EUROPEAN JOURNAL OF PHARMACOLOGY
·
2022
·
IF 5.195
Ningbo Cai,Bingtian Xu,Xing Li,Yunyun Qin,Mengfan Li,Kechun Chen,Jiangping Xu,Haitao Wang
The loss of tight junction (TJ) and adherens junction (AJ) proteins leads to the damage of the blood-brain barrier (BBB) during cerebral ischemia . Inhibition of cyclic nucleotide phosphodiesterase 4 (PDE4) by roflumilast (Roflu) protects against ischemic stroke-induced neuronal damage. However, the effects of Roflu on vascular endothelial injury and BBB integrity remain unknown. Here, we investigated whether and how Roflu protects against cerebrovascular endothelial injury caused by cerebral ischemia/reperfusion. We demonstrated that PDE4B knocking-down increased the expression of TJ and AJ proteins in human brain microvascular endothelial cells (HBMECs) subjected to oxygen-glucose deprivation reperfusion (OGD/R). Inhibition of PDE4 by Roflu (1.0 μM) showed similar effects as PDE4B knocking-down. We then found that Roflu activated Notch1/Hairy and enhancer of split 1 (Hes1) signaling. Consistently, the effects of Roflu on TJ and AJ proteins were reversed by the γ-secretase inhibitor DAPT or Hes1 knocking-down. Furthermore, Roflu (1.0 mg/kg) improved neurobehavioral outcomes and ameliorated BBB disruption in rats following ischemic stroke. Roflu also increased the levels of TJ proteins and AJ proteins in vivo . Collectively, these data suggest that Roflu is a promising compound for the prevention of BBB damage. The protective effects of Roflu are mediated through activation of the Notch1/Hes1 pathway.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2022
·Vol. 33
·期 8
·2620-2631
·
IF 3.348
Yuandou Wang,Shuxin Wang,Wenju Hu,Feng Su,Fusheng Liu,Suming Li
Amphiphilic block copolymers were synthesized by ring-opening polymerization of ε-caprolactone (ε-CL) using 4-arm poly(ethylene glycol) (4aPEG) as macroinitiator. 4aPEG and 4aPEG-PCL copolymers were end functionalized by introducing acrylic groups, and characterized using NMR, GPC, FT-IR and DSC analyses. Photo-crosslinked hydrogels were prepared by irradiation of acrylated polymer solutions under visible light (405 nm), using lithium phenyl-2,4,6-trimethylbenzoy-lphosphinate (LAP) as a photoinitiator. In situ gelation was achieved by subcutaneous injection of LAP containing copolymer solution into the abdomen of mice, followed by exposure to visible light. The hydrogels exhibit highly porous structure, high swelling up to 3300%, and excellent biocompatibility. The swelling ratio decreases with increase of poly(ɛ-caprolactone) (PCL) block length and polymer concentration of hydrogels. Doxorubicin hydrochloride (DOX·HCl) was loaded in hydrogels by soaking dried gel in a DOX solution. The drug loading efficiency is dependent on the swelling performance, reaching a maximum of 94.3%. In vitro drug release studies showed a burst release for the first 6 h, followed by a slower release up to 82% at 28 days. The release rate decreased with increase of hydrophobic block length or polymer concentration of hydrogels. Higher anti-cancer activity on A549 lung cancer cells was obtained for hydrogels with higher drug concentration, and shorter PCL block length. Therefore, 4aPEG-PCL hydrogels with outstanding biocompatibility, in situ gelation and prolonged drug release could be very promising for long-term local cancer therapy.
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Environmental Research and Public Health
·
2022
·Vol. 19
·期 5
·2934
·
IF 4.614
Hong Liu,Houwang Chen,Ning Ding
In this research, the preparation of a Ag3PO4/g-C3N4@MoS2photocatalyst and the performance and mechanism of degradation of 2-amino-4-acetaminoanisole (AMA) were studied. The phase composition and morphology of the synthesized samples were comprehensively characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), ultraviolet–visible diffuse reflectance (UV–Vis), and photoelectron spectroscopy (XPS). The catalytic performance of the photocatalyst was evaluated by the visible-light catalytic degradation of the AMA. The experimental results show that the Ag3PO4/g-C3N4@MoS2composite photocatalyst has stronger photocatalytic oxidation and reduction capabilities than Ag3PO4and Ag3PO4/g-C3N4. The material only decreases by 31.3% after five cycles of use, indicating that the material has good light stability. Free radical capture experiments prove that photo-generated holes (h+) and superoxide radicals (·O2−) are the main active substances in the photocatalytic process. The fundamental studies in the present research provide a new perspective for constructing an innovative type of visible-light photocatalyst and a new way to promote the photocatalytic degradation of organic pollutants.Keywords:catalysis;degradation;nanomaterials;Ag3PO4;heterojunction formation
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Yu-Shi Shen,Xiao-Hui Yao,Chen-Xi He,Run-Ze Hu,Jia-Xin Yang,Dong-Yang Zhang,Tao Chen
Modern bioreactors primarily focus on fixing enzymes on materials to form fixed bed reactors, but the reaction efficiency is unstable. More importantly, the enzymes easily fall off or even inactivate, and the fluid risks leaking due to excessive pressure during flow. In this study, a directional channel continuous microfluidic reactor with laccase covalently immobilized in the internal channel is made from natural wood. After dimethylacetamide/lithium chloride (DMAc/LiCl) treatment of delignified wood, the micropores (ray cells, pits and nanopores) in directional channel walls are fully exposed. When the fluid passes through, the turbulence near the pits increases, resulting in good mass and heat transfer, which enhances the catalytic efficiency of laccase . The reactor has good stability and maintains more than 80% substrate conversion in the pH range of 3–7 and the temperature range of 15–55 ℃. In the degradation of 4-nitrophenol (4-NP), a 94.42% degradation rate is achieved in only 30 min, and 86.93% efficiency is maintained after 25 cycles of catalysis, indicating its excellent reusability. The directional reactor with porous inner walls is simple to prepare and easy to scale up, which provides excellent convenience for continuous industrial production and shows great commercial application potential.
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 10
·1023-1030
·
IF 3.532
Hanxing Yang,Zhongguang Ji,Yanbo Zeng,Jian Zhang,Lifen Chen,Hailong Wang,Yiwen Yang,Longhua Guo,Lei Li
An aggregation-induced emission monomer-based fluorescent molecularly imprinted poly(ionic liquid) (AIE-FMIPIL) was synthesized for the first time with an AIE probe 4-(1,2,2-triphenylvinyl)phenyl acrylate (TPE), and an ionic liquid as dual functional monomers, and an ionic liquid as cross-linker. AIE-FMIPIL displayed a sphere-like shape and its average diameter was 410 nm. The absolute quantum yields of TPE and AIE-FMIPIL were 9.23% and 12.61%, respectively. The synergetic effect of TPE in the AIE-FMIPIL framework contributed to the higher quantum yield of AIE-FMIPIL. 4-Nitrophenol (4-NP) efficiently quenched AIE-FMIPIL with high fluorescence based on the Förster resonance energy transfer mechanism. The synthesized AIE-FMIPIL sensor was highly sensitive for 4-NP detection (linear range, 0.02–1.5 μM) in the optimal detection condition, with a low detection limit of 10 nM (S/N = 3). AIE-FMIPIL showed increased sensitivity and quenching efficiency compared with AIE-FMIP comprising a traditional monomer and cross-linker. AIE-FMIPIL exhibited selective binding to 4-NP because of the imprinted sites. AIE-FMIPIL was adopted to detect 4-NP in environmental samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Wenjing Gao,Bin Li,Ling Ling,Li Zhang,Shaoning Yu
Differentiating methicillin-sensitive and methicillin-resistant Staphylococcus aureus (MRSA and MSSA) is crucial for clinical diagnosis and anti-microbial treatment. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is an efficient tool for identifying pathogenic microorganisms at the bacterial species level. Here, we found that MRSA and MSSA can be differentiated by MALDI-TOF MS by employing (E)-propylα-cyano-4-hydroxyl cinnamylate (CHCA-C3) as the matrix, which shows great performance for proteins/peptides, especially hydrophobic proteins. The results show that the mass spectra profile of standard MRSA (ATCC 43300) is significantly different from the profiles of standard MSSA strains (ATCC 25923 and 29213) when using CHCA-C3 as the matrix compared to traditional matrix. The mass profiles had great reproducibility and were scarcely influenced by the growth medium. Due to the enhanced discrimination ability of CHCA-C3, we collected the mass spectra of 62 clinical S. aureus strains and selected four representative peaks for principal component analysis, which showed great differentiation. Our results suggest that employing a suitable matrix could enhance the discrimination ability of antibiotic-resistant bacteria by MALDI-TOF MS.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Yuenan Pan,Qi Chu,Shuang Guo,Sila Jin,Eungyeong Park,Yeonju Park,Yantao Sun,Lei Chen,Young Mee Jung
The purpose of this paper is to study the charge transfer (CT) process of a Ag/MBA/PAN system with different thicknesses of PAN and explore its SERS enhancement mechanism. It is found that as the PAN thickness is increased, the frequency and intensity of the characteristic MBA band gradually increases. The intensity of the b 2 mode increases significantly, which is attributed to the CT mechanism. As the PAN thickness is increased, the degree of CT (ρ CT ) gradually increases, which is due to the excited SPR from Ag. The electromagnetic enhancement mechanism, which occurs at the interface between PAN and Ag, generates hot electrons and rearranges the interface between PAN and Ag, thereby promoting electron transfer for the whole CT in Ag/MBA/PAN. These results show that CT occurs from the Fermi level of Ag to the LUMO level of MBA and then to the LUMO level of PAN. Additionally, the direction of CT is verified by 2D-COS. Therefore, this study not only reveals the ultrahigh SERS activity of the substrate but also explains the detailed process of regulating the substrate through the PAN thickness, which affects the SERS mechanism. This conductive polymer will open up a new horizon of SERS research and applications.
✓ 已验证
产品关联
DOI
高影响因子
Green Energy & Environment
·
2022
·
IF 12.781
Yang Zhou,Qiang Zeng,Hongyan He,Kejia Wu,Fuqiao Liu,Xuehui Li
In order to better understand the specific substituent effects on the electrochemical oxidation process of β -O-4 bond, a series of methoxyphenyl type β -O-4 dimer model compounds with different localized methoxyl groups, including 2-(2-methoxyphenoxy)-1-phenylethanone, 2-(2-methoxyphenoxy)-1-phenylethanol, 2-(2-methoxyphenoxy)-1-(4-methoxyphenyl)ethanone, 2-(2-methoxyphenoxy)-1-(4-methoxyphenyl)ethanol, 2-(2,6-dimethoxyphenoxy)-1-(4-methoxyphenyl)ethanone, 2-(2,6-dimethoxyphenoxy)-1-(4-methoxyphenyl)ethanol have been selected and their electrochemical properties have been studied experimentally by cyclic voltammetry, and FT-IR spectroelectrochemistry. Combining with electrolysis products distribution analysis and density functional theory calculations, oxidation mechanisms of all six model dimers have been explored. In particular, a total effect from substituents of both para -methoxy (on the aryl ring closing to C α ) and C α -OH on the oxidation mechanisms has been clearly observed, showing a significant selectivity on the C α -C β bond cleavage induced by electrochemical oxidations.
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Xiaoxiao Li,Yu Wang,Jing Wu,Zhengyu Jin,Lubbert Dijkhuizen,Maher Abou Hachem,Yuxiang Bai
Solid-liquid thermo-reversibility, lacking in raw starch is an important property for gels allowing repeated thermal processing in food . In this work, the Thermoproteus uzoniensis 4-α-glucanotransferase (TuαGT) was used to modify five different starches from potato, corn , rice, wheat, and pea and the thermo-reversibility of the modified starch gels was evaluated by rheological assay. Only modified potato starch (MPS) exhibited thermo-reversibility, which prompted us to perform detailed analyses of the structural and gel properties of this product. The enzymatic modification resulted in a marked decrease in amylopectin side chains with a degree of polymerization (DP) < 23, while the contents of side chains with DP 23–43 increased. These data indicated that TuαGT preferentially extended the outer chains of amylopectin using amylose as a donor, which resulted in 17% increase in the amylopectin molecular weight to 3.0 × 10 7 g/mol. Notably, MPS displayed a delayed retrogradation and improved stability in four freeze-thaw cycles (−70 °C and 25 °C) as compared to the native starch. These analyses supported a model to explain the TuαGT catalyzed conversion of potato starch to a thermo-reversible product. Importantly, the MPS thermo-reversible 6% (w/v) gel showed comparable rheological properties to a 1.5% (w/v) κ-carrageenan hydrocolloid , highlighting the potential of MPS as a substitute to κ-carrageenan and other hydrocolloids of emerging health concerns. This work offers a starch based thermo-reversible gel and a methodological framework to broadens the use of starch into novel application as a food ingredient.
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS SCIENCE IN SEMICONDUCTOR PROCESSING
·
2021
·
IF 3.927
Qiulian Li,Shizhong Sun,Xinyu Li,Xiang Li,Xiu Liu,Daoyong Zhang,Na Yu,Shurong Wang
Kesterite Cu 2 ZnSn(S,Se) 4 (CZTSSe) is emerging as one of the most promising materials owing to its low-cost, non-toxicity, similar electronic properties with Cu 2 (In,Ga)Se 2 (CIGS). However, the current highest power conversion efficiency (PCE) for CZTSSe device is far below the CIGS because of the high open-circuit voltage (V oc ) deficit and low fill factor (FF). Here, we fabricated CZTSSe thin film from 2-methoxyethanol solutions and investigated the effects of different Sn (Sn 2+ vs Sn 4+ ) oxidation states of precursor solution and selenization temperature on the crystal quality of the CZTSSe thin film and device performances. By characterizations and analyses, we found that incomplete redox reaction caused by the off-stoichiometric component in Sn 2+ precursor solution led to the formation of detrimental secondary phases such as SnSe, Cu 2 Se, and SnSe 2 in the CZTSSe thin film. The existence of volatile SnSe could cause the formation of voids and thus affected the FF and V oc . The reaction from Sn 4+ precursor solution to absorber material avoided the formation of SnSe. Ultimately, the Sn 4+ thin film showed better features in terms of improving crystal quality and reducing V oc deficit that limited the efficiency of kesterite CZTSSe thin film solar cells. Besides, we demonstrated that an appropriate selenization temperature could further reduce the number of secondary phases and improve the surface morphology and crystallinity of the CZTSSe thin film. As a result, we obtained CZTSSe thin film solar cells with 8.27% PCE from the Sn 4+ precursor solution under the selenization temperature of 580 °C.
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Xiaoyan Sun,Yuxin Guo,Ruizhi Wen,Hongchang Li
Cu 2+ is a major environmental pollutant. An efficient measurement for Cu 2+ is urgently needed. In this report, we have developed a new sensitive and selective ratiometric sensing platform using 7-amino-4-methylcoumarin (AMC) for detecting Cu 2+ in real samples. In the presence of Cu 2+ , o-phenylenediamine (OPD) could be catalytically oxidized to 2,3-diaminophenazine (DAP), which could react with AMC, leading to quench the fluorescence intensity of AMC at 438 nm. Meanwhile, DAP provided a new emission peak at 557 nm. Based on the efficient overlapped spectrum of AMC and DAP, a ratiometric sensing platform through fluorescence resonance energy transfer (FRET) was carried out. Furthermore, the as-proposed strategy displayed the linear relationship in the wide range from 6 to 250 μM with a low detection limit of 0.059 μM, and the recoveries of the spiked samples in real samples ranged from 86.5% to 110.1%. Moreover, comparing the visual fluorescence colors of the real samples with the standard colorimetric card, we used the as-proposed strategy as a solid-based platform for realizing an efficient semi-quantitative detection of Cu 2+ via naked-eye visual fluorescence mode without any complicated instrument and operation. The above results implied that the as-proposed strategy could be used in the practice determination of Cu 2+ .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Jingyi Zhu,Yixin Zhu,Wenjun Zhou
Oxygen vacancies (OVs) were introduced into Ni-based layered double hydroxides (LDHs) through Cu doping, and the catalytic performance of the resulting Ni x Cu-LDHs were investigated for peroxymonosulfate (PMS) activation and methyl 4-hydroxybenzoate (MeP) degradation. Compared with that of Ni-LDH, the catalytic performance of Ni x Cu-LDHs were significantly enhanced and increased with increasing OV content in the catalysts, indicating that Cu doping introduced OVs into Ni x Cu-LDHs and greatly improved their catalytic activity with PMS. Quenching experiments and EPR analyses confirmed that oxidation processes dominated by superoxide radicals (O 2 •− ) and singlet oxygen ( 1 O 2 ), rather than sulfate radicals (SO 4 •− ) or hydroxyl radicals (•OH) used by traditional LDH catalysts, were responsible for MeP degradation by Ni 15 Cu-LDHs. In addition, quenching experiments with different systems showed the fate of reduced SO 4 •− and •OH, and demonstrated that O 2 •− and 1 O 2 concentrations grew with increasing OV content, confirming that the presence of OVs affected the process of PMS activation. Notably, O 2 •− mainly originated from adsorbed oxygen or dissolved oxygen (DO) by acquiring electrons from OVs in Ni 15 Cu-LDHs, since OVs possess abundant localized electrons. Consequently, an OV-mediated oxidative mechanism was proposed for Ni 15 Cu-LDHs/PMS. This study provides new clues for enhancing the catalytic performance of LDH catalysts by introducing OVs via metal doping in PMS-based AOPs systems.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）