Pharmaceutics
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2026
·Vol. 18
·期 2
·253
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IF 6.9
Xiwen Zeng,Sizhuo Liu,Qianhui He,Yanwen Ling,Jingqi Sun,Yang Ping,Jin Su
Background: Liver cancer is a complex malignant tumor; gambogic acid (GA) has significant anti-cancer potential, but poor water solubility and low bioavailability limit its clinical application. In this paper, by integrating nanocrystal (NC) technology and an active targeting strategy, a new nanoagent—folic acid-modified phospholipid–gambogic acid nanocrystals (GA-NCs@FA)—was developed to improve the delivery efficiency and therapeutic effect of GA in the treatment of liver cancer.Methods: GA-NCs@FA was prepared by the CO2-assisted precipitation method and the thin-film hydration method. The in vitro anti-tumor activity of GA-NCs@FA was evaluated by cytotoxicity, as well as a scratch and uptake test. A HepG2 tumor-bearing nude mouse model was established to investigate the in vivo distribution and tumor targeting of GA. The in vivo anti-tumor activity was evaluated by the tumor inhibition rate, and the pathological changes of organs in each group were observed by H&E staining.Results: GA-NCs@FA significantly reduced HepG2 cell viability (IC50: 0.50 μg·mL−1) and migration ability (48 h healing rate: 11.50%) and enhanced intracellular fluorescence intensity. In vivo analysis showed that GA-NCs@FA significantly increased the accumulation of drugs in tumor tissues by active targeting and achieved a tumor growth inhibition rate of 70.9%. Histopathology confirmed that GA-NCs@FA induced the most obvious nuclear pyknosis and necrosis in tumor tissues while maintaining good biosafety.Conclusions: GA-NCs@FA significantly prolongs the systemic circulation time of the drug and enhances intratumoral accumulation; therefore, it is a method that can be considered for active targeting and treatment of liver cancer.
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ACS Biomaterials Science & Engineering
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2026
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IF 6
Jie Bai,Ying Bao,Shan Li,Zicheng Guo,Jie Xu,Rong Huang,Jing Yang,Cunqin Lv,Jianhong Liu,Dinglin Zhang
Reactive oxygen species (ROS) exert critical influence in inflammatory bowel disease (IBD). Metal–polyphenol nanoparticles (MPNs) have exhibited a promising ability to regulate ROS levels and restore homeostasis in the context of IBD. However, the relationship between therapeutic efficacy and the type of MPNs requires further investigation. In this research, we synthesized tailorable MPNs by using epigallocatechin gallate (EGCG) and Mg2+, Zn2+, Ca2+, and Cu2+ and investigated their therapeutic efficacy for IBD. In vitro experiments confirmed that these MPNs exhibited pH and ROS responsiveness, increased the stability of EGCG, and improved ROS elimination. In vivo experiments demonstrated that these MPNs can effectively accumulate at sites of colonic inflammation after intraperitoneal injection and can continuously scavenge ROS, downregulate inflammatory factors, and restore the intestinal barrier integrity. Compared with other MPNs, Zn-EGCG nanoparticles (NPs) significantly alleviate symptoms of DSS-induced acute colitis in mice. Our work provides strong evidence to support the rational design of MPNs to regulate inflammation.
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BIOCHEMICAL PHARMACOLOGY
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2026
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IF 6.5
Man Gao,Xue Yang,Xiuxia Wang,Tong Liu
Preeclampsia (PE), a pregnancy-specific disorder characterized by placental dysfunction and severe maternal-fetal complications, involves ferroptosis—an iron-dependent cell death driven by oxidative stress and lipid peroxidation. This study investigated the role of zinc-finger protein 36 (ZFP36) in regulating ferroptosis in trophoblast cells and its underlying mechanisms. In vitro , hypoxia/reoxygenation was applied to human trophoblast HTR-8/SVneo cells to model ischemic injury. ZFP36 was found to inhibit oxidative stress and ferroptosis by suppressing nuclear receptor coactivator 4 (NCOA4)-mediated ferritinophagy. Mechanistically, ZFP36 binds to the 3′ untranslated regions (3′UTRs) of NCOA4 mRNA, promoting its degradation and thereby attenuating ferritinophagy. In vivo , lentiviral overexpression of ZFP36 in a rat model of PE (induced by reduced uteroplacental perfusion pressure) alleviated clinical symptoms and reduced NCOA4-mediated ferroptosis. Collectively, these results demonstrate that ZFP36 regulates ferroptosis by modulating NCOA4-dependent ferritinophagy, offering a potential therapeutic strategy for PE through targeting ferroptosis.
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JOURNAL OF NANOBIOTECHNOLOGY
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2026
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IF 15
Hu Jingde,Mei Shaoxun,Song Qixiang,Wang Xinru,Yang Shuaiqi,Wu Guohuo,Wang Zhen,Zhang Xiangchun,Ji Yuntao,Jiang Xiasen,Qu Changqing
Bacterial resistance, recurrent infections, and impaired wound healing pose significant challenges in clinical wound management. Natural biomass-derived carbon dots (CDs) have attracted considerable attention due to their synergistic antibacterial, anti-inflammatory, and biosafety, which facilitate the healing of infected wounds. In this study, carbon dots derived from Toona sinensis gum polysaccharide (TGP-CDs) were integrated into a gelatin-chitosan hydrogel to construct TGP-CDs@Gel. Experimental results demonstrated that TGP-CDs@Gel exhibits strong light-activated ROS generation capability, effectively disrupting bacterial biofilms and compromising membrane integrity through photodynamic antibacterial mechanisms. The composite also significantly promoted L929 fibroblast migration and protected RAW264.7 macrophages and N2 nematodes from oxidative stress. Importantly, TGP-CDs@Gel exhibited potent photodynamic antibacterial activity, as well as the regulation of pro-inflammatory cytokines and angiogenesis-related proteins (i.e., CD31, VEGF) in MRSA-infected wound, thereby significantly accelerating wound healing. Moreover, comprehensive biosafety evaluation confirmed its excellent biocompatibility across multiple models, including in vitro cell cultures, zebrafish, and nematodes. Collectively, TGP-CDs@Gel demonstrates promising photodynamic antibacterial and anti-inflammatory functions, providing a novel strategy for tissue regeneration and extending the application of Toona sinensis gum in biomaterials. Graphical abstract
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2026
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IF 5.2
Chong Li,Yi Zhang,Guixiang Yang,Qian Jin,Wang Chen,Ruihua Zhang,Tong Shi,Jingchen Wei,Xuejun Chen,Liqin Li
The rapid aging kinetics of soman (< 2 min) necessitate the development of effective prophylactic countermeasures, as conventional post-exposure therapies are often inadequate. To address this challenge, we engineered a novel separable microneedle (MN) system based on silk fibroin (SF) for sustained transdermal delivery of huperzine A (Hup A)—a reversible acetylcholinesterase inhibitor with demonstrated blood - brain barrier penetration capabilities. The optimized SFMNs design featured a distinctive tip-separation mechanism that facilitated reliable detachment of drug-loaded needle tips within 2 min after dermal application. The characterization results showed that the MN patch exhibited robust mechanical strength (155.49±11.18 N/patch), uniform morphology, effective skin penetration and homogeneous distribution of Hup A within the needle tips. Notably, the SFMNs platform provided controlled release kinetics, achieving sustained in vitro release of Hup A over 12 h, with an initial burst phase conducive to rapid drug onset. Pharmacokinetic evaluations in mice revealed that transdermal delivery via SFMNs (4 mg/kg Hup A) maintained therapeutic plasma concentrations for up to 12 h, significantly prolonging the mean residence time (11.87 ± 0.88 h) compared to oral administration (2.39 ± 0.31 h). This sustained-release profile markedly reduced systemic toxicity; whereas oral Hup A elicited severe cholinergic adverse effects (e.g., sweating, tremors), SFMNs-mediated delivery circumvented these toxic responses. In a prophylactic efficacy study, Hup A-loaded SFMNs (4 mg/kg) conferred complete protection (100% survival) for 12 h against a 95% lethal dose soman challenge, substantially extending the protective window compared to both oral Hup A (2 h) and intramuscular pyridostigmine bromide (45 min). These findings demonstrate that the SFMNs platform represents a promising, convenient, and well-tolerated strategy for long-acting prophylaxis against soman intoxication.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2026
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IF 7.1
Xinjie Wang,Jinzhou Lu,Dan Yu,Shiyin Tao,Yang Zhang,Jiayou Li,Jianxing Yu
This study aims to evaluate the inhibitory effect and molecular mechanism of orange peel (OP) and its fermented product (FOP) by Rhizopus stolonifer JP1118 on the accumulation of biogenic amines (BAs) in Chinese rice wine (Huangjiu). UHPLC-Q-TOF-MS/MS technology was utilized to analyze the differences in metabolites between OP and FOP. Huangjiu fermentation experiments were conducted to compare the effects of different OP/FOP concentrations on BAs formation, and molecular docking was used to verify the interaction between flavonoid components and BAs synthesis-related decarboxylases. The results indicated that the content of 13 flavonoid compounds in FOP was more than double that in OP. The 4 g/L FOP treatment group demonstrated the most inhibition of BAs formation, and the inhibition on histamine was particularly significant. Molecular docking results confirmed that 11 flavonoids in FOP could stably bind to histidine decarboxylase (HDC), and 10 flavonoids could stably bind to tyrosine decarboxylase (TDC). They occupy the active sites of the enzymes via hydrogen bonding and hydrophobic interactions, thereby impeding substrate binding and inhibiting the synthesis of histamine and tyramine. This research offers a technical solution for low-amine Huangjiu, references for BAs control in other fermented foods and agricultural by-product recycling, with academic and industrial significance.
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ANALYTICA CHIMICA ACTA
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2026
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IF 6.1
Qing Li,Yage Guan,Qian Wang,Daxiang Gui,Yingqiang Fu
s Background 2,4-Dichlorophenoxyacetic acid (2,4-D) is a widely used herbicide whose environmental residues pose threats to ecology and health. Copper ions (Cu 2+ ), as common heavy metal pollutants, can exhibit synergistic toxicity with 2,4-D, exacerbating environmental risks. Traditional single-emission fluorescent probes are typically limited to detecting a single target or operating in a single mode, making it challenging to achieve simultaneous monitoring of multiple pollutants. Therefore, developing novel multifunctional fluorescent probes capable of concurrently detecting both Cu 2+ and 2,4-D holds significant importance. Results This work successfully synthesized Rhodamine-modified carbon quantum dots (R-CQDs) via a one-step hydrothermal method. The material exhibits dual emission fluorescence peaks at 464 nm and 544 nm. Cu 2+ selectively quenches the 464 nm fluorescence, while 2,4-D restores fluorescence intensity by competing with Cu 2+ for coordination via its carboxyl group. The detection limits for Cu 2+ and 2,4-D are 2.71 × 10 −3 μM and 1.86 × 10 −3 μM, respectively. Further integration with a smartphone platform combined photocurrent sensing and fluorometric colorimetry, enabling expansion to visual, portable triple-mode (fluorescence, photocurrent, colorimetry) detection. Detection limits for Cu 2+ and 2,4-D reached 0.01382 μM and 0.01925 μM, respectively. This achieved highly sensitive and selective cascade detection of 2,4-D and Cu 2+ . Significance: This multi-signal complementary strategy significantly enhances interference resistance and reliability, providing a portable, low-cost, and universal solution for on-site and rapid monitoring of heavy metal and pesticide residues in environmental and food matrices.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2026
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IF 4.8
Pei Wu,Yang Jiao,Li Cheng,Cheng Yao,Xiong Chen
A novel near-infrared luminescent fluorescent composite material, TCPP-His@ZIF-8, was successfully constructed. Through an amide condensation reaction, Tetrakis (4-carboxyphenyl) porphyrin (TCPP) was covalently anchored onto the surface of L-histidine (His) -modified ZIF-8 metal-organic framework. This material serves as a highly sensitive near-infrared fluorescence sensing platform. Upon introducing mitoxantrone (MITX) as a fluorescence quencher, the material achieves highly selective and sensitive fluorescence detection of MITX. Mechanistic studies revealed that the fluorescence quenching of TCPP-His@ZIF-8 by MITX involves multiple mechanisms: the shift in absorption peaks confirms the presence of static quenching, while FRET is the primary mechanism responsible for the shortening of fluorescence lifetime, i.e., the dynamic quenching process. The inner filter effect (IFE) contributes partially to the reduction in apparent fluorescence intensity but is not the dominant factor. Under optimal experimental conditions, the sensor exhibits good linearity for MITX detection within the range of 0.125–14.0 μM, with a detection limit as low as 91.93 nM. Anti-interference and selectivity experiments demonstrated that common interferents and antibiotic analogues did not significantly affect detection. Furthermore, spiked recovery experiments in real samples such as tap water, milk, and human serum yielded satisfactory results, validating the reliability and potential of this method for practical applications.
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Innovative Food Science & Emerging Technologies
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2026
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IF 6.9
Junli Lv,Zhilong Ren,Jiali Zou,Nan Wang
Ferulic acid (FA) possesses significant therapeutic potential but is severely limited by its poor water solubility and instability. Although β-cyclodextrin (β-CD) inclusion complexation represents a promising strategy to overcome these limitations, the influence of host-guest molar ratios on complex properties remains inadequately understood. This study addresses this knowledge gap through an integrated experimental-computational approach, systematically investigating FA/β-CD complexes at three molar ratios (1:2, 1:1, 2:1) via structural characterization, molecular dynamics (MD) simulation, and comprehensive physicochemical evaluation. Successful complex formation at all ratios was confirmed by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and 1 H NMR spectroscopy analysis. MD simulations demonstrated that van der Waals forces primarily drive complex formation, while hydrogen bonds significantly contribute to structural stability. The 2:1 complex exhibited the highest loading efficiency of 21.5% and a 5.03-fold water solubility enhancement compared to pure FA, along with superior antioxidant activity as evidenced by IC 50 values of 0.024 mg/mL for DPPH and 0.042 mg/mL for ABTS radical scavenging. The 1:2 complex demonstrated the highest encapsulation efficiency of 93.3% and best light stability with only 8.53% loss after six days of exposure, and remarkably achieved the highest in vitro bioaccessibility of 93.6% after intestinal digestion. Conversely, the 1:1 complex exhibited optimal thermal stability with 95.18% retention after heating and the highest antibacterial activity against Escherichia coli with an inhibition zone diameter of 18.7 mm. These findings provide comprehensive theoretical guidance for selecting appropriate FA/β-CD molar ratios based on specific application requirements in food preservation, packaging materials, and pharmaceutical formulations.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2026
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IF 9.1
Dongcheng Li,Shenghu Wang,Huanji Song,Xianying Xu,Shihao Yang,Yuyang Dong,Yan Luo,Weiyang Lv
Efficient and sustainable Fenton-like systems are highly desired for degrading persistent pollutants. However, their development is often hindered by the slow redox kinetics and poor durability of single active sites in conventional metal-based catalysts. Herein, we developed a bimetallic Fe/Cu catalyst (FeCu-BN) with highly dispersed metal sites anchored on boron nitride (BN), which offered boosted activity and stability via the cooperative redox cycles between Fe and Cu sites. The FeCu-BN catalyst achieved nearly 100% removal of sulfamethoxazole (SMX) within 20 min through activating peroxymonosulfate (PMS), with a rate constant of 0.421 min − 1 significantly surpassing those of its monometallic counterparts and other advanced catalysts. Systematic experiments and theoretical calculations revealed that the Cu sites not only optimized the electronic structure of the Fe sites but also provided electrons extracted from pollutants to promote the Fe 2+ /Fe 3+ cycle, thereby triggering concerted radical and non-radical reactions for SMX elimination. Consequently, the FeCu-BN/PMS catalytic system operated stably over a wide pH range and exhibited exceptional tolerance to various inorganic ions and complex aqueous matrices. Furthermore, in a continuous flow membrane reactor, the system maintained efficient SMX removal over 20 h without significant performance loss. This work uncovered the synergistic redox cycling mechanism in bimetallic catalysts and offered valuable insights for designing dual-site catalysts for environmental remediation.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2026
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IF 4.8
Tingyuan Pang,Xuedi Xie,Guoxian Feng,Sijia Zheng,Zhenghao Li,Jia Li,Wenying Shu,Dongsheng Peng,Xiaoshun Jian,Ling Han,Meimei Zhang
Gynecological tumors pose a serious risk to female health, and traditional therapies have many limitations. Here, we developed a near-infrared light-triggered nanoplatform (UCNPs@SiO₂-MB@PDA) for synergistic photodynamic therapy (PDT) and photothermal therapy (PTT) of deep tumors. The nanoplatform takes the UCNPs as the core, wraps the silica shell loaded with the photosensitizer MB via the inverse microemulsion method, and finally modifies the surface of the PDA. Under 980 nm excitation, the red light emitted by the UCNPs can effectively activate MB to produce singlet oxygen through luminescence resonance energy transfer, achieving PDT. At the same time, the PDA shell can efficiently absorb the multiband emitted light of the UCNPs and convert it into thermal energy, achieving PTT with a photothermal conversion efficiency of 35.6%. In vitro cell experiments have shown that the material has good biocompatibility and can be effectively internalized by tumor cells. Under near-infrared light irradiation, its killing effect on HeLa cells was considerably better than that of monotherapy, and the cell survival rate decreased to 18.9% when the cells were exposed to a concentration of 200 μg/mL. The results of the live/dead cell staining further confirmed the excellent synergistic antitumor performance of these materials. This work offers a promising strategy for the precise and effective treatment of deep tumors.
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FISH & SHELLFISH IMMUNOLOGY
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2026
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IF 4.2
Ying-rong Jin,Chuan-yue Chen,Ze-kai Wang,Pan Qin,Zhi Liao,Xiao-lin Zhang,Xiao-jun Yan
C-type lectins (CTLs) play crucial roles in immune defense and feeding regulation, serving as key mediators of pathogen recognition and nutrient uptake in invertebrates. Although previous studies have demonstrated the involvement of CTLs in the immune response of Mytilus coruscus , their role in feeding regulation remains unclear. In this study, we identified a C-type lectin, designated MytiCTL17A , from M. coruscus and investigated its dual function in immune defense and feeding behavior. The purified recombinant MytiCTL17A binds to LPS and PGN. Additionally, it binds to both Gram-positive and Gram-negative bacteria, promoting bacterial agglutination. Notably, this agglutination can be inhibited by PAMPs. These findings confirm that rMytiCTL17A functions as a pattern recognition receptor (PRR), recognizing PAMPs, binding to bacteria, and promoting their agglutination, thereby playing a role in immune defense. Additionally, rMytiCTL17A also exhibits direct antibacterial activity. In feeding experiments, rMytiCTL17A can bind to microalgae and reduce the feeding rate of M. coruscus , indicating its involvement in the feeding process. Moreover, rMytiCTL17A can recognize carbohydrates (D-Man, D-Gal, Glu, Mal, and Suc) on the surface of microalgae and induce microalgal agglutination. Notably, this agglutination can be inhibited by D-Man, demonstrating its dependence on carbohydrate recognition. Thus, rMytiCTL17A also participates in feeding by recognizing microalgae through specific carbohydrates. This study highlights the multifunctionality of rMytiCTL17A and provides new insights into the roles of C-type lectins in bivalves, with potential implications for aquaculture in terms of enhancing disease resistance and feeding efficiency.
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