Scientific Reports
·
2019
·Vol. 9
·期 1
·1-10
·
IF 4.011
Liu Jiakuan,Fang Tian,Li Meiqian,Song Yuting,Li Junzun,Xue Zesheng,Li Jiaxuan,Bu Dandan,Liu Wei,Zeng Qinghe,Zhang Yidan,Yun Shifeng,Huang Ruimin,Yan Jun
Benign prostatic hyperplasia (BPH) is one of the most common diseases in the urinary system of elderly men. Pao extract is an herbal preparation of the bark of the Amazon rainforest tree Pao Pereira ( Geissospermum vellosii ), which was reported to inhibit prostate cancer cell proliferation. Herein we investigated the therapeutic potential of Pao extract against BPH development in a testosterone-induced BPH rat model. The administration of testosterone induced the prostate enlargement, compared with the sham operated group with vehicle treatment. The BPH/Pao group showed reduced prostate weight comparable with BPH/finasteride group. Notably, Pao treatment did not significantly reduce body weights and sperm number of rats, compared with the control group. Furthermore, Pao extract treatment reduced the proliferative index in prostate glands and testosterone-induced expression levels of AR, as well as androgen-associated proteins such as SRD5A1 and PSA. Moreover, Pao extract and its active component, flavopereirine, induced cytotoxicity on human prostate epithelial RWPE-1 cells in a dose- and time- dependent manner with G2/M arrest. Consistently, Pao extract and flavopereirine suppressed the expression levels of SRD5A1, AR and PSA, respectively. Together, these data demonstrated that Pao extract suppresses testosterone-induced BPH development through inhibiting AR activity and expression, and suggested that Pao extract may be a promising and relative safe agent for BPH.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2020
·Vol. 412
·期 3
·611-620
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IF 3.637
Wang Chengnan,Gao Xia,Wang Shuo,Liu Yaqing
Infections caused by foodborne microorganisms are a great threat to the global environment and public healthcare today. Thus, rapid, portable and sensitive assays that can realize the identification of foodborne bacteria are highly desired. In this study, a smart fluorescent and colorimetric dual-readout sensing system has been established for simple and rapid E. coli determination by utilizing the Cu 2+ -triggered oxidation of o -phenylenediamine (OPD). Initially, Cu 2+ can oxidize OPD to OPDox, resulting in an orange-yellow fluorescence and visible pale-yellow color. However, E. coli can effectively reduce Cu 2+ into Cu + , inhibiting the Cu 2+ -triggered oxidation of OPD to OPDox. Consequently, the introduction of E. coli can turn off both the fluorescence and the UV-vis absorbance signals of the OPD-Cu 2+ system, illustrating an original mechanism for fluorescent and colorimetric dual-channel detection of E. coli . Moreover, a filter paper-based visual sensor was built and coupled with OPD-Cu 2+ solution under the assistance of a UV lamp. The as-prepared sensor can detect E. coli quantitatively with the help of a typical smartphone color-scanning application (APP). Thus, this study offers a valid dual-mode assay for sensitive and on-site visible detection of E. coli , guaranteeing the reliability of the results and is more attractive for practical use.
Schematic illustration of the smartphone-integrated sensing system for fluorescent and colorimetric dual-channel detection of E. coli based on the Cu 2+ -OPD system.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2020
·Vol. 22
·期 1
·1-15
·
IF 2.132
Ding XiangYu,Cao Qi,Zhu Sheng,Xu Qunjie,Min Yulin
A bio-inspired strategy has been proposed to synthesize a unique kind of 3D porous cobalt oxide and carbon nanosheet composite materials (PCN@Co 3 O 4 ), as a lithium-ion battery anode material. In this work, turnip has been chosen as a two-dimensional nanobiomass carbon substrate and choosing cobalt salt as the metal precursor by using plant cell suction phenomenon of dehydration to prepare PCN@Co 3 O 4 . When PCN@Co 3 O 4 is applied for the anode material for lithium-ion batteries, it delivers ultrahigh reversible capacity and excellent electrochemical performance. After 100 cycles, PCN@Co 3 O 4 has a reversible specific capacity of 1004.2 mA h g −1 at a current density of 100 mA g −1 and a reversible capacity of about 923.7 mA h g −1 after 400 cycles even at a high current density of 1000 mA g −1 . Furthermore, it also exhibits a high rate capability, which can be stabilized at about 1151, 939.7, 771.4, and 631.5 mA h g −1 for every 10 successive cycles when increasing the current density to 100, 500, 1000, and 1500 mA g −1 , respectively. And a satisfactory-specific capacity can be recovered to the original value (about 1150 mA h g −1 ) at a current density of 100 mA g −1 after 60 discharge/charge cycles.
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JOURNAL OF MATERIALS SCIENCE
·
2019
·Vol. 55
·期 11
·4578-4591
·
IF 3.442
Diao Shuai,Liu Huie,Chen Shuang,Xu Wenlong,Yu Anran
Graphene aerogels with different pore sizes, volumes and degrees of reduction were prepared through Pickering emulsion method. The controllable preparation of aerogels was achieved. The graphene aerogels prepared were characterized by FTIR and XPS. The adsorption capacity of pure oil by graphene aerogel was investigated. The graphene aerogels can adsorb pure oil quickly and hardly adsorb water. Linear relation exists between saturated adsorption capacities of graphene aerogels on oil and the densities of the oil adsorbed. The volume of the adsorbed organics on unit mass of the aerogel is 186.46 cm 3 g −1 . The occupancy rate in the aerogel pores is 73.71%. The factors affecting its adsorption rate on emulsified oil were investigated. The adsorption curves of graphene aerogels on emulsified oil conform to the pseudo-second-order kinetic model. The larger the inner diameter of the pore size in the graphene aerogels and the larger the outer surface area, the faster the adsorption rate of the emulsified oil would be. The adsorption rate of the rough internal with higher hydrophobicity decreases slightly, but as the degree of reduction increases, the hydrophobicity gradually increases and the adsorption rate increases gradually, so there should exist an optimum hydrophobicity value of aerogels for the adsorption of emulsified oil in water.
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POLYMER BULLETIN
·
2019
·Vol. 77
·期 9
·4697-4711
·
IF 1.858
Ma Yangmin,Lei Rui,Jiang Yajuan
Abstract Waterborne alkyd resins based on Zanthoxylum bungeanum seed oil modified with epoxy resin were prepared by polyesterification reaction. The different base catalysts were examined on the alcoholysis reaction. Further, epoxy-modified waterborne alkyd resins were blended with hexamethoxy methyl melamine to improve the properties like curing time, chemical resistance and thermal stability. The properties of the prepared resins (acid value, drying time, pencil hardness, adhesion, flexibility, chemical resistance and thermal stability) were studied. The prepared polymers were characterized by FT-IR spectroscopy, particle size analysis and thermogravimetric analysis. ZSO-based alkyd resins modified epoxy resin and their blends with HMMM (EWA:HMMM = 3:1) showed enhanced chemical resistance, physical properties and thermal stability. Graphic abstract
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Journal of Food Measurement and Characterization
·
2019
·Vol. 14
·期 2
·694-707
·
IF 1.415
Yin Xing-shuo,Zhang Xue-qin,Li De-qiang
In this study, the ultrasound-assisted extraction (UAE) of flavonoids with maximal antioxidant and tyrosinase inhibitory activities and identification of the principal individual flavonoids compounds of Semen Oroxyli by UHPLC-DAD-QTOF-MS/MS was proposed. The method was optimized by a three-factor-three-level Box–Behnken design (BBD) and Derringer’s desirability function using methanol concentration, extraction time, and liquid/solid ratio as factors, while, the total flavonoids content (TFC), antioxidant activities (DPPH, ABTS, FRAP, superoxide anion and hydroxyl radicals) and tyrosinase inhibitory activity as responses. The experimental results showed that the optimal extraction conditions were as followed: a solvent of 69.90% methanol, an extraction time of 30.64 min and a liquid/solid ratio of 31.30 mL/g, which give individual response values of 19.79 mg RE/g, 78.66%, 99.92%, 19.05 mM FE/g, 20.49%, and 13.37% for TFC, DPPH, ABTS, FRAP, superoxide anion and hydroxyl radicals, respectively. In addition, the tyrosinase inhibition assay showed that the extract of Semen Oroxyli exhibited strong inhibitory activity against the tyrosinase. There were no significant differences between the results of the optimization experiments and those predicted by the models. By UHPLC-DAD-QTOF-MS/MS analysis, the main flavonoids identified in the optimized UAE extracts of Semen Oroxyli were oroxin B, oroxin A, chrysin-7- O -β- d -glucuronid, baicalein and chrysin.
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CELLULOSE
·
2019
·Vol. 27
·期 2
·657-676
·
IF 3.917
Wang Hong,Pudukudy Manoj,Ni Yonghao,Zhi Yunfei,Zhang Heng,Wang Zhenquan,Jia Qingming,Shan Shaoyun
Abstract A single-step ammonium persulfate (APS)-assisted swelling, followed by oxidation, can prepare nanocrystalline cellulose (NCC) from cotton linters. The APS-swelling is the critical step in the process, and the effects of swelling time, temperature and solid–liquid ratios were thoroughly investigated. The optimal conditions for NCC preparation were a swelling time of 3.0 h, a swelling temperature of 25 °C, and a solid–liquid ratio of 1:50. Upon heating at 60 °C, the persulfate enters the amorphous region of the cellulose and produces active SO
·− 4
and H 2 O 2 , which effectively attack the two-phase structure of cellulose and oxidize the –OH group at the C-6 position. The swelling temperature of 25 °C plays a crucial role in breaking the hydrogen bonds between the molecular chains of cellulose. It permits the preparation of NCC with a high yield and crystallinity index. The crystalline structure of cellulose I β did not change after APS swelling and oxidation. The atomic force microscopic analysis confirmed the formation of spindle-shaped particles with a helical structure. Upon natural evaporation of the NCC suspension, brittle films were obtained, which exhibited a left-hand layered structure and high iridescence with a fingerprint-texture. These materials can be applied as strength additives and chiral templates. Graphic abstract
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Cell Death & Disease
·
2019
·Vol. 10
·期 11
·1-16
·
IF 5.959
Tan Peng,Xu Yunke,Du Yichao,Wu Lile,Guo Bing,Huang Shiyao,Zhu Jinhui,Li Bo,Lin Fujun,Yao Lei
Speckle-type POZ domain protein (SPOP), an adaptor in the E3 ubiquitin ligase complex, recognizes substrates and promotes protein degradation via the ubiquitin-proteasome system. It appears to help regulate progression of several cancers, and we show here that it acts as a tumor suppressor in pancreatic cancer. Our analysis of patient tissues showed decreased SPOP expression, which was associated with poor prognosis. SPOP knockdown in SW1990 (in vitro/vivo) and PANC-1 (in vitro) cells led to significantly greater proliferation, migration, and invasion. Co-immunoprecipitation experiments in SW1990 cells showed that SPOP interacted with the stem-cell marker NANOG, and this interaction has recently been shown to play a critical role in regulating progression of prostate cancer. We showed that, in one patient with pancreatic cancer, the expression of a truncated form of SPOP (p.Q360*) lacking the nuclear localization signal led to nuclear accumulation of NANOG, which promoted growth and metastasis of pancreatic cancer cells. Our results suggest that SPOP suppresses progression of pancreatic cancer by promoting the ubiquitination and subsequent degradation of NANOG. These results identify the SPOP-NANOG interaction as a potential therapeutic target against pancreatic cancer.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2019
·Vol. 55
·期 6
·2471-2481
·
IF 3.442
Ji Lijun,Zhou Shu,Liu Xi,Gong Mindong,Xu Tong
PAN–COOH fibers were prepared with PAN fibers and used as templates, carbon source and nitrogen source for the first time to prepare carbon- and nitrogen-doped TiO 2 /carbon (C, N–TiO 2 ) composite fibers. The C, N–TiO 2 composite fibers were characterized by TEM, SEM, XPS, XRD, IR, Raman and UV–Vis spectrum. The composite fibers exhibited strong photocatalytic activity in photodegradation of Rh B solution under UV–Vis light. Doping C and N into TiO 2 materials could reduce the band gap width of TiO 2 and expand the light absorption range. The carbon fiber inside the composite fiber promoted the transfer of photoelectrons and inhibited the combination of photogenerated electrons and holes. The large specific surface area of C, N–TiO 2 composite fibers was also conducive to the photocatalytic activity. The macroscale C, N–TiO 2 composite fibers were easy for recycling. Therefore, the C, N–TiO 2 composite fibers had the potential for dealing with organic pollutants in water.
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Communications Biology
·
2019
·Vol. 2
·期 1
·1-11
·
IF 0
Lin Yanke,Wang Shuai,Zhou Ziyue,Guo Lianxia,Yu Fangjun,Wu Baojian
Metabolism is a major defense mechanism of the body against xenobiotic threats. Here we unravel a critical role of Bmal1 for circadian clock-controlled Cyp3a11 expression and xenobiotic metabolism. Bmal1 deficiency decreases the mRNA, protein and microsomal activity of Cyp3a11, and blunts their circadian rhythms in mice. A screen for Cyp3a11 regulators identifies two circadian genes Dbp and Hnf4α as potential regulatory mediators. Cell-based experiments confirm that Dbp and Hnf4α activate Cyp3a11 transcription by their binding to a D-box and a DR1 element in the Cyp3a11 promoter, respectively. Bmal1 binds to the P1 distal promoter to regulate Hnf4α transcriptionally. Cellular regulation of Cyp3a11 by Bmal1 is Dbp- and Hnf4α-dependent. Bmal1 deficiency sensitizes mice to toxicities of drugs such as aconitine and triptolide (and blunts circadian toxicity rhythmicities) due to elevated drug exposure. In summary, Bmal1 connects circadian clock and Cyp3a11 metabolism, thereby impacting drug detoxification as a function of daily time.
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CLAYS AND CLAY MINERALS
·
2019
·Vol. 67
·期 4
·275-282
·
IF 1.835
Liu Xiaonan,Zhang Youkui,Bian Liang,Li Jinshan
The adsorption of bovine serum albumin (BSA) protein is important for protein research but remains a great challenge. Here, the hydrothermal method and calcination in a hydrogen-argon mixed atmosphere were used to obtain ordered mono-crystalline magnetic Fe 3 O 4 nanorings with controllable size and uniform structure, which were applied to the adsorption of BSA protein. The results showed that the magnetic Fe 3 O 4 nanorings prepared have the advantages of good crystallinity, controllable morphology, and excellent magnetic response. The Fe 3 O 4 nanorings had an outer diameter of ~160–180 nm, inner diameter of ~80–110 nm, and height of 70 to ~110 nm. The amount of BSA adsorbed by magnetic Fe 3 O 4 nanorings increased with increasing protein concentration; when the concentration of BSA was ~2.75 mg·mL -1 the adsorption amount approached saturation. When the pH value of the solution was ~5, the Fe 3 O 4 nanorings absorption of BSA was greatest. With increasing adsorption time, the amount adsorbed increased gradually. Adsorption equilibrium was reached after 3 h. According to the formula, the saturated adsorption capacity of BSA per gram of magnetic Fe 3 O 4 nanorings was 325.2 mg.
.
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MICROCHIMICA ACTA
·
2019
·Vol. 187
·期 1
·1-9
·
IF 5.479
Sun Chao,Zhao Shiyu,Qu Fei,Han Wenli,You Jinmao
A thiol-labeled adenosine triphosphate (ATP) binding aptamer is covalently linked on the surface of gold nanoparticles (AuNPs). This warrants protection of the red AuNPs from aggregation in high salt condition. The dispersed AuNPs can quench the fluorescence of the Tb(III)-MOFs at 547 nm with the excitation wavelength of 290 nm. This is ascribed to the combined action of inner filter effect, dynamic quenching and fluorescence resonance energy transfer. If the aptamer binds ATP to form folded structures, the AuNPs aggregate in high salt medium and the green fluorescence of the Tb(III)-MOFs is recovered. This method shows good sensitivity and selectivity for ATP, and the linear range is from 0.5 to 10 μM of ATP with the detection limitat of 0.32 μM. It was applied to the determination of ATP in (spiked) human plasma with satisfactory recoveries (from 93.2% to 106.3%). Oppositely, when the unlabeled aptamer is used instead of thiol-labeled aptamer in this process, the ATP-aptamer complexes rather than unlabeled aptamer provide greater protection for AuNPs against salt-induced aggregation. It is found that when the aptamer covalently binds to AuNPs, the steric hindrance is dominant for the stabilization of AuNPs; for unlabeled aptamer, the electrostatic repulsion is responsible for their stability, irrespective of whether ATP is present or not. These two different forces lead to the aggregation or dispersion of AuNPs with addition of target in salt solution.
The impact of two repulsive forces (electrostatic repulsion and steric repulsion) on the stabilization of gold nanoparticles, and its application in fluorescent terbium metal-organic frameworks as a nanoprobe for adenosine triphosphate.
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