SENSORS AND ACTUATORS B-CHEMICAL
·
2016
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IF 4.758
Li Liu,Yueyuan Li,Lihui Tian,Qin Wei,Wei Cao
A novel and ultrasensitive sandwich-type electrochemical immunosensor was designed for quantitative detection of prostate specific antigen (PSA). The Ag overgrowth in Pd nano-octahedrons, developed Pd-Ag heterodimers (Pd-Ag HDs), loaded on amino functionalized multiwalled carbon nanotubes (MWCNTs-NH 2 ) were employed as labels of secondary antibodies (Ab 2 ). The synergistic effect of Pd-Ag HDs exhibited high electrocatalytic activity towards the reduction of H 2 O 2 . MWCNTs-NH 2 with good dispersion , plentiful amino groups and large specific surface area , could load a large number of Pd-Ag HDs to enhance the sensitivity and amplify the signal of the immunosensor. Moreover, the electroplated gold nanoparticles (Au NPs) were employed for the immobilization of primary antibodies (Ab 1 ). Au NPs with excellent conductivity could enhance the electron transport between substrate material and electrode surface , and improve the sensitivity of immunosensor. Under optimal conditions, the electrochemical immunosensor exhibited a wide linear range of 0.1 pg/mL–30 ng/mL towards PSA, and a low detection limit of 0.03 pg/mL (S/N = 3). The proposed immunosensor exhibited good sensitivity, reproducibility and stability for the quantitative detection of PSA, which hold a great potential in clinical and diagnostic applications.
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JOURNAL OF PHARMACEUTICAL SCIENCES
·
2025
·
IF 3.8
Xiaoxuan Fei,Junyi Pei,Simin Yan,Weihong Ge,Li Li,Jianjun Zou,Huimin Zhang,Lie Sun,Sifei Han,Luojuan Hu
Compared to classic intravenous (IV) administration, subcutaneous (SC) delivery of monoclonal antibodies (mAbs) offers new opportunities to improve patient compliance and healthcare efficiency. However, insufficient understanding of drug absorption process following SC administration, particularly the role of lymphatic transport, may hinder the development of SC formulations for mAbs. The current study aimed to examine the absorption route of widely used anti-PD-(L)1 mAbs following SC injection, and four marketed products (Pembrolizumab, Tislelizumab, Envafolimab, Sugemalimab) were selected as the model drugs. In the thoracic lymph duct cannulated rat model, all mAbs were found to be primarily absorbed via the lymphatics following SC injection, with 63.1–72.0% of the administered dose recovered in the thoracic lymph, contributing to >80% of SC bioavailability. This absorption pattern also resulted in elevated exposure of mAbs in draining lymph nodes (an order of magnitude higher drug concentrations compared to the systemic drug exposure). In addition, markedly different lymphatic absorption profiles were observed following SC administration at different sites, reflecting specific draining routes associated with certain anatomical regions in the rat. Our data suggested that the food pad is likely the optimal SC injection site for quantitative studies of lymphatic transport, whereas injection to the lower hind leg (a common SC injection site in previous studies) appeared to underestimate the absolute extent of lymphatic drug transport in the thoracic lymph-duct cannulated rat model, as these sites are drained by lymphatic networks that cannot be fully captured by the thoracic lymph-duct cannulation model.
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TALANTA
·
2019
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IF 4.916
Lanqing Li,Huijun Zheng,Lili Guo,Lingbo Qu,Lanlan Yu
A special electrochemical sensor was developed based on molecularly imprinted polymer (MIP), which was formed by the dopamine (DA) self-polymerization on the surface of Pd-Cu bimetallic alloy functionalized poly (diallyldimethylammonium chloride) -dispersed graphene (PDDA-Gr). This sensor takes advantages of molecularly imprinted technique and nanocomposite to realize the application of amaranth determination with high selectivity and sensitivity. In this study, the nanocomposite PDDA-Gr-(Pd-Cu) characterized by various techniques provides large surface area and accelerates the electron transfer process. The imprinted composite deposited on the surface of PDDA-Gr-(Pd-Cu), not only provides abundant specific cavities to amaranth , but also decreases the thickness of molecularly imprinted polymer leading to high conductivity. The electrochemical behavior of amaranth on the modified electrode was studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). After optimization, the anodic peak current showed a linear relationship with the concentration of amaranth in the range of 0.006–10 μM, and the limit of detection (LOD) was 2 nM. The proposed sensor distinguished amaranth from other structurally similar substances such as sunset yellow and tartrazine with good results. In addition, the molecularly imprinted sensor proposed in this study was successfully applied to determine amaranth in the soft drink with satisfactory recoveries.
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RSC Advances
·
2026
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IF 6.1
Chenxi Xie,Dong Zhang,Yijiang Wu,Yuan He,Yiman Ge,Bin Deng
In this study, a highly sensitive surface-enhanced Raman scattering (SERS) microarray chip was developed for the detection of reduced glutathione (GSH) in the serum of colorectal cancer (CRC) patients. Gold-palladium nanorods (Au@Pd NRs) with peroxidase-like activity were synthesized and assembled into holes on the polydimethylsiloxane (PDMS)-indium tin oxide (ITO) glass substrate via electrophoretic deposition (EPD). The Au@Pd NRs catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to oxidized TMB (oxTMB) in the presence of hydrogen peroxide (H 2 O 2 ), producing a characteristic SERS peak at 1605 cm −1 . Prior to detection, a thiolated GSH aptamer was immobilized on the microarray chip to specifically capture GSH, effectively avoiding interference from other reducing substances in the complex serum matrix. The captured GSH then reduces oxTMB back to TMB, causing signal attenuation proportional to GSH concentration. The limit of detection (LOD) reached 93 pmol L −1 , with a relative standard deviation (RSD) of 6.36% for reproducibility. Clinical serum analysis of 30 healthy individuals and 30 CRC patients demonstrated results consistent with the gold standard testing method of high-performance liquid chromatography (HPLC) (relative error < 8%), confirming the potential of this approach for the effective detection of GSH and early CRC diagnosis. In this study, a highly sensitive surface-enhanced Raman scattering (SERS) microarray chip was developed for the detection of reduced glutathione (GSH) in the serum of colorectal cancer (CRC) patients.
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Advanced Science
·
2025
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IF 14.1
Tao Qin,Lian Qin,Yang Zhao,Yin Chen,Qianyue Liu,Xiaoguang Lin,Yongfeng Lan,Yaohui Huang,Yan Liu,Ke Zhang,Lifan Pan,Jiaxiao Li,Kunpeng Duan,Hao Liang,Mingjing Yin,Guiyang Fan,Lian Liu,Yu Deng,Lin Liao,Danke Su,Ming Gao,Junyu Lu
Sepsis induced acute liver injury (SALI), is a type of acute and severe disease that is generally characterized by producing significant amounts of reactive oxygen species (ROS) in liver tissue, and in response to excessive ROS, producing huge amounts of inflammatory factors by hepatocytes. Considering the crucial role of ROS in SALI, a Pd doped CeO 2 (CP) nano-heterojunction with enhanced ROS scavenging capacity is developed to act as a catalytic nanomedicine for the treatment of SALI. Combining with near infrared (NIR) irradiation, it exhibits excellent scavenging capacity of ROS including hydroxyl radical (•OH), superoxide anion (•O 2 − ), as well as singlet oxygen ( 1 O 2 ) for CP. Significantly, it also demonstrates the excellent antioxidant and anti-inflammatory activities for lipopolysaccharides (LPS) stimulated macrophages (RAW264.7), and cecum ligation and puncture (CLP) treated C57BL/6J mice via reducing intracellular ROS levels, decreasing inflammatory factors expression levels, as well as activating Keap1/Nrf-2/HO-1 pathway to reprogram redox homeostasis, induce cellular autophagy, reduce systemic inflammation and promote liver tissue repair, finally achieving the alleviation of SALI. It provides a promising therapeutic strategy of CP+NIR with high efficacy and biosafety for the management of SALI.
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NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 16
·7695-7703
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IF 3.925
Qi Wu,Changfeng Zhao,Qinzhong Wang,Jing Liu
This study proposes the efficient separation of Au(III)/Pd(II)/Pt(IV)/Rh(III) through the 2-mercaptobenzothiazole-functionalised ionic liquid ([C6mim][2MBT]) using a cloud point extraction system. Direct cloud point extraction of Au(III) (about 99.9%) was realised without involving any functionalised ionic liquids. A significant enhancement of the Pd(II) extraction performance was found by adding rationally constructed [C6mim][2MBT]. Consequently, a fairly high extraction percentage of about 99.4% was obtained. However, further advancement of the proposed cloud point extraction showed that it could not be applied for the preconcentration of Pt(IV) and Rh(III) without adding stannous chloride, which plays a critical role in the segregation and could be adjusted by changing the hydrochloric acid concentration. Under the optimised conditions, the Rh(III) extraction process shows a high yield percentage (ca. 83.0%), where the majority of Pt(IV) was enriched in the aqueous phase. In addition, the underlying possible extraction mechanism was investigated using UV-vis, FT-IR and TEM measurements, which indicated that TX-114 acted as a solvent for the extracted metals. The developed procedure reveals efficient direct Au(III)/Pd(II)/Pt(IV)/Rh(III) separation using [C6mim][2MBT] via cloud point extraction.
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Asian Journal of Pharmaceutical Sciences
·
2022
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IF 9.273
Xudong Fan,Tianxiang Yue,Aidi Liu,Xiaowei Xie,Weixiang Fang,Yinghui Wei,Hangsheng Zheng,Hongyue Zheng,Meiqi Zhou,Jigang Piao,Fanzhu Li
Photothermal therapy (PTT) has brought hope for cancer treatments, with hyperthermia-induced immunogenic cell death (ICD), which is a critical part of therapeutically induced antitumor immune responses. Limited immune stimulation response in PTT is the primary reason for incomplete tumor ablation, therefore demonstrating urgent requirements for ICD amplifier. Herein, a sub-10 nm supramolecular nanoassembly was formed by co-assembly of clinically approved aluminum adjuvant and commonly used indocyanine green (ICG) under the assistance of lignosulfonate (LS, a green and sustainable multifunctional lignin derivative) for localized photothermal-immunotherapy of breast cancer. The overall results revealed that LS-Al-ICG is capable of inducing amplified ICD, efficiently eliciting solid immune responses through dendritic cells (DCs) activation and cytotoxic T-cell responses initiation for tumor killing. Moreover, anti-PD-1 therapy blocked the PD-1 pathway and led to remarkable anti-tumor efficacy against laser-irradiated primary tumors and distant tumors by potentiating systemic tumor specific T cell immunity. The results of this study demonstrate a handy and extensible approach for engineering green natural lignin nanoparticles for cancer immunotherapy, which shows promise for delivering other therapeutics in biomedical applications.
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EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2021
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IF 4.384
Min Zhang,Zhou Fang,Haitao Zhang,Mingxiao Cui,Mingfu Wang,Kehai Liu
In order to reverse tumor immunosuppressive microenvironment and improve antitumor immune effect based on immune checkpoint blocking, a mannose-modified liposome-based CpG ODNs and PD-L1 antagonistic peptides (P) co-delivery system (HA/M-Lipo CpG-P) was constructed, in which hyaluronic acid (HA) coating was supposed to improve the systemic circulation stability and thereby promote its accumulation in tumor tissues. When the HA/M-Lipo CpG-P complexes enter the tumor tissues, HA will be hydrolyzed under the action of hyaluronidase, exposing P peptides. Then, P peptides linked by octapeptides that can be cleaved by matrix metalloproteinases (MMPs) are released into tumor tissues under the action of MMPs, exerting a blocking effect in the PD-1/PD-L1 pathway. The M-Lipo CpG complexes can recognize macrophage surface mannose receptors through its surface modified mannose molecules, and promote the intracellular delivery of CpG ODNs, thereby activating macrophages. The results showed that HA/M-Lipo CpG-P complexes successfully reversed M2-type macrophages in tumor microenvironment (TME) to M1, thereby activating anti-tumor related immune cells and inhibiting tumor growth. Moreover, the HA/M-Lipo CpG-P complexes showed a better tumor inhibitory effect than the HA/M-Lipo CpG or the HA/M-Lipo-P (monotherapy) treatment groups. Overall, HA/M-Lipo CpG-P complexes provide a promising co-delivery strategy for targeting tumors to improve the antitumor effect based on immune checkpoint blockade.
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Journal of Environmental Chemical Engineering
·
2021
·
IF 5.909
Lin Hu,Xian-Yong Wei,Mei-Ling Xu,Yu-Hong Kang,Xian-Hou Guo,Feng-Bin Zhang,Zhi-Min Zong,Hong-Cun Bai
The selective hydrodeoxygenation (HDO) of lignin-derived bio-oil still meets the great challenges due to its complex oxygenated organic component. In this research, first time, Pd-based zeolite catalysts were prepared by the modified deposition-precipitation (DP) method for the HDO conversion of guaiacol. Notably, for the same Si/Al ratio of zeolite, Pd 2% /Hβ catalyst showed the higher catalytic activity on the HDO of guaiacol than that Pd 2% /M and Pd 2% /HZSM-5 catalyst, which mainly originated from the synergetic effect between metal nanoparticles size and acid sites of catalyst. As one of typical phenolic monomers , guaiacol can be converted into high yield of hydrocarbons under the condition of 220 °C, 3 MPa H 2 , and 4 h in n -hexane over Pd 2% /Hβ (DP) catalyst. This highly active catalyst exhibited the good recyclability in the HDO conversion of guaiacol. In addition, other typical phenolic monomers can be converted into high yield of hydrocarbons over this highly active catalyst. Considering this, after HDO upgrading, real lignin oil (RLO) can be converted into high yield of hydrocarbons with the carbon number (CN) in range of C7-C15. This work highlighted that the depolymerization monomers from lignin can be further upgraded by this high active catalyst through HDO to obtain jet hydrocarbons fuel .
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Frontiers in Oncology
·
2021
·
IF 6.244
Yang Jianru,Jia Yang,Wang Bing,Yang Shengrong,Du Kun,Luo Yujie,Li Yunhe,Zhu Bing
Background: The effects of up-regulated CircCHST15 on lung cancer remained unclear. In this study, the role of CircCHST15 in lung cancer was investigated.Methods: Dual-luciferase reporter verified the bioinformatics prediction that CircCHST15 targeted miR-155-5p and miR-194-5p. The correlation between CircCHST15 and PD-L1 was analyzed by Pearson analysis. CCK-8 and colony formation was performed to determine the viability and proliferation of lung cancer cells. After the lung cancer (subcutaneous-xenotransplant) model was established in mice, the T cell subtype and related cytokines in mouse tumor tissues were detected by flow cytometry and ELISA. Moreover, the expressions of CircCHST15, miR-155-5p, miR-194-5p, immune-related, and proliferation-related factors of the lung cancer cells or mice tumor tissues were detected by immunohistochemistry, RT-qPCR, or Western blot.Results: CircCHST15 and PD-L1 were high-expressed in lung cancer, and the two was positively correlated. CircCHST15 targeted miR-155-5p and miR-194-5p, the later further targeted PD-L1. Lung cancer cell viability and proliferation were increased by miR-155-5p and inhibited by miR-194-5p. CircCHST15 located in the cytoplasm promoted tumor growth, down-regulated the expressions of miR-155-5p and miR-194-5p, and up-regulated the expressions of PD-L1, Ki-67, PCNA, CCL17, CCL22, IFN-γ, TNF-β, and IL-10. Also, CircCHST15 decreased the CD8+ cells in mouse blood and tumor, but increased the Tregs in mouse tumor. PD-L1 inhibitor showed an opposite effect to CircCHST15 on mouse tumors.Conclusion: CircCHST15 sponged miR-155-5p and miR-194-5p to promote the PD-L1-mediated immune escape of lung cancer cells.
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APPLIED SURFACE SCIENCE
·
2021
·
IF 6.707
Xiqiang Tian,Muhammad Zahid,Wang Sun,Yujun Zhu
TiO 2 with different morphology as supports can lead to different surface properties and catalytic activities of catalysts. Herein, we reported a novel facile evaporation-induced assisted hydrothermal approach to synthesize three-dimensional hierarchical structure TiO 2 assembled from needle flakes by using Brij-35 as the morphology-directing agent. The morphology of the prepared TiO 2 from flower-like (TiO 2 -FT), sea urchin-like (TiO 2 -SUT), to ball-like (TiO 2 -BT), as well as the size of needle flakes, were controlled by adjusting the amount of Brij-35. These TiO 2 supported palladium catalysts were facilely prepared using a green bio-reductive method with Catalpa Fruit extract as reducing agent, where they showed remarkable catalytic activity in selective hydrogenation of 4-nitrophenol by sodium borohydride. Especially, 1 wt%Pd/TiO 2 -SUT with sea urchin-like exhibited higher reaction rate constant (1.44 min −1 ) than 1 wt%Pd/TiO 2 -FT (1.08 min −1 ) and 1 wt%Pd/TiO 2 -BT (0.72 min −1 ). Furthermore, XRD, N 2 adsorption–desorption, SEM, TEM, CO-FTIR, XPS and CO chemisorption measurements were performed to study the effects of different surface morphologies on the surface properties of catalysts. It reveals that the high catalytic performance of 1 wt%Pd/TiO 2 -SUT can be attributed to its specific hierarchical structure and much higher surface area, improving the uniform dispersion of Pd nanoparticles on the support to expose extra active surface Pd atoms (Pd 0 species) over 1 wt%Pd/TiO 2 -SUT.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2021
·
IF 3.935
Xinying He,Han Cao,Xia Li,Yan Li,Yunqiu Yu
Lysophosphatidylcholine (LPC) was commonly known as a class of significant differential metabolites of high relevance with many diseases including psoriasis, of which the accurate determination is of great importance to diagnosis or prediction to many diseases. However, it is challenging and complicated because of the enormous biological sample complexity and impurities interference. In this study, we synthesized a magnetic nanocomposite MG@PD@TiO 2 and took advantage of the interactions of Lewis acid−base between the phosphate groups in LPCs and Ti ions on MG@PD@TiO 2 nanomaterials for selective separation and enrichment of LPCs from complex biological matrix. The solid-phase extraction sample pretreatment process by means of MG@PD@TiO 2 nanomaterials coupled with LC–MS/MS method was then applied to actual determination of six typical LPCs (LPC 10:0, 14:0, 16:0, 18:0, 18:1, 22:0) in human plasma. The extraction conditions were scientifically optimized by single-factor test (adsorbent amount, adsorption and desorption time, elution solvent type, eluant volume). Under the optimal conditions, the detection limits (LOD, S/N = 3) and quantification limits (LOQ, S/N = 10) were 1 and 5 ng/mL for LPC 10:0 and LPC 14:0, 0.02 and 0.1 ng/mL for LPC 16:0 and LPC 18:1, 0.05 and 0.2 ng/mL LPC 18:0 and LPC 22:0, respectively. The intra- and inter-day precisions were 3.82–12.60 % (n = 6) and 3.29–13.50 % (n = 6) respectively, the recoveries were in the range of 91.92–113.69 % and the stability of the analytes in the matrix performed well with RSDs≤15.51 %. Finally, the developed method was successfully applied to the accurate determination of six LPCs biomarkers of plasma in patients with psoriasis (n = 10) and control groups (n = 10).
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