Food Analytical Methods
·
2013
·Vol. 7
·期 1
·21-30
·
IF 1.969
Xie Qilong,Liu Shuhui,Fan Yingying,Zhang Xiaoke
A dispersive liquid–liquid microextraction (DLLME) method coupled to high-performance liquid chromatography was developed for the analysis of α-tocopherol in grain samples. The DLLME parameters including the type and volume of extractants, the volume of disperser and the addition of salt were examined. The optimized DLLME procedure consisted in the formation of a cloudy solution promoted by the fast addition to the sample (5 mL of saponified sample solution diluted with 5 mL of water) of a mixture of carbon tetrachloride (extraction solvent, 80 μL) and ethanol (dispersive solvent, 200 μL) without the addition of salt, followed by shaking for 5 min and centrifuging for 3 min at 5,000 rpm. Intra- and inter-day repeatability expressed as % RSD were 3.5 and 7.6 %, respectively. The limit of detection and the limit of quantification were 1.9 and 6.3 μg L −1 . The comparison of this method with the national standardized extraction method, supercritical carbon dioxide extraction, accelerated solvent extraction, and conventional heat-reflux extraction indicates that the DLLME was accurate (no significant differences at the 0.05 % probability level), high efficient, low organic solvent-consuming, and low cost. This procedure was successfully applied to 42 samples of 14 types of purple wheat, for which the content of α-tocopherol exhibited a significantly negative correlation with the pigment content measured by a spectrophotometer. The recovery rates ranged from 90.5 to 103.7 %.
✓ 已验证
产品关联
DOI
高影响因子
Food and Bioprocess Technology
·
2015
·Vol. 8
·期 9
·1958-1973
·
IF 2.691
Xu Enbo,Wu Zhengzong,Long Jie,Wang Fang,Pan Xiaowei,Xu Xueming,Jin Zhengyu,Jiao Aiquan
In order to investigate the effect of thermostable α-amylase on the rice physicochemical properties, free/bound phenolics, and antioxidant capacities during extrusion, seven different gradients of enzyme concentration from 0 to 6 ‰ (db of starch) were used for both hulled and whole rice. For rice treated with the same extrusion conditions, special mechanical energy (SME) input changed slightly in the presence of fiber fraction (bran/husk), while decreased greatly with increasing α-amylase levels due to the reduced viscosity and friction between mechanical elements and materials. Huge physicochemical modifications were achieved in expansion ratio (ER), bulk density (BD), water absorption/solubility indices (WAI/WSI), and viscosity via enzymatic liquefaction. The total phenolic content (TPC) retention increased dramatically (93.06 and 86.33 % for hulled and whole rice, respectively) with increasing enzyme concentrations to 6 ‰, indicating that a milder reaction environment was created with less thermal-mechanical impact on phenolics. By linear analysis, the TPC of extruded rice hulled or not was strongly and positively related with BD ( R = 0.9185 or 0.9719) and WSI ( R = 0.9719 or 0.8857), whereas negatively related with SME ( R = −0.9460 or −0.8906). Besides, the free/bound phenolics combined with antioxidant activities (1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), and ferric reducing antioxidant power (FRAP)) were all improved significantly by the separate/synergetic addition of enzyme and bran/husk used as, respectively, the “special protective agent” and rich source of bioactive compounds. In summary, thermostable α-amylase could achieve many beneficial effects on both physicochemical properties and antioxidant activities of rice extruded with/without fiber for promoting the development of cake making, wine brewing, infant food industries, or animal husbandry.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2017
·
IF 3.012
Anran Liu,Haitao Shan,Mengyao Ma,Li Shangguan,Kunpeng Jiang,Minke Shi,Yuewu Zhao,Songqin Liu,Shufeng Li
Dysregulation of human tumor necrosis factor α (TNF-α) production has been implicated in a variety of human diseases. Serum level of TNF-α is frequently detected in clinical diagnosis of cancer patients. Herein, we present an ultrasensitive photoelectrochemical (PEC) sandwich immunosensor for TNF-α detection by utilizing anti-TNF-α nanobody (Nb) orientedly immobilized to the CdS and Mn cosensitized TiO 2 nanorod arrays electrode. Using highly oriented Nb as receptor molecules and a secondary anti-TNF-α nanobody conjugated with ZnS nanoparticles as the signal amplification element, the PEC immunosensor for TNF-α was developed by monitoring the changes in the photocurrent intensity of the electrode resulting from immunoreaction. The proposed method has the linear range from 2.0 pg/mL to 200 ng/mL and the detection limit was 1.0 pg/mL. The method also reveals high specificity to detect TNF-α, with acceptable intra-assay precision and excellent stability. The results for the detection of ten serum samples from lung cancer patients and normal human have good agreement with the clinical data.
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Taotao Xu,Junhua Li,Jun Cao,Wenxia Gao,Li Li,Bin He
Poly(pseudo)rotaxane (PPR) nanoparticles was facilely prepared using poly(ethylene glycol) (PEG) modified with protoporphyrin (PpIX) and α-cyclodextrin (α-CD) via host-guest interaction, the effect of α-CD number on the nanoparticle properties was investigated. The PEG with protoporphyrin (PEG-PpIX) end capping was synthesized via coupling reaction and the poly(pseudo)rotaxane nanoparticles with different amount of α-CD were fabricated by host-guest interaction between mPEG-PpIX and α-CDs. The final product was characterized by nuclear magnetic spectrum ( 1 H NMR), X-ray diffraction (XRD), atomic force microscope (AFM) and dynamic light scattering (DLS). The results showed that the poly(pseudo)rotaxane nanoparticles with uniform spherical shape was successfully prepared and doxorubicin (DOX) could be efficiently encapsulated in the nanoparticles. The amount of α-CDs in poly(pseudo)rotaxane nanoparticles was proportional to micellar size and drug release rate. The nanoparticles with higher α-CD number showed better anticancer efficacy in half maximal inhibitory concentration (IC 50 ) test. The cell internalization efficiency of DOX-loaded poly(pseudo)rotaxane nanoparticles could be further improved by lowering the α-CD number to receive smaller nanoparticle size.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR MODELING
·
2017
·Vol. 23
·期 8
·1-11
·
IF 1.425
Sun Jiachen,Dong Shengjie,Wu Yueting,Zhao Hui,Li Xia,Gao Wenyuan
It is known that inhibiting α-amylase, an important enzyme in digestion of starch and glycogen, is a useful strategy for treating disorders in carbohydrate uptake. Two natural components distributed in many fruits and plants, oleanolic acid and ursolic acid, are endowed with important pharmacological activities and wide therapeutic possibilities. Until now, only a tiny fraction of their applications have been identified and exploited. Our in vitro inhibition studies demonstrated that oleanolic acid and ursolic acid non-competitively inhibit the activity and function of human salivary α-amylase. The molecular simulations revealed that oleanolic acid and ursolic acid interact with amino acid residues within the binding pocket of human salivary α-amylase, among which the side chain of Arg195 and Asp 197 was supposed to be important in imparting the inhibitory activity of triterpenoids. The present work will provide meaningful information for future development of functional drugs for the treatment of disorders in carbohydrate metabolism.
This work is valuable for providing a deeper insight into the interaction mechanism of oleanolic acid and ursolic acid with α-amylase
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHROMATOGRAPHIA
·
2016
·Vol. 79
·期 5
·327-333
·
IF 1.332
Peng Xiaofeng,Wang Huawen,Yang Binbin,Zhan Xin,Wu Yiwei
To improve the sensitivity of capillary electrophoresis, field-amplified sample injection (FASI) was developed to determine bisphenol A (BPA), α -naphthol ( α -NAP) and β -naphthol ( β -NAP) in drinks and lake water. Parameters (sample matrix, concentration of NaCl, water plug length, sample injection time and voltage) affecting FASI have been systematically investigated. Under optimum conditions, the sensitivity was improved 17.1-, 15.8- and 9.9-fold for BPA, α -NAP and β -NAP, respectively. The detection limits of BPA, α -NAP and β -NAP were 0.071, 0.038 and 0.081 μg mL −1 and the proposed method has been successfully applied to detect BPA, α -NAP and β -NAP in drinks and lake water with recoveries of 82.0–109.3 %.
✓ 已验证
DOI
高影响因子
Chinese Medicine
·
2024
·Vol. 19
·期 1
·1-17
·
IF 5.3
Gao Jiaming,Guo Hao,Li Junmei,Zhan Min,You Yue,Xin Gaojie,Liu Zixin,Fan Xiaodi,Gao Qinghe,Liu Jianxun,Zhang Yehao,Fu Jianhua
Background Buyang Huanwu Decoction (BYHWD) is a traditional Chinese medicine to treat the syndrome of qi deficiency and blood stasis. Platelets play an important role in regulating thrombus and inflammation after ischemic injury, studies have shown that BYHWD regulate myocardial fibrosis and exert anti-inflammatory effects through IL-17 and TLR4 pathways, but the mechanism of platelet activation by BYHWD in stable coronary heart disease is still unknown. In the present study, model of left anterior descending coronary artery ligation was applied to investigate the mechanisms of BYHWD on modulating platelets hyperreactivity and heart function after fibrosis of ischemic myocardial infarction (MI). Methods Myocardial infarction model was constructed by ligation of the left anterior descending coronary artery. The rats were randomly divided into five groups: sham, model, MI with aspirin (positive), MI with a low dosage of BYHWD (BYHWD-ld) and MI with a high dosage of BYHWD (BYHWD-hd) for 28 days. Results Coronary artery ligation prominently induced left ventricle dysfunction, increased cardiomyocyte fibrosis, which was accompanied by platelets with hyperreactivity, and high levels of inflammatory factors. BYHWD obviously reversed cardiac dysfunction and fibrosis, increased the thickness of the left ventricular wall, and inhibited aggregation ratio and CD62p expression. BYHWD restored the mitochondrial respiration of platelets after MI, concomitant with an increased telomere expression and decreased inflammation. According to the result of transcriptome sequencing, we found that 106 differentially expressed genes compared model with BYHWD treatment. Enrichment analysis screened out the Ras-related protein Rap-1 (Rap1) signaling pathway and platelet activation biological function. Quantitative real-time PCR and Western blotting were applied to found that BYHWD reduced the expression of Rap1/PI3K-Akt/Src-CDC42 genes and attenuated the overactivity of PI3 kinase/Rap1/integrin α(IIb)β(3) pathway. Conclusion BYHWD reduced inflammation and platelet activation via the PI3 kinase/Rap1/integrin α(IIb)β(3) pathway and improved heart function after MI. Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
·
2024
·
IF 5.8
Lingjiao Zhan,Chaozhong Wu,Fuqin Zhang,Juan Wang,Haotian Liu,Yutong Chen,Shuwei Yao,Yunzhu Ma,Wensheng Liu
The coordinated regulation for α-Al 2 O 3 and mullite structures in the alumina-mullite duplex fiber poses a challenging yet crucial task in achieving a fiber with exceptional high-temperature properties. In this study, Fe element was added into the alumina-mullite fibers in the form of iron sol and ferric nitrate solution, respectively, with the aim of obtaining a fiber consisting of fine mullite grains embedded with nano α-Al 2 O 3 grains. The results revealed that the addition of Fe element significantly enhances the crystallization process of mullite in alumina-mullite fibers. Specifically, adding iron sol resulted in the reduce of α-Al 2 O 3 formation temperature to 1300 °C, while introducing Ferric nitrate solution promoted the formation of mullite phase. Simultaneous introduction of 1.3 wt% iron sol and 1.3 wt% Ferric nitrate solution contributed to achieve alumina-mullite fiber with tensile strength of 2.06 GPa. In addition, the influence mechanism of Fe source on the phase transformation and microstructures of alumina-mullite fiber were discussed.
✓ 已验证
产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2017
·
IF 5.951
Youping Zhou,Xijie Yin,Hubiao Yang,Jing Su,Huimin Yu,Yan Wang,Shuixiu Zhou,Saša Zavadlav
The chemical linkage between α-cellulose and lignin in plant cell walls has long been a controversial topic and crucial to devising effective strategies for sustainable biomass and bioenergy utilization. In this new contribution, we surveyed 80 Poaceae (Gramineae) species grown in tropical Hainan Island (China) to test the hypothesis that the presence of oxygen-containing chemical linkage in Poaceae species is widespread. Our innovative natural abundance oxygen isotopic analysis allowed us to infer that more than 1/3 of the species investigated has chemical (ether) linkages between α-cellulose and lignin in their leaf cell walls, with a species-specific 2–89 oxygen-containing bonds for every 1000 glucose units. However, the presence of such linkage appears to be phylogeny-dependent. On average, species of C3 photosynthetic mode are found to have more extensive oxygen-containing linkages than those of C4 photosynthetic mode. Our finding challenges the conventional view that no chemical bonds between α-cellulose and lignin are present in higher plant cell walls and calls for new strategies for further understanding of the chemical linkage between the two major constituents of cell walls. This is especially important in the context of renewed and growing interests in biomass, bioenergy, and plant cell wall structure studies.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Yaping Li,Yongqi Yang,Chunmeng Zhong,Dan Xiao,Cuisong Zhou
Sensitive detection of resistance mutation T790 M is of great significance for early diagnosis and prognostic monitoring of non-small-cell lung cancer (NSCLC). In this paper, we showed a highly sensitive detection strategy for T790 M using a three-level characteristic current signal pattern in an α-hemolysin nanopore. A probe was designed that formed a C–T mismatched base pair with wild-type/P and a T–T mismatched with the T790M/P. The T790M/P produced a unique three-level characteristic current signal in the presence of mercury ions(II): first, T790M–Hg2+–P entering the vestibule of α-HL under the transmembrane potential and overhang of probe occupying the β-barrel, then probe unzipping from the T790M/P, T790 M temporally residing inside the nanocavity due to the interaction with Hg(II), and finally T790 M passing through the β-barrel. The blocking current distribution was concentrated with a small relative standard deviation of about 3%, and the signal peaks of T790 M and wild-type can be completely separated with a high separation resolution of more than 2.5, which achieved the highly sensitive detection of T790 M down to 0.001 pM (confidence level P 95%) with a linear range from 0.001 pM to 1 nM in human serum samples. This highly sensitive recognition strategy enables the detection of low abundance T790 M and provides a method for prognostic monitoring in NSCLC patients.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ling Li,Yanxin Huang,Qianqian Ding,Danli Wang,Tinglan Yuan,Gongshuai Song,Hyunbin Seong,Jinyan Gong
Milk protein and hyaluronic acid (HA) at different mass ratios were systemically characterized using the Maillard reaction. Combining FTIR and molecular docking results, both conjugates, α-casein-HA (α-CN-HA) and β-lactoglobulin-HA (β-Lg-HA), were prepared via hydrogen-bond interactions. Binding of HA promotes the structural folding of milk proteins and alters their secondary structures. The α-CN-HA and β-Lg-HA conjugates exhibited significantly improved solubility thermal and antioxidant activity. The functional properties of the conjugates were modulated by varying the ratio of milk proteins to HA. The α-CN:HA 1:2 ratio had the highest denaturation temperature (110.1 °C). For β-Lg-HA, the highest denaturation temperature (112.1 °C) was observed at a 1:4 ratio. Antioxidant capacity increased with decreasing HA content; the highest value was observed at a mass ratio of 3:1 for both conjugates. Our findings suggest that Maillard reaction improves the physiological and functional properties of milk proteins and HA conjugates.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Today
·
2024
·
IF 13.2
Hongbing Liu,Muse Ji,Yi Qin,Yin Sun,Hanxun Wang,Peifu Xiao,Jiansong Zhao,Yaxin Deng,Zhen Zhang,Jingxin Gou,Tian Yin,Haibing He,Guoliang Chen,Xing Tang,Yu Zhang
Cytokine storm and pulmonary fibrosis caused by Coronavirus Disease 2019 (COVID-19) are the two major culprits threatening human life and health. Herein, we engineered a novel disulfide-bridged carrier-free prodrug self-assembled nanoparticles (DSSL NPs) composed of Dexamethasone (Dex) and α-linolenic acid (LNA) for synergistic inhibition of cytokine storm and pulmonary fibrosis. DSSL NPs possess the capability to selectively target injured lung tissue and precisely release drugs after intravenous administration. Consequently, a single administration of DSSL NPs effectively mitigated pro-inflammatory cytokines and tissue damage in mice with lipopolysaccharide (LPS)-induced cytokine storm by blocking the TNF, NF-κB, and Toll-like receptor signaling pathways. Moreover, long-term administration of DSSL NPs alleviated the abnormal fibroblast activation and collagen deposition in mice with LPS-induced pulmonary fibrosis by inhibiting the Wnt-PCP signaling pathway without obvious Dex-related side effects. Together, DSSL NPs achieved the effect of ‘killing two birds with one stone’, presenting a safe and reliable strategy for comprehensive treatment for COVID-19-related diseases.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）