JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Mengyao Qi,Baihui Wang,Huan Jiang,Yifei Li,Peng Li,Xia Zhang,Lei Han
Ascorbic acid (AA) is an essential micronutrient , which is widely used as an additive in beverages and medicines to improve food quality and human health. Herein, we developed a smartphone readable colorimetry and Surface-enhanced Raman scattering (SERS) dual-mode platform based on GeO 2 @Fe 3 O 4 /Au NPs nanocomposite to detect AA. GeO 2 @Fe 3 O 4 /Au NPs have excellent peroxidase-like activity and can oxidize colorless 3,3′,5,5′-tetramethylbenzidine into blue 3,3′,5,5′-tetramethylbenzidine oxide. AA can inhibit the oxidation of 3,3′,5,5′-tetramethylbenzidine, which lead to fading. Based on the above principles, we developed an innovation for micro-detection of AA using oxidized 3,3′,5,5′-tetramethylbenzidine as probe molecule, combined with smartphone readable colorimetry and SERS dual-mode detection. Under optimum conditions, the detection range of AA is 0.5 μmol/L − 340 μmol/L and 10 −9 μmol/L − 10 0 mol/L, the detection limit is 0.6689 μmol/L and 6.162 × 10 −13 mol/L, respectively. The method was successfully used for AA in fruits, vitamin C beverages and vitamin C tablets, and the results are consistent with the prescribed values.
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Xinyu Fang,Jiali Ma,Chenjie Gu,Wei Xiong,Tao Jiang
Surface-enhanced Raman scattering (SERS) is a prospective and ultrasensitive analytical technique because of its prominent molecular fingerprint diagnosis ability. Nonetheless, the tested molecules are typically required to be steadily attached onto a SERS-active region, which makes it tough to monitor feebly adsorbed molecules . In this study, a polydimethylsiloxane (PDMS)-based flexible SERS substrate combined with silver nanocubes (Ag NCs) and ZIF-67 was developed with a facile layer-by-layer strategy. A superhigh enhancement factor (EF) of 9.1 × 10 8 M was obtained by electromagnetic enhancement from the promising Ag NCs in conjunction with the molecular anchoring surface and chemical enhancement ability of ZIF-67. Furthermore, the practical SERS-based detection of quercetin with an ultra-low limit of detection (LOD) of 2.3 × 10 −5 mg/mL was subsequently achieved utilizing this multifunctional substrate, indicating its intriguing outlook in the direct monitoring of food nutrients. Notably, the intrinsic Raman signal at 2905 cm −1 of PDMS framework was adequately utilized as an internal standard to promote the reliability of such detection. It is anticipated that this smart platform with both prominent SERS performance and quantitative test ability may find an extensive application in the domains of food and biochemical assay.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL SCIENCES
·
2023
·
IF 1.6
Xu Yang,Liu Yue,Luo Yu,Xu Xinlin,Li Yingying,Zhao Lin,Li Tiantian,Zhang Yan,He Peng,Mou Xiaoming
Lysozyme (LYS) is a widely used bacteriostatic enzyme. In this paper, we built a sensitive and accurate Raman biosensing platform to detect trace amounts of LYS. The method is based on magnetic spherical nucleic acid formed by a combination of LYS aptamer (Apt) and magnetic beads (MBs). Meanwhile, this method utilizes a dual enzyme-assisted nucleic acid amplification circuit and surface-enhanced Raman scattering (SERS). In this sensing strategy, which is based on the specific recognition of Apt, magnetic spherical nucleic acids were associated with SERS through a nucleic acid amplification circuit, and the low abundance of LYS was converted into a high-specificity Raman signal. Satellite-like MB@AuNPs were formed in the presence of the target, which separated specifically in a magnetic field, effectively avoided the interference of complex sample environment. Under the optimal sensing conditions, the concentration of LYS exhibited a good linear relationship between 1.0 × 10 –14 and 5.0 × 10 –12 M and the limit of detection was as low as 8.3 × 10 –15 M. In addition, the sensor strategy shows excellent accuracy and sensitivity in complex samples, providing a new strategy for the specific detection of LYS. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 35
·4524-4532
·
IF 3.1
Huipeng Deng,Zakir H. Talpur,Kaijun Wang,Yan Kang,Yiping Du,Dongmei Xu,Xinglong Fan,Wei Li
A novel magnetic agitating heater powered by a USB port has been developed and applied to synthesize silver colloid substrate for surface-enhanced Raman scattering (SERS) detection of sodium saccharin content on the tipping paper of cigarettes. The heater device allows the convenient synthesis of the Ag colloid, and the reaction can be completed on-site in 15 min under mild conditions. The on-site synthesis of SERS substrate effectively avoided the need for storage and concerns regarding the poor stability and short lifespan of colloid substrates. The results demonstrated that the substrate obtained with the device could achieve SERS detection of Rhodamine 6G (R6G) at as low as 10−9 mol L−1 while maintaining a stable intensity with a relative standard deviation (RSD) of 5.52% (n = 5). Using the prepared substrate at the optimal conditions, the limit of detection of sodium saccharin (SS) was 1 mg L−1. By introducing an internal standard KSCN, a linear relationship was observed between the relative intensity at 708 cm−1 and the concentration of the SS in a range of 20–100 mg L−1 (R2 = 0.98). With the developed method, SS was directly extracted from the cigarette paper by immersing it in water, and the extracted solution was subsequently detected. The quantitative spike-recoveries were in the range of 95.5–116.7%, with RSD between 2.3–12.6%. The whole detection procedure including the on-site substrate preparation, took only about 30 min. This work opens new avenues for colloidal synthesis, and the detection method of SS on the cigarette paper also holds great promise in food safety applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Zhipeng Zhao,Qian Zhao,Le Zhou,Yi Wei,Biao Lei,Hongwen Zhang,Weiping Cai
Styrene , a prevalent volatile organic compounds (VOCs), is very harmful to atmosphere and humans. Consequently, the development of efficient detection technologies for styrene is of high importance, which is still in challenge! In this work, we crafted a layered double hydroxide (LDH) porous film-coated gold nanoarray, designed to act as a surface enhanced Raman spectroscopy (SERS) chip for the efficient and portable detection of gaseous styrene. This chip features a covering layer composed of cross-linked LDH nanosheets , notable for their porous structure and high specific surface area. When the covering layer is 100–300 nm in thickness, this composite chip has significant enrichment effect and strong SERS performance to gaseous styrene with a lowest detectable concentration below 1 ppb (4.64 ×10 -3 mg/m 3 ), and can response within 10 s, showing the rapid response and high sensitivity. Additionally, the chip has strong anti-interference capabilities and maintains excellent response to styrene, even in mixed benzene-VOCs gases. The exceptional SERS performances of this chip is ascribed to its LDH covering layer-induced styrene-enrichment and structurally-enhanced SERS performances. This study provides a simple route and practical chip for the rapid and ultrasensitive SERS-based detection of gaseous styrene, which is also potentially beneficial for the detection of other gaseous VOCs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
De Zhang,Mingxin He,Chongyang Qin,Zhuoqun Wu,Minhui Cao,Dejiang Ni,Zhi Yu,Pei Liang
The unreasonable spraying and random migration of acetamiprid may cause pollution of crops, soil and water resources in the environment, resulting in threatening ecosystem and human health. However, the monitoring of acetamiprid using mass spectrum in the environment encounters challenges due to high-cost instruments and complex processing time. Herein, we fabricated a rapid and reliable SERS method based on Ag@ZIF-8@Au platforms for tracing acetamiprid residues in the environment. In this method, a MOF material named ZIF-8 is coated with silver nanoparticles and distributed internally between AgNPs and AuNPs to enhance Raman signal, which can enrich pesticide molecules into the hotspots area provided by noble material and helps avoid the oxidation of silver nanoparticles. High sensitivity (LOD of 9.027 × 10 -10 M for acetamiprid, and SERS enhancement factor of 4.3 × 10 7 ), excellent reproducibility (6.496% or 7.198% RSD for 30 random points) and superior stability (3.127% RSD for 6 weeks) were achieved using the proposed method. Acetamiprid with concentrations from 10 -4 to 10 -9 M were successfully detected by SERS method. Furthermore, the linear detection models of acetamiprid in different environment matrices (lake water, tea leaves, tea garden soil, oranges and oranges orchard soil) were established and all the correlation coefficient (R 2 ) were higher than or equal to 95%, indicating the excellent adaptability of Ag@ZIF-8@Au platform in environment. The randomly spiked concentrations of acetamiprid were also tested with good recovery values and low relative error values, further confirming the reliability of the detection method.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 35
·4494-4505
·
IF 3.1
Chao Sun,Lizheng Wang,Naiyu Guo,Runze Hu,Li Ye,Zhiming Hu,Jianjun Ding
Surface-enhanced Raman scattering (SERS) has a unique fingerprint spectrum, which allows for rapid, highly sensitive, and non-destructive detection without the need for sample pretreatment. However, SERS substrates have disadvantages such as short storage time and poor reproducibility. In this study, carbon nanotubes, gold, and silver were combined to take advantage of their inherent structural and characteristic properties that enhance the Raman effect. A new type of SERS composite substrate, CNTs/Ag@Au/SiO2, was prepared using a hydrothermal method and seed growth method. The substrate was characterized using transmission electron microscopy (TEM), and the average distance between the core–shell nanoparticles was found to be 3.1 nm, which is more suitable than other gold–silver combined core–shell structures and significantly improves the SERS enhancement factor. The substrate demonstrated high sensitivity even at low concentrations of probe molecules and good uniformity at five randomly selected locations. After storage for 45 days, the substrate still exhibited good stability. In most gold–silver combined core–shell structures, the detection limit for Rhodamine 6G (R6G) is 10−9 mol L−1, while in this substrate, the detection limit for R6G is 10−11 mol L−1. Furthermore, the contribution of the substrate's enhancement was deeply investigated using finite-difference time-domain (FDTD), which revealed that the substrate's hotspots were present in two forms: the “hotspots generated between Ag@Au nanoparticles” and the “hotspots generated between Ag@Au nanoparticles and carbon nanotubes”. These two forms of hotspots also demonstrated that the performance brought about by the preparation of the substrate structure was reliable. The simulation results were compared with the experimental results, and the analysis showed that the real environment would have an impact on the substrate's structure during the actual substrate preparation process. Finally, the substrate was used for detecting the pesticide fipronil, and the results showed clear peaks even at a concentration of 0.1 mg L−1. The results indicated that the Raman intensity was linearly exponential with the fipronil solution concentration, with a determination coefficient of R2 = 0.991. This study provides a new SERS substrate for pesticide residue detection and further explores the improvement of pesticide detection limits.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
OPTICS EXPRESS
·
2023
·Vol. 31
·期 13
·21025-21037
·
IF 3.8
Houjia Zhang,Zhaoyi Zhang,Hongjun Wang,Lili Huang,Zhenshan Yang,Yangzhi Wang,Yangzhi Wang,Hefu Li,Hefu Li
Flexible surface-enhanced Raman spectroscopy (SERS) substrate has attracted great attention due to its convenient sampling and on-site monitoring capability. However, it is still challenging to fabricate a versatile flexible SERS substrate, which can be used for in situ detection of analytes either in water or on irregular solid surfaces. Here, we report a flexible and transparent SERS substrate based on a wrinkled polydimethylsiloxane (PDMS) film obtained by transferring corrugated structures on the aluminium/polystyrene bilayer film, onto which silver nanoparticles (Ag NPs) are deposited by thermal evaporation. The as-fabricated SERS substrate exhibits a high enhancement factor (∼1.19×105), good signal uniformity (RSD of 6.27%), and excellent batch-to-batch reproducibility (RSD of 7.3%) for rhodamine 6 G. In addition, the Ag NPs@W-PDMS film can maintain high detection sensitivity even after mechanical deformations of bending or torsion for 100 cycles. More importantly, being flexible, transparent, and light, the Ag NPs@W-PDMS film can both float on the water surface and conformally contact with the curved surface for in situ detection. The malachite green in aqueous environment and on apple peel can be easily detected down to 10−6 M with a portable Raman spectrometer. Therefore, it is expected that such a versatile flexible SERS substrate has great potential in on-site, in situ contaminant monitoring for realistic applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Express
·
2023
·Vol. 13
·期 4
·574-583
·
IF 0.7
Li Xiaochun,Li Guanli,Wu Mei,Pang Yongfeng,Nie Hui,Wu Shujie,Tang Xiaoxian,Luo Yanghe,Li Xiaolong
Gold-doped carbon dots (CDAu) were simply and quickly prepared via microwave procedure using fullerene as a precursor, a rapid detection method for chlorpyrifos was established using surface enhanced Raman scattering (SERS) combined with aptamer (Apt) controlled gold-doped
carbon dots catalytic reaction. As the result, the SERS intensity decreased due to the aptamer inhibited the CDAu catalytic reduction of chlorauric acid by glucose. However, in the presence of chlorpyrifos, SERS intensity increased linearly with the concentration of chlorpyrifos
in the range of 12.8–432.8 ng/L, the correlation coefficient was R
2 = 0.9961.
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Jiwei Wang,Jingjing Fu,Han Chen,Ali Wang,Yuting Ma,Hanrong Yan,Yuting Li,Dehong Yu,Fenglei Gao,Shibao Li
Accurate quantitative, in situ and temporal tracking imaging of tumor-associated miRNAs in living cells could provide a basis for cancer diagnosis and prognosis. In this strategy, a surface-enhanced Raman scattering (SERS)-fluorescence (FL) dual-spectral sensor (DSS) was constructed based on the nanoscale photophysical properties of AuNPs , mediated by functionalized DNA , to achieve rapid imaging of FL and accurate SERS quantification of intracellular miRNAs. The dual-spectrum sensor in the strategy is highly sensitive, specific and reproducibly stable. The LOD values of the dual spectra were 3.58 pM (SERS) as well as 11.8 pM (FL) with RSD values less than 2.69%. The bispectral sensor self-assembled into a trimer by the lapidation of Y-type DNA under the excitation of the target, generating a stable enhanced electric field coupling; and selected adenine located in the enhanced electric field as the reporter molecule, simplifying the labeling process and variables of the Raman reporter molecule, distinguishing it from other traditional methods. This strategy successfully achieved accurate tracking and quantification of miR-21 in cancer cells and showed good stability in the cells. The reported probes are potential tools for reliable monitoring of biomolecular dynamics in living cells.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 20
·4915-4922
·
IF 3.864
Ya-Fei Tian,Cui-Fang Ning,Fang He,Bin-Cheng Yin,Bang-Ce Ye
Exosomes, as important signal transmitters, play a key role in intercellular communication, especially in cancer metastasis. There is considerable evidence that exosomes can be used as an indicator of cancer. However, convenient and sensitive methods for detecting exosomes are still technically challenging. Here, we present a convenient and highly sensitive surface-enhanced Raman scattering (SERS) based method by combining immunoaffinity, SERS nanoprobes, and portable Raman devices for specific isolation and accurate quantification of exosomes. To construct the SERS-based biosensor, the surfaces of gold nanostar@4-mercaptobenzoic acid@nanoshell structures (AuNS@4-MBA@Au) are modified with a bivalent cholesterol (B-Chol)-labeled DNA anchor to prepare SERS nanoprobes. Exosomes are specifically captured by immunomagnetic beads, and then SERS nanoprobes are fixed on the surface of exosomes by hydrophobic interactions between cholesterol and lipid membranes, thus forming a sandwich-type immunocomplex. The immunocomplex can be magnetically captured and produce enhanced SERS signals. In the absence of exosomes, the sandwich-type immunocomplex cannot be formed, and thus negligible SERS signals are detected. The degree of immunocomplex assembly and the corresponding SERS signals are positively correlated with the exosome concentration over a wide linear range of 40 to 4 × 107 particles per μL and the limit of detection is as low as 27 particles per μL. Consequently, a sensitive and simple strategy for detection of exosomes is successfully constructed. We believe that our biosensor has considerable potential as a convenient and highly sensitive quantification tool to detect exosomes in biological samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Research
·
2019
·Vol. 12
·期 6
·1375-1379
·
IF 8.515
Xu Meng,Tu Guopeng,Ji Muwei,Wan Xiaodong,Liu Jiajia,Liu Jia,Rong Hongpan,Yang Yanlian,Wang Chen,Zhang Jiatao
Utilizing vacuum-tuned-atmosphere induced dip coating method, we achieve the cross-dimensional macroscopic diverse self-assemblies by using one building block with one chemical functionality. Coordinated modulating the vacuum degree, colloid concentration and evaporation atmosphere, Au@Ag core/shell NCs can controllably assemble into diverse multi-dimensional superstructures. Under 0.08 MPa, we obtained the two-dimensional (2D) stepped superstructures with continuously tunable step width. In addition, we generated a series of tailorable nanoscale-roughened 2D Au@Ag NCs superstructures at 0.04 MPa, which exhibited the label-free ultrasensitive SERS detection for the different mutants of IAPP8-37 proteins. Under 0.01 MPa, we obtained the cross-dimensional tailorable Au@Ag NCs assemblies from random to macroscale 2D and three-dimensional (3D) densest superstructures by adjusting the capping ligand-environmental molecule interactions. This is a flexible method to generate as-prepared Au@Ag core/shell NCs into well-defined macroscopic diverse superstructures and to promote the exploitation into biological applications.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）