CATALYSIS LETTERS
·
2018
·Vol. 148
·期 6
·1723-1730
·
IF 2.911
He Chuan,Zheng Shanshan,Zhang Jinli,Li Wei,Fu Yan
Abstract Guanine-rich DNA strands have the potential to activate Cu 2+ ions in peroxidase-like reactions based on the coordination of Cu 2+ with nucleobases. Comparative studies on the number of guanines elucidate a sequence dependency that contiguous guanines are crucial to intrinsic peroxidase mimicking activities of DNA-Cu(II). The optimal number of repeat units to boost the enzyme-like activity of Cu 2+ is identified via analyzing individual sequence of (T 3 G 2 ) n and (T 2 G 3 ) n . The catalytic performance of DNA-Cu(II) was found to be remarkably pH-tolerant through the H 2 O 2 -mediated oxidation of 3,3′,5,5′-tetramethylbenzidine at acidic pH, dopamine at neutral pH as well as methylene blue at alkaline pH, respectively. It is the first time to report that intrinsic peroxidase-like activities of DNA-Cu(II) are greatly associated with binding affinity and stoichiometry between Cu 2+ and DNA. (T 3 G 2 ) 4 -Cu(II) with a dominant 1:1 stoichiometry shows the K
m
toward H 2 O 2 of 93 mM at pH 4.0 and 8.65 mM at pH 11.0. This study paves a way to rationally design artificial metalloenzymes, and the ability to tune the enzymatic activity would be harnessed for biocatalysis, biosensing, green synthesis and nanoscience. Graphical Abstract Remarkable pH-tolerant peroxidase mimetics was constructed by direct coordination of Cu 2+ within a guanine-rich DNA scaffold, of which the enzymatic activity is greatly associated with binding affinity and stoichiometry between Cu 2+ and DNA. Intriguingly, there exist optimal guanine numbers and strand lengths to boost the catalytic performance of Cu 2+ .
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 6
·1-10
·
IF 5.479
Luo Zibin,Sun Duanping,Tong Yanli,Zhong Yongsheng,Chen Zuanguang
An ultrasensitive voltammetric aptasensor was constructed to analyze cardiac troponin I (cTnI). It is based on DNA nanotetrahedron (NTH) linked dual-aptamer (dAPT) and magnetic metal organic frameworks (mMOFs) of type Fe 3 O 4 @UiO-66. Firstly, the DNA NTH linked dAPT (Tro4 and Tro6) were immobilized on a gold electrode for improving the capture efficiency of cTnI. The novel mMOFs Fe 3 O 4 @UiO-66 was then decorated by Au@Pt nanoparticles (Au@PtNPs), horseradish peroxidase (HRP), G-quadruplex/hemin (GQH) DNAzyme, and two types of aptamers to form signaling nanoprobes. In the presence of cTnI, an aptamer-protein-nanoprobe sandwich-type structure is formed. Afterward, the nanoprobes including enzyme, GQH DNAzyme and Fe 3 O 4 @UiO-66/Au@PtNP were utilized to catalyze the oxidation of hydroquinone by hydrogen peroxide for the electrochemical signals amplification, typically at a working potential of −0.1 V (vs. Ag/AgCl). The voltammetric signal increases linearly in the 0.01 to 100 ng·mL −1 cTnI concentration range, and the detection limit is 5.7 pg·mL −1 .
An ultrasensitive voltammetric aptasensor was constructed to analyze cardiac troponin I (cTnI) based on DNA nanotetrahedron linked dual-aptamer and magnetic metal organic frameworks of type Fe 3 O 4 @UiO-66. The results indicated the aptasensor has a wide linear response range (0.01 to 100 ng/mL) and low detection limit (5.74 pg/mL) for cTnI. GE: gold electrode; MCH: 6-Mmercapto-1-hexanol; HRP: horseradish peroxidase; HQ: hydroquinone; BQ: benzoquinone.
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PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2015
·Vol. 25
·期 23
·3541-3550
·
IF 11.805
Bang Lin Li,Hao Lin Zou,Lu Lu,Yu Yang,Jing Lei Lei,Hong Qun Luo,Nian Bing Li
Size-dependent optical absorption of semiconductive (2H) layered molybdenum disulfide (MoS 2 ), exhibiting great discrimination abilities to single- and double-stranded DNA (ssDNA) and (dsDNA), is studied. In the presence of high concentration of salt, layered MoS 2 trends to aggregate rapidly, leading to the increases of sizes in both vertical and lateral dimensions of the nanosheets, which results from the interplay between van der Waals attraction and electrical double-layer repulsion. Meanwhile, the aggregation behavior of layered MoS 2 is remarkably inhibited by the synergistic effects of DNA oligonucleotides. ssDNA can adsorb on the surface of layered MoS 2 , resulting in a great dispersion, even in the presence of high concentration of salt, while the dispersion behavior is weakened when ssDNA is replaced by dsDNA. Whereas compared to graphene with zero bandgap energy, layered MoS 2 , with semiconductive properties, exhibits great characteristic optical absorption in visible wavelength region devoted to exploring the aggregation behavior of layered MoS 2 . Therefore, DNA oligonucleotides induced size control of layered MoS 2 , contributing to the regular change of its characteristic absorption in visible region, is considered a label-free bioassay for the detection of single-nucleotide polymorphism. Due to its easy operation and high specificity, it is expected that the proposed assay holds great promise for further applications.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Xiaoying Wang,Yanqun Shan,Miao Gong,Xin Jin,liangrui Lv,Meng Jiang,Jun Xu
A novel electrochemical sensor for ochratoxin A (OTA) based on the hairpin aptamer (HA) and double report DNA was constructed successfully. In the work, HA was chosen as the OTA recognition element and immobilized on the gold electrode (GE) via Au-S. Au nanoparticles (AuNPs) labeled double report DNA (rDNA1 and rDNA2) were symmetrically hybridized with HA simultaneously. Compared to the single report DNA, the strategy can dramatically increase the amount of AuNPs on the electrode surface and the stability of the HA to prevent lodging. The signal amplification was further implemented by using trithiocyanuric acid (TCA) to aggregate the AuNPs. Thus, it was observed that the differential pulse voltammetry (DPV) response of AuNPs was significantly amplified via multiple signal amplification strategy. When OTA was present, the HA was specifically combined with OTA to form the OTA-HA complex, which caused double report DNA to detach from the electrode, resulting in the decrease of the DPV response. The proposed electrochemical aptasensor can sensitively detect low concentrations of OTA by monitoring the OTA-dependent DPV intensity change. Under the optimized conditions, it exhibited wide linear ranges from 1 pg mL −1 to 1 ng mL −1 with a low detection limit of 0.5 pg mL −1 . The work provides a new idea for the various mycotoxins and even other substances detection at trace level.
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Wenting Wang,Xiaojian Fan,Shenghao Xu,Jason J. Davis,Xiliang Luo
A label-free and low fouling biosensor based on functional polyethylene glycols selective for breast cancer susceptibility gene (BRCA1) is reported. Sensory interfaces were prepared through the modification of a glassy carbon electrode with highly cross-linked polyethylene glycol (PEG) film containing amine groups, followed by the self-assembly of gold nanoparticles and the immobilization of BRCA1 complementary single-strand 19-mer oligonucleotides . In the presence of a specific BRCA1 sequence capture and hybridization results in interfacial change sensitively monitored using electrochemical impedance spectroscopy. The combined utilization of a PEG polymer film and gold nanoparticle mixed interface enables very high levels of sensitivity and a highly effective assaying in patient samples. Assay linear range was from 50.0 fM to 1.0 nM, with a limit of detection of 1.72 fM. Furthermore, this label-free DNA sensor has been used for assaying BRCA1 in serum samples, showing its feasible potential for diagnostic applications in clinical analysis of breast cancer gene BRCA1. Foreseeable, this sensor made on this basis undoubtedly provide the most effective and sensitive detection for BRCA1.
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PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2020
·
IF 5.065
Yushu Wu,Min Liu,Wenxin Pei,Yanna Zhao,Danfeng Wang,Tingting Liu,Bin Sun,Qingpeng Wang,Jun Han
DNA nanostructures-based chemotherapy-phototherapy (CTPT) combination therapy can achieve targeted release and effectively overcome multidrug resistance (MDR). The therapeutic effect of CTPT combination drugs loaded in DNA nanostructures is related to the interactions among them. Herein, based on our previous research on AS1411NTrs loading with a single drug of DOX , the interactions between a single drug (toluidine blue O, TBO) or combination drugs (doxorubicin and toluidine blue O, DOX + TBO) and AS1411 aptamer-tethered DNA nanotrains (AS1411NTrs) were investigated. By fluorescence spectroscopy and isothermal titration calorimetry technique, the corresponding thermodynamic parameters were obtained, including the number of binding sites, binding constant , enthalpy change and entropy change. By combining with the differential scanning calorimetry and ferrocyanide ion quenching analysis, the binding mode-thermodynamic parameters were correlated. In addition, the effect of the binding affinity of DOX and TBO to AS1411NTrs on the drugs release rate was studied. Finally, the in vitro cytotoxicity and confocal laser scanning microscopy imaging of AS1411NTrs + TBO + DOX was investigated. The results showed that this drug delivery system could increase its in vitro cytotoxicity and overcome drug resistance in DOX-resistant human breast cancer cells (MCF-7/ADR). The work would provide important information for the combination therapy of DOX and TBO to overcome MDR effectively.
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高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Xia Wang,Xuan Zhang,Cai Gao,Xiaolei Liao,Le Hang,Shulian Jiang,Feng Gao,Lizhang Huang,Qingxiang Wang
A novel electrochemical DNA biosensor based on in-situ labeling of L -Aspartic acid-copper ion (Asp-Cu 2+ ) complex on the terminal of molecule beacons (MBs) was constructed. The MB with bifunctional groups of 3’–SH and 5’–COOH was first anchored on a gold electrode surface through Au-S assembly chemistry, then L -Aspartic acid (Asp) was grafted with the 5’–COOH of MB via succinimide coupling, which was used as a platform molecule for the following coordination assembly of copper ions (Cu 2+ ). Therefore an electroactive MBs probe with the copper complex (Asp-Cu 2+ ) as a signal source was obtained. The hybridization between the loop sequence and the target DNA transferred the hairpin-like MBs to the linear form, and the electroactive moiety of Asp-Cu 2+ was forced to leave away from the electrode surface, resulting in the reduction of the electrochemical response of the biosensor. The hybridization experiment results showed that the oxidation peak currents of developed biosensor attenuated linearly with the logarithmic values of the target DNA concentrations in the kinetic range from 1.0×10 -15 ~1.0×10 -10 M. The detection limit was estimated to be 0.17 fM. Moreover, the biosensor showed excellent selectivity for discriminating the complementary sequences from the mismatched and noncomplementary sequences.
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高影响因子
Separation Science Plus
·
2018
·Vol. 1
·期 3
·217-224
·
IF 0
Lei Chen,Lei Wang,Dan Song,Zhangrun Xu
A three-dimensional reduced graphene oxide porous aerogel with packaged TiO 2 nanoparticles was prepared by a simple one-step self-assembly in a water bath at a mild temperature. Herein, we overcame the aggregation of graphene layers by forming three-dimensional architecture, and utilized TiO 2 aggregates as blocks to make the composite surface uneven. The extended aromatic areas contribute to DNA adsorption by aromatic stacking and hydrophobic interaction. The obtained composite exhibited porous structure and was positively charged at a low pH. The composite exhibited a high capacity of 890.1 mg g −1 and an adsorption efficiency of 98.4% to DNA at pH 3, however, bovine serum albumin at the same concentration level was hardly adsorbed. The composite showed favorable selectivity to DNA against protein even designed in such a simple manner. The retained DNA could be stripped by using a 0.04 mol L −1 Britton–Robinson buffer at pH 10, giving rise to a recovery of 96.3%. The DNA was isolated from human whole blood to be used as templates for PCR amplification successfully. This illustrates that DNA with high purity and quality was obtained.
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NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
·
2022
·
IF 1.449
Xin-Yan Lu,Yan-Yue Lou,Kai-Li Zhou,Shao-Liang Jiang,Jie-Hua Shi
In this paper, the interacting characteristics of febuxostat (FBST), an inhibitor of xanthine oxidase for treating gout patients with hyperuricemia with calf thymus DNA (ctDNA) was investigated through multi-spectroscopic methodologies combined with theoretical calculation for understanding the interacting mode on ctDNA, affinity with ctDNA, interacting forces, as well as the alteration in the conformation of ctDNA after interacting FBST The experimental results demonstrated that interacting FBST with ctDNA formed 1:1 complex, the association constant was 913 M−1 at 298 K, suggesting the affinity of FBST on ctDNA was very weak, the interacting mode of FBST on ctDNA was groove binding, and it inserted into the minor groove with rich A-T region of ctDNA. Based on the results of the thermodynamic analysis and theoretical calculation, it can be inferred that the dominated interacting forces between FBST and ctDNA were van der Waals forces and hydrogen bond. And, interacting FBST with ctDNA was a spontaneous, enthalpy-driven, and exothermic process because of ΔG0 < 0, ΔH0 < 0, and |ΔH0| > T|ΔS0|. The results of the circular dichroism (CD) measurements indicated the conformation of ctDNA was weakly disturbed after interacting with FBST but still maintained B-conform. The studied results offer significant insight into further clarifying whether it has genotoxicity.
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高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Weiwei Qiu,Feng Gao,Natsumi Yano,Yusuke Kataoka,Makoto Handa,Weiqiang Yang,Hidekazu Tanaka,Qingxiang Wang
Electrochemical aptasensors involved in chemical labeling are often single-use and sensitivity-limited because the probes are commonly single-point labeled and irreversible. In this work, the specific coordination between Zr4+ and phosphate group (−PO43–) was employed to construct a new aptasensor that is highly sensitive and reusable, using Ochratoxin A (OTA) as the test model. The OTA binding aptamer (OBA) was hybridized with the thiolated supporting sequence (TSS) immobilized on the surface of a gold electrode. The UiO-66 with a formula of [Zr6O4(OH)4(BDC)6], one of the class of Zr metal–organic frameworks (MOFs), was then particularly grafted on the terminal of OBA through the specific coordination between Zr4+ and 5′-PO43–, i.e., the Zr–O–P coordination bond. Similarly, as much as the 5′-PO43– and 3′-methylene blue dual-labeled sequences (DLS) were further assembled on UiO-66 due to the large surface area of MOF and rich active sites of Zr4+. Owing to the specific coordination for signal amplification, the developed aptasensor shows greatly enhanced sensitivity. A wide detection range from 0.1 fM to 2.0 μM and an ultralow detection limit of 0.079 fM (S/N = 3) for OTA were obtained. Additionally, the TSS can rehybridize with a new OBA to regenerate the aptasensor but without complicated pretreatments, enabling a aptasensor that is readily reusable for OTA detection. The aptasensor was successfully applied for OTA detection in the red wine samples, demonstrating a promising prospect for food safety monitoring.
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高影响因子
CELLULOSE
·
2024
·Vol. 31
·期 4
·2603-2615
·
IF 4.9
Wang Xinyu,Xu Qingbo,Zhang Junhua,Chen Qian
Conductive cotton fabrics are increasingly used in intelligent wearables and as electromagnetic shielding. Cotton fabrics can be endowed with conductive properties by immobilizing conductive inorganic substances on them or by coating them with conductive polymers; however, cracking of the coating results in a sharp decrease in its conductive properties and limits the applications. To solve this problem, a binder of carboxymethyl chitosan and l -cysteine was used to adhere DNA strands onto the cotton fabric as a “double helix” template for the formation of conductive silver nanoparticle strings. The DNA was used to adsorb silver ions (Ag + ); double-helix strings of silver nanoparticles (Ag NPs) were subsequently synthesized on the cotton fabric on exposure to a reducing agent. As a result of the double-helix structure, the Ag NP strings had a degree of elasticity and the conductive coating on the finished cotton fabric was very stable under the action of external forces. Even the finished fabric after withstand stretching, folding, or bending treatments, the surface resistance did not increase significantly. Finished fabrics prepared using this method therefore have excellent conductive stability, which considerably expands the range of application for conductive cotton fabrics.
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高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Jiaying Wang,Bin Li,Bo Yao,Yi Zhao,Kai Zhang
In this work, we propose a Ti 3 C 2 T x -based electrochemiluminescence (ECL) sensing platform, combined with a programmable entropy-driven cycling strategy and inverted DNA tetrahedral nanomachines to detect the SARS-CoV-2 RdRp gene. The use of Ti 3 C 2 T x in the ECL sensing platform provides unique advantages that improve the assay's sensitivity. Firstly, the SARS-CoV-2 RdRp gene triggers an entropy-driven cyclic amplification reaction to obtain a large number of Output strands. Subsequently, the Output strand reacts with an inverted DNA tetrahedral swing arm strand to start the self-driven process. Finally, the ferrocene (Fc)-labeled hairpin DNA strand of the inverted DNA tetrahedron is severed to achieve the signal change. Under optimal conditions, the ECL platform integrated with entropy-driven cycle amplification reaction and inverted DNA tetrahedral nanomachines obtained a wide range of 100 aM to 10 nM and a limit of detection (LOD) of 48.3 aM for the SARS-CoV-2 RdRp gene. Moreover, the recovery rates of 97% to 103.3% and 98.34% to 103% were obtained in real environments and human pharyngeal swabs, respectively. This ECL sensing platform, which combines an entropy-driven cycle amplification reaction and inverted DNA tetrahedral nanomachines, opens up new opportunities for the detection of other substances.
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高影响因子
危险品化学品经营许可证（带存储）