查找使用阿拉丁产品的科研文献

The effect of B-type procyanidin on free radical and metal ion induced β-lactoglobulin glyco-oxidation via mass spectrometry and interaction analysis

Ling Liu,Qi Dong,Youmeng Kong,Yiru Kong,Ziyin Yu,Bin Li,Haixia Yan,Xu Chen,Yixiao Shen

Procyanidin is a group of dietary flavonoids abundant in berry fruits. In this study, the effects and underlying mechanisms of B type procyanidin (PC) on free radical and metal ion (H 2 O 2 , AAPH and Fe 3+ ) induced milk protein β-lactoglobulin (BLG) glyco-oxidation were investigated. The results indicated that PC protected BLG structure changes from cross-link and aggregation induced by free radicals and metal ion. Additionally, it effectively inhibited BLG oxidation by reducing approximately 21%-30% carbonyls and 15%-61% schiff base crosslink formation. Also, PC suppressed BLG glycation by inhibiting 48–70% advanced glycation end-products (AGEs) and reduced the accumulation of intermediate product methylglyoxal (MGO). The corresponding mechanisms were elucidated that PC exhibited great free radical scavenging and metal chelating properties; PC had non-covalent bind with the amino acid residues (preferably lysine and arginine) of BLG and blocked them from glycation; PC interrupted BLG glycation by forming procyanidin-MGO conjugates. Therefore, B type procyanidin was an effective glyco-oxidation inhibitor in milk products.

Behaviour of cell penetrating peptide TAT-modified liposomes loaded with salvianolic acid B on the migration, proliferation, and survival of human skin fibroblasts

Jun Shi,Siyi Guo,Yanting Wu,Guitian Chen,Jianhui Lai,Xiaoqi Xu

Oxygen vacancy-mediated efficient electron-hole separation for C-N-S-tridoped single crystal black TiO2(B) nanorods as visible-light-driven photocatalysts

Ning Wan,Zipeng Xing,Junyan Kuang,Zhenzi Li,Junwei Yin,Qi Zhu,Wei Zhou

In-situ oxygen vacancy-mediated C-N-S-tridoped single crystal black TiO 2 (B) nanorods are smoothly manufactured. The sample preparation is divided into two steps, first of all, a simple hydrothermal process, followed by an in-situ solid-state chemical substitution method with calcination at 500 °C. We have a number of representations of the catalyst such as crystallinity, morphological structure, etc. by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and UV–vis diffuse reflectance spectroscopy, respectively. This b-C-N-S-TiO 2 (B) has the advantages of Oxygen Vacancy, single crystal structure of nanorods, Ti 3+ self-doping, C-N-S-tridoped, so this sample band gap is narrow. Moreover, through the reduction of phenol and Cr(VI), we know the photocatalytic performance of the b-C-N-S-TiO 2 (B), the reduction efficiency of phenol and Cr(VI) is ∼6.1 times and ∼7 times of no doping samples, respectively, under visible light illumination. The predominant photocatalytic performances because of the synergetic action of Ti 3+ self-doping, C-N-S-tridoped, oxygen vacancy, and the 1D single crystal B phase nanostructure, favoring the separation and transportation of photogenerated charge carriers and the visible light utilization.

Rapid and interference-free analysis of nine B-group vitamins in energy drinks using trilinear component modeling of liquid chromatography-mass spectrometry data

Yong Hu,Hai-Long Wu,Xiao-Li Yin,Hui-Wen Gu,Rong Xiao,Li-Xia Xie,Zhi Liu,Huan Fang,Li Wang,Ru-Qin Yu

The aim of the present work was to develop a rapid and interference-free method based on liquid chromatography-mass spectrometry (LC-MS) for the simultaneous determination of nine B-group vitamins in various energy drinks. A smart and green strategy that modeled the three-way data array of LC-MS with second-order calibration methods based on alternating trilinear decomposition (ATLD) and alternating penalty trilinear decomposition (APTLD) algorithms was developed. By virtue of "mathematical separation" and "second-order advantage", the proposed strategy successfully solved the co-eluted peaks and unknown interferents in LC-MS analysis with the elution time less than 4.5   min and simple sample preparation. Satisfactory quantitative results were obtained by the ATLD-LC-MS and APTLD-LC-MS methods for the spiked recovery assays, with the average spiked recoveries ranging from 87.2–113.9% to 92.0–111.7%, respectively. These results acquired from the proposed methods were confirmed by the LC-MS/MS method, which shows a quite good consistency with each other. All these results demonstrated that the developed chemometrics-assisted LC-MS strategy had advantages of being rapid, green, accurate and low-cost, and it could be an attractive alternative for the determination of multiple vitamins in complex food matrices, which required no laborious sample preparation, tedious condition optimization or more sophisticated instrumentations.

Effects of apigenin on the expression levels of B‑cell lymphoma‑2, Fas and Fas ligand in renal ischemia‑reperfusion injury in rats

Yang Liu,Xiuheng Liu,Lei Wang,Yang Du,Zhiyuan Chen,Hui Chen,Jia Guo,Xiaodong Weng,Xiao Wang,Ming Wang,Zhishun Wang

The aim of the present study was to investigate the effect and possible mechanism of apigenin on renal ischemia-reperfusion (I/R) injury in rats, as well as in in vitro experiments. In total, 36 rats were subjected to 45 min of renal ischemia, with or without treatment prior to ischemia with different concentrations of apigenin (2, 10 and 50 mg/kg) administered intravenously. All rats were sacrificed at 24 h after I/R injury. The serum creatinine (Cr) and blood urea nitrogen (BUN) levels were analyzed, and histological examination was conducted. In addition, the expression levels of B‑cell lymphoma 2 (Bcl‑2) and Fas/Fas ligand (FasL) were detected by immunohistochemistry, reverse transcription‑quantitative polymerase chain reaction and western blot analysis. For in vitro experiments, the NRK‑52E cell line was employed. The viability, apoptosis and expression levels of Fas, FasL and Bcl‑2 were examined in the culture of NRK‑52E cells following the I/R. The results indicated that apigenin significantly decreased the levels of serum Cr and BUN induced by renal I/R, demonstrating an improvement in renal function. The histological evidence of renal damage associated with I/R was also mitigated by apigenin in vivo. Furthermore, apigenin increased the cell viability and decreased cell apoptosis in the culture of NRK52E after I/R in vitro. Compared with the I/R group, the expression of Bcl‑2 was upregulated and the expression levels of Fas and FasL were downregulated by apigenin at the mRNA and protein levels in vivo and in vitro. In conclusion, apigenin appeared to increase the expression of Bcl‑2 and reduce Fas/FasL expression in renal I/R injury, providing evident protection against renal I/R injury in rats.

Probing the inhomogeneity and intermediates in the photosensitized degradation of rhodamine B by Ag3PO4 nanoparticles from an ensemble to a single molecule approach

Beibei Xu,Xiaojuan Wang,Chaofeng Zhu,Xia Ran,Tianfeng Li,Lijun Guo

The photoinduced dynamics related to the degradation of a surface adsorbate by a semiconductive catalyst is critical for understanding the photocatalytic mechanism and improving the catalytic property of nanoscale materials. Herein, we report the investigation of the inhomogeneous interactions between Ag3PO4 nanoparticles and rhodamine B (RhB), and the direct observation of the intermediates generated in the photodegradation of RhB, using ensemble-averaged as well as single-molecule time-resolved fluorescence spectroscopies. The results demonstrate the existence of electron injection from RhB into the conduction band of Ag3PO4 and the formation of a deethylation intermediate before the subsequent degradation process. The fluorescence diversity both in lifetime and intensity fluctuation indicates an inhomogeneous interfacial interaction between the RhB molecule and Ag3PO4 nanoparticle with surface heterogeneity. Based on the lifetime distribution, the duration time of single-molecule events and the related dynamical analysis, it was revealed that the RhB molecule adsorbed on the active site of the Ag3PO4 nanoparticle has a higher injection efficiency and better photocatalytic activity. Moreover, the lifetime evolution derived from a subsection of the single-molecule emission trajectories proved that the electron injection occurred prior to the degradation through the attack of free radical O2˙−. These findings provide new insights into the heterogeneous interactions and dynamical information of the photosensitized degradation in an adsorbate/semiconductor catalyst.

Comparative studies of nickel doping effects at A and B sites of BaTiO3 ceramics on their crystal structures and dielectric and ferroelectric properties

Chenjie Fu,Nan Chen,Guoping Du

Effects of Ni 2+ doping at A and B sites of BaTiO 3 ceramics on the crystal structures, microstructures, chemical bonding characteristics and dielectric and ferroelectric properties were studied. Both the A-site doped Ba 1−x Ni x TiO 3 and B-site doped BaTi 1−x Ni x O 3 ceramics ( x = 0, 0.01, 0.03, 0.05, 0.08 and 0.10) were prepared using the solid state reaction method at 1300   °C. For Ni 2+ doping at A sites, no change in the crystal structure of Ba 1−x Ni x TiO 3 was observed. The Ba 1−x Ni x TiO 3 ceramics remained to be of tetragonal structure, but their tetragonality consistently decreased with x . For Ni 2+ doping at B sites, a change in the crystal structure from tetragonal to hexagonal was observed for the BaTi 1−x Ni x O 3 ceramics. The hexagonal structure started to appear when x = 0.03, and BaTi 1−x Ni x O 3 ceramics completely transformed from tetragonal to hexagonal structure when x =0.08. Ni 2+ doping introduced more oxygen vacancies to Ba 1−x Ni x TiO 3 and BaTi 1−x Ni x O 3 , and the latter had higher concentration of oxygen vacancies. It was found that Ni 2+ doping, either at A or B sites, strongly decreased their dielectric and ferroelectric properties. The Ba 1−x Ni x TiO 3 ceramics remained to be ferroelectric for x up to 0.10, although it was weak, while the BaTi 1−x Ni x O 3 ceramics became paraelectric when x = 0.03. The Ba 1−x Ni x TiO 3 ceramics exhibited higher dielectric constant than the BaTi 1−x Ni x O 3 .

Co-doped NiSe synthesized on solid-phase boronized self-supported NFF-B as a bifunctional electrocatalyst enables efficient and stable overall water splitting

Dingdiao Mo,Guoxiang Chen,Jiaxing Zhang,Zhiwei Xiang,Chao Huang,Weitong Cai,Jie Cui,Wei Su

Designing low-cost, efficient, and durable bifunctional electrocatalysts can significantly reduce the cost of industrial electrolysis of water. In this article, a novel self-supported nickel–iron foam completely covered with Ni 2 B particles was prepared through a solid-phase boronization method as the substrate (referred to as NFF-B). Then, an independent columnar Co-doped NiSe electrocatalyst composed of petal-shaped nanosheets was synthesized on NFF-B via a stirring-electrodeposition method. For comparison, a series of reference samples were also synthesized using the same process. The synthesized Ni 0.5 Co 0.5 Se/NFF-B exhibited excellent bifunctional catalytic activity and stability, with an HER overpotential of 90 mV and an OER overpotential of only 182 mV in 1 M KOH at 10 mA‧cm −2 . More importantly, the assembled Ni 0.5 Co 0.5 Se/NFF-B||Ni 0.5 Co 0.5 Se/NFF-B overall water splitting system required only 1.49 V to drive the electrolysis of water at 10 mA‧cm −2 , maintaining exceptional stability for 115 h under these conditions, surpassing most previously reported bifunctional electrocatalysts. Our work offers a new strategy for preparing efficient and durable bifunctional electrocatalysts.

Significant visible-light photocatalytic enhancement in Rhodamine B degradation of silver orthophosphate via the hybridization of N-doped graphene and poly(3-hexylthiophene)

Yanlin Zhang,Churu Xie,Feng Long Gu,Honghai Wu,Qiang Guo

Organic pollutants as typical water contaminants are potentially harmful to human health. In this study, we suggested that the novel Ag 3 PO 4 /N-doped graphene (NG)/Poly(3-hexylthiophene) (P3HT) composites can remove the organic dye Rhodamine B (RhB) from water. This Ag 3 PO 4 -based photocatalyst was synthesized via a facile method and subsequently characterized by XRD, SEM, TEM, XPS, Raman spectroscopy, PL spectroscopy, and UV–vis DRS. The photocatalytic activity of Ag 3 PO 4 /NG/P3HT composites is significantly higher than that of pristine Ag 3 PO 4 , Ag 3 PO 4 /NG, and Ag 3 PO 4 /P3HT for RhB degradation under visible light irradiation, especially the kinetic constant of Ag 3 PO 4 /NG/P3HT is more than 6 times of pristine Ag 3 PO 4 . The reactive oxygen species trapping experiments indicate that the degradation of RhB over the Ag 3 PO 4 /NG/P3HT composites mainly results from the holes oxidation and superoxide radical reduction. Besides, Ag 3 PO 4 /NG/P3HT composites exhibit better recyclability and stability than pristine Ag 3 PO 4 . Furthermore, the photocatalytic mechanism of Ag 3 PO 4 /NG/P3HT composites for RhB degradation under visible light was proposed as the synergistic effect of irradiated Ag 3 PO 4 , P3HT and NG sheets on the effective separation of photogenerated electron-hole pairs, and the enhancement of visible light absorbance.

Volumetric Properties and Viscosity B-Coefficients for the Ternary Systems Epigallocatechin Gallate + MCl + H2O (M = Li, Na, K) at Temperatures 288.15–308.15 K

Dawei Li,Guangqian Li,Pingfeng Bian,Zhe Shen,Mengfan Fu,Wenjun Fang,Weidong Yan

Epigallocatechin gallate (EGCG) is the most abundant and active components in tea. In this text, the density and viscosity of ternary aqueous solution of EGCG containing LiCl/NaCl/KCl were determined at temperatures ranging from 288.15 to 308.15 K at atmospheric pressure. The density data was used to compute the apparent molar volumes (Vφ), limiting partial molar volumes (Vφ0), and transfer partial molar volumes (ΔtrsVφ0). The viscosity B-Coefficients were calculated from the measured viscosity data using the extended Jones–Dole equation. The values of density and viscosity increased continuously with the increasing of molality of EGCG and decreased with the temperature increasing. The positive values including (Vφ, Vφ0, ΔtrsVφ0, viscosity B-Coefficients, the free energies of activation for solvent Δμ10≠, and for solute Δμ20≠) and Helper’s constant (∂2Vφ0/∂T2)p close to zero indicated the presence of strong solute–solvent interactions and the structure–making effect of EGCG in the investigated solutions. The apparent molar isobaric expansions (Eφ0) decreasing with temperature suggested that the solute–solvent interactions became weaker as temperature increased. These significant parameters could provide necessary data about molecular interactions occurring in simulated body fluids.

Synthesis and self-assembly of diblock glycopolypeptide analogues PMAgala-b-PBLG as multifunctional biomaterials for protein recognition, drug delivery and hepatoma cell targeting

Zhao Wang,Ruilong Sheng,Ting Luo,Jingjing Sun,Amin Cao

A series of diblock amphiphilic glycopolypeptide analogues poly(6-O-methacryloyl-D-galactopyranose)-SS-poly(γ-benzyl-L-glutamate) (PMAgala-SS-PBLG) with well-defined and tunable structure, and redox-responsive disulfide linkage were prepared through a combination of modulated reactions including RAFT polymerization, ring-opening polymerization, “click” coupling reaction and following TFA-mediated deprotection, and a non-disulfide analogue PMAgala27-CC-PBLG30 was prepared as a control. Molecular structures of the above prepared PMAgala-b-PBLG glycopolypeptide analogues were further characterized and their self-assembly properties were examined. The glycopolypeptide analogues were able to form stabilized micelles (with α-helix PBLG cores) and the uniform-sized PMAgala-SS-PBLG micelles exhibited obvious redox-responsive features in the presence of glutathione (GSH), whereas the non-disulfide control did not show such change. For biological applications, the lectin recognition properties, doxorubicin (DOX) delivery manner and human hepatoma cell receptor targeting capabilities were further evaluated. The results illustrated that the PMAgala-b-PBLG glycopolypeptide analogues might serve as redox-responsive, highly biocompatible multifunctional biomaterial platforms for practical applications.

Chip-based molecularly imprinted monolithic capillary array columns coated GO/SiO2 for selective extraction and sensitive determination of rhodamine B in chili powder

Haiyun Zhai,Lu Huang,Zuanguang Chen,Zihao Su,Kaisong Yuan,Guohuan Liang,Yufang Pan

A novel solid-phase extraction chip embedded with array columns of molecularly imprinted polymer-coated silanized graphene oxide (GO/SiO 2 -MISPE) was established to detect trace rhodamine B (RB) in chili powder. GO/SiO 2 -MISPE monolithic columns for RB detection were prepared by optimizing the supporting substrate, template, and polymerizing monomer under mild water bath conditions. Adsorption capacity and specificity, which are critical properties for the application of the GO/SiO 2 -MISPE monolithic column, were investigated. GO/SiO 2 -MIP was examined by scanning electron microscopy (SEM) and Fourier transform-infrared spectroscopy. The recovery and the intraday and interday relative standard deviations for RB ranged from 83.7% to 88.4% and 2.5% to 4.0% and the enrichment factors were higher than 110-fold. The chip-based array columns effectively eliminated impurities in chili powder, indicating that the chip-based GO/SiO 2 -MISPE method was reliable for RB detection in food samples using high-performance liquid chromatography. Accordingly, this method has direct applications for monitoring potentially harmful dyes in processed food.