Journal of Cardiovascular Translational Research
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2024
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IF 2.4
Hu Guangquan,Chen Jin,Chen Min,Yang Kai,Wang Yuchen,Ma Ziyang,Bao Huangxin,Ding Xiaojie
Cardiac fibrosis following myocardial infarction (MI) seriously affects the prognosis and survival rate of patients. This study aimed to determine the effect and regulation mechanism of the dedicator of cytokinesis 2 (DOCK2) during this process. Experiments were carried out in mice in vivo, and in Ang II treated cardiac fibroblasts (CFs) in vitro. DOCK2 was increased in mouse myocardial tissues after MI and Ang II-treated CFs. In MI mice, DOCK2 silencing improved cardiac function, and ameliorated cardiac fibrosis. DOCK2 knockdown suppressed the activation of CFs and decreased the expression of α-SMA, collagen I, and collagen III. Suppression of DOCK2 mitigated Ang II induced migration of CFs. DOCK2 inhibition reduced the activity of the PI3K/Akt and Wnt/β-catenin pathways, while this change could be reversed by the pathway activators, SC79 and SKL2001. In summary, DOCK2 suppression improves cardiac dysfunction and attenuates cardiac fibrosis after MI via attenuating PI3K/Akt and Wnt/β-catenin pathways.
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CATALYSIS LETTERS
·
2025
·Vol. 155
·期 3
·1-11
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IF 2.4
Wang Xiuqing,Yan Ruiyao,Fu Jianyu,He Yan,Zheng Jiahuan,Liu Liu,Zhou Na
The practical application of Cu 2 O in the field of photoelectrocatalytic (PEC) hydrogen production has been limited by its relatively low photoconversion efficiency and electron mobility. Plasmonic metal nanoparticles have been utilized to enhance the charge separation of semiconductors through resonance energy transfer from metal nanoparticles to semiconductors. In this study, Ag nanosphere (Ag NS)@SiO 2 were combined with Cu 2 O to form triple core–shell nanocomposites, aiming to enhance the photoelectrochemical activity of Cu 2 O under visible-light irradiation. The microstructures of the Ag@SiO 2 @Cu 2 O nanocomposites were regulated by controlling the thickness of SiO 2 interlay and Cu 2 O shell in order to optimize the PEC efficiency. It was found that Ag NS@SiO 2 (5 nm)@Cu 2 O (29 nm) NCs exhibited the highest photocurrent intensity, showing 3.3 times, 11.9 times, and 17.8 times higher values than pure Cu 2 O, pure Ag NS, and AgNS@SiO 2 NPs respectively. Furthermore, the photoelectrocatalytic hydrogen production velocity of Ag NS@SiO 2 (5 nm)@Cu 2 O (29 nm) NCs was around 25 mmol·g −1 ·h −1 , which has been improved around 4.2 times compared to pure Cu 2 O. This enhanced performance is attributed to plasmon-induced resonance energy transfer from Ag metal nanoparticles to Cu 2 O semiconductor, which may improve the separation efficiency of electron–hole pairs and lead higher photoelectrocatalytic efficiency. Graphic The Ag NS@SiO 2 was integrated with Cu 2 O to form triple core–shell nanocomposites, aiming to enhance the photoelectrochemical activity of Cu 2 O under visible-light irradiation through plasmon-induced resonance energy transfer from Ag to Cu 2 O. And their photoelectrocatalytic performances were optimized by controlling the thickness of SiO 2 interlay and Cu 2 O shell. Ag NS@SiO 2 (5 nm)@Cu 2 O (29 nm) NCs exhibited superior photocurrent intensity and enhanced photoelectrocatalytic hydrogen production rate compared to pure Cu 2 O.
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Nature Protocols
·
2024
·
IF 13.1
Zheng Jia-Jia,Zhu Feiyan,Song Ningning,Deng Fang,Chen Qi,Chen Chen,He Jiuyang,Gao Xingfa,Liang Minmin
Nanozymes are nanomaterials with enzyme-like catalytic properties. They are attractive reagents because they do not have the same limitations of natural enzymes (e.g., high cost, low stability and difficult storage). To test, optimize and compare nanozymes, it is important to establish fundamental principles and systematic standards to fully characterize their catalytic performance. Our 2018 protocol describes how to characterize the catalytic activity and kinetics of peroxidase nanozymes, the most widely used type of nanozyme. This approach was based on Michaelis–Menten enzyme kinetics and is now updated to take into account the unique physicochemical properties of nanomaterials that determine the catalytic kinetics of nanozymes. The updated procedure describes how to determine the number of active sites as well as other physicochemical properties such as surface area, shape and size. It also outlines how to calculate the hydroxyl adsorption energy from the crystal structure using the density functional theory method. The calculations now incorporate these measurements and computations to better characterize the catalytic kinetics of peroxidase nanozymes that have different shapes, sizes and compositions. This updated protocol better describes the catalytic performance of nanozymes and benefits the development of nanozyme research since further nanozyme development requires precise control of activity by engineering the electronic, geometric structure and atomic configuration of the catalytic sites of nanozymes. The characterization of the catalytic activity of peroxidase nanozymes and the evaluation of their kinetics can be performed in 4 h. The procedure is suitable for users with expertise in nano- and materials technology.
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Nature Nanotechnology
·
2024
·
IF 38.1
Wu Ziyu,Chen Lin,Guo Wenyan,Wang Jun,Ni Haiya,Liu Jianing,Jiang Wentao,Shen Jian,Mao Chun,Zhou Min,Wan Mimi
Mitochondrial transplantation is an important therapeutic strategy for restoring energy supply in patients with ischaemic heart disease (IHD); however, it is limited by the invasiveness of the transplantation method and loss of mitochondrial activity. Here we report successful mitochondrial transplantation by oral administration for IHD therapy. A nitric-oxide-releasing nanomotor is modified on the mitochondria surface to obtain nanomotorized mitochondria with chemotactic targeting ability towards damaged heart tissue due to nanomotor action. The nanomotorized mitochondria are packaged in enteric capsules to protect them from gastric acid erosion. After oral delivery the mitochondria are released in the intestine, where they are quickly absorbed by intestinal cells and secreted into the bloodstream, allowing delivery to the damaged heart tissue. The regulation of disease microenvironment by the nanomotorized mitochondria can not only achieve rapid uptake and high retention of mitochondria by damaged cardiomyocytes but also maintains high activity of the transplanted mitochondria. Furthermore, results from animal models of IHD indicate that the accumulated nanomotorized mitochondria in the damaged heart tissue can regulate cardiac metabolism at the transcriptional level, thus preventing IHD progression. This strategy has the potential to change the therapeutic strategy used to treat IHD.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-12
·
IF 14.7
Ji Qianqian,Tang Bing,Zhang Xilin,Wang Chao,Tan Hao,Zhao Jie,Liu Ruiqi,Sun Mei,Liu Hengjie,Jiang Chang,Zeng Jianrong,Cai Xingke,Yan Wensheng
The microscopic reaction pathway plays a crucial role in determining the electrochemical performance. However, artificially manipulating the reaction pathway still faces considerable challenges. In this study, we focus on the classical acidic water oxidation based on RuO 2 catalysts, which currently face the issues of low activity and poor stability. As a proof-of-concept, we propose a strategy to create local structural symmetry but oxidation-state asymmetric Mn 4-δ -O-Ru 4+δ active sites by introducing Mn atoms into RuO 2 host, thereby switching the reaction pathway from traditional adsorbate evolution mechanism to oxide path mechanism. Through advanced operando synchrotron spectroscopies and density functional theory calculations, we demonstrate the synergistic effect of dual-active metal sites in asymmetric Mn 4-δ -O-Ru 4+δ microstructure in optimizing the adsorption energy and rate-determining step barrier via an oxide path mechanism. This study highlights the importance of engineering reaction pathways and provides an alternative strategy for promoting acidic water oxidation.
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
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2024
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IF 3
Zou Yanyan,Li Yaoqiang,Zhang Jiaqing,Guo Yi,Song Tonglei,Li Kaiyuan,Xu Mingjun
The incineration of a large number of old cables leads to the problem of green recycling and causes a lot of environmental problems. This paper investigates the effect of copper oxide (CuO) capture of chlorine (Cl) in the pyrolysis recycling of polyvinyl chloride (PVC)-based waste cable outer sheath, focusing on the redistribution of the contaminant Cl components under the action of oxygen (O 2 ). Analytical methods such as ion chromatography and diffraction of X-rays are employed to measure and quantify the dynamic changes of the Cl-containing three-phase products during the pyrolysis process. The results indicate that, compared to copper (Cu), the oxidized state of CuO and O 2 -rich conditions can enhance the capture of Cl. With increasing O 2 supply, 1.71–6.33 mass% of gas-phase Cl and 14.52–29.96 mass% of liquid-phase Cl convert into 16.23–36.29 mass% of solid-phase Cl. CuO reacts directly with the PVC component of the outer sheath in a solid–solid reaction, significantly reducing the generation of polluting Cl-containing aromatic compounds in the liquid-phase tar and synergistically facilitating CaCO 3 components to intimately contact with the pyrolysis gas to capture HCl. The originally stable CuCl 2 crystals are oxidized again to enter into cyclic adsorption in the form of CuO under O 2 -rich conditions. Cl is more fixed in the residue and primarily exists in the form of stable and safe CuCl 2 and CaCl 2 metallic compounds. This study has clearly elucidated the efficient in situ green solidification mechanism of Cl during the treatment of cable waste with CuO in the presence of O 2 , providing new recommendations for cable sheath formulations and solid waste management.
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RARE METALS
·
2025
·
IF 11
Deng Shu-Yue,Zhou Xin-Yu,Zou Xiao-Peng,Tang Fang,Yang Dong,Sun Cai-Xia,Luo Jun,Ge Xing,Zhu Jia-Ying,Fang Tian-Ye,Yue Cai-Feng,Ju Yan-Min,Dai Jian-Jun
The presence of the blood–brain barrier limits the drug concentration in the brain, while low concentrations of antibiotics make it difficult to kill infecting bacteria and tends to induce drug resistance, making the clinical treatment of bacterial meningitis challenging. Herein, a nose-to-brain delivery strategy of small-sized nanozyme has been fabricated for combating bacterial meningitis, to overcome the low drug concentration and drug resistance. This strategy was achieved by a protein-supported Au nanozyme (ANZ). With a particle size of less than 10 nm, it possesses both glucose oxidase-like and peroxidase-like activities and can generate large amounts of reactive oxygen species through a cascade effect without the addition of external H 2 O 2 . Benefiting from the cascade catalytic amplification effect generated by its dual enzyme-like activities, ANZ shows significant broad-spectrum antibacterial activity without inducing bacterial resistance in vitro. Notably, small-sized ANZ exhibits higher brain entry efficiency and greater accumulation after intranasal administration compared to oral or intravenous administration. In a mouse model of bacterial meningitis, the mice treated with ANZ had lower bacterial loads in the brain and higher survival and clinical behavior scores compared to the classical antibiotic ceftriaxone. Additionally, the meningitis mice exhibited undamaged cognitive and behavioral abilities, indicating the excellent biocompatibility of ANZ. The above results demonstrate that nose-to-brain delivery of ANZ exhibits high intracerebral accumulation, strong antibacterial efficacy and does not lead to bacterial resistance. It holds broad prospects for the treatment of bacterial meningitis. Graphical abstract
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RARE METALS
·
2024
·
IF 9.6
Fu Mei-Qian,Han Yu-Yang,Nie Yan-Mei,Liu Yan-Di,Liu Yue,He Peng,Yu Wei-Dong,Li Xiang,He Piao,Li Juan,Yan Jun
Photothermal conversion is one of the key technologies in solar energy collection, seawater desalination, photothermal treatment and other important fields. In order to develop next generation photothermal materials, four polyoxometalates, [(CH 3 ) 2 NH 2 ] 12 H 5 [Ni 3 Mo 18 O 54 (HPO 3 ) 10 (PO 4 )]·18H 2 O (Compound 1), [(CH 3 ) 2 NH 2 ] 1 Na 11 [Ni 2 Mo 8 O 22 (HPO 3 ) 10 ]·16H 2 O (Compound 2), Na 15 (OH) 5 [Mo 6 O 18 (HPO 3 ) 4 ] 2 [MoO] 1.5 ·16H 2 O (Compound 3), [(CH 3 ) 2 NH 2 ] 4 Na 11 [Na[Mo 6 O 15 (HPO 3 ) 4 ] 2 ]·18H 2 O (Compound 4), are successfully designed and synthesized via a microwave-assisted reaction protocol. Compounds 1–4 not only exhibit broad absorption and notable photothermal conversion effects in near-infrared (NIR) region, but also have high photothermal conversion efficiencies and high quality NIR photothermal imaging effects under NIR laser irradiation. Compound 1 shows the best photothermal conversion effect, and it provides a unique model to explore the relationship between the complex metal oxide structure and photothermal conversion behavior at the molecular level. Both the experimental results and theoretical calculations consistently conclude that the charge and degree of electron delocalization on the Cluster have a robust influence on the photothermal conversion, as well as the aggregation microstructures. Graphical abstract
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-9
·
IF 14.7
Zhai Jianxin,Xia Zhanghui,Zhou Baowen,Wu Haihong,Xue Teng,Chen Xiao,Jiao Jiapeng,Jia Shuaiqiang,He Mingyuan,Han Buxing
Upcycling of CO 2 into fuels by virtually unlimited solar energy provides an ultimate solution for addressing the substantial challenges of energy crisis and climate change. In this work, we report an efficient nanostructured Ru/MnO x catalyst composed of well-defined Ru/MnO/Mn 3 O 4 for photo-thermal catalytic CO 2 hydrogenation to CH 4 , which is the result of a combination of external heating and irradiation. Remarkably, under relatively mild conditions of 200 °C, a considerable CH 4 production rate of 166.7 mmol g −1 h −1 was achieved with a superior selectivity of 99.5% at CO 2 conversion of 66.8%. The correlative spectroscopic and theoretical investigations suggest that the yield of CH 4 is enhanced by coordinating photon energy with thermal energy to reduce the activation energy of reaction and promote formation of key intermediate COOH* species over the catalyst. This work opens up a new strategy for CO 2 hydrogenation toward CH 4 .
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JOURNAL OF FLUORESCENCE
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2025
·
IF 3.1
Chen Xin,Wang Qing-wan,Zhan Tong-ling,Sun Yue,Zhang Xiao-rui,Liu Kun,Liu Xiao-wu
Nitrogen@Carbon quantum dots (N@CQDs) are prepared using microwave hydrothermal method, and polyvinylpyrrolidone (PVP) and melamine are used as mixed C source and N source. Microwave reaction conditions of preparing the N@CQDs are 170 ℃ and 3 h. This N@CQDs are are used as fluorescence probe for detection of amino acids. The N@CQDs can recognize L-alanine in water solution, and simultaneously recognize both L-methionine and L-cysteine in dimethyl sulfoxide (DMSO) solution. The linear ranges for L-alanine, L-methionine and L-cysteine are 0–15 µM, 2–50 µM and 0–17 µM, respectively. While limits of detection (LOD) for them are 11.092 nM, 7.8067 nM and 0.67476 nM, respectively. Compared to presently available detection methods, as-prepared N@CQDs have the advantage of high sensitivity and selectivity. Furthermore, coexistence of other amino acids has minimal interference in the detection of L-alanine, L-methionine and L-cysteine. For the as-prepared N@CQDs, solvent plays important roles in influencing the identification of amino acid types and detection sensitivity.
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Carbon Letters
·
2025
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IF 5.8
Ren Zhiyuan,Chang Wei,Tian Ke,Yao Peiqi,Liu Bin,Li Yunfeng,Zhang Runze
This study details the synthesis and characterization of phosphorus-sulfur co-doped graphitic carbon nitride quantum dots (PSQ) and their integration into g-C 3 N 4 (CN) to form PSQ/CN composites for the enhanced photocatalytic reduction of Cr(VI) and fluorescence detection. Incorporating PSQ into CN was found to significantly improve light absorption, narrow the band gap, and enhance charge separation efficiency. Notably, the composite material exhibits superior photocatalytic performance, especially in acidic environments. Photocatalytic assessments utilizing Cr(VI) demonstrated that the PSQ/CN composite outperformed both undoped and singly doped materials, indicating its superior photocatalytic activity. Additionally, phosphorus-sulfur co-doping markedly increased the fluorescence quantum yield of PSQ. The fluorescence intensity exhibited a linear decrease with increasing Cr(VI) concentrations, enabling sensitive and selective detection of Cr(VI) with a detection limit as low as 1.69 μmol/L. Collectively, the PSQ/CN composite and PSQ highlight their potential for photocatalysis and fluorescence-based detection of Cr(VI), providing high sensitivity, selectivity, and synergistic interactions within the composite material.
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Scientific Reports
·
2024
·Vol. 14
·期 1
·1-7
·
IF 3.8
Wang Xin,Gong Xianyun,Lin Binbin
Chlorogenic acid (CA) is an effective ingredient that can strengthen immunity during following the COVID-19 era. The current cost of CA is high owing to its complex purification process and low yield (approximately 2%). In this study, a one-step path orthogonal experiment was designed based on the results from Gauss calculation, which consisted of acidity, coordination, and hydrolysis in molecules. The optimized extraction conditions were 60 ℃, 60 min, 1:20 liquid ratio, and 40% ethanol in a nitrogen atmosphere controlled using a device of our own design, which led to CA yields of up to 6.35% from potato leaves. The purified CA was analyzed using high-performance liquid chromatography, thin-layer chromatography, ultraviolet–visible spectroscopy, and molecular fluorescence. This accurate and reproducible method can not only be used to obtain high yields of CA but can also be used for the quality control of active plant products and their isomers.
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