ACTA HISTOCHEMICA
·
2019
·
IF 1.719
Cui Ying,Liang Ying,Liu Yanxia,Wang Le,Cao Lili
As a neurological disorder, epilepsy has affected over 65 million people all over the world because of the unforeseeable seizures it might cause. However, in-depth understandings of the pathogenesis of epilepsy and effective treatments for the disease are still lacked. Recent discoveries suggest that autophagy, as an endogenous self-cleansing pathway in mammals, might be involved in the onset of epilepsy. Our study assumes that a non-histone DNA binding protein, high mobility group box-1 (HMGB1), formerly considered as a crucial inflammatory factor, may mediate the autophagy of neurons in epileptic mouse brain. To verify this hypothesis, pilocarpine induced epilepsy mouse model was constructed. The mice were treated with HMGB1 antibody for 4 weeks after the initial epileptic seizure . Behavioral test results suggested a recovery of learning ability and memory in epileptic mice when treated with HMGB1 antibody. Pathological changes in hippocampus were inspected under microscopes and hippocampus damages caused by seizures in mouse with epilepsy such as increased intracellular space were alleviated by HMGB1 antibody treatment. Moreover, the expressions of the proteins involved in autophagy pathways were detected by immunofluorescence staining and western blot. microtubule-associated protein 1A/1B-light chain 3 (LC3), Beclin 1, autophagy protein-5 (ATG5), and ATG7 levels were significantly decreased by HMGB1 antibody while the level of p62 was increased. TdT-mediated dUTP Nick-End Labeling (TUNEL) illustrated that cell apoptosis induced by seizures in hippocampus was mitigated by HMGB1 antibody. In conclusion, we propose that HMGB1 may induce increased autophagy in epilepsy mouse model.
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高影响因子
FREE RADICAL BIOLOGY AND MEDICINE
·
2021
·
IF 7.376
Xiulin Yang,An Chen,Qingchun Liang,Qianqian Dong,Mingwei Fu,Xiaoyu Liu,Siyi Wang,Yining Li,Yuanzhi Ye,Zirong Lan,Jing-Song Ou,Lihe Lu,Jianyun Yan
Vascular calcification is very commonly observed in patients with chronic kidney disease (CKD), but there is no efficient therapy available. Oxidative stress plays critical roles in the progression of vascular calcification. Celastrol (Cel), a natural constituent derived from Chinese herbals, exhibits anti-oxidative stress activity. Here, we investigated the effect of celastrol on vascular calcification using vascular smooth muscle cells (VSMCs), arterial rings and CKD rats. Alizarin red staining and gene expression analysis showed that Cel dose-dependently inhibited rat VSMC calcification and osteogenic differentiation. Similarly, ex vivo study revealed that Cel inhibited calcification of rat and human arterial rings. In addition, micro-computed tomography, alizarin red staining and calcium content analysis confirmed that Cel inhibited aortic calcification in CKD rats. Interestingly, Cel treatment increased the mRNA and protein levels of heme oxygenase-1 (HMOX-1), and reduced the levels of reactive oxygen species (ROS) in VSMCs. Furthermore, both pharmacological inhibition of HMOX-1 and knockdown of HMOX-1 by siRNA independently counteracted the inhibitory effect of Cel on vascular calcification. Moreover, knockdown of HMOX-1 prevented Cel treatment-mediated reduction in ROS levels. Finally, Cel treatment reduced Vitamin D3-induced aortic calcification in mice and this effect was blocked by HMOX-1 inhibitor ZnPP9. Collectively, our results suggest that up-regulation of HMOX-1 is required for the inhibitory effect of Cel on vascular calcification. Modulation of HMOX-1 may provide a novel strategy for the treatment of vascular calcification in CKD.
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高影响因子
IONICS
·
2016
·Vol. 22
·期 10
·1947-1955
·
IF 2.119
Zhuo Kelei,Ma Xueli,Chen Yujuan,Wang Congyue,Li Aoqi,Yan Changling
The molecular imprinting technique is powerful to prepare functional materials with molecular recognition properties. In this work, a potentiometric sensor was fabricated by dispersing molecularly imprinted polymers (MIPs) into plasticized PVC matrix and used for the determination of 1-hexyl-3-methylimidazolium cation ([C 6 mim] + ) in aqueous solution. The MIPs were synthesized by precipitation polymerization using 1-hexyl-3-methylimidazolium chloride ([C 6 mim]Cl) as the template molecule, methacrylic acid (MAA) and ethylene glycol dimethacrylat (EGDMA) as the functional monomers, and EGDMA also as the cross-linking agent. The as-prepared electrode exhibited a Nernstian response (58.87 ± 0.3 mV per decade) to [C 6 mim] + in a concentration range from 1.0 × 10 −6 to 0.1 mol kg −1 with a low detection limit of 2.8 × 10 −7 mol kg −1 , high selectivity, and little pH influence. The as-prepared electrode was used for the detection of the [C 6 mim] + in distilled water, tap water, and river water with a good recovery. It was also successfully applied in the determination of mean activity coefficients of [C 6 mim]Br in fructose + water systems based on the potentiometric method at 298.15 K.
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高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2020
·
IF 7.183
Yingying Liu,Wentao Yan,Zhi Wang,Huihui Wang,Song Zhao,Jixiao Wang,Peng Zhang,Xingzhong Cao
A novel aqueous solution additive, 1-methylimidazole (1-MI), was applied to prepare aromatic polyamide (PA) reverse osmosis (RO) membranes with excellent performance by interfacial polymerization of trimesoyl chloride (TMC) and m-phenylenediamine. The RO membrane structure and performance were characterized in detail. The results indicated that the addition of 1-MI was beneficial to forming the membrane with a dense and thin PA layer. This is because 1-MI could react with TMC, which is in favor of the formation of back surface structure with more pores. The product generated by 1-MI and TMC was hydrolyzed easily, and then endowed the membrane with more carboxylic acid groups. The membrane desalination performance for feeds with different NaCl concentration was evaluated. The membrane prepared with 1-MI possessed a higher flux and rejection because of the thinner and denser PA layer and more carboxylic acid groups. Considering that the membranes inevitably face chemical cleaning with acid or alkaline solutions in the real processes, the RO membranes were exposed to acidic-alkaline solution, and the results showed that the membrane prepared with 1-MI had good pH stability. This study offers a new route for preparing an excellent-performance RO membrane via using 1-MI as an additive.
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高影响因子
Autophagy
·
2024
·
IF 14.6
Juan Yi,He-Ling Wang,Guang Lu,Hailong Zhang,Lina Wang,Zhen-Yu Li,Liming Wang,Yihua Wu,Dajing Xia,Evandro F. Fang,Han-Ming Shen
Spautin-1 is a well-known macroautophagy/autophagy inhibitor via suppressing the deubiquitinases USP10 and USP13 and promoting the degradation of the PIK3C3/VPS34-BECN1 complex, while its effect on selective autophagy remains poorly understood. Mitophagy is a selective form of autophagy for removal of damaged and superfluous mitochondria via the autophagy-lysosome pathway. Here, we report a surprising discovery that, while spautin-1 remains as an effective autophagy inhibitor, it promotes PINK1-PRKN-dependent mitophagy induced by mitochondrial damage agents. Mechanistically, spautin-1 facilitates the stabilization and activation of the full-length PINK1 at the outer mitochondrial membrane (OMM) via binding to components of the TOMM complex (TOMM70 and TOMM20), leading to the disruption of the mitochondrial import of PINK1 and prevention of PARL-mediated PINK1 cleavage. Moreover, spautin-1 induces neuronal mitophagy in Caenorhabditis elegans (C. elegans) in a PINK-1-PDR-1-dependent manner. Functionally, spautin-1 is capable of improving associative learning capability in an Alzheimer disease (AD) C. elegans model. In summary, we report a novel function of spautin-1 in promoting mitophagy via the PINK1-PRKN pathway. As deficiency of mitophagy is closely implicated in the pathogenesis of neurodegenerative disorders, the pro-mitophagy function of spautin-1 might suggest its therapeutic potential in neurodegenerative disorders such as AD.Abbreviations: AD, Alzheimer disease; ATG, autophagy related; BafA1, bafilomycin A1; CALCOCO2/NDP52, calcium binding and coiled-coil domain 2; CCCP, carbonyl cyanide m-chlorophenyl hydrazone; COX4/COX IV, cytochrome c oxidase subunit 4; EBSS, Earle’s balanced salt; ECAR, extracellular acidification rate; GFP, green fluorescent protein; IA, isoamyl alcohol; IMM, inner mitochondrial membrane; MAP1LC3/LC3, microtubule associated protein 1 light chain 3; MMP, mitochondrial membrane potential; mtDNA, mitochondrial DNA; nDNA, nuclear DNA; O/A, oligomycin-antimycin; OCR, oxygen consumption rate; OMM, outer mitochondrial membrane; OPTN, optineurin; PARL, presenilin associated rhomboid like; PINK1, PTEN induced kinase 1; PRKN, parkin RBR E3 ubiquitin protein ligase; p-Ser65-Ub, phosphorylation of Ub at Ser65; TIMM23, translocase of inner mitochondrial membrane 23; TOMM, translocase of outer mitochondrial membrane; USP10, ubiquitin specific peptidase 10; USP13, ubiquitin specific peptidase 13; VAL, valinomycin; YFP, yellow fluorescent protein.
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高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Xiaohan Jin,Jingbao Wang,Siyuan Zheng,Jianan Li,Xuemei Ma,Li Feng,Hailin Zhu,Zhiyong Hu
Three triazine-based surfactants with different hydrophobic chain lengths were synthesized and characterized, namely, 2-(n-Hexylamino)-4,6-bis (3-N, N-dimethylaminopropyl) amino-1,3,5-triazine, 2-(n-Octylamino)-4,6-bis (3-N, N-dimethylaminopropyl) amino-1,3,5-triazine and 2-(n-n-Dodecylamino)-4,6-bis (3-N, N-dimethylaminopropyl) amino-1,3,5-triazine, labelled C 6 , C 8 and C 12 respectively. The chemical structure was confirmed by FTIR and 1 H NMR. The surface activity was evaluated by surface tension. C 12 had a lower critical micelle concentration (CMC) value (0.5 mM) than C 6 and C 8 , which suggested that a lower concentration was required to reach interfacial adsorption saturation, representing better inhibition performance. The corrosion inhibition of surfactants for 45 # carbon steel in 1.0 M HCl was studied by static weight loss measurement, electrochemical impedance spectroscopy (EIS), and potentiodynamic polarization. Inhibition results showed that C 12 was better than C 6 and C 8 with the maximum inhibition efficiency of 96% at 0.1 mM. The surface analysis had given a clear view of the mechanism of surfactants in inhibiting the corrosion process. Moreover, the Freundlich adsorption isotherm was the best description for the adsorption process of surfactants on 45 # carbon steel in 1.0 M HCl.
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高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2021
·Vol. 35
·期 4
·e6147
·
IF 4.105
Sujuan Ge,Yuming Zhou,Xiaoli Sheng,Chunfeng Mao,Lina Zhao,Jianxing Xu,Lei Qiu
Piperidinium-based ionic liquids (ILs) were synthesized under mild conditions by coordinating N -alkyl- N -methylpiperidinium bromide ([Pip 1,x ]Br) with anhydrous AlCl 3 . The weak basicity of N -methylpiperidine had little impact on downstream problems from proton impurities and catalyst poisoning. The results of Fourier transform infrared (FT-IR), electrospray ionization mass spectrometry (ESI-MS), and nuclear magnetic resonance ( 27 Al NMR) confirmed that the active components in [Pip 1,x ]Br-2AlCl 3 ( χ = 0.67) were [Al 3 Cl 9 Br] − and [Al 2 Cl 6 Br] − . Besides, the reaction performance of [Pip 1,8 ]Br-2AlCl 3 under different conditions was carried out by single-factor experiment and further confirmed by orthogonal experimental method. Among these reaction conditions, the optimal combination was the reaction temperature of 40°C, ILs/O molar ratio of 0.2:1, px/O molar ratio of 12:1, and the reaction time of 30 min. Furthermore, [Pip 1,8 ]Br-2AlCl 3 had excellent stability and can be recycled 14 times. It was the high-efficiency catalytic activity and prominent recyclability that made continuous industrial production possible.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2011
·
IF 3.074
Ting Wu,Xiang Cai,Shaozao Tan,Hongye Li,Jiesheng Liu,Weidong Yang
In order to explore the potential of graphene (G) in removal of hazardous chemicals in waste water, chemically reduced graphene oxide was employed as the adsorbent and the adsorption characteristics of graphene toward acrylonitrile (AN), p-toluenesulfonic acid (p-TA), 1-naphthalenesulfonic acid (1-NA) and methyl blue (MB) were evaluated under laboratory conditions. The results showed that the organic chemicals with larger molecule size and more benzene rings possessed a higher adsorption speed and a higher maximum adsorption capacity on G. The maximum adsorption capacities of p-TA, 1-NA and MB on G are ∼1.43 g/g G, ∼1.46 g/g G and ∼1.52 g/g G at 30 °C, respectively, which are the highest values of p-TA, 1-NA or MB adsorption on different kinds of today's nanomaterials. Under the maximum adsorption capacity, the absorption quantity of MB on G was dependent on the initial concentration of MB, and higher temperature could facilitate the adsorption process. The G efficiency remains almost constant during the first five cycles of the adsorption–desorption process. In addition, the fluorescence spectra implied that the adsorption of MB on G was a π–π stacking adsorption process. In conclusion, the G could serve as a promising adsorbent for the removal of chemicals containing benzene rings in waste water.
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高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2019
·
IF 4.175
Lili Ke,Xiaoyan Gan,Wenhui Zhao,Liling Guo,Hanxing Liu
At present, perovskite solar cells using two-dimensional perovskite hybrids have been researching, for their good optical performance and humidity stability work. However, there is still a great obstacle obtaining two-dimensional perovskite films of high quality and high performances. In this work, we succeeded in fabricating dense and highly crystallized Q-2D (C 6 H 5 C 2 H 4 NH 3 ) 2 FA n-1 Pb n I 3n+1 by adopting a novel two-step solution deposition method, from which a stoichiometric precursor containing PEAI and PbI 2 was first spin-coated and FAI solution was then dropped and spin-coated on the substrate. It was found that the amount of FAI solution dropped onto the substrate during the second step had great influence on the microstructure and performance of the Q-2D (C 6 H 5 C 2 H 4 NH 3 ) 2 FA n-1 Pb n I 3n+1 . Our first–generation Q-2D (C 6 H 5 C 2 H 4 NH 3 ) 2 FA n-1 Pb n I 3n+1 perovskite solar cells were higher than its 3D perovskite counterpart FAPbI 3 in moisture stability. We finally obtained Q-2D (C 6 H 5 C 2 H 4 NH 3 ) 2 FA n-1 Pb n I 3n+1 perovskite solar cells, showing an open-circuit voltage of 0.826 V and a power conversion efficiency of 11.46%.
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高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Wei Chen,Jiayue Dong,Sihan Zhou,Chunyong Zhang,Degang Fu
1-naphthol (1-NAP) and 2-naphthol (2-NAP) are both hazardous pollutants which pose great threat to our living environment. In the current work, a comparative investigation on the electrochemical mineralization of 1-NAP and 2-NAP using boron-doped diamond (BDD) anode was presented. Experiments were carried out using synthetic 1-NAP or 2-NAP solutions containing Na 2 SO 4 as supporting electrolyte . A two-level factorial design methodology was adopted to optimize the mineralization process, six mathematical models were thus constructed and used to explore the structure-property relation involved. The results obtained suggested that 1-NAP was easier and faster to break down than 2-NPA under the same oxidation conditions, which were in line with the outcome obtained from cyclic voltammetry , HPLC and TOC measurements. To understand the destruction mechanism, the electron structures of 1-NAP and 2-NAP were further examined by the quantum chemistry calculations with density functional theory. It was also found that the results from frontier orbital analysis could well explain the differing mineralization performances recorded in BDD anode cell. Finally, with the aid of LC/MS analysis, the oxidation pathways of 1-NAP or 2-NAP on BDD anodes were proposed.
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高影响因子
CATALYSIS TODAY
·
2019
·
IF 4.888
Hongxia Chen,Runduo Zhang,Wenjing Bao,Hao Wang,Zhoujun Wang,Ying Wei
The catalytic removal of HCHO with low noble-metal loading at room temperature is meaningful and challenging work. Herein, high performance catalysts x Pt/TS-1 (Si/Ti = 30, x = 0.1, 0.3, and 0.6 wt%) for HCHO oxidation were synthesized by polyvinyl alcohol-protected NaBH 4 reduction method. The Pt/TS-1 sample loading with 0.3 wt% Pt nanoparticles (NPs) could completely remove 120 ppm HCHO at space velocity (SV) of 60,000 mL/(g h) at 20 °C. Moreover, the 0.3Pt/TS-1 sample remained outstanding activity and stability in humid air with 35–75% water vapor and at high SV with a range between 60,000 and 240,000 mL/(g h). Based on the results of UV–vis, Raman, NH 3 -TPD, Contact angle measurement, CO chemisorption , and O 2 -TPD, the excellent catalytic performance of Pt/TS-1 was mostly attributed to high Pt dispersion and high concentration of adsorbed oxygen species. This work expands the field of utilization of zeolite supported noble-metal catalysts for the purification of indoor pollutants.
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高影响因子
MICROBIOLOGICAL RESEARCH
·
2016
·
IF 2.723
Waseem Raza,Ning Ling,Dongyang Liu,Zhong Wei,Qiwei Huang,Qirong Shen
The volatile organic compounds (VOCs) produced by soil microbes have a significant role in the control of plant diseases and plant growth promotion. In this study, we examined the effect of VOCs produced by Pseudomonas fluorescens strain WR-1 on the growth and virulence traits of tomato wilt pathogen Ralstonia solanacearum . The VOCs produced by P. fluorescens WR-1 exhibited concentration dependent bacteriostatic effect on the growth of R. solanacearum on agar medium and in infested soil. The VOCs of P. fluorescens WR-1 also significantly inhibited the virulence traits of R. solanacearum . The proteomics analysis showed that the VOCs of P. fluorescens WR-1 downregulated cellular proteins of R. solanacearum related to the antioxidant activity, virulence, inclusion body proteins, carbohydrate and amino acid synthesis and metabolism, protein folding and translation, methylation and energy transfer, while the proteins involved in the ABC transporter system, detoxification of aldehydes and ketones, protein folding and translation were upregulated. This study revealed the significance of VOCs of P. fluorescens WR-1 to control the tomato wilt pathogen R. solanacearum . Investigation of the modes of action of biocontrol agents is important to better comprehend the interactions mediated by VOCs in nature to design better control strategies for plant pathogens.
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高影响因子
危险品化学品经营许可证（带存储）