CARBOHYDRATE POLYMERS
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2017
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IF 4.811
Taotao Xu,Junhua Li,Jun Cao,Wenxia Gao,Li Li,Bin He
Poly(pseudo)rotaxane (PPR) nanoparticles was facilely prepared using poly(ethylene glycol) (PEG) modified with protoporphyrin (PpIX) and α-cyclodextrin (α-CD) via host-guest interaction, the effect of α-CD number on the nanoparticle properties was investigated. The PEG with protoporphyrin (PEG-PpIX) end capping was synthesized via coupling reaction and the poly(pseudo)rotaxane nanoparticles with different amount of α-CD were fabricated by host-guest interaction between mPEG-PpIX and α-CDs. The final product was characterized by nuclear magnetic spectrum ( 1 H NMR), X-ray diffraction (XRD), atomic force microscope (AFM) and dynamic light scattering (DLS). The results showed that the poly(pseudo)rotaxane nanoparticles with uniform spherical shape was successfully prepared and doxorubicin (DOX) could be efficiently encapsulated in the nanoparticles. The amount of α-CDs in poly(pseudo)rotaxane nanoparticles was proportional to micellar size and drug release rate. The nanoparticles with higher α-CD number showed better anticancer efficacy in half maximal inhibitory concentration (IC 50 ) test. The cell internalization efficiency of DOX-loaded poly(pseudo)rotaxane nanoparticles could be further improved by lowering the α-CD number to receive smaller nanoparticle size.
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高影响因子
ACS Sustainable Chemistry & Engineering
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2017
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IF 5.951
Youping Zhou,Xijie Yin,Hubiao Yang,Jing Su,Huimin Yu,Yan Wang,Shuixiu Zhou,Saša Zavadlav
The chemical linkage between α-cellulose and lignin in plant cell walls has long been a controversial topic and crucial to devising effective strategies for sustainable biomass and bioenergy utilization. In this new contribution, we surveyed 80 Poaceae (Gramineae) species grown in tropical Hainan Island (China) to test the hypothesis that the presence of oxygen-containing chemical linkage in Poaceae species is widespread. Our innovative natural abundance oxygen isotopic analysis allowed us to infer that more than 1/3 of the species investigated has chemical (ether) linkages between α-cellulose and lignin in their leaf cell walls, with a species-specific 2–89 oxygen-containing bonds for every 1000 glucose units. However, the presence of such linkage appears to be phylogeny-dependent. On average, species of C3 photosynthetic mode are found to have more extensive oxygen-containing linkages than those of C4 photosynthetic mode. Our finding challenges the conventional view that no chemical bonds between α-cellulose and lignin are present in higher plant cell walls and calls for new strategies for further understanding of the chemical linkage between the two major constituents of cell walls. This is especially important in the context of renewed and growing interests in biomass, bioenergy, and plant cell wall structure studies.
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FOOD CHEMISTRY
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2025
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IF 9.8
Ling Li,Yanxin Huang,Qianqian Ding,Danli Wang,Tinglan Yuan,Gongshuai Song,Hyunbin Seong,Jinyan Gong
Milk protein and hyaluronic acid (HA) at different mass ratios were systemically characterized using the Maillard reaction. Combining FTIR and molecular docking results, both conjugates, α-casein-HA (α-CN-HA) and β-lactoglobulin-HA (β-Lg-HA), were prepared via hydrogen-bond interactions. Binding of HA promotes the structural folding of milk proteins and alters their secondary structures. The α-CN-HA and β-Lg-HA conjugates exhibited significantly improved solubility thermal and antioxidant activity. The functional properties of the conjugates were modulated by varying the ratio of milk proteins to HA. The α-CN:HA 1:2 ratio had the highest denaturation temperature (110.1 °C). For β-Lg-HA, the highest denaturation temperature (112.1 °C) was observed at a 1:4 ratio. Antioxidant capacity increased with decreasing HA content; the highest value was observed at a mass ratio of 3:1 for both conjugates. Our findings suggest that Maillard reaction improves the physiological and functional properties of milk proteins and HA conjugates.
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ANALYTICAL CHEMISTRY
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2024
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IF 6.7
Yaping Li,Yongqi Yang,Chunmeng Zhong,Dan Xiao,Cuisong Zhou
Sensitive detection of resistance mutation T790 M is of great significance for early diagnosis and prognostic monitoring of non-small-cell lung cancer (NSCLC). In this paper, we showed a highly sensitive detection strategy for T790 M using a three-level characteristic current signal pattern in an α-hemolysin nanopore. A probe was designed that formed a C–T mismatched base pair with wild-type/P and a T–T mismatched with the T790M/P. The T790M/P produced a unique three-level characteristic current signal in the presence of mercury ions(II): first, T790M–Hg2+–P entering the vestibule of α-HL under the transmembrane potential and overhang of probe occupying the β-barrel, then probe unzipping from the T790M/P, T790 M temporally residing inside the nanocavity due to the interaction with Hg(II), and finally T790 M passing through the β-barrel. The blocking current distribution was concentrated with a small relative standard deviation of about 3%, and the signal peaks of T790 M and wild-type can be completely separated with a high separation resolution of more than 2.5, which achieved the highly sensitive detection of T790 M down to 0.001 pM (confidence level P 95%) with a linear range from 0.001 pM to 1 nM in human serum samples. This highly sensitive recognition strategy enables the detection of low abundance T790 M and provides a method for prognostic monitoring in NSCLC patients.
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Nano Today
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2024
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IF 13.2
Hongbing Liu,Muse Ji,Yi Qin,Yin Sun,Hanxun Wang,Peifu Xiao,Jiansong Zhao,Yaxin Deng,Zhen Zhang,Jingxin Gou,Tian Yin,Haibing He,Guoliang Chen,Xing Tang,Yu Zhang
Cytokine storm and pulmonary fibrosis caused by Coronavirus Disease 2019 (COVID-19) are the two major culprits threatening human life and health. Herein, we engineered a novel disulfide-bridged carrier-free prodrug self-assembled nanoparticles (DSSL NPs) composed of Dexamethasone (Dex) and α-linolenic acid (LNA) for synergistic inhibition of cytokine storm and pulmonary fibrosis. DSSL NPs possess the capability to selectively target injured lung tissue and precisely release drugs after intravenous administration. Consequently, a single administration of DSSL NPs effectively mitigated pro-inflammatory cytokines and tissue damage in mice with lipopolysaccharide (LPS)-induced cytokine storm by blocking the TNF, NF-κB, and Toll-like receptor signaling pathways. Moreover, long-term administration of DSSL NPs alleviated the abnormal fibroblast activation and collagen deposition in mice with LPS-induced pulmonary fibrosis by inhibiting the Wnt-PCP signaling pathway without obvious Dex-related side effects. Together, DSSL NPs achieved the effect of ‘killing two birds with one stone’, presenting a safe and reliable strategy for comprehensive treatment for COVID-19-related diseases.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Na Li,Kaiyue Liu,Yue Zhang,Zhenzhen Hui,Ping Wang,Shuyang Sun,Chao Du
This study aimed to identify novel peptides with α-glucosidase inhibitory activity from Meretrix meretrix Linnaeus hydrolysates (MMLHs) following simulated gastrointestinal digestion (SGD) and to elucidate their inhibitory mechanisms. The molecular weight distribution of MMLHs progressively decreased during SGD, with hydrolysates from the intestinal digestion phase (MMLHs-I) exhibiting the strongest α-glucosidase inhibition (IC 50 : 0.14 ± 0.02 mg/mL). A total of 127 peptides were identified in MMLHs-I, among which 58 were unique compared to those from the gastric digestion phase. In silico screening and molecular docking identified six novel peptides—FAGDDAPR, VPLF, YRRL, WDH, PPLA, and WSG—with significant α-glucosidase inhibitory activity, displaying IC 50 values of 0.90, 1.30, 1.90, 1.20, 1.00, and 1.80 mg/mL, respectively. Among them, FAGDDAPR and PPLA exhibited the highest bioactivity. Kinetic analysis and fluorescence quenching assays demonstrated that both peptides acted as mixed-type inhibitors, forming stable complexes with α-glucosidase. Their inhibitory effects were primarily driven by hydrogen bonding and hydrophobic interactions, which hindered substrate binding and induced conformational changes in the enzyme. These findings suggest that peptides derived from MMLHs through gastrointestinal digestion possess potential antidiabetic properties, highlighting their promise as functional food ingredients for mitigating hyperglycemic damage.
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JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
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2024
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IF 5.8
Lingjiao Zhan,Chaozhong Wu,Fuqin Zhang,Juan Wang,Haotian Liu,Yutong Chen,Shuwei Yao,Yunzhu Ma,Wensheng Liu
The coordinated regulation for α-Al 2 O 3 and mullite structures in the alumina-mullite duplex fiber poses a challenging yet crucial task in achieving a fiber with exceptional high-temperature properties. In this study, Fe element was added into the alumina-mullite fibers in the form of iron sol and ferric nitrate solution, respectively, with the aim of obtaining a fiber consisting of fine mullite grains embedded with nano α-Al 2 O 3 grains. The results revealed that the addition of Fe element significantly enhances the crystallization process of mullite in alumina-mullite fibers. Specifically, adding iron sol resulted in the reduce of α-Al 2 O 3 formation temperature to 1300 °C, while introducing Ferric nitrate solution promoted the formation of mullite phase. Simultaneous introduction of 1.3 wt% iron sol and 1.3 wt% Ferric nitrate solution contributed to achieve alumina-mullite fiber with tensile strength of 2.06 GPa. In addition, the influence mechanism of Fe source on the phase transformation and microstructures of alumina-mullite fiber were discussed.
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SCIENCE OF THE TOTAL ENVIRONMENT
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2024
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IF 8.2
Xiaoping Liang,Jiehua Liang,Shengqi Zhang,Haowei Yan,Tiangang Luan
Di-2-ethylhexyl phthalate (DEHP), a widely utilized plasticizer , has been described as a potential obesogen based on in vivo disruption of hepatic lipid homeostasis and in vitro promotion of lipid accumulation. However, limited literature exists regarding the specific ramifications of DEHP exposure on obese individuals, and the precise mechanisms underlying the adverse effects of DEHP exposure remain unclear. This study aimed to assess the impact of DEHP on hepatic lipid metabolism in obese mice by comparing them to normal mice. Following a 10-week DEHP exposure period, the obese mice exhibited higher blood lipid levels, more severe hepatic steatosis, and more infiltrations of inflammatory cells in liver tissue than normal mice. Interestingly, the body weight of the mice exhibited no significant alteration. In addition, transcriptomic analyses revealed that both lipogenesis and fatty acid oxidation contributed to hepatic lipid metabolism dysregulation following DEHP exposure. More specifically, alterations in the transcription of genes associated with hepatic lipid metabolism were linked to the different responses to DEHP exposure observed in normal and obese mice. Additionally, the outcomes of in vitro experiments validated the in vivo findings and demonstrated that DEHP exposure could modify hepatic lipid metabolism in normal mice by activating the LXR/SREBP-1c signaling pathway to promote lipogenesis. At the same time, DEHP exposure led to inhibition of the Camkkβ/AMPK pathway to suppress β -fatty acid oxidation. Conversely, in obese mice, DEHP exposure was found to be associated with the stimulation of both lipogenesis and fatty acid oxidation via activation of the LXR/SREBP-1c and PPAR-α signaling pathways, respectively. The findings presented in this study first elucidate the contrasting mechanisms underlying DEHP-induced liver damage in obese and normal mice, thereby offering valuable insights into the pathogenesis of DEHP-induced liver damage in individuals with obesity.
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Acta Biomaterialia
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2016
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IF 6.008
Qianming Lin,Yumeng Yang,Qian Hu,Zhong Guo,Tao Liu,Jiake Xu,Jianping Wu,Thomas Brett Kirk,Dong Ma,Wei Xue
Hydrogels have attracted much attention in cancer therapy and tissue engineering due to their sustained gene delivery ability. To obtain an injectable and high-efficiency gene delivery hydrogel, methoxypolyethylene glycol (MPEG) was used to conjugate with the arginine-functionalized poly( l -lysine) dendron (PLLD-Arg) by click reaction, and then the synthesized MPEG-PLLD-Arg interacted with α-cyclodextrin (α-CD) to form the supramolecular hydrogel by the host-guest interaction. The gelation dynamics, hydrogel strength and shear viscosity could be modulated by α-CD content in the hydrogel. MPEG-PLLD-Arg was confirmed to bind and deliver gene effectively, and its gene transfection efficiency was significantly higher than PEI-25k under its optimized condition. After gelation, MMP-9 shRNA plasmid (pMMP-9) could be encapsulated into the hydrogel matrix in situ and be released from the hydrogels sustainedly, as the release rate was dependent on α-CD content. The released MPEG-PLLD-Arg/pMMP-9 complex still showed better transfection efficiency than PEI-25k and induced sustained tumor cell apoptosis. Also, in vivo assays indicated that this pMMP-9-loaded supramolecular hydrogel could result in the sustained tumor growth inhibition meanwhile showed good biocompatibility. As an injectable, sustained and high-efficiency gene delivery system , this supramolecular hydrogel is a promising candidate for long-term gene therapy. Statement of Significance To realize the sustained gene delivery for gene therapy, a supramolecular hydrogel with high-efficiency gene delivery ability was prepared through the host-guest interaction between α-cyclodextrin and PEGylated arginine-functionalized poly( l -lysine) dendron. The obtained hydrogel was injectable and biocompatible with adjustable physicochemical property. More importantly, the hydrogel showed the high-efficiency and sustained gene transfection to our used cells, better than PEI-25k. The supramolecular hydrogel resulted in the sustained tumor growth inhibition meanwhile keep good biocompatibility. As an injectable, sustained and high-efficiency gene delivery system, this supramolecular hydrogel is a promising candidate in long-term gene therapy and tissue engineering.
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Progress in Natural Science-Materials International
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2024
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IF 4.8
Mengxia Ma,Zhaoyang Li,Xu Yan,Yanli Mao,Chaohai Wang,Haiyan Kang,Qun Yan,Jieqiang Zhou,Zhongxian Song,Dandan Pang
Magnetic flower-like α-Fe 2 O 3 @N–C particles were synthesized by hydrothermal self-assembly method and oxygen-limited carbonisation in a glycerol and ethanol solution system using glucose as the carbon source, iron nitrate as the magnetic source and particle skeleton and urea as the nitrogen source. Four hydrothermal temperatures of 120 °C, 140 °C, 160 °C and 180 °C were used to modulate the adsorbent structure. Hydrothermal temperature of 140 °C, solution pH = 7, and a dosage of 0.06 g are the optimum conditions. The adsorption capacity of 2,4-dichlorophenol (2,4-DCP) onto MC-2 was determined as 108.556 mg g −1 at 25 °C. Experiments show that the adsorbents all fitted well to the Langmuir isothermal adsorption model and the quasi-secondary kinetic model, and the adsorption behaviour was dominated by monolayer chemisorption . The adsorption process was heat absorbing and spontaneous. Raman and XRD analysis showed that the adsorbent had a stable α-Fe 2 O 3 skeleton and carbon layers. FTIR and XPS analysis showed that the adsorbent had hydrogen bonding , electrostatic interaction and π-π bonding to 2,4-dichlorophenol.
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Food and Bioprocess Technology
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2024
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IF 5.3
Cao Zhaoxin,Su Jingyi,Luo Yali,Wang Xinyu,Liu Zhiming,Chen Yanqi,Zou Yijing,Shen Wen,Ge Xuemei
Carvacrol essential oil has broad-spectrum antibacterial properties, but the essential oil is volatile and unstable for long-term storage and use. In order to improve the relevant properties of carvacrol, in the present study, carvacrol was encapsulated with β-cyclodextrin (β-CD) using the saturated aqueous solution method, and response surface analysis was carried out, which gave a high encapsulation rate (63.58 ± 3.74%). The success encapsulation of carvacrol was confirmed by scanning electron microscopy (SEM), Fourier infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The release performance of the encapsulants was also tested at different temperature and humidity levels. In addition, polyethylene glycol (PEG) composite carvacrol-inclusive preservation paper prepared by these anhydrous conditions showed high antibacterial activity against Streptomyces fructus Monilinia fructicola , the main pathogen of peaches. Also, PEG was used to form the film in the anhydrous condition with the inclusion compound and ethylene inhibitor 1-methylcyclopropene (1-MCP) to obtain the film coated paper, and its effect on postharvest preservation quality of peach was studied. The results showed that PEG + Carvacrol-β-CD + 1-MCP-α-CD inclusion complexes (ICs) effectively prolonged the storage time and improved the fruit quality and enzyme activity of peach. This study provides a solution for the preparation of controlled-release coated paper with essential oils and 1-MCP bioactives to extend the shelf life of fruits and vegetables. Graphical Abstract
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APPLIED BIOCHEMISTRY AND MICROBIOLOGY
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2024
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IF 1
Zhu Y. B.,Zhang W.,Fan Y. L.,Chen Y. Y.,Zhu J. L.,Lu H. X.
Abstract Two immunochromatographic assays based on hematite (α-Fe 2 O 3 ) nanoparticles were developed for rapid detection of aflatoxin M1 (AFM1) and Escherichia coli O157:H7 in the milk. Hematite nanoparticles were prepared and utilized as colored signal labels. The competitive format was applied for AFM1 detection, while the sandwich format for E. coli O157:H7 determination. Under optimal conditions, the assay exhibited a visual detection limit of 0.10 ng/mL and quantitative determination limit of 0.017 ng/mL for AFM1. For E. coli O157:H7, a visual detection limit of 2.9 × 10 5 CFU/mL was achieved. The entire determination process could be accomplished within 15 min. Compared with traditional nanolabels, hematite nanoparticles showed advantages of facile preparation, high stability and easy bioconjugation. The developed assays provided effective and rapid screening strategies for AFM1 and E. coli O157:H7 in milk samples with high sensitivity and specificity.
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危险品化学品经营许可证（带存储）