JOURNAL OF APPLIED POLYMER SCIENCE
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2025
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IF 2.8
Pengtao Lei,Xiangyang Xu,Chenyu Huang,Dongsheng Chen,Zhenkun Jiang
To optimize the proton transport performance of polyvinylidene fluoride (PVdF), a proton exchange membrane (PEM) material, carbon nanotube (CNT), and zirconium hydrogen phosphate (ZrP) have been blended into the PVdF matrix. Adopting a hydrothermal synthesis route, the acidic ZrP can be better conjugated with the positively charged CNT functionalized by cetyltrimethylammonium bromide (CTAB). As visualized using electroscopes, ZrP nanocrystallines are intimately dotted and homogeneously distributed on CNT. The ZrP–PVdF interaction can be attributed to hydrogen bonding between hydroxyl and F-terminal groups. By incorporating well CNT/ZrP into PVdF via solution casting, the pore architecture can be regulated; namely, the macro-voids in PVdF are greatly reduced. The composite membranes exhibit tensile stress of 48–52 MPa while sustaining the thermal stability of PVdF. The incorporated CNT/ZrP can suppress void porosity and enhance film hydrophilicity. With improved wettability, the methanol permeability can be reduced to less than 2 × 10 −9 cm 2 ∙s −1 , ca. one-sixth that of pure PVdF. The proton conductivity for PVdF-CNT 0.03 -ZrP 0.06 , with CNT and ZrP mass content respectively 3 and 6 wt% that of PVdF, attains 0.089 S·cm −1 at 100°C, showing upgraded conducting capability. The membrane selectivity can be remarkably enhanced, verifying that CNT/ZrP blending is a facile and profitable approach for PEM optimization.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
Jing Tian,Yanfeng Zhao,Fanghang Li,Yezhu Xu,Wenting Yi,Su Jiang,Yunping Tang,Yun Lu
Rutin has attracted widespread attention due to its multiple pharmacological activities, but inherent defects such as poor water solubility and low bioavailability have greatly limited its clinical applications. Therefore, this study employed cyclodextrin glucanotransferase (CGTase) to catalyze the glycosylation modification of rutin, preparing rutin derivatives with improved water solubility. After optimizing the conditions, we isolated and purified 98.33 % of high-purity α-monoglucosyl rutin (α-GR), and its structure was also characterized. Subsequently, the cyclophosphamide (CTX)-induced murine liver injury model was used for activity evaluation. Our results showed that α-GR could significantly reduce liver function indicators (AST, ALT, and TBA), regulate antioxidant enzyme activities and inflammatory factor levels, and effectively attenuate CTX-induced liver injury. Furthermore, integrated metabolomics, transcriptomics, and RT-PCR analyses indicated that α-GR primarily exerts its hepatoprotective effects by intervening in key metabolites and gene expressions in CTX metabolic processes, related inflammatory responses, and oxidative stress pathways. Our results show that α-GR can effectively repair liver damage caused by CTX, thus laying the foundation for its use as a dietary supplement or therapeutic adjuvant.
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RSC Advances
·
2025
·Vol. 15
·期 31
·25579-25585
·
IF 4.6
Lili Jiang,Yanyan Jia,Hang Yin,Yong Liu
Pterostilbene, a dietary polyphenol in blueberries, grapes and wine, possesses an anti-diabetic efficacy. However, the mechanism of anti-diabetic efficacy for pterostilbene is poorly understood. The purpose of the present study was to systematically investigate inhibitory effects and inhibition mechanisms of pterostilbene or its metabolite (pinostilbene) against aldose reductase and α-glucosidase. In the present study, inhibition kinetics and molecular docking were applied to investigate the effect of pterostilbene and pinostilbene on the activity of aldose reductase and α-glucosidase. The results showed that pinostilbene exhibited stronger inhibition on aldose reductase (IC50 = 55.41 ± 4.31 μM) and α-glucosidase (IC50 = 11.69 ± 0.888 μM) in noncompetitive-type manner. Whereas, pterostilbene displayed the weak effect on aldose reductase and α-glucosidase (IC50 = 1569 ± 177.858 μM) inhibition. Moreover, pinostilbene interacted with amino acid residues outside of aldose reductase and α-glucosidase active site to inhibit its activities by van der Waals, π–anion or π–alkyl interactions. The current study has provided comprehensive reference not only for the anti-diabetic mechanism of pterostilbene, but also offered a basis to develop and design adjuvant antidiabetic drugs or novel functional foods.
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CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Yingpei Miao,Shuqing Yu,Qingcui Zhang,Xiaomei Li,Bowei Xia,Li Yang,Xiaobin Li,Junwei He
Leonurus japonicus Houtt. (LJ) is a pivotal traditional Chinese medicine remedy for thrombosis; however, its potential mechanisms and active constituents remain unclear. In this study, LJ exhibits a significant antithrombotic effect on an arteriovenous bypass thrombosis rat, characterized by reductions in thrombus wet and dry weights, decreased maximum aggregation rate, increased thrombus formation inhibition rate, aggregation inhibition rate, alterations in fibrinogen and antithrombin III levels, and shortened coagulation indicators. Moreover, LJ significantly enhanced the erythrocyte staining area and intensity in an arachidonic acid-induced zebrafish heart. Network pharmacology indicated that LJ alleviates thrombosis by regulating TNF and IL-17 pathways. Metabolomics revealed that LJ significantly modulated 29 endogenous differential metabolites, linking it to the regulation of primary bile acid biosynthesis, IL-17, and TNF pathways. Zebrafish experiments and molecular docking suggested that kaempferol and apigenin exhibit antithrombotic effects by modulating TNF and IL-17 proteins. Finally, molecular dynamics simulation validated that kaempferol and apigenin have strong binding capabilities with TLR4, STAT3, TNF-α, and IL-17A, and their binding free energies are −20 to −27 and −13 to −15 kcal/mol, respectively. In conclusion, LJ exerts its antithrombotic effect by regulating lipid metabolism and inhibiting TLR4/STAT3/TNF-α/IL-17A pathways, with kaempferol and apigenin identified as bio-constituents. This plant holds significant potential for development and utilization in antithrombotic therapies. Graphical This study is the first to integrate network pharmacology, rat model, metabolomics, zebrafish model, molecular docking, and MD simulation techniques to elucidate the anti-thrombotic effect and mechanism of Leonurus japonicus by modulating lipid metabolism and inhibiting TLR4/STAT3/TNF-α/IL-17A pathways, with kaempferol and apigenin identified as primary pharmacological constituents.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Xinrui Liu,Yupeng Li,Dairong Chen,Xiuling Jiao,Yuguo Xia
The selective hydrogenation of α,β-unsaturated aldehydes to unsaturated alcohols is a cornerstone of fine chemical synthesis, yet it remains a formidable challenge due to competing hydrogenation of thermodynamically favored C C bonds over kinetically sluggish C O bonds. Here, we present a PtFe dual-atom catalyst anchored on defective polymeric carbon nitride (DPCN), which achieves nearly complete conversion and exceptional selectivity toward cinnamyl alcohol (COL) under mild conditions. Particularly, the PtFe/DPCN exhibits a superior turnover frequency and achieves 99.02 % conversion of cinnamaldehyde (CAL) with 99.34 % selectivity for COL at 70 °C and 2 MPa H 2 . Kinetic studies and in-situ DRIFTS analyses reveal that this dual-atom architecture facilitates preferential C O adsorption and hydrogenation while suppressing C C activation. In addition, DFT calculations further show that the Pt-Fe dual atomic pair lowers the activation barrier by modulating the d -band center and Gibbs free energy profiles, enhancing H 2 splitting and intermediate stabilization. This work demonstrates a promising strategy for fine-tuning electronic and geometric structures via dual-atom design, offering an effective route for developing highly selective hydrogenation catalysts under industrially viable conditions.
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JOURNAL OF SEPARATION SCIENCE
·
2025
·Vol. 48
·期 5
·e70169
·
IF 2.8
Bing Li,Yidan Lun,Kun Yao,Cheng Tang
In this work, for the first time, a deep eutectic solvent assisted ligand affinity assay was proposed. Several critical parameters affecting the analysis performance were investigated and the optimized DES extract conditions were as follows: the solution of tetrabutylammonium chloride–ethylene glycol (1:2) was prepared as an extract solution. A total of 1.4 mol/L sodium citrate (pH = 6.6) was used for phase separation, and the vortex extraction time was 5 min. Analytes could be enriched in the phase of deep eutectic solvent after phase separation. Then, they could be analyzed by α-glucosidase immobilized magnetic beads affinity assay directly. Results showed the proposed method could detect luteolin (a positive control) from plasma selectively. The LOD was 2 µg/mL. The intraday precision and the interday precision were 7.86% and 6.66%, respectively. By using the proposed method as a tool, plasma of the rats that administrated the extract of Pueraria lobata was analyzed. The bio-active flavonoids absorbed in the body such as puerarin, daidzin, daidzein, and a metabolite of puerarin (puerarin glucuronide) were found. The method has the benefits of a simplified analytic process, high resolution, and is a reliable method.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Haozhang Xu,Guoan Xi,Wei Chen,Yin Hu,Jun Xia,Weiguo Song,Changyan Cao,Yanhong Zeng,Dan Zhou
The development of hydrogenation catalysts with high performance and stability could decrease the byproducts and simplify the follow-up process in the industrial process. Optimizing supported catalysts through support improvement is a feasible approach to balance effectiveness and cost. In view of this, cubic perovskite NaNbO3 was modified with organosiloxane to form a heterophase junction support consisting of amorphous silica and NaNbO3 crystal. On the basis of this, the supported Ru catalyst displayed superior performance for α-pinene hydrogenation with almost complete conversion, cis-pinane selectivity, and good stability. The relationship between the structure and performance of the catalyst was systematically investigated by comprehensive experiments and multiple characterization technologies. Due to the formation of the heterophase junction, the surface hydrophobicity, specific surface area, and electron properties of the catalyst were optimized, leading to the good dispersity of the catalyst in the hydrogenation mixture and excellent adsorption and charge transfer ability, which should account for the unique performance of the catalyst, as confirmed by a series of experiments, characterizations, and DFT calculations. This work proposes an effective approach to optimize the surface structure and electronic properties of the catalysts through constructing crystallized and amorphous heterophase junction support, which is theoretically significant for the design and development of cost-effective heterogeneous hydrogenation catalysts.
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Acta Biomaterialia
·
2016
·
IF 6.008
Qianming Lin,Yumeng Yang,Qian Hu,Zhong Guo,Tao Liu,Jiake Xu,Jianping Wu,Thomas Brett Kirk,Dong Ma,Wei Xue
Hydrogels have attracted much attention in cancer therapy and tissue engineering due to their sustained gene delivery ability. To obtain an injectable and high-efficiency gene delivery hydrogel, methoxypolyethylene glycol (MPEG) was used to conjugate with the arginine-functionalized poly( l -lysine) dendron (PLLD-Arg) by click reaction, and then the synthesized MPEG-PLLD-Arg interacted with α-cyclodextrin (α-CD) to form the supramolecular hydrogel by the host-guest interaction. The gelation dynamics, hydrogel strength and shear viscosity could be modulated by α-CD content in the hydrogel. MPEG-PLLD-Arg was confirmed to bind and deliver gene effectively, and its gene transfection efficiency was significantly higher than PEI-25k under its optimized condition. After gelation, MMP-9 shRNA plasmid (pMMP-9) could be encapsulated into the hydrogel matrix in situ and be released from the hydrogels sustainedly, as the release rate was dependent on α-CD content. The released MPEG-PLLD-Arg/pMMP-9 complex still showed better transfection efficiency than PEI-25k and induced sustained tumor cell apoptosis. Also, in vivo assays indicated that this pMMP-9-loaded supramolecular hydrogel could result in the sustained tumor growth inhibition meanwhile showed good biocompatibility. As an injectable, sustained and high-efficiency gene delivery system , this supramolecular hydrogel is a promising candidate for long-term gene therapy. Statement of Significance To realize the sustained gene delivery for gene therapy, a supramolecular hydrogel with high-efficiency gene delivery ability was prepared through the host-guest interaction between α-cyclodextrin and PEGylated arginine-functionalized poly( l -lysine) dendron. The obtained hydrogel was injectable and biocompatible with adjustable physicochemical property. More importantly, the hydrogel showed the high-efficiency and sustained gene transfection to our used cells, better than PEI-25k. The supramolecular hydrogel resulted in the sustained tumor growth inhibition meanwhile keep good biocompatibility. As an injectable, sustained and high-efficiency gene delivery system, this supramolecular hydrogel is a promising candidate in long-term gene therapy and tissue engineering.
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CNS Neuroscience & Therapeutics
·
2025
·Vol. 31
·期 5
·e70437
·
IF 5
Hai‐Qian Zhang,Yan Wang,Xiao Geng,Mingxin Dong,Ziwei Liu,Chengbiao Sun,Kaikai Yu,Wenwen Xin,Ye Xu,Na Xu,Wensen Liu
ANG‐modified liposomes encapsulating α‐melittin and resveratrol increase their blood–brain barrier penetration and GB cell selectivity. The combined treatment of α‐melittin with resveratrol markedly facilitates apoptosis and pyroptosis, and promotes the apoptosis–pyroptosis switch in the GB cells via downregulating the Wnt/β‐catenin signaling.
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JOURNAL OF FOOD BIOCHEMISTRY
·
2022
·Vol. 46
·期 10
·e14280
·
IF 3.654
Tian Liang,Jiawei Hu,He Song,Lili Xiong,Yanping Li,Yang Zhou,Lifang Mao,Jiamin Tian,Huan Yan,Ersheng Gong,Jiawen Fei,Yuan Sun,Hanyue Zhang,Xiaoyin Wang
This study aimed at comparing the physicochemical characteristics, α-glucosidase inhibitory effect, and hypoglycemic activity of pectins (N-NOP and H-NOP) from peels of normal and Huanglongbing (HLB)-infected Navel oranges. Results indicated the pectins were high methoxy pectins mainly composed of homogalacturonan and rhamnogalacturonan-I. The pectins exhibited similar functional groups, surface morphology, and particle size, and had no triple-helical conformation in solution. They exerted fat and glucose absorption capacities and were mixed-type noncompetitive α-glucosidase inhibitors with IC 50 values of 1.182 and 2.524 mg/ml, respectively. Both N-NOP and H-NOP showed hypoglycemic activity in alloxan-induced diabetic mice. Administration of them could promote the synthesis of hepatic glycogen and/or serum insulin to lower blood glucose levels and enhance antioxidant status to alleviate oxidative stress injury in diabetic mice. Moreover, N-NOP had higher yield, molecular weight, ζ-potential, oil holding capacity, α-glucosidase inhibitory effect and in vivo hypoglycemic activity, whereas H-NOP possessed higher uronic acid, degree of esterification, thermal stability, water holding capacity, swelling capacity, and fat absorption capacity. It could be concluded that some similarities and differences existed between N-NOP and H-NOP in physicochemical characteristics, functional properties, α-glucosidase inhibitory effects, and hypoglycemic activity. This study provides references for the basic research and application of pectins from peels of normal and HLB-infected Navel oranges. Practical applications Pectin has been widely used in the food and pharmaceutical industries for several decades due to its health benefit, gelling, thickening, and emulsification performances. Diabetes mellitus is a worldwide concern in recent years. Pectins (N-NOP and H-NOP) from peels of normal and Huanglongbing (HLB)-infected Navel oranges possessed in vitro and in vivo hypoglycemic activities, indicating they were potential anti-antidiabetic substitutes of chemical drugs. Moreover, comparative understanding on the physicochemical characteristic, α-glucosidase inhibitory effect and hypoglycemic activity of pectins from peels of normal and Huanglongbing-infected Navel oranges was conducive to the recycling and utilization of Navel orange peels. Recently, the biological activity of pectin from peels of normal Navel oranges has been rarely reported, and the information on pectin from peels of Huanglongbing-infected Navel orange is rare. This study provides references for the basic research and application of pectins from peels of normal and HLB-infected Navel oranges.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2014
·
IF 2.711
Xiaomin Xia,Chuanyang Su,Juanli Fu,Pu Zhang,Xiaoji Jiang,Demei Xu,Lihua Hu,Erqun Song,Yang Song
This study investigated the protective effect of α-lipoic acid (LA) on lipopolysaccharide (LPS)/ d -galactosamine ( d -GalN)-induced fulminant hepatic failure in mice. First, we found that LA markedly reduced LPS/ d -GalN-induced increases in serum ALT and AST activities, which were supplemented with histopathological examination, suggested that LA has a protective effect on this model of hepatic damage. Livers challenged with LPS/ d -GalN exhibited extensive areas of vacuolization with the disappearance of nuclei and the loss of hepatic architecture. On the contrary, these pathological alterations were ameliorated by LA treatment. Next, we found that ROS and TBARS levels were increased in LPS/ d -GalN treated liver homogenates , which were attenuated by LA administration. Consistently, decreases in hepatic CAT and GPx activities were observed in LPS/ d -GalN group and were significantly restored by LA administration. Moreover, pretreatment with LA markedly reduced LPS/ d -GalN-induced iNOS , COX-2, TNF-α, NF-κB, IL-1β and IL-6 expressions. Furthermore, our data showed that TUNEL-positive cells increased in LPS/ d -GalN-treated mice liver which was counteracted by LA administration. LPS/ d -GalN induced apoptosis of hepatocytes, as estimated by caspase 3, caspase 8 and caspase 9 activations. Also, the increasing of Bax and the decreasing of Bcl-2 expressions also supported LPS/ d -GalN induced apoptosis. Interestingly, LA marked relieved these apoptotic features. Taking together, our results indicated that LA plays an important role on LPS/ d -GalN-induced fulminant hepatic failure through its antioxidant, anti-inflammatory and anti-apoptotic activities.
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Chinese Medicine
·
2019
·Vol. 14
·期 1
·1-11
·
IF 2.265
Guo Shangxin,Lin Xiaotong,Wang Yi,Gong Xingchu
Background The discovery of bioactive compounds in traditional Chinese medicine (TCM) has become an important field in TCM modernization. Ligand fishing is a suitable method for discovery of bioactive compounds in complex mixtures such as TCM with high selectivity. Because of unique advantage of low cost and convenience, paper-based microdevices can be good carriers for enzyme immobilized ligand fishing. Methods As an important enzyme for glucose metabolism, α-glucosidase was immobilized on polycaprolactone–chitosan-modified paper to prepare the microdevice with unique microfluid structure generated by 3D printing technology, which can be easily applied to screen active compounds in herbal extracts. The preparation conditions of the paper microarray were optimized. The activity of immobilized α-glucosidase was verified by colorimetric reactions which can be easily monitored by cellphone. The paper microarray with α-glucosidase immobilized was used to screen active compounds in the water extracts of mulberry leaves and lotus leaves. Results Several key parameters including Na 2 CO 3 solution concentration, Na 2 CO 3 solution volume, glutaraldehyde concentration, crosslinking time of glutaraldehyde and time of α-glucosidase immobilization were optimized. The proposed paper-based microarray was successfully applied in screening active compounds in two herbal extracts. Four compounds including chlorogenic acid, quercetin-3- O -glucuronide, isoquercetin, and quercetin were identified as α-glucosidase inhibitors. The compounds with significant non-specific adsorption caused by chitosan, such as isoquercitrin, astragalin, quercetin, were also found to be active compounds. Conclusions An enzyme immobilized paper microarray was designed and fabricated in this work. Polycaprolactone and chitosan were used to modify filter paper to prepare paper microarrays. Parameters of paper device preparation were optimized. Our findings suggested that 3D-printing paper-based microarrays can be a simple and low-cost approach for discovery of active compounds of TCM.
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