MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 6
·1-10
·
IF 5.479
Luo Zibin,Sun Duanping,Tong Yanli,Zhong Yongsheng,Chen Zuanguang
An ultrasensitive voltammetric aptasensor was constructed to analyze cardiac troponin I (cTnI). It is based on DNA nanotetrahedron (NTH) linked dual-aptamer (dAPT) and magnetic metal organic frameworks (mMOFs) of type Fe 3 O 4 @UiO-66. Firstly, the DNA NTH linked dAPT (Tro4 and Tro6) were immobilized on a gold electrode for improving the capture efficiency of cTnI. The novel mMOFs Fe 3 O 4 @UiO-66 was then decorated by Au@Pt nanoparticles (Au@PtNPs), horseradish peroxidase (HRP), G-quadruplex/hemin (GQH) DNAzyme, and two types of aptamers to form signaling nanoprobes. In the presence of cTnI, an aptamer-protein-nanoprobe sandwich-type structure is formed. Afterward, the nanoprobes including enzyme, GQH DNAzyme and Fe 3 O 4 @UiO-66/Au@PtNP were utilized to catalyze the oxidation of hydroquinone by hydrogen peroxide for the electrochemical signals amplification, typically at a working potential of −0.1 V (vs. Ag/AgCl). The voltammetric signal increases linearly in the 0.01 to 100 ng·mL −1 cTnI concentration range, and the detection limit is 5.7 pg·mL −1 .
An ultrasensitive voltammetric aptasensor was constructed to analyze cardiac troponin I (cTnI) based on DNA nanotetrahedron linked dual-aptamer and magnetic metal organic frameworks of type Fe 3 O 4 @UiO-66. The results indicated the aptasensor has a wide linear response range (0.01 to 100 ng/mL) and low detection limit (5.74 pg/mL) for cTnI. GE: gold electrode; MCH: 6-Mmercapto-1-hexanol; HRP: horseradish peroxidase; HQ: hydroquinone; BQ: benzoquinone.
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FASEB JOURNAL
·
2022
·Vol. 36
·期 5
·e22266
·
IF 5.834
Huiqing Hu,Ruxing Zhao,Qin He,Chen Cui,Jia Song,Xinghong Guo,Nan Zang,Mengmeng Yang,Ying Zou,Jingwen Yang,Jinquan Li,Liming Wang,Longqing Xia,Lingshu Wang,Falian He,Xinguo Hou,Fei Yan,Li Chen
Type 2 diabetes mellitus (T2DM) is an age-related disease characterized by impaired pancreatic β cell function and insulin resistance. Recent studies have shown that the accumulation of senescent β cells under metabolic stress conditions leads to the progression of T2DM, while senolysis can improve the prognosis. However, the specific mechanism of β cell senescence is still unclear. In this study, we found that the increased load of senescence pancreatic β cells in both older mice and obese mice induced by high-fat diet (HFD) (DIO mice) was accompanied by activation of the Cyclic GMP-AMP synthase (cGAS) - stimulator of interferon genes (STING) pathway and using cGAS or STING small interfering RNA or STING inhibitor C176 to downregulate this pathway reduced the senescence-associated secretion profile (SASP) and senescence of Min6 cells treated with palmitic acid or hydrogen peroxide. C176 intervention in DIO mice also significantly reduced the inflammation and senescence of the islets, thereby protecting the function of pancreatic β cell and glucose metabolism. Our study further revealed that mitochondrial DNA (mtDNA) leakage under metabolic stress conditions was critical for the activation of the cGAS-STING pathway, which can be reversed by the mtDNA depleting agent ethidium bromide. Consistently, mtDNA leakage was more severe in older mice and was accelerated by a chronic HFD. In conclusion, we demonstrate that cytoplasmic mtDNA activates the cGAS-STING pathway to mediate SASP during the accelerated senescence of pancreatic β-cells induced by metabolic stress, and this process can be downregulated by the STING inhibitor C176.
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 2
·1-9
·
IF 6.232
Li Mei,Xu Xiong,Zhou ZhongGao,Xu GuoHai,Xie YongRong,Cai QingYou
A method is described for the determination of microRNAs via two-stage signal enhancement. This is attained by combining hairpin (HP) assisted cascade isothermal amplification with light-up DNA-Ag nanoclusters. A rationally designed dual-functional HP is used, and microRNA-21 is chosen as a model analyte. At the first stage, upon the hybridization of the microRNA-21 with HP, microRNA recycling via polymerase-displacement reaction and a circulative nicking-replication process are achieved. This generates numerous G-abundant overhang DNA sequences. In the second stage, the above-released G-abundant overhang DNA sequences hybridize with the dark green Ag NCs, and this results in the appearance of bright red fluorescence. Thanks to the two signal enhancement processes, a linear dependence between the fluorescence intensity at 616 nm and the concentration of microRNA-21 is obtained in the range from 1 pM to 20 pM with a detection limit of 0.7 pM. The strategy clearly discriminates between perfectly-matched and mismatched targets. The method was applied to the determination of microRNA-21 in a spiked serum sample.
Schematic representation of microRNA detection by integrating hairpin assisted cascade isothermal amplification with light-up DNA Ag nanoclusters. With microRNA, G-abundant overhang DNA sequences from amplification reaction hybridize with dark green Ag nanoclusters to produce a concentration-dependent bright red fluorescence
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Scientific Reports
·
2016
·Vol. 6
·期 1
·1-8
·
IF 5.228
Lan Jianming,Liu Yingxin,Li Li,Wen Fadi,Wu Fang,Han Zhizhong,Sun Weiming,Li Chunyan,Chen Jinghua
High-sensitivity detection of trace amounts of c-erbB-2 oncogene was reported to be equal to or surpass the ability of CA 15-3 for early diagnosis and/or follow-up recurrent screening of breast cancer. Therefore, in the current study, by using upconversion nanoparticles (UCNPs), rare earth-doped NaYF 4 :Yb 3+ /Er 3+ as the luminescent labels, a upconversion luminescent (UCL) biosensor based on dual-signal amplification of exonuclease III (ExoIII)-assisted target cycles and long-range self-assembly DNA concatamers was developed for the detection of c-erbB-2 oncogene. The proposed biosensor exhibited ultrasensitive detection with limit as low as 40 aM, which may express the potential of being used in trace analysis of c-erbB-2 oncogene and early diagnosis of breast cancer.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Ping-Ping He,Xinyu Wang,Zhaosheng Fan,Kunpeng Ma,Zhaoyan Tian,Zhe Wang,Enxiang Zhang
Effective regeneration of injured skeletal muscle remains a major challenge in clinical medicine. There is an urgent demand for therapeutic strategies capable of achieving precise spatiotemporal control over the muscle repair process. To address this clinical challenge, we propose an ATP-responsive injectable DNA hybrid hydrogel, specifically designed to enable “on-demand release” of cytokines, thereby enhancing muscle regeneration. Through the implementation of rational DNA sequence design and optimized cross-linking methodologies, the three-dimensional network of the hydrogel, which is capable of efficiently encapsulating a diverse range of cytokines known to promote tissue repair and remodeling, was constructed. This hydrogel can respond to the characteristic increases in ATP concentration fluctuations within the local tissue microenvironment, enabling precise control over cytokine release and thereby promoting the proliferation and differentiation of muscle cells. This DNA hydrogel, exhibiting excellent biocompatibility, creates a more favorable and functionally optimized microenvironment that supports and promotes muscle regeneration. This “on-demand release” capability not only enhances therapeutic efficacy but also prevents premature drug release prior to reaching the target site and minimizes off-target drug distribution to non-target tissues, thereby improving both the safety and controllability of regenerative treatments.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 9
·595
·
IF 5.4
Li Jing,Jin Xiaojuan,Lin Tongze,Chang Yajuan,Liu Sunan,Wang Xu,Shi Ruien,Sheng Quanbo,Zheng Maokun,Zhu Ling,Ma Yunsu
A lateral flow analysis (LFA) strip for detecting dual miRNAs (miR155 and miR21) was constructed based on the “AND” logic gate and DNA self-assembly. The ternary synergistic strategy of “logic gating-signal amplification-visual output” enables highly sensitive and visual detection of miR21 and miR155 for breast cancer diagnosis. Only if both miR21 and miR155 are present can the trigger DNA (TDNA) in the sensor specifically dissociate and activate the “AND” logic gate (output signal = “1”); in the absence of either miRNA, the system remains in an inactive state (output signal = “0”). TDNA initiates catalytic hairpin assembly (CHA), and the product of the CHA reaction cascaded triggers the hybridization chain reaction (HCR) to achieve dual signal amplification. Gold nanoparticles (AuNPs) were used to label DNA for the construction of the LFA strip, allowing naked-eye observation of the detection results of miR21 and miR155. Detection results showed that the signal intensity of the test line (T line) had a positive correlation with the concentration of miRNAs (with a miR155:miR21 ratio of 1:1). Within the concentration range of 0.1–10 nM, the average grayscale value of the T line exhibited a good correlation with the logarithm of miRNA concentration (correlation coefficient r = 0.9983), and the limit of detection (LOD) was 30.98 pM. This LFA strip has been preliminarily validated to the detection of human serum samples, and the results indicated that the levels of miR155 and miR21 in the serum of breast cancer patients were significantly higher than those in normal individuals. Free from the limitation of large-scale instruments, this strip holds great commercialization potential. Graphical abstract
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ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Zhaoying Liu,Yuan Kong,Kan Yang,Guangyun Chen,Ming Li
Background The overuse of ampicillin (AMP), a pivotal antibiotic, poses severe environmental and ecological risks, necessitating portable and precise monitoring. Results A highly integrated Lab-in-Tube biosensing platform based on Ag + -coupled hairpin (Ag@hairpin) aptamer and gold nanoparticles-immobilized DNA (AuNP@DNA) hydrogel for the portable and ultrasensitive detection of AMP was developed. In this pre-assembled tube platform, Ag@hairpin aptamers and AuNP@DNA hydrogels were immobilized on the inner bottom and cap of the tube, respectively. Upon introduction of AMP, the released Ag + triggered Ag-DNAzyme to cleave S-DNA, generating abundant AuNP. These AuNP were subsequently introduced into a glucose/FeNi-doped polydopamine nanozyme (FeNi@PDA) system to generate ultrasensitive colorimetric signals. The biosensing requires only a simple two-step upside down operation in the sealed tube, achieving an excellent limit of detection (LOD) of 1.02 pg/mL. The platform demonstrated high specificity (cross-reactivity less than 4.31%) and practical applicability in environmental samples. Furthermore, the advantages of miniaturized platform, simplified operation and portability had demonstrated. Significance By integrating accurate biosensing with an ultrasensitive enzyme cascade system, this Lab-in-Tube platform may offer an advanced strategy for the portable and on-site detection of other targets.
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ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Runzi Zhang,Shunbi Xie,Xiaoyu Yang,Wanyi Xiong,Yi He
Currently, surface-enhanced Raman scattering (SERS) biosensors face practical challenges, including SERS substrate waste and unsatisfactory detection results. Accordingly, the highly efficient and regenerable SERS substrate HOF@Au was collaborated with a target-triggered identifying-cleaving-rolling-assembly cycling nanodevice (ICRACN) as a cascade signal amplification system to achieve an ultrasensitive detection of roxithromycin (RXM). In this system, abundant Au nanoparticles (NPs) were reduced in situ on the surface of alkali-resistant HOF-102 to form a uniform and high-enhancement SERS substrate HOF@Au, thereby enhancing the accuracy and stability of the sensor. Furthermore, aptamer-mediated specific recognition, efficient cleavage activity of DNA nanorollers, and a pH-responsive DNA triplex structure were engineered to construct ICRACN. In the presence of the target, it was specifically recognized by the aptamer, leading to the activation of the DNAzyme. Meanwhile, the dynamic nanorollers were triggered to initiate cleavage of a specific site along a defined pathway, enabling highly efficient signal amplification. Concurrently, the triplex structure formed by the two single-stranded DNA atoms on the SERS substrate was opened, allowing the capture of the SERS tag and generating a pronounced SERS signal. The proposed strategy achieved the sensitive detection of RXM with a low limit of detection of 3.97 × 10–14 mol/L. Moreover, modulation of pH induced reversible folding and unfolding of the triplex structure to facilitate regeneration of the SERS substrate, which simplified operational procedures and reduced detection costs. As a result, this work presents a novel, regenerable SERS biosensor and offers valuable insights into the design of multifunctional sensing platforms for analytical applications.
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ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Junli Liu,Zhijuan Duan,Xianyan Gong,Fan Xia,Fujian Huang
Abstract Apurinic/apyrimidinic endonuclease 1 (APE1) is a clinical biomarker whose small molecular size hinders direct detection by conventional nanopores (30 nm). Here, we report a nanopipette platform integrating APE1-driven enzymatic recycling amplification with DNA tetrahedron−streptavidin (TDN−SA) complex-based resistive-pulse reporting to overcome this size limitation. APE1 specifically cleaves hairpin DNA probes, generating output strands that displace biotin-modified DNA oligo from the TDN−SA complex, reducing translocation event frequency in 30 nm nanopipettes. This signal modulation enables APE1 quantification with a detection limit of 10−5 U/mL and a linear range spanning 7 orders of magnitude (10−5 to 10 U/mL). The method discriminates APE1 activity between normal and cancer cell lines (HeLa, MCF-7, A549 vs. 293T, MCF-10A), tracks oxidative stress-induced elevation (0−4 h H2O2 treatment), and reveals cell cycle-dependent regulation (lowest in G0/G1, peaking in S phase). Furthermore, the system evaluates APE1 inhibition by 7-nitroindole-2-carboxylic acid in inflammatory macrophages, demonstrating its utility for drug efficacy assessment. This label-free platform overcomes the protein size constraint of traditional nanopore methods, offering a versatile tool for profiling low-abundance protein biomarkers in native cellular contexts.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2026
·
IF 6
Zhidi He,Yulin Xu,Zhipeng Tian,Dong Chen,Ziyan Dong,Shiyu Zhu,Man Li,Rong Guo,Bing Xiang,Qin He
Despite its clinical utility, the therapeutic potential of Venetoclax (ABT-199) in acute myeloid leukemia (AML) remains limited by the heterogeneous nature of the disease, which often leads to reduced efficacy of monotherapy. Herein, we demonstrate that ABT-199-induced apoptosis can be potentiated by all-trans retinoic acid (ATRA) to suppress the DNA damage response. To address the lack of targeted drug delivery against AML and facilitate enhanced therapeutic efficacy, we engineered a biomimetic micelle, HPAA, by co-assembling an amphiphilic mPEG-ATRA conjugate with ABT-199 into micelles, followed by cloaking with P-selectin-rich membranes from activated human umbilical vein endothelial cells to target CD44 overexpressed on AML cells. The resulting HPAA micelles exhibited enhanced cellular uptake in MOLM13 cells, achieving approximately 2-fold higher uptake efficiency than non-targeted PAA micelles. Consequently, HPAA induced robust apoptosis and superior anti-leukemic effects, significantly suppressing MOLM13 cell proliferation in vitro. In an orthotopic AML model, HPAA preferentially accumulated in the bone marrow resident AML cells. Accordingly, treatment substantially reduced the MOLM13 cell burden in the peripheral blood, bone marrow and liver. This work establishes HPAA as a promising biomimetic nanoplatform for AML therapy by integrating active targeting with the novel drug repurposing of ATRA as a Chk1 suppressor to potentiate ABT-199 efficacy. HPAA potentiates ABT-199 efficacy in acute myeloid leukemia by repurposing ATRA to suppress Chk1, thereby inducing significant apoptosis. Download: Download high-res image (110KB) Download: Download full-size image
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JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Kun Fu,Jia Zheng,Yichao Zhang,Chang Liu,Wenxiang Zhang,Siyu Chen
Current analytical methodologies for phthalic acid esters (PAEs) face significant challenges in achieving rapid on-site detection and the discriminative identification of individual congeners. This study utilizes Capture-SELEX technology to identify aptamers specifically recognizing dibutyl phthalate (DBP), di-n-octyl phthalate (DNOP), and diethyl phthalate (DEP). Fluorescence-based affinity assays revealed that these aptamers possess dissociation constants (Kd) of 1.577, 0.889, and 0.173 μM for their respective targets. Molecular docking simulations, coupled with base mutation analyses, were conducted to predict potential binding interfaces. By integrating these aptamers with the previously characterized di(2-ethylhexyl) phthalate (DEHP)-aptamer, a gold nanoparticle (AuNPs)-mediated visual detection platform was developed to validate the efficacy and potential of these aptamers for subsequent applications. The calculated limits of detection (LOD) for DBP, DEHP, DNOP, and DEP using this system are 17.64, 2.32, 25.68 and 22.01 ng/mL, respectively. Furthermore, a lateral flow strip was designed to enable the simultaneous and on-site multiplex detection of the four PAEs. This strip achieved LODs of 12.54, 14.95, 14.93 and 30.28 ng/mL for DBP, DEHP, DNOP, and DEP, respectively. A comparative evaluation using LC–MS on identical samples revealed quantitative concordance within a ratio range of 94.18 %–110.43 %, highlighting the robust analytical performance, detection efficiency, and translational potential of the strip for widespread application in various PAE testing scenarios.
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ACS Nano
·
2025
·
IF 16
Xiaohui Zhan,Yujia Jiang,Zhiyu Li,Xiaoqin Hu,Fang Lan,Binwu Ying,Yao Wu
The screening and monitoring of microRNAs as cancer molecular biomarkers is clinically significant, but traditional methods lack sufficient sensitivity, accuracy, and convenience. The CRISPR-colorimetric lateral flow assay (CLFA) integration offers a promising and efficient solution; however, cumbersome preamplification and poor quantification hinder clinical adoption. In this study, we developed a one-step isothermal CRISPR-Cas cascaded sensing system that is preamplification-free. At its core is a designed and selected split DNA tetrahedron activator, employing spatiotemporal-hierarchy mechanisms to precisely bidirectionally drive the kinetics of two Cas enzymes, accelerating the activation of Cas13a while delaying the initiation of Cas12a, to achieve optimal balance. This system enables ultrasensitive, single-step, single-tube, and rapid detection of a cervical cancer relative biomarker, microRNA-21, achieving a limit of detection of 38 aM with a broad linear range. The CRISPR system is further integrated with CLFA enhanced by ultrathin platinum-protected gold nanolabels (Au@Pt, also named Au@s-Pt), along with a smartphone equipped with dual convolutional neural network models (YOLO v5 and MobileNet v3), enabling more precise, rapid quantification of target miRNA. Using this integrated platform, miRNA-21 levels in cervical cancer and precancerous samples can be accurately quantified with approximately 30 min at low cost and without the need for large, sophisticated instruments, with results showing good concordance with quantitative real-time polymerase chain reaction. This platform provides an efficient, highly sensitive, user-friendly, and quantifiable point-of-care testing solution.
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