CURRENT MICROBIOLOGY
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2025
·Vol. 82
·期 9
·1-18
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IF 2.6
Xu Wenyi,Guan Yinghua,Tian Liping,Fu Yu,Zhang Yinghui,Liu Yanhong,Hu Yanfei,Zhao Bowen,Wu Chongming
The global prevalence of type 2 diabetes mellitus (T2DM) is currently surging, posing significant health and socioeconomic burdens. Probiotics have emerged as promising interventions due to their safety and potential metabolic benefits. Gut microbiota plays a critical role in the pathophysiology of metabolic diseases in humans, offering valuable insights for therapeutic strategies such as probiotics. Here, aiming to identify novel probiotics from the human gut microbes to combat T2DM, a total of 203 human intestinal bacterial strains were screened in vitro to evaluate their enzymatic inhibition of α-glucosidase and α-amylase, in which Lacticaseibacillus paracasei Glu-07 (= QH-142 = CGMCC 23796) exhibited the highest inhibitory efficacy (81.05% and 72.56% for α-glucosidase and α-amylase, respectively). In HepG2 cells, L. paracasei Glu-07 elevated glucose uptake and consumption by 22.4% and 85.8%. Oral administration of strain Glu-07 in diabetic mice significantly improved hyperglycemia, hyperlipidemia, and restored the structure and composition of gut microbiota toward a healthier profile. Mechanistically, strain Glu-07 activated the AMPK-AKT1-GSK3β/FOXO1 signaling pathways by modulating the expression of key genes, enhanced glycolysis and fatty acid ꞵ-oxidation, and inhibited gluconeogenesis and lipogenesis. It also stimulated GLP-1 secretion, potentially via triggering the GPR41/43-GLP-1 axis, and increased the abundance of beneficial gut microbes, such as Akkermansia muciniphila (~ threefold). The dual regulations synergistically support strain Glu-07 as a promising probiotic candidate for T2DM management.
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BIOCHEMICAL SYSTEMATICS AND ECOLOGY
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2025
·
IF 2
Li-Juan Hu,Xian-Sheng Ye,Kuan Lin,Jie Gao,De-Sen Yang,Guo-Ping Gan,Xi-Ji Shu,Wei Liu,You-Hua Yang
One previously undescribed 2-arylbenzofuran derivative ( 1 ), three stilbenes ( 2 - 4 ), three acetophenone glycosides ( 5 – 7 ), three phenylpropanoids ( 8 – 10 ), and eight flavonoids ( 11 – 18 ) were isolated from the rhizome of Smilax china L. in this study. NMR spectra elucidated their structures and were compared with previously reported data. Compounds 1 , 5 – 7 , 10 , and 18 were identified for the first time in Smilax china L. The cytotoxicity of the isolated compounds was detected on HeLa cells by the CCK-8 method, and compound 4 was found to possess the highest cytotoxic activity with an IC 50 value of 34.81 ± 1.50 μg/mL. Further studies revealed that the cytotoxic effect of compound 4 on HeLa cells was related to apoptosis and the cell cycle. In addition, the chemotaxonomic significance of the isolated compounds was discussed.
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ELECTROCHIMICA ACTA
·
2025
·
IF 5.6
Tao Huang,Yongwei Chen,Xiangzhen Zheng,Ying Pan,Maoxiang Wu
Layered lithium-rich oxides(LRO) materials have garnered significant attention due to their high reversible capacity and broad operating voltage range. However, the practical application of LRO materials is still restricted by the serious interfacial instability and surface structure reconstruction during long-term cyclic test. In this study, we use 2-(Trimethoxysilyl)ethyl methacrylate (TSM) as a multifunctional electrolyte additive to enhance the electrochemical performance of the Li/LRO cell. The capacity retention of the Li/LRO cell improves significantly from 67.2 % to 85.8 % after 100 cycles at 0.5C (1C = 300 mAh g -1 ) between 2.0 and 4.8 V with the addition of 2 % TSM. Under harsh conditions (with the addition of 1000 ppm H 2 O to the electrolytes), Li/LRO cell with TSM additives maintains the capacity retention of 82.5 % after 100 cycles, which is significantly higher than the cell without additives (38.0 %). Both theoretical calculations and experiments results demonstrate that TSM exhibits higher oxidation activity than the solvents, forming a uniform cathode electrolyte interphase (CEI) on the LRO surface. The TSM-derived CEI layer effectively mitigates the interfacial parasitic reactions and prevents structural degradation of LRO. Furthermore, theoretical calculations and nuclear magnetic resonance (NMR) results confirm that TSM effectively eliminates water (H 2 O) and hydrofluoric acid (HF), mitigating their adverse effects.
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Food & Function
·
2025
·
IF 5.4
Ting Liu,Qiang Meng,Yijun Zhang,Mengxi Yu,Jianming Ye,Wei Song,Yane Luo,Tianli Yue
The gut microbiota and its associated micro-ecosystem were closely related to the onset and development of ulcerative colitis (UC). We found that Corynebacterium glutamicum (C. glutamicum) helps rebuild gut eubiosis from diabetes dysbiosis, while its effects on UC remain unknown. This study aims to investigate the therapeutic effects and mechanisms of C. glutamicum on UC. In this study, C. glutamicum was encapsulated with thiolated hyaluronic acid (HA-SH) to form a hydrogel termed as CG-HA-SH. The adhesion and distribution of CG-HA-SH in the intestine were evaluated, along with its therapeutic effects on UC mice, including its impact on the gut microbiota. Additionally, changes in short-chain fatty acids (SCFAs) in the intestines of UC mice were analyzed, and RNA sequencing (RNA-Seq) was employed to investigate the mechanisms by which C. glutamicum alleviated inflammation. HA-SH enhanced the resistance of C. glutamicum in gastric and intestinal fluids, providing approximately 12 hours of adhesion at colitis inflammation sites. C. glutamicum reduced the levels of pro-inflammatory factors like IL-1β (by 97.31%) and TNF-α (by 90.10%) while increasing anti-inflammatory IL-10 (by 197.59%) in the colon. It also increased the abundance of E. fissicatena, Muribaculum, and butyrate, enhanced intestinal tight junctions (OCC, by 318.93%) and mucus barrier (MUC2, by 515.93%). The mRNA levels of cIAP1/2 decreased by 4.33 folds, their protein expression levels were reduced by 36.97% correspondingly. The enrichment of TNF pathway was the most significant. Therefore, C. glutamicum exhibited remarkable efficacy in alleviating inflammation and reshaping dysbiotic gut microbiota by downregulating the cIAP1/2-mediated TNF signaling pathway and NF-κB signaling pathway. cIAP1 and cIAP2 might serve as effective therapeutic targets.
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CHEMICAL ENGINEERING SCIENCE
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2025
·
IF 4.3
Jiqing Rao,Xin Tian,Yuze Sun,Honghai Wang,Rui Wang,Chunli Li,Jiapeng Liu
2-methyltetrahydrofuran (2-MeTHF) and acetonitrile (ACN) are widely used as solvents in the pharmaceutical industry. As 2-MeTHF and ACN form azeotrope that cannot be separated via regular distillation. Therefore, in this study, the molecular mechanism, experiment-based extractant screening, and process design for the separation of the 2-MeTHF-ACN azeotrope were investigated. According to Fourier-transform infrared spectroscopy and density functional theory, the azeotropy was destroyed via the formation of hydrogen bonds between the extractant dimethyl sulfoxide, 2-MeTHF, and ACN. Vapor–liquid phase equilibrium experiments validated the screening results and develop a thermodynamic model of the binary system. Batch distillation experiments obtained 2-MeTHF with a mass purity of 99.6 wt% and ACN with a mass purity of 99.8 wt%. The ED process was designed using Aspen Plus and improved via the integration of the recovered waste heat (ED-HI). A sequential iterative approach was used to minimize the total annual cost (TAC). The ED-HI process resulted in reductions in the TAC, reboiler heat load, and CO 2 emissions of 20.06 %, 25.77 %, and 22.77 %, respectively, compared to those of the ED process. This study provides valuable insight into the effective separation of 2-MeTHF and ACN.
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Frontiers in Genetics
·
2025
·
IF 2.8
Minglu Zhang,Jianbo He,Rao Fu,Guojian Bao,Yijun Lu,Zechuan Zhang,Jiawu Yan,Jialu Ding,Fei Yang,Beicheng Sun
Introduction: Small nucleolar RNA (snoRNA) mediates RNA modifications, including 2′-O-methylation (Nm) and pseudouridine (Ψ), which has been proven to impact tumor progression. However, the role of snoRNA in the epigenetics of tumors remains poorly understood due to the lack of sufficiently effective experimental methods to identify snoRNA targets. Here, we identified SNORD13H, a C/D box snoRNA, as being downregulated in hepatocellular carcinoma (HCC), and its low expression was associated with HCC development.Methods: To elucidate specific roles of SNORD13H in HCC, we used a comprehensive array of methodologies, including flow cytometry, xenograft mouse model, reverse transcription at low dNTP concentration followed by PCR (RTL-P) assay, and surface sensing of translation (SUnSET) assay.Results: In this study, we first demonstrated that reduced SNORD13H serves as a biomarker for HCC, facilitating cellular proliferation. SNORD13H mediates 2′-O-methylations of 18S rRNA and RAS mRNA, thereby enhancing translation efficiency and regulating RAS protein levels in HCC. The diminution of SNORD13H activates the RAS pathway, contributing to the progression of HCC.Discussion: Our study establishes SNORD13H as a dual-function regulator in HCC progression. Furthermore, our findings indicate that SNORD13H is detectable in plasma, highlighting its potential utility in tumor screening.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2025
·
IF 4.9
Jianhong Liu,Yujie Han,Xin He,Xinghua Zhao
Type 2 diabetes (T2DM), a chronic metabolic disorder driven by insulin resistance or deficiency, necessitates novel therapeutic strategies. Piperine (PIP), a bioactive alkaloid from Piper nigrum and Piper longum , exhibits antidiabetic potential but suffers from poor solubility and bioavailability. This study developed two cocrystals, PIP-3,5-DHBA·H 2 O and PIP-2,6-DHBA, to address these limitations. Structural characterization via SCXRD, PXRD, DSC-TGA, FT-IR, and optical microscopy confirmed cocrystal formation. Pharmacokinetic and therapeutic evaluations focused on PIP-3,5-DHBA·H 2 O in STZ-induced diabetic C57BL/6J mice over 10 weeks, assessing metabolic parameters, pancreatic histopathology, and gut microbiota. Results revealed enhanced solubility for both cocrystals, with PIP-3,5-DHBA·H 2 O displaying superior bioavailability (2.8-fold increases in C max and AUC 0–24 ). Treatment significantly reduced fasting glucose, TG, TC, and LDL-C while elevating HDL-C. Gut microbiota analysis demonstrated decreased Firmicutes / Bacteroidetes ratio and enriched beneficial genera ( Coprococcus , Akkermansia ). Mechanistically, the cocrystal improved β-cell function and metabolic regulation. These findings validate cocrystallization as a viable strategy to overcome PIP's physicochemical limitations, offering a dual-functional approach for T2DM management through enhanced bioavailability, metabolic restoration, and microbiome modulation.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2025
·
IF 8.3
Dawei Lu,Wen Meng,Xiaowei Wen,Feng Li,Taohai Li
Herein, an Ag/Ni(dmgH) 2 photocatalyst was constructed through a controllable silver modification strategy. The combination of the local surface plasmon resonance (LSPR) effect of Ag nanoparticles and the synergistic catalytic effect of Schottky junctions significantly enhanced photogenerated carrier separation. Under optimized conditions, the Ag/Ni(dmgH) 2 system achieved a remarkable hydrogen evolution rate of 922.2 μmol/g/h under visible light, approximately 16.7 times higher than the unmodified system. Furthermore, the Ag/Ni(dmgH) 2 film fabricated via screen-printing technology effectively utilized enhanced light absorption from LSPR and efficient interfacial electron transport from Schottky junctions, achieving degradation rates of 98.9 % for Rhodamine B and 86.9 % for Quinoline Blue. The film retained 86.9 % of its activity after four cycles, demonstrating excellent recyclability. This work highlights the dual application potential of Ni(dmgH) 2 materials for photocatalytic energy conversion and environmental remediation.
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JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Duo Xu,Bowen Du,Yantian Ji,Huimin Sun,Tiecheng Wang,Xianqiang Yin
The study examined the transport behavior of the 2-aryl propionic acid (2-APA) chiral pharmaceutical enantiomers by means of a laboratory-scale saturated quartz sand column experiment. Four typical of 2-APA and their enantiomers were selected for the study under different types of chiral organic acids (COAs)-mediated effects. Differences in the transport of the 2-APA enantiomeric pairs have been identified in response to various pH, types of COAs, and enantiomeric structures of COAs. Redundancy analysis identified the factors responsible for the largest differences in transport of 2-APA enantiomeric pairs, while spectroscopic characterization and density function theory (DFT) studies elucidated the underlying mechanisms contributing to the differences in transport of enantiomeric pairs. Obvious correlations among homochirality or heterochirality between COAs and 2-APA enantiomeric pairs were observed for changes in the mobility of 2-APA. The results indicate widespread COAs significantly affect the transport behavior of chiral man-made chemicals, suggesting more attention is needed to fill the gap in the perception of the transport behavior of chiral compounds.
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BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Chunxia Chen,Shujie Duan,Jianing Ji,Mengli Wu,Zhan Yang,Mengyang Cai,Menghan Xue,Lanju Wang,Rui Chen,Sima Yaron,Keying Guo,Sergio Benardini,Zhizeng Wang,Yang Luo
Conventional point-of-care lateral flow immunoassays are characterized by an antibody-tagged probe irregular coupling that can limit sensitivity and require a long assay’s time. We synthesized polyethylene glycol-modified selenium nanoparticles (PEG-SeNPs) by template method and developed a strategy to set antibody probes targeted and orderly by using PEG-SeNPs. Synthesized PEG-SeNPs with high stability could immobilize antibodies in the “stand-up” orientation, resulting in a faster detection time of less than 1 min by direct observer visualization without instruments or equipment. Results showed that SARS-CoV-2 antibody could be systematically structured on the chip, resulting in a detection limit of 10 pg/mL, significantly higher than conventional chips. The new device has been validated on 192 clinical samples and we found 100% negative coincidence, 93.94% positive coincidence, and 95.83% overall coincidence with reverse transcriptional PCR test. The orderly arrayed probe’s stability allowed to detect throat swabs, saliva, serum, fingertip blood samples, and mutant strains without cross-reactivity with common respiratory viruses or pathogenic strains, demonstrating promising potential for a universal colorimetric platform for ultrafast field-deployable diagnostics.
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Drug Design Development and Therapy
·
2024
·
IF 4.7
Yong-Yu Yang,Rong-Rong Deng,Da-Xiong Xiang
Background Ferroptosis plays a crucial role in the occurrence and development of cerebral ischemia-reperfusion (I/R) injury and is regulated by mitogen-activated protein kinase 1/2 (ERK1/2). In China, Naodesheng Pills (NDSP) are prescribed to prevent and treat cerebrosclerosis and stroke. However, the protective effects and mechanism of action of NDSP against cerebral I/R-induced ferroptosis remain unclear. We investigated whether NDSP exerts its protective effects against I/R injury by regulating ferroptosis and aimed to elucidate the underlying mechanisms.Methods The efficacy of NDSP was evaluated using a Sprague-Dawley rat model of middle cerebral artery occlusion and an in vitro oxygen-glucose deprivation/reoxygenation (OGD/R) model. Brain injury was assessed using 2,3,5-triphenyltetrazolium chloride (TTC), hematoxylin and eosin staining, Nissl staining, and neurological scoring. Western blotting was performed to determine the expression levels of glutathione peroxidase 4 (GPX4), divalent metal-ion transporter-1 (DMT1), solute carrier family 7 member 11 (SLC7A11), and transferrin receptor 1 (TFR1). Iron levels, oxidative stress, and mitochondrial morphology were also evaluated. Network pharmacology was used to assess the associated mechanisms.Results NDSP (1.08 g/kg) significantly improved cerebral infarct area, cerebral water content, neurological scores, and cerebral tissue damage. Furthermore, NDSP inhibited I/R- and OGD/R-induced ferroptosis, as evidenced by the increased protein expression of GPX4 and SLC7A11, suppression of TFR1 and DMT1, and an overall reduction in oxidative stress and Fe2+ levels. The protective effects of NDSP in vitro were abolished by the GPX4 inhibitor RSL3. Network pharmacology analysis revealed that ERK1/2 was the core target gene and that NDSP reduced the amount of phosphorylated ERK1/2.Conclusion NDSP exerts its protective effects against I/R by inhibiting cerebral I/R-induced ferroptosis, and this mechanism is associated with the regulation of ferroptosis via the ERK1/2 signaling pathway.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2024
·Vol. 40
·期 4
·730-736
·
IF 3.1
Hu Lin,Tong Shengrui,Xu Yanyong,Zhang Hailiang,Yu Shanshan,Chen Meifang,Ge Maofa
Unsaturated ketones are typical oxygenated volatile organic compounds (OVOCs) with high reactivity, and are important precursors in air pollution. The sources of OVOCs are complex and include direct emissions and secondary oxidation formation of VOCs in the atmosphere. 2-Cyclohexen-1-one is a widespread substance, and is derived from the industrial catalytic oxidation of cyclohexene. In this paper, we investigated the rate constants of the chemical reactions of 2-cyclohexen-1-one with NO 3 radicals, which is (7.25±0.29)×10 −15 cm 3 ·molecule −1 ·s −1 at 298 K and under 1 atm (1 atm=101325Pa). It supplemented the kinetics of NO 3 radicals database, and revealed its effects in the nighttime atmosphere. In addition, the reaction products of 2-cyclohexen-1-one with NO 3 radicals were detected by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), which revealed a series of nitrate esters in the composition of the secondary organic aerosol (SOA), which may reduce atmospheric visibility. Finally, the possible pathways for the generation of the products were developed.
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危险品化学品经营许可证（带存储）