International Journal of Nanomedicine
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2023
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IF 8
Jin Zhang,Chuan-Zhen Ye,Ze-Yu Liu,Qian Yang,Yong Ye
Background Bacterial resistance to antibiotics is a persistent and intractable problem. The sapogenin isolated from the seeds of Camellia oleifera can inhibit antibiotic-resistant bacteria after structural modification.Purpose This study aims to improve sapogenin's antibacterial activity and avoid bacterial resistance based on nano-preparation with photo responsiveness.Methods The liposome shell material of carboxymethyl chitosan-phosphatidyl ethanolamine (CMC-PE) was prepared using amidation reaction, and photo-responsive cationic (PCC) liposomes containing Camellia sapogenin derivative (CSD) and photosensitizer pheophorbide-a were prepared by film dispersion method. Encapsulation efficiency, drug loading, zeta potential, particle size distribution, morphology and stability of the PCC liposomes were determined by HPLC, particle size analyzer, transmission electron microscopy (TEM) and fluorescence microscopy. Photo-responsive release of CSD in the PCC liposomes was determined by laser (0.5 mW/cm2) at 665 nm. Antibacterial activity of the PCC liposomes with or without irradiation was analyzed by MIC50, MBC, MBIC50, and bacterial morphology to evaluate the antibacterial effects on amoxicillin resistant Escherichia coli and Staphylococcus aureus.Results Size distribution, zeta potential, encapsulation efficiency and drug loading of the PCC liposomes were 189.23 ± 2.12 nm, 18.80 ± 1.57 mV, 83.52 ± 1.53% and 2.83 ± 0.05%, respectively. The PCC liposomes had higher storage stability and gastrointestinal stability, and no obvious hemolytic toxicity to rabbit red blood cells and no cytotoxicity after incubation with Hela cells. The photosensitizer pheophorbide-a was uniformly dispersed in the phospholipid layer of the PCC liposomes and increased the CSD release after irradiation. The PCC liposomes could bind to bacteria and impaired their morphology and structure, and had significant bactericidal effect on amoxicillin resistant E. coli and S. aureus.Conclusion The photo-responsive PCC liposomes are efficient antibacterial agents for avoidance of bacterial resistance against antibiotics.
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International Journal of Nanomedicine
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2023
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IF 8
Wei Xu,Honglan Wang,Lihui Dong,Pan Zhang,Yan Mu,Xueyan Cui,Jianping Zhou,Meirong Huo,Tingjie Yin
Introduction: Herein, a hyaluronic acid (HA)-coated redox-sensitive chitosan-based nanoparticle, HA(HECS-ss-OA)/GA, was successfully developed for tumor-specific intracellular rapid delivery of gambogic acid (GA).Materials and methods: The redox-sensitive polymer, HECS-ss-OA, was prepared through a well-controlled synthesis procedure with a satisfactory reproducibility and stable resulted surface properties of the assembled cationic micelles. GA was solubilized into the inner core of HECS-ss-OA micelles, while HA was employed to coat outside HECS-ss-OA/GA for CD44-mediated active targeting along with protection from cation-associated in vivo defects. The desirable redox-sensitivity of HA(HECS-ss-OA)/GA was demonstrated by morphology and particle size changes alongside in vitro drug release of nanoparticles in different simulated reducing environments.Results: The results of flow cytometry and confocal microscopy confirmed the HA-receptor mediated cellular uptake and burst drug release in highly reducing cytosol of HA(HECS-ss-OA)/GA. Consequently, HA(HECS-ss-OA)/GA showed the highest apoptosis induction and cytotoxicity over the non-sensitive (HA(HECS-cc-OA)/GA) and HA un-coated (HECS-ss-OA/GA) controls against A549 NSCLC model both in vitro and in vivo. Furthermore, a diminished systemic cytotoxicity was observed in HA(HECS-ss-OA)/GA treated mice compared with those treated by HA un-coated cationic ones and GA solution.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Yuan Xu,Zhicheng Deng,Yun Chen,Fang Fang Wu,Chao Huang,Yong Hu
A novel mussel-inspired adhesive hydrogel with enhanced adhesion based on methacrylated catechol-chitosan (MCCS) and dopamine methacrylate (DMA) was prepared via photopolymerization. The structure and morphology of the MCCS/DMA adhesive hydrogel were investigated by using FTIR , NMR, XRD, TG, and SEM. The rheological and texture properties, swelling and degradation characteristics, as well as the adhesion mechanism of the hydrogels were also examined. These results revealed that the MCCS/DMA hydrogels have a dense double cross-linking network structure with porous internal microstructures, and exhibited controllable swelling and degradation properties, good thermostability , and stable rheological characteristics. Furthermore, the adhesive mechanism of MCCS/DMA hydrogel has been confirmed by the FTIR and 2D correlation FTIR spectroscopy . Additionally, the results of in vitro cytotoxicity assessment indicated that the resulting hydrogels have good cytocompatibility. Overall, the MCCS/DMA adhesive hydrogel may have potential applications in medical bioadhesives.
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Membranes
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2022
·Vol. 13
·期 1
·36
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IF 4.562
Yunwu Yu,Chunyang Xie,Yan Wu,Peng Liu,Ye Wan,Xiaowei Sun,Lihua Wang,Yinan Zhang
In this study, a novel composite membrane was developed by casting the mixed aqueous solution of chitosan (CS) and polyvinyl alcohol (PVA) on a glass fiber microporous membrane. The polymeric coating of a composite membrane containing amino groups and hydroxyl groups has a favorable CO2affinity and provides an enhanced CO2transport mechanism, thereby improving the permeance and selectivity of CO2. A series of tests for the composite membranes were taken to characterize the chemical structure, morphology, strength, and gas separation properties. ATR-FTIR spectra showed that the chemical structure and functional group of the polymer coating had no obvious change after the heat treatment under 180 °C, while SEM results showed that the composite membranes had a dense surface. The gas permeance and selectivity of the composite membrane were tested using single gases. The results showed that the addition of chitosan can increase the CO2permeance which could reach 233 GPU. After a wetting treatment, the CO2permeance (454 GPU) and gas selectivity (17.71) were higher than that of dry membranes because moisture promotes the composite membrane transmission. After a heat treatment, the permeance of N2decreased more significantly than that of CO2, which led to an increase in CO2/N2selectivity (10.0).Keywords:carbon dioxide;polyvinyl alcohol;chitosan;glass fiber microporous membrane;gas separation
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INDUSTRIAL CROPS AND PRODUCTS
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2019
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IF 4.191
Liangfan Qu,Guiyun Chen,Shuang Dong,Yaoxing Huo,Zhonglin Yin,Shuhong Li,Ye Chen
Highly dispersible TiO 2 nanoparticles were prepared through the hydrolysis of titanium alcohol, and a series of TiO 2 -modified zein/chitosan (CS) films were prepared through a process involving blending, solution casting and evaporation. The mechanical properties and the antibacterial capacity of the zein/CS/TiO 2 films were characterized to explore the effects of different TiO 2 nanoparticle contents. Results showed that the addition of nanoscale TiO 2 remarkably improved the mechanical properties and thermal stability of the zein/CS/TiO 2 film. The tensile strength of the zein/CS/TiO 2 films significantly increased (p<0.05) with the increasing TiO 2 loadings in films, reaching a maximum of 28.33 ± 1.53 MPa at 0.15 wt % TiO 2 nanoparticles. The antibacterial effect of the composite films on Gram-positive bacteria ( S. aureus ) was more significant than that of Gram-negative bacteria ( E. coli and S. enteritidis ), and the antibacterial effect of UV light was better than that of dark conditions. In particular, the inhibition zones of zein/CS/TiO 2 (0.15%) film against E. coli , S. enteritidis and S. aureus under UV light condition increased by 21.78%, 21.45%, and 26.44%, respectively, compared with the control. This study suggested that adding highly dispersible TiO 2 nanoparticles is an effective method for improving the mechanical and antimicrobial properties of zein/CS films for various applications, such as medical and food packaging.
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Materials Science & Engineering C-Materials for Biological Applications
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2019
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IF 4.959
Yan Fang Cheng,Jie Yu Zhang,Yun Bing Wang,Chang Ming Li,Zhi Song Lu,Xue Feng Hu,Li Qun Xu
The titanium (Ti) and its alloys have been widely used for dental and orthopedic implants. However, the Ti-based implants may suffer from bacterial infection, which would result in insufficient healing, implant failure and repeated surgical intervention. It is of great interest to inhibit the bacterial adhesion and colonization on the Ti-based implants by introducing proper surface coatings. In this work, a simple method was employed to synthesize the water-soluble catechol-containing chitosan (CACS). The CACS coatings can be deposited onto various substrate surfaces and exhibit substrate-independent behavior. The CACS-coated Ti surfaces were further deposited with silver nanoparticles (Ag NPs) via in-situ reduction of Ag + ions using catechol moieties as the reducing agents. The resulting AgNPs/CACS-coated Ti surfaces exhibit antibacterial properties and can prevent the surface adhesion of bacterial cells, as evidenced by the inhibition zone test, live/dead bacterial staining assay and spread plate method. In addition, they show negligible cytotoxicity to L929 mouse fibroblast cells.
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Materials Science & Engineering C-Materials for Biological Applications
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2018
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IF 5.08
Ruixue Yin,Jing He,Meirong Bai,Cong Huang,Kemin Wang,Hongbo Zhang,Shih-Mo Yang,Wenjun Zhang
The closed-loop delivery of insulin in response to change of the blood glucose level and long-term supply of insulin are both important for diabetes patients. However, combination of these two goals in a chemically controlled implantable system is still challenging yet highly desirable. The purpose of the present study is to design a synthetic artificial pancreas by integration of chitosan hydrogels with insulin-loaded glucose-responsive microspheres to deliver insulin in a close-looped and long-term way for diabetes care. Glucose-responsive insulin-loaded microspheres were firstly fabricated via a high-speed shear-emulsion based crosslinking method and then embedded into chitosan hydrogels to make a scaffold-based synthetic artificial pancreas. In vitro experiments indicated the scaffold exhibited a longer insulin supply as well as a lower burst release compared with free microspheres, and could keep the glucose-responsive insulin release property inherited from the corresponding microspheres even after 12 day-release. The released insulin was proved to remain active, and the culture of HDF cells on the scaffold showed good cell proliferation during 7 days incubation. These results suggested the scaffold-based synthetic artificial pancreas have great promise in the application of insulin delivery.
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FOOD CHEMISTRY
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2018
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IF 4.946
Xiaofeng Ren,Ting Hou,Qiufang Liang,Xi Zhang,Di Hu,Baoguo Xu,Xinxiang Chen,Meram Chalamaiah,Haile Ma
In this study, resveratrol was successfully encapsulated using zein-chitosan complex coacervation. The encapsulation efficiency was markedly improved (51.4%) after chitosan coating at 1:2.5 zein/chitosan ratio, compared with 38.6% using native zein . Analysis of multi-model frequency ultrasound treatment effects on resveratrol encapsulation using zein-chitosan complex coacervation showed that 28/40 kHz dual-frequency ultrasound led to the highest encapsulation efficiency (65.2%; 31.9% increase) and loading capacity (5.9%; 31.1% increase) of resveratrol, followed by multi-frequency ultrasound at 20/28/40 kHz (17.8% encapsulation efficiency increase; 17.8% loading capacity increase). Dual-frequency ultrasound treatment significantly reduced the zein-chitosan complex coacervation particle size and reduced their distribution, however, did not change the zeta potential. Fourier transform infrared spectroscopy and fluorescence spectroscopy analysis demonstrated that ultrasound treatment had no effect on secondary structure of zein-chitosan complex but markedly decreased the fluorescence emission intensity. Differential scanning calorimetry and X-ray diffraction results indicated that Dual-frequency ultrasound treatment improved the thermal stability of zein-chitosan complex coacervation but had no effect on the crystal structure. Atomic force microscopy and scanning electron microscopy images revealed uniform distribution of zein-chitosan complex coacervation followed by ultrasonic treatment.
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MATERIALS & DESIGN
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2018
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IF 4.525
Shi-Neng Li,Baoqiang Li,Li-Xiu Gong,Zhi-Ran Yu,Yujie Feng,Dechang Jia,Yu Zhou,Long-Cheng Tang
Hyperbranched polysiloxane (HSi) molecules with tunable molecular structure were synthesized and served as a novel crosslinker for fabricating polyacrylamide/chitosan (PCH) hydrogels. Various characterizations demonstrated that HSi molecules with tunable bi-functional vinyl and epoxy groups were successful prepared and utilized to optimize the network architecture and interactions of polymer chains. Benefiting from the structure of bi-functional crosslinker, PCH hydrogels demonstrated remarkably improved mechanical properties, superior to that of conventional crosslinker or HSi with single vinyl or epoxy group. A tensile strength of 302 kPa, elongation at break of 2263%, and toughness of 3.85 MJ·m –3 can be achieved by optimizing the molecular structure and content of HSi with bi-functional group (e.g. ratio of vinyl/epoxy = 0.68). Additionally, such hydrogels showed outstanding antifatigue ability to withstand cyclic compressive tests. Based on the structure observation and analysis, the improved mechanical properties are likely derived from the combination of homogeneous and strong polymer network and improved interactions of polymer chains (e.g. covalent and hydrogen bonds). Approaches in this study may inspire the design and development of novel crosslinker for fabricating mechanically robust hydrogels.
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ACS Applied Bio Materials
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2018
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IF 0
Chunping Su,Huiping Zhao,Hao Yang,Rong Chen
In this work, we developed a facile approach for the fabrication of stearic acid-modified starch/chitosan composite sponge with asymmetric wettability and gradient wettability via mediating the amount of starch. The starch/chitosan composite sponge exhibited heterogeneous wetting properties on its two surfaces after stearic acid modification. The superhydrophobic top surface with a water contact angle of 150° could prevent water, blood, and bacterial permeation without losing the breathability of the sponge, whereas the superhydrophilic bottom surface possessed optimal water and blood absorption and clotting abilities. The asymmetric wettability of starch/chitosan composite sponge was attributed to the synergistic contribution of gradient distribution of starch, rough three-dimensional skeleton structure induced capillary effect and the nonuniform surface distribution of hydrophilic/hydrophobic groups. Furthermore, the sponge also possessed good biodegradability and noncytotoxicity to the human normal hepatocyte. The results indicated that the stearic acid-modified starch/chitosan composite sponge with ideal liquid absorption, vapor transmission rate, self-cleaning and stain-repellent ability could be used as a promising candidate for wound dressing.
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CARBOHYDRATE POLYMERS
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2018
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IF 5.158
Yongjun Sun,Wenquan Sun,Kinjal J. Shah,Pen-Chi Chiang,Huaili Zheng
In this work, carboxylated chitosan modified flocculant (CC-g-PCD) was prepared by graft copolymerization technique to enhance the charge-attracting and adhesion of bridges and net-sweeping capacity of flocculants. The dimethyldiallylammonium chloride (DMDAAC), carboxylated chitosan (CMCS), and 3-chloro-2-chloropropyltrimethylammonium chloride (CTA) were utilized for synthesis of CC-g-PCD via photopolymerization techniques. The synthesized CC-g-PCD was characterized by 1 H NMR, SEM, XRD, and FTIR, and the characteristic groups on the main chain and surface morphological structure of CC-g-PCD were investigated. The obtained results indicated that CTA and DMDAAC were successfully grafted into the CPCTS. In ordered to evaluate the flocculation performance of CC-g-PCD at various dosages, stirring intensity (G value), and pH value by detecting Chl a, COD, and turbidity, the actual lake water that contains algae was used for flocculation assessment tests. The experimental results of the water sample with flocculation showed that the maximum flocculation efficiency of turbidity (91.1%), Chl a (97.2%), and COD (97.0%) can be achieved by CC-g-PCD at pH 7, G value 200 s −1 , and 4.0 mg/L. The comparison results demonstrated that CC-g-PCD had better flocculation efficiency than commercial flocculants. Finally, based on the analysis of algae removal in combination with Zeta potential measurements, the flocculation mechanisms in actual lake water at various dosages and pH values were adsorption bridging and electrical neutralization.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
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IF 4.784
Songyan Wang,Yuqing Lu,Xiao-kun Ouyang,Xue Xue Liang,Di Yu,Li-Ye Yang,Fangfang Huang
Groundwater contaminated by arsenic endangers our health. Therefore, in this work, a novel composite adsorbent consisting of magnetic chitosan (MCS), zinc oxide (ZnO), and sodium alginate (Alg) was prepared to remove arsenic from groundwater. First, chitosan was coated on the surface of Fe 3 O 4 nanoparticles by coprecipitation. Then, MCS/ZnO@Alg gel beads were fabricated by combining MCS with ZnO and Alg , and crosslinking the composite material in the presence of Ca 2+ ions. The MCS/ZnO@Alg beads were characterized by SEM, FTIR spectroscopy , XRD, VSM, and XPS . The adsorption experiments revealed that the MCS/ZnO@Alg magnetic gel beads have high stability and As(V) adsorption capability, and adsorbed As(V) through chemical adsorption. The maximum As(V) adsorption capacity as determined from the Langmuir model was 63.69 mg/g. In addition, MCS/ZnO@Alg exhibited good recyclability and high sustainability. This work proves that the MCS/ZnO@Alg gel beads are an ideal candidate for addressing the grievous environmental threats caused by water pollution.
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