Nature Biomedical Engineering
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2025
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IF 26.6
Xu Saijuan,Liang Dan,Wang Qiudao,Cheng Yan,Xie Da,Gui Yang,Zhang Haokun,Feng Changrui,Zhao Feiyan,Ren Wendan,Sun Gongrui,Yang Yang,Li Lin,Lai Yongrong,Fu Bin,Lu Yuming,Wang Zi Jun,Wu Yuxuan
Ex vivo autologous haematopoietic stem cell (HSC) gene therapy provides a promising treatment option for haematological disorders. However, current methods involve complex processes and chemotherapeutic conditioning, leading to limited accessibility for treatment and major side effects. Here we develop antibody-free targeted lipid nanoparticles (LNPs) for mRNA delivery to HSCs in vivo, enabling efficient base editing of the γ-globin gene ( HBG1/2 ) promoter target in human HSCs to reactivate fetal haemoglobin in derived erythroid cells. Delivery of ABE8e/sgRNA mRNA with optimized LNPs achieves efficient in vivo base editing of HBG1/2 in transfusion-dependent β-thalassaemia (TDT) patient-derived HSCs engrafted in immunodeficient NCG-X mice, showing restored globin chain balance in erythroid cells. Our research indicates that using LNPs for genome editor delivery achieves efficient editing of endogenous genes of human HSCs. This non-viral delivery system eliminates the need for collecting or mobilizing HSCs, providing a potent and one-time treatment potential for blood disorders such as sickle cell disease and TDT.
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Communications Biology
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2025
·Vol. 8
·期 1
·1-17
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IF 5.1
Wang He,Kang Tianyang,Li Weiwei
Gestational diabetes during pregnancy is associated with an increased risk of developmental abnormalities in offspring, but the underlying mechanisms remain unclear. It is not known how maternal metabolism and gut microbes influence kidney development in the fetus. Here we show that gestational diabetes alters maternal gut microbiota and reduces the production of key fatty acids that normally support kidney development in offspring. We find that these changes impair a molecular pathway involving the receptor GPR43 , which promotes the growth and migration of kidney cells. In a mouse model, restoring short-chain fatty acids or transferring gut bacteria from healthy donors improves kidney development in offspring, while blocking GPR43 reverses this effect. This study reveals a previously unknown link between maternal gut metabolism and fetal kidney formation and may guide future strategies to prevent congenital kidney disorders in children born to mothers with gestational diabetes.
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Scientific Reports
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2025
·Vol. 15
·期 1
·1-19
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IF 3.9
Zhu Wenxi,Wang Bingjie,Cui Shengkai,Wang Jing,Tan Huijing
Effective heat transfer between the working fluid and subterranean rocks is essential for producing green and low-carbon geothermal energy. As the primary thermal conductive medium, cement has low thermal conductivity, leading to high thermal resistance and significantly reducing geothermal wells’ efficiency. Therefore, high thermal conductivity cement has emerged as a widely anticipated new research area. The purpose of this research is to address the substantial harm of traditional carbon-based thermal conductive fillers to cement. A novel expanded graphite (EG)/epoxy resin (EP) composite additive (MEG) was designed to increase cement’s thermal conductivity while preserving its mechanical strength and pumpability. Firstly, the physicochemical properties of MEG were revealed by FT-IR, UV-Vis, SEM, and TGA. Then, the applicability of MEG cement in adverse geological environments (high-temperature 60–100 ℃, high-mineralization 5–36% NaCl) was evaluated through simulated maintenance experiments. Finally, the hydration products and pore structure of MEG-cement were analyzed by XRD/FT-IR and SEM/MIP, revealing the thermal conductivity enhancing mechanism. The results showed that: 1) MEG uses ZDMA as a bridge to promote the ring opening and curing of EP, and is formed by strong cation -π interaction with EG. 2)After curing at 60–100 ℃, MEG-cement exhibits a significant increase (46.6-182.1%) in thermal conductivity within the optimal dosage range of 5–10%, fully meeting the requirements for compressive strength (10.4–21.7 MPa) and fluidity (19.3–21.2 cm) of cementing. In addition, MEG-cement maintained stable density and significant high thermal conductivity advantage in high-mineralization environments (5–36% NaCl), with an increase in thermal conductivity of 23.8- 54.1%. 3) The mechanism of MEG promoting heat transfer in cement is summarized as the enhancement of the hydration process and the production of C-S-H gels. C-S-H gels filled the gel pores and transition pores in the cement skeleton and formed a dense, high thermal conductivity network, which shortens the heat transfer path and thus greatly improves the thermal conductivity of cement. In summary, this study has successfully developed a MEG geothermal cement with independent intellectual property rights that provides reliable technical support for the efficient development of geothermal resources and has important engineering application value.
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Scientific Reports
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2025
·Vol. 15
·期 1
·1-13
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IF 3.9
Xia Meng,Cao Shisheng,Liu Zeqi,Zhao Jiakang,Yuan Yibo,Ni Jianhui,Guo Zhengjian,Wang Weihan,Yin Huijuan,Zou Zhaohui
Persistent or refractory apical periodontitis is primarily caused by microbial retention, as conventional root canal treatment often fails to eliminate infections completely, and systemic antibiotic therapy is insufficient to achieve effective concentrations for eradicating bacterial biofilms within root canals. This highlights the urgent need for novel therapeutics offering safe and effective antimicrobial strategies. Antimicrobial photodynamic therapy (aPDT) is a promising approach for root canal disinfection. However, commonly used photosensitizers such as Ce6 suffer from poor water solubility and strong aggregation tendencies, resulting in limited penetration into infected sites. In this study, we developed a DNase I-Lip@Ce6 nanodelivery system by combining deoxyribonuclease I (DNase I) with liposome-encapsulated Ce6. The liposomal carrier facilitated efficient delivery of Ce6 into target bacterial cells, while DNase I degraded extracellular DNA in the biofilm matrix, weakening its protective barrier. This synergistically enhanced Ce6 penetration and therapeutic efficacy, leading to the successful eradication of planktonic Enterococcus faecalis and in vitro biofilms. This strategy offers a novel approach for the precision treatment of persistent oral infections and holds strong potential for clinical translation.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2025
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IF 3.8
Pan Yalong,Han Lijun,Shen Junxue,Li Jing,Sun Taolei,Yu Yao
Pesticide contamination is a global challenge, threatening agricultural production and environmental safety. To address the urgent need for efficient and reliable pesticide detection methods, we developed a novel multi-channel colorimetric sensor array based on HAuCl 4 -mediated oxidation reactions. This innovative system incorporates six distinct colorimetric channels coupled with advanced multivariate statistical analysis, enabling simultaneous detection and discrimination of multiple pesticide residues. Through systematic optimization of key reaction parameters, the developed sensor improved selectivity and classification accuracy, particularly for structurally similar pesticides. The platform successfully identifies 11 different classes of pesticides and distinguishes five specific pesticides across various concentration levels. This approach overcomes the limitations of traditional single-target detection methods by employing multi-parameter analysis for accurate pesticide identification. Notably, the proposed method offers several practical advantages, including simple operation, rapid detection (within 30 min), high sensitivity (detection limits of 6 μg/mL), and cost-effectiveness by eliminating the need for expensive instrumentation. These features make the developed sensor array particularly suitable for on-site pesticide screening and food safety monitoring applications. Graphical
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Nature Communications
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2025
·Vol. 16
·期 1
·1-15
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IF 15.7
Zhang Chunyu,Li An-Zhen,Yuan Bo-Jun,Liu Xiang,Liu Yuanbo,Kong Kejian,Shi Qiujin,Zhang Yixuan,Luo Yiqi,Li Shengnan,Zhou Hua,Duan Haohong
H 2 S capture and valorization from polluted natural gas offer environmental and resource recovery benefits, but current approaches produce moderate-value sulfur with intensive carbon footprint. Herein, we develop an electrochemical deep oxidation method that converts H 2 S from polluted natural gas into value-added K 2 SO 4 using in-situ cathodically generated H 2 O 2 . We first validate this concept using commercial H 2 O 2 and then in-situ generated H 2 O 2 in H-cell, revealing the importance of high H 2 O 2 concentration for deep H 2 S oxidation, especially sluggish S 2 O 3 2− -to-SO 3 2− conversion. We then showcase its application potential in 4-cm 2 and then 100-cm 2 flow reactor with high interfacial H 2 O 2 concentration and large current, with the latter achieving H 2 S removal (100,000 ppm to <15 ppm), >70% K 2 SO 4 selectivity, and 100-h stable operation. Life-cycle assessment and techno-economic analysis confirm the strategy’s sustainability advantages and economic viability. We finally extend this method to produce a 1.4 wt% H 2 SO 4 solution by modifying the flow reactor with a solid-electrolyte type.
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Communications Chemistry
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2025
·Vol. 8
·期 1
·1-12
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IF 6.2
Bai Si-Cong,Wang Ye-Cheng,Ye Wei-Lin,Wu Sheng-Yen,Jia Lee,Hong Liu,Xie Huan-Zhang,Li Gao
Abnormal amyloid beta (Aβ) aggregation is a key factor in Alzheimer’s disease (AD) pathology. Despite efforts to develop Aβ aggregation inhibitors, efficient screening strategies remain limited. Here, we initially applied structure-based virtual screening to target Aβ fibril ends, aiming to identify compounds that could effectively combat AD neurodegeneration. Using this approach, we screened a traditional Chinese medicine library with thousands of compounds, and selected high-scoring hits likely to inhibit fibril elongation or promote fibril depolymerization. Forsythoside A emerged as the most potent inhibitor, effectively reducing Aβ 40 aggregation and disassembling pre-formed fibrils, as confirmed through biophysical assays and surface plasmon resonance analysis. Chemical kinetics further elucidated its inhibitory mechanism. In Caenorhabditis elegans , forsythoside A alleviated Aβ-induced toxicity and extended the lifespan of the AD transgenic models. Further virtual screening and experimental validation using a CNS-penetrant compound library confirmed the robustness of our strategy. Our study underscores the effectiveness of virtual screening in the identification of Aβ aggregation inhibitors and emphasizes the critical significance of targeting fibril ends.
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CHINESE JOURNAL OF POLYMER SCIENCE
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2025
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IF 4
Si Lu-Lu,Yang Jing-Zhi,Kong An-Nan,Song Yan,Zhang Da-Wei,Li Guo-Liang
Development of polymers with underwater self-healing and antifouling properties is crucial, particularly in harsh marine environments. In this study, polydimethylsiloxane (PDMS)-based antifouling polymers with tunable self-healing capabilities in aqueous conditions were fabricated by incorporating amphiphilic segments and Fe 3+ -catechol dynamic coordination crosslinking. The microphase formed within the PDMS matrix imparted static antifouling properties to the coatings. The mechanical properties of the damaged sample were restored at room temperature in an aqueous environment for 24 h, achieving a self-healing efficiency of almost 100%. The synthesized material exploited the dynamic coordination between Fe 3+ and catechol to facilitate underwater self-healing. No bacterial adhesion was observed at the scratch site after the coating was repaired. This material enables the long-term antifouling and autonomous repair of marine vessels and sensors, thereby reducing maintenance costs.
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ENVIRONMENTAL MONITORING AND ASSESSMENT
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2025
·Vol. 197
·期 8
·1-13
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IF 3
Luo Huili,Pan Xiaopeng,Yang Yixuan,Huang Yilu,Xiang Yuhang,Li Anxin,Zhao Jia
Bisphenol analogues (BPs), the endocrine-disrupting compounds, have been detected in water, sediment, and even agricultural products. Irrigation is the main way for material migration, and it has been suggested as one of the sources of BPs. But the attention to irrigation water and sediment, and further to the ecological risks, is rare. In a hilly irrigation area in southern China, the partition and accumulation, contribution sources, and potential ecological risk of four bisphenols (BPA, bisphenol S, F, and AF) in the irrigation canal were investigated in the spring of 2023 and 2024. All four BPs were detected in the irrigation water and sediment. BPF was the dominant of 19.6–60.9 ng/L in water, while BPA was still the prominent compound (46.2–54.4% of ∑BPs) in sediment. BPs in the irrigation canal were related to the direct discharge of aquaculture, domestic, and planting drainage. Although low risks for each compound, the comprehensive risk (∑RQ) of sediments went into medium risk. BPF and BPA were the main risk contributors. BPs in sediments increased steadily during the investigation. The special accumulative point was where slow flow maintained, especially caused by the hilly change. This comprehensive study of BPs in hilly irrigation canals should be valuable for reference in similar areas. And it will be beneficial for environmental protection and production safety.
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WATER AIR AND SOIL POLLUTION
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2025
·Vol. 236
·期 10
·1-14
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IF 3
Liu Jingliang,An Fengxia,Zheng Di,Li Chao,Sun Jingya
In this study, Mn-Ni-layered double hydroxides (Mn-Ni-LDHs) were synthesized and utilized to activate peroxymonosulfate (PMS) for degrading reactive brilliant blue KN-R (KN-R) in aqueous solutions. The characterization results demonstrate that the catalysts synthesized are layered structures, and the surface area is 169.3 m 2 /g. The Mn-Ni-LDHs exhibited significant efficiency effective for the degradation of KN-R via activating PMS, achieving 96.5% degradation of KN-R within 90 min under optimal conditions (0.20 g/L catalyst, 10 mM PMS, pH=10.0 and 25°C). The reaction followed pseudo-first-order kinetics with a rate constant of 0.0351 min -1 , and the apparent activation energy was calculated to be 11.94 kJ/mol. Electron paramagnetic resonance (EPR) and free radical scavenging experiments identified ·O 2 − and 1 O 2 as the primary reactive species involved in the reaction for KN-R degradation. The proposed catalytic mechanism suggests that redox cycling between Mn 4+ /Mn 3+ and Ni 3+ /Ni 2+ within Mn-Ni-LDHs plays a critical role in enhancing PMS activation, thereby promoting the continuous generation of active oxygen species.
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BMC Psychology
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2025
·Vol. 13
·期 1
·1-15
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IF 3
Liu Yuexuan,Hou Junlin,Qi Zhaoyao,Yang Liping,Du Zhixin,Wu Yongye,Song Qi,Li Xiaobing,Sun Jianhua,Chen Ping,Guan Xutao,Li Lingling
Objective Prenatal stress has been proven to be associated with dysbiosis of the gut microbiota. Despite the established phenomenon that psychological stress can be transmitted to offspring and the ability of maternal gut microbiota to colonize the offspring’s gut through vertical transmission, the intricate relationships linking cross-generational depression with the microbiome remain poorly understood. Methods We utilized combined fear stress stimuli to establish a pregnancy psychological stress (PPS) rat model, in which offspring exhibited trans-generational depression-like behavior. The relationship between vertical transmission of the gut microbiome, intergenerational effects, and psychological stress in offspring was investigated using microbiology and metabolomics. Results We demonstrated that the vertical transmission of co-altered species from PPS dams to their puberty offspring was strongly associated with dysbiosis of the gut microbiota in the offspring. In terms of microbial function, both PPS dams and their offspring exhibited upregulation of glycine, glutamate, and serine metabolism in fecal samples, as revealed by untargeted metabolomics. Additionally, this microbial trans-generational effect was reflected in the prefrontal cortical tissue of PPS offspring, where serine in the pathway and its interconverted glycine was significantly increased. Furthermore, the co-altered species and metabolites of the pathway formed a highly correlated module with disordered inflammatory factors and neurotransmitters in the prefrontal cortex tissue of PPS offspring. This indicates that the microbiome plays a significant role in prefrontal cortex neuroinflammation as well as neurotransmitter disorders in depression-like offspring. Conclusions Our findings highlight the gut microbiome as a plausible mediator of prenatal stress effects on offspring neurodevelopment, though further mechanistic validation is required.
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Journal of Cardiovascular Translational Research
·
2025
·
IF 2.5
Zhou Qiaofeng,Jiang Na,Li Shihuan,Li Suqin,Liu Jie,Yu Liangzhu,Liu Guoli,Xia Hongli,Li Mincai
Myocardial fibrosis (MF) severely impairs the heart structure and function post-myocardial infarction. The study investigated the effectiveness and mechanism of diammonium glycyrrhizinate (DG) on ISO-induced MF. ISO-stimulated mouse cardiac fibroblasts (CFs) were treated with DG to assess the proliferation, inflammation, and fibrosis markers (α-SMA, collagen, TGF-β1, Smad3). The MF model was induced in mice by administering ISO, followed by a 4-week treatment with DG (60 mg/kg/day). Cardiac function was measured using echocardiography, and histology and molecular analyses were performed. DG significantly suppressed the CF proliferation and reduced the expression of fibrotic markers. In ISO-treated mice, DG improved the cardiac function and attenuated the upregulated fibrosis markers. Molecular analysis revealed DG suppressed the TGF-β1/Smad3 pathway activation. The antifibrotic effect was enhanced when combined with STAT3 inhibition. DG effectively alleviates ISO-induced myocardial fibrosis dysfunction by inhibiting the STAT3/Smad3 signaling pathway, demonstrating its potential as a treatment for cardiac fibrosis. Graphical abstract
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