BMC Oral Health
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2025
·Vol. 25
·期 1
·1-16
·
IF 3.1
Guo Tiantian,Wang Dai,Gao Sherry Shiqian
Background To investigate the antibiofilm effect and mechanism of the silver nanowire (AgNW)-modified glass ionomer cement (GIC) against multi-species oral biofilm, and to examine the mechanical and biochemical properties of this novel GIC material. Methods Conventional GIC was incorporated with different concentrations of AgNW and silver nanoparticles (AgNP). Multi-species biofilms of Streptococcus mutans , Streptococcus sobrinus , Lactobacillus fermentum , and Lactobacillus rhamnosus were cultured for 72 h on GIC specimens. Scanning electron microscopy (SEM) was adopted to examine the accumulation of biofilm on GIC surfaces. A live/dead assay was performed to assess the viability of bacteria. Extracellular polysaccharides (EPS) were labelled with Alexa Fluor 647-labelled dextran conjugate and then observed by a confocal laser scanning microscope (CLSM). The D/L-Lactic Acid Assay Kit was used to evaluate the lactic acid production of the multi-species biofilms. Compressive strength, surface roughness, hardness, and wettability were measured by a universal testing machine, an atomic force microscope (AFM), a Vickers microhardness tester, and a contact angle meter, respectively. Colour stability and fluoride release of GIC specimens were assessed by VITA Easyshade® V and ion chromatography. Cell counting kit-8 (CCK-8) was used to study cytotoxicity. Results SEM images showed that fewer biofilms were accumulated on the AgNW-GIC surfaces. The live/dead assay showed that the ratio of live bacteria was significantly lower in AgNW-GIC groups than in conventional GIC (5.8% vs. 100%, p < 0.0001). The EPS production was significantly less in AgNW-GIC groups compared to conventional GIC ( p < 0.0001). There is no difference between groups regarding lactic acid production and fluoride release. The mechanical strength including compressive strength, surface roughness, hardness, and wettability were comparable between groups. The colour change between AgNW-GIC and conventional GIC was much milder than that between AgNP-GIC and conventional GIC. The results of cytotoxicity showed no significant differences in cell viability between groups. Conclusions This study demonstrated that AgNW-GIC had an excellent antibiofilm effect against multi-species oral biofilm, comparable mechanical and biochemical properties, and did not significantly affect the colour stability of GIC. The antibiofilm mechanism of AgNW-GIC may be related to inhibiting the viability and EPS production of bacteria.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-20
·
IF 10.6
Liu Lisha,Deng Dashi,Li Chenchen,Huang Guixiao,Zhang Wenjuan,Liang Ting,Liang Rui,Liang Mingkang,Su Yilin,Lin Chongyang,Li Guangzhi,Wu Song
Rheumatoid arthritis (RA) involves chronic joint inflammation. Combining acupuncture and medication for RA treatment faces challenges like spatiotemporal variability, limited drug loading in acupuncture needles, and premature or untargeted drug release. Here, we designed a new type of tubular acupuncture needles, with an etched hollow honeycomb-like structure to enable the high loading of therapeutics, integrating the traditional acupuncture and drug repository into an all-in-one therapeutic platform. In these proof-of-concept experiments, we fabricated injectable hollow honeycomb electroacupuncture needles (HC-EA) loaded with melittin hydrogel (MLT-Gel), enabling the combination treatment of acupuncture stimulation and melittin therapy in a spatiotemporally synchronous manner. Since the RA microenvironment is mildly acidic, the acid-responsive chitosan (CS)/sodium beta-glycerophosphate (β-GP)/ hyaluronic acid (HA) composited hydrogel (CS/GP/HA) was utilized to perform acupuncture stimulation and achieve the targeted release of injected therapeutics into the specific lesion site. Testing our therapeutic platform involved a mouse model of RA and bioinformatics analysis. MLT-Gel@HC-EA treatment restored Th17/Treg-mediated immunity balance, reduced inflammatory factor release (TNF-α, IL-6, IL-1β), and alleviated inflammation at the lesion site. This novel combination of modified acupuncture needle and medication, specifically melittin hydrogel, holds promise as a therapeutic strategy for RA treatment. Graphical abstract
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
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IF 3.8
Zhang Shuang,Jiang Yunhao,Lei Wenli,Zhai Yueming,Liu Juejing,Lyu Xingyi,Li Tao,Guo Xiaofeng,Zhao Yuanmeng,Shan Changsheng,Niu Li
The development of cost-effective and highly efficient electrocatalysts is critical to help electrochemical non-enzymatic sensors achieve high performance. Here, a new class of catalyst, Ru single atoms confined on Cu nanotubes as a single-atom alloy (Ru 1 Cu NTs), with a unique electronic structure and property, was developed to construct a novel electrochemical non-enzymatic glucose sensor for the first time. The Ru 1 Cu NTs with a diameter of about 24.0 nm showed a much lower oxidation potential (0.38 V) and 9.0-fold higher response (66.5 μA) current than Cu nanowires (Cu NWs, oxidation potential 0.47 V and current 7.4 μA) for glucose electrocatalysis. Moreover, as an electrochemical non-enzymatic glucose sensor, Ru 1 Cu NTs not only exhibited twofold higher sensitivity (54.9 μA mM −1 cm −2 ) and wider linear range (0.5–8 mM) than Cu NWs, but also showed a low detection limit (5.0 μM), excellent selectivity, and great stability. According to theoretical calculation results, the outstanding catalytic and sensing performance of Ru 1 Cu NTs could be ascribed to the upshift of the d-band center that helped promote glucose adsorption. This work presents a new avenue for developing highly active catalysts for electrochemical non-enzymatic sensors. Graphical Abstract
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Scientific Reports
·
2024
·Vol. 14
·期 1
·1-14
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IF 3.8
Ye Jian,Shi Chenyang,Lan Jian,Chen Qingqing,Si Qin,Xu Panpan,Zhang Xijiang,Zheng Cheng
Rapid and safe hemostasis is crucial for the survival of bleeding patients in prehospital care. It is urgent to develop high performance hemostatic material to control the massive hemorrhage in the military field and accidental trauma. In this work, an efficient protein hemostat of thrombin was immobilized onto commercial gauze, which was mediated by self-polymerization and anchoring of tannic acid (TA). Through TA treatment, the efficient immobilization of thrombin was achieved, preserving both the biological activity of thrombin and the physical properties of the dressing, including absorbency, breathability, and mechanical performance. Moreover, in the presence of TA coating and thrombin, Gau@TA/Thr could obviously shortened clotting time and enriched blood components such as plasma proteins, platelets, and red blood cells, thereby exhibiting an enhanced in vitro coagulation effect. In SD rat liver volume defect and artery transection hemorrhage models, Gau@TA/Thr still had outstanding hemostatic performance. Besides, the Gau@TA/Thr gauze had inherent antibacterial property and demonstrated excellent biocompatibility. All results suggested that Gau@TA/Thr would be a potential candidate for treating uncontrollable hemorrhage in prehospital care.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2024
·Vol. 31
·期 18
·27388-27402
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IF 0
Lan Jinghao,Yan Hengwei,Liu Zhanwei,Ma Wenhui
In aluminum electrolysis, the iron-rich cover material is formed on the cover material and the steel rod connecting the carbon anode. Due to the high iron content in the iron-rich cover material, it differs from traditional cover material and thus requires harmless recycling and treatment. A process was proposed and used in this study to recovery F, Al, and Fe elements from the iron-rich cover material. This process involved aluminum sulfate solution leaching for fluorine recovery and alkali-acid synergistic leaching for α-Al 2 O 3 and Fe 2 O 3 recovery were obtained. The optimal leaching rates for F, Na, Ca, Fe, and Si were 93.92, 96.25, 94.53, 4.48, and 28.87%, respectively. The leaching solution and leaching residue were obtained. The leaching solution was neutralized to obtain the aluminum hydroxide fluoride hydrate (AHFH, AlF 1.5 (OH) 1.5 ·(H 2 O) 0.375 ). AHFH was calcined to form a mixture of AlF 3 and Al 2 O 3 with a purity of 96.14%. The overall recovery rate of F in the entire process was 92.36%. Additionally, the leaching residue was sequentially leached with alkali and acid to obtain the acid leach residue α-Al 2 O 3 . The pH of the acid-leached solution was adjusted to produce a black-brown precipitate, which was converted to Fe 2 O 3 under a high-temperature calcination, and the recovery rate of Fe in the whole process was 94.54%. Therefore, this study provides a new method for recovering F, Al, and Fe in iron-rich cover material, enabling the utilization of aluminum hazardous waste sources.
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Probiotics and Antimicrobial Proteins
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2025
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IF 4.4
Zhao Yuting,Xie Weiqi,Duan Jingyi,Li Fei
Limosilactobacillus reuteri DSM 17938 ( L. reuteri DSM 17938) was one of the most widely used probiotics in humans for gastrointestinal disorders, but few studies have investigated its role in drug-induced liver injury (DILI). Here, we evaluated the efficacy of L. reuteri DSM 17938 using a mouse model of DILI induced by triptolide. Pregavage of L. reuteri DSM 17938 for 1 week remarkably lowered hepatic inflammatory cytokines level and oxidative stress, with diminished serum alanine transaminase and aspartate aminotransferase levels. Metabolomics and RT-qPCR analysis confirmed its ability in ameliorating TP-disrupted hepatic fatty acid β oxidation. Genome annotation of L. reuteri showed its ability to modulate energy metabolism. Targeted metabolomics demonstrated that L. reuteri DSM 17938 modified the short fatty acid profiles in cecum, especially enhancing propionate levels. Further experiments found that L. reuteri DSM 17938 can activate AMPK signaling by upregulating gut microbiota–derived propionate level, thus restoring impaired mitochondrial biogenesis and energy supply processes to recover energy homeostasis, which leads to diminished ROS production and oxidative stress injury in hepatocytes. Besides, AMPK inhibitor dorsomorphin abolished all the effects on propionate protecting mitochondria and energy metabolism. This study established probiotic therapy of L. reuteri DSM 17938 as a preventive intervention for DILI in clinical. We also revealed that L. reuteri DSM 17938 can activate AMPK signaling by propionate, facilitating a deeper understanding of the action mechanism of L. reuteri DSM 17938 against acute liver injury and contributing to the development of its postbiotics and wider applications. Graphical Mechanism illustration of Limosilactobacillus reuteri DSM 17938 alleviating TP-induced liver injury by enhancing AMPK signaling pathway via propionate (Created with Biorender. com).
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MOLECULAR MEDICINE
·
2024
·Vol. 30
·期 1
·1-16
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IF 6
Xie Bin,Chen Qiong,Dai Ziyu,Jiang Chen,Chen Xi
Background Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory lung disease associated with high morbidity and mortality worldwide. Oxidative injury and mitochondrial dysfunction in the airway epithelium are major events in COPD progression.Methods and resultsThe therapeutic effects of Progesterone (P4) were investigated in vivo and in vitro in this study. In vivo, in a cigarette smoke (CS) exposure-induced COPD mouse model, P4 treatment significantly ameliorated CS exposure-induced physiological and pathological characteristics, including inflammatory cell infiltration and oxidative injury, in a dose-dependent manner. The c-MYC/SIRT1/PGC-1α pathway is involved in the protective function of P4 against CS-induced COPD. In vitro, P4 co-treatment significantly ameliorated H2O2-induced oxidative injury and mitochondrial dysfunctions by promoting cell proliferation, increasing mitochondrial membrane potential, decreasing ROS levels and apoptosis, and increasing ATP content. Moreover, P4 co-treatment partially attenuated H2O2-caused inhibition in Nrf1, Tfam, Mfn1, PGR-B, c-MYC, SIRT1, and PGC-1α levels. In BEAS-2B and ASM cells, the c-MYC/SIRT1 axis regulated P4’s protective effects against H2O2-induced oxidative injury and mitochondrial dysfunctions.Conclusion P4 activates the c-MYC/SIRT1 axis, ameliorating CS-induced COPD and protecting both airway epithelial cells and smooth muscle cells against H2O2-induced oxidative damage. PGC-1α and downstream mitochondrial signaling pathways might be involved.
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Scientific Reports
·
2024
·Vol. 14
·期 1
·1-10
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IF 3.8
Tong Luqing,Ye Ke,Chen Qun,Wang Xiaoxi,Hu Chi,Xu Qingsheng,Zhou Lihui,Zhan Renya,Tong Ying
Gamma knife radiosurgery (GKRS) is recommended as the first-line treatment for brain metastases of lung adenocarcinoma (LUAD) in many guidelines, but its specific mechanism is unclear. We aimed to study the changes in the proteome of brain metastases of LUAD in response to the hyperacute phase of GKRS and further explore the mechanism of differentially expressed proteins (DEPs). Cancer tissues were collected from a clinical trial for neoadjuvant stereotactic radiosurgery before surgical resection of large brain metastases (ChiCTR2000038995). Five brain metastasis tissues of LUAD were collected within 24 h after GKRS. Five brain metastasis tissues without radiotherapy were collected as control samples. Proteomics analysis showed that 163 proteins were upregulated and 25 proteins were downregulated. GO and KEGG enrichment analyses showed that the DEPs were closely related to ribosomes. Fifty-three of 70 ribosomal proteins were significantly overexpressed, while none of them were underexpressed. The risk score constructed from 7 upregulated ribosomal proteins (RPL4, RPS19, RPS16, RPLP0, RPS2, RPS26 and RPS25) was an independent risk factor for the survival time of LUAD patients. Overexpression of ribosomal proteins may represent a desperate response to lethal radiotherapy. We propose that targeted inhibition of these ribosomal proteins may enhance the efficacy of GKRS.
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PHARMACEUTICAL CHEMISTRY JOURNAL
·
2024
·Vol. 58
·期 5
·765-774
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IF 0.8
Qiu Shouliang,Deng Xuezhen,Qian Kun,Yang Jie,Ma Yuexing
Drug-drug cocrystals can improve the physical and pharmaceutical properties of drugs and have been widely studied by many researchers. A new pharmaceutical cocrystal [C 16 H 18 FN 3 O 3 ][C 16 H 14 O 6 ] · 2(C 2 H 5 OH) (HSP-NOR) was synthesized by hesperetin (HSP) and norfloxacin (NOR) with the aim of improving the antibacterial activity and physicochemical properties. The single crystal structure shows that the asymmetric unit comprises both hesperetin and norfloxacin, as well as two ethanol molecules, in which norfloxacin exists as a zwitterion. The cocrystal formation improves the solubilities of hesperetin and norfloxacin in pure water. Preliminary antibacterial experiments showed that the antibacterial activities of the cocrystal on Streptococcus pneumoniae , Escherichia coli , Shigella dysenteriae , and Pseudomonas aeruginosa were stronger than those of norfloxacin. The in vivo study of acute toxicity showed that the minimum lethal dose of the cocrystal to mice was >3000 mg kg -1 BW, and there were no signs of toxicity after oral administration at this dosage.
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JOURNAL OF MATERIALS SCIENCE
·
2024
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IF 3.5
Zhang Mengmeng,He Zhaojun,Li Zuojia,Deng Biao,Ren Honghe
Combination of adsorption and photocatalytic degradation is an effective strategy for rapid elimination of organic pollutants from contaminated water. Herein, in order to improve the dispersion of catalyst, increase the reactive sites and facilitate the recovery of catalyst, a magnetic silver phosphate/strontium ferrite/graphene oxide (ASG) ternary composite was constructed through a two-step method. According to PL, UV–vis and photoelectrochemical characterization and adsorption experimental results, the ASG exhibits enhanced light absorption, reduced band gap, increased photocurrent response and improved adsorption ability compared to Ag 3 PO 4 and Ag 3 PO 4 /SrFeO. ASG showed remarkable adsorption–photocatalytic synergistic removal effect on levofloxacin (LEO) and methylene blue (MB). At room temperature, for 20 mg/L of initial LEO concentration, 0.67 g/L of ASG can absorb 80.7% of LEO, while the removal rate increases up to 95.5% under visible light irradiation within 180 min; for 20 mg/L of initial MB concentration, 0.67 g/L of ASG can absorb 100% of MB with 90 min, while the elimination time can be shortened to 45 min through adsorption–photocatalytic synergy. The cyclic experiments showed that the removal rates of LEO and MB by ASG remained above 80% after 5 adsorption–photocatalytic cycles, indicating that ASG has good stability and availability. In addition, a possible mechanism for removing LEO and MB by ASG is proposed based on the free radical capture experimental, UV–vis and photoelectrochemical results, which provides references for the rational design and construction of magnetic multifunctional photocatalysts for the efficient removal of organic contaminants in practical wastewater. Graphical abstract
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RARE METALS
·
2024
·
IF 9.6
Feng Yu-Xiao,Yu Hui-Jun,Lu Tian-Guang,Zheng Zi-Ye,Tian Shuang,Xiang Li,Zhao Shan,Wang Shu-Guang,He Zuo-Li
Metal clusters or even single-atoms dispersed and anchored on the photocatalysts’ surface can enhance photocatalytic performances on organic pollutant oxidation. Here, a simple photoreduction method was used to create atomically dispersed metal single-atoms/clusters (MSCs, M = Cu, Pd, Au and Ag) on P-modulated tubular carbon nitride (TCN). The obtained MSCs@TCN demonstrated excellent photocatalytic performances for the degradation of sulfamethazine (SMZ). In particular, the photocatalyst with 2 wt% Cu loading showed ultrahigh SMZ oxidation efficiency ( k = 0.06110 min −1 ), almost three times that of TCN ( k = 0.02066 min −1 ). It also shows excellent stability in the 5th-cycle measurements. The improved photocatalytic activity of the CuSCs@TCN is ascribed to the synergistic promotion of photogenerated charge separation by Cu single-atoms/clusters as active sites, accelerated charge transfer from bulk TCN to Cu sites through Cu–N x interaction. Meanwhile, the active sites of Cu single-atoms/clusters could promote the production of ·O 2 − , which participates in organic oxidation with strong oxidizing holes (h + ). This strategy paves a new avenue for designing high-performance photocatalysts decorated with metal single-atoms and clusters.
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COLLOID AND POLYMER SCIENCE
·
2024
·
IF 2.2
Cai Yuanpeng,Li Danyang,Peng Shaohua,Liu Hui
Nanogels offered a large surface area for biological conjugation due to their nanoscale size, enabling prolonged circulation in the bloodstream and targeted delivery to specific tissues. CMC/POM nanogels composed of 2-(2-(2-methoxyethoxy)ethoxy)ethyl methacrylate (MEO 3 MA), methacrylic acid (MAA), and natural biopolymer carboxymethyl cellulose (CMC) were synthesized via free radical polymerization. The chemical structures of CMC/POM nanogels were characterized using several techniques, and the responsive behaviors under temperature and pH variations were reflected by changes in turbidity and particle size. The results demonstrated that CMC/POM nanogels exhibited significant changes in particle size and turbidity around 38 °C and under different pH conditions. Additionally, the unique microstructure of CMC/POM nanogels made them promising for drug delivery through injection therapy. With doxorubicin (DOX) as a model drug, the nanogels could achieve a maximum drug loading content of 31.47% and drug loading efficiency of 62.93%. During in vitro controlled drug releasing, the release efficiency was up to 92.20% within 24 h at T = 45 °C and pH = 3.0. The cytotoxicity assays confirmed that CMC/POM nanogels possessed superior biocompatibility and drug-loaded CMC/POM nanogels effectively inhibited the activity of cancer cells. CMC/POM nanogels displayed remarkable potential as intelligent drug delivery systems, and they were expected to play an essential role in the field of anticancer therapies. Graphical
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