APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2020
·Vol. 104
·期 24
·10503-10513
·
IF 3.53
Hu Gaowei,Miao Yingjie,Luo Xi,Chu Wenhui,Fu Yongqian
Abstract Cell membranes are a great obstacle for entrance of gene therapeutic agents. Cell-penetrating peptides (CPPs) have been proven as a promising gene delivery tool. However, the early TAT peptide derived from the HIV transcription activator protein has been proven that the sequence contains Furin protease cleaved motifs which limited the TAT application in delivery of exogenous active molecules. In the present study, through the bioinformatics and experimental approach, we have identified a novel CPP derived from the N terminus of VP1 protein of chicken anemia virus (CAV), designated as CVP1-N2, which is rich in arginine residues and contains α-helical structure. Then, the ability of CVP1-N2 cell penetrating was detected using confocal imaging and flow cytometry. FITC-labeled CVP1-N2 peptide could rapidly internalize into different types of live cells with dose dependence and without cytotoxic effects by MTT assay. Surprisingly, CVP1-N2 with a pattern of nuclear sub-location has shown the higher uptake efficiency than TAT. At 10, 1, and 0.1 μM, the mean relative internalization of CVP1-N2 was respectively 1.08-, 12-, and 75-fold higher than that of CVP1, as well as 1.6-, 56-, and 75-fold higher than that of TAT. Moreover, using endocytic inhibitors along with low-temperature stress validated that the CVP1-N2 internalization route is direct translocation pathway. Finally, the capacity of CVP1-N2 for delivery of gene into cells was determined, where it was able to carry red fluorescent protein (RFP) and apoptin genes into cells respectively and induce the apoptosis. All these data indicate that CVP1-N2 could be used as a novel gene delivery vehicle for gene therapy in the future. Key points • 1CVP1-N2 was identified as a novel more efficient cell-penetrating peptide.
• 2. CVP1-N2 localized to the nucleus through the direct transduction pathway.
• 3. CVP1-N2 was able to deliver the apoptin gene into HCT116 cells and induce apoptosis.
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高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2020
·Vol. 31
·期 24
·22375-22384
·
IF 2.22
Wang Li,He Lin,Li Ji,Yu Yongsheng,Li Haibo
M-type strontium ferrite is a kind of magnetic functional material with wide application prospect. In this work, SrFe 12− x Al x O 19 (0 ≤ x ≤ 1) and Sr 1− y Ca y Fe 8 Al 4 O 19 (0 ≤ y ≤ 0.4) have been prepared by a hydrothermal method, and the effects of Al 3+ doping and Al 3+ and Ca 2+ co-doping on the magnetic properties have been investigated. X-ray diffraction analysis indicates all the samples are a hexagonal structure, and the shift of the diffraction peaks suggests that Al 3+ and Ca 2+ are successfully doped in SrFe 12 O 19 . Field emission scanning electron microscope images show the particles have the hexagonal shape with the size of 1.1–2.9 µm. Vibrating sample magnetometer reveals that the doping of Al 3+ and the co-doping Al 3+ and Ca 2+ can enhance magnetic properties of strontium ferrite. For example, the values of coercivity and the saturation magnetization of SrFe 8 Al 4 O 19 are 914.1 Oe and 44.0 emu/g, which are 3% and 117% enhancement compared with SrFe 12 O 19 , respectively. For Sr 1− y Ca y Fe 8 Al 4 O 19 , coercivity and saturation magnetization increase from 914.1 Oe and 41.8 emu/g to 1047.2 Oe and 50.2 emu/g when the Ca 2+ doping concentration increases from 0 to 0.4. Moreover, the effects of acid washing also on phase composition, morphology and magnetism are researched. The as-synthesized doping strontium ferrite will be useful for the applications in security switching and recording media.
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JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 56
·期 3
·2725-2737
·
IF 3.553
Chen Shaojun,Xie Jiapeng,Liu Jiaoshi,Huang Xiaoting,Wang Cai
Flexible, self-healing hydrogels with a variety of desirable properties are required for artificial skin applications. Here, we report a novel ionic conductive composite hydrogel consisting of polyacrylamide (PAM), a silk sericin (SS) network and NaCl for use on bionic skin.
The use of NaCl made the hydrogels good ionic conductors. Intrinsic interactions between the PAM and SS endowed the hydrogel with good mechanical properties, including a high tensile strength of 0.36 MPa and a compressive strength of 0.38 MPa. The ionic hydrogel showed a high transparency of 93%, a high adhesion strength of 52 kPa, and good conductivity. After being fractured, two pieces of the ionic hydrogel were re-bonded without external stimulation, which simulated the self-healing properties of skin after injury. The hydrogel displayed moisturizing and anti-mildew properties after being stored at room temperature for 2 months. These ionic hydrogels may find applications in artificial skin, human–machine interfaces and soft robotics.
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 8
·1-9
·
IF 6.232
Hu Xuemei,Wei Zixuan,Sun Chaoqun,Long Yijuan,Zheng Huzhi
Cu 2+ are found to greatly reduce the photoinduced oxidase activity of fluorescein and then inhibit the chromogenic reaction catalyzed by fluorescein. A simple colorimetric assay for Cu 2+ is established. Based on this, bifunctional nanocomposites of α-fetoprotein (AFP) antibody (Ab) and copper-based metal-organic framework (Ab2@Cu-MOF) are synthesized by the simple self-assembly of AFP Ab2, Cu 2+ , and 4,4′-dipyridyl: the binding site of AFP Ab2 exposed on the surface of the nanocomposites can specifically recognize AFP antigen; Cu 2+ in nanocomposites can inhibit the visible light-induced activity of fluorescein. The structure of Ab2@Cu-MOF disintegrate and Cu 2+ is released in an acetate buffer solution. The higher the amount of AFP antigens, the more significant the inhibitory effect. Thus, the Ab2@Cu-MOF immunoassay for AFP determination is established using 3,3′,5,5′-tetramethylbenzidine as chromogenic substrate with a detection limit of 35 pg.mL −1 . This simple, cheap, and sensitive method sheds substantial light on practical clinical diagnosis. Meanwhile, the mechanism of inhibition is revealed to facilitate the targeted selection of enzyme regulators.
Diagrammatic illustration of Cu 2+ detection (part a) and Ab2@Cu-MOF immunoassay for sensing α-fetoprotein based on the synthesized Ab2@Cu-MOF nanocomposites (parts a and b).
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高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 8
·1-9
·
IF 6.232
Zhang Ying,Yuan Xinyue,Jiang Wei,Liu Huilin
Quantum-dots-doped covalent organic frameworks in a molecularly imprinted network (QDs-doped COFs@MIP) were developed for detection of nereistoxin (NRT)-related insecticide in tap water. The preparation of QDs-doped COFs@MIP was easy to accomplish via one-pot synthesis at room temperature. QDs-doped COFs@MIP quenched by targeting thiosultap due to the photoinduced charge transfer. A Brunauer–Emmett–Teller surface area of 186.20 m 2 g −1 and a maximum adsorption capacity of 771 mg g −1 of the QDs-doped COFs@MIP exhibited good selectivity and adsorption capacity. Direct fluorescence determination was established over the range 5–100 μg L −1 ( R 2 = 0.9959) with a detection limit of 1.60 μg L −1 . Furthermore, 86.5–106.5% recoveries of spiked tap water were achieved. The determination system was feasible for tracing the NRT-related insecticide with high accuracy and good repeatability and reproducibility.
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高影响因子
CHEMICAL PAPERS
·
2020
·Vol. 75
·期 1
·115-122
·
IF 1.68
Gao Jie,Zhang Lingfan,Qian Junchao,Chen Feng,Zhang Wenqing
N-doped TiO 2 fiber membranes were prepared by electrospinning method and adsorptive chitosan was added as a nitrogen source. The obtained TiO 2 fiber membranes were analyzed by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, Raman spectroscopy and X-ray photoelectron spectroscopy (XPS). The results show that the film has adsorption properties; furthermore, the morphology of the TiO 2 fiber with a chitosan content of 0.5 g after calcination provides the best catalytic performance; An anatase–rutile titania was found in the oxidized fiber film found in the Raman spectrum TiO 2 ; XPS demonstrated that the introduction of chitosan could replace N atom with partial lattice oxygen in TiO 2 . Moreover, the degradation rate of methylene blue of the material was about 60–80% after 4-h reaction, and the maximum degradation rate was achieved by nanofiber film with chitosan concentration of 0.5 g.
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高影响因子
ACTA PHARMACOLOGICA SINICA
·
2020
·Vol. 42
·期 3
·393-403
·
IF 5.064
Tian Jia-hui,Wu Qian,He Yong-xiang,Shen Qi-ying,Rekep Mubarak,Zhang Gui-ping,Luo Jian-dong,Xue Qin,Liu Ying-hua
Endoplasmic reticulum stress (ER stress) plays a key role in the development of cardiac hypertrophy and diabetic cardiomyopathy (DCM). Zonisamide (ZNS) was originally developed as an antiepileptic drug. Studies have shown that ZNS suppresses ER stress-induced neuronal cell damage in the experimental models of Parkinson’s disease. Herein, we investigated whether ZNS improved DCM by attenuating ER stress-induced apoptosis. C57BL/6J mice were fed with high-fat diet (HFD) and intraperitoneally injected with low-dose streptozotocin (STZ) to induce type 2 diabetes mellitus (T2DM), and then treated with ZNS (40 mg·kg −1 ·d −1 , i.g.) for 16 weeks. We showed that ZNS administration slightly ameliorated the blood glucose levels, but significantly alleviated diabetes-induced cardiac dysfunction and hypertrophy. Furthermore, ZNS administration significantly inhibited the Bax and caspase-3 activity, upregulated Bcl-2 activity, and decreased the proportion of TUNEL-positive cells in heart tissues. We analyzed the hallmarks of ER stress in heart tissues, and revealed that ZNS administration significantly decreased the protein levels of GRP78, XBP-1s, ATF6, PERK, ATF4, and CHOP, and elevated Hrd1 protein. In high glucose (HG)-treated primary cardiomyocytes, application of ZNS (3 μM) significantly alleviated HG-induced cardiomyocyte hypertrophy and apoptosis. ZNS application also suppressed activated ER stress in HG-treated cardiomyocytes. Moreover, preapplication of the specific ER stress inducer tunicamycin (10 ng/mL) eliminated the protective effects of ZNS against HG-induced cardiac hypertrophy and ER stress-mediated apoptosis. Our findings suggest that ZNS improves the cardiac diastolic function in diabetic mice and prevents T2DM-induced cardiac hypertrophy by attenuating ER stress-mediated apoptosis.
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高影响因子
BMC BIOTECHNOLOGY
·
2021
·Vol. 21
·期 1
·1-14
·
IF 2.563
Zhang Dongliang,Shao Jialiang,Zhuang Jingming,Zhou Shukui,Yin Shuo,Wu Fuyue,Hou Jiangang,Wang Xiang
Background Human epithelial cell sheets (ECSs) are used to clinically treat epithelial conditions such as burns, corneal blindness, middle ear cholesteatoma and vitiligo. As a widely used material in clinic, there is little information on the biobanking of ECSs and its repair effect after storage.Results Two methods for biobanking foreskin ECSs were compared in a short term (7 days): 4-degree storage and programmed cryopreservation. Cell sheet integrity, viability, apoptosis, immunogenicity, mechanical properties and function were evaluated. In vivo, ECSs were directly transplanted to skin defect models and histological examination was performed at 1 week postoperatively. We successfully extracted human foreskin-derived primary epithelial cells and fabricated them into ECSs. Compared with 4-degree storage, programmed cryopreservation preserved the ECS structural integrity, enhanced the mechanical properties, decreased HLA-I expression, and increased cell viability and survival. An increased proportion of melanocytes with proliferative capacity remained in the cryopreserved sheets, and the undifferentiated epithelial cells were comparable to those of the fresh sheets. In vivo, cryopreserved ECSs could reduce inflammatory cell infiltration and promote connective tissue remodeling, epithelial cell proliferation and vascular regeneration.Conclusion sProgrammed cryopreservation of ECSs was superior and more feasible than 4-degree storage and the cryopreserved ECSs achieved satisfying skin wound healing in vivo. We anticipate that the off-the-shelf ECSs could be quickly used, such as, to repair human epithelial defect in future.Graphical abstract
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高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2021
·Vol. 23
·期 2
·1-12
·
IF 2.253
Yang Yongsheng,Gu Bingli,Liu Zhiduo,Chen Da,Zhao Yun,Guo Qinglei,Wang Gang
Doped carbon-based materials have attracted considerable attentions due to their extraordinary optical, thermal, and electronic properties. Herein, we demonstrate a facile and universal approach, which involves the hydrothermal treatment of citric acid and phosphonitrilic chloride trimer (Cl 6 N 3 P 3 ), for the production of nitrogen and phosphorus co-doped graphene quantum dots (N, P-GQDs). The obtained N, P-GQDs with a mean size of about 3.4 nm exhibit bright yellow fluorescence, good-solubility, and attractive optical stability. Although the quantum yield as high as 34.8% has been proved in our synthesized N, P-GQDs, the fluorescence can be also fleetly and selectively quenched by Fe 3+ ions. Therefore, high-performance Fe 3+ sensors are fabricated with N, P-GQDs, with an ultra-sensitive detection limit of 146 nM. Furthermore, high ionic strength, mild acids, and alkaline are demonstrated to have a small impact on the fluorescence intensity of the N, P-GQDs. Finally, the as-synthesized N, P-GQDs, with bright luminescence and excellent biocompatibility, are applied for bioimaging, e.g., fibroblast cells.
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高影响因子
Electrocatalysis
·
2020
·Vol. 11
·期 5
·513-521
·
IF 2.587
Xiao Haolin,Wei Shanshan,Chen Zhencheng,Cao Liangli
Herein we report a label-free amperometric immunosensor based on reduced graphene oxide-tetraethylene pentamine (rGO-TEPA) hybrid material co-modified by gold and iron-oxide magnetic nanoparticles (Au/MNPs-rGO-TEPA) for detection of carcinoembryonic antigen (CEA). The Au/MNPs-rGO-TEPA nanomaterials were prepared and coated on the working electrode as a sensing platform for antibody (anti-CEA) immobilization and signal amplification. This nanomaterial exhibited some excellent functionalities, such as magnetic and enhanced electrical properties due to its large specific surface area and favorable conductivity. Thionine (Thi) molecule was attached onto rGO-TEPA via π-π stacking and was used as an electrochemically active substance. The immunosensor was developed based on the increase in quantity of CEA captured, proportional to the decrease in peak currents of Thi. Under optimal conditions, the Au/MNPs-rGO-TEPA/Thi-based immunosensor showed impressive linear range from 0.1 pg/mL to 150 ng/mL. In addition, the proposed method had excellent linearity, high sensitivity, and relatively good stability, thereby offering a good platform for early clinical analysis.
A novel label-free binary nanoparticle-decorated reduced graphene oxide-tetraethylene pentamine-based electrochemical immunosensor was developed. Au/MNPs-rGO-TEPA/Thi was used as electrochemical sensitive platform for quantitative detection of CEA.
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高影响因子
FIBERS AND POLYMERS
·
2020
·Vol. 21
·期 4
·717-723
·
IF 1.797
Duan Yinli,Ma Jing,Liu Jiao,Qiang Liangsheng,Xue Juanqin
In this paper, a facile and efficient method was performed to prepare the thermo-responsive Poly(N-isopropylacrylamide)/TiO 2 (TiO 2 /PNIPAM) with the dispersing of TiO 2 nanoparticles into PNIPAM. The structures of nanoparticles and the grafted polymer were confirmed by X-ray diffraction (XRD), Raman spectra, Fourier transform infrared spectra (FT-IR) and Dynamic Light Scattering (DLS). The interactions between the two components were studied by X-ray photoelectron spectroscopy (XPS), and the morphology of TiO 2 /PNIPAM was observed by scanning electron microscopy (SEM). The results showed that TiO 2 exhibited homogeneous dispersion, without sacrificing the properties of TiO 2 . Furthermore, The composite was used to photocatalytic degradation of Methylene blue (MB). The highest MB removal efficiency is 86.3 %. The photocatalytic activities above or below the lower critical solution temperature (LCST) of PNIPAM was also investigated. The experiment results show that the existence of PNIPAM contributed not only the thermo-responsive abilities to TiO 2 , but also the enhanced photocatalytic activities to TiO 2 . In addition, the corresponding mechanism of enhanced photocatalytic activity was proposed. The results manifest that the well-designed TiO 2 /PNIPAM is a promising candidate in wastewater treatment application.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 55
·期 21
·9109-9118
·
IF 3.553
Guo Yan,He Miaomiao,Peng Yue,Zhang Qi,Yan Luke,Zan Xingjie
Due to their high mechanical properties and recoverability, hybrid double-network (DN) hydrogels composed with a physically cross-linked network (the first network) and a chemically cross-linked network (the second network) arouse researchers’ interest and attention in various fields. In this work, we have fabricated hybrid double-network hydrogels with ionically cross-linked κ-carrageenan as the first network and covalently cross-linked poly( N -acryloyl glycinamide) (PNAGA) as the second network by using an one-pot method. Because of synergistic interaction between κ-carrageenan and PNAGA polymer chains, the hybrid DN hydrogels exhibit excellent mechanical properties with a breaking stress of 1.7 MPa and a breaking strain of 250%, which are much better than their parent single-network hydrogels. In addition, due to the physical cross-links among κ-carrageenan and PNAGA, the hybrid DN hydrogels show good self-healing ability; and due to the biocompatibility of all components, the hybrid DN hydrogels show low cytotoxicity. All those properties endow the potential application of κ-carrageenan/PNAGA DN hydrogel in bioengineering.
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