Biomedical Materials
·
2020
·Vol. 16
·期 1
·015004
·
IF 3.174
Chunxuan Wu,Zehao Yu,Yihan Li,Kui Zhou,Chuanliang Cao,Peng Zhang,Wenchao Li
Wound healing is a dynamic and well-orchestrated process that can be promoted by creating an optimal environment with wound dressing. An ideal wound dressing material should possess a suitable matrix, structure and bioactive components, functioning synergistically to accelerate wound healing. Wound dressings that allow reproducibility and customizability are highly desirable in clinical practice. In this study, using chitosan (CS) as the matrix and bioglass (BG) as the biological component, a spatially designed dressing scaffold was fabricated from a home-made cryogenic printing system. The micro- and macro-structures of the scaffold were highly controllable and reproducible. The printed scaffold exhibited interconnected and hierarchical pore structures, as well as good flexibility and water absorption capacity, and these properties were not affected by the content of BG. Nevertheless, when the content of BGs exceeded 20% that of CS, the tension strength and elongation rate reduced, but in vitro antibacterial, cell proliferation and migration performance were enhanced. In vivo examinations revealed that the composite scaffold significantly promoted wound healing process, with the group having 30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups. These results indicate that the 3D printed CS/BG composite scaffold is a promising dressing material that accelerates wound healing.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yaqi Zhang,Hongliang Wei,Bingya Hua,Chunwang Hu,Wenjing Zhang
Chitosan based hydrogels with multiple stimulus responses have broad application prospects in many fields. Considering the advantages of semi interpenetrating network (IPN) technology and the special temperature and ion responsiveness of polymers containing zwitterionic groups, a semi-IPN hydrogel was prepared through in situ free radical polymerization of N , N -dimethyl acrylamide and [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl) ammonium hydroxide with polyethylene glycol dimethacrylate as a crosslinker and carboxymethyl chitosan as filler. The gel mass fraction and swelling ratio were measured, and the preparation conditions were optimized. The result indicated that the hydrogel possessed a unique thermo-/pH−/ ion-sensitive behavior. The swelling ratio increased with the increase of temperature and ion concentration, and showed a decreasing trend with the increase in pH. In addition, the hydrogel was stable when the stimuli changed. Adsorption behavior of the hydrogel to Eosin Y (EY) was systematically investigated. The adsorption process can be described well by the pseudo-second-order kinetic model and Langmuir isotherm model, indicating that it was a chemical adsorption. The experiments indicated that the hydrogel exhibited good antifouling and reusability features. Therefore, the semi-IPN hydrogel with antifouling properties and thermo-/pH−/ion-sensitivity can be easily manufactured is expected to find applications in water treatment fields.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Pei Li,Tao Xu,Xuan Dang,Lu Shao,Linlin Yan,Xiaobin Yang,Lexun Lin,Liping Ren,Rong Song
Periodontitis is a chronic inflammatory disease primarily driven by host inflammation and plaque-induced immune responses. Controlling the host inflammatory response and improving the periodontal inflammatory microenvironment are crucial to promoting periodontal tissue regeneration . In this study, the blended nanofiber membranes previously prepared by our research group were improved, and we developed multifunctional chitosan/polyvinyl alcohol/graphene oxide/astaxanthin coaxial nanofiber membranes. Scanning electron microscopy showed that the prepared nanofibers had a smooth surface and a uniform diameter distribution. The mechanical property test results showed that the coaxial nanofiber membranes exhibited higher tensile strength compared to the blended nanofiber membranes, which increased from 4.50 ± 0.32 and 3.70 ± 0.45 MPa to 7.12 ± 0.22 and 5.62 ± 0.79 MPa respectively. Drug release studies indicated that the “shell-core” structure of coaxial nanofibers significantly reduced the initial burst release of astaxanthin (ASTA), with only 13.49 % and 10.71 % release in the first 24 h, and drug release lasted for over a week. Animal experiments confirmed that the coaxial nanofiber membranes loaded with ASTA promoted periodontal bone defect repair while inhibiting periodontal inflammation. In conclusion, the prepared coaxial nanofiber membranes are a promising sustained-release drug system for treating periodontitis.
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POLYMER
·
2023
·
IF 4.6
Hongnan Zhang,Mengting Cui,Tingting Zhang,Xiaohong Qin
Traditional textiles are conductive to breed germs that might cause infection or unpleasant odor, especially in public hygiene products. Moreover, biosafety, environmental protection and cost are crucial considerations for antimicrobial materials. Herein, we developed a biocompatible microcapsules/nanofibrous membrane using eco-friendly and facile strategy. The microcapsules loaded with the Epigallocatechin and gallate (EGCG) were synthesized through an ion-crosslinking reaction between chitosan (CS) and sodium tripolyphosphate (TPP). Additionally, the addition of octadecyl trimethyl ammonium chloride (STAC) could further enhance the antibacterial activity . Following that, the microcapsules were combined with the polycaprolactone (PCL) nanofibers . The resultant composite nanofibrous membrane exhibits a slow release effect, an enjoyable deodorant activity, and strengthened antibacterial activity through contact-killing and biocide-releasing. Besides, the membrane behaved a low degradation rate , without compromising its mechanical and hydrophilic properties after a 20-day degradation. These findings provided a biocompatible and practical approach for antimicrobial and odor-proof material for hygienic and sanitary products.
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Scientific Reports
·
2023
·Vol. 13
·期 1
·1-15
·
IF 4.6
Zheng Xiaoran,Gao Min,Wu Liangquan,Lu Xin,Lin Qiuqi,Zhong Hai,Lu Yingfei,Zhang Yunlei,Zhang Xiuwei
Pseudomonas aeruginosa ( P. aeruginosa ) infections present a grave threat to immunocompromised individuals, particularly those with cystic fibrosis due to the development of bacterial biofilms. In this study, we engineered self-assembling chitosan-ceftazidime nanoparticles (CSCE) capable of effectively penetrating biofilms and eradicating P. aeruginos a. The CSCE nanoparticles were synthesized through ionic cross-linking, combining negatively charged ceftazidime with positively charged chitosan, resulting in uniform nanoparticles measuring approximately 40 nm in diameter, exhibiting high dispersity and excellent biocompatibility. Remarkably, these nanoparticles exhibited significant inhibition of P. aeruginosa growth, reduced pyocyanin production, and diminished biofilm formation, achieving a maximum inhibition rate of 22.44%. Furthermore, in vivo investigations demonstrated enhanced survival in mice with abdominal P. aeruginosa infection following treatment with CSCE nanoparticles, accompanied by reduced levels of inflammatory cytokines Interleukin-6 (125.79 ± 18.63 pg/mL), Interleukin-17 (125.67 ± 5.94 pg/mL), and Tumor Necrosis Factor-α (135.4 ± 11.77 pg/mL). Critically, mice treated with CSCE nanoparticles showed no presence of bacteria in the bloodstream following intraperitoneal P. aeruginosa infection. Collectively, our findings highlight the potential of these synthesized nanoparticles as effective agents against P. aeruginosa infections.
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Frontiers in Pharmacology
·
2023
·
IF 5.6
Yulong Chen,Qingzhou Cheng,Shan Zeng,Site Lv
Background: Migraine is a common neurovascular disorder with typical throbbing and unilateral headaches, causing a considerable healthcare burden on the global economy. This research aims to prepare chitosan-alginate (CS-AL) nanoparticles (NPs) containing Foshousan oil (FSSO) and investigate its potential therapeutic effects on the treatment of migraine. Methods: FSSO-loaded CS-AL NPs were prepared by using the single emulsion solvent evaporation method. Lipopolysaccharide (LPS)-stimulated BV-2 cells and nitroglycerin (NTG)-induced migraine mice were further used to explore anti-migraine activities and potential mechanisms of this botanical drug. Results: FSSO-loaded CS-AL NPs (212.1 ± 5.2 nm, 45.1 ± 6.2 mV) had a well-defined spherical shape with prolonged drug release and good storage within 4 weeks. FSSO and FSSO-loaded CS-AL NPs (5, 10, and 15 μg/mL) showed anti-inflammatory activities in LPS-treated BV-2 cells via reducing the levels of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and nitric oxide (NO), but elevating interleukin-10 (IL-10) expressions. Moreover, FSSO-loaded CS-AL NPs (52 and 104 mg/kg) raised pain thresholds against the hot stimulus and decreased acetic acid-induced writhing frequency and foot-licking duration in NTG-induced migraine mice. Compared with the model group, calcitonin gene-related peptide (CGRP) and NO levels were downregulated, but 5-hydroxytryptamine (5-HT) and endothelin (ET) levels were upregulated along with rebalanced ET/NO ratio, and vasomotor dysfunction was alleviated by promoting cerebral blood flow (CBF) in the FSSO-loaded CS-AL NPs (104 mg/kg) group. Conclusion: FSSO-loaded CS-AL NPs could attenuate migraine via inhibiting neuroinflammation in LPS-stimulated BV-2 cells and regulating vasoactive substances in NTG-induced migraine mice. These findings suggest that the FSS formula may be exploited as new phytotherapy for treating migraine.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Jingmin Cui,Yan Sun,Linqing Wang,Wenqiang Tan,Zhanyong Guo
In this study, nine chitosan derivatives containing aromatic five-membered heterocycles were prepared and the effects of different grafting methods on the biological activities of chitosan derivatives were investigated. The structures of all the compounds were characterized by Fourier Transform Infrared (FT-IR) spectroscopy and Nuclear Magnetic Resonance (NMR) spectroscopy, while the antioxidant, antifungal and antibacterial activities of the chitosan derivatives were tested. The experimental data suggested that the chitosan derivatives had outstanding inhibitory ability against Fusarium graminearum , Fusarium oxysporum f . sp . cucumbrum , Staphylococcus aureus and Escherichia coli . At the same time, some of the compounds showed strong scavenging ability against DPPH radical and superoxide radical. Cytotoxicity experiments have demonstrated that some chitosan derivatives are non-toxic to L929 cells. More importantly, compared to chitosan, these chitosan derivatives have good water solubility and can be used as potential polymers for antifungal and antibacterial biomaterials in agriculture.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Yongli Shi,Jie Feng,Zhaoxin Zhang,Ningning Cao,Jiahao Li,Heping Li,Linji Li,Quanxian Hua,Qun Ma,Kai Zhang
Polypyrrole-based adsorbents have emerged in removing Cr(VI) from wastewater based on their reduction, chelation, electrostatic attraction and ion exchange. However, it is still a formidable challenge to remove metal cations simultaneously due to electrostatic repulsion. In fact, metal ions originating from industrial activities such as chemical manufacturing and metal electroplating are always complex and multifarious. Here, we report a EDTA-crosslinking-chitosan/polypyrrole composite (EDTA-CS/PPy) with multiple types of adsorption sites (carboxyl, hydroxyl, amino), successfully realizing simultaneous removal of Cr(VI) anions and other metal cations. The maximum adsorption capacities of Cr(VI), Cu(II), Pb(II) and Cd(II) reach up to 401.25, 59.03, 77.81 and 68.31 mg/g, respectively. The adsorption mechanism is attributed to coupling interactions of the reduction, chelation, electrostatic attraction and ion exchange between targeted metal ions and EDTA-CS/PPy, investigated by experiments and DFT theories. Notably, the adsorption capacity of EDTA-CS/PPy keeps stability after three adsorption–desorption cycles. These findings may promote potential applications of EDTA-CS/PPy adsorbents in highly efficient treatment of coexisting toxic metal ions from wastewater.
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EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2023
·
IF 4.6
Yingqiao Li,Zhiru Zou,Jinyu An,Xiaoyao Liu,Qian Wu,Junpeng Sun,Xiaobang Liu,Jiaqun Du,Ying Xiong,Chao Wu,Xifan Mei,He Tian
Spinal cord injury (SCI) is a central system disease with a high rate of disability. Pathological changes such as ischemia and hypoxia of local tissues, oxidative stress and apoptosis could lead to limb pain, paralysis and even life-threatening. It was reported that catalase (CAT) was the main antioxidant in organisms, which could remove reactive oxygen species (ROS) and release oxygen (O 2 ). However, the efficacy of the drug is largely limited due to its poor stability, low bioavailability and inability to cross the blood spinal cord barrier (BSCB). Therefore, in this study, we prepared folic acid-functionalized chitosan nanoparticles to deliver CAT (FA-CSN CAT) for solving this problem. In vivo small animal imaging results showed that FA-CSN could carry CAT across the BSCB and target to the inflammatory site. In addition, Immunofluorescence, ROS assay and JC-1 probe were used to detect the therapeutic effect of FA-CSN CAT in vitro and in vivo. The results showed that FA-CSN CAT could alleviate the hypoxic environment at the injured site and remove ROS, thereby inhibiting oxidative stress and protecting neurons, which may provide a new idea for clinical medication of SCI.
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CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Yongsheng Li,Chengnan Chu,Chuntao Chen,Bianjing Sun,Jingjing Wu,Shujun Wang,Weiwei Ding,Dongping Sun
Management of noncompressible torso hemorrhage is an urgent clinical requirement, desiring biomaterials with rapid hemostasis , anti-infection and excellent resilient properties. In this research, we have prepared a highly resilient cryogel with both hemostatic and antibacterial effects by chemical crosslinking and electrostatic interaction . The network structure crosslinked by quaternized chitosan and genipin was interspersed with oxidized bacterial cellulose after lyophilization. The as-prepared cryogel can quickly return to the original volume when soaking in water or blood. The appropriately sized pores in the cryogel help to absorb blood cells and further activate coagulation, while the quaternary ammonium salt groups on quaternized chitosan inhibit bacterial infections. Both cell and animal experiments showed that the cryogel was hypotoxic and could promote the regeneration of wound tissue. This research provides a new pathway for the preparation of double crosslinking cryogels and offers effective and safe biomaterials for the emergent bleeding management of incompressible wounds.
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COLLOID AND POLYMER SCIENCE
·
2023
·Vol. 301
·期 12
·1475-1486
·
IF 2.4
Li Yinglu,Zhao Jing,Wang Yurui,Zhao Tianfu,Song Yanan,Liang Fei
Nanoscale metal organic frameworks (NMOFs) have become excellent drug carriers for the treatment of cancer or other diseases, due to the advantages of large surface area, high drug loading, tunable structure and easy functionalization, with potential application in the field of drug delivery. In this paper, UiO-66 NMOF was synthesized using terephthalic acid as organic ligand and zirconium chloride as metal salt, and then biocompatible chitosan (CS) and PEGylated chitosan (CS-g-mPEG)-decorated NMOFs as drug carriers were constructed by surface modification, using 5-FU as the drug model. The structures, size and morphology were characterized by IR, XRD, DLS, SEM and TEM respectively. The results showed that when the concentration was 0.0872 M, the molar ratio was 1:1 and the acetic acid amount was 2.9 mL, the average particle size of NMOFs was 145.60 nm and 171.73 nm, respectively. The drug loading capacity and encapsulation rate could reach 15.7% and 52.8%. The in vitro controlled drug release results indicated that the release rate accelerated with increasing temperature and decreasing initial amount, greatly reduced under acidic conditions, and the coating of CS and CS-g-mPEG on the surface of UiO-66 had the effect of controlling drug release, further improved the bioavailability of 5-FU. The decorated UiO-66 NMOFs show excellent biocompatibility as nanodrug carriers and can avoid rapid degradation under gastric acid, which are more suitable for oral drug delivery.
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Carbon Letters
·
2023
·Vol. 33
·期 6
·1661-1667
·
IF 4.5
Tian Tingyu,Cai Yuping,Yang Shimao,Guo Yanwei,Zhou Wei
In this study, we successfully grafted chitosan (CS) onto multi-walled carbon nanotubes (MWCNTs) to enhance their properties and potential applications in the biomedical field. FTIR spectroscopy confirmed the successful covalent bonding of CS onto MWCNTs, indicated by the new absorption peak of the amide bond (–CONH–). Thermal analysis showed that the modified MWCNTs (MWCNT-CS) had significant weight loss around 260 °C, suggesting the decomposition of hydroxypropyl chitosan, and confirming its presence in the nanocomposite. SEM images revealed that CS grafting improved the dispersibility of MWCNTs, a property crucial for their use as nanofillers in polymers. Moreover, the micro-tensile bond strength of dentin surface increased with increasing MWCNT-CS concentrations, indicating the potential of MWCNT-CS as a pretreatment for dentin bonding. After simulated aging, the bond strength remained significantly higher for MWCNT-CS groups compared to those without pretreatment. In biocompatibility assessment using the MTT assay, MWCNT-CS showed higher cell viability than MWCNT, suggesting improved biocompatibility after CS modification. The results of this study suggest that CS-modified MWCNTs could be promising materials for applications in dentin bonding, dentin mineralization, bone scaffolding, implants, and drug delivery systems.
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