SENSORS
·
2024
·Vol. 24
·期 4
·1093
·
IF 3.4
Zhen Li,Yang Hu,Liu Wang,Houfang Liu,Tianling Ren,Cong Wang,Daoliang Li
A surface-enhanced Raman scattering (SERS) method for measuring nitrate nitrogen in aquaculture water was developed using a substrate of β-cyclodextrin-modified gold nanoparticles (SH-β-CD@AuNPs). Addressing the issues of low sensitivity, narrow linear range, and relatively poor selectivity of single metal nanoparticles in the SERS detection of nitrate nitrogen, we combined metal nanoparticles with cyclodextrin supramolecular compounds to prepare a AuNPs substrate enveloped by cyclodextrin, which exhibits ultra-high selectivity and Raman activity. Subsequently, vanadium(III) chloride was used to convert nitrate ions into nitrite ions. The adsorption mechanism between the reaction product benzotriazole (BTAH) of o-phenylenediamine (OPD) and nitrite ions on the SH-β-CD@AuNPs substrate was studied through SERS, achieving the simultaneous detection of nitrate nitrogen and nitrite nitrogen. The experimental results show that BTAH exhibits distinct SERS characteristic peaks at 1168, 1240, 1375, and 1600 cm−1, with the lowest detection limits of 3.33 × 10−2, 5.84 × 10−2, 2.40 × 10−2, and 1.05 × 10−2μmol/L, respectively, and a linear range of 0.1–30.0 μmol/L. The proposed method provides an effective tool for the selective and accurate online detection of nitrite and nitrate nitrogen in aquaculture water.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2025
·
IF 3.9
Yiwen Gui,Ao Chen,Yiting Liu,Ning Cai,Jumei Li
A sensitive and stable substrate is crucial in Raman spectroscopic techniques for the effective detection of environmental contaminants. In this study, a novel core–shell composite, Ag@UiO-66, was developed by coating Ag nanospheres with a porous metal–organic framework (MOF), UiO-66-NH2. This composite combines high stability with selective enrichment capabilities for Cr(VI). Leveraging the unique properties of UiO-66-NH2, such as its porosity, abundant amine groups, and Zr nodes, Ag@UiO-66 effectively enriches Cr(VI) through pore-filling, electrostatic interactions, and Zr–O coordination. Direct analysis of water samples spiked with Cr(VI) showed detection limits of 6.53 × 10–8 M in pond water and 7.52 × 10–8 M in lake water. The composite also exhibited excellent signal uniformity and reproducibility, with a low relative standard deviation of 6.78% across 30 batches. Additionally, it demonstrated outstanding recyclability under simulated sunlight irradiation due to the solar photocatalytic properties of UiO-66-NH2. Ag@UiO-66 also showed superior stability compared to pristine Ag nanospheres when exposed to strong acids, bases, or oxidative environments (e.g., 1% H2O2 solution). Overall, Ag@UiO-66 shows great potential as a reliable substrate for the trace detection of chemical analytes via SERS.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Tianxing Fang,Qingyi Wei,Erwen Wu,Hongbin Pu
Malachite green (MG), with teratogenic and reproductive toxicity, is widely misused as an antimicrobial agent, posing a serious threat to animal and human health. Therefore, this study developed a novel recyclable Surface-enhanced Raman scattering (SERS) hydrogel substrate, TiO 2 /MXene/PAA-PAM@AuNPs (TMPA), by forming a dual hydrogen-bond network using a PAA-PAM hydrogel matrix with TiO 2 /MXene and embedding AuNPs, for detecting MG residues in aquatic products. This hydrogel exhibits high sensitivity, with a detection limit for MG of 8.13 × 10 −6 mg/L. Additionally, it can completely degrade surface-adsorbed MG within 60 min under light irradiation, achieving self-cleaning and enabling reuse for more than seven cycles, and can be stored for over 50 days while maintaining its SERS activity due to the support and preservation provided by the hydrogel network. Additionally, this substrate can rapidly detect MG residues on or within fish, offering a new strategy for monitoring MG residues in aquatic products.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Xin Zhao,Shibin Han,Cheng Chen,Xiaoyi Lv,Jiahe Li,Chen Chen
Celecoxib (CXB) is a non-steroidal anti-inflammatory drug used to prevent and treat arthritis. However, overdosing or improper use can cause adverse reactions. SERS is an important tool for therapeutic drug monitoring (TDM).The present study proposes an innovative detection scheme based on SERS deep learning, aiming to achieve qualitative identification and quantitative detection of CXB at the Raman spectrum level. In this paper, a composite SERS substrate consisting of silver (Ag)@ platinum (Pt)@ porous silicon Bragg mirror (PSB) was synthesized. Crystal violet (CV) was used as probe to characterize the substrate properties, and the substrate showed excellent SERS properties and long-term stability. The substrate significantly enhanced the SERS response capacity to CXB, showing excellent reproducibility and high sensitivity. The LOD and the recovery rate were both within the reasonable range when the substrate was used to detect CXB concentration in serum. Three deep learning models, GoogleNet, ResNet and VGG, were used to classify five kinds of blood drug concentrations. Among them, the GoogleNet model based on spectral dataset achieved the highest classification accuracy of 84.17 %. The deep learning-SERS integrated system supports blood drug concentration discrimination, helping doctors personalize treatment strategies for arthritis patients and improving clinical efficiency.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Zixun Wang,Dong Zhang,Yuxuan Wu,Xu Ye,Yayun Qian,Fengsong Chen
The low early diagnosis rate of hepatocellular carcinoma (HCC) severely impacts patient prognosis, making the development of highly sensitive and specific early diagnostic technologies crucial. MicroRNA-221 (miR-221), an aberrantly overexpressed biomarker in HCC, holds significant diagnostic potential. This paper constructed a capillary surface-enhanced Raman scattering (SERS) sensing platform utilizing CRISPR/Cas13a trans-cleavage and double-shell gold-silver nanorods (DS Au-AgNRs) to detect serum miR-221 in HCC patients. DS Au-AgNRs were synthesized and assembled onto aminated capillaries, followed by conjugation of Cy5-labeled single-stranded DNA (ssDNA) to the DS Au-AgNR surface via Au–S bonds. In the presence of miR-221, activated CRISPR/Cas13a trans-cleavage cleaves the ssDNA, releasing Cy5 from the sensor surface and diminishing the SERS signal, enabling miR-221 quantification. The synthesized DS Au-AgNRs exhibit uniform morphology and size, are uniformly distributed on the capillary, and form numerous “hotspots”, thereby significantly enhancing the SERS signal. According to the characteristic peak of Cy5 at 1074 cm−1, a linear relationship is established between the log concentration of miR-221 and the measured SERS intensity (y = −3527.97 × −35369.60, R2 = 0.97767), with a LOD as low as 4.17 × 10−17 M. The sensor demonstrated high specificity and high sensitivity, and its capacity to detect miR-221 expression aligned with qRT-PCR results when analyzing serum samples, confirming that hepatocellular carcinoma patients exhibited significantly higher miR-221 levels compared to healthy individuals. The capillary SERS sensor thus provides an accurate and convenient approach for early HCC diagnosis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Xingliang Cheng,Ying Chen,Gongke Li,Yuling Hu
Nanozymes, as catalytic nanomaterials mimicking natural enzymes, have emerged as powerful tools for analytical sensing. However, conventional methods for nanozyme synthesis and immobilization often suffer from time-consuming processes and inefficient substrate integration. Herein, we develop an innovative electrochemical strategy that achieves simultaneous synthesis and immobilization of sponge-like two-dimensional tetrakis(4-carboxyphenyl)porphyrin-Fe (Cu-TCPP(Fe)) nanozymes (termed as STCTFzymes) on Cu foils within only 6 min. Kinetic analysis confirms that the electrochemical growth follows a diffusion-controlled instantaneous nucleation mechanism. The resulting STCTFzymes exhibit enhanced operational stability enabled by firm substrate anchoring and facile recyclability through physical retrieval of the copper support. Leaching tests and post-recycling structural analysis further validate the excellent structural integrity and anti-leaching capability of STCTFzymes. Given the excellent peroxidase-like (POD) activity of STCTFzymes, a surface-enhanced Raman scattering (SERS) method based on aptamer-specific recognition was developed using ochratoxin A (OTA) as a model analyte. The OTA aptamer was adsorbed on the surface of STCTFzymes to block the catalytic site and turn off the POD-like activity. Upon specific binding of OTA to the aptamer, the enzyme activity of STCTFzymes was reactivated, resulting in a favorable change in SERS signal. The established method showed a wide linear range (0.3–500 ng/mL) and low detection limit (0.091 ng/mL) for OTA, with superior selectivity, high sensitivity and satisfactory stability. This electrochemically-assisted self-assembly provides a new approach for developing robust nanozymes architectures on heterogeneous substrates. When synergistically integrated with aptamer-based recognition, the STCTFzymes presents a universal strategy for sensitive detection of biotoxins exhibiting inherently weak Raman response.
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2024
·
IF 6.3
Tao Xu,Huan Liu,Jing Tang,Wenxi Wen,Chenjie Gu,Tao Jiang,Hongbin Xu
Surface enhanced Raman scattering (SERS) with high detection sensitivity has emerged as a fascinating protocol for monitoring trace amount of drugs in clinical serum sample. Herein, a simple and environmentally friendly photo-induced in-situ reduction strategy was proposed to synthesize RP@Ag composites with both SERS and photocatalytic activities. By carefully adjusting the concentration of the Ag source in the precursor solution, the amount and diameter of the decorated Ag nanoparticles were precisely tuned. The resulting optimal RP@Ag composites exhibited reliable SERS enhancement ability (enhancement factor: 1.76 × 10 7 ) and the highest degradation efficiency (degradation ratio: 95.04 %, irradiation time: 120 mins) in recyclable detection of Rhodamine B. After further confirming the reliable accuracy, sensitivity, homogeneity, and durability of the RP@Ag composites, they were used to monitor osimertinib and enabled an extremely low limit of detection as 1.63 × 10 −7 mg/mL. In particular, such a recyclable SERS platform achieved perfect recovery rates, the obtained deviations were all within 10 % in the monitoring of clinical samples from patients taking medicine for different durations.
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Hao-Cheng Hu,Shao-Hua Wu,Lei-Xin Jin,Jian-Jun Sun
In this work, a novel “turn-on” mode Au nanocubes (AuNCs) enhanced surface-enhanced Raman scattering (SERS) biosensing platform coupled with heated Au electrode (HAuE) and strand displacement amplification (SDA) strategy was proposed for highly sensitive detection of DNA adenine methylation (Dam) Methyltransferase (MTase) activity. The Dam MTase and Dpn I enzyme activities were significantly increased by elevating the HAuE surface temperature, resulting in the rapid production of template DNA for later SDA. During the SDA process, the released single-stranded DNA (ssDNA) could be amplified exponentially, and its concentration was positively related to the Dam MTase activity. The plasmonic AuNCs in SERS tags could provide significant SERS enhancement due to their “lightning rod” effect resulting from the sharp feature of the edges and corners of AuNCs. Because of these factors, the proposed biosensors exhibited high sensitivity in detecting the Dam MTase activity. The limit of detection was estimated to be 8.65 × 10 −5 U mL −1 , which was lower than that in most of the sensors for detection of Dam MTase activity in the literature. This SERS biosensor could also be used to screen inhibitors of Dam MTase and had the potential for detecting Dam MTase activity in real biological samples.
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Chen Chen,Ximo Wang,Yingfang Zhang,Xingying Li,Huiju Gao,Geoffrey I.N. Waterhouse,Xuguang Qiao,Zhixiang Xu
Herein, a molecularly imprinted surface-enhanced Raman spectroscopy (SERS) sensor was developed for the selective capture and sensitive detection of tryptamine in foods. The SERS sensor exploited silver nanoparticle-decorated TiO 2 (TiO 2 @Ag) substrates for Raman signal enhancement via synergistic effect of electromagnetic enhancement and photoinduced charge-transfer, whilst surface functionalization with the molecularly imprinted polymer ensured selective tryptamine capture. The SERS spectrum of tryptamine on the sensor closely matched that predicted by density functional simulations. The SERS intensity for tryptamine on the developed TiO 2 @ [email protected] sensor increased linearly with the logarithm of the tryptamine concentration over the range of 10 −6 –10 −2 mol L −1 , with a LOD of 4.85 × 10 −7 mol L −1 . Tryptamine was detected in a spiked white vinegar sample, and its recoveries were in the range of 92.00%–111.40%. The SERS sensor could be used for the detection of tryptamine in actual samples.
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Hao Lu,Yu Yang,Ruijue Chen,Wenyi Huang,Hao Cheng,Xueping Liu,Hongxing Kong,Lijun Li,Jun Feng
In this study, we synthesized gold nanoparticles (AuNPs) in situ on the surface of two-dimensional Cu-TCPP (meso-tetra(4-carboxyphenyl)porphine) metal– organic framework (MOF) nanosheets to produce AuNP@Cu-TCPP nanocomposites as surface-enhanced Raman scattering (SERS) substrates. The SERS signal was enhanced in two ways: aggregation of the AuNPs@Cu-TCPP near the SERS hotspot through the strong adsorption capacity of the MOF, and the synergistic effect between the charge-transfer enhancement of the Cu-TCPP MOF and the electromagnetic enhancement of plasmonic metal AuNPs. The SERS activity of AuNPs@Cu-TCPP was evaluated by the analysis of multiple model analytes; Rhodamine 6G (R6G) and benzidine were detected with detection limits below 10 –13 and 10 –12 mol·L –1 , respectively. We used AuNP@Cu-TCPP as a SERS substrate combined with an enzyme-linked immuno- sorbent assay (ELISA) to detect the tumor marker carboxylesterase 1 (hCE1) in human serum. The SERS signal of benzidine at 1609 cm −1 is linearly correlated with hCE1 in the concentration range of 0.1–120 ng·mL −1 . Owing to their high sensitivity and excellent SERS sensing capability, AuNPs@Cu-TCPP nanocomposites have potential applicability in the analysis of clinical samples.
✓ 已验证
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Lin Zhu,Xiaoyue Su,Hao Ma,Lianze Shan,Bing Zhao
Spectra in surface-enhanced Raman scattering (SERS) are always accompanied by a continuous emission known as “background”, which complicates the analysis with a poor signal-to-noise ratio (SNR), especially for qualitative and quantitative detection. Here, the SERS profile of the CH 3 NH 3 PbBr 3 substrate displays high similarity with the bulk Raman spectra, and its FWHM is exceeding narrow. The significantly increased SNR of the probe molecules indicates that the substrate helps to improve the quantitative analysis capability of the SERS technology and alleviate the background-related problems. For the CH 3 NH 3 PbBr 3 substrate, good linearity (R 2 = 0.9591) in the range of 1.0 × 10 -7 − 1.0 × 10 -3 mol/L was obtained and the limit of detection (LOD) is 7.88 × 10 -6 mol/L. The reason for this excellent performance is that the introduction of organic molecules splits the 3D CH 3 NH 3 PbBr 3 substrate to form a 2D perovskite that fixes the molecular orientation through chemical interactions. The CH 3 NH 3 PbBr 3 has the advantage of maintaining the conformation and orientation consistency of adsorbed molecules. Meanwhile, compared with the commonly used noble metal (Au, Ag) and semiconductor (ZnO, TiO 2 ) SERS substrates, the CH 3 NH 3 PbBr 3 substrate has obvious superiorities in both spectral peak intensity and peak shape. Furthermore, the CH 3 NH 3 PbBr 3 substrate possesses specific recognition ability that has the same enhancement effect for a series of pyridine-containing probe molecules. Finally, the enhancement mechanism is discussed according to the CT contribution. As a promising substrate for SERS technology, CH 3 NH 3 PbBr 3 will provide more possibilities for quantitative and multiplex detection in the future.
✓ 已验证
DOI
高影响因子
NANOTECHNOLOGY
·
2020
·Vol. 31
·期 41
·415301
·
IF 3.551
Qian Zhao,Guangqiang Liu,Hongwen Zhang,Weiping Cai
A simple and cost-effective route to fabricating gold-coated glass nano-stings' arrays is presented for highly efficient surface enhanced Raman spectroscopy (SERS) chips via reactive ion etching of the glass slide covered with polystyrene (PS) colloidal monolayer and sputtering deposition of gold on it. The as-fabricated SERS chips consist of the nearly-hemispherical particles with submicron-size, which are hexagonally arranged into the ordered arrays. There are many short and long Au-coated glass nano-stings standing vertically on the hemispherical particles, showing high density tips (up to 7.0 × 103 μm−2). The formation of such gold-coated glass hierarchically structured array is attributed to the geometry of PS colloidal monolayer and the nanoscale roughness of glass slide's surface. Further, it has been demonstrated that such gold-coated glass array chip is highly SERS-active and can be utilized to detect trace organophosphorous toxicant with the limit below ppt level and good reproducibility in measurements. The intensity of the Raman peak shows the linear relation, in logarithmic scale, with the organophosphorous concentration from ppt to ppm level, exhibiting the possibility of quantitative detection in a large concentration range. This work has demonstrated the cost-effective method to fabricate the high performance SERS chips for trace detection of toxic molecules.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）