Frontiers in Immunology
·
2022
·
IF 8.786
Tian Xiaoying,Fan Rongbo,He Hong,Cui Qingyu,Liang Xi,Liu Qiqi,Liu Tongjie,Lin Kai,Zhang Zhe,Yi Huaxi,Gong Piming,Zhang Lanwei
Food allergy is a serious public health problem because of its high incidence and risk. Probiotics can induce immune regulation in patients with allergic diseases, but its mechanism is not fully clear. In this paper, β-lactoglobulin (β-LG)-sensitized mice were used as models to explore the mechanism of Bifidobacterium animalis KV9 (KV9) and Lactobacillus vaginalis FN3 (FN3) on reducing allergic reactions and regulating immune cell function. The results showed that oral administration of KV9 and FN3 significantly reduced the scores of allergic symptoms, hypothermia symptoms, and serum levels of β-LG-specific immunoglobulins E (β-LG-sIgE), histamine, and mast cell protease in allergic mice. Flow cytometry analysis of intestinal dendritic cells (DCs) showed that the proportion of CD11c+major histocompatibility complex (MHC)-II+DCs, CD11c+CD80+DCs, and CD11c+ CD86+DCs increased after KV9 and FN3 intervention, indicating that the strains induced immature DCs and decreased the antigen-presenting capacity of DCs. Meanwhile, the toll-like receptor 4 (TLR4)-NF-κB signaling pathway was activated in DCs. The secretion of interleukin-12 (IL-12) was significantly increased, while interleukin-4 (IL-4) was decreased by DCs after KV9 and FN3 intervention, indicating that DCs have the potential to promote T-cell differentiation into T helper type 1 (Th1) cells. Furthermore, the proportion of CD3+CD8−IFN-γ+ T cells in the spleen increased, while CD3+CD8−IL-4+T cells decreased after oral administration of KV9 and FN3, correcting the T helper type 2 (Th2)-skewed immune responses. These results indicate that KV9 and FN3 reduce β-LG-induced allergic symptoms in mice, and suggest that the two potential probiotics might be used as an alternative therapeutic agent for mitigating food allergy.
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高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2023
·Vol. 34
·期 1
·1-14
·
IF 3.7
Tao Zhou-Shan,Li Tian-Lin,Wei Shan
Magnesium (Mg) and Selenium (Se) are essential elements for bone health and have been studied extensively for its powerful osteogenesis and promoting bone regeneration. The purpose was to observe whether Co-modified 3D-printed β-tricalcium phosphate with Mg and Se could promote bone defect regeneration in an ovariectomized(OVX) rat model. The MC3T3-E1 cells were co-cultured with the leachate of β-TCP, Mg-TCP, and Mg/Se-TCP and induced to osteogenesis, and the cell viability, ROS, and osteogenic activity were observed by Cell Count Kit-8(CCK-8), fluorescent probe 2′, 7′-dichlorofluorescin diacetate, Alkaline phosphatase (ALP) staining, Alizarin Red(RES) staining, western blotting(WB), and immunofluorescence. Then the β-TCP, Mg-TCP, and Mg/Se-TCP were implanted into the femoral epiphysis bone defect model of OVX rats for 12 weeks. Micro-CT and histology analysis were used to observe the therapeutic effect. In vitro results show that the cell mineralization and osteogenic activity of the Mg/Se-TCP group is significantly higher than the β-TCP group and Mg-TCP group. Protein expressions such as FOxO1, SIRT1, SOD2, Runx-2, Cola1a, and OC of the Mg/Se-TCP group are significantly higher than the Con group and the β-TCP group. The results of intracellular ROS and SIRT1 and SOD2 immunofluorescence showed that Mg/Se-TCP can restore the oxidative stress balance of osteoblasts. Micro-CT and histology analysis showed that treatment with Mg/Se-TCP showed the largest amount of bone tissue in the defect area ( p < 0.05), and exhibited lower values of residual biological material ( p < 0.05), compared to that of the β-TCP group and Mg-TCP group. Our research results confirm that Mg/Se-TCP can improve the activity and function of osteoblasts and enhance bone regeneration mediated by reducing intracellular ROS in OVX rat models. Graphical Abstract
The release of Mg and Se during the degradation of Mg/Se-TCP can improve the local bone repair ability. At the same time, it can also inhibit cell ROS, and ultimately greatly promote local bone repair.
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CHEMISTRY OF MATERIALS
·
2023
·
IF 8.6
Meijun Wan,Yunchao Zheng,Xuemei Dai,Honglin Yang,Jingqiu Zhou,Jing Ou,Yaxin Yang,Meifang Liao,Zhining Xia,Lujun Wang
Covalent organic framework (COF) as a kind of crystalline porous material possesses intriguing advantages, including low density, large surface area, permanent porosity, and excellent chemical stability. This novel material has shown great potential in the development of chromatographic stationary-phase materials. In this work, a room-temperature solvothermal method was utilized to yield a uniformly spherical COF-modified silica named Sil-COF. Then, the thiol-ene click chemistry was further used to modify thiolated β-cyclodextrin on the spherical Sil-COF composite for chiral separation called Sil-COF-CD. A series of characterization methods were performed to identify the successful construction of COF-modified stationary-phase materials. Then, three types of nonpolar substances realized baseline separation on the Sil-COF column. In addition, 2-phenylpropionic acid and 1-phenyl-1-propanol were selected as model enantiomers to identify the chiral recognition capability of the Sil-COF-CD column. Sil-COF and commercial β-CD columns were compared to further confirm the superiority of the prepared chiral column. In addition, both COF-modified columns possessed good repeatability. The results open a new avenue to apply spherical chiral COF for the preparation of the HPLC stationary phase.
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International Journal of Chemical Reactor Engineering
·
2022
·Vol. 21
·期 6
·753-765
·
IF 1.636
Jintao Tian,Hongyu Zhang,Xinyu Zhao,Wanyi Liu,Yasser Fakhri
Polyvinyl alcohol (PVA), acrylic acid (AA), and β-cyclodextrin (β-CD) were used as monomers, and ammonium persulfate was used as an initiator. Orthogonal tests were optimized the experimental condition, and aqueous polymerization was used to prepare poly-β-cyclodextrin/polyvinyl alcohol/polyacrylic acid (β-CD/PVA/PAA) hydrogel. The samples were characterized by FT-IR (Fourier transform infrared), SEM (Scanning electron microscopy), and XRD (X-ray diffraction). β-CD/PVA/PAA hydrogel was analyzed, which influenced external environmental factors on the β-CD/PVA/PAA hydrogel adsorption performance, and the kinetic behavior of β-CD/PVA/PAA hydrogel on ciprofloxacin (CIP) adsorption was explored. The results concluded that the prepared β-CD/PVA/PAA hydrogel has a well-defined three-dimensional network structure. The decrease in the pH of the CIP solution and the adsorption temperature reduces the adsorption reaction of β-CD/PVA/PAA hydrogel on CIP. The kinetics of CIP adsorption by β-CD/PVA/PAA hydrogel confirmed the pseudo-second-order kinetic model ( R 2 > 0.997), the maximum equilibrium adsorption amounts is 372.12 mg/g, the removal rate reaches 74.42%. The adsorption process was mainly chemisorption, the adsorption isotherm fits the Freundlich adsorption isotherm model ( R 2 > 0.946), and the adsorption process was heterogeneous with multi-molecular layer adsorption. The adsorption process inclined more toward the adsorption of inhomogeneous multi-molecular layers. The β-CD/PVA/PAA hydrogel retained 80% adsorption properties after three adsorption-desorption under optimal conditions.
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ONCOLOGY REPORTS
·
2023
·Vol. 49
·期 3
·1-14
·
IF 4.2
Sijia Han,Xueying Jin,Tianyu Hu,Feng Chi
Breast cancer remains the most common malignancy and the leading cause of cancer‑associated mortality in women worldwide. Lysosomal protein transmembrane 5 (LAPTM5), a lysosomal membrane protein, plays an important role in several human malignancies. However, the biological functions and mechanism of LAPTM5 in breast cancer remain unclear. In the present study, the potential tumor‑promoting effect of LAPTM5 was predicted by bioinformatics analysis. LAPTM5 was highly expressed in breast cancer clinical specimens. Moreover, in vitro studies demonstrated that cell proliferation, migration and invasion, as well as the process of epithelial‑mesenchymal transition (EMT) were promoted by LAPTM5 overexpression and were suppressed by LAPTM5 downregulation in vitro. The tumor‑promoting effects of LAPTM5 were also confirmed by xenograft tumor assay in vivo. It was found that the tumor‑promoting effects of LAPTM5 were partly dependent on the activation of the Wnt/β‑catenin signaling pathway. Furthermore, dual‑luciferase and chromatin immunoprecipitation assays verified that the transcription factor forkhead box protein 3 (FOXP3) directly bound to the promoter of LAPTM5 and negatively regulated its expression. Taken together, the present findings indicated that LAPTM5, negatively regulated by FOXP3, promoted the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin signaling pathway.
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高影响因子
APPLIED SURFACE SCIENCE
·
2023
·
IF 6.7
Jinyuan Zhang,Tong Liu,Tian Shi,Zhiyuan Cheng,Fuhua Gao,Da Cai,Shenghong Yang,Yueli zhang
Constructing comprehensive performance-enhanced heterostructured composite using the synergistic effect of complementary advantages between heterogeneous materials has always been challenging. In this work, highly stable β-NaYF 4 :Yb 3+ , Tm 3+ @BiOCl (NYF@BiOCl) heterostructured upconversion composites were successfully prepared using a two-step hydrothermal method. Compared to NYF/BiOCl mechanical mixtures, NYF@BiOCl with intimate interface show a higher photocatalytic degradation rate of rhodamine B (RhB) aqueous solutions, achieving 99.9% in 18 min and 98% in 2 h under simulated sunlight and near-infrared (NIR) light, respectively. The construction of heterostructures has a broad-spectrum response, which improves the utilization of sunlight and achieves efficient photocatalytic degradation of organic contaminants. Upconversion luminescence mechanism, spectral overlap, and Förster resonance energy transfer (FRET) explain the high photocatalytic activity of NYF@BiOCl. Meanwhile, NYF@BiOCl exhibits good universality to efficiently degrade other organic contaminants (methyl orange (MO), methylene blue (MB), bisphenol A (BPA), p-chlorophenol (4-CP)). This study demonstrates the potential of synergistic effects between heterogeneous materials. It Enhanced the photocatalytic activity of the NYF@BiOCl heterostructured composites driven by NIR light, providing a new strategy for energy-efficient and degradation of hazardous organic contaminants to alleviate environmental pollution.
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ACS Applied Nano Materials
·
2022
·
IF 6.14
Ruiqi Liu,Juan Yang,Rui Liu,Yumeng Tang,Lijin Huang,Qin Shuai
Covalent organic frameworks (COFs) featuring long-range ordered pore size and large specific surface area have been recognized as a fantastic platform for contaminant removal. However, the influence of nanopore size on their adsorption behavior has not been well understood. Herein, three stable β-ketoenamine COFs, i.e., TpPa-1, TpBD, and TpTph, with the same type of linkage but diverse nanopore size were successfully obtained by a salt-mediated crystallization strategy. Their adsorption behavior and interaction mechanisms toward sulfamerazine (SMR) in aqueous solution were studied by adsorption experiments and density functional theory (DFT) calculations. The adsorption kinetics of SMR over COFs followed the order TpPa-1 < TpBD < TpTph, which is consistent with the changing trend of nanopore size, suggesting that a large nanopore is beneficial in boosting mass transfer. However, the maximum adsorption capacities followed the order TpPa-1 (82.9 mg/g) < TpTph (150 mg/g) < TpBD (188 mg/g). Referring to the data of specific surface area and nanopore size of COFs, it can be inferred that the specific surface area of COFs plays a less important role, and pore characteristics might be the dominant factor affecting the adsorption behavior of SMR. Furthermore, DFT results confirmed that SMR can be loaded into the channels of TpPa-1, TpBD, and TpTph due to its small molecule size, and the adsorption interactions are dominated by C–H···π and H-bonding. The different adsorption performance of SMR on TpPa-1, TpBD, and TpTph stems from their different number of adsorption sites and binding energies. These findings demonstrated the feasibility of adjusting the adsorption capacity of COFs by regulating the nanopore sizes, providing possible solutions for tailor-made COFs with superior adsorption performance.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Yuying Xu,Minting Liang,Jintao Huang,Yapei Fan,Haiyue Long,Qunjie Chen,Zhe Ren,Chaoxi Wu,Yifei Wang
Single helical β -glucan is a one-dimensional host that can form a hybrid helix with DNAs/RNAs as delivery systems. However, unmodified β -glucan has a gelling tendency and a single helical conformation is challenging to obtain. Therefore, in this study, we developed a β -glucan formyl derivative with stable single helical conformation and no gelling tendency. Circular dichroism studies found that the formyl- β -glucan could form a hybrid helix with DNA CpG-poly(dA). The hybrid helix delivery system showed improved activation on antigen-presenting cells, thereby upregulating the mRNA and protein levels of inflammatory factors, and had an immune-enhancing effect on ovalbumin (OVA) immunized mice. These results indicate that formyl- β -glucan can be developed as a non-cationic supramolecular DNA delivery platform with low toxicity and high efficiency.
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Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 46
·9639-9653
·
IF 7.571
Xinyuan Yuan,Teliang Lu,Fupo He,Tingting Wu,Xiaolan Wang,Jiandong Ye
Biomaterials in combination with multiple bioactive ions could create a favorable microenvironment for bone remolding. Herein, zinc silicate/β-tricalcium phosphate (ZS/β-TCP) composite ceramic scaffolds with different amounts of ZS (5, 10, and 15 wt%) were constructed using a three-dimensional fiber deposition (3DF) technique. The physicochemical, osteogenic and angiogenic properties of these interconnected macroporous scaffolds were investigated systematically. Simultaneously, GeneChip, alkaline phosphatase (ALP), western blot (WB) and polymerase chain reaction (PCR) were utilized to elucidate the underlying mechanism of the enhancement in osteogenic differentiation. The results showed that the incorporation of ZS significantly improved the mechanical performance by more than 5 fold in comparison with the β-TCP ceramic scaffold (4.79 ± 0.99 MPa). The ZS modified β-TCP scaffolds greatly supported the cytoactivity, adhesion, proliferation of mouse bone marrow mesenchymal stem cells (mBMSCs) and human umbilical vein endothelial cells (HUVECs). The expression levels of osteogenic genes and proteins as well as angiogenic genes were markedly upregulated by the sustained release of bioactive ions (mainly Si and Zn) from the composite scaffolds. The 10ZS/β-TCP demonstrated the best overall performance in vitro. Moreover, the 10ZS/β-TCP displayed a high bone volume fraction, bone maturity and angiogenesis after implantation in the rat skull defects for 6 weeks. It was further verified that ZS/β-TCP scaffolds stimulated the osteogenic differentiation of mBMSCs by activating the p38 signaling pathway directly. The 10ZS/β-TCP ceramic scaffold holds great potential for the fast repair of bone defects, and deep understanding of the mechanism will facilitate the formulation of new strategies for bone repair.
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Journal of Water Process Engineering
·
2022
·
IF 7.34
Lingjie Ke,Jing Liu,Lei Sun,Fei Pan,Xiangjuan Yuan,Dongsheng Xia
In this paper, a series of copper oxide supported manganese dioxide (Cu X Mn T ) composites were prepared by the hydrothermal and deposition precipitation methods. The effects of different hydrothermal temperatures (100 °C–180 °C) and copper doping amounts (1–10 mM) on the structural and physicochemical properties of Cu X Mn T composites were systematically investigated. The BET, SEM , and XRD characterizations revealed that the Cu X Mn T composites exhibited a typical rod-like structure. Besides, with the increscent hydrothermal temperature, the BET specific surface area decreased and the crystal form of the composites changed from α-MnO 2 to β-MnO 2 . The Cu 1 Mn 180 with low specific surface area (3.277 m 2 g −1 ) exhibited the splendid catalytic ozonation activity and the oxalic acid (OA) removal rate reached 87.5% in O 3 /Cu 1 Mn 180 process compared with single ozonation (3.2%). Additionally, it was noteworthy that the excellent catalytic performance of Cu 1 Mn 180 (91.8% of OA removal rate) at pH 3.0 was resulted from the electrostatic adsorption. While, the complexation played a vital role for OA degradation at pH 6.0 and 9.0 with the OA removal efficiencies of 87.5 and 70.3% within 30 min, respectively. Furthermore, Cu 1 Mn 180 could obtain more than 90.9% of OA removal rate during multiple consecutive cycles, representing the satisfactory stability and reusability. Moreover, the multivalent Mn, the structural oxygen species, and Cu species played the key roles for OA degradation in O 3 /Cu 1 Mn 180 process at pH 6.0 via the TEM, ESR, XPS and O 2 -TPD characterizations. Overall, the results presented a promising alternative material for efficient catalytic ozonation application in water.
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Journal of Environmental Chemical Engineering
·
2022
·
IF 7.968
Haiyang Fan,Guiyun Yi,Zhengting Zhang,Xiuxiu Zhang,Peng Li,Chuanxiang Zhang,Lunjian Chen,Yulong Zhang,Qi Sun
Herein, we successfully fabricated the Ag deposited BiVO 4 (ABVO) electrode via the combination of electrodeposition and photodeposition for efficient photoelectrocatalytic degradation of β-naphthol under visible-light illumination (λ > 420 nm). The photocurrent density of ABVO photoanode was around 1.6 times higher than that of BiVO 4 (BVO) under visible-light irradiation at 1.5 V vs. Ag/AgCl, which was ascribed to the reduced recombination rate of photoinduced electron-hole pairs, as confirmed by electrochemical impedance spectroscopy (EIS) and Mott-Schottky plots. Additionally, the photoelectrocatalytic (PEC) degradation rate of β-naphthol (10 mg L −1 , 300 mL) by ABVO could approximately reach 80%, whose corresponding kinetic constant was 1.56 times higher than BVO. Based on the radical quenching experiments and EPR results, the •OH and •O 2 - radicals played crucial roles in PEC degradation of β-naphthol. The enhanced PEC performance of ABVO can be ascribed to the synergy of photocatalysis , electrocatalysis and the localized surface plasmon resonance (LSPR) effect of deposited Ag particles, which can effectively suppress the recombination of photoinduced electron-hole pairs, thereby prolonging the photo-excited charges lifespans and promoting the PEC performance. Furthermore, the ABVO photoanode possesses excellent recyclability , outstanding stability and strong resistance to acid and alkali, which can be a promising candidate for PEC organic contaminants degradation.
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Catalysis Science & Technology
·
2022
·Vol. 12
·期 6
·1968-1977
·
IF 6.177
Yiwen Yang,Haifeng Qi,Zhanwei Xu,Z. Conrad Zhang
In this work, we identified the prevailing catalytically active species evolved from paired PdCl2–CuCl2 in 1-butyl-3-methylimidazolium chloride ([BMim]Cl) that displayed dramatic synergy between the two metal chlorides in catalyzing the hydrolysis of β-1,4-glycosidic bonds in cellulose. Ultraviolet-visible (UV-vis), far infrared (FIR), and electron paramagnetic resonance (EPR) spectroscopy provide clear evidence for the prominent evolutionary pathways to form the catalytically active species. It is observed that the strong synergy between [PdIICl4]2− and [CuIICl4]2− is initiated by the presence of a saccharide. In the absence of a saccharide, the spectroscopic features of [PdIICl4]2− and [CuIICl4]2− in their paired solution show little change with respect to those of individual metal chloride solutions. The evolution of a highly active heterometallic PdII–Cl–CuI complex from paired PdCl2–CuCl2 in the presence of saccharides is revealed by the transition of the FIR absorption peak of PdII–Cl. Glycolaldehyde as the simplest model compound, which is the same as the reducing end group of saccharides, is applied to determine the possible catalytic species. X-ray absorption spectroscopy (XAS) verifies that [CuIICl4]2− is reduced to [CuICl3]2− by glycolaldehyde. The HCl generated from reduced [CuIICl4]2− is also found to contribute to the hydrolysis of cellobiose, serving as another catalytically active species in paired PdCl2–CuCl2/[BMim]Cl. Paired PdCl2–CuCl2 pretreated with glycolaldehyde exhibits dramatically higher activity than direct use of paired PdCl2–CuCl2 in cellobiose conversion. Strikingly, the PdII–Cl–CuI complex is found to be substantially more active than HCl in the hydrolysis of β-1,4-glycosidic bonds as evidenced by the sustained high cellobiose conversion after neutralization of HCl with NaOH.
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