SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Tingting Luo,Yiqi Li,Yanxue Xu,Shiting Zhang,Yujue Wang,Xingming Kou,Dan Xiao
In this article, a hyperfluorescence 2-pyridone derivative (D-TPCA) has been synthesized within 5 min by the microwave-assistant heat treatment of the aqueous mixture of citric acid and 2-aminoethanethiol hydrochloride. The as-prepared D-TPCA exhibited good aqueous solubility and excellent fluorescent property with a quantum yield of 89.9%. Because the fluorescence of D-TPCA could be effectively quenched by picric acid (PA), D-TPCA could be served as a “turn-off” fluorescence sensor for PA in aqueous solution. The proposed sensing method permitted a selective and sensitive detection of PA in a linear range of 0–45 μM with a detection limit of 56.0 nM. The determination results of PA in real water samples with a recovery range of 95.4–107.3% confirmed a good analytical performance of this sensor. In addition, the possible sensing mechanism has been discussed in detail.
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SENSORS AND ACTUATORS B-CHEMICAL
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2020
·
IF 7.1
Wenxi Xia,Shuangzhe Zhang,Jiangli Fan,Yueqing Li,Xiaojun Peng
Combining imaging and treatment of cancer into one molecule has become an efficient strategy for studies on pathology and cancer therapeutics. Herein, a novel highly selective and sensitive green-emitting probe ( APLN ), which contained a fluorophore (Naphthalimide), a linker, and a COX-2 kinase inhibitor (aspirin) has been designed and evaluated. Cyclooxygenase-2 (COX-2), as one of the typical rate-limiting enzymes, has been utilized as imaging and therapeutic target since this enzyme is overexpressed in various cancer cell lines. Aspirin, a non-steroidal anti-inflammatory drug (NSAID), has been reported to reduce the incidence and risk of cancers by down-regulating the expression of COX-2 in cancer cells. The APLN probe has been proved to successfully label the COX-2 enzyme to identify tumors and significantly inhibit tumor growth in a xenotransplantation model of nude mice. Therefore, this enzyme-targeted fluorescence probe ( APLN) is a potential reagent for in situ imaging and fluorescent-visible cancer therapy.
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FEMS MICROBIOLOGY LETTERS
·
2015
·Vol. 362
·期 6
·
IF 2.121
Zhao Jianqing,Pan Shan,Lin Lan,Fu Lei,Yang Chao,Xu Zhongmin,Wei YanMin,Jin Meilin,Zhang Anding
Streptococcus suis (S. suis) ranks among the five most important porcine pathogens worldwide and occasionally threatens human health, especially in people that come into close contact with pigs or pork products. Streptococcus suis serotype 2 (SS2) is considered to be the most pathogenic and prevalent capsular type. As a first line of immune defense against SS2 infection, neutrophils can eliminate the invader not only by phagocytosis but also by neutrophil extracellular traps (NETs)-mediated killing. SS2 can resist phagocytosis through polysaccharide capsule (CPS), but how this strain evades the effects of NETs remains to be determined. The present study demonstrated that the epidemic strain 05ZY, the highly pathogenic strain P1/7 and the intermediately pathogenic strain A7 could induce the formation of NETs. Furthermore, SS2 strains could successfully resist NETs-mediated killing, and the CPS structure contributed to this resistance by escaping the trapping. Therefore, the CPS structure not only contributed to the SS2 strains’ resistance to phagocytosis-mediated killing but also played an essential role in evading NETs trapping and further killing in vitro. This study strengthens our understanding of how S. suis can evade innate immune surveillance and elimination.
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PubMed
高影响因子
CELL PROLIFERATION
·
2022
·Vol. 55
·期 6
·e13252
·
IF 8.755
Xiaoyi Lin,Daoming Zhu,Kaiyang Wang,Pengbo Luo,Gang Rui,Youshui Gao,Fuan Liu,Hongping Yu
Objectives Osteonecrosis of the femoral head (ONFH) is a devastating disease characterized by destructive bone structures, enlarged adipocyte accumulation and impaired vascularization. The aldehyde dehydrogenase 2 (ALDH 2) is the limiting enzyme for ethanol metabolism with many physiological functions. The aim was investigated the potential protective role of activated ALDH 2 by Alda-1 for ethanol-induced ONFH. Materials and Methods The ethanol-induced ONFH in rat was performed to explore the protective of Alda-1 by various experimental methods. Subsequently, the effect of Alda-1 and ethanol on the osteogenic and adipogenic differentiation was investigated via multiple cellular and molecular methods. Finally, the effect of Alda-1 and ethanol on the neo-vascularization was detected in Human umbilical vein endothelial cells (HUVECs) and ONFH model. Results Firstly, radiographical and pathological measurements indicated that alda-1 protected ethanol-induced ONFH. Moreover, ethanol significantly inhibited the proliferation and osteogenic differentiation of BMSCs, whereas Alda-1 could distinctly rescue it by PI3K/AKT signalling. Secondly, ethanol remarkably promoted the lipid vacuoles formation of BMSCs, while Alda-1 significantly retarded it on BMSCs by AMPK signalling pathway. Finally, ethanol significantly inhibited proliferation and growth factor level resulting in reduced angiogenesis, whereas Alda-1 could rescue the effect of ethanol. Additionally, Alda-1 significantly reduced the occurrence of ONFH and promoted vessel number and distribution in alcoholic ONFH. Conclusions Alda-1 activation of ALDH 2 was highly demonstrated to protect ethanol-induced ONFH by triggering new bone formation, reducing adipogenesis and stimulating vascularization.
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PubMed
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MEDICAL SCIENCE MONITOR
·
2018
·
IF 1.894
Shishan Xiao,Hongqian Zhu
Background Leukemia cells have strong proliferation and anti-apoptosis capabilities. The purpose of this study was to investigate the effect of silencing the leucine-rich alpha-2-glycoprotein1 (LRG1) gene, which was found to regulate tumor proliferation and apoptosis in acute myeloid leukemia (AML) cell lines. Material/Methods Plasmid interference technique was used to silence the LRG1 gene in the KASUMI-1 cell line. The cell counting kit-8 (CCK-8) assay was used to test the effect of transduction on cell viability. Cell cycle and apoptosis were detected by flow cytometry. Western blot and quantitative real-time polymerase chain reaction (RT-qPCR) were applied to detect the expression levels of proteins and mRNA, respectively. Results KASUMI-1 cells with the CD34 + CD38 − phenotype were sorted by flow cytometry. After transfection of the siLRG1 plasmid, the level of LRG1 expression was downregulated and cell viability was reduced. Silencing of LRG1 gene blocked KASUMI-1 cells in G0/G1 phase and promoted apoptosis. Further experiments found that LRG1 gene silencing significantly downregulated cell cycle-associated proteins and anti-apoptotic proteins, while upregulating pro-apoptotic proteins. Downregulation of LRG1 gene expression also inhibits signal transduction of the JAK-STAT pathway. Conclusions LRG1 gene silencing regulates the expression of cyclin and apoptosis-related proteins to reduce cell viability and promote apoptosis, probably through inhibition of the JAK-STAT pathway.
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PubMed
高影响因子
ChemMedChem
·
2017
·Vol. 12
·期 4
·327-336
·
IF 3.225
Zixin Xie,Zaikui Zhang,Shufang Yu,Donghua Cheng,Huan Zhang,Chao Han,Handeng Lv,Faqing Ye
Graphical Taking the heat off : The 24 curcumin analogues in this study were found to have no toxicity toward mouse bone marrow cells, and some of them showed potent anti-inflammatory activity by inhibiting LPS-stimulated TNFα, IL-6, IL-1β, PGE2, and NO production in rat macrophages. Pharmacokinetic studies indicated that compound c6 has better bioavailability than curcumin in vivo. Both c6 and c10 may be valuable leads for the development of new anti-inflammatory agents. A total of 24 N-substituted 3,5-bis(2-(trifluoromethyl)benzylidene)piperidin-4-one derivatives were synthesized via aldol condensation, and their anti-inflammatory activities were evaluated. These compounds were found to have no significant cytotoxicity against mouse bone marrow cells in vitro. However, some compounds, such as c6 ( N -(3-methylbenzoyl)-3,5-bis-(2-(trifluoromethyl)benzylidene)piperidin-4-one) and c10 ( N -(2-chlorobenzoyl)-3,5-bis-(2-(trifluoromethyl)benzylidene)piperidin-4-one), displayed potent anti-inflammatory activity by inhibiting lipopolysaccharide (LPS)-stimulated tumor necrosis factor (TNF)-α, interleukin-6 (IL-6), IL-1β, prostaglandin E2 (PGE2), and nitric oxide (NO) production in RAW 264.7 cells. Treatment with c6 or c10 at 2.5 or 10 mg kg −1 significantly decreased the paw edema induced by carrageenan in rats, and the anti-inflammatory effects of these compounds were found to be better than those of celecoxib or indomethacin as well as their parent compound C66 (2,6-bis-(2-(trifluoromethyl)benzylidene)cyclohexanone). Pharmacokinetic analysis indicated that c6 has better bioavailability than curcumin. Therefore, these compounds may be valuable leads for the development of new anti-inflammatory drugs.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Fei Li,Yunlu Wei,Jing Zhao,Guoyong Yu,Linlin Huang,Quanhong Li
Pumpkin polysaccharides with various bioactivities are mainly taken orally, thus detailed knowledge of the intestinal transport of which are essential for understanding its bioactivities. The Caco-2 cells monolayer model (mimic intestinal epithelium) was successfully constructed and Cucurbia moschata polysaccharides (PPc-F) were successfully conjugated with fluorescein isothiocyanate (FITC) to evaluate the transcellular transport mechanism and subcellular localization of PPc. The transport process of PPc-F was energy-dependent, and a moderately-absorbed biological macromolecule according to the apparent permeability coefficients (P app ) value. The endocytosis process of PPc-F in Caco-2 cells included the clathrin- and caveolae (or lipid draft)-medicated routes. And the translocation process was related to endoplasmic reticulum (ER), golgi apparatus (GA), tubulin and the acidification of endosomes. As for the intracellular location of PPc-F, it was mainly accumulated in ER. The study provided an understanding of the transmembrane transport of PPc-F, and could help studying the mechanisms of its effects.
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ACS Omega
·
2018
·
IF 0
Yuanzi Wu,Chao Yan,Jian’an He,Wenli Xiong,Shuigen Wu,Shutao Liu,Zhen Cai
Capsid protein of porcine circovirus type 2 (PCV2) is an ideal subunit vaccine candidate for the postweaning multisystemic wasting syndrome. In this study, mannan-mediated targeting of PCV2ΔCap42-233 protein to antigen presenting cells (APCs) was investigated for the development of PCV2 vaccine. Mannan was attached to PCV2ΔCap42-233 protein via an acid sensitive Schiff base reaction. The mannosylated protein was endowed with the capacity to target the mannose receptor on APCs as well as the ability of controlled release of the antigen in the acidic condition of the lysosome. Finally, the immune response of mannosylated PCV2ΔCap42-233 protein in mice was evaluated. The mannosylated protein exhibited the ability to stimulate humoral immune response and enhance the immunity. Thus, acid-sensitive and APCs-targeting mannosylated PCV2ΔCap42-233 protein represents a promising candidate for the potential commercial application as an efficient vaccine against porcine circovirus.
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Bioresources and Bioprocessing
·
2019
·Vol. 6
·期 1
·1-12
·
IF 0
Zhang Can,Song Wei,Liu Jia,Chen Xiulai,Liu Liming
( R )- or ( S )-2-Hydroxy-4-(methylthio)butanoic acid (HMTBA) is used as a poultry nutritional supplement and to treat renal failure disease. Herein, we report an artificially designed biocatalytic cascade process, which uses l -methionine to synthesize ( R )- or ( S )-HMTBA. This biocatalysis cascade comprises a basic module and two different extender modules and operates in a modular assembly manner. The basic module responsible for the transformation of l -methionine to α-keto-γ-methylthiobutyric acid (KMTB) is comprised of the l -amino acid deaminase. Two different extender modules responsible for the transformation of KMTB to ( R )- or ( S )-HMTBA are comprised of the R/S-specific lactate dehydrogenase in combination with the formate dehydrogenase, respectively. Engineered Escherichia coli catalysts, one containing the basic module, the other containing the one of two different extender modules, produced 97.6 g L −1 ( R )-HMTBA and 96.4 g L −1 ( S )-HMTBA with a yield of 96.9% and 95.8% at the large scale (1 L) using a two-stage strategy in one pot, respectively. Therefore, this biocatalytic process lays the foundation for the industrial-scale conversion of low-cost l -amino acids to corresponding high-value enantiopure chiral 2-hydroxy acids.
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FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Yilun Luo,Wei Zheng,Qian Shen,Li Zhang,Cuie Tang,Rong Song,Shilin Liu,Bin Li,Yijie Chen
To investigate the adsorption kinetics and dilatational rheological properties of Pea Albumin-2 (PA-2) at the oil-water interface, recombinant PA-2 was expressed and purified. Our native-PAGE, SDS-PAGE, and homology modeling results indicated that PA-2, a kind of globular protein, could form dimers by non-covalent interactions rather than disulfide bonds. The TEM results showed that some PA-2 proteins could form aggregates with the size of around 2–3 μm. Unlike other globular proteins, PA-2 exhibited the non-monotonic kinetic dependency of the dynamic viscoelastic modulus (E). Besides, the rate of rearrangement ( k R ) of PA-2 was significantly higher than that of other typical food proteins. Our results demonstrated that PA-2 could adsorb to the oil-water interface in the form of aggregates. The addition of 2-ME (β-mercaptoethanol) would impact dilatational rheological properties of PA-2, leading to disaggregation and higher rearrangement of PA-2 protein aggregates at the oil-water interface. Our data offered more information on the interfacial dynamic properties of globular proteins, and provided a new insight to the conformational changes of globular proteins at the oil-water interface, basing on their dynamic viscoelastic behaviors.
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APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Guomin Geng,Mengyang Cai,Rui Fang,Qian Luan,Zhiqiang Zhang,Jianjun Song,Jian Zhang
Developing highly efficient and stable catalysts toward advanced oxidation technology is the key to achieve industrial peroxymonosulfate (PMS) activation for pollution degradation. Perovskite-type oxide catalysts show high activity and stability for PMS activation, yet the small specific surface area of perovskite catalysts limits the increase of catalytic performance. Metal-organic frameworks (MOFs) have been widely used as the templates/precursors to prepare high specific surface area nanomaterials. In this study, two kinds of high surface area perovskites LaCoO 3 and LaMnO 3 were firstly fabricated with MOFs as templates. Benefiting from the extraordinary skeleton structure of MOFs, well-defined architecture and porous perovskite catalysts show superior catalytic performance towards PMS activation for the degradation of 2, 4-dichlorophenol (2, 4-DCP), with a 99.8% degradation of 2, 4-DCP in 25 min for LaCoO 3 catalyst. Moreover, the LaCoO 3 catalysts exhibit long-term stability with a slight loss of catalytic activity during 3 times recycled experiments. This study not only developed high activity catalysts for PMS activation, but also expanded the MOFs applications for environment and energy.
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高影响因子
MOLECULES
·
2022
·Vol. 27
·期 12
·3669
·
IF 4.927
Yi Wang,Xiangli Zhang,Wenya Zhuang,Yanlei Yu,Xuanrong Sun,Hong Wang,Fengzhi Li,Qingyong Li
Irinotecan and Topotecan are two Camptothecin derivatives (CPTs) whose resistance is associated with the high expression of breast cancer resistance protein (BCRP) and P-glycoprotein (P-gp). To reverse this resistance, two novel CPTs, FL77-28 (7-(3-Fluoro-4-methylphenyl)-10,11-methylenedioxy-20(S)-CPT) and FL77-29 (7-(4-Fluoro-3-methylphenyl)-10,11-methylenedioxy-20(S)-CPT), were synthesized by our group. In this study, the anti-tumor activities of FL77-28, FL77-29, and their parent, FL118 (10,11-methylenedioxy-20(S)-CPT), were evaluated and the results showed that FL77-28 and FL77-29 had stronger anti-tumor activities than FL118. The transport and uptake of FL118, FL77-28, and FL77-29 were investigated in Caco-2 cells for the preliminary prediction of intestinal absorption. The apparent permeability coefficient from apical to basolateral (Papp AP-BL) values of FL77-28 and FL77-29 were (2.32 ± 0.04) × 10−6cm/s and (2.48 ± 0.18) × 10−6cm/s, respectively, suggesting that the compounds had moderate absorption. Since the transport property of FL77-28 was passive diffusion and the efflux ratio (ER) was less than 2, two chemical inhibitors were added to further confirm the involvement of efflux proteins. The results showed that FL77-28 was not a substrate of P-gp or BCRP, but FL77-29 was mediated by P-gp. In conclusion, FL77-28 might be a promising candidate to overcome drug resistance induced by multiple efflux proteins.Keywords:Camptothecin derivatives;cytotoxicity;bioavailability;Caco-2 cells model;drug resistance
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