Advanced Science
·
2025
·
IF 14.1
Doudou Lei,Lin Liao,Tao Qin,Xiaoxuan Guan,Kunpeng Duan,Zhiwei Gao,Weiqian Jin,Mingjing Yin,Ke Zhang,Yan Liu,Yin Chen,Huyang Gao,Jiaxiao Li,Feifei Huang,Wenjing Liu,Chengde Xia,Bailei Wang,Hualin Huang,Shengqiu Lv,Qiang Zhi,Jiahao Huang,Ming Gao,Junyu Lu
Acute lung injury (ALI) refers to damage to lung related cells, typically caused by an uncontrollable inflammatory response, and over-generated reactive oxygen species (ROS). Increasing evidence suggests that reprogramming lung redox homeostasis holds significant potentials for the clinical treatment of ALI. Herein, the simple synthesis of ultra-small Pd loaded covalent organic framework (COF) (TP@Pd) is reported, which, when combined with near infrared (NIR) irradiation, exhibits nanozyme functionalities, including multiple enzyme mimicking activities and broad spectrum ROS scavenging, thereby promoting tissue repair for ALI immunotherapy. Mechanistically, through the therapeutic strategy of TP@Pd+NIR, the damaged cells and tissues are ameliorated by decreasing intracellular ROS levels (total ROS, ·OH and ·O 2 − ), downregulating inflammatory cytokines levels (IL-6, TNF-α and IL-1β), upregulating antioxidant factor level (SOD2), inducing macrophage M2 directional polarization (downregulation of iNOS and CD86, and upregulation of IL-10 and CD206), activating immunoregulation (CD4 + /CD8 + ratio increase), promoting tissue repair factor levels (upregulation of HSP70 and CD31), and suppressing the NF-κB signaling pathway (downregulation of phosphorylated p65 and IκBα). Furthermore, following intravenous (IV) injection in rats, TP@Pd accumulated in lung tissue for 6 h, indicating the promising therapeutic efficacy via this administration route. Notably, the TP@Pd+NIR strategy demonstrated the excellent synergistic effects in alleviating lung inflammation storms, reducing diffuse alveolar damage, and accelerating lung tissue repair. Summarily, this work has designed a novel TP@Pd+NIR strategy for the synergistic enhancement of ALI amelioration, which may serve as a promising therapeutic approach for other ROS related diseases.
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 10
·1-13
·
IF 5.3
Guo Liang,Zhou Shijin,Xue Jinyan,Liu Zenghui,Xu Shuqing,He Zhangxu,Yang Huaixia
A sandwich electrochemical sensor was fabricated based on multi-walled carbon nanotubes/ordered mesoporous carbon/AuNP (MWCNTs/CMK-3/AuNP) nanocomposites and porous core–shell nanoparticles Au@PdNPs to achieve rapid and sensitive detection of AFB 1 in complex matrices. MWCNTs/CMK-3/AuNP nanocomposite, which was prepared by self-assembly method, served as a substrate material to increase the aptamer loading and improve the conductivity and electrocatalytic activity of the electrode for the first signal amplification. Then, Au@PdNPs, which were synthesized by one-pot aqueous phase method, were applied as nanocarriers loaded with plenty of capture probe antibody (Ab) and signal molecule toluidine blue (Tb) to form the Au@PdNPs-Ab-Tb bioconjugates for secondary signal amplification. The sensing system could still significantly improve the signal output intensity even in the presence of ultra-low concentration target compound due to the dual signal amplification of MWCNTs/CMK-3/AuNP nanocomposites and Au@PdNPs-Ab-Tb. The method exhibited high selectivity, low detection limit (9.13 fg/mL), and strong stability to differentiate AFB 1 from other mycotoxins. Furthermore, the sensor has been successfully applied to the quantitative determination of AFB 1 in corn, malt, and six herbs, which has potential applications in food safety, quality control, and environmental monitoring. Graphical Abstract
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Qingcai Yu,Ying Chang,Shaohong Guo,Yaqiong Su,Jingchun Jia,Meilin Jia
In direct formic acid fuel cells, the formic acid oxidation reaction (FAOR) takes place at the anode, and the oxygen reduction reaction (ORR) occurs at the cathode. Whereas the cathode of zinc-air batteries (ZABs) also involves the ORR, these three electrochemical reactions collectively constitute the core processes of related energy devices. The study of the catalytic performance of palladium (Pd)-based catalysts has become a research focus. This study explores the utilization of rare earth metal-organic frameworks to enhance the interface interactions between Pd and CeO 2 . These frameworks facilitate the modulation of the electronic state of Pd, ensure site stability, and promote the generation of additional oxygen vacancies through bridge bonding between Pd–O–Ce. The Pd-CeO 2 /C(1:2) catalyst in 0.5 M H 2 SO 4 exhibited high initial potential (0.86 V vs. reversible hydrogen electrodes (RHE)), half-wave potential (0.76 V vs. RHE), excellent ORR stability, and exceptional performance in ZAB cathodes with a power density reaching 195.4 mW·cm −2 . Furthermore, in FAOR (0.5 M H 2 SO 4 + 0.5 M HCOOH) applications, this catalyst displays significantly higher activity (991.4 mA·mg −1 ) and stability than commercial Pd/C and platinum/C catalysts while maintaining excellent selectivity for formic acid decomposition. Theoretical calculations further prove that the formation of the Pd–O–Ce bridge bond is conducive to FAOR's direct reaction pathway and avoids CO toxicity. The Pd-CeO 2 /C(1:2) catalyst is utilized in positive and negative electrodes within self-assembled H-type electrolytic cells, achieving open-circuit voltages superior to those offered by commercial catalysts, thus confirming its practical applicability.
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiuzhen Li,Yuanting Ni,Chenxi Liu,Shaohua Xu,Wei Shu,Hong Liang,Ming Chen
Background Homocysteine (Hcy) has been recognized as an independent risk factor for the development of cardiovascular disease. Many electrochemical detection methods have been developed for analyzing Hcy, however, the specificity of these methods is based on the interaction between the sulfhydryl groups in Hcy and the metallic materials, and thus the specificity of such detection is not satisfactory. Results In this study, an electrochemical sensor based on COFs and Pd@CuO was developed for the specific detection of Hcy. Firstly, COFs material was used as an immobilized carrier for cystathionine β-synthase (CBS), and the immobilized CBS as a recognition unit was able to catalyze the production of H 2 S from the substrates (Hcy and Cys) in a specific manner. Secondly, the Pd@CuO composite material has excellent conductivity and can catalyze H 2 S as a source of electrochemical signal generation. The electrochemical sensor constructed based on the above principles has a good electrochemical response to Hcy within the range of 5 μM to 100 μM, and the limit of detection can reach 0.11 μM (S/N = 3). In addition, the constructed sensor has good reproducibility, stability and specificity, and has good recoveries in the detection of real plasma samples, which provides a potential application for the diagnosis of homocysteinemia. Significance and Novelty The electrochemical sensor significantly increases the specificity of detection by utilizing immobilized Hcy specific catalytic enzymes CBS. In addition, Pd modified CuO@COFs electrode greatly increases the conductivity of the electrode, thereby increasing the sensitivity of detection. These characteristics make the electrochemical sensor have good application prospects for detecting the Hcy in clinic in the future.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Li Liu,Yueyuan Li,Lihui Tian,Qin Wei,Wei Cao
A novel and ultrasensitive sandwich-type electrochemical immunosensor was designed for quantitative detection of prostate specific antigen (PSA). The Ag overgrowth in Pd nano-octahedrons, developed Pd-Ag heterodimers (Pd-Ag HDs), loaded on amino functionalized multiwalled carbon nanotubes (MWCNTs-NH 2 ) were employed as labels of secondary antibodies (Ab 2 ). The synergistic effect of Pd-Ag HDs exhibited high electrocatalytic activity towards the reduction of H 2 O 2 . MWCNTs-NH 2 with good dispersion , plentiful amino groups and large specific surface area , could load a large number of Pd-Ag HDs to enhance the sensitivity and amplify the signal of the immunosensor. Moreover, the electroplated gold nanoparticles (Au NPs) were employed for the immobilization of primary antibodies (Ab 1 ). Au NPs with excellent conductivity could enhance the electron transport between substrate material and electrode surface , and improve the sensitivity of immunosensor. Under optimal conditions, the electrochemical immunosensor exhibited a wide linear range of 0.1 pg/mL–30 ng/mL towards PSA, and a low detection limit of 0.03 pg/mL (S/N = 3). The proposed immunosensor exhibited good sensitivity, reproducibility and stability for the quantitative detection of PSA, which hold a great potential in clinical and diagnostic applications.
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高影响因子
ONCOLOGY REPORTS
·
2024
·Vol. 52
·期 4
·1-8
·
IF 3.8
Tong Wang,Xu Ye,Hao Jiang,Yu Gao
B‑cell lymphoma is difficult to cure because of its biological and clinical heterogeneity, and due to native chemoresistance. Immunotherapies that overcome cancer‑induced immune evasion have been the center of recent developments in oncology. This is emphasized by the accomplishment of various agents that disrupt programmed cell death protein 1 (PD‑1)‑mediated immune suppression in diverse tumors. However, while PD‑1 blockade has been effective in numerous malignancies, a significant proportion of cancers, including B‑cell lymphoma, show certain rates of primary resistance to these therapeutic strategies. Histone deacetylase inhibitors (HDACis) have exhibited anticancer activity though suppressing cell proliferation, inducing differentiation and triggering apoptosis. The present study aimed to explore a therapeutic strategy combining a HDACi (romidepsin) and PD‑1 blockade (BMS‑1) in B‑cell lymphoma, utilizing a constructed mouse model of B‑cell lymphoma. The IC50 of the two inhibitors was confirmed by MTT assay, and their inhibitory effects were revealed to be dose‑ and time‑dependent. The data demonstrated that the combined treatment of romidepsin and BMS‑1 synergistically inhibited the growth of B‑cell lymphoma. Furthermore, it was revealed that romidepsin and BMS‑1 synergistically triggered apoptosis in mouse B‑cell lymphoma. The synergistic effect of these agents was capable of activating tumor‑infiltrating lymphocytes, particularly CD3+CD4+ and CD3+CD8+ T cells. The results of the present study underscore the potential of HDAC inhibition in conjunction with PD‑1 blockade as a novel therapeutic approach for B‑cell lymphoma, highlighting the synergistic effects of these two mechanisms in enhancing antitumor immunity.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 17
·7035-7041
·
IF 3.288
Mufei Liu,Hao Yang,Zewen Xu,Wenlu Ma,Fang Cui,George Lu,Linxu Xu,Tieyu Cui
Based on green chemistry, 3D hierarchical ZnO microflowers (MFs) constructed from layered 2D nanosheets have been designed as carriers to uniformly load PdO/Pd nanoparticles by the calcination of the precursor (ZnO/Pd(II) microflowers) in air. The ZnO/Pd(II) microflowers are prepared by dipping the 3D ZnO MFs in an ethanolic solution of PdCl2 without involving additional agents. The as-prepared 3D hierarchical ZnO microflowers are a stable support to enhance the dispersibility of PdO/Pd nanoparticles to achieve better catalytic performance for the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP). In addition, the synergistic effect between the PdO and Pd nanoparticles is another major driver that promotes the catalytic process. Over 98% of 4-NP is reduced within 7 min. Furthermore, structural analysis of the ZnO/PdO/Pd microflowers can provide useful information for understanding structure-performance relationships.
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2016
·
IF 2.653
Huan Wu,Yanmei Zhao,Xuanping Tan,Xiaoqing Zeng,Yuan Guo,Jidong Yang
A convenient fluorescence quenching method for determination of Quizalofop-p-ethyl(Qpe) was proposed in this paper. Eosin Y(EY) is a red dye with strong green fluorescence (λex/λem = 519/540 nm). The interaction between EY, Pd(II) and Qpe was investigated by fluorescence spectroscopy, resonance Rayleigh scattering(RRS) and UV–Vis absorption. Based on changes in spectrum, Pd(II) associated with Qpe giving a positively charged chelate firstly, then reacted with EY through electrostatic and hydrophobic interaction formed ternary chelate could be demonstrated. Under optimum conditions, the fluorescence intensity of EY could be quenched by Qpe in the presence of Pd(II) and the RRS intensity had a remarkable enhancement, which was directly proportional to the Qpe concentration within a certain concentration range, respectively. Based on the fluorescence quenching of EY-Pd(II) system by Qpe, a novel, convenient and specific method for Qpe determination was developed. To our knowledge, this is the first fluorescence method for determination of Qpe was reported. The detection limit for Qpe was 20.3 ng/mL and the quantitative determination range was 0.04–1.0 μg/mL. The method was highly sensitive and had larger detection range compared to other methods. The influence of coexisting substances was investigated with good anti -interference ability. The new analytical method has been applied to determine of Qpe in real samples with satisfactory results.
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高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Shuang Wu,Zheni Wang,Lei Wan,Chenghui Luo,Shams Ali Baig,Xinhua Xu
In the present study, Pd/Ni foam electrode was prepared by improved impregnation method for electrocatalytic hydrodechlorination (ECH) of clofibric acid (CA). The effects of complexing agent addition into the impregnation solution for the formation of Ni(OH) 2 and the ECH activity of Pd/Ni foam electrode were investigated. Results revealed that the introduction of sodium acetate (the complexing agent) greatly enhanced the ECH activity as compared to common impregnation liquid. Characterizations analyses demonstrated that the addition of sodium acetate did not change the phase of the electrode’s surface, but effectively reduced the deposition of Ni(OH) 2 . The surface morphology of the electrode was changed simultaneously by the addition of sodium acetate and the interlacing structure of Ni(OH) 2 and Pd catalyst was formed. NaAc before -Pd/Ni and NaAc after -Pd/Ni foam electrodes were used to perform ECH experiments on CA, and the combined Pd/Ni foam 15 min electrode was compared with the Pd/Ni foam 6 h electrode. After 2 h of electrolysis, the NaAc after -Pd/Ni foam electrode was found to remove the CA (∼100%) in the solution, which was 1.3 times the removal rate of the Pd/Ni foam 6 h electrode to CA and exceeded the Pd/Ni foam 15 min electrode. Findings from the present study demonstrated that adding the complexing agent into the impregnation solution can improve the ECH activity of Pd/Ni foam electrode and achieve a faster and more efficient dechlorination effect of CA.
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高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2017
·Vol. 31
·期 12
·e3854
·
IF 2.319
Xiaojun Ma,Guanghui Lv,Xu Cheng,Weijian Li,Rui Sang,Yong Zhang,Qiantao Wang,Li Hai,Yong Wu
An environmentally friendly palladium(II) catalyst supported on cyclodextrin-modified h-BN was successfully prepared. The catalyst was characterized by FT-IR, SEM, TG, XRD and XPS, and the loading level of Pd in h-BN@β-CD@Pd(II) was measured to be 0.088 mmol g −1 by ICP. It exhibits excellent catalytic activity for the Suzuki and Heck reactions in water, and can be easily separated and consecutively reused for at least nine times. In addition, a series of pharmacologically interesting products were successfully synthesized using this catalyst to demonstrate its potential applications in pharmaceutical industries. Above all, this work opens up an interesting and attractive avenue for the use of cyclodextrin-functionalized h-BN as an efficient support for hydrophilic heterogeneous catalysts.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 31
·14879-14887
·
IF 3.925
Fu Wang,Chunping Li,Tong Xu,Ying Li,Yinghui Sun,Jie Bai
Among rare earth oxides (REO), mixed-valence praseodymium oxide (Pr3+/Pr4+) has the highest O mobility and very promising applications in multiphase catalysis. In this paper, Pd-loaded praseodymium oxide nanotubes were successfully prepared by immobilizing Pd nanoparticles on modified carriers. XPS characterization showed that the electron transfer process during the reaction could be regulated by changing the Pr3+/Pr4+ ratio of praseodymium oxide. Importantly, the H2-PrxOy carrier surface has much higher Pd electron density, and the electron-rich Pd nanoparticles make it easier to break the C–Br bond and transfer electrons from Pd to C and Br atoms, leading to the formation of ArPdIIBr intermediate complexes. In the Suzuki coupling reaction, Pd/H2-PrxOy exhibited higher catalytic performance under mild conditions. There is a strong correlation between the surface properties of the carriers and the catalytic activity of the Pd nanocatalysts. This provides a new strategy for the preparation of Suzuki coupling reaction catalysts.
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高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2013
·Vol. 97
·期 17
·7597-7605
·
IF 3.689
Xia Jun,Xu Hong,Feng Xiaohai,Xu Zhaoxian,Chi Bo
Poly(ε- l -lysine) (ε-PL) producer strain Streptomyces albulus PD-1 secreted a novel polymeric substance into its culture broth along with ε-PL. The polymeric substance was purified to homogeneity and identified. Matrix-assisted laser desorption ionization-time of flight mass spectrometry and nuclear magnetic resonance spectroscopy as well as other analytical techniques revealed that the substance was poly( l -diaminopropionic acid) (PDAP). PDAP is an l -α,β-diaminopropionic acid oligomer linking between amino and carboxylic acid functional groups. The molecular weight of PDAP ranged from 500 to 1500 Da, and no co-polymers composed of l -diaminopropionic acid and l -lysine were present in the culture broth. Compared with ε-PL, PDAP exhibited stronger inhibitory activities against yeasts but weaker activities against bacteria. ε-PL and PDAP co-production was also investigated. Both ε-PL and PDAP were synthesized during the stationary phase of growth, and the final ε-PL and PDAP concentration reached 21.7 and 4.8 g L -1 , respectively, in fed-batch fermentation. Citric acid feeding resulted in a maximum ε-PL concentration of 26.1 g L -1 and a decrease in the final concentration of PDAP to 3.8 g L -1 . No studies on ε-PL and PDAP co-production in Streptomyces albulus have been reported previously, and inhibition of by-products such as PDAP is potentially useful in ε-PL production.
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高影响因子
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