Journal of Cell Communication and Signaling
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2023
·
IF 4.1
Zhao Ye,Wang Huiling,He Tianhua,Ma Bo,Chen Guoguang,Tzeng Chimeng
Liver fibrosis is an aberrant wound healing response to tissue injury characterized by excessive extracellular matrix deposition and loss of normal liver architecture. Hepatic stellate cells (HSCs) activation is regards to be the major process in liver fibrogenesis which is dynamic and reversible. Both Hippo signaling core factor Yap and Hedgehog (Hh) signaling promote HSCs transdifferentiation thereby regulating the repair process of liver injury. However, the molecular function of YAP and the regulation between Yap and Hh during fibrogenesis remain uncertain. In this study, the essential roles of Yap in liver fibrosis were investigated. Yap was detected to be increased in liver fibrotic tissue by the thioacetamide (TAA)-induced zebrafish embryonic and adult models. Inhibition of Yap by both embryonic morpholino interference and adult’s inhibitor treatment was proved to alleviate TAA-induced liver lesions by and histology and gene expression examination. Transcriptomic analysis and gene expression detection showed that Yap and Hh signaling pathway have a cross talking upon TAA-induced liver fibrosis. In addition, TAA induction promoted the nuclear colocalization of YAP and Hh signaling factor GLI2α. This study demonstrates that Yap and Hh play synergistic protective roles in liver fibrotic response and provides new theoretical insight concerning the mechanisms of fibrosis progression. Graphical abstract
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高影响因子
JOURNAL OF BIOMEDICAL SCIENCE
·
2023
·Vol. 30
·期 1
·1-19
·
IF 11
Li Peisi,Zhou Dawang,Chen Dongwen,Cheng Yikan,Chen Yuan,Lin Zhensen,Zhang Xi,Huang Zhihong,Cai Jiawei,Huang Wenfeng,Lin Yanyun,Ke Haoxian,Long Jiahui,Zou Yifeng,Ye Shubiao,Lan Ping
Background A large proportion of the patients with cancer do not respond to immunotherapies. Recent studies suggested an important role for tumor-infiltrating cytotoxic T lymphocytes (CTL) in enhancing response to immunotherapy. Here, we aim to identify gene that induce proliferative and cytotoxic states of CD8 + T cells, and to investigate its effect on CAR-T cells against colorectal cancer. Methods Correlation between the expression of IFI35 with the activation and cytotoxicity of CD8 + T cells was assessed with TCGA and proteomic databases. Then we constructed murine colon cancer cells over-expressing IFI35 and tested their effect on anti-tumor immunity in both immunodeficient and immunocompetent mouse models. Flow cytometry and immunohistochemistry were performed to assess the immune microenvironment. Western blot analysis was used to identify the potential down-stream signaling pathway regulated by IFI35. We further investigated the efficacy of the rhIFI35 protein in combination with immunotherapeutic treatment. Results The transcriptional and proteomic analysis of the activation and cytotoxicity of CD8 + T cells in human cancer samples demonstrated that IFI35 expression is correlated with increased CD8 + T cell infiltration and predicted a better outcome in colorectal cancer. The number and cytotoxicity of CD8 + T cells were significantly increased in IFI35-overexpressing tumors. Mechanistically, we identified that the IFNγ-STAT1-IRF7 axis stimulated IFI35 expression, and that IFI35-mediated regulation of CD8 + T cell proliferation and cytotoxicity was dependent on PI3K/AKT/mTOR signaling pathway in vitro. Furthermore, IFI35 protein enhanced the efficacy of CAR-T cells against colorectal cancer cells. Conclusion Our findings identify IFI35 as a new biomarker that can enhance the proliferation and function of CD8 + T cells, as well as increase the efficacy of CAR-T cells against colorectal cancer cells. Graphical Abstract
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JOURNAL OF NANOPARTICLE RESEARCH
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2023
·Vol. 25
·期 7
·1-16
·
IF 2.5
Shan Chuanfu,Liu Ziyi,Li Fuchao,Jia Wenhan,Cai Gaotuo,Li Ming,Tang Tao,Li Shuai,Wen Jianfeng,Hu Guanghui,Jiang Li,Li Xinyu
TiO 2 -based materials are important photocatalysts but the rapid recombination of photoexcited electron–hole pairs, and the wide band gap both limit their photocatalytic activities. In this work, p-type phosphorus-doped graphene quantum dots (P-GQDs, 5~20 nm) were combined with n-type TiO 2 nanoparticles (20~50 nm) and n-type silver iodide (AgI, 100~200 nm) by a facile sonication and precipitation process to yield a novel ternary photocatalyst TiO 2 /P-GQDs/AgI with n-p-n heterojunctions. The morphologies, structures, optical properties, and chemical environments of photocatalysts were all studied. The resulting ternary photocatalyst exhibited enhanced visible photocatalytic activity toward the degradation of methyl orange (MO), estimated to 70.4-fold that of pure TiO 2 . By contrast, the photocatalytic activities of TiO 2 /GQDs with an n-n heterojunction, TiO 2 /P-GQDs with a p-n heterojunction, and TiO 2 /GQDs/AgI (30%) with an n-n-n heterojunction showed 4.4-fold, 5.3-fold, and 14.2-fold higher than those of TiO 2. The electrochemical and optical measurements revealed that the enhanced photocatalytic activity was linked to promoted charge carrier separation by n-p-n heterojunctions coupled with the increased absorption in the visible region. Overall, the new band structure connected in series p-n heterojunctions looks promising as a highly efficient photodegradation system for future use in decontamination of polluted water. Graphical abstract
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CELLULOSE
·
2023
·Vol. 30
·期 10
·6695-6718
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IF 5.7
Zhang Xuefeng,Li Dan,You Fei,Li Gang,Zhou Chang,Cheng Dan,Pan Yu,Wang Junqi,Ma Jing
Cotton fabrics were treated with Gelatin (GEL)/Adenosine 5′-monophosphate (AMP) by layer-by-layer (LBL) assembly coatings and SiO 2 sol–gel coatings to improve flame retardancy. Functional groups, surface element compositions, crystal structures, surface microscopic morphologies and element distributions of original and treated cotton fabrics were characterized by techniques including Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and Scanning electron microscopy–Energy dispersive spectrometer (SEM–EDX). The results of FTIR, XPS, XRD and SEM–EDX show that the composite coatings are successfully deposited onto cotton fiber surfaces. Thermogravimetric, limiting oxygen index (LOI) experiments, vertical flammability tests (VFTs) and cone calorimetry tests (CCTs) were used to assess the thermal stabilities, flame retardancy levels and reaction-to-fire properties of cotton fabrics. The washing durability, air permeability and hydrophobicity levels were investigated by American Association of Textile Chemists and Colorists, Chinese national standard and water contact angle tests. Results show that the 10BL-SiO 2 @COT exhibits optimum flame retardancy level. Its char residue rate at 700.0 ℃ increases by 506.7% when comparing with original cotton fabric. It has a LOI value of 30.3% (non-flammable level). It can self-extinguish with a char length of only 4.4 cm during a VFT. Its total heat release and peak heat release rate reduce by 18.7% and 50.4% when comparing with original cotton fabric. Its fire growth rate value decreases to 2.2 kW/(m 2 ·s) with a synergistic effectiveness parameter of 1.3 (≥ 1.0). In addition, its LOI value decreases by 26.1% after 10 laundering cycles, but its air permeability enhances by 5.8% as compared with original cotton fabric. Also, it is very hydrophilic. GEL/AMP LBL assembly and SiO 2 gel coatings play more synergistic roles in providing physical barriers and structural supports, promoting and reinforcing char formations, cooling burning zones, diluting oxygen and gaseous products.
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JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 17
·7319-7332
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IF 4.5
Hou Zhaoping,Tian Zihan,Zhao Junming,Yang Xinhe,Liu Liangliang
Zeolite imidazole framework-8 (ZIF-8), serving as a promising adsorbent for wastewater treatment, is limited in practical wastewater treatment due to its polycrystalline powder form resulting in poor recoverability. Herein, the millimeter-scale ZIF-8@porous mullite beads were prepared by the seeded-secondary growth method. The surface of the porous mullite beads is fully encapsulated by nanoscale ZIF-8 crystals, and the ZIF-8 crystals are anchored by mullite whiskers. This composition provides ZIF-8 with a stable macroscopic structure and maintains the exposure of its adsorption sites. Therefore, the ZIF-8@porous mullite beads exhibited good adsorption performance toward methyl orange (MO) and orange G (OG) in the aqueous solution. The analysis of adsorption kinetics indicating the adsorption efficiency of ZIF-8@mullite-w on MO and OG is influenced by the concentration of adsorbates and the adsorption sites. The maximum adsorption of ZIF-8@porous mullite beads on MO and OG was 16.5289 and 5.111 mg/g, respectively.
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EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2023
·Vol. 249
·期 7
·1875-1885
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IF 3.3
Lu Yuanchao,Hou Rongrong,Li Mengren,Yu Ningxiang,Huan Weiwei,Nie Xiaohua,Meng Xianghe
Camellia oleifera Abel is a traditional and high-quality oil source in China, characterized as polyphenols. This study evaluated how extraction methods impacted the phenolic profiles and antioxidant activities of Camellia oils. The contribution rates of phenolic profiles to antioxidant activities were investigated. HPLC and UPLC-Triple-TOF/MS demonstrated that phenolic content significantly varied with extraction methods while phenolic profiles were not. Herein, 15, 15, 17, 14, and 14 compounds were identified in mechanical pressing (MP), solvent extraction-hexane (SE-H), solvent extraction-ethanol (SE-E), aqueous enzymatic extraction (AEE), aqueous extraction (AE) oils, respectively. Results presented that SE-E oils were rich in phenolic acids (caffeic acid and p-hydroxybenzaldehyde), while AE oils were abundant in flavonoids (rutin and quercetin). Besides, the antioxidant activities of AEE and AE oils were significantly higher than other oils, originating from flavonoids (rutin and quercetin) and non-phenolics (rhododendron acid). Furthermore, Camellia oils from five extraction methods were remarkably classified by principal component analysis based on phenolic categories and antioxidant activities. It was assumed that low-polar and high-polar phenolic profiles were simultaneously transferred into SE-E oils, while only low-polar phenolic profiles (mainly flavonoids) were extracted into AE or AEE oils. The antioxidative origin of Camellia oils was ascribed to rutin, quercetin, and rhododendron acid. Our results provided an in-depth understanding of Camellia oils on phenolic profiles and the relationship between extraction methods and antioxidant activities.
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Nano Research
·
2023
·Vol. 16
·期 7
·10131-10138
·
IF 9.9
Wang Yawen,Gao Heng,Liu Yuanlan,Li Dong,Zhao Bo,Liang Wenkai,Sun Yinghui,Jiang Lin
Al nanoparticles (NPs) exhibit excellent localized surface plasmon resonance (LSPR) properties and have been considered a promising alternative to plasmonic Au or Ag NPs. However, it remains difficult to fabricate Al NPs with uniform size and controllable morphology over a large area on substrates, which seriously hinders the in-depth exploration of their properties and applications. Herein, we have developed a self-assembly nanoparticle template method to realize the controllable preparation of bowl-shaped Al NPs (Al nanobowls (Al NBs)) with tunable sizes from 36 to 131 nm on the substrate surface, accompanied by tunable LSPR spectral responses from 272 to 480 nm. Among them, 131 nm Al NBs exhibit superior fluorescence enhancement ability (1932.2-fold) and a low detection limit (78.6 pM) towards 5-carboxyfluorescein, exceeding comparable Ag NBs and Au nanospheres (NSs). This can be attributed to the strong electromagnetic enhancement induced by the LSPR effect and the effective inhibition of fluorescence quenching caused by the self-passivated oxide layer. Therefore, the successful fabrication of Al NBs on substrates is of vital significance for their promising applications, including surface-enhanced spectroscopy, sensitive fluorescence detection, light-harvesting devices, biosensing, and ultraviolet (UV) plasmonics.
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-12
·
IF 16.6
Ba Ziyu,Luo Hongyun,Guan Juan,Luo Jun,Gao Jiajia,Wu Sujun,Ritchie Robert O.
High-performance, fast-growing natural materials with sustainable and functional features currently arouse significant attention. Here, facile processing, involving delignification, in situ hydrothermal synthesis of TiO 2 and pressure densification, is employed to transform natural bamboo into a high-performance structural material. The resulting TiO 2 -decorated densified bamboo exhibits high flexural strength and elastic stiffness, with both properties more than double that of natural bamboo. Real-time acoustic emission reveals the key role of the TiO 2 nanoparticles in enhancing the flexural properties. The introduction of nanoscale TiO 2 is found to markedly increase the degree of oxidation and the formation of hydrogen bonds in bamboo materials, leading to extensive interfacial failure between the microfibers, a micro-fibrillation process that results in substantial energy consumption and high fracture resistance. This work furthers the strategy of the synthetic reinforcement of fast-growing natural materials, which could lead to the expanded applications of sustainable materials for high-performance structural applications.
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Nano Research
·
2023
·Vol. 16
·期 5
·6568-6576
·
IF 9.9
Zhang Genrui,Li Xiaojing,Li Na,Wu Tingting,Wang Lei
Constructing 2D/2D face-to-face heterojunctions is believed to be an effective strategy to enhance photocatalytic performance due to the enlarged contact interface and increased surface active sites. Herein, 2D porous NiCo oxyphosphide (NiCoOP) was synthesized for the first time and coupled with graphitic carbon nitride (g-C 3 N 4 ) nanosheets to form 2D/2D heterojunctions via an in-situ phosphating method. The optimal 4 wt.% 2D/2D NiCoOP/g-C 3 N 4 (OPCN) photocatalyst achieves a hydrogen evolution rate of 1.4 mmol·h −1 ·g −1 , which is 33 times higher than that of pure g-C 3 N 4 . The greatly improved photocatalytic performance of the composite photocatalysts could be attributed to the formation of interfacial surface bonding states and sufficient charge transfer channels for accelerating carrier separation and transfer and the porous structure of NiCoOP nanosheets with abundant surface active sites for promoting surface reactions. Amazingly, the 2D/2D OPCN composite photocatalysts also exhibit superior stability during photocatalytic reactions. This study not only designs new noble-metal-free NiCoOP/g-C 3 N 4 composite photocatalysts but also provides a new sight in fabricating face-to-face 2D/2D heterojunctions for their application in energy conversion areas.
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BMC NEUROSCIENCE
·
2023
·Vol. 24
·期 1
·1-12
·
IF 2.4
Cai Jimin,Yang Yang,Han Jiahui,Gao Yu,Li Xin,Ge Xin
Background Microglial polarization and the subsequent neuroinflammatory response and oxidative stress are contributing factors for traumatic brain injury (TBI) plus hemorrhagic shock (HS) induced brain injury. In the present work, we have explored whether Lysine (K)-specific demethylase 4 A (KDM4A) modulates microglia M1 polarization in the TBI and HS mice. Results Male C57BL/6J mice were used to investigate the microglia polarization in the TBI + HS model in vivo. Lipopolysaccharide (LPS)-induced BV2 cells were used to examine the mechanism of KDM4A in regulating microglia polarization in vitro. We found that TBI + HS resulted in neuronal loss and microglia M1 polarization in vivo, reflected by the increased level of Iba1, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, malondialdehyde (MDA) and the decreased level of reduced glutathione (GSH). Additionally, KDM4A was upregulated in response to TBI + HS and microglia were among the cell types showing the increased level of KDM4A. Similar to the results in vivo, KDM4A also highly expressed in LPS-induced BV2 cells. LPS-induced BV2 cells exhibited enhanced microglia M1 polarization, and enhanced level of pro-inflammatory cytokines, oxidative stress and reactive oxygen species (ROS), while this enhancement was abolished by the suppression of KDM4A. Conclusion Accordingly, our findings indicated that KDM4A was upregulated in response to TBI + HS and microglia were among the cell types showing the increased level of KDM4A. The important role of KDM4A in TBI + HS-induced inflammatory response and oxidative stress was at least partially realized through regulating microglia M1 polarization.
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FIBERS AND POLYMERS
·
2023
·Vol. 24
·期 4
·1265-1274
·
IF 2.5
Yuan Yun,Bao Xueming,Wu Leilei,Wang Ping,Yu Yuanyuan,Wang Qiang
Due to the weak interaction between dye molecule and silk fiber, traditional acid dyeing of silk fabrics has some shortcomings such as high energy consumption and poor dyeing fastness. In the present work, in situ coloring of silk fabric was carried out via graft polymerization of caffeic acid (CA) by using a Fenton system consisting of FeCl 2 and H 2 O 2 . Subsequently, silver ions were reduced to silver nanoparticles (AgNPs) by poly(caffeic acid) (PCA) grafted in silk fibroin and deposited on the fiber surfaces, aiming at improving the antibacterial property of silk fabric. The results indicate that it is feasible to achieve in situ dyeing of silk fabrics under low temperature condition through graft polymerization of CA, and the color depth (K/S value) increases to 4.8. Meanwhile, the iron ions might further form coordination bonds with PCA and tyrosine residues in silk fibers, which can improve the washable resistance and wet rubbing fastness of the dyed fabrics, reaching the grade of 4–5. In addition, PAC grafted on silk fibers exhibits high reductivity, which can reduce silver ions and guide the AgNP deposition on the surface of silk fibers. This allows silk fabrics to achieve efficient and durable antibacterial effects, and the antibacterial rate remains above 98% even after cyclic washings. The present work provides a novel approach for in situ coloring and functionalization of silk fabrics without additional synthetic dyes or antibacterial agents.
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-12
·
IF 16.6
Wang Shuo,Zhao Qian,Hazarika Abhijit,Li Simiao,Wu Yue,Zhai Yaxin,Chen Xihan,Luther Joseph M.,Li Guoran
A detailed picture of temperature dependent behavior of Cs x FA 1-x PbI 3 perovskite quantum dots across the composition range is constructed by performing in situ optical spectroscopic and structural measurements, supported by theoretical calculations that focus on the relation between A-site chemical composition and surface ligand binding. The thermal degradation mechanism depends not only on the exact chemical composition, but also on the ligand binding energy. The thermal degradation of Cs-rich perovskite quantum dots is induced by a phase transition from black γ-phase to yellow δ-phase, while FA-rich perovskite quantum dots with higher ligand binding energy directly decompose into PbI 2 . Quantum dot growth to form large bulk size grain is observed for all Cs x FA 1-x PbI 3 perovskite quantum dots at elevated temperatures. In addition, FA-rich quantum dots possess stronger electron−longitudinal optical phonon coupling, suggesting that photogenerated excitons in FA-rich quantum dots have higher probability to be dissociated by phonon scattering compared to Cs-rich quantum dots.
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高影响因子
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