CARBOHYDRATE POLYMERS
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2024
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IF 10.7
Jingmei Teng,Wei Zhao,Shengyu Zhang,Dan Yang,Yu Liu,Rongjian Huang,Yuxi Ma,Lei Jiang,Hua Wei,Jiantao Zhang,Jing Chen
Skin wounds are susceptible to infection, leading to severe inflammatory reactions that can progress to chronic wounds, ultimately causing significant physical and mental distress to the patient. In this study, we propose an injectable composite hydrogel achieved through one-pot gelation of oxidized xyloglucan (OXG), cationic polyamide ε-Poly- l -lysine (EPL), and surface amino-rich silicon nanoparticles (SiNPs). OXG exhibits commendable anti-inflammatory properties and provides crosslinking sites. SiNPs serve as mechanically reinforced crosslinkers, facilitating the construction of a dynamic Schiff base network. SiNPs significantly reduced the gelation time to 3 s and tripled the storage modulus of the hydrogels. Additionally, the combination of EPL and SiNPs demonstrated synergistic antimicrobial activity against both S. aureus and E. coli . Notably, the hydrogel effectively halted liver bleeding within 30 s. The hydrogel demonstrated outstanding shear-thinning and self-healing properties, crucial considerations for the design of injectable hydrogels. Furthermore, its efficacy was evaluated as a wound dressing in a mouse model with S. aureus infection. The results indicated that, compared to commercial products, the hydrogel exhibited a shorter wound healing time, decreased inflammation, thinner epithelium, increased hair follicles, enhanced neovascularization, and more substantial collagen deposition. These findings strongly suggest the promising potential of the proposed hydrogel as an effective wound dressing for the treatment of infected wounds.
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Food Bioscience
·
2025
·
IF 5.9
Xueyi Cai,Hongfu Li,Mengzhen Liao,Qihong Zheng,Huan Chen,Yang Zhang,Xiao Chen,Dong Liang
Bitter gourd (fruit of Momordica charantia L.) is a popular vegetable and medical herb known for its intense bitter taste. However, its major bitter-tasting compounds have not been fully characterized. This study aimed to decode its bitterness by application of the sensomics approach. Combining solvent extraction, sensory analysis-guided fractionation and purification with medium-pressure liquid chromatography and high-pressure liquid chromatography, nine bitter-tasting cucurbitane-type triterpenoids were isolated from bitter gourd. The structures of isolated compounds were determined by high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy. The bitter taste recognition thresholds of isolated compounds were in the range from 1.4 mg/L ((23 E )-3 β - O -malonyl-7 β ,25-dihydroxycucurbita-5,23-dien-19-al, 9 ) to 19.1 mg/L (yeojooside C, 5 ) in 3% ethanol as determined by trained human assessors. Quantification with UPLC-MS/MS demonstrated their natural concentrations in bitter gourd fruits were in the range between 14.2 ± 0.9 mg/kg (yeojooside C, 5 ) and 245.6 ± 22.1 mg/kg (3- O -malonylmomordicine I, 10 ) on a dry weight basis. Calculation of the dose-over-threshold (DoT) factors showed three compounds, namely 3- O -malonylmomordicine I ( 10 ), momordicine I ( 3 ), and (23 E )-3 β - O -malonyl-7 β ,25-dihydroxycucurbita-5,23-dien-19-al ( 9 ) were the bitter principles in bitter gourd fruits. On the other hand, momordicoside K ( 2 ), which had been known as a bitter principle of bitter gourd for more than forty years, had been proven to be an extraction and isolation artifact rather than a natural component of the plant. Besides, an acid-catalyzed isomerization reaction in the side chain of cucurbitane was discovered, which might explain the formation mechanism of many cucurbitane-type triterpenoids from bitter gourd.
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Journal of Environmental Chemical Engineering
·
2025
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IF 7.2
Xinyi Liu,Huiyan Wei,Meiqi Ju,Chunying Pang,Dandan Wang,Yuming Liu,Shuhua Zhang,Hongji Li
Tryptophan is a potential biomarker for a variety of diseases, so rapid and accurate detection of tryptophan levels in the human body is essential. In this work, surface-enhanced Raman scattering (SERS) detection was combined with acid-catalyzed silicon-based molecularly imprinted polymers to synthesize Cu 2 O@Ag-MIPs (CA-MIPs) for selective detection of L-tryptophan(L-Trp). Compared with traditional detection methods, SERS detection has the advantages of being sensitive, fast and nondestructive. Here, Ag nanoparticles are uniformly loaded on {100} surface-dominated Cu 2 O sub-microcubes to excite strong localized surface plasmon resonance, which exhibits good SERS performance. Considering the complexity of practical detection, an acid-catalyzed method was adopted to load silicon-based imprinted polymers with high biocompatibility on the Cu 2 O@Ag surface, which improved the drawbacks of the common silicon-based imprinting method, such as loose structure and long reaction time. The thickness of the imprinted layer can be controlled to ensure selectivity while ensuring superior SERS sensitivity. The prepared CA-MIPs were shown good linearity with L-Trp concentration in the range of 10 −3 –10 −10 mol L −1 , and the sensor also exhibited excellent selectivity for the determination of L-Trp in the presence of other interfering amino acids. In addition, the method was shown good recoveries in spiked recovery tests of simulated urine samples and was expected to be practically applied to the detection of clinical disease markers.
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Journal of Environmental Chemical Engineering
·
2024
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IF 7.4
Lei Sun,Wei Zhang,Yakun Feng,Zequn Fan,Lei Jiang,Can Lu
Biodegradable microplastics (BMPs) and cadmium (Cd) present substantial risks to agricultural systems, primarily affecting plants. Most current studies have focused on the ecological effects of virgin BMPs. However, the effects of ultraviolet (UV)-aged BMPs and their interactions with cadmium (Cd) on pakchoi remain largely unknown. Therefore, this study addressed this gap by aging polylactic acid (PLA) microplastics (MPs) through UV irradiation and conducting an indoor hydroponic experiment. The research systematically examined the individual and combined impacts of Cd, virgin, and UV-aged BMPs on pakchoi ( Brassica chinensis L. ), focusing on plant growth, photosynthesis, antioxidant systems, and Cd accumulation. The results showed that after 21 days of UV irradiation (UPLA MPs), UPLA MPs with a fractured surface had a more significant impact on pakchoi than untreated PLA MPs. Cd alone inhibited pakchoi growth, development, and photosynthesis, while PLA/UPLA MPs alone promoted these processes. Under the combination of Cd and PLA/UPLA MPs, UPLA MPs could effectively alleviate the toxic effect of Cd on pakchoi. Besides, Cd coexisted with PLA/UPLA MPs, and with the increase of Cd concentration, UPLA MPs(41.22 %, 66.80 %, 45.57 %) decreased the Cd content of pakchoi more significantly than PLA MPs(37.87 %, 55.91 %, 26.35 %). Meanwhile, the results of Partial least squares structural equation models (PLS-SEM) further showed that UPLA MPs could effectively reduce the accumulation of Cd in plants. Therefore, the results of this study can provide a new perspective and reference for the ecological risk evaluation of Cd and BMPs pollution in agricultural soils.
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MOLECULES
·
2024
·Vol. 29
·期 12
·2856
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IF 4.2
Qin Huang,Hua Zhou,Xianglun Wu,Jiaqi Jiang,Bingdong Zhan,Pinggu Wu
A promising method was established for the determination of nine halobenzoquinones (HBQs) in potable water by membrane solid-phase extraction (MSPE) pretreatment and the liquid chromatography–mass spectrometry (LC-MS) method. A 500 mL water sample was taken for enrichment by the SDB-RPS membrane, which was previously activated by methanol and ultrapure water. The sample was eluted with methanol and re-dissolved with the initial mobile phase after nitrogen blowing. Then, it was detected in negative ion mode using the working curve, and HBQs were quantified by the external standard method. The linearity was satisfactory in the concentration range of 4-1000 ng/L, with correlation coefficients of 0.9963~0.9994. The recoveries were 73.5~126.6% at three spiked levels, with relative standard deviations (RSDs) of 6.8~15.5%. The limits of detection (LOD, S/N = 3) values were 0.1~0.7 ng/L. The results demonstrate that the MSPE-LC-MS method is reliable, rapid, and sensitive for the simultaneous analysis of nine HBPs in potable water.
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JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
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2020
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IF 2.69
Yasong Hu,Congcong Zhan,Anduo Zhou,Shanfeng Zhang,Juying Chen,Xia Huang
A novel amino acid-modified biomacromolecule was designed and synthesized as the quaternary ammonium salt chitosan grafted-tyrosine (CA-g-Tyr) suitable for biomedical applications. L-tyrosine was grafted onto the quaternary ammonium salt chitosan (CA) by N-(3-dimethylaminopropy)-N-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). The chemical structure of CA-g-Tyr was confirmed by Fourier transform infrared (FTIR) spectroscopy and 13C-NMR. The change in the crystalline structure after the graft was characterized by differential scanning calorimetry (DSC) and powder X-ray diffraction (PXRD). The surface wettability and moisturizing performance of the CA-g-Tyr were also characterized. The CA-g-Tyr film possessed good hydrophilicity, and the mechanical tensile experiments showed that the introduction of tyrosine gave CA mechanical properties more suitable for blood vessel. Cell experiments showed that the endothelial cells can adhere and proliferate better on the surface of a CA-g-Tyr film than CA. The results confirm the favorable properties and biocompatibility of CA–g-Tyr with potential applications as scaffolds for tissue engineering.
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BIOCHEMICAL PHARMACOLOGY
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2024
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IF 5.3
Yufeng Dai,Jinxiang Chen,Jialong Fang,Shuxiao Liang,Hao Zhang,Haitao Li,Wei Chen
Metabolic dysfunction-associated fatty liver disease (MAFLD) encompasses a broad spectrum of hepatic disorders, including hyperglycemia, hepatic steatosis, and insulin resistance. Piperlongumine (PL), a natural amide alkaloid extracted from the fruits of Piper longum L., exhibited hepatoprotective effects in zebrafish and liver injury mice. This study aimed to investigate the therapeutic potential of PL on MAFLD and its underlying mechanisms. The findings demonstrate that PL effectively combats MAFLD induced by a high-fat diet (HFD) and improves metabolic characteristics in mice. Additionally, our results suggest that the anti-MAFLD effect of PL is attributed to the suppression of excessive hepatic gluconeogenesis, inhibition of de novo lipogenesis, and alleviation of insulin resistance. Importantly, the results indicate that, on the one hand, the hypoglycemic effect of PL is closely associated with CREB-regulated transcriptional coactivators (CRTC2)-dependent cyclic AMP response element binding protein (CREB) phosphorylation; on the other hand, the lipid-lowering effect of PL is attributed to reducing the nuclear localization of sterol regulatory element-binding proteins 1c (Srebp-1c). Mechanistically, PL could alleviate insulin resistance induced by endoplasmic reticulum stress by antagonizing the thromboxane A 2 receptor (TP)/Ca 2+ signaling, and the TP receptor serves as the potential target for PL in the treatment of MAFLD. Therefore, our results suggested PL effectively improved the major hallmarks of MAFLD induced by HFD, highlighting a potential therapeutic strategy for MAFLD.
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ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Jiahui Gong,Mengfei Tian,Ying Liu,Yuting Zhang,Junhui Xing,Qi Liang,Xiaofei Xie,Chunying Li,Chunjian Zhao
In this work, a dual-responsive ionic liquid (DR-IL), namely, [C4mim][FeCl4] was developed for simultaneously extracting and separating hydrophilic phytochemicals, including epicatechin (EC), dihydroquercetin (DQ), ferulic acid (FA), and hydrophobic phytochemicals, such as emodin (ED), chrysophanol (CP), and physcion (PC), from roots of wild Rumex acetosa L. The [C4mim][FeCl4] demonstrates considerable potential for extracting and separating target phytochemicals through temperature adjustment and accelerated phase separation facilitated by a neodymium magnet. Meanwhile, a high recovery yield is obtained by adding a magnetic field. The overall extraction yield of target phytochemicals achieved by [C4mim][FeCl4] reaches 16.12 mg/g under the optimal extraction conditions, representing a 1.53–2.39-fold increase compared to conventional solvents. Furthermore, the molecular simulation and FT-IR analysis reveal that the synergistic effect of van der Waals forces and hydrogen bonds significantly contributed to the higher extraction yields of target phytochemicals.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Xueyi Cai,Ziqiang Lin,Qihong Zheng,Mengzhen Liao,Hongfu Li,Hanxiao Feng,Huan Chen,Yang Zhang,Xiao Chen,Dong Liang
The unripe fruit of the plant Momordica charantia L., commonly known as bitter gourd or bitter melon, is a popular vegetable and medical herb in many parts of the world and is characterized by its strong bitter taste. In our endeavor to decode its bitter taste, the dichloromethane fraction of 75% methanol extract of bitter gourd was found to be intensely bitter. Combining sensory analysis-guided fractionation and newly developed comparative high-pressure liquid chromatography (HPLC) analysis-guided purification led to the isolation of five known compounds including momordicoside L (1), (23E)-3β-O-malonyl-7β,25-dihydroxycucurbita-5,23-dien-19-al (2), 3-O-β-d-allopyranosyl-7β,25-dihydroxycucurbita-5,23(E)-dien-19-al (3), momordicine IV (5), and charantoside B (6) and three new compounds 3-O-β-d-3-ketoglucopyranosly-7β,25-dihydroxycucurbita-5,23(E)-dien-19-al (4), 6′-O-malonylmomordicoside L (7), and 6′-O-malonylmomordicine IV (8) from bitter gourd. Sensory analysis revealed compounds 3–8 had strong bitter taste with their bitter taste recognition thresholds in the range between 3.6 (4) and 13.6 ppm (6) in 3% ethanol. UPLC-MS/MS quantification showed that their concentration in bitter gourd ranged from 16.5 ± 1.3 mg/kg (4) to 214.8 ± 14.0 mg/kg (6) on a dry weight basis. Calculation of the dose-over-threshold (DoT) factor showed that momordicine IV (5) and charantoside B (6) should be considered as bitter principles of bitter gourd. In addition, the study also demonstrated the ubiquity of the isomerization reaction in the side chain of cucurbitane-type triterpenoids. Many isolated compounds were the isomerized products of their natural precursors, and these precursors should be the primary bitterness contributors of fresh fruits. In addition, comparative HPLC analysis-guided purification could be a practical approach for the fast isolation of acid-labile precursors.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Yue Huang,Xinyi Wang,Ying Lyu,Yu Li,Rongrong He,Haiming Chen
Non-enzymatic browning (NEB) reduced the colour quality of hot-air dried peppers, but the specific mechanism remains unclear. This may be related to the degradation of chlorophyll, ascorbic acid, and polyphenols. The findings revealed that the surface of pepper gradually browned during hot-air drying, with the Δ E ⁎ value and browning degree (BD) significantly increasing by 119.4 % and 62.9 %, respectively. However, the total phenol content (TPC) and ascorbic acid content decreased by 74.2 % and 84.3 %, respectively. TPC was negatively correlated with BD ( R = −0.86), and its value (31.7 %) was 300 times higher than that of other colour-related components (ascorbic acid, chlorophyll). UPLC-MS/MS analysis further identified 345 polyphenols, among which 1, 3-dicaffeoylquinic acid and 5,7-dihydroxy-3′, 4′, 5′-trimethoxyflavone were the two key monophenols influencing NEB ( R = −0.87). The results suggested that NEB pathways involve chlorophyll a nd ascorbic acid degradation, the Maillard reaction, and polyphenol degradation, with the latter playing a major role.
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ACS Applied Polymer Materials
·
2024
·
IF 4.4
Cijun Shuai,Xingming Long,Binxin Sun,Tiantian He,Xiong Shuai,Guoyong Wang,Shuping Peng
Bacterial infection is a severe challenge faced by artificial bone transplantation, which might cause delayed bone healing or even transplant failure. Photodynamic therapy (PDT) has garnered widespread attention as a treatment for infections due to its noninvasiveness, few side effects, and high spatiotemporal selectivity. Nevertheless, owing to the bacterial membrane obstacle, it is difficult for exogenous reactive oxygen species (ROS) to penetrate into bacteria, which leads to an unsatisfactory antibacterial effect. Herein, a heterojunction of Ti2C3 nanosheets/tin disulfide (MXene/SnS2) is designed, which integrates photothermal and photodynamic properties. Then, MXene/SnS2 was incorporated into a poly-l-lactic acid powder (PLLA) matrix to fabricate an artificial bone scaffold with selective laser sintering (SLS) technology. Under near-infrared laser irradiation, SnS2 can strengthen the near-infrared light absorption of MXene to generate local hyperthermia, thus enhancing bacterial membrane permeability. Meanwhile, MXene/SnS2 enhances charge transfer and inhibits electron–hole pair separation, thereby generating more ROS that can penetrate the bacterial interior. The results indicated that this antibacterial strategy has effective antibacterial activity, and the antibacterial rate reached over 90%. Overall, this research presents an attractive antibacterial strategy for implant-related infection.
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INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Xinru Li,Dongmei Wang,Bingjin Wang,Dengwu Li
Podophyllotoxin, along with its congeners and derivatives exhibit strong biological activity mainly as antitumor drugs and antiviral agents. Here, the ultrasound-assisted extraction of podophyllotoxins including α-peltatin, podophyllotoxin, podophyllotoxinone and deoxypodophyllotoxin from Juniperus sabina L. leaves using deep eutectic solvents (DESs) were developed first time. To simultaneously and effectively extract four target podophyllotoxins, 19 DESs were screened to determine the best solvent to extract podophyllotoxins. Choline chloride-lactic acid (1:1) with 30 % water content was proved to have the best extraction effect. The optimal extraction process with DES were DES/solid ratio 30 mL/g, reaction temperature 44℃, time 53 min and ultrasound power 495 W. Under optimized extraction conditions, the total podophyllotoxins extraction yield was 27.520 mg/g, 196.84 % higher than that using traditional methanol solvent. The yield of α-peltatin, podophyllotoxin, deoxypodophyllotoxin and podophyllotoxinone were 13.567, 9.548, 2.021, 2.204 mg/g, respectively. Subsequently, the podophyllotoxins were effectively isolated from the deep eutectic solvent using D101 macroporous resin. The optimal process for enriching target podophyllotoxins by D101 macroporous resin were as follows: 30 mg/mL of crude extract solution, five bed volumes feed volume for adsorption; six bed volumes of 80 % ethanol for desorption. Moreover, a scale-up enrichment experiment of podophyllotoxins was performed under optimum procedure, and the results showed that the target podophyllotoxins yield was increased by 8.92 times, with recoveries in the range of 75.86–85.69 %. Additionally, the enriched podophyllotoxins effectively restored the antibacterial efficacy of cefazolin against methicillin-resistant Staphylococcus aureus and colistin-resistant Escherichia coli , resulting in 16–64-fold reduction in MIC. Therefore, the method developed in this study could be an eco-friendly, efficient and sustainable alternative for the selective extraction and enrichment of active compounds from plant material.
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