BMC Complementary Medicine and Therapies
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2020
·Vol. 20
·期 1
·1-17
·
IF 0
Wang Le,Tan Nana,Wang Huan,Hu Jingbo,Diwu Wenbo,Wang Xiaoling
Background Flavonoids from plant medicines are supposed to be viable alternatives for the treatment of type 2 diabetes (T2D) as less toxicity and side effects. Radix scutellariae ( RS ) is a widely used traditional medicine in Asia. It has shown great potential in the research of T2D. However, the pharmacological actions remain obscured due to the complex chemical nature of plant medicines. Methods In the present study, a systematic method combining ultrafiltration UPLC-TripleTOF-MS/MS and network pharmacology was developed to screen α -glucosidase inhibitors from flavonoids of RS , and explore the underlying mechanism for the treatment of T2D. Results The n -butanol part of ethanol extract from RS showed a strong α -glucosidase inhibition activity (90.55%, IC 50 0.551 mg/mL) against positive control acarbose (90.59%, IC 50 1.079 mg/mL). A total of 32 kinds of flavonoids were identified from the extract, and their ESI-MS/MS behaviors were elucidated. Thirteen compounds were screened as α -glucosidase inhibitors, including viscidulin III, 2′,3,5,6′,7-pentahydroxyflavanone, and so on. A compound-target-pathway (CTP) network was constructed by integrating these α -glucosidase inhibitors, target proteins, and related pathways. This network exhibited an uneven distribution and approximate scale-free property. Chrysin ( k = 87), 5,8,2′-trihydroxy-7-methoxyflavone ( k = 21) and wogonin ( k = 20) were selected as the main active constituents with much higher degree values. A protein-protein interaction (PPI) weighted network was built for target proteins of these α -glucosidase inhibitors and drug targets of T2D. PPARG ( C d = 0.165, C b = 0.232, C c = 0.401), ACACB ( C d = 0.155, C b = 0.184, C c = 0.318), NFKB1 ( C d = 0.233, C b = 0.161, C c = 0.431), and PGH2 ( C d = 0.194, C b = 0.157, C c = 0.427) exhibited as key targets with the highest scores of centrality indices. Furthermore, a core subnetwork was extracted from the CTP and PPI weighted network. Type II diabetes mellitus (hsa04930) and PPAR signaling pathway (hsa03320) were confirmed as the critical pathways. Conclusions These results improved current understanding of natural flavonoids on the treatment of T2D. The combination of ultrafiltration UPLC-TripleTOF-MS/MS and network pharmacology provides a novel strategy for the research of plant medicines and complex diseases.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2020
·
IF 4.192
Yu Wang,Xiaoxiao Li,Hangyan Ji,Danni Zheng,Zhengyu Jin,Yuxiang Bai,Birte Svensson
Gelation of starch is a process during short-term retrogradation. However, long-term retrogradation always leads to the quality deterioration of starch-based food. In this work, a new type of modified tapioca starch (MTS) gel with maintained short-term gelation strength and retarded long-term retrogradation was prepared using a novel recombinantly produced and characterized 4-α-glucanotransferase (TuαGT). In the resulting MTS, the exterior chains of the amylopectin part were elongated and the content of amylose was reduced because of the disproportionation activity of TuαGT. The retrogradation analysis demonstrated that the MTS possessed highly weakened long-term retrogradation characteristics as compared to the native starch. Most importantly, the strength of the gel formed by regelatinized MTS is very close to that of gelatinized native tapioca starch when storing below 30 °C. These findings provide a starting point for developing a novel method for the enzymatic modification of the starch-based gels.
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CHEMICAL ENGINEERING JOURNAL
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2019
·
IF 8.355
Ruiyang Zhang,Tao Ran,Yuehan Cao,Liqun Ye,Fan Dong,Qian Zhang,Lizhi Yuan,Ying Zhou
Photocatalytic NO x removal is regarded as a promising technique for air purification in recent years. However, NO x removal process usually accompanies with toxic NO 2 generation, leading to serious secondary pollutant and limiting its practical application. Herein, a noble-metal-free g-C 3 N 4 /α-Ni(OH) 2 photocatalyst was fabricated through hydrothermal method. The introduction of α-Ni(OH) 2 microspheres combines with g-C 3 N 4 , resulting in an enlarged surface area from 35 to 95 m 2 /g, while the unique structure of α-Ni(OH) 2 broadens visible-light response range and accelerates photogenerated charge carriers transfer. More importantly, the H atoms of α-Ni(OH) 2 improve the adsorption and activation of O 2 and oxygen atoms to promote NO and NO 2 oxidized to nitrate. Consequently, g-C 3 N 4 /α-Ni(OH) 2 shows enhanced NO removal ratio of 51.3% without any toxic NO 2 generation, indicating its great potential for air purification. Our results provide fresh insight into the design of highly efficient and safe photocatalyst for toxic byproduct control in the application of environmental remediation.
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ACS Omega
·
2019
·
IF 2.584
Bo Cao,Chunhua Luo,Jie Lao,Hanqing Chen,Ruijuan Qi,Hechun Lin,Hui Peng
Layered cobalt hydroxides are cost-efficient electrocatalysts for oxygen evolution reaction (OER) in the field of energy conversion. Herein, we developed a facile synthesis method of 3d transition-metal-doped α-Co(OH)2 nanomaterials mediated with ammonia in water–methanol at room temperature. The doping of Cu2+ and Ni2+ leads to flower-like nanostructures similar to pure α-Co(OH)2, whereas the doping of Fe2+ produces nanoparticles with more than 2 times larger surface area in comparison with the Cu2+- and Ni2+-doped nanoflowers. The obtained dispersion with the addition of Nafion can be used directly as an electrocatalyst for OER with excellent catalytic activity, especially that the overpotential of Fe2+ doped is as low as 290 mV at 10 mA cm–2 and the turnover frequency is improved by 3 times as compared with that of α-Co(OH)2. Furthermore, the catalyst can be loaded onto foam nickel, which presents excellent durability with the current density unchanged under continuous chronoamperometry reaction for as long as 12 h and almost quantitative faradaic efficiency. The superior electrocatalytic properties combined with the simple synthesis without the tedious purification procedure is very promising for OER.
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INORGANIC CHEMISTRY
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2022
·
IF 5.436
Shan Jiang,Xuemei Jia,Jing Cao,Haili Lin,Fang Li,Yue Sun,Shifu Chen
Photocatalytic overall water splitting simultaneously generates O2 and H2; this is a potential strategy to solve the energy shortage problem. Elemental phosphorus (RP) displays ultralow visible light performance for O2 and H2 generation; thus, a novel α-Fe2O3/RP composite is designed and prepared by a low-temperature hydrothermal method via loading a trace amount of α-Fe2O3. In the experiment, the 1.5% α-Fe2O3/RP composite showed the best overall water splitting performance, which is 6.9 times that of bare RP. Through various characterization studies, the recombination rate of charges is significantly reduced. It is largely ascribed to the matched energy band structure of the two photocatalysts and the interface contact between α-Fe2O3 and RP, which efficiently separates the photocarriers through an S-scheme mode and realizes the obvious enhancement of overall water splitting performance. Moreover, α-Fe2O3/RP maintains high activity when it is persistently irradiated for 15 cycles. The research provides insight into the exploitation of low-cost, high-activity, and stable RP-based photocatalysts to achieve visible light induced overall water splitting activity to generate O2 and H2.
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MACROMOLECULES
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2023
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IF 5.5
Xinyan Yu,Yong Wang,Yutong Dong,Ning Zhao,Lifeng Zhang,Sunting Xuan,Zhengbiao Zhang
N-Methyl peptides, which are widely found in nature, have emerged as an intriguing material in both polymer science and the biomedical field. The inefficient synthesis of N-methyl peptides, however, has greatly impeded the progress toward their structure–property investigation and applications. Here, we report an efficient, moisture-tolerant, primary amine-initiated, and acetic acid-catalyzed ring-opening polymerization of enantiomerically pure N-methyl-α-amino acids N-carboxyanhydrides, yielding well-defined, stereoregular, and structurally diverse N-methyl polypeptides. The concentration of the acetic acid, polarity of solvents, and stiffness of polymer chains were found to be crucial to the rate of the polymerization. The acetic acid was proposed to activate the monomer through hydrogen bonding interaction, promote the release of CO2, and form an acid–base equilibrium with the secondary amine chain end. The obtained N-methyl polypeptides exhibited stable helix structures, revealing chain stiffness, consistent with the high glass transition temperatures (>250 °C) from DSC measurements. Moreover, preliminary studies indicated that N-methyl polypeptides are nontoxic both in vitro and in vivo and are enzymatically stable toward trypsin treatment. Together, this study provides a mild and efficient method for the preparation of N-methyl polypeptides, opening avenues for their further structure–property investigation and potential applications.
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MICROCHIMICA ACTA
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2023
·Vol. 190
·期 10
·1-11
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IF 5.7
Tian Jiangman,Zhu Qihao,Huang Xiaojing,Li Yuan
A novel sandwich-type “on-off” electrochemiluminescence (ECL) biosensor for the determination of α(2,3)-sial-Gs was designed. Specifically, amino-functionalized porous silica nanoparticles (HPSNs-NH 2 ) were first prepared and then decorated with gold nanoparticles (Au NPs) to form HPSNs-NH 2 @Au NP nanocomposite, which exhibited a strong ability to enhance ECL intensity with K 2 S 2 O 8 as co-reactant (signal-on) and could immobilize the target-specific binding molecules of maackia amurensis lectin (MAL). Additionally, AuPdPt trimetallic nanoparticles were prepared to serve as a quenched ECL signal indicator (signal-off) with the ability of capturing the target non-specific binding molecules of 3-aminophenylboronic acid (APBA) to form a signal label. The sandwich-type ECL biosensor was constructed based on the structure of MAL-α(2,3)-sial-Gs-APBA and achieved a determination toward α(2,3)-sial-Gs with a wide linear range from 1 fg mL −1 to 10 ng mL −1 and a low detection limit of 0.5 fg mL −1 . Furthermore, the proposed ECL biosensor showed satisfactory selectivity, stability, and reproducibility for α(2,3)-sial-Gs determination. Graphical Abstract
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MOLECULES
·
2023
·Vol. 29
·期 1
·171
·
IF 4.6
Wenjie Li,Shenghui Hao,Hengyang Li,Qi An,Lina Yang,Bing Guo,Zijing Xue,Yongli Liu,Long Guo,Yuguang Zheng,Dan Zhang
Metabolic product accumulation exhibited variations among mulberry (Morus albaL.) leaves (MLs) at distinct growth stages, and this assessment was conducted using a combination of analytical techniques including high-performance liquid chromatography (HPLC), gas chromatography–mass spectrometry (GC-MS), and liquid chromatography–mass spectrometry (LC-MS). Multivariate analysis was applied to the data, and the findings were correlated with antioxidant activity and α-glucosidase inhibitory effects in vitro. Statistical analyses divided the 27 batches of MLs at different growth stages into three distinct groups. In vitro assays for antioxidant activity and α-glucosidase inhibition revealed that IC50values were highest at the Y23 stage, which corresponds to the ‘Frost Descends’ solar term. In summary, the results of this study indicate that MLs at different growth stages throughout the year can be categorized into three primary growth stages using traditional Chinese solar terms as reference points, based on the observed variations in metabolite content.Keywords:Morus albaL.;primary metabolites;secondary metabolites;different growth stages;antioxidant activity
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2023
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IF 9.9
Hongtong Hu,Yuyan Chang,Zhongjie Wang,Jiandong Cui,Shiru Jia,Yingjie Du
The combination of chemo- and biocatalysts to perform one-pot synthetic route has presented great challenges for decades. Herein, glutamate oxidase (GLOX) and trimanganese tetraoxide (Mn 3 O 4 ) nanocrystals were combined for the first time by one-step biomineralization to construct a mimic multi-enzyme system ( [email protected] 3 O 4 ) for chemoenzymatic synthesis of α‑ketoglutaric acid (α‑KG). Mn 3 O 4 not only served as a support for the enzyme immobilization, but also contributed its catalytic activity to co-operate with natural enzymes for the cascade reactions . The as-synthesized chemo-enzyme catalysts with directly contacted catalytic sites of the enzyme and inorganic catalyst maximizes the substrate channeling effffects for in situ rapid decomposition of the oxidative intermediate, H 2 O 2 , during the enzymatic oxidation of sodium glutamate, thus relieving the inhibition of H 2 O 2 accumulation for GLOX. Benefiting from the excellent stability and reusability of [email protected] 3 O 4 , a nearly 100% conversion (99.7%) of l -glutamate to α-KG was achieved, over 4.7 times higher than that of the free GLOX system (21.2%). This work provides a feasibility for constructing a high-performance chemo-enzyme catalyst for cascade catalysis, especially for those reactions with toxic intermediates.
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JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
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2023
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IF 5.7
Binwei Huang,Pingzhan Si,Quanxing Ren,Zhuang Qian,Ziqiang Yin,Zhengren Huang,Qing Huang,Yinsheng Li
SiC ceramic was fabricated by spark plasma sintering of β-SiC powder and Y 2 O 3 -MgO additives in argon. The effects of β→α phase transformation of SiC on microstructure and thermal conductivity of densified bulks were systematically investigated, in comparison to the counterparts using α-SiC as starting powder. The β→α phase transformation led to a “unimodal to bimodal” transition in grain size distribution. After sintering at 1850 o C, the incomplete β→α phase transformation induced the appearance of β/α heterophase boundary with strong effect of phonon-scattering. After sintering at 2050 o C, the completion of β→α phase transformation resulted in enlarged grains and disappearance of β/α heterophase boundary in SiC ceramic. The lattice oxygen content was decreased primarily by enhanced grain growth and oxygen picking-up of sintering additives, and possibly some contribution from β→α phase transformation. The optimized microstructure enabled SiC ceramic to obtain a remarkable increase in thermal conductivity from 126 to 204 W/mK after the replacement of α-SiC by β-SiC as starting powder and the accomplishment of β→α phase transformation.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2023
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IF 8.6
Xinyi Chen,Yunlong Wu,Ru Feng,Kai Yu,Jianming Pan
Hollow polymer microspheres (MPs) have attracted much attention in the field of separation science and technology due to their low density, high specific surface area, easy chemical modification, and abundant binding sites. However, traditional methods for preparing hollow microspheres typically involve surfactants and expensive templates, which are time-consuming and uneconomical, and are not suitable for large-scale production. Oil-soluble α-ethyl cyanoacrylate (ECA), a highly reactive monomer, has been employed to prepare hollow microspheres efficiently in the current study. Self-stabilizing encapsulation via fast interfacial polymerization of ECA would confer a special Pickering-like emulsion droplet reactor for rapid microcapsule formation. The ECA acts as both a monomer and a Pickering emulsifier during the reaction, in which case the newly formed polyECA (PECA) nanoparticles accumulate at the interface, forming a shell of PECA layer eventually. Excellent mechanical property of the PECA layers prevent droplets from coalescence, resulting in hollow PECA MPs after drying. To provide adsorption sites for chelating of uranyl ions, a water-soluble vinyl monomer grafted with ethylene phosphate (vinylphosphonic acid) was then introduced onto the non-reactive double bonds of the polyECA chain under mild reaction conditions, owning to the slightly amphiphilic nature of the PECA chain. The obtained adsorbent for uranium extraction is thus named as PECA-PO 4 3− MPs. Owing to the accessible affinity sites and hollow structure, PECA-PO 4 3− MPs displayed high adsorption capacities at pH = 7–9, showing remarkable selectivity for uranium even in the presence of various competition ions. Moreover, PECA-PO 4 3− MPs exhibited high stability as well as excellent recyclability toward uranium capture after five regeneration cycles. Therefore, this work not only presents a new clue to fabricate novel functional MPs in a high-efficiency way, but also provides a novel platform for efficient uranium extraction.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Qianqian Feng,Wei Yang,Zhiyun Peng,Guangcheng Wang
Oxalis corniculate L. ( O. corniculate ) was used to treat diabetes in Chinese folk as a popular tea drink. In this work, 31 compounds from O. corniculate were screened and identified as potential α-Glucosidase inhibitors (α-GIs). Among them, 6 compounds displayed stronger inhibitory activity than acarbose (IC 50 = 212.9 ± 5.98 μg/mL). Especially, the most effective compounds quercetin (Qu, IC 50 = 4.70 ± 0.40 μg/mL) and luteolin (Lu, IC 50 = 15.72 ± 0.75 μg/mL) inhibited α-Glu in competitive and mixed manners, respectively. Moreover, fluorescence quenching , circular dichroism (CD), and molecular docking study revealed that they can arouse the changes in the secondary structure and hydrophobic micro-environment of the enzyme mainly through a hydrophobic binding. Furthermore, it was observed that oral administration of Qu (20 mg/kg) can significantly reduce postprandial blood glucose (PBG) levels in mice vs. the control group. To sum up, the above research confirmed that O. corniculate could prevent and treat postprandial hyperglycemia as a good tea drink, and the plant was an excellent source to obtain natural α-GIs.
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危险品化学品经营许可证（带存储）