific host-guest recognition provides a generalizable strategy for high-performance enantioselective sensing, with promising applications in pharmaceutical, food, and environmental analysis.
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RARE METALS
·
2026
·Vol. 45
·期 7
·e70428
·
IF 12.1
Meiling Han,Hui Hui,Yi Yang,Yan Wang,Danli Zhou,Yuebin Xi,Yu Jing,Pingxian Feng,Fan-Gong Kong,Huan Wang,Weijun Yang
en This link goes to a English section zh This link goes to a Chinese section Lignin is a natural non-toxic polyphenolic macromolecule, which shows inherent antioxidant and broad-spectrum antibacterial activities. However, due to the low content and the high bond dissociation energy of phenolic hydroxyl groups (Ar-O·H), its antioxidant and antibacterial activities are relatively weak, which limits its application as a highly bioactive biomaterial. In this study, different contents of dopamine (DA) were grafted onto the lignin nanoparticles (LNP) via a simple Mannich reaction to prepare LNP- x DA nanomaterial. LNP-1DA has an antibacterial rate of up to 99% against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ), and the antioxidant activity is 40% higher than that of LNP. Meanwhile, both LNP and LNP-DA have excellent blood and cell compatibility, whereas the hemolysis rate was less than 1% ( p < 0.001) and the cell viability for CCK-8 was higher than 95% ( p > 0.05). In order to further investigate its potential application as the bioactive wound-dressing additive, PVA/LNP-DA/Zn hydrogels were prepared by uniformly dispersing LNP-1DA in the Polyvinyl Alcohol (PVA) aqueous solution via the freezing-thawing approach, followed by immersing into the ZnCl 2 solution via an equilibrium method. The synergistic effects of LNP-1DA and Zn 2+ coordination endowed the hydrogels with improved mechanical properties (tensile strength up to 37.9 KPa) and antibacterial activity (antibacterial rate of 99%). This work will be of significance for the high-value utilization of lignin and the development of a novel bioactive material. 摘要 en This link goes to a English section zh This link goes to a Chinese section 木质素是一种天然无毒的多酚类大分子 , 具有固有的抗氧化和广谱抗菌活性。然而 , 由于其含量较低以及酚羟基 ( Ar-O·H ) 键解离能较高 , 导致其抗氧化和抗菌活性相对较弱 , 限制了其作为高生物活性生物材料的应用。本研究通过简单的曼尼希反应 , 将不同含量的多巴胺 ( DA ) 接枝到木质素纳米颗粒 ( LNP ) 上 , 制备出LNP-xDA纳米材料。LNP-1DA 对大肠杆菌 ( E. coli ) 和金黄色葡萄球菌 ( S . aureus ) 的抗菌率高达99% , 其抗氧化活性比LNP高出40%。同时 , LNP和LNP-DA均表现出优异的血液和细胞相容性 , 溶血率低于1% ( p < 0.001 ) , CCK-8检测的细胞存活率高于95% ( p > 0.05 ) 。为进一步探究其作为生物活性敷料添加剂的应用潜力 , 采用冷冻-解冻法将LNP-1DA均匀分散于聚乙烯醇 ( PVA ) 水溶液中 , 再通过平衡法浸入ZnCl₂溶液 , 制备了PVA/LNP-DA/Zn水凝胶。LNP-1DA与Zn²⁺配位产生的协同效应显著提升了水凝胶的力学性能 ( 拉伸强度达37.9 kPa ) 和抗菌活性 ( 抗菌率达99% ) 。本研究对木质素的高值化利用及新型生物活性材料的开发具有重要意义。
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Zhuo-Feng Gan,Jia-Ying Li,Ying-Zhi Yao,Hui-Min Lu,Xi-Yang Chen,Ming-Shen Zhu,Duried Alwazeer,Xin-An Zeng,Lan Liao
Carbonyl mapping linked Fenton oxidation to refrigerated MP deterioration. • H₂ modulated the upstream redox microenvironment and reduced the detectable DMPO-OH signal. • H₂ attenuated marker-dependent oxidative modification in tilapia MPs. • H₂ attenuated aggregation, colloidal destabilization, and conformational reorganization. Hydroxyl radical (·OH)-driven oxidation of myofibrillar proteins (MPs) contributes to quality deterioration in refrigerated fish, yet storage-referenced mitigation strategies remain limited. Herein, we established a storage-referenced oxidation framework for tilapia MPs by carbonyl-based equivalence fitting a Fenton-type ·OH-generating system and carbonyl accumulation in vacuum-packaged tilapia muscle during extended refrigerated storage at 4 °C. Within this framework, weeks 1, 4, 6, and 7 corresponded to 20, 50, 80, and 90 mM H₂O₂, respectively, with a maximum absolute prediction error of 8.6%, and 90 mM H₂O₂ was selected as the representative high-oxidation condition. Under this condition, H₂ shifted the system toward a more reducing redox status and reduced the detectable DMPO-OH signal to 24.56% of the 90 mM H₂O₂ control, accompanied by marker-dependent protection against oxidative modification, antioxidant-related readout loss, colloidal destabilization, and secondary-structure deterioration. These findings provide a mechanistic basis for H₂-based reductive preservation of aquatic muscle foods. Download: Download high-res image (202KB) Download: Download full-size image
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危险品化学品经营许可证（带存储）