ANALYTICAL BIOCHEMISTRY
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2014
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IF 2.305
Hui Liu,Yong-Sheng Wang,Jia-Cheng Wang,Jin-Hua Xue,Bin Zhou,Hui Zhao,Shan-Du Liu,Xian Tang,Si-Han Chen,Ming-Hui Li,Jin-Xiu Cao
A highly sensitive, low-cost colorimetric aptasensor was developed for the determination of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in human urine. The method is based on a conformational switching of the 8-OHdG aptamer to form a G-quadruplex structure in the presence of 8-OHdG. The resulting G-quadruplex assembles into a peroxidase-like DNAzyme with hemin , which effectively catalyzes the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS 2− ) by H 2 O 2 to ABTS + , resulting in an increase in the absorption signal at 416 nm along with a color change of the solution. The response signals linearly correlated with the concentration of 8-OHdG, ranging from 466 pM to 247 nM with a detection limit of 141 pM. The relative standard deviation and the recovery were 1.97–3.47% ( n = 11) and 98.8–100.2%, respectively. The proposed method avoids the label and derivatization steps in common methods and allows direct analysis of the samples by the naked eye without costly instruments, which is reliable, inexpensive, and sensitive.
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APPLIED CATALYSIS A-GENERAL
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2015
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IF 3.942
Hong Liu,Zhengzheng Xu,Zhe Zhang,Dan Ao
A series of novel visible light-responsive Mn 0.8 Cd 0.2 S/g-C 3 N 4 hybrid materials with different g-C 3 N 4 contents were synthesized via a facile hydrothermal method. The obtained Mn 0.8 Cd 0.2 S/g-C 3 N 4 composites displayed highly efficient photocatalytic activities for H 2 evolution from aqueous solutions containing sacrificial reagents (Na 2 S and Na 2 SO 3 ) under visible light ( λ > 420 nm) even without noble metal co-catalysts. The highest H 2 evolution rate of 4.0 mmol h −1 g −1 (with an apparent quantum efficiency of 4.1% at 420 nm) was achieved on the Mn 0.8 Cd 0.2 S/g-C 3 N 4 (10 wt%) sample, which was about 3.40 times higher than that of pure Mn 0.8 Cd 0.2 S. The enhanced photocatalytic activity of Mn 0.8 Cd 0.2 S/g-C 3 N 4 composites should be attributed to the well-matched band structure and intimate contact interfaces between Mn 0.8 Cd 0.2 S and g-C 3 N 4 , which led to the effective transfer and separation of the photogenerated charge carriers. In addition, the Mn 0.8 Cd 0.2 S/g-C 3 N 4 photocatalysts showed good stability during the photocatalytic water splitting to hydrogen under visible light. A possible mechanism of the enhanced photocatalytic activity of Mn 0.8 Cd 0.2 S/g-C 3 N 4 was also proposed.
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BIOMACROMOLECULES
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2015
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IF 5.75
Piming Ma,Long Jiang,Pengwu Xu,Weifu Dong,Mingqing Chen,Piet J. Lemstra
In this work we report the in situ preparation of fully biobased stereocomplex poly(lactide) (SC-PLA) nanocomposites grafted onto nanocrystalline cellulose (NCC). The stereocomplexation rate by compounding high-molar-mass poly(d-lactide) (PDLA) with comb-like NCC grafted poly(l-lactide) is rather high in comparison with mixtures of PDLA and PLLA. The rapid stereocomplexation was evidenced by a high stereocomplexation temperature (Tc-sc = 145 °C) and a high SC crystallinity (Xc-sc = 38%) upon fast cooling (50 °C/min) from the melt (250 °C for 2 min), which are higher than currently reported values. Moreover, the half-life crystallization time (175–190 °C) of the SC-PLA was shortened by 84–92% in comparison with the PDLA/PLLA blends. The high(er) stereocomplexation rate and the melt stability of the SC in the nanocomposites were ascribed to the nucleation effect of the chemically bonded NCC and the “memory effect” of molecular pairs in the stereocomplex melt because of the confined freedom of the grafted PLLA chains.
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PHARMACEUTICAL RESEARCH
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2013
·Vol. 31
·期 2
·291-304
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IF 4.742
Yang Xiao-Li,Luo Yan-Ling,Xu Feng,Chen Ya-Shao
Purpose Block copolymer micelles are extensively used as drug controlled release carriers, showing promising application prospects. The comb or brush copolymers are especially of great interest, whose densely-grafted side chains may be important for tuning the physicochemical properties and conformation in selective solvents, even in vitro drug release. The purpose of this work was to synthesize novel block copolymer combs via atom transfer radical polymerization, to evaluate its physicochemical features in solution, to improve drug release behavior and to enhance the bioavailablity, and to decrease cytotoxicity. Methods The physicochemical properties of the copolymer micelles were examined by modulating the composition and the molecular weights of the building blocks. A dialysis method was used to load hydrophobic camptothecin (CPT), and the CPT release and stability were detected by UV–vis spectroscopy and high-performance liquid chromatography, and the cytotoxicity was evaluated by MTT assays. Results The copolymers could self-assemble into well-defined spherical core-shell micelle aggregates in aqueous solution, and showed thermo-induced micellization behavior, and the critical micelle concentration was 2.96–27.64 mg L −1 . The micelles were narrow-size-distribution, with hydrodynamic diameters about 128–193 nm, depending on the chain length of methoxy polyethylene glycol (mPEG) blocks and poly(N-isopropylacrylamide) (PNIPAM) graft chains or/and compositional ratios of mPEG to PNIPAM. The copolymer micelles could stably and effectively load CPT but avoid toxicity and side-effects, and exhibited thermo-dependent controlled and targeted drug release behavior. Conclusions The copolymer micelles were safe, stable and effective, and could potentially be employed as CPT controlled release carriers.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2015
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IF 2.979
Hui Liu,Yong-Sheng Wang,Xian Tang,Hui-Xian Yang,Si-Han Chen,Hui Zhao,Shan-Du Liu,Yu-Feng Zhu,Xiao-Feng Wang,Yan-Qin Huang
A sensitive, lable-free and low cost fluorescence aptasensor was developed for detecting 8-hydroxy-2′-deoxyguanosine (8-OHdG) by using 8-OHdG aptamer (Apt) as a recognition probe and N-methyl mesoporphyrin IX (NMM) as a reporter. The method is based on the conformational switching of a K + -stabilized G-quadruplex to a 8-OHdG-stabilized one. NMM can selectively bind to K + -stabilized G-quadruplex instead of 8-OHdG-stabilized one. The addition of 8-OHdG in the solution of Apt - K + ions leads to a sharp change in fluorescence intensity, which showed a good linear response toward 8-OHdG concentration ranging from 3.96 nM to 211 nM with a detection limit of 1.19 nM. The relative standard deviation and the recovery were 1.23–3.26% ( n = 11) and 94.8–106.7%, respectively. The proposed aptasensor consists of only an aptamer probe and a specific dye NMM, avoiding the complex and expensive labeling procedure. Thus it is much cheaper and more applicable.
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Materials Science & Engineering C-Materials for Biological Applications
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2017
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IF 4.164
Buguang Zhou,Min He,Ping Wang,Haitian Fu,Yuanyuan Yu,Qiang Wang,Xuerong Fan
Silk fibroin (SF) as a bioactive protein can offer growth substrates for hydroxyapatite (HAp) deposition. In the current work, graft copolymerization of acrylic acid (AA) onto fibroin chains was carried out using hydrogen peroxide-horseradish peroxidase (H 2 O 2 -HRP) catalytic system, SF-g-polyacrylic acid (PAA) membranes was prepared subsequently, followed by in situ biomimetic mineralization in the Ca/P solutions, aiming at promoting the deposition of HAp and endowing the fibroin-based biocomposite with enhanced bioactivity. Meanwhile, p -hydroxyphenylacetamide (PHAD) and methyl acrylate (MA), as the model compounds of tyrosine residues in SF and vinyl monomer were used to disclose the mechanism of graft copolymerization. The data from FTIR and SEC chromatograms indicated that vinyl monomer was successfully graft copolymerized with SF during H 2 O 2 -HRP treatment. According to the results of XRD, SEM patterns and EDS-Mapping, mineral phases on the surfaces of SF-g-PAA membranes were detected after different cycles of biomimetic mineralization, and the mechanical property of SF-g-PAA/HAp membrane was noticeably improved. Cell viability and adhesion assays revealed that the composite of SF-g-PAA/HAp exhibited acceptable biocompatibility and outstanding adhesion property. The present work provides a novel method for preparation of the fibroin-based biomaterial for bone tissue engineering.
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APPLIED SURFACE SCIENCE
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2017
·
IF 3.387
Ruirui Hao,Guohong Wang,Chuanjia Jiang,Hua Tang,Qingchuan Xu
Semiconductor-based photocatalysis is a promising method for degradation of environmental pollutants, but the activity of most widely used photocatalysts such as titania (TiO 2 ) is still unsatisfactory under visible light. Herein, we synthesized a highly efficient visible-light-responsive heterojunction catalysts based on graphitic carbon nitride (g-C 3 N 4 ) and TiO 2 . The g-C 3 N 4 /TiO 2 heterojunction composites with high specific surface area were prepared via in situ hydrothermal synthesis followed by calcination , using titanium tetrachloride (TiCl 4 ) and melamine as precursors. Interesting, HCl from the hydrolysis of TiCl 4 served as the proton source to acidify the melamine. The g-C 3 N 4 /TiO 2 heterojunction composites exhibited higher photocatalytic performance for decomposition of Rhodamine B (RhB) than pure g-C 3 N 4 or TiO 2 under visible light irradiation. The high activity can be ascribed to the high specific surface area (up to 115.6 m 2 g −1 ) of the g-C 3 N 4 /TiO 2 composites and a synergistic heterojunction structure between TiO 2 and g-C 3 N 4 . Moreover, the photocatalytic performances of the g-C 3 N 4 /TiO 2 composites rely on the content of melamine in the synthesis precursors: with an optimum melamine content (3 g for 0.5 mL of TiCl 4 ), the sample showed the highest photocatalytic performance, which is superior to pure TiO 2 and g-C 3 N 4 by a factor of 18.7 and 3.5, respectively. Active species trapping experiments revealed that superoxide radicals and photogenerated holes played crucial roles in the photocatalytic reactions. The results will provide new ideas for the smart design and development of g-C 3 N 4 -based highly active photocatalysts with ultrahigh specific surface area for environmental and energy applications.
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Journal of Water Process Engineering
·
2024
·
IF 6.3
Yali Song,Ziyan Li,Shuaibin Li,Cairong Yang,Long Huang,Xiaojing Zhang,Qiong Wang,Hongzhong Zhang
Holes and O 2 − have been proved the primary species for sulfamethoxazole (SMX) degradation in g-C 3 N 4 photocatalytic system. On the basis, P doped g-C 3 N 4 (PCN) photocatalyst with midgap states was prepared by the polymerization of urea and etidronic acid, in order to enhance the role of main species in SMX removal via tailoring band gap structure. Compared with g-C 3 N 4 , the energy gap, CB position and VB position of PCN change little, however, PCN shows lower recombination efficiency of photogenerated carriers, strong adsorption and harvest of more visible light due to the midgap states induced by P doping, which is favorable to improve the photocatalytic activity. When the additive amount of etidronic acid is 0.03 g for 10 g urea, the obtained PCN-0.03 exhibits excellent visible-driven photocatalytic ability on SMX degradation. The kinetic constant is 2.1 times than that of pure g-C 3 N 4 . Electron spin resonance tests and trapping experiments imply that OH, holes and O 2 − are produced and enhanced by PCN-0.03 under visible light in comparison with g-C 3 N 4 . Holes and O 2 − take the primary role in SMX photodegradation . The detailed and systematical work may provide an effective strategy for water purification , especially containing SMX.
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ADVANCED FUNCTIONAL MATERIALS
·
2024
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IF 18.5
Mengmeng Chen,Neng Hu,Weijia Wang,Lin Lei,Huiqing Fan,Peter Müller-Buschbaum,Qi Zhong
Inspired from seaweed swayed by waves, the enhanced hydrogen evolution is realized in porous and hybrid g-C 3 N 4 /Pt-PVDF electrospun membranes via piezoelectricity from water flow energy. The membranes are fabricated by dispersing g-C 3 N 4 /Pt into the mixed solution of PVDF and PEO, followed by electrospinning and selective removal of PEO. By changing the PEO amount, the pore size in nanofibers is adjusted. Due to the hydrogen bonding between g-C 3 N 4 /Pt and PVDF, the β phase of PVDF is increased, beneficial for the piezoelectricity performance. When the electrospun membranes are exposed to water flow, an additional potential field is triggered due to the deformation of PVDF. It not only eases the photogeneration of charge carriers from g-C 3 N 4 /Pt but also hinders their recombination. The prolonged lifetime significantly improves the photocatalytic water splitting of g-C 3 N 4 /Pt under visible light. The hydrogen evolution in the electrospun membranes (PVDF to PEO = 4:1) is profoundly improved to 9 278 µmol h −1 g −1 , almost doubled to the pure g-C 3 N 4 /Pt nanosheets (5 220 µmol h −1 g −1 ). Therefore, the seaweed-inspired electrospun membrane is a promising strategy for the efficiently photocatalytic water splitting via g-C 3 N 4 in an aqueous environment, such as a natural sea and lake, by the piezoelectricity gained from the water flow energy.
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Environmental Science-Nano
·
2025
·
IF 5.1
Qi Xu,Xu Du,Qin Zhou,Lian Yang,Kuixiao Li,Wei Wang,Gang Wang,Guisheng Xu,Feng Xiao
Photocatalytic oxidation technology is recognized as an effective method for removing antibiotics from wastewater. In this work, a novel montmorillonite/graphitic carbon nitride (Mt/g-C3N4) composite was synthesized via a facile thermal polycondensation approach. The optimal 1% Mt/g-C3N4 photocatalyst demonstrated an excellent chlortetracycline (CTC) degradation rate of 0.0353 min-1 under visible light, which is about 42% higher than pristine g-C3N4. Electron spin resonance (ESR) and radical quenching experiments revealed that the •O2- plays the most significant role in the photocatalytic oxidation of CTC, while •OH, 1O2 and h+ also contribute, but not in dominant places. This study elucidates the mechanism by which the photocatalytic activity is enhanced. The improvements are ascribed to the extended absorption of visible light, efficient charge separation, and the inhibition of photo-induced electron-hole pair recombination, following the successful intercalation of g-C3N4 nanosheets into montmorillonite layers. Additionally, HPLC-QTOF-MS was employed to identify the transformation products of CTC, and the primary degradation pathways were elucidated. Furthermore, the ecotoxicity of the transformation products was evaluated using ECOSAR software.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2025
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IF 6.3
Fei Gao,Weijun Chen,Ye Li,Long Shao,Li He
Organic-inorganic hybrid perovskite films with excellent surface morphology and large grain sizes are crucial for achieving high-performance perovskite solar cells (PSCs). To reduce production costs and meet the growing demand for lightweight thin solar cells, developing ultra-thin absorber layers is an effective approach. However, defects and trap sites are inevitably formed on the surfaces of solution-processed perovskite films. Herein, we present an innovative method that directly incorporates graphite carbon nitride/carbon quantum dots (g-C 3 N 4 /CQDs) into the perovskite precursor solution, using them as structural template to modulate film growth. The g-C 3 N 4 /CQDs delay crystallization through the formation of a MA·g-C 3 N 4 /CQDs intermediate adduct, which reduces light absorption loss by increasing enlarge grain size and minimizing numerous pinhole defects in thin perovskite films. Additionally, the amide bond formed between g-C 3 N 4 and CQDs can accelerate charge transport, as demonstrated by density functional theory calculation. As a result, the PSCs based on high-quality 360 nm MAPbI 3 :g-C 3 N 4 /CQDs films achieved a champion power conversion efficiency of 20.49 %. The devices retained more than 90 % of their initial efficiency for over 500 h in air without encapsulation. This work not only provides valuable insights into the role of 2D/0D carbon-based heterojunctions in PSCs, but also provides a straightforward technique for crystallization regulation to achieve efficient and stable PSCs.
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DIAMOND AND RELATED MATERIALS
·
2025
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IF 5.1
Gang Huang,Danlin Zeng,Mengcheng Wei,Yang Chen,Aoxue Ma,Sirong Cheng
A novel nonmetal-based nanocomposite, protonated g-C 3 N 4 @CaCO 3 (CN-BPCC), was synthesized using a simple calcination method. The construction of this binary composite photocatalyst enhanced the adsorption and photocatalytic degradation performances of pure g-C 3 N 4 . The adsorption and photocatalytic degradation efficiencies under visible-light irradiation were analyzed using methylene blue (MB) as the target organic pollutant. The binary nanocomposite with 1.5 g of CaCO 3 (BPCC) exhibited high adsorption and photocatalytic performance, achieving approximately 100 % degradation of MB under 90 min of xenon lamp irradiation. Furthermore, the nanocomposite displayed enhanced visible-light utilization efficiency and improved separation efficiency of photogenerated carriers. The adsorption and photocatalytic degradation mechanisms were investigated through radical trapping experiments and electron spin resonance (ESR) spectroscopy. Furthermore, the binary CN-BPCC nanocomposite exhibited exceptional reusability after regeneration, indicating its potential as a promising photocatalyst for the treatment of wastewater containing organic pollutants.
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