Scientific Reports
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2024
·Vol. 14
·期 1
·1-10
·
IF 3.8
Guo Jingqiao,Xin Jianhui,Wang Jine,Li Zhen,Yang Jianlei,Yu Xue,Yan Mengxia,Mo Jiangyang
Tetracyclines (TCs) rank second globally in the use of animal infection therapy and animal husbandry as growth promoters among all antibiotics. However, large amounts of TCs residue in food products and more than 75% of TCs are excreted into the environment, causing adverse effects on the ecological system and human health. It has been challenging to simultaneously realize low-cost, rapid, and highly selective detection of TCs. Here, inspired by the fluorogenic reactions between resorcinol and catecholamines, we find the fluorescence quenching ability of tetracycline (TC) and firstly propose a fluorescent “turn-off” detection of TC using dopamine and 4-fluororesorcinol. The optimal reaction condition for the fluorescent assay is investigated and the optimized probe showed a good limit of detection (LOD of 1.7 µM) and a wide linear range (10 µM to 350 µM). Moreover, this fluorescent assay proved to be an effective tool for detecting TC in river, Sprite, and beer samples, which represent the aquatic environments and food and may contain tetracyclines residues. Finally, the high selectivity of the method for TC has been confirmed by eliminating the interference from common substances. The proposed strategy provides a high-efficiency and selective solution for the detection of TCs in environment and food and the application fields of this fluorescent assay could be further expanded in the future.
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JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
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2017
·Vol. 94
·期 9
·1153-1164
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IF 1.421
Yang Shu,Li Guode,Saleh Ahmed S. M.,Yang Hongli,Wang Na,Wang Peng,Yue Xiqing,Xiao Zhigang
β-Sitosterol (Sit) and stearic acid (SA) were combined at varying ratios (w/w) and added to sunflower oil (SFO) at the concentration of 20 g/100 g oil for preparing edible fat-like oleogel. The oleogel was characterized using an optical microscope, Fourier transform infrared spectroscopy (FTIR), X-ray diffractometer (XRD), differential scanning calorimeter, and texture analyzer. The oil-binding capacity, melting temperature, and firmness of the oleogel increased with the increase in the amount of SA in the gelator combination (Sit:SA, w/w). The microscopic analysis showed that the gel network formed based on the crystallization and self-organization of gelator molecules, and both gelators showed an independent crystalline behavior in the oleogel. In addition, the FTIR spectra showed that the gel network formed via physical entanglements and was stabilized by non-covalent interactions such as hydrogen bonding. Furthermore, XRD diffraction patterns indicated high lateral packing of molecular layers in oleogel prepared with the Sit and SA combination compared with oleogel prepared with a single gelator. On the other hand, for studying the effect of varying concentrations of gelator combinations, the Sit3:SA2 (w/w) combination was added to SFO at concentrations of 10, 15, 20, 25, and 30 g/100 g oil. Specific characteristics such as the oil-binding capacity and firmness of the oleogel improved as the concentration of the gelator combination (Sit3:SA2) increased from 10 up to 30 g/100 g oil. Therefore, it can be concluded that the saturated fat alternative oleogel can be prepared from SFO with a specific Sit and SA combination ratio and concentration.
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FIBERS AND POLYMERS
·
2017
·Vol. 18
·期 7
·1250-1254
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IF 1.113
Chen Yingbo,Liu Huiqiang,Hu Xiaoyu,Cheng Bowen,Liu Dongqing,Zhang Yufeng,Nair Sankar
Membranes with high water permeation capability as well as high rejection to dye molecules are very important for dye separation. In this study, a metal organic framework structure (Cu-BTC) was fabricated in situ on a poly(vinyl difluoride) hollow fiber support for nanofiltration of dye solution. In order to protect the Cu-BTC layer, the surface was coated by crosslinking polyvinyl alcohol. The composite membranes showed no rejection to divalent salts and high rejection to Congo Red dye. It was noticed that several Cu-BTC layers could enhance dye rejection of the composite membranes. This novel composite membrane showed promising applications in the separation of dye molecules from aqueous solutions containing dissolved salts.
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COLLOID AND POLYMER SCIENCE
·
2017
·Vol. 295
·期 10
·1887-1895
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IF 1.723
Zhu Zhou,Kang Wanli,Yang Hongbin,Wang Pengxiang,Zhang Xiangfeng,Yin Xia,Lashari Zeeshan Ali
A method based on host-guest inclusion system was used to investigate the salt thickening mechanism of poly [acrylamide-co-N, N′-dimethyl (methylmethacryloyl ethyl) ammonium propane sulfonate-co-cetyl dimethyl allyl ammonium chloride] (PADC). The salt thickening behavior of PADC solution was studied by rotational viscometer, and its average hydrodynamic diameter ( D
h ) was characterized by dynamic light scattering (DLS). The formed host (β-cyclodextrin)-guest (hydrophobic groups of PADC) inclusion system was confirmed by scanning electron microscopy (SEM). A method of characterizing the hydrophobic association strength and determining the hydrophobic association contribution rate (HACR) was introduced by the inclusion of β-cyclodextrin (β-CD). The results showed that the apparent viscosity and the D
h of PADC solution increased with the increase of salt concentration. In addition, the types of salt also affected the thickening property of PADC solution. Moreover, the results of HACR indicated that the hydrophobic association strength of PADC solution was enhanced in the presence of salts, which was confirmed by fluorescent method. Thus, it could be concluded that the salt thickening of PADC solution was caused by the destruction of ionic bonds in betaine monomer and the enhancement of hydrophobic association strength.
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MICROCHIMICA ACTA
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2017
·Vol. 184
·期 8
·2959-2964
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IF 4.58
Huang Wei,Liang Yun,Deng Yuequan,Cai Yanhua,He Yi
The authors report on a method for visual and spectrophotometric determination of silver (I) by using Prussian Blue nanoparticles (PB NPs) as a colorimetric probe. It is based on the finding that the blue PB NPs are converted into colorless silver ferrocyanide ions in the presence of Ag(I) due to the establishment of a dissolution-precipitation equilibrium. The change in absorbance at 700 nm is related to the concentration of Ag(I) in the range from 0.01 to 3 μM. The visual detection limit is 3 μM. The assay has a good selectivity for Ag(I) over other common ions. It was successfully applied to the determination of Ag(I) in spiked samples of river and lake waters.
Schematic presentation of a visual and spectrophotometric method for determination of silver(I) by using Prussian Blue nanoparticles (PB NPs) as optical probes.
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Food Analytical Methods
·
2017
·Vol. 11
·期 3
·797-807
·
IF 2.038
Yin Qinhong,Zhu Yanqin,Yang Yaling
In this study, a two-step extraction technique was developed for extraction and preconcentration of parabens from beverage samples using ionic liquid dispersive liquid–liquid microextraction (IL-DLLME) and magnetic solid-phase extraction (MSPE). In this IL-DLLME followed by MSPE method, ionic liquid (IL, 1-octyl-3-methylimidazolium hexafluorophosphate) formed hydrophobic microdroplets in beverage samples as an extractant of parabens; after the IL-DLLME process was completed, graphene modified Fe 3 O 4 nanoparticles (Fe 3 O 4 @G) were placed to adsorb and isolate IL from the sample solution. After the supernatant was carefully moved, acetonitrile was added to elute the IL containing parabens from Fe 3 O 4 @G. The experimental variables affecting the extraction procedure have been systematically studied. Under optimal conditions, the detection limits were less than 1.53 ng/mL and the linear detection ranges were 2–500 ng/mL ( R
2 ≥ 0.998) for these analytes. The recoveries for spiked samples were 58.8–89.2% and satisfactory precision (RSD ≤ 4.8%) were obtained.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2017
·Vol. 33
·期 3
·507-512
·
IF 1.024
Guo Zhuo,Luo Xianke,Li Yahui,Li Dongdi,Zhao Qinai,Li Mengmeng,Ma Chi,Zhao Yanting
An electrochemical method for simultaneous determination of ascorbic acid(AA), dopamine(DA) and uric acid(UA) was developed based on RGO-Ag/PANI modified glassy carbon electrode(RGO-Ag/PANI/GCE, RGO= reduced graphene oxide, PANI=polyaniline). Owing to the synergetic effect among RGO, Ag and PANI, the RGO-Ag/PANI based sensor not only improved the electrochemically catalytic oxidation of AA, DA and UA, but also resolved the overlapping anodic peaks. Under the optimized conditions, the calibration curves were obtained in the concentration ranges of 50—1000, 5—200 and 20—350 μmol/L, and the detection limits(S/N=3) of 0.5, 0.2 and 0.2 μmol/L were obtained for AA, DA, and UA, respectively, in neutral phosphate buffer solutions(PBS). The RGO-Ag/PANI/GCE sensor was successfully applied to the determination of AA, DA, and UA in bovine serum samples and showed high selectivity, sensitivity, and reproducibility.
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Stem Cell Research & Therapy
·
2017
·Vol. 8
·期 1
·1-10
·
IF 4.211
Li Lin,Chu Lisheng,Fang Yan,Yang Yan,Qu Tiebing,Zhang Jianping,Yin Yuanjun,Gu Jingjing
Background Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) is one of the new therapeutic strategies for treating ischemic stroke. However, the relatively poor migratory capacity of BMSCs toward infarcted regions limited the therapeutic potential of this approach. Pharmacological preconditioning can increase the expression of CXC chemokine receptor 4 (CXCR4) in BMSCs and enhance cell migration toward the injury site. In the present study, we investigated whether tetramethylpyrazine (TMP) preconditioning could enhance BMSCs migration to the ischemic brain and improve functional recovery through upregulating CXCR4 expression.Methods BMSCs were identified by flow cytometry analysis. BMSCs migration was evaluated in vitro by transwell migration assay, and CXCR4 expression was measured by quantitative reverse transcription-polymerase chain reaction and western blot analysis. In rats with focal cerebral ischemia, the neurological function was evaluated by the modified neurological severity score, the adhesive removal test and the corner test. The homing BMSCs and angiogenesis were detected by immunofluorescence, and expression of stromal cell-derived factor-1 (SDF-1) and CXCR4 was measured by western blot analysis.Results Flow cytometry analysis demonstrated that BMSCs expressed CD29 and CD90, but not CD34 and CD45. TMP pretreatment dose-dependently induced BMSCs migration and CXCR4 expression in vitro, which was significantly inhibited by AMD3100, a CXCR4 antagonist. In rat stroke models, we found more TMP-preconditioned BMSCs homing toward the infarcted regions than nonpreconditioned cells, leading to improved neurological performance and enhanced angiogenesis. Moreover, TMP-preconditioned BMSCs significantly upregulated the protein expression of SDF-1 and CXCR4 in the ischemic boundary regions. These beneficial effects of TMP preconditioning were blocked by AMD3100.Conclusion TMP preconditioning enhances the migration and homing ability of BMSCs, increases CXCR4 expression, promotes angiogenesis, and improves neurological performance. Therefore, TMP preconditioning may be an effective strategy to improve the therapeutic potency of BMSCs for ischemic stroke due to enhanced BMSCs migration to ischemic regions.
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Microsystems & Nanoengineering
·
2024
·Vol. 10
·期 1
·1-9
·
IF 7.3
Li Fuwang,Wei Jian,Qin Xiaomei,Chen Xue,Chen Dawei,Zhang Wentao,Han Jiaguang,Yuan Libo,Deng Hongchang
Field-controlled micromanipulation represents a pivotal technique for handling microparticles, yet conventional methods often risk physical damage to targets. Here, we discovered a completely new mechanism for true noncontact manipulation through photothermal effects, called thermal-optical tweezers. We employ a laser self-assembly photothermal waveguide (PTW) for dynamic microparticle manipulation. This waveguide demonstrates superior photothermal conversion and precision control, generating a nonisothermal temperature field. The interaction of thermal convection and thermophoresis within this field creates a microfluidic potential well, enabling noncontact and nondestructive particle manipulation. By varying the path of PTWs in lithography and manipulating laser loading modes, diverse manipulation strategies, such as Z-shaped migration, periodic oscillation, and directional transport, are achievable. Our innovative noninvasive micromanipulation technology minimizes not only physical damage to target objects but also enables precise and diverse manipulation of micro entities, opening up new avenues for the photothermal control of cells and biomolecules.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-19
·
IF 10.6
Ye Yan,Zhong Wenzhen,Luo Ruifeng,Wen Hongzhi,Ma Ziyang,Qi Shanshan,Han Xiaoqin,Nie Wenbiao,Chang Degui,Xu Runchun,Ye Naijing,Gao Fei,Zhang Peihai
Background The complex etiology and pathogenesis underlying Chronic Non-Bacterial Prostatitis (CNP), coupled with the existence of a Blood Prostate Barrier (BPB), contribute to a lack of specificity and poor penetration of most drugs. Emodin (EMO), a potential natural compound for CNP treatment, exhibits commendable anti-inflammatory, anti-oxidant, and anti-fibrosis properties but suffers from the same problems as other drugs.Methods By exploiting the recognition properties of lactoferrin (LF) receptors that target intestinal epithelial cells (NCM-460) and prostate epithelial cells (RWPE-1), a pathway is established for the transrectal absorption of EMO to effectively reach the prostate. Additionally, hyaluronic acid (HA) is employed, recognizing CD44 receptors which target macrophages within the inflamed prostate. This interaction facilitates the intraprostatic delivery of EMO, leading to its pronounced anti-inflammatory effects. A thermosensitive hydrogel (CS-Gel) prepared from chitosan (CS) and β-glycerophosphate disodium salt (β-GP) was used for rectal drug delivery with strong adhesion to achieve effective drug retention and sustained slow release. Thus, we developed a triple-targeted nanoparticle (NPs)/thermosensitive hydrogel (Gel) rectal drug delivery system. In this process, LF, with its positive charge, was utilized to load EMO through dialysis, producing LF@EMO-NPs. Subsequently, HA was employed to encapsulate EMO-loaded LF nanoparticles via electrostatic adsorption, yielding HA/LF@EMO-NPs. Finally, HA/LF@EMO-NPs lyophilized powder was added to CS-Gel (HA/LF@EMO-NPs Gel).Results Cellular assays indicated that NCM-460 and RWPE-1 cells showed high uptake of both LF@EMO-NPs and HA/LF@EMO-NPs, while Raw 264.7 cells exhibited substantial uptake of HA/LF@EMO-NPs. For LPS-induced Raw 264.7 cells, HA/LF@EMO-NPs can reduce the inflammatory responses by modulating TLR4/NF-κB signaling pathways. Tissue imaging corroborated the capacity of HA/LF-modified formulations to breach the BPB, accumulating within the gland's lumen. Animal experiments showed that rectal administration of HA/LF@EMO-NPs Gel significantly reduced inflammatory cytokine expression, oxidative stress levels and fibrosis in the CNP rats, in addition to exerting anti-inflammatory effects by inhibiting the NF-κB signaling pathway without obvious toxicity.Conclusion This triple-targeted NPs/Gel rectal delivery system with slow-release anti-inflammatory, anti-oxidant, and anti-fibrosis properties shows great potential for the effective treatment of CNP.Graphical Abstract
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Science China-Materials
·
2024
·
IF 6.8
Gong Jiaqi,Ji Shilei,Li Jintian,Wei Hudie,Mao Weiwei,Hu Jing,Huang Wen,He Xuemin,Li Xing’ao,Chu Liang
Memristors have great potential in neural network computation. Perovskite memristors exhibit excellent resistive-switching (RS) properties between high resistance state (HRS) and low resistance state (LRS) state under applied voltage due to the extraordinary ion migration and superior charge transfer. However, the stability issue of traditional three-dimensional (3D) perovskites is still challenging. Here, one-dimensional (1D) (CH 3 ) 3 SPbI 3 perovskite passivation layer was in-situ formed on 3D perovskite film, which was further applied in stable synaptic memristor. The memristor was provided with three resistance states due to the heterojunction electric field coupled with ion migration. The on/off ratio of memristors was obviously improved from 10 to over 60. The RS characteristics of 3D/1D perovskite memristor remained unchanged after 10 3 s read and 300 switching cycles. The 3D/1D perovskite memristor effectively exhibited versatile synaptic plasticity behaviors including long-term potentiation, long-term depression and paired-pulse facilitation by controlling the input voltages. Notably, the novel device provides a new candidate for next-generation neuromorphic computing.
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CABI Agriculture & Bioscience
·
2024
·Vol. 5
·期 1
·1-9
·
IF 2.8
Kou Jing,Zhu Zheng-Yang,Wang Su,Zhang Yu,Wang Jie,Jaworski Coline C.,Harwood James D.,Jing Lan,Desneux Nicolas,Di Ning
The heavy metal cadmium (Cd) leads to significant bottom-up effects on food chains of plants, herbivores, and predators in agroecosystems. Through the transfer and accumulation of Cd. In addition to the indirect effects of this cascading effects, predators Orius sauteri fed with artificial diets containing Cd also have a direct effect. We found that O. sauteri fed with sucrose solutions containing Cd at high concentrations of 125 mg/L and 625 mg/L significantly decreased the survival of the 3rd and 5th instar, female, and male adults (except 125 mg/L) of O . sauteri . However, the survival of O. sauteri nymphs was significantly increased when they fed with low concentrations of 1 mg/L, 5 mg/L and 25 mg/L Cd sucrose solutions. Cd stress has differing effects on the LC 50 of each age of O. sauteri , the lowest LC 50 for male adults (153 mg/L), followed by 3rd instar nymphs (192 mg/L), 5th instar nymphs (289 mg/L) and female adults (383 mg/L). It is interesting that 1 mg/L, 5 mg/L, and 25 mg/L of sucrose solutions containing Cd significantly improved the predation capacity of the nymphs of O. sauteri to the B emisia tabaci pupa. The predation ability of the male adults of O . sauteri on B . tabaci pupa were significantly improved, but significantly decreased in the female adults when they fed with 125 mg/L and 625 mg/L sucrose solutions containing Cd. This difference needs to be studied in depth in the future, controlling Cd contamination and protecting natural enemies to enhance the biological control of pests. Graphical Abstract
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危险品化学品经营许可证（带存储）