CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Wancun Zhang,Zhenzhen Tian,Pin Zhang,Lifeng Li,Zhidan Yu,Meng Sun,Kangbo Liu,Ligong Hou,Jie Zhang,Mengxin Zhang,Xianwei Zhang
Clinical practice has demonstrated that the absence of in vivo drug resistance monitoring strategies for neuroblastoma (NB) contributes to relapse rates exceeding 60 % among children with high-risk NB. These patients frequently miss the optimal therapeutic window for treatment, leading to poor prognoses and ultimately fatal outcomes. Herein, a novel strategy (APII-tFNA) was developed by targeting apurinic/apyrimidinic endonuclease 1 (APE1) and miR-514a for rapidly and spatially in vivo imaging of NB and monitoring NB drug resistance. Both APE1 and miR-514a have been rigorously validated as critical NB drug resistance markers at the clinical NB tissue level. APII-tFNA, the APE1 cyclically cleaved and miR-514a self-recycled programmable allosteric DNA nanomachine, operates via an AND-gated manner. APII-tFNA can hybridize with miR-514a, leading to the formation of the 514a-APII-tFNA complex. Subsequently, the apurinic/apyrimidinic site (AP site) within the 514a-APII-tFNA complex can be specifically cleaved by APE1, leading to spatial separation between fluorophore and quencher, and configurational changes in 514a-APII-tFNA. Simultaneously, APE1 and miR-514a can enter the next reaction cycle to generate more fluorescence signals, thereby enabling the highly sensitive detection of targets. Experimental results demonstrated that this responsive nanomaterial achieved a limit of detection (LOD, 3σ) of 0.25 pM for in vivo molecular imaging of NB drug resistance marker and was able to guide precise surgical excision with superior spatial specificity. This study presents a nanomaterial platform for in vivo molecular imaging of NB and monitoring NB drug resistance, with great potential for improving the survival rate of children with NB.
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高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Jia Feng,Jia Tu,Chunling Li,Dongyang Deng,Huihui Wang,Yongzhen Dong,Yiping Chen
Sensitive, rapid, and multiplexed detection of foodborne pathogens is critical for ensuring food safety, given the prevalence of multipathogen contamination in foods. Herein, we developed a polystyrene (PS) microsphere-enabled three-dimensional (color/size/number) sensing strategy and further established an oil-immersion microscopy and computer vision-integrated imaging biosensor for multiplexed detection of foodborne pathogens. This method employed differentially colored and sized microspheres as signal probes for pathogen encoding. By the integration of aptamer-binding reactions with the computer vision algorithm-based decode and counting process, the number of PS probes was correlated with the corresponding pathogen concentration. Meanwhile, the oil-immersion imaging system significantly enhanced microsphere image clarity and resolution, reducing method dependence on algorithmic and instrumental performance while improving applicability. This biosensor enables the simultaneous detection of multiple foodborne pathogens (e.g., Salmonella, Listeria monocytogenes, and Staphylococcus aureus) within 50 min at detection limits below 100 CFU/mL without DNA extraction, providing an intelligent platform for food safety assurance.
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高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Juan Wang,Yujia Ren,Baojian Huang,Chuanping Li,Shuai Qin,Shaojun Dong,Daoqing Fan
Exploring novel-type nanozymes with programmatically manipulated enzyme-mimicking activity for targets’ accurate identification and multimodal biosensing represents one of the most fascinating yet challenging research frontiers. Herein, taking DNAs as programmable biotemplates, we prepared bimetallic PtAg nanocluster-zyme via simple in situ reduction. Among them, the A20-templated PtAg nanozyme exhibited superior peroxidase-like (POD-like) activity, as confirmed by optical analyses, steady-state kinetics, and electron paramagnetic resonance spectroscopy, with •O2– and •OH identified as the primary reactive oxygen species. Taking Pt-AMP with the Pt–N4 coordination structure as representative, pluralistic DFT calculations were performed. Corresponding results manifested the superior affinity of nanozyme for H2O2 and TMB, the elongated O–O bond length of H2O2 and facilitated electron-transfer ability at the catalytic core. Subsequently, by harnessing the efficient catalytic oxidation of TMB and AR, we fabricated a colorimetric-photothermal-fluorescent trimodal sensing platform for assessing total antioxidant capacity and alkaline phosphatase activity. Superior to previous single/dual-modal biosensors, this work demonstrated significant advantages in sensitivity, reliability, and accuracy, and showed acceptable adaptability in diversified real matrices (human serum, tablets, and beverages). Furthermore, we fabricated target-responsive cascade logic circuits by virtue of the proper incorporation of trimodal signals and Boolean logic, and achieved the smart recognition of targets. This work not only provided a mild, simple, and efficient biotemplated approach for the programmable tuning of noble-metal nanozymes’ catalytic activity but also enlightened novel pathways for the operation of logic-empowered intelligent biosensors.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Yongzhen Dong,Aoting Gu,Chunling Li,Huihui Wang,Yang Zhou,Zhixiong Pi,Chengjia Jiang,Yiping Chen
A CbAgo-CRISPR/Cas12a-powered cascade amplification sensing strategy was proposed. • It combined CbAgo's precise cleavage with the Cas12a's robust trans-cleavage activity. • An amplified fluorescent biosensor was developed for detecting Salmonella Typhimurium . • The proposed biosensor demonstrated comparable analytical performance to qPCR assay. Sensitive and accurate detection of foodborne pathogens constitutes a critical imperative for safeguarding food safety. Herein, we proposed a Clostridium butyricum argonaute (Cb A go)-CRISPR/ C as12a-powered synergistic amplification strategy and developed a DNA amplification-free f luorescent ( ACF ) biosensor for ultrasensitive detection of Salmonella Typhimurium . In this biosensor, magnetic beads-phage conjugates enabled the selective capture of viable Salmonella Typhimurium , followed by rapid DNA extraction within 5 min using commercial lysis buffers. A synergistic signal amplification strategy was designed for efficient biosensing by utilizing the primary cleavage product (tDNA) of the CbAgo system as a molecular bridge to integrate CbAgo and Cas12a systems. Specifically, CbAgo performed primary cleavage of target DNA to generate tDNA fragments. These tDNA subsequently activated the CRISPR/Cas12a system, initiating its trans-cleavage activity to degrade fluorescent reporter probes for quantitative analysis. This tDNA-mediated integration synergizes CbAgo's precision cleavage capability with Cas12a's efficient trans-cleavage activity, enabling exponential amplification. Under optimized conditions, the ACF biosensor exhibited a linear detection range of 10 2 –10 7 CFU/mL and a detection limit of 24.8 CFU/mL for Salmonella Typhimurium without DNA amplification steps. Moreover, the ACF biosensor demonstrated comparable analytical performance to the qPCR assay and achieved satisfactory results in the detection of Salmonella Typhimurium in 20 food samples, highlighting its potential as an ultrasensitive tool for foodborne pathogen monitoring. Download: Download high-res image (107KB) Download: Download full-size image
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高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Jun Xiong,Ke-Ke Chen,Neng-Bin Xie,Tong-Tong Ji,Si-Yu Yu,Feng Tang,Conghua Xie,Yu-Qi Feng,Bi-Feng Yuan
DNA methylation (5-methylcytosine, 5mC) is the most prevalent epigenetic modification that is predominantly found in CG dinucleotides in mammalian genomes. In-depth investigation of the functions of 5mC heavily relies on the quantitative measurement of 5mC at single-base resolution in genomes. Here, we proposed a methyltransferase-directed labeling with APOBEC3A (A3A) deamination sequencing (MLAD-seq) method for the single-base resolution and quantitative detection of 5mC in DNA. In MLAD-seq, a mutant of DNA methyltransferase, M.MpeI-N374K, is utilized to selectively transfer a carboxymethyl group to the 5 position of cytosine in the CG dinucleotide to form 5-carboxymethylcytosine (5camC) using carboxy-S-adenosyl-l-methionine (caSAM) as the cofactor. After A3A treatment, 5camC is resistant to the deamination and base pairs with guanine. Thus, the cytosines in CG sites are read as C in sequencing. On the contrary, the methyl group in 5mC inhibits its carboxymethylcytosine by M.MpeI-N374K and therefore is readily deaminated by A3A to produce thymine that pairs with adenine and is read as T in sequencing. The differential readouts from C and 5mC in the MLAD-seq enable the single-base resolution mapping of 5mC in CG sites in DNA. With the developed MLAD-seq method, we observed the hypermethylation in the promoter region of retinoic acid receptor β (RARB) gene from human nonsmall cell lung tumor tissue. Compared to harsh reaction conditions in bisulfite sequencing that could lead to significant degradation of DNA, the whole procedure of MLAD-seq is carried out under mild conditions, which will avoid DNA damage. Thus, MLAD-seq is more suitable in the scenario where only limited input DNA is available. Taken together, the MLAD-seq offers a valuable tool for bisulfite-free, single-base resolution and quantitative detection of 5mC in limited DNA.
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xiaoyu Su,Zhiyu Chen,Huan Wang,Lei Yuan,Kaiyi Zheng,Wen Zhang,Xiaobo Zou
A ratiometric electrochemical immunosensor, based on DNA tetrahedron nanostructure (DTNS), is introduced for vegetable phoxim determination. DTNS spontaneously adheres onto gold-nanoparticle-modified electrode and forms stable three-dimensional structure, providing plenty of binding sites to the built-in reference, methylene blue (MB). Monoclonal antibody (m-Ab) is vertically linked onto DTNS vertex, selectively responses antigenic phoxim, and promotes the target signal of I PHO . Thus, a ratiometric indicator, I PHO / I MB , is sensibly established with the target signal ( I PHO ) and the reference signal ( I MB ). Modifications, mechanisms and advances of the proposed method are subsequently examined with morphological methods and electrochemical experiments. This method brings considerable advances in analytical behaviors. The ratiometric signal presents better performance than solo system in repeatability and long-time stability. As-fabricated sensor presents wide dynamic range as 0.1∼30 μg/L, and limit of detection is well defined as 0.003 μg/L (S/N = 3). Finally, this method is verified with real-vegetable-sample analysis, certified HPLC and recovery test.
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高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Qian Chen,Meijuan Du,Xueqin Xu
Di(2-ethylhexyl) phthalate (DEHP) is an endocrine disrupting compound that is often used as a plasticizer and tends to migrate in the environment and accumulates in the human body causing harm to humans. Thus, a simple, label-free, enzyme-free, high selective and ultrasensitive electrochemical aptasensor has been constructed for detection of DEHP based on the self-assembly of DNA nanostructure signal amplification strategy in this paper. The DNA-junction nanostructure is made of hairpin 1 (HP1), hairpin 2 (HP2), hairpin 3 (HP3) and quarter sequence (QS). Without DEHP, the electrochemical detection response is very low. Only when the aptamer binds to the target and detaches from the electrode surface, the DNA-junction is trapped on the electrode surface, enhancing the electrochemical signal. This is because the DNA-junction nanostructure is rich in guanine, which is able to adsorb more methylene blue (MB) for the purpose of enhancing the detection signal. The linear detection range of the obtained aptasensor is 0.1 ng mL −1 to 5000 ng mL −1 and the limit detection is 0.04 ng mL −1 . Finally, the proposed aptasensor has been applied to analyze DEHP in real samples, laying the foundation for its utilization in environmental water samples and food safety.
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高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Qingbin Yuan,Yi Wang,Shangjie Wang,Ruiqing Li,Junlu Ma,Yijing Wang,Ruonan Sun,Yi Luo
Despite severe threats of extracellular antibiotic resistance genes (eARGs) towards public health in various environments, advanced studies have been hindered mainly by ineffective extracellular DNA (exDNA) extraction methods, which is challenged by trace levels of exDNA and inference from abundant coexisting compounds. This study developed a highly selective exDNA extraction method based on molecular imprinting technology (MIT) by using adenine as the template for the first time. Results suggested that adenine imprinted beads were rough spheres at an average size of 0.39 ± 0.07 μm. They effectively adsorbed DNA in the absence of chaotropic agents, with superior capacity (796.2 mg/g), rate (0.0066/s) and regarding DNA of variable lengths, even the ultra-short DNA (<100 bp). They were also highly selective towards DNA, circumventing the interference of competitive compounds' interference. These properties contribute to efficient exDNA extraction (71 %–119 %) from various environmental samples. Specifically, adenine imprinted beads enabled significantly higher extraction rates of eARGs from river, air and vegetable samples (69 %–95 %) compared to that by commercial DNA extraction products (16 %–62 %). The adenine imprinted beads-based method reveals underestimated eARG levels in the environment and the corresponding risks, and thus will thus be a powerful tool for advanced exDNA research.
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高影响因子
Small
·
2022
·Vol. 18
·期 37
·2201925
·
IF 15.153
Lingxiao Liu,Cihui Luo,Jinhuan Zhang,Xiao He,Ying Shen,Bing Yan,Yu Huang,Fan Xia,Lei Jiang
During the past few decades, bio-inspired nanochannels have been well developed and applied in biosensing, energy transfer, separation, and so on. Here, inspired by the synergistic effect of biological nanopores, biomimetic solid-state nanochannels with hydrophilic DNA probes at the inner wall (DNA@IW Hydrophilic ) and hydrophobic coating at the outer surface (None@OS Hydrophobic ) are designed. To demonstrate their prompted sensing properties, Hg 2+ and its specific probe are selected as target and hydrophilic DNA probes, respectively. Compared with the traditional solid-state nanochannels with hydrophilic probes distributed on both the inner wall and outer surface, the nanochannels with DNA@IW Hydrophilic +None@OS Hydrophobic significantly decrease the limit of detection (LOD) by 10 5 -fold. The obvious improvement of sensitivity (with LOD of 1 nM) is attributed to the synergistic effect: None@OS Hydrophobic results in the nanochannel's effective diameter decrease and DNA@IW Hydrophilic induces a specific sensing target. Meanwhile, nanomolar detection of Hg 2+ in human serum and in vivo fish muscle are achieved. Through molecular dynamics simulation, the synergistic effect can be confirmed by ion fluxes increasement; the relative carbon nanotube increases from 135.64% to 135.84%. This work improves the understanding of nanochannels’ synergistic effect and provides a significant insight for nanochannels with improved sensitivity.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Yan Liu,Shao-Hua Wu,Xing-Yuan Du,Jian-Jun Sun
Surface-enhanced Raman scattering (SERS) biosensing has attracted great attention due to its multiplexed capability, high sensitivity, and rapid readout. In this study, plasmonic Ag nanocube (AgNC) enhanced SERS biosensing platform was proposed by coupling nicking endonuclease-assisted target recycling signal amplification with heated electrode for sensitive detection of DNA species related to oral cancer. SERS tags were manufactured by signal DNA (sDNA) conjugating onto the surfaces of AgNCs, and 4-mercaptobenzoic acid (4-MBA) assembled on the surfaces of AgNC/sDNA as a Raman reporter. The sharp feature of edges and corners of AgNCs with high electromagnetic field provided SER hot spots with high quality, and stronger SERS signal could be achieved. In addition, the activity of nicking endonuclease Nt.BstNBI could be greatly enhanced by elevating the temperature of the surface of the heated Au electrode (HAuE) during Nt.BstNBI-assisted target recycling amplification process, resulting in dramatic amplification of target DNA detection. All of these contributed to the high sensitivity of the developed SERS biosensor. A limit of detection (LOD) of 3.1 fM (S/N = 3) could be obtained with an electrode temperature of 45 °C. This proposed SERS biosensor was anticipated to act as a promising biosensing platform for early clinical diagnosis of oral cancer.
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高影响因子
ACS Applied Bio Materials
·
2021
·
IF 0
Junyang Chen,Mengke Wang,Ying Bao,Xiaolei Xie,Yixin Nie,Yuntai Lv,Xingguang Su
The rapid and accurate monitoring of viral genes plays an important role in the area of disease diagnosis, biomedical research, and food safety. Herein, we successfully designed a sensing system that combined the technologies of target DNA recycling amplification, magnetic separation, and in situ formation of fluorescent copper nanoclusters (CuNCs) for viral DNA analysis. In the presence of target viral DNA (tDNA), a large quantity of output DNA (oDNA) was produced from hairpin DNA (hDNA) through an exonuclease III-assisted target recycling amplification strategy. Magnetic beads (MBs) labeled with capture DNA (cDNA) were hybridized with oDNA, and the partially complementary oDNA served as a bridge that could link AT-rich dsDNA on the surface of MBs, which led to a decrease of AT-rich dsDNA in solution after magnetic separation. On account of the lack of AT-rich dsDNA as a template in solution, in situ formation of fluorescent CuNCs was blocked, which resulted in a decrease in the fluorescence intensity at 590 nm. Therefore, taking advantage of one-step magnetic separation and in situ formation of CuNCs, the target viral DNA was sensitively and specifically detected in a linear range from 5 pM to 5 nM with a detection limit of 1 pM. The MB-based platform was not only reusable but also achieved magnetic separation, which could eliminate interferences in complex samples. The assay combining the MB-based probe with fluorescent CuNCs provided a universal, label-free, and reusable platform for viral DNA detection.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Lin Liu,Daohong Wu,Shuang Zhen,Kaiqing Lu,Xinyao Yi,Zhifang Sun
This work reports on a simple and sensitive electrochemical strategy for the detection of telomerase extracted from HeLa cells. It was based on the signal amplification of in-situ performed streptavidin-biotin-DNA-biotin (SA-biotin-DNA-biotin) networks. Specifically, the DNA primer immobilized on a gold electrode was elongated by telomerase. After the hybridization of biotin-DNA-biotin to the extended primer, the in-situ formation of SA-biotin-DNA-biotin networks was initiated on the electrode surface within 10 min through the SA-biotin interactions. The resulting networks created an insulating layer, thus hampering the electron transfer and causing the significant increase in the electrochemical impedance. Telomerase extracted from 2 HeLa cells can be readily measured. The method showed a linear relationship in the range of 50 ∼ 5000 cells/mL. The signal-amplified strategy can be applied to develop novel sensing platforms for the detection of different biomarkers.
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高影响因子
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