JOURNAL OF PHARMACEUTICAL SCIENCES
·
2025
·
IF 3.8
Xiaoxuan Fei,Junyi Pei,Simin Yan,Weihong Ge,Li Li,Jianjun Zou,Huimin Zhang,Lie Sun,Sifei Han,Luojuan Hu
Compared to classic intravenous (IV) administration, subcutaneous (SC) delivery of monoclonal antibodies (mAbs) offers new opportunities to improve patient compliance and healthcare efficiency. However, insufficient understanding of drug absorption process following SC administration, particularly the role of lymphatic transport, may hinder the development of SC formulations for mAbs. The current study aimed to examine the absorption route of widely used anti-PD-(L)1 mAbs following SC injection, and four marketed products (Pembrolizumab, Tislelizumab, Envafolimab, Sugemalimab) were selected as the model drugs. In the thoracic lymph duct cannulated rat model, all mAbs were found to be primarily absorbed via the lymphatics following SC injection, with 63.1–72.0% of the administered dose recovered in the thoracic lymph, contributing to >80% of SC bioavailability. This absorption pattern also resulted in elevated exposure of mAbs in draining lymph nodes (an order of magnitude higher drug concentrations compared to the systemic drug exposure). In addition, markedly different lymphatic absorption profiles were observed following SC administration at different sites, reflecting specific draining routes associated with certain anatomical regions in the rat. Our data suggested that the food pad is likely the optimal SC injection site for quantitative studies of lymphatic transport, whereas injection to the lower hind leg (a common SC injection site in previous studies) appeared to underestimate the absolute extent of lymphatic drug transport in the thoracic lymph-duct cannulated rat model, as these sites are drained by lymphatic networks that cannot be fully captured by the thoracic lymph-duct cannulation model.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2024
·
IF 4.1
Jianguo Wu,Xin Li,Kun Fu,Dong Cao,Daojian Cheng
Pd-based catalysts have been widely applied in selective hydrogenation of phenol. However, avoiding excessive hydrogenation of C=O functional groups is still challenging. Here, we synthesized Pd nanoclusters supported by N-doped C-Al 2 O 3 (Pd/CN-Al 2 O 3 ) catalyst for phenol hydrogenation. Remarkably, the cyclohexanone yield shows a volcano trend with the doping of carbon content, where Pd/CN-Al 2 O 3 with carbon content of 6.76 % (Pd/CN-Al 2 O 3 -2) exhibits the highest cyclohexanone yield. Under the optimized conditions, the phenol conversion and cyclohexanone selectivity over Pd/CN-Al 2 O 3 -2 are 99.5 % and 96.7 %, respectively. The corresponding TOF value is 1731.7 h −1 , which is 5.8 times higher than that of Pd/Al 2 O 3 (299.7 h −1 ). Mechanism studies reveal the excellent catalytic performance of Pd/CN-Al 2 O 3 -2 is mainly attributed to the synergistic effect of electron-deficient Pd clusters and Lewis acid sites. Pd nanoclusters with moderate electron-deficient state and moderate Lewis acid density are beneficial to the adsorption of phenol and the desorption of cyclohexanone, thereby exhibiting the best phenol conversion and cyclohexanone selectivity. This work provides a carbon modification strategy to regulate the structure of Pd-based catalysts, which is a helpful guide for the hydrogenation reactions.
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Lanqing Li,Huijun Zheng,Lili Guo,Lingbo Qu,Lanlan Yu
A special electrochemical sensor was developed based on molecularly imprinted polymer (MIP), which was formed by the dopamine (DA) self-polymerization on the surface of Pd-Cu bimetallic alloy functionalized poly (diallyldimethylammonium chloride) -dispersed graphene (PDDA-Gr). This sensor takes advantages of molecularly imprinted technique and nanocomposite to realize the application of amaranth determination with high selectivity and sensitivity. In this study, the nanocomposite PDDA-Gr-(Pd-Cu) characterized by various techniques provides large surface area and accelerates the electron transfer process. The imprinted composite deposited on the surface of PDDA-Gr-(Pd-Cu), not only provides abundant specific cavities to amaranth , but also decreases the thickness of molecularly imprinted polymer leading to high conductivity. The electrochemical behavior of amaranth on the modified electrode was studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). After optimization, the anodic peak current showed a linear relationship with the concentration of amaranth in the range of 0.006–10 μM, and the limit of detection (LOD) was 2 nM. The proposed sensor distinguished amaranth from other structurally similar substances such as sunset yellow and tartrazine with good results. In addition, the molecularly imprinted sensor proposed in this study was successfully applied to determine amaranth in the soft drink with satisfactory recoveries.
✓ 已验证
DOI
高影响因子
Advanced Science
·
2025
·
IF 14.1
Tao Qin,Lian Qin,Yang Zhao,Yin Chen,Qianyue Liu,Xiaoguang Lin,Yongfeng Lan,Yaohui Huang,Yan Liu,Ke Zhang,Lifan Pan,Jiaxiao Li,Kunpeng Duan,Hao Liang,Mingjing Yin,Guiyang Fan,Lian Liu,Yu Deng,Lin Liao,Danke Su,Ming Gao,Junyu Lu
Sepsis induced acute liver injury (SALI), is a type of acute and severe disease that is generally characterized by producing significant amounts of reactive oxygen species (ROS) in liver tissue, and in response to excessive ROS, producing huge amounts of inflammatory factors by hepatocytes. Considering the crucial role of ROS in SALI, a Pd doped CeO 2 (CP) nano-heterojunction with enhanced ROS scavenging capacity is developed to act as a catalytic nanomedicine for the treatment of SALI. Combining with near infrared (NIR) irradiation, it exhibits excellent scavenging capacity of ROS including hydroxyl radical (•OH), superoxide anion (•O 2 − ), as well as singlet oxygen ( 1 O 2 ) for CP. Significantly, it also demonstrates the excellent antioxidant and anti-inflammatory activities for lipopolysaccharides (LPS) stimulated macrophages (RAW264.7), and cecum ligation and puncture (CLP) treated C57BL/6J mice via reducing intracellular ROS levels, decreasing inflammatory factors expression levels, as well as activating Keap1/Nrf-2/HO-1 pathway to reprogram redox homeostasis, induce cellular autophagy, reduce systemic inflammation and promote liver tissue repair, finally achieving the alleviation of SALI. It provides a promising therapeutic strategy of CP+NIR with high efficacy and biosafety for the management of SALI.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2026
·
IF 6.1
Chenxi Xie,Dong Zhang,Yijiang Wu,Yuan He,Yiman Ge,Bin Deng
In this study, a highly sensitive surface-enhanced Raman scattering (SERS) microarray chip was developed for the detection of reduced glutathione (GSH) in the serum of colorectal cancer (CRC) patients. Gold-palladium nanorods (Au@Pd NRs) with peroxidase-like activity were synthesized and assembled into holes on the polydimethylsiloxane (PDMS)-indium tin oxide (ITO) glass substrate via electrophoretic deposition (EPD). The Au@Pd NRs catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to oxidized TMB (oxTMB) in the presence of hydrogen peroxide (H 2 O 2 ), producing a characteristic SERS peak at 1605 cm −1 . Prior to detection, a thiolated GSH aptamer was immobilized on the microarray chip to specifically capture GSH, effectively avoiding interference from other reducing substances in the complex serum matrix. The captured GSH then reduces oxTMB back to TMB, causing signal attenuation proportional to GSH concentration. The limit of detection (LOD) reached 93 pmol L −1 , with a relative standard deviation (RSD) of 6.36% for reproducibility. Clinical serum analysis of 30 healthy individuals and 30 CRC patients demonstrated results consistent with the gold standard testing method of high-performance liquid chromatography (HPLC) (relative error < 8%), confirming the potential of this approach for the effective detection of GSH and early CRC diagnosis. In this study, a highly sensitive surface-enhanced Raman scattering (SERS) microarray chip was developed for the detection of reduced glutathione (GSH) in the serum of colorectal cancer (CRC) patients.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Molecular Catalysis
·
2023
·
IF 4.6
Jia Li,Jianfeng Zheng,Xingmin Liu,Zhanggen Huang
Metal additives can greatly improve the catalytic oxidation of photocatalysts for degrading volatile organic compounds (VOCs) including formaldehyde. Different type metal, including Pd, Cu and Na, were doped in TiO 2 (B) nanosheets with oxygen vacancy, and the degradation rate of HCHO were evaluated using the above catalysts. A series of theoretical and experimental studies on these modified TiO 2 (B) photocatalysts were carried out. The presence of O v leads to the appearance of impurity levels under the conduction band of TiO 2 (B), which could enhance the absorption in the visible spectral range by reducing the band gap and inhibiting the recombination of photo-induced charges. TiO 2 (B) with oxygen vacancies and doped with Na is used as a photocatalyst for degrading HCHO, and the removal rate up to 80% within 50 min. It is quite important to match the bandgap structure of photocatalysts and redox potential of O 2 /•O 2 − . Doping of excessive metals is detrimental to the separation and transfer of photogenerated charges. The intermediates and mechanism of photocatalytic oxidation of HCHO were investigated by in situ DRIFTS.
✓ 已验证
产品关联
DOI
高影响因子
Nano Today
·
2022
·
IF 18.962
Di Liu,Hongliang He,Fei Kong,Yixiang Cao,Fengchao Zang,Ming Ma,Ning Gu,Yu Zhang
Currently, multimodal synergistic nanoplatform emerges as an important cancer therapy paradigm, however, multimodal synergistic theranostic nanoplatform remains to be developed. Herein, using the mouse model of breast cancer, a versatile multimodal synergistic theranostic nanoplatform is rationally designed and synthesized for enhancing chemodynamic therapy (CDT), overcoming immunosuppression within tumor microenvironment , and inhibiting metastasis as well as tracking tumor. To fulfill our design, a composite Fe/Mn magnetic nanoparticle is first synthesized by loading with Fe 3 O 4 and BMS-202 (PD-L1 inhibitor) within a poly(lactide-co-glycolide)-based nanoparticle core, which are further modified with in situ synthesis of MnO 2 layer. Then, the composite metal-organic nanoparticle is coated with two targeting moieties hyaluronic acid (HA) and AMD3100 (CXCR4 antagonist), respectively, to achieve the multimodal synergistic theranostic nanoplatform ( [email protected] +AMD). With surface targeting modification, [email protected] +AMD exhibits enhanced tumor accumulation, where it effectively consumes endogenous glutathione to generate Mn 2+ allowing for the enhanced CDT effect, alleviates tumor hypoxia by O 2 generation and reverses the tumor immunosuppression. [email protected] +AMD blocks CXCR4 receptor on cancer cells, thus suppressing the CXCR4-mediated cancer metastasis and invasion. Additionally, [email protected] +AMD would synchronously be achieving tumor tracking by T 1 -T 2 dual-mode magnetic resonance imaging. Collectively, this strategy holds a novel multimodal synergistic theranostics for effective cancer management.
✓ 已验证
产品关联
DOI
高影响因子
Antibiotics-Basel
·
2022
·Vol. 11
·期 11
·1660
·
IF 5.222
Yan Wang,Lanyu Liu,Qiping Wu,Qiufen Yin,Feifan Xie
Background: The pharmacokinetic/pharmacodynamic (PK/PD) index of carbapenems that best correlates with in vivo antimicrobial activity is percent time of dosing interval in which free drug concentration remains above MIC (%fT > MIC), while the magnitudes of the PK/PD index of carbapenems remains undefined in critically ill sepsis patients.Methods: A sepsis rat model was first developed by comparing the survival outcomes after intraperitoneal injection of different inoculum size (1–10 × 107CFU) ofPseudomonas aeruginosaATCC9027 (MIC = 0.125 mg/L) in neutropenic rats. The PK characteristics of the model drug meropenem in the developed sepsis rat model was then evaluated, and PK modeling and simulation was applied to design meropenem dosing regimens attaining various PD targets (40%fT > MIC, 100%fT > MIC, and 100%fT > 4 × MIC). The microbiological response and survival outcomes for different meropenem treatment regimens were investigated in the rat sepsis model (n= 12 for each group).Results: The optimal inoculum for the rat sepsis model was 1 × 107CFU ofPseudomonas aeruginosaATCC9027. A one-compartment model with first-order absorption best described the PK of meropenem in sepsis rats. Pronounced survival prolongation and lower hazard risk were observed in the treatment groups of 50 or 75 mg/kg/q2.4h (100%fT > MIC) and 75 mg/kg/q2h (100%fT > 4 × MIC) compared to the 75 mg/kg/q6h (40%fT > MIC) group, while meropenem groups with PD targets of 100%fT > MIC and 100%fT > 4 × MIC showed comparable survival curves. Microbiological response for different PD targets is inconclusive due to irregular bacterial counts in blood samples.Conclusions: The PD target of 40%fT > MIC is suboptimal for sepsis rats, and the aggressive 100%fT > 4 × MIC target does not provide a survival benefit against the target of 100%fT > MIC.Keywords:pharmacokinetics;PK/PD;survival;meropenem;sepsis rat model
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Asian Journal of Pharmaceutical Sciences
·
2022
·
IF 9.273
Xudong Fan,Tianxiang Yue,Aidi Liu,Xiaowei Xie,Weixiang Fang,Yinghui Wei,Hangsheng Zheng,Hongyue Zheng,Meiqi Zhou,Jigang Piao,Fanzhu Li
Photothermal therapy (PTT) has brought hope for cancer treatments, with hyperthermia-induced immunogenic cell death (ICD), which is a critical part of therapeutically induced antitumor immune responses. Limited immune stimulation response in PTT is the primary reason for incomplete tumor ablation, therefore demonstrating urgent requirements for ICD amplifier. Herein, a sub-10 nm supramolecular nanoassembly was formed by co-assembly of clinically approved aluminum adjuvant and commonly used indocyanine green (ICG) under the assistance of lignosulfonate (LS, a green and sustainable multifunctional lignin derivative) for localized photothermal-immunotherapy of breast cancer. The overall results revealed that LS-Al-ICG is capable of inducing amplified ICD, efficiently eliciting solid immune responses through dendritic cells (DCs) activation and cytotoxic T-cell responses initiation for tumor killing. Moreover, anti-PD-1 therapy blocked the PD-1 pathway and led to remarkable anti-tumor efficacy against laser-irradiated primary tumors and distant tumors by potentiating systemic tumor specific T cell immunity. The results of this study demonstrate a handy and extensible approach for engineering green natural lignin nanoparticles for cancer immunotherapy, which shows promise for delivering other therapeutics in biomedical applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 16
·7695-7703
·
IF 3.925
Qi Wu,Changfeng Zhao,Qinzhong Wang,Jing Liu
This study proposes the efficient separation of Au(III)/Pd(II)/Pt(IV)/Rh(III) through the 2-mercaptobenzothiazole-functionalised ionic liquid ([C6mim][2MBT]) using a cloud point extraction system. Direct cloud point extraction of Au(III) (about 99.9%) was realised without involving any functionalised ionic liquids. A significant enhancement of the Pd(II) extraction performance was found by adding rationally constructed [C6mim][2MBT]. Consequently, a fairly high extraction percentage of about 99.4% was obtained. However, further advancement of the proposed cloud point extraction showed that it could not be applied for the preconcentration of Pt(IV) and Rh(III) without adding stannous chloride, which plays a critical role in the segregation and could be adjusted by changing the hydrochloric acid concentration. Under the optimised conditions, the Rh(III) extraction process shows a high yield percentage (ca. 83.0%), where the majority of Pt(IV) was enriched in the aqueous phase. In addition, the underlying possible extraction mechanism was investigated using UV-vis, FT-IR and TEM measurements, which indicated that TX-114 acted as a solvent for the extracted metals. The developed procedure reveals efficient direct Au(III)/Pd(II)/Pt(IV)/Rh(III) separation using [C6mim][2MBT] via cloud point extraction.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL PAPERS
·
2023
·Vol. 77
·期 11
·6637-6646
·
IF 2.2
Chen Lei,Li Rongxin,Li Yuanlong,Shu Qijiang,Yang Tao
The reduced graphene oxide aerogel modified by 4-chlorophenyl sulfoxide was obtained by a simple self-assemble process under the basic reduction-oxide condition, then freeze-dried for future using. The prepared aerogel was shortly named 4CS-rGO, which was characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, scanning electron microscope, et al. 1 mg of 4CS-rGO composite was used as an adsorbent to adsorb Pd(II) and Pt(IV) in 1.0 mL 40 mM Britton-Robinson buffer solution with various pH. The adsorption efficiency of Pd(II) increased with the increase in pH, whereas the hydrolysis of Pd(II) happened at pH 13, and the maximum adsorption efficiency of Pd(II) was 91.82% at pH 12, which was mainly driven by electrostatic attraction. Besides, the adsorption behavior of the 4CS-rGO composite for Pt(IV) was explored to evaluate the possibility to isolate Pd(II) and Pt(IV) ions by using aerogel. We found that, quite interestingly, hydrogen bond, hydrophobic interaction and van der Waals force can strongly conquer electrostatic repulsion driven Pt(IV) ions adsorption behavior at pH 5, and Pt(IV) ions displaced by cationic surfactant. Moreover, a tighter Pt(IV) ions adsorption behavior than Pd(II) ions was observed while we adsorb them respectively with/without 4-chlorophenyl sulfoxide modified the graphene, while branched TBAB was easier and more effectively replaced the adsorbed Pt(IV) ions than strained CTAB.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2021
·
IF 3.935
Xinying He,Han Cao,Xia Li,Yan Li,Yunqiu Yu
Lysophosphatidylcholine (LPC) was commonly known as a class of significant differential metabolites of high relevance with many diseases including psoriasis, of which the accurate determination is of great importance to diagnosis or prediction to many diseases. However, it is challenging and complicated because of the enormous biological sample complexity and impurities interference. In this study, we synthesized a magnetic nanocomposite MG@PD@TiO 2 and took advantage of the interactions of Lewis acid−base between the phosphate groups in LPCs and Ti ions on MG@PD@TiO 2 nanomaterials for selective separation and enrichment of LPCs from complex biological matrix. The solid-phase extraction sample pretreatment process by means of MG@PD@TiO 2 nanomaterials coupled with LC–MS/MS method was then applied to actual determination of six typical LPCs (LPC 10:0, 14:0, 16:0, 18:0, 18:1, 22:0) in human plasma. The extraction conditions were scientifically optimized by single-factor test (adsorbent amount, adsorption and desorption time, elution solvent type, eluant volume). Under the optimal conditions, the detection limits (LOD, S/N = 3) and quantification limits (LOQ, S/N = 10) were 1 and 5 ng/mL for LPC 10:0 and LPC 14:0, 0.02 and 0.1 ng/mL for LPC 16:0 and LPC 18:1, 0.05 and 0.2 ng/mL LPC 18:0 and LPC 22:0, respectively. The intra- and inter-day precisions were 3.82–12.60 % (n = 6) and 3.29–13.50 % (n = 6) respectively, the recoveries were in the range of 91.92–113.69 % and the stability of the analytes in the matrix performed well with RSDs≤15.51 %. Finally, the developed method was successfully applied to the accurate determination of six LPCs biomarkers of plasma in patients with psoriasis (n = 10) and control groups (n = 10).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）