BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Hao-Cheng Hu,Shao-Hua Wu,Lei-Xin Jin,Jian-Jun Sun
In this work, a novel “turn-on” mode Au nanocubes (AuNCs) enhanced surface-enhanced Raman scattering (SERS) biosensing platform coupled with heated Au electrode (HAuE) and strand displacement amplification (SDA) strategy was proposed for highly sensitive detection of DNA adenine methylation (Dam) Methyltransferase (MTase) activity. The Dam MTase and Dpn I enzyme activities were significantly increased by elevating the HAuE surface temperature, resulting in the rapid production of template DNA for later SDA. During the SDA process, the released single-stranded DNA (ssDNA) could be amplified exponentially, and its concentration was positively related to the Dam MTase activity. The plasmonic AuNCs in SERS tags could provide significant SERS enhancement due to their “lightning rod” effect resulting from the sharp feature of the edges and corners of AuNCs. Because of these factors, the proposed biosensors exhibited high sensitivity in detecting the Dam MTase activity. The limit of detection was estimated to be 8.65 × 10 −5 U mL −1 , which was lower than that in most of the sensors for detection of Dam MTase activity in the literature. This SERS biosensor could also be used to screen inhibitors of Dam MTase and had the potential for detecting Dam MTase activity in real biological samples.
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Food Analytical Methods
·
2026
·Vol. 19
·期 12
·388
·
IF 3
Deng Guican,Bao Yonghua,Hong Yanping,Hong Jiaxin,Duan Luying,Du Huaying,Tan Zhimei,Wang Chunrong
As illegal synthetic colorants in food, Sudan dyes possess carcinogenic potential and can severely threaten human health following long-term intake. Rapid and sensitive detection of Sudan dyes in complex food matrices (e.g., chili powder and tomato sauce) remains a critical challenge for food safety supervision. Herein, a facile and sensitive detection strategy targeting Sudan I, II, III, and IV was established by combining magnetic substrates with surface-enhanced Raman spectroscopy (SERS). The influence of Au NPs loading on SERS analytical performance was systematically investigated, and FSA-3 was identified as the optimal substrate. Under optimized conditions, the limits of detection (LODs) for Sudan I–IV in chili powder and tomato sauce were determined to be 0.75–11.5 μg/kg, with satisfactory recoveries ranging from 84.5% to 110%. Compared with previously reported SERS methods and conventional analytical techniques, the proposed method exhibits excellent resistance to matrix interference capability and enables sensitive and efficient detection. This work provides a reliable and efficient alternative for the rapid screening of Sudan dye residues in complex food matrices, holding great promise for practical food safety applications.
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TALANTA
·
2018
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IF 4.244
Linlin Xu,Hua Zhang,Yue Tian,Anxin Jiao,Feng Chen,Ming Chen
The prospect of wielding surface-enhanced Raman spectroscopy (SERS) as a powerful technique for ultrasensitive detection of organic molecules in wastewater has received extensive attention in environmental surveillance. Based on ultraviolet (UV, 405 nm) laser irradiation of ZnO nanorods in HAuCl 4 solution, ZnO@Au nanorods with controllable Au nanoparticles were successfully fabricated and established as an advanced SERS-based substrate. The reduction of Au ions was driven by the generation of electron-hole pairs via UV laser excitation of semiconductor-based nanomaterials , resulting in the moderate overgrowth of Au nanoparticles on the ZnO nanorods. The Au composition-dependent SERS analysis of crystal violet (CV) molecules revealed that the ZnO@Au nanorods with 16.21% Au contents exhibited optimized SERS activity in comparison with other nano-substrates in this paper. Furthermore, the detection limit of light-resistant methyl blue (MB) dye molecules was achieved at nanomole (nM) level of 10 −9 M (0.8 μg/L), providing ultrasensitive detection of organic pollution in wastewater. Even after twenty recycles, the excellent reusability of this novel substrate with 65% original SERS intensity was achieved by subsequently eliminating the residual MB molecules via photocatalytic degradation. Therefore, the as-prepared ZnO@Au nanorods can serve as a cost-effective, clean, reusable and active SERS substrate for ultrasensitive monitoring of light-resistant organic pollutant in natural ecosystems.
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APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Lin Zhu,Xiaoyue Su,Hao Ma,Lianze Shan,Bing Zhao
Spectra in surface-enhanced Raman scattering (SERS) are always accompanied by a continuous emission known as “background”, which complicates the analysis with a poor signal-to-noise ratio (SNR), especially for qualitative and quantitative detection. Here, the SERS profile of the CH 3 NH 3 PbBr 3 substrate displays high similarity with the bulk Raman spectra, and its FWHM is exceeding narrow. The significantly increased SNR of the probe molecules indicates that the substrate helps to improve the quantitative analysis capability of the SERS technology and alleviate the background-related problems. For the CH 3 NH 3 PbBr 3 substrate, good linearity (R 2 = 0.9591) in the range of 1.0 × 10 -7 − 1.0 × 10 -3 mol/L was obtained and the limit of detection (LOD) is 7.88 × 10 -6 mol/L. The reason for this excellent performance is that the introduction of organic molecules splits the 3D CH 3 NH 3 PbBr 3 substrate to form a 2D perovskite that fixes the molecular orientation through chemical interactions. The CH 3 NH 3 PbBr 3 has the advantage of maintaining the conformation and orientation consistency of adsorbed molecules. Meanwhile, compared with the commonly used noble metal (Au, Ag) and semiconductor (ZnO, TiO 2 ) SERS substrates, the CH 3 NH 3 PbBr 3 substrate has obvious superiorities in both spectral peak intensity and peak shape. Furthermore, the CH 3 NH 3 PbBr 3 substrate possesses specific recognition ability that has the same enhancement effect for a series of pyridine-containing probe molecules. Finally, the enhancement mechanism is discussed according to the CT contribution. As a promising substrate for SERS technology, CH 3 NH 3 PbBr 3 will provide more possibilities for quantitative and multiplex detection in the future.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 9
·1-10
·
IF 5.705
Sun Ying,Wang Ruixue,Liu Xuan,Shan Guiye,Chen Yanwei,Tong Ti,Liu Yichun
Platinum nanoparticles (PtNPs) were uniformly grown on the surface of gold nanorods (AuNRs) by a laser irradiation procedure. Transmission electron microscopy confirmed that the PtNPs are uniformly grown on the surface of the AuNRs. The formation of PtNPs on the AuNRs leads to a red-shift of the absorption maximum from 734 nm to 766 nm. In addition, the efficiency of surface enhanced Raman scattering (SERS) is increased, but the photothermal conversion efficiency is decreased compared to pure AuNRs. The result indicates that electron transfer occurs between gold and platinum. The peroxidase mimicking effect of PtNPs, AuNRs and Au/Pt NRs by catalyzing the oxidation of colorless 3,3’,5,5’-tetramethylbenzidine (TMB) to blue oxidized 3,3’,5,5’-tetramethylbenzidine (oxTMB; a quinone) in the presence of H 2 O 2 . The catalytic activity of Au/Pt NRs is higher than that of sole AuNRs or PtNPs by factors of 4.2 and 2.1, respectively. Thus, Au/Pt NRs have been used for the detection of peroxide and the limit of detection is 0.04 μM. This work provides an approach to integrate the peroxidase mimicking effect with SERS enhancement for potential application in detection.
A schematic diagram for the laser-induced growth of Au/Pt NRs and the colorimetric determination of hydrogen peroxide concentration with their peroxidase mimicking properties. The limit of detection is 0.04 μM based on the use of Au/Pt NRs as a catalyst.
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Journal of Materials Chemistry C
·
2021
·Vol. 9
·期 28
·9011-9020
·
IF 7.393
Jiangcai Wang,Cuicui Qiu,Hua Pang,Junyu Wu,Mengtao Sun,Dameng Liu
The ultrasensitive surface-enhanced Raman spectroscopy (SERS) detection of trace organic pollutants is an important issue in environmental protection. However, the SERS detection performance easily suffers from poor sensitivity and reproducibility due to the limitations of materials. A high-performance substrate is necessary to control the plasmonic hotspots in a wide spectral range. In this work, a perovskite quantum dot–graphene/nano-Au (PQD–G/Au) composite was designed using the combination of ligand-assisted reprecipitation and spinning coating techniques. The SERS performance of PQD–G/Au composites showed a strong dependence on their synthesis parameters, which was proved by the finite-difference time-domain program simulation. An excellent SERS detection behavior was obtained on the PQD–G/Au composite for rhodamine 6G (R6G) and p-nitrophenol (PNP) with a large linear detection range (10−12 to 10−6 M for R6G and 10−8 to 10−4 M for PNP) and a low detection limit (6.02 × 10−13 M for R6G and 2.95 × 10−9 M for PNP). The superior SERS performance was attributed to the synergistic effect of the electromagnetic enhancement of Au nanostructures and chemical enhancement of PQD–G heterostructures. The proposed method paves a new practical application of other 2D materials and heterostructures for designing a novel PQD-based SERS substrate.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Jiana Lin,Ying Chen,Gongke Li,Yuling Hu
Monitoring seafood freshness is critical for ensuring nutritional value and food quality, but targeted analysis in complex matrices remains challenging. Herein, a thiol-functionalized calix[6]arene covalent organic framework grafted with AuNPs, denoted as CX6-SCOF-AuNPs, was strategically designed for rapid, sensitive and selective detection of tryptamine in seafood by surface-enhanced Raman spectroscopy (SERS). CX6-SCOF-AuNPs featured extensive pocket-like hydrophobic cavities integrated within an ordered porous structure, enabling specific capture of tryptamine with exceptional enrichment capacity (203.4 mg/g) and rapid adsorption kinetics (within 2 min). The selectivity mechanism stemmed from the formation of a ground-state charge-transfer complex driven by specific “pocket effect” between CX6-SCOF and size-matched tryptamine. CX6-SCOF-AuNPs was successfully applied to enrichment and detection of tryptamine in seafood, achieving high sensitivity (LOD of 3.4 μg/L) and satisfactory recoveries (90.6 % – 107.4 %). This work provides a promising strategy for supramolecular recognition and targeted SERS analysis of trace hazards in complex food matrices.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Xingliang Cheng,Ying Chen,Gongke Li,Yuling Hu
Nanozymes, as catalytic nanomaterials mimicking natural enzymes, have emerged as powerful tools for analytical sensing. However, conventional methods for nanozyme synthesis and immobilization often suffer from time-consuming processes and inefficient substrate integration. Herein, we develop an innovative electrochemical strategy that achieves simultaneous synthesis and immobilization of sponge-like two-dimensional tetrakis(4-carboxyphenyl)porphyrin-Fe (Cu-TCPP(Fe)) nanozymes (termed as STCTFzymes) on Cu foils within only 6 min. Kinetic analysis confirms that the electrochemical growth follows a diffusion-controlled instantaneous nucleation mechanism. The resulting STCTFzymes exhibit enhanced operational stability enabled by firm substrate anchoring and facile recyclability through physical retrieval of the copper support. Leaching tests and post-recycling structural analysis further validate the excellent structural integrity and anti-leaching capability of STCTFzymes. Given the excellent peroxidase-like (POD) activity of STCTFzymes, a surface-enhanced Raman scattering (SERS) method based on aptamer-specific recognition was developed using ochratoxin A (OTA) as a model analyte. The OTA aptamer was adsorbed on the surface of STCTFzymes to block the catalytic site and turn off the POD-like activity. Upon specific binding of OTA to the aptamer, the enzyme activity of STCTFzymes was reactivated, resulting in a favorable change in SERS signal. The established method showed a wide linear range (0.3–500 ng/mL) and low detection limit (0.091 ng/mL) for OTA, with superior selectivity, high sensitivity and satisfactory stability. This electrochemically-assisted self-assembly provides a new approach for developing robust nanozymes architectures on heterogeneous substrates. When synergistically integrated with aptamer-based recognition, the STCTFzymes presents a universal strategy for sensitive detection of biotoxins exhibiting inherently weak Raman response.
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Analytical Methods
·
2025
·
IF 2.6
Zixun Wang,Dong Zhang,Yuxuan Wu,Xu Ye,Yayun Qian,Fengsong Chen
The low early diagnosis rate of hepatocellular carcinoma (HCC) severely impacts patient prognosis, making the development of highly sensitive and specific early diagnostic technologies crucial. MicroRNA-221 (miR-221), an aberrantly overexpressed biomarker in HCC, holds significant diagnostic potential. This paper constructed a capillary surface-enhanced Raman scattering (SERS) sensing platform utilizing CRISPR/Cas13a trans-cleavage and double-shell gold-silver nanorods (DS Au-AgNRs) to detect serum miR-221 in HCC patients. DS Au-AgNRs were synthesized and assembled onto aminated capillaries, followed by conjugation of Cy5-labeled single-stranded DNA (ssDNA) to the DS Au-AgNR surface via Au–S bonds. In the presence of miR-221, activated CRISPR/Cas13a trans-cleavage cleaves the ssDNA, releasing Cy5 from the sensor surface and diminishing the SERS signal, enabling miR-221 quantification. The synthesized DS Au-AgNRs exhibit uniform morphology and size, are uniformly distributed on the capillary, and form numerous “hotspots”, thereby significantly enhancing the SERS signal. According to the characteristic peak of Cy5 at 1074 cm−1, a linear relationship is established between the log concentration of miR-221 and the measured SERS intensity (y = −3527.97 × −35369.60, R2 = 0.97767), with a LOD as low as 4.17 × 10−17 M. The sensor demonstrated high specificity and high sensitivity, and its capacity to detect miR-221 expression aligned with qRT-PCR results when analyzing serum samples, confirming that hepatocellular carcinoma patients exhibited significantly higher miR-221 levels compared to healthy individuals. The capillary SERS sensor thus provides an accurate and convenient approach for early HCC diagnosis.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Xin Zhao,Shibin Han,Cheng Chen,Xiaoyi Lv,Jiahe Li,Chen Chen
Celecoxib (CXB) is a non-steroidal anti-inflammatory drug used to prevent and treat arthritis. However, overdosing or improper use can cause adverse reactions. SERS is an important tool for therapeutic drug monitoring (TDM).The present study proposes an innovative detection scheme based on SERS deep learning, aiming to achieve qualitative identification and quantitative detection of CXB at the Raman spectrum level. In this paper, a composite SERS substrate consisting of silver (Ag)@ platinum (Pt)@ porous silicon Bragg mirror (PSB) was synthesized. Crystal violet (CV) was used as probe to characterize the substrate properties, and the substrate showed excellent SERS properties and long-term stability. The substrate significantly enhanced the SERS response capacity to CXB, showing excellent reproducibility and high sensitivity. The LOD and the recovery rate were both within the reasonable range when the substrate was used to detect CXB concentration in serum. Three deep learning models, GoogleNet, ResNet and VGG, were used to classify five kinds of blood drug concentrations. Among them, the GoogleNet model based on spectral dataset achieved the highest classification accuracy of 84.17 %. The deep learning-SERS integrated system supports blood drug concentration discrimination, helping doctors personalize treatment strategies for arthritis patients and improving clinical efficiency.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Luyun Xu,Yongshi Shen,Zhizhong Lin,Jianqing Ye,Lingna Wang,Xueliang Lin,Shuhang Liu,Duo Lin,Yuanji Xu,Zongyang Yu,Rongcai Zhang,Shangyuan Feng
Lung cancer remains the leading cause of cancer-related deaths worldwide, mainly due to challenges in early detection that often result in poor patient outcomes. In this study, we developed a magnetic biosensing platform based on the coupling of CHA and SERS technologies for one-step detection of lung cancer-related circRNAs. Our approach capitalizes on forming “hot spots” between core–shell SERS nanoprobes and magnetic nanoparticles with precisely defined functionalities, generating a robust Raman signal. This signal is further amplified through magnetic enrichment and calibrated with an internal standard molecule, achieving a remarkably sensitive detection limit of 5.5 aM. Notably, our circRNA detection method significantly surpasses conventional tumor markers’ sensitivity. Validation using human serum samples demonstrated considerably higher circRNA levels in lung cancer patients than healthy controls. Moreover, the core–satellite structured magnetic SERS platform successfully distinguished unique circRNA concentration profiles across early-stage (IA and IB) lung cancers and their subtypes (IA1, IA2, IA3). Collectively, these results underscore the potential of our magnetic SERS platform as a liquid biopsy-based prognostic tool, enhancing early screening sensitivity and thereby facilitating more accurate diagnosis and improved patient stratification.
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Analytical Methods
·
2025
·
IF 2.6
Xiulei Xiong,Haihua Shen,Songnan Liu,Fei Rong,Jian Dong
To detect H2O2 and redox potential of an acute wound simultaneously, a SERS-active microneedle was fabricated by integrating H2O2 and redox potential SERS probes in two grooves of an acupuncture needle, respectively. When the SERS-active microneedle was inserted into tissues, an acute wound formed and the two SERS probes were brought into the wound to sense H2O2 and redox potential of the wound. The feasibility of the SERS-active microneedle was evaluated ex vivo and in vivo. Both sugar deficiency and sugar excess induce the increases of H2O2 and redox potential in most tissues. Short-term physiological disturbances eventually returned to normal levels in healthy organisms, and abnormal sugar intakes disrupt the normal physiological environment and induce stress response in all tissues. The SERS-active microneedle for detection of H2O2 and redox potential would become a powerful tool, used for collecting physiological signals in different tissues to elucidate the mechanisms of wound healing and redox-related diseases.
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