COLLOIDS AND SURFACES B-BIOINTERFACES
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2015
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IF 4.152
Jianwei Du,Ce Tian,Yajie Liu,Jun Ling,Youxiang Wang
Polypeptide has been extensively researched in gene/drug delivery system due to the good biocompatibility. Herein, we synthesized total-polypeptide copolymers, i.e. Azo(azobenzene)-capped polysarcosine- b -polylysine (ASL) with narrow molecular weight distribution by α-amino acid N-carboxyanhydride (NCA) polymerization. Although the molecular weight of PLL segment was only about 6 kDa, ASL could condense DNA effectively and form about 150 nm spherical nanoparticles at N/P ratio of 15. The surface charge was significantly reduced due to the shielding effect of polysarcosine (PSAR) shell. ASL/DNA PeptoPlexes showed good colloidal stability under physiological salt condition and complexation competition stability in the presence of counter polyanion, which might improve the circulation time in vivo . The tip design of azobenzene provided a facile way for ligand modification via host–guest interaction, which could be flexibly optimized by changing its functional tags responding to a request. Our data showed that the introduction of CD-R8 could promote the internalization of gene into cytoplasm and even nucleus owing to the membrane penetrating effect of R8. Cell culture experiments indicated as a total-polypeptide system, ASL showed good cellular viability and comparable gene transfection level as PLL with molecular weight of 50 kDa. Overall, PSAR served as an ideal alternative of PEG and this total-polypeptide system showed us a good direction for gene carrier design.
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JOURNAL OF LUMINESCENCE
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2024
·
IF 3.3
Yaxin Li,Mei Chen,Hongzhi Lu,Shoufang Xu
A straightforward and highly sensitive ratiometric fluorescence sensor comprising blue emissive carbon dots , silver nanoprisms, and o-phenylenediamine (B-CDs-Ag NPRs-OPD) was developed for the detection of H 2 O 2 and glucose. Ag NPRs were etched into Ag + ions upon exposure to H 2 O 2 , which caused non-fluorescent OPD to be oxidized and produce 2,3-diaminophenazine (DAP), resulting in intense yellow fluorescence emission at 555 nm. DAP can quench the B-CDs based on inner filter effect (IFE), leading to a decrease in their fluorescence intensity at 435 nm. Because glucose oxidase may catalyze the oxidation of glucose to produce H 2 O 2 , this approach can also be used to detect glucose. The unique triangular structure leads to the easy etching of the edge and tip of Ag NPRs by H 2 O 2 into Ag + ions, allowing for the high sensitivity of detection of H 2 O 2 and glucose with limit of detection (LOD) of 0.12 μM and 0.6 μM, respectively. Furthermore, with the increase of the concentrations of H 2 O 2 and glucose, a visible color shift from blue to yellow-green occurs, which can be detected using a smartphone's color recognition application that captures the RGB channel values of the image. Real-time and on-spot detection of H 2 O 2 and glucose were achieved based on smartphone sensing platform, presenting vast potential for applications in various fields.
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CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Haoyu Ma,Jiaozhu Wu,Chengzhe Gao,Silin He,Pengjian Gong,Qiwu Shi,Zhao Wang,Guangxian Li,Chul B. Park
In the context of the future sixth-generation (6G) communication system, the development of multi-functional materials with ultra-low dielectric permittivity ( D k ) and ultra-low dielectric loss ( D f ) is of paramount importance for the design of terahertz (THz) frequency electromagnetic wave transmission devices. In this work, polyimide- block -polydimethylsiloxane (PI- b -PDMS copolymer, PIC) material with low D k , high flexibility and high supercritical fluid foaming ability was fabricated via copolymerization. Additionally, core–shell SiO 2 @BN nanofillers were synthesized by condensing tetraethyl orthosilicate (TEOS) on hexagonal boron nitride (BN) nanofiller surface. Subsequently, flexible polyimide/SiO 2 @BN foam was fabricated via supercritical N 2 foaming. The resulting PIC/SiO 2 @BN nanocomposites exhibit exceptional flexibility and impressive thermal resistance (thermal decomposition temperature 472.2 °C). Notably, the incorporation of core–shell SiO 2 @BN nanofillers leads to the formation of a molecular interface anchoring structure through hydrogen bonding effect. This structure effectively reduced the mobility of the flexible PIC polymer chains on the SiO 2 @BN nanofiller surfaces. Consequently, the porous PIC/SiO 2 @BN materials show a low shrinkage ratio, low dielectric property ( D k = 1.21, D f = 0.012 at 1 THz), excellent THz transmission rate (91.4 %) and long distance 6G THz signal coverage (125.99 m). Furthermore, porous PIC/SiO 2 @BN materials show superior flame-retardant and thermal infrared stealth properties.
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PHYTOTHERAPY RESEARCH
·
2025
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IF 6.3
Qian Hu,Mengyao Wang,Meng Chen,Jinjin Wang,Ting Niu
Diffuse large B-cell lymphoma (DLBCL) is an aggressive and heterogeneous subtype of non-Hodgkin lymphoma, with two-thirds of patients relapsing or resisting existing therapies, highlighting the urgent need for effective treatments. Toosendanin (TSN), a triterpenoid from Meliae Cortex, exhibits significant anti-cancer activity by modulating cell survival and proliferation. This study investigates the anti-lymphoma effects and underlying mechanisms of TSN, proposing it as a potential therapeutic agent to address the challenges of DLBCL. Network pharmacology, molecular docking, and transcriptome sequencing were employed to predict TSN's anti-DLBCL potential. Findings were validated through in vitro and in vivo experiments, including cell viability assays, flow cytometry, quantitative PCR, Western blotting, reverse experiments with small-molecule inhibitors or genetic editing, and a cell-derived xenograft (CDX) model. Bioinformatics analyses revealed TSN's strong binding affinity to PI3Kα/β and Polo-like kinase 1 (PLK1). Experiments showed that TSN downregulated the PI3K/Akt signaling pathway and reduced PLK1 mRNA and protein levels, inducing apoptosis, cell cycle arrest, and cell death in DLBCL cells. RNA sequencing and metabolic assays indicated TSN upregulated cholesterol biosynthesis in DLBCL cells. Co-treatment with a statin enhanced TSN's anti-DLBCL effects while mitigating hepatic and pulmonary toxicity. This study identifies TSN as a dual inhibitor of PI3K and PLK1 with significant therapeutic potential for DLBCL. It also proposes a lipid-modulating strategy to enhance TSN's cytotoxicity while reducing adverse effects, offering a promising approach to improve DLBCL treatment outcomes.
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CLINICAL AND EXPERIMENTAL ALLERGY
·
2025
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IF 5.2
Yanran Huang,Zhaofeng Xu,Yan Yan,Chunbo Li,Jieying Yan,Yi Wei,Jian Li,Luo Zhang,Nan Zhang,Claus Bachert,Weiping Wen
Background Immunoglobulin overproduction was observed in type 2 chronic rhinosinusitis with nasal polyps (CRSwNP). Endoplasmic reticulum (ER) stress is linked to aggregation in various inflammatory diseases, yet its presence and extent in nasal polyps remain to be elucidated. Objective To assess the impact of B-cell ER stress on local immunoglobulin production in CRSwNP and investigate its correlation with disease severity. Methods Single-cell mapped transcriptional profiles at cellular resolution. Electron microscopy revealed ultrastructural features, complemented by immunohistochemistry/immunofluorescence mapping marker localisation. Western blotting quantified protein expression, with QuantiGene Plex and Luminex enabling multiplex cytokine analysis. Bulk RNA sequencing and targeted protein expression validation in ex vivo experiments confirmed critical findings. Results HSPA5 and HSP90B1 were found to be two major elevated ER stress markers in type 2 CRSwNP, compared to the control nasal tissue, and their expression correlated with the expression of IGHE and type 2 inflammatory markers. In CRSwNP, the ER stress signature score and increased marker expression predominantly originated from B and plasma cells. Electron microscopy revealed dilated ER and enlarged lumen in sorted B cells from nasal polyps. MZB1 exhibited co-localisation with plasma cells and mature B cells. Immunofluorescence staining demonstrated that MZB1 co-localised with HSPA5 and HSP90B1. In vitro, stimulation with MZB1 upregulated mRNA expression of ER stress markers and IgE. Increased IGHE expression was detected in response to ER stress induced in vitro. Finally, anti-IgE treatment inhibited the expression of ER stress-related genes. Conclusion ER stress markers were significantly upregulated in CRSwNP. Specifically, ER stress levels were significantly elevated in B cells of CRSwNP compared to controls. In type 2 CRSwNP, B-cell ER stress may play a role in promoting local IgE production. Graphical The ER stress burden was significantly upregulated in type 2 CRSwNP tissues compared to both healthy controls and non-type 2 polyp tissues. This elevated stress was linked to more severe type 2 inflammation and increased local IgE production. In vitro studies of B cells derived from nasal polyp tissues demonstrated that MZB1 upregulated endoplasmic reticulum stress, which was associated with enhanced IgE level. Furthermore, in vivo treatment with Omalizumab reduced endoplasmic reticulum stress levels. ER, endoplasmic reticulum; HC, healthy controls; CRSwNP, chronic rhinosinusitis with nasal polyps; MZB1, Marginal Zone B1 protein.
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ANALYTICA CHIMICA ACTA
·
2024
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IF 5.7
Hang Gong,Ganping Cai,Yong Li,Ning Jiang,Chunyan Chen,Feng Chen,Changqun Cai
Background Viral epidemics have long endangered human health and had dramatic impacts on environment and society. The currently known viruses and the rapid emergence of previously unknown viruses lead to an urgent need for effective virus detection strategies. It is important to develop methods that can detect multiple related viruses simultaneously in order to improve detection efficiency and to avoid treatment delays due to misdiagnoses. However, an important practical problem for viruses simultaneously detection is mutual interference between the targets, which is caused by a certain degree of signal superposition. Results A portable ratio-based triple-emission molecular imprinting fluorescence sensor was constructed to detect hepatitis A virus (HAV) and hepatitis B virus (HBV) simultaneously, which eliminated background interference and mutual interference between the two targets, and improved detection accuracy. In this sensor, a layer containing blue fluorescent carbon quantum dots (B-CDs) coated with silicon was used as a constant luminescent core to permit ratiometric detection of signals, this layer also affords visual analysis due to its constant blue color. As signal sources to employ yellow-green and red quantum dots, HAV- and HBV-specific molecularly imprinted polymers (MIPs) were prepared by imprinting virus onto the core containing the B-CDs. Under the optimized detection conditions, the simply physically mixing HAV-MIPs and HBV-MIPs in a specific ratio, which leads to non-interfering fluorescence signals and the simultaneous detection of the two target viruses via the rich color visualization under ultraviolet light. Significance This strategy provides a simple and low-cost (0.26 ¥/sensor) method for the simultaneous detection of two hepatitis viruses, and provides an avenue for the development of portable methods for the simultaneous detection of similar highly infectious viruses. This self-service sensor also has the potential to be used by patients themselves, reducing pressures on medical staff and decreasing risks of cross-infection.
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RSC Advances
·
2014
·Vol. 4
·期 78
·41588-41596
·
IF 3.708
Lixin Yang,Xiuli Hu,Weiqi Wang,Shi Liu,Tingting Sun,Yubin Huang,Xiabin Jing,Zhigang Xie
Y-shaped amphiphilic block copolymers, (methoxy-poly(ethylene glycol))2-block-poly(L-glutamic acid) ((mPEG)2-PGA) and its precursor (methoxy-poly(ethylene glycol)2-block-poly(γ-benzyl-L-glutamate) ((mPEG)2-PBG), were prepared in three steps: (1) macroinitiator (methoxy-poly(ethylene glycol))2-NH2 ((mPEG)2-NH2) was synthesized by coupling two methoxy-poly(ethylene glycol)s with serinol and diisocyanate. (2) (mPEG)2-PBG was synthesized by ring opening polymerization of γ-benzyl-L-glutamate-N-carboxyanhydride initiated with the macroinitiator ((mPEG)2-NH2); (3) the protective benzyl groups in (mPEG)2-PBG were removed to obtain (mPEG)2-PGA. The properties of both (mPEG)2-PBG and (mPEG)2-PGA were characterized by 1H NMR, FT-IR, GPC, and DLS. In aqueous solution (mPEG)2-PBG tends to form more stable micelles compared to linear mPEG-PBG copolymer. The size of (mPEG)2-PBG decreases with increasing length of hydrophobic PBG in (mPEG)2-PBG. Paclitaxel and cisplatin were grafted onto (mPEG)2-PGA to form (mPEG)2-PGA-PTX (MPTX) with a grafting ratio of near 90% and (mPEG)2-PGA-Pt (MPt) conjugates with a loading efficacy of 15% (w/w). MPTX can greatly improve the solubility of PTX. Both conjugates can self-assemble into micelles with a mean diameter of about 50 nm and show enhanced anti-cancer activity against MCF-7, HeLa, and SMMC cell lines. The in vivo anticancer evaluation in mice shows MPt showed a desirable antitumor activity and allowed us to deduce the system toxicity. Therefore, both MPTX and MPt have a great potential as a polymer drug in cancer chemotherapy.
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CATALYSIS LETTERS
·
2022
·Vol. 152
·期 12
·3594-3606
·
IF 2.936
Yao Haoyang,Xu Yunlan,Zhong Dengjie,Zeng Yundong,Zhong Nianbing
In order to inhibit rapid recombination of electron–hole pairs of photoanode and expand its light response wavelength, PPy-14%/WO 3 /TiO 2 /Ti photoanode was prepared. The maximum photocurrent density is 2.31 mA·cm −2 , which is 1.76 times higher than that of WO 3 /TiO 2 /Ti at 1.1 V (vs. SCE). The maximum power density, phontocurrent density and Rhodamine B degradation rate of photocatalytic fuel cell (PFC) with PPy-14%/WO 3 /TiO 2 /Ti photoanode and Cu cathode are 25.48 μW·cm −2 , 0.25 mA·cm −2 and 88.5%, respectively. The excellent performance is mainly due to the good carrier migration rate of PPy and the synergistic effect of PPy/WO 3 /TiO 2 /Ti ternary hybrid electrode, so that photogenerated electrons and holes can accumulate in the conduction band of WO 3 and HOMO of PPy, respectively. This not only expands the light absorption range and enhances the light absorption intensity, but also improves the separation of electron–hole pairs, and finally significantly enhances the Rhodamine B degradation and electricity generation performance of PFC. Graphical Abstract In order to inhibit rapid recombination of electron–hole pairs of photoanode and expand its light response wavelength, PPy-14%/WO 3 /TiO 2 /Ti photoanode was prepared. The excellent performance is mainly due to the good carrier migration rate of PPy and the synergistic effect of PPy/WO 3 /TiO 2 /Ti ternary hybrid electrode, so that photogenerated electrons and holes can accumulate in the conduction band of WO 3 and HOMO of PPy, respectively.
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Materials Today Advances
·
2022
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IF 9.918
Qingdong Li,Jianmin Xiao,Caiyun Zhang,Peng Wang,Wenling Li,Yinfeng Wang,Lang Ran,Xia Qin,Miao Yan,Junchao Qian,Zhengyan Wu,Geng Tian,Pengfei Wei,Guilong Zhang
The development of highly efficient magnetic resonance imaging (MRI) contrast agent contributes to the early and accurate diagnosis of cancer. Herein, we developed a novel iron oxide and gadolinium oxide (IO/GdO) nanocomposite as T 1 -T 2 dual modal MRI contrast agent through hydrothermal synthesis method. Subsequently, tumor-targeting ligand (PDGFB-PEG) was further conjugated to the surface of IO/GdO nanocomposite (PDGFB-pIO/GdO) to fabricate tumor-targeting MRI probe for accurate diagnosis of orthotopic prostatic cancer. IO/GdO nanocomposite exhibited obvious mesoporous structure, which would promote the contact between water molecules and Gd or Fe centers and enhance the T 1 relaxation rate (r 1 ) and T 2 relaxation rate (r 2 ) of IO/GdO composite. In addition, the r 1 and r 2 values of IO/GdO composite were up to be 11.6 mM −1 s −1 and 102.7 mM −1 s −1 , respectively, and the r 2 /r 1 ratio of IO/GdO nanocomposite was calculated to be 8.85, demonstrating a great potential as dual-mode MRI contrast agent. Further study indicated that PDGFB-pIO/GdO exhibited an excellent biocompatibility. Besides, PDGFB-pIO/GdO presented a higher accumulation in tumor than untargeted pIO/GdO, confirming its excellent in vivo tumor-targeting ability. Lastly, in orthotopic prostatic cancer model, the systemic delivery of PDGFB-pIO/GdO significantly enhanced T 1 - and T 2 -weighted signal of orthotopic prostatic cancer, achieving high quality of MR images and realizing accurate diagnosis of orthotopic prostatic cancer. Collectively, we proposed a rational designed nanoplatform, PDGF-B conjugating mesoporous IO/GdO nanocomposites, suggesting potential for diagnostic identification of prostate cancer via T 1 -T 2 dual-modal MRI contrast enhancement.
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Photodiagnosis and Photodynamic Therapy
·
2023
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IF 3.3
Yuanyuan Liu,Zhiqiang Cao,Guo Wei
Background The incidence of cutaneous squamous cell carcinoma (cSCC) has been demonstrating yearly increases. cSCC is a highly malignant cancer and exerts a major impact on patients' health and quality of life. Thus, the development and use of novel therapies in the treatment of cSCC are urgently needed. It has been reported that LED photodynamic therapy (LED PDT) mediated by Hypocrellin B and its derivatives, a second-generation photosensitizer, can induce apoptosis in a variety of tumor cells, However, its potential pro-apoptotic effects on cSCC have yet to be investigated. Objective This study aims to investigate the pro-apoptotic effects and molecular mechanisms of HB-LED PDT in cutaneous squamous cell carcinoma A431 cells (Subsequent abbreviation A431 cells). Such information can provide an important theoretical foundation for the clinical translation of HB-LED PDT in the treatment of cSCC. Methods 1. Effects of HB on A431 cells were determined using a Cell Counting Kit-8 assay, which method can indirectly reflect the number of living cells. In this way, this assay can then provide a means to identify the optimal concentrations of HB required for the induction of apoptosis in A431 cells. 2. The effects of HB-LED PDT on the morphology of A431 cells and changes in the nuclei after Hoechst33342 staining as determined using inverted fluorescent microscopy. 3. Use of the Annexin V-FITC test kit to detect levels of apoptosis in A431 cells in response to treatment with HB. Changes in reactive oxygen species and mitochondrial membrane potential following HB-LED PDT treatment in A431 cells were determined using fluorescence activated cell sorting (FACS). 4. Real-time quantitative PCR and Western Blot were applied to assess changes in several key factors involved in apoptosis including Bax, Bcl-2, and Caspase-3, at both transcription and translation levels. With these assays, it was possible to investigate the apoptotic signaling pathway in A431 cells in response to HB-LED PDT. Results HB-LED PDT inhibited proliferation activity and promoted nuclear fragmentation within these A431 cells. HB-LED PDT inhibited mitochondrial activity, increased reactive oxygen species production, and promoted apoptosis of A431 cells. In addition, several key factors in the apoptotic signaling pathway were increased at both the transcriptional and translational levels in A431 cells in response to the HB-LED PDT, indicating that the apoptotic signaling pathway was activated by HB-LED PDT. Conclusion HB-LED PDT induces apoptosis in A431 cells through a mitochondria-mediated apoptotic pathway. Such findings serve as an important foundation for the development of new approaches in the treatment of cSCC.
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Translational Cancer Research
·
2026
·Vol. 15
·期 9
·687
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IF 2.1
Zichen Liu,Jiaming Wan,Guoli Deng,Mingzhu Zhang,Shaohua Zhang,Qizhi Liu,Liu Ouyang,Shupeng Liu
Background Hepatitis B virus (HBV) infection is one of the major risk factors for hepatocellular carcinoma (HCC) development. Current prognostic assessment of HBV-associated HCC (HBV-HCC) largely relies on clinicopathological parameters, such as tumor stage, liver function, and serum alpha-fetoprotein (AFP) levels, which have limited accuracy in identifying patients at high risk of recurrence or poor survival. Therefore, reliable molecular biomarkers are urgently needed to improve individualized risk stratification and guide postoperative surveillance and therapeutic decision-making. HBV infection induces chronic inflammation through mechanisms such as genomic integration, ultimately leading to HCC. Cell membrane proteins play critical roles in maintaining cellular homeostasis, signal transduction, and immune recognition, and may be importantly involved in HBV-HCC. However, the prognostic significance of HBV-associated membrane proteins has not been sufficiently investigated. This study aimed to identify HBV-associated membrane proteins through integrated proteomic analysis and to evaluate their prognostic value for recurrence-free survival (RFS) and overall survival (OS) in patients with HBV-HCC. Methods HBV-associated membrane proteins were identified in HepG2 and Hep3B cells using two parallel proteomic approaches: membrane fractionation and Bacillus subtilis (BS2)-based proximity labeling, both coupled with mass spectrometry (MS). These findings were integrated with proteomic analysis of HBV-HCC tissue samples. Prognostic models for RFS and OS were constructed using machine learning algorithms including least absolute shrinkage and selection operator (LASSO)-Cox (proportional hazards model), random survival forest (RSF), and elastic net (EN). Results A total of 98 HBV-associated membrane proteins were identified, including canonical membrane proteins as well as several nuclear and cytoplasmic proteins. Functional annotation revealed numerous tumor-associated molecules, transporters, and enzymes. Univariate and multivariate analyses identified proteins such as POLM and TMEM192 as prognostic factors for RFS, and GLYATL1 and TMEM109 for OS. The prognostic models developed using machine learning achieved receiver operating characteristic (ROC) values exceeding 0.7. Conclusions This study identified HBV-HCC-related membrane proteins beyond classical localized molecules using membrane fractionation and BS2 proximity labeling, and established robust prognostic models through multiple machine learning approaches. These findings provide potential therapeutic targets and prognostic biomarkers that may improve the clinical management of HBV-HCC patients. However, the single-cohort design, absence of external validation, and potential model optimism preclude claims of clinical robustness or immediate application. Independent multicenter validation and standardized targeted protein assays are required before these proteins or prediction models can be considered for clinical risk stratification.
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RSC Advances
·
2018
·Vol. 8
·期 22
·12459-12470
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IF 2.936
Minghua Wang,Longyu Yang,Jinyun Yuan,Linghao He,Yingpan Song,Hongzhong Zhang,Zhihong Zhang,Shaoming Fang
A series of bismuth sulfide (Bi2S3) nanorods and amine-functionalized Ti-based metal–organic framework heterojunctions [denoted by Bi2S3@NH2-MIL-125(Ti)] were constructed and explored as bifunctional photocatalysts for Cr(VI) reduction and rhodamine B (RhB) degradation under visible light illumination. Compared with the individual NH2-MIL-125(Ti) and Bi2S3, the as-synthesized Bi2S3@NH2-MIL-125(Ti) photocatalyst exhibited an enhanced photocatalytic activity toward Cr(VI) and RhB owning to the synergetic effect between Bi2S3 and NH2-MIL-125(Ti). Moreover, the Bi2S3@NH2-MIL-125(Ti) heterojunctions showed increased Cr(VI) removal efficiency by adding RhB in the system. The photocatalytic mechanism was proposed based on the analysis of different scavenger for active species and electron spin resonance spectrometry. The introduction of Bi2S3 into NH2-MIL-125(Ti) can extend the light adsorption and improve the transfer and separation of photogenerated charge carriers through the Bi2S3@NH2-MIL-125(Ti) heterojunction with unique band gap structure. The synthesized Bi2S3@NH2-MIL-125(Ti) photocatalyst also exhibited good reusability and stability.
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