ENVIRONMENTAL RESEARCH
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2021
·
IF 6.498
Yongli Liu,Xiaoyan Su,Letian Chen,Huanjia Liu,Chunyuan Zhang,Jiali Liu,Jiayi Hao,Yu Shangguan,Guifen Zhu
p -Aminoazobenzene ( p AAB) is a hazardous azo dye that causes considerable harm to human health and the environment. The development of novel and sensitive sensors for the rapid detection of p AAB is in high demand. In this study, a simple fluorescent sensor for p AAB detection is designed based on carbon dots (CDs) which are prepared using green carbon source of Momordica charantia L. via a facile hydrothermal approach. The fluorescence spectra of CDs exhibit considerable overlap with the absorption band of p AAB, and the fluorescence is specifically suppressed in the presence of p AAB ascribed to the inner filter effect. Good and wide linearity is observed in the p AAB concentration range of 0.01–12.5 μg mL −1 with a lower detection limit of 3.9 ng mL −1 . The established method achieves good results with a rapid analysis of p AAB in different practical water and soil samples. The as-constructed fluorescent sensor provides a simple, rapid, economical and eco-friendly platform and possesses prospective applications for the effective, selective and sensitive detection of p AAB in the environmental field.
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2019
·
IF 3.131
Feng Su,Peng Yun,Chenglong Li,Rongye Li,Laishun Xi,Yuandou Wang,Yangsheng Chen,Suming Li
A series of poly(2-ethyl-2-oxazoline)-poly(L-lactide) (PEOz-PLA) diblock copolymers were synthesized by ring-opening polymerization of l -lactide using a monohydroxy terminated PEOz-OH macro-initiator in the presence of stannous octoate as catalyst. The resulting diblock copolymers were characterized by 1 H nuclear magnetic resonance and gel permeation chromatography . Self-assembled micelles were prepared using the co-solvent evaporation method in water and in phosphate buffered saline (PBS) at pH 7.4, 6.5 and 5.0. The resulting micelles exhibit different morphologies, such as spherical micelles and worm-like micelles depending on the hydrophilic/hydrophobic balance. Spherical micelles were exclusively observed for PEOz-PLA copolymers with short PLA blocks, whereas co-existence of worm-like and spherical micelles was observed for copolymers with long PLA blocks. The micelle size increases with decreasing pH due to the electrostatic repulsion between PEOz chains resulting from ionization of the tertiary amide groups along PEOz chains. A hydrophobic anti-tumor drug, paclitaxel , was entrapped in PEOz-PLA micelles. High loading efficiency up to 86.7% was obtained for copolymers with long PLA blocks. Drug release was performed in PBS at different pH values. During the 30-day release period, faster release was obtained for copolymers with shorter PLA blocks than for copolymers with longer PLA blocks, at acidic pH than at pH 7.4. It is thus concluded that pH-responsive PEOz-PLA copolymer micellss could be promising as carrier of anti-tumor drugs.
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高影响因子
BIOSENSORS & BIOELECTRONICS
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2013
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IF 5.437
Xiaoyu Mu,Li Qi,Ping Dong,Juan Qiao,Jian Hou,Zongxiu Nie,Huimin Ma
Gold nanoclusters (Au NCs) possess outstanding physical and chemical attributes that make them excellent scaffolds for the construction of novel chemical and biological sensors. In this study, a simple one-pot synthesis method, employing l -proline as the stabilizer, was presented for preparation of fluorescent Au NCs. This strategy allowed the generation of water-soluble Au NCs within a short time of 15 min. The as-prepared Au NCs exhibited a bluish fluorescence emission at 440 nm and a quantum yield of 2.94%. Based on the aggregation-induced fluorescence quenching mechanism, the Au NCs provided favorable biocompatibility, high sensitivity and good selectivity for the measurement of ferric ion (Fe 3+ ). Furthermore, serum samples were analyzed for the serum iron contents by using this proposed biocompatible fluorescent sensor, indicating the potential value of this Au NCs-based fluorescent sensor for application in biological and clinical analysis.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2012
·
IF 3.898
Liqiang Luo,Fang Li,Limei Zhu,Yaping Ding,Dongmei Deng
A novel poly( l -proline)-ordered mesoporous carbon film with controllable thickness on the glassy carbon electrode surface was fabricated by one-step electrochemical technique and further used for the construction of electrochemical sensing platform of natural estrogens. Scanning electron microscopy, Fourier transform infrared spectroscopy and electrochemical impedance spectroscopy were employed to characterize the surface morphology , structure and interface property of the polymer. The voltammetric behaviors of estrogens involving estradiol, estrone and estriol were investigated on the modified electrode , and the proposed electrode exhibited strong electrocatalytic activity toward the oxidation of three estrogens especially for estradiol, making it a promising electrochemical sensing platform for sensitive detection of estrogens. Under the optimum conditions, the linear range of estradiol obtained by square-wave voltammetric determination was 1.0 × 10 −8 –2.0 × 10 −6 mol/L with a low detection limit of 5.0 × 10 −9 mol/L. The developed modified electrode was also applied to the determination of estradiol in female blood serums with satisfactory results.
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DOI
高影响因子
Carbohydrate Polymer Technologies and Applications
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2021
·
IF 0
Yiru Wang,Wenrong Qin,Qi Liang,Feng Zhou,Chengyun Yan,Yueyi Deng
The purpose of this study was to enhance quercetin (Que) dissolution by preparing its ternary amorphous solid dispersions. The ternary amorphous solid dispersions were prepared by solvent evaporation method using hydroxypropylmethylcellulose acetate succinate (HPMCAS) and L-lysine (lys) as the carriers. The effect of the combination of polymer and amino acid on nucleation inhibition was evaluated by the nucleation induction time. Supersaturation tests were used to investigate the ability of maintaining Que supersaturation of the combination of polymer and lys. The dissolution results showed that the dissolution effect of ternary solid dispersions was better than binary solid dispersions. Stronger crystallization inhibition didn't lead to better dissolution effect and the dissolution of the prepared amorphous solid dispersions of Que was carrier-controlled. Lys modified the dissolution microenvironment of the solid dispersions, which made drug dissolution in the optimal pH environment, and effectively improved drug dissolution. Polymer/lys can be one of the most potential carriers for preparing amorphous solid dispersions of Que.
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FOOD RESEARCH INTERNATIONAL
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2024
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IF 7
Xueying Wang,Ziqi Sun,Xiaoli Wang,Minjie Li,Boru Zhou,Xiaoshu Zhang
Solanum nigrum L. (SN) berry is an edible berry containing abundant polyphenols and bioactive compounds, which possess antioxidant and antiinflammatory properties. However, the effects of SN on alcohol-induced biochemical changes in the enterohepatic axis remain unclear. In the current study, a chronic ethanol-fed mice ALD model was used to test the protective mechanisms of SN berries. Microbiota composition was determined via 16S rRNA sequencing, we found that SN berries extract (SNE) improved intestinal imbalance by reducing the Firmicutes to Bacteroides ratio, restoring the abundance of Akkermansia microbiota, and reducing the abundance of Allobaculum and Shigella . SNE restored the intestinal short-chain fatty acids content. In addition, liver transcriptome data analysis revealed that SNE primarily affected the genes involved in lipid metabolism and inflammatory responses. Furthermore, SNE ameliorated hepatic steatosis in alcohol-fed mice by activating AMP-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC), peroxisome proliferator-activated receptor α (PPAR-α). SNE reduced the expression of toll-like receptor 4 (TLR4), myeloid differentiation factor-88 (MyD88) nuclear factor kappa-B (NF-κB), which can indicate that SNE mainly adjusted LPS/TLR4/MyD88/NF-κB pathway to reduce liver inflammation. SNE enhanced hepatic antioxidant capacity by regulating NRF2-related protein expression. SNE alleviates alcoholic liver injury by regulating of gut microbiota, lipid metabolism, inflammation, and oxidative stress. This study may provide a reference for the development and utilization of SN resources.
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高影响因子
Small
·
2024
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IF 13
Lihua Chen,Hui Ming,Bowen Li,Chen Yang,Shanshan Liu,Yajie Gao,Tingting Zhang,Canhua Huang,Tingyuan Lang,Zhuo Yang
Cancer metastasis poses significant challenges in current clinical therapy. Osthole (OST) has demonstrated efficacy in treating cervical cancer and inhibiting metastasis. Despite these positive results, its limited solubility, poor oral absorption, low bioavailability, and photosensitivity hinder its clinical application. To address this limitation, a glutathione (GSH)-responded nano-herb delivery system (HA/MOS@OST&L-Arg nanoparticles, HMOA NPs) is devised for the targeted delivery of OST with cascade-activatable nitric oxide (NO) release. The HMOA NPs system is engineered utilizing enhanced permeability and retention (EPR) effects and active targeting mediated by hyaluronic acid (HA) binding to glycoprotein CD44. The cargoes, including OST and L-Arginine (L-Arg), are released rapidly due to the degradation of GSH-responsive mesoporous organic silica (MOS). Then abundant reactive oxygen species (ROS) are produced from OST in the presence of high concentrations of NAD(P)H quinone oxidoreductase 1 (NQO1), resulting in the generation of NO and subsequently highly toxic peroxynitrite (ONOO − ) by catalyzing guanidine groups of L-Arg. These ROS, NO, and ONOO − molecules have a direct impact on mitochondrial function by reducing mitochondrial membrane potential and inhibiting adenosine triphosphate (ATP) production, thereby promoting increased apoptosis and inhibiting metastasis. Overall, the results indicated that HMOA NPs has great potential as a promising alternative for the clinical treatment of cervical cancer.
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DOI
PubMed
高影响因子
JOURNAL OF BIOTECHNOLOGY
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2015
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IF 2.871
Xia Yi,Peng Zhang,Jiaoe Sun,Yi Tu,Qiuqiang Gao,Jian Zhang,Jie Bao
Pediococcus acidilactici TY112 producing l -lactic acid and P. acidilactici ZP26 producing d -lactic acid, were engineered from the wild-type P. acidilactici DQ2 by ldhD or ldh gene disruption , and the robustness of the wild-type strain to the inhibitors derived from lignocellulose pretreatment was maintained well. In simultaneous saccharification and fermentation (SSF), 77.66 g L −1 of l -lactic acid and 76.76 g L −1 of d -lactic acid were obtained at 25% (w/w) solids content of dry dilute acid pretreated and biodetoxified corn stover feedstock. l - and d -Lactic acid yield and productivity were highly dependent on the inhibitor removal extent due to the significant down-regulation on the expressions of ldh and ldhD encoding lactate dehydrogenase by inhibitor, especially syringaldehyde and vanillin at the low concentrations. This study provided a prototype of industrial process for high titer l - and d -lactic acid production from lignocellulose feedstock.
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高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2020
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IF 5.774
Lingfeng Wu,Lei Li,Shengjie Chen,Lu Wang,Xue Lin
In this study, an ultrasonic-assisted extraction (UAE) procedure with selected deep eutectic solvents (DES) as solvent was first designed to simultaneously optimize the total phenolic/flavonoid content (TPC/TFC) and antioxidant activities of Moringa oleifera L. leaves (MOLs) by using response surface methodology (RSM). The key factors for RSM were selected based on the design of the experimental results along with a three-factors-five-level, central composite design (CCD), including 20 experimental runs. The analysis of variance (ANOVA) results revealed that the water content in DES had a significant influence on all responses, while the ultrasonic time and the ratio of liquid to solid had no statistically significant effects on the total phenolic content. The optimal conditions of the combination of TPC/TFC and antioxidant activities were obtained as follows: 37% water content in DES, 144 W ultrasonic power, and 40 °C ultrasonic temperature. The measured parameters corresponded with the predicted results. Moreover, a comparative study confirmed that the optimized DES-based UAE yielded further higher TPC, TFC, and antioxidant activities than other extraction methods. The results of HPLC analysis in optimized conditions verified that the MOLs extracts with DES-based UAE included 14 phenolic compounds with high concentrations of vicenin-2 (17.6 mg/g) and orientin (23.6 mg/g). The present study supplied a green and high-efficient method for extracting high levels of anti-oxidative phenolic compounds from MOLs.
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高影响因子
African Journal of Pharmacy and Pharmacology
·
2017
·Vol. 12
·期 47
·582-590
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IF 0
Raphael N. Alolga,Enos Mais,Vitus Onoja
The quality of botanicals has been reported to be influenced by their places of cultivation. Although, ginger remains a common health remedy both in Ghana and China, no comparative study exists to the best of the authors’ knowledge, on the anti-inflammatory properties of ginger from these two countries. The aim of this study was to comparatively assess the anti-inflammatory effect of the methanolic extracts of fresh ginger samples from Ghana and China and identify biomarker(s) that is/are diagnostic of acute inflammation via untargeted metabolomics. A phytochemical assessment of the ginger extracts was initially performed with high performance liquid chromatography- quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS). Low (200 mg/kg) and maximum (400 mg/kg) doses the ginger extracts from each country were assessed using Sprague-Dawley (SD) rats against groups for positive (indomethacin, 10 mg/kg) and negative controls (no drug). Blood samples were taken from the retro orbital vein at four time points: 1, 4, 8 and 24 h after induction of inflammation. The levels of tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2) were determined in the plasma samples. The metabolomics study was also performed with HPLC-QTOF/MS and the data analyzed with MetaboAnalyst 3.0 and SIMCA 14.1. Phytochemical evaluation revealed marked differences in terms of the compounds deemed to have anti-inflammatory activities in the ginger samples from the two countries. Ginger extract from Ghana showed superior anti-inflammatory effects over that from China. In the metabolomics study, L-valine was identified as the diagnostic biomarker of acute inflammation from 8 differential metabolites identified.
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Polymers
·
2022
·Vol. 14
·期 12
·2409
·
IF 4.967
Ngoc-Thang Nguyen,Thi-Lan-Huong Vo
The current work presented a green synthetic route for the fabrication of silver nanoparticles obtained from aqueous solutions of silver nitrate usingCordyline fruticosaL. leaf extract (Col) as a reducing and capping agent for the first time. The bio-synthesized silver nanoparticles (AgCol) were investigated using UV–visible spectroscopy (UV–vis), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and thermal gravimetric analysis (TGA). The obtained data demonstrated that AgCol in spherical shape with an average size of 28.5 nm were highly crystalline and well capped by phytocompounds from the Col extract. Moreover, the bio-synthesized AgCol also exhibited the effective antibacterial activities against six pathogenic bacteria, includingEscherichia coli(E. coli),Pseudomonas aeruginosa(P. aeruginosa),Salmonella enterica(S. enterica),Staphylococcus aureus(S. aureus),Bacillus cereus(B. cereus)and Enterococcus faecalis(E. faecalis). The AgCol were applied as an antibacterial finishing agent for viscose fabric using a pad-dry curing technique. The AgCol-treated viscose fabrics exhibited a good synergistic antimicrobial activity againstE. coliandS. aureusbacteria. Furthermore, the silk fibroin regenerated fromBombyx moricocoon waste was utilized as an ecofriendly binder for the immobilization of AgCol on the viscose fabric. Thus, the antimicrobial efficacy of the AgCol and fibroin modified viscose fabric still reached 99.99% against the tested bacteria, even after 30 washing cycles. The colorimetric property, morphology, elemental composition, and distribution of AgCol on the treated fabrics were investigated using several analysis tools, including colorimetry, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), atomic absorption spectroscopy (AAS), Kjeldahl, and FTIR. Because of the excellent antimicrobial efficiency and laundering durability, as well as the green synthesis method, the AgCol and fibroin modified viscose fabric could be utilized as an antibacterial material in sportswear and medical textile applications.
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高影响因子
Biomedical Physics & Engineering Express
·
2022
·Vol. 8
·期 3
·035002
·
IF 0
Siqi Li,Xiaoling Yu,Yuan Li,Tian Zhang
For nerve tissue engineering (NTE), scaffolds with the ability to release drugs under control and support the rapid proliferation of cells are very important for the repair of nerve defects. This study aimed to fabricate a conductive drug-loaded fiber mat by electrospinning and assess its potential as a scaffold for Schwann cells proliferation. The conductive polypyrrole (PPy) was coated on an electrospun poly (D, L-lactide) (PLA) fibrous mat, which was simultaneously embedded with protein-loaded chitosan nanoparticles and ibuprofen as a model small molecule drug. The fibrous mat shows suitable conductivity, mechanical properties, and hydrophilicity for NTE. For drug release and degradation studies, the fibrous mat can achieve sustained release of bovine serum albumin (BSA) and ibuprofen, and the PPy coating can increase the surface wettability and conductivity while slowing down the degradation of the fibrous mat. The application of electrical stimulation (ES) to the fibrous mat can accelerate the release of ibuprofen, but there was no significant effect on the release rate of the protein. The fibrous mat showed no cytotoxicity in vitro, and Schwann cells (SCs) can adhere, grow, and proliferate well on mats. At the 120th hour of culture in vitro, the relative growth rate of SCs on the conductive drug-loaded fibrous mat reached 198.22 ± 2.34%, which was an increase of 37.93% compared to the SCs on the drug-loaded fibrous mat with ES. The density and elongation of SCs on the conductive drug-loaded fibrous mat were greater than those on the PLA fibrous mat, indicating that the conductive polypyrrole-coated electrospun chitosan nanoparticles/PLA fibrous mat has good potential for application in nerve regeneration.
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