ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Yuhang Liu,Yi Zhang,Pran Gopal Karmaker,Yuting Tang,Lilei Zhang,Feng Huo,Ya Wang,Xiupei Yang
The misuse of organic pollutants such as nitrofuran antibiotics (NFAs) and 2,6-dichloro-4-nitroaniline (DCN) has become a hot topic of global concern, and developing rapid, efficient, and accurate techniques for detecting NFAs and pesticides in water is a major challenge. Here, we designed a novel lead-based anion 2D metal–organic framework (MOF){[(CH3)2NH2]2[Pb(TCBPE)(H2O)2]}n (F3) with interlocking structures, in which TCBPE stands for 1,1,2,2-tetra(4-carboxylbiphenyl)ethylene. Powder X-ray diffraction and thermogravimetric analysis revealed that F3 has excellent chemical and solvent stability. It is worth noting that F3 has a grinding discoloration effect. The solvent-protected grinding approach achieved F3B with a high quantum yield (QY = 73.77%) and blue fluorescence, while the direct grinding method produced F3Y with a high quantum yield (QY = 37.27%) and yellow–green fluorescence. Importantly, F3B can detect NFAs in water rapidly and sensitively while remaining unaffected by other antibiotics. F3Y can identify DCN in water quickly and selectively while remaining unchanged by other pesticides. F3B demonstrated high selectivity and rapid response to NFAs at a limit of detection (LOD) as low as 0.26 μM, while F3Y indicated high selectivity and responded quickly to DCN in water at an LOD as low as 0.14 μM. The method was successfully applied to detect NFAs in actual water samples of the fish tanks and ponds as well as the pesticide DCN in soil samples. The recovery rates were 97.0–105.15% and 102.2–106.48%, and the relative standard deviations were 0.63–1.45% and 0.29–1.69%, respectively. In addition, F3B and F3Y can be made into fluorescent test papers for the visual detection of NFAs and DCN, respectively. Combined with experiments and density functional theory calculations, the mechanism of fluorescence quenching of MOFs by target analytes was also revealed.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Sheng Chen,Jiayi Fan,Mengya Lv,Chenfeng Hua,Gaolin Liang,Shusheng Zhang
Hydrogen sulfide (H2S), as the third endogenous gasotransmitter, is closely associated with various physiological and pathological processes, whereas many aspects of its functions remain unclear. Effective tools for the accurate detection of H2S in living organisms are urgently needed. We herein reported an internal standard assisted surface-enhanced Raman scattering (SERS) nanoprobe for ratiometric detection of H2S in vitro and in living cells based on the reduction of nitros with H2S. This nanoprobe consists of an internal standard (4-mercaptobenzonitrile, MPBN) embedded core-molecule-shell Au nanoflower (Au@MPBN@Au) as the high plasmonic active SERS substrate and the 4-nitrothiophenol (4-NTP) molecule immobilized on the surface as the H2S recognition unit. With the addition of H2S, the nitros peak (1329 cm–1) decreased. Meanwhile, three obvious new peaks appeared at 1139, 1387, and 1433 cm–1, which were related to the vibration of the dimerized product 4,4′-dimercaptoazobisbenzene (DMAB) of 4-aminothiophenol (4-ATP). However, the peak intensity at 2223 cm–1 derived from MPBN was not influenced by the outer environment. Thus, the H2S level was able to be determined based on the ratio of two peak intensities (I1139/I2223) with a detection limit as low as 0.24 μM. Notably, we have proved that SERS nanoprobe Au@MPBN@Au@4-NTP could ratiometrically image both the endogenous and exogenous H2S in living cells. We anticipate that Au@MPBN@Au@4-NTP could be applied for the study of H2S-related physiological function in the future.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bioengineering & Translational Medicine
·
2022
·Vol. 8
·期 2
·e10414
·
IF 10.684
Peng Luo,Jiarui Fang,Dazhi Yang,Lan Yu,Houqing Chen,Changging Jiang,Rui Guo,Tao Zhu,Shuo Tang
Bone injury caused changes to surrounding tissues, leading to a large number of osteoclasts appeared to clear the damaged bone tissue before bone regeneration. However, overactive osteoclasts will inhibit bone formation. In this study, we prepared methacrylylated gelatin (GelMA)-based hydrogel to co-crosslink with OP3-4 peptide, a receptor activator of NF-κB ligand (RANKL) binding agent, to achieve the slow release of OP3-4 peptide to inhibit the activation of osteoclasts, thus preventing the long-term existence of osteoclasts from affecting bone regeneration, and promoting osteogenic differentiation. Moreover, CXCL9 secreted by osteoblasts will bind to endogenous VEGF and inhibit vascularization, finally hinder bone formation. Thus, anti-CXCL9 antibodies (A-CXCL9) were also loaded in the hydrogel to neutralize excess CXCL9. The hydrogel slow released of OP3-4 cyclic peptide and A-CXCL9 to simultaneously inhibiting osteoclast activation and promoting vascularization, thereby accelerating the healing of femur defect. Further analysis of osteogenic protein expression and signal pathways showed that the hydrogel may be through activating the AKT-RUNX2-ALP pathway and ultimately promote osteogenic differentiation. This dual-acting hydrogel can effectively prevent nonunion caused by low vascularization and provide long-term support for the treatment of bone injury.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Xiaoxiao Li,Yu Wang,Jing Wu,Zhengyu Jin,Lubbert Dijkhuizen,Maher Abou Hachem,Yuxiang Bai
Solid-liquid thermo-reversibility, lacking in raw starch is an important property for gels allowing repeated thermal processing in food . In this work, the Thermoproteus uzoniensis 4-α-glucanotransferase (TuαGT) was used to modify five different starches from potato, corn , rice, wheat, and pea and the thermo-reversibility of the modified starch gels was evaluated by rheological assay. Only modified potato starch (MPS) exhibited thermo-reversibility, which prompted us to perform detailed analyses of the structural and gel properties of this product. The enzymatic modification resulted in a marked decrease in amylopectin side chains with a degree of polymerization (DP) < 23, while the contents of side chains with DP 23–43 increased. These data indicated that TuαGT preferentially extended the outer chains of amylopectin using amylose as a donor, which resulted in 17% increase in the amylopectin molecular weight to 3.0 × 10 7 g/mol. Notably, MPS displayed a delayed retrogradation and improved stability in four freeze-thaw cycles (−70 °C and 25 °C) as compared to the native starch. These analyses supported a model to explain the TuαGT catalyzed conversion of potato starch to a thermo-reversible product. Importantly, the MPS thermo-reversible 6% (w/v) gel showed comparable rheological properties to a 1.5% (w/v) κ-carrageenan hydrocolloid , highlighting the potential of MPS as a substitute to κ-carrageenan and other hydrocolloids of emerging health concerns. This work offers a starch based thermo-reversible gel and a methodological framework to broadens the use of starch into novel application as a food ingredient.
✓ 已验证
产品关联
DOI
高影响因子
Green Energy & Environment
·
2022
·
IF 12.781
Yang Zhou,Qiang Zeng,Hongyan He,Kejia Wu,Fuqiao Liu,Xuehui Li
In order to better understand the specific substituent effects on the electrochemical oxidation process of β -O-4 bond, a series of methoxyphenyl type β -O-4 dimer model compounds with different localized methoxyl groups, including 2-(2-methoxyphenoxy)-1-phenylethanone, 2-(2-methoxyphenoxy)-1-phenylethanol, 2-(2-methoxyphenoxy)-1-(4-methoxyphenyl)ethanone, 2-(2-methoxyphenoxy)-1-(4-methoxyphenyl)ethanol, 2-(2,6-dimethoxyphenoxy)-1-(4-methoxyphenyl)ethanone, 2-(2,6-dimethoxyphenoxy)-1-(4-methoxyphenyl)ethanol have been selected and their electrochemical properties have been studied experimentally by cyclic voltammetry, and FT-IR spectroelectrochemistry. Combining with electrolysis products distribution analysis and density functional theory calculations, oxidation mechanisms of all six model dimers have been explored. In particular, a total effect from substituents of both para -methoxy (on the aryl ring closing to C α ) and C α -OH on the oxidation mechanisms has been clearly observed, showing a significant selectivity on the C α -C β bond cleavage induced by electrochemical oxidations.
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2022
·
IF 6.556
Wenjing Gao,Bin Li,Ling Ling,Li Zhang,Shaoning Yu
Differentiating methicillin-sensitive and methicillin-resistant Staphylococcus aureus (MRSA and MSSA) is crucial for clinical diagnosis and anti-microbial treatment. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is an efficient tool for identifying pathogenic microorganisms at the bacterial species level. Here, we found that MRSA and MSSA can be differentiated by MALDI-TOF MS by employing (E)-propylα-cyano-4-hydroxyl cinnamylate (CHCA-C3) as the matrix, which shows great performance for proteins/peptides, especially hydrophobic proteins. The results show that the mass spectra profile of standard MRSA (ATCC 43300) is significantly different from the profiles of standard MSSA strains (ATCC 25923 and 29213) when using CHCA-C3 as the matrix compared to traditional matrix. The mass profiles had great reproducibility and were scarcely influenced by the growth medium. Due to the enhanced discrimination ability of CHCA-C3, we collected the mass spectra of 62 clinical S. aureus strains and selected four representative peaks for principal component analysis, which showed great differentiation. Our results suggest that employing a suitable matrix could enhance the discrimination ability of antibiotic-resistant bacteria by MALDI-TOF MS.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
·
2022
·
IF 5.195
Ningbo Cai,Bingtian Xu,Xing Li,Yunyun Qin,Mengfan Li,Kechun Chen,Jiangping Xu,Haitao Wang
The loss of tight junction (TJ) and adherens junction (AJ) proteins leads to the damage of the blood-brain barrier (BBB) during cerebral ischemia . Inhibition of cyclic nucleotide phosphodiesterase 4 (PDE4) by roflumilast (Roflu) protects against ischemic stroke-induced neuronal damage. However, the effects of Roflu on vascular endothelial injury and BBB integrity remain unknown. Here, we investigated whether and how Roflu protects against cerebrovascular endothelial injury caused by cerebral ischemia/reperfusion. We demonstrated that PDE4B knocking-down increased the expression of TJ and AJ proteins in human brain microvascular endothelial cells (HBMECs) subjected to oxygen-glucose deprivation reperfusion (OGD/R). Inhibition of PDE4 by Roflu (1.0 μM) showed similar effects as PDE4B knocking-down. We then found that Roflu activated Notch1/Hairy and enhancer of split 1 (Hes1) signaling. Consistently, the effects of Roflu on TJ and AJ proteins were reversed by the γ-secretase inhibitor DAPT or Hes1 knocking-down. Furthermore, Roflu (1.0 mg/kg) improved neurobehavioral outcomes and ameliorated BBB disruption in rats following ischemic stroke. Roflu also increased the levels of TJ proteins and AJ proteins in vivo . Collectively, these data suggest that Roflu is a promising compound for the prevention of BBB damage. The protective effects of Roflu are mediated through activation of the Notch1/Hes1 pathway.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2022
·Vol. 33
·期 8
·2620-2631
·
IF 3.348
Yuandou Wang,Shuxin Wang,Wenju Hu,Feng Su,Fusheng Liu,Suming Li
Amphiphilic block copolymers were synthesized by ring-opening polymerization of ε-caprolactone (ε-CL) using 4-arm poly(ethylene glycol) (4aPEG) as macroinitiator. 4aPEG and 4aPEG-PCL copolymers were end functionalized by introducing acrylic groups, and characterized using NMR, GPC, FT-IR and DSC analyses. Photo-crosslinked hydrogels were prepared by irradiation of acrylated polymer solutions under visible light (405 nm), using lithium phenyl-2,4,6-trimethylbenzoy-lphosphinate (LAP) as a photoinitiator. In situ gelation was achieved by subcutaneous injection of LAP containing copolymer solution into the abdomen of mice, followed by exposure to visible light. The hydrogels exhibit highly porous structure, high swelling up to 3300%, and excellent biocompatibility. The swelling ratio decreases with increase of poly(ɛ-caprolactone) (PCL) block length and polymer concentration of hydrogels. Doxorubicin hydrochloride (DOX·HCl) was loaded in hydrogels by soaking dried gel in a DOX solution. The drug loading efficiency is dependent on the swelling performance, reaching a maximum of 94.3%. In vitro drug release studies showed a burst release for the first 6 h, followed by a slower release up to 82% at 28 days. The release rate decreased with increase of hydrophobic block length or polymer concentration of hydrogels. Higher anti-cancer activity on A549 lung cancer cells was obtained for hydrogels with higher drug concentration, and shorter PCL block length. Therefore, 4aPEG-PCL hydrogels with outstanding biocompatibility, in situ gelation and prolonged drug release could be very promising for long-term local cancer therapy.
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Environmental Research and Public Health
·
2022
·Vol. 19
·期 5
·2934
·
IF 4.614
Hong Liu,Houwang Chen,Ning Ding
In this research, the preparation of a Ag3PO4/g-C3N4@MoS2photocatalyst and the performance and mechanism of degradation of 2-amino-4-acetaminoanisole (AMA) were studied. The phase composition and morphology of the synthesized samples were comprehensively characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), ultraviolet–visible diffuse reflectance (UV–Vis), and photoelectron spectroscopy (XPS). The catalytic performance of the photocatalyst was evaluated by the visible-light catalytic degradation of the AMA. The experimental results show that the Ag3PO4/g-C3N4@MoS2composite photocatalyst has stronger photocatalytic oxidation and reduction capabilities than Ag3PO4and Ag3PO4/g-C3N4. The material only decreases by 31.3% after five cycles of use, indicating that the material has good light stability. Free radical capture experiments prove that photo-generated holes (h+) and superoxide radicals (·O2−) are the main active substances in the photocatalytic process. The fundamental studies in the present research provide a new perspective for constructing an innovative type of visible-light photocatalyst and a new way to promote the photocatalytic degradation of organic pollutants.Keywords:catalysis;degradation;nanomaterials;Ag3PO4;heterojunction formation
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Yuenan Pan,Qi Chu,Shuang Guo,Sila Jin,Eungyeong Park,Yeonju Park,Yantao Sun,Lei Chen,Young Mee Jung
The purpose of this paper is to study the charge transfer (CT) process of a Ag/MBA/PAN system with different thicknesses of PAN and explore its SERS enhancement mechanism. It is found that as the PAN thickness is increased, the frequency and intensity of the characteristic MBA band gradually increases. The intensity of the b 2 mode increases significantly, which is attributed to the CT mechanism. As the PAN thickness is increased, the degree of CT (ρ CT ) gradually increases, which is due to the excited SPR from Ag. The electromagnetic enhancement mechanism, which occurs at the interface between PAN and Ag, generates hot electrons and rearranges the interface between PAN and Ag, thereby promoting electron transfer for the whole CT in Ag/MBA/PAN. These results show that CT occurs from the Fermi level of Ag to the LUMO level of MBA and then to the LUMO level of PAN. Additionally, the direction of CT is verified by 2D-COS. Therefore, this study not only reveals the ultrahigh SERS activity of the substrate but also explains the detailed process of regulating the substrate through the PAN thickness, which affects the SERS mechanism. This conductive polymer will open up a new horizon of SERS research and applications.
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 10
·1023-1030
·
IF 3.532
Hanxing Yang,Zhongguang Ji,Yanbo Zeng,Jian Zhang,Lifen Chen,Hailong Wang,Yiwen Yang,Longhua Guo,Lei Li
An aggregation-induced emission monomer-based fluorescent molecularly imprinted poly(ionic liquid) (AIE-FMIPIL) was synthesized for the first time with an AIE probe 4-(1,2,2-triphenylvinyl)phenyl acrylate (TPE), and an ionic liquid as dual functional monomers, and an ionic liquid as cross-linker. AIE-FMIPIL displayed a sphere-like shape and its average diameter was 410 nm. The absolute quantum yields of TPE and AIE-FMIPIL were 9.23% and 12.61%, respectively. The synergetic effect of TPE in the AIE-FMIPIL framework contributed to the higher quantum yield of AIE-FMIPIL. 4-Nitrophenol (4-NP) efficiently quenched AIE-FMIPIL with high fluorescence based on the Förster resonance energy transfer mechanism. The synthesized AIE-FMIPIL sensor was highly sensitive for 4-NP detection (linear range, 0.02–1.5 μM) in the optimal detection condition, with a low detection limit of 10 nM (S/N = 3). AIE-FMIPIL showed increased sensitivity and quenching efficiency compared with AIE-FMIP comprising a traditional monomer and cross-linker. AIE-FMIPIL exhibited selective binding to 4-NP because of the imprinted sites. AIE-FMIPIL was adopted to detect 4-NP in environmental samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Yu-Shi Shen,Xiao-Hui Yao,Chen-Xi He,Run-Ze Hu,Jia-Xin Yang,Dong-Yang Zhang,Tao Chen
Modern bioreactors primarily focus on fixing enzymes on materials to form fixed bed reactors, but the reaction efficiency is unstable. More importantly, the enzymes easily fall off or even inactivate, and the fluid risks leaking due to excessive pressure during flow. In this study, a directional channel continuous microfluidic reactor with laccase covalently immobilized in the internal channel is made from natural wood. After dimethylacetamide/lithium chloride (DMAc/LiCl) treatment of delignified wood, the micropores (ray cells, pits and nanopores) in directional channel walls are fully exposed. When the fluid passes through, the turbulence near the pits increases, resulting in good mass and heat transfer, which enhances the catalytic efficiency of laccase . The reactor has good stability and maintains more than 80% substrate conversion in the pH range of 3–7 and the temperature range of 15–55 ℃. In the degradation of 4-nitrophenol (4-NP), a 94.42% degradation rate is achieved in only 30 min, and 86.93% efficiency is maintained after 25 cycles of catalysis, indicating its excellent reusability. The directional reactor with porous inner walls is simple to prepare and easy to scale up, which provides excellent convenience for continuous industrial production and shows great commercial application potential.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）