LWT-FOOD SCIENCE AND TECHNOLOGY
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2026
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IF 7.1
Xinjie Wang,Jinzhou Lu,Dan Yu,Shiyin Tao,Yang Zhang,Jiayou Li,Jianxing Yu
This study aims to evaluate the inhibitory effect and molecular mechanism of orange peel (OP) and its fermented product (FOP) by Rhizopus stolonifer JP1118 on the accumulation of biogenic amines (BAs) in Chinese rice wine (Huangjiu). UHPLC-Q-TOF-MS/MS technology was utilized to analyze the differences in metabolites between OP and FOP. Huangjiu fermentation experiments were conducted to compare the effects of different OP/FOP concentrations on BAs formation, and molecular docking was used to verify the interaction between flavonoid components and BAs synthesis-related decarboxylases. The results indicated that the content of 13 flavonoid compounds in FOP was more than double that in OP. The 4 g/L FOP treatment group demonstrated the most inhibition of BAs formation, and the inhibition on histamine was particularly significant. Molecular docking results confirmed that 11 flavonoids in FOP could stably bind to histidine decarboxylase (HDC), and 10 flavonoids could stably bind to tyrosine decarboxylase (TDC). They occupy the active sites of the enzymes via hydrogen bonding and hydrophobic interactions, thereby impeding substrate binding and inhibiting the synthesis of histamine and tyramine. This research offers a technical solution for low-amine Huangjiu, references for BAs control in other fermented foods and agricultural by-product recycling, with academic and industrial significance.
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ANALYTICA CHIMICA ACTA
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2026
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IF 6.1
Qing Li,Yage Guan,Qian Wang,Daxiang Gui,Yingqiang Fu
s Background 2,4-Dichlorophenoxyacetic acid (2,4-D) is a widely used herbicide whose environmental residues pose threats to ecology and health. Copper ions (Cu 2+ ), as common heavy metal pollutants, can exhibit synergistic toxicity with 2,4-D, exacerbating environmental risks. Traditional single-emission fluorescent probes are typically limited to detecting a single target or operating in a single mode, making it challenging to achieve simultaneous monitoring of multiple pollutants. Therefore, developing novel multifunctional fluorescent probes capable of concurrently detecting both Cu 2+ and 2,4-D holds significant importance. Results This work successfully synthesized Rhodamine-modified carbon quantum dots (R-CQDs) via a one-step hydrothermal method. The material exhibits dual emission fluorescence peaks at 464 nm and 544 nm. Cu 2+ selectively quenches the 464 nm fluorescence, while 2,4-D restores fluorescence intensity by competing with Cu 2+ for coordination via its carboxyl group. The detection limits for Cu 2+ and 2,4-D are 2.71 × 10 −3 μM and 1.86 × 10 −3 μM, respectively. Further integration with a smartphone platform combined photocurrent sensing and fluorometric colorimetry, enabling expansion to visual, portable triple-mode (fluorescence, photocurrent, colorimetry) detection. Detection limits for Cu 2+ and 2,4-D reached 0.01382 μM and 0.01925 μM, respectively. This achieved highly sensitive and selective cascade detection of 2,4-D and Cu 2+ . Significance: This multi-signal complementary strategy significantly enhances interference resistance and reliability, providing a portable, low-cost, and universal solution for on-site and rapid monitoring of heavy metal and pesticide residues in environmental and food matrices.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2026
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IF 4.8
Pei Wu,Yang Jiao,Li Cheng,Cheng Yao,Xiong Chen
A novel near-infrared luminescent fluorescent composite material, TCPP-His@ZIF-8, was successfully constructed. Through an amide condensation reaction, Tetrakis (4-carboxyphenyl) porphyrin (TCPP) was covalently anchored onto the surface of L-histidine (His) -modified ZIF-8 metal-organic framework. This material serves as a highly sensitive near-infrared fluorescence sensing platform. Upon introducing mitoxantrone (MITX) as a fluorescence quencher, the material achieves highly selective and sensitive fluorescence detection of MITX. Mechanistic studies revealed that the fluorescence quenching of TCPP-His@ZIF-8 by MITX involves multiple mechanisms: the shift in absorption peaks confirms the presence of static quenching, while FRET is the primary mechanism responsible for the shortening of fluorescence lifetime, i.e., the dynamic quenching process. The inner filter effect (IFE) contributes partially to the reduction in apparent fluorescence intensity but is not the dominant factor. Under optimal experimental conditions, the sensor exhibits good linearity for MITX detection within the range of 0.125–14.0 μM, with a detection limit as low as 91.93 nM. Anti-interference and selectivity experiments demonstrated that common interferents and antibiotic analogues did not significantly affect detection. Furthermore, spiked recovery experiments in real samples such as tap water, milk, and human serum yielded satisfactory results, validating the reliability and potential of this method for practical applications.
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Innovative Food Science & Emerging Technologies
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2026
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IF 6.9
Junli Lv,Zhilong Ren,Jiali Zou,Nan Wang
Ferulic acid (FA) possesses significant therapeutic potential but is severely limited by its poor water solubility and instability. Although β-cyclodextrin (β-CD) inclusion complexation represents a promising strategy to overcome these limitations, the influence of host-guest molar ratios on complex properties remains inadequately understood. This study addresses this knowledge gap through an integrated experimental-computational approach, systematically investigating FA/β-CD complexes at three molar ratios (1:2, 1:1, 2:1) via structural characterization, molecular dynamics (MD) simulation, and comprehensive physicochemical evaluation. Successful complex formation at all ratios was confirmed by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and 1 H NMR spectroscopy analysis. MD simulations demonstrated that van der Waals forces primarily drive complex formation, while hydrogen bonds significantly contribute to structural stability. The 2:1 complex exhibited the highest loading efficiency of 21.5% and a 5.03-fold water solubility enhancement compared to pure FA, along with superior antioxidant activity as evidenced by IC 50 values of 0.024 mg/mL for DPPH and 0.042 mg/mL for ABTS radical scavenging. The 1:2 complex demonstrated the highest encapsulation efficiency of 93.3% and best light stability with only 8.53% loss after six days of exposure, and remarkably achieved the highest in vitro bioaccessibility of 93.6% after intestinal digestion. Conversely, the 1:1 complex exhibited optimal thermal stability with 95.18% retention after heating and the highest antibacterial activity against Escherichia coli with an inhibition zone diameter of 18.7 mm. These findings provide comprehensive theoretical guidance for selecting appropriate FA/β-CD molar ratios based on specific application requirements in food preservation, packaging materials, and pharmaceutical formulations.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2026
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IF 9.1
Dongcheng Li,Shenghu Wang,Huanji Song,Xianying Xu,Shihao Yang,Yuyang Dong,Yan Luo,Weiyang Lv
Efficient and sustainable Fenton-like systems are highly desired for degrading persistent pollutants. However, their development is often hindered by the slow redox kinetics and poor durability of single active sites in conventional metal-based catalysts. Herein, we developed a bimetallic Fe/Cu catalyst (FeCu-BN) with highly dispersed metal sites anchored on boron nitride (BN), which offered boosted activity and stability via the cooperative redox cycles between Fe and Cu sites. The FeCu-BN catalyst achieved nearly 100% removal of sulfamethoxazole (SMX) within 20 min through activating peroxymonosulfate (PMS), with a rate constant of 0.421 min − 1 significantly surpassing those of its monometallic counterparts and other advanced catalysts. Systematic experiments and theoretical calculations revealed that the Cu sites not only optimized the electronic structure of the Fe sites but also provided electrons extracted from pollutants to promote the Fe 2+ /Fe 3+ cycle, thereby triggering concerted radical and non-radical reactions for SMX elimination. Consequently, the FeCu-BN/PMS catalytic system operated stably over a wide pH range and exhibited exceptional tolerance to various inorganic ions and complex aqueous matrices. Furthermore, in a continuous flow membrane reactor, the system maintained efficient SMX removal over 20 h without significant performance loss. This work uncovered the synergistic redox cycling mechanism in bimetallic catalysts and offered valuable insights for designing dual-site catalysts for environmental remediation.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2026
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IF 4.8
Tingyuan Pang,Xuedi Xie,Guoxian Feng,Sijia Zheng,Zhenghao Li,Jia Li,Wenying Shu,Dongsheng Peng,Xiaoshun Jian,Ling Han,Meimei Zhang
Gynecological tumors pose a serious risk to female health, and traditional therapies have many limitations. Here, we developed a near-infrared light-triggered nanoplatform (UCNPs@SiO₂-MB@PDA) for synergistic photodynamic therapy (PDT) and photothermal therapy (PTT) of deep tumors. The nanoplatform takes the UCNPs as the core, wraps the silica shell loaded with the photosensitizer MB via the inverse microemulsion method, and finally modifies the surface of the PDA. Under 980 nm excitation, the red light emitted by the UCNPs can effectively activate MB to produce singlet oxygen through luminescence resonance energy transfer, achieving PDT. At the same time, the PDA shell can efficiently absorb the multiband emitted light of the UCNPs and convert it into thermal energy, achieving PTT with a photothermal conversion efficiency of 35.6%. In vitro cell experiments have shown that the material has good biocompatibility and can be effectively internalized by tumor cells. Under near-infrared light irradiation, its killing effect on HeLa cells was considerably better than that of monotherapy, and the cell survival rate decreased to 18.9% when the cells were exposed to a concentration of 200 μg/mL. The results of the live/dead cell staining further confirmed the excellent synergistic antitumor performance of these materials. This work offers a promising strategy for the precise and effective treatment of deep tumors.
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FISH & SHELLFISH IMMUNOLOGY
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2026
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IF 4.2
Ying-rong Jin,Chuan-yue Chen,Ze-kai Wang,Pan Qin,Zhi Liao,Xiao-lin Zhang,Xiao-jun Yan
C-type lectins (CTLs) play crucial roles in immune defense and feeding regulation, serving as key mediators of pathogen recognition and nutrient uptake in invertebrates. Although previous studies have demonstrated the involvement of CTLs in the immune response of Mytilus coruscus , their role in feeding regulation remains unclear. In this study, we identified a C-type lectin, designated MytiCTL17A , from M. coruscus and investigated its dual function in immune defense and feeding behavior. The purified recombinant MytiCTL17A binds to LPS and PGN. Additionally, it binds to both Gram-positive and Gram-negative bacteria, promoting bacterial agglutination. Notably, this agglutination can be inhibited by PAMPs. These findings confirm that rMytiCTL17A functions as a pattern recognition receptor (PRR), recognizing PAMPs, binding to bacteria, and promoting their agglutination, thereby playing a role in immune defense. Additionally, rMytiCTL17A also exhibits direct antibacterial activity. In feeding experiments, rMytiCTL17A can bind to microalgae and reduce the feeding rate of M. coruscus , indicating its involvement in the feeding process. Moreover, rMytiCTL17A can recognize carbohydrates (D-Man, D-Gal, Glu, Mal, and Suc) on the surface of microalgae and induce microalgal agglutination. Notably, this agglutination can be inhibited by D-Man, demonstrating its dependence on carbohydrate recognition. Thus, rMytiCTL17A also participates in feeding by recognizing microalgae through specific carbohydrates. This study highlights the multifunctionality of rMytiCTL17A and provides new insights into the roles of C-type lectins in bivalves, with potential implications for aquaculture in terms of enhancing disease resistance and feeding efficiency.
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BIOCHEMICAL PHARMACOLOGY
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2026
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IF 6.5
Wupei Pan,Xiaoyi Zou,Wenxin Tang,Xin Zheng,Minjuan Xie,Jie Zhou
Sodium aescinate (SA), derived from seeds of the Chinese herb Suoluozi ( Aesculus wilsonii Rehd.), has been linked to severe kidney injury during clinical use. We previously found that SA induces nephrocyte ferroptosis, and upregulation of glutathione peroxidase 4 (GPX4) or ferroptosis suppressor protein 1 (FSP1) expression only partially attenuates this toxic effect. In this study, we demonstrated that SA reduced cellular levels of hydrogen sulfide (H 2 S) and reduced coenzyme Q10 (CoQ10H 2 ), leading to impaired antioxidant defense and lipid peroxidation (LPO) in renal cells. Mechanistically, SA suppressed the expression of activating transcription factor 4 (ATF4), cystathionine γ-lyase (CTH) and sulfide: quinone oxidoreductase (SQOR). The SA-induced downregulation of CTH expression was attributed to the decreased recruitment of ATF4 to the cis -regulatory element (CRE) of the CTH promoter. ATF4 overexpression in SA-treated cells upregulated CTH expression, which elevated H 2 S and CoQ10H 2 levels, improved antioxidant defense, and reduced ferroptosis. Critically, CTH silencing completely abrogated these protective effects. Conversely, CTH or SQOR overexpression, or H 2 S supplementation restored CoQ10H 2 levels and protected against SA-induced ferroptosis. However, in SQOR-deficient cells, neither intervention rescued CoQ10H 2 nor alleviated nephrotoxicity despite increased H 2 S levels. Additionally, alternative oxidase (AOX) overexpression abolished the protective effect of the ATF4/CTH/SQOR axis by oxidizing CoQ10H 2 back to its inactive form. In conclusion, SA induced ferroptosis-mediated kidney injury by disrupting the ATF4/CTH/SQOR axis, leading to reduced CoQ10H 2 levels and impaired redox homeostasis. This study introduces this pathway as a novel therapeutic target for SA-induced nephrotoxicity.
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BRITISH JOURNAL OF PHARMACOLOGY
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2026
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IF 7.5
Yujie Peng,Meng Zhang,Libo Zhu,Wenqiang Qian,Jingjing Yan,Huidi Jiang,Yu Xin,Ying Zhang,Dongli Sun,Weidong Fei,Mengdan Zhao
Background and Purpose Endometriosis is a chronic, hormone-dependent disorder characterized by ectopic implantation of endometrial tissue, often accompanied by pain and infertility. Although the progesterone receptor modulator RU486 is effective for pain relief, its impact on lesion regression is limited, possibly due to apoptosis resistance and overexpression of PARP1 in endometriotic cells. This study aimed to evaluate the synergistic therapeutic efficacy of combining RU486 with the PARP inhibitor olaparib, focusing on reactivating p53-dependent apoptosis in endometriotic lesions. Experimental Approach Primary ectopic endometrial stromal cells (EESCs) were used to assess the effects of RU486 and olaparib on cytotoxicity, apoptosis, DNA damage, mitochondrial membrane potential, and cell migration. Mechanistic investigations included flow cytometry, western blotting, RT-qPCR, immunofluorescence, and comet assays. A murine endometriosis model was established to evaluate in vivo lesion suppression, pain behaviour, hormone levels, and treatment safety following combination therapy. Key Results RU486 and olaparib exhibited strong synergy at a 1:4 ratio, significantly enhancing apoptosis in EESCs by coactivating p53 through distinct mechanisms—transcriptional up-regulation by RU486 and posttranslational stabilization by olaparib. This synergy intensified Bax/Bcl-2-mediated mitochondrial dysfunction, caspase-3 activation, and PARP1 cleavage. In vivo, the combination therapy achieved a 77.5% lesion weight regression, significantly exceeding monotherapies, while maintaining robust analgesic effects. Histological and molecular analyses confirmed DNA damage and enhanced apoptosis in lesions. Conclusion and Implications The combination medication enhanced apoptotic sensitivity in endometriosis by restoring p53 activity through targeting hormonal signalling and DNA repair pathways. This strategy offered strong potential for clinical translation. Graphical
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MOLECULAR PHARMACEUTICS
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2026
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IF 4.9
Fangzhou Yang,Yunqian Lu,Xiaoxue Lang,Lina Sun,Hang Zou,Ziting Zhou,Yaoyao Guo,Yifei Guo,Xiangtao Wang,Meihua Han
Whole cells serve as promising carriers for delivering bioactive molecules owing to their superior biocompatibility, minimal immunogenicity, and extended circulation time. In addition to preserving the intact cellular structure and functions, they provide abundant tumor-associated antigens as immune targets, providing a basis for tumor vaccine development. However, conventional chemotherapy with doxorubicin (DOX) suffers from limited tumor-targeting ability and severe systemic toxicity, which markedly restrict its therapeutic efficacy. In this study, we establish a simple liquid nitrogen freezing strategy to load DOX into liquid-nitrogen-treated (LNT) tumor cells for homologous targeting of breast cancer. The optimized formulation achieved a DOX loading of 38.28 ± 1.61 μg/5 × 106 cells. Both in vitro and in vivo studies confirm that the prepared DOX/LNT cell formulation exhibits excellent safety with no proliferative or pathogenic potential. Regarding the antitumor effect of DOX/LNT cells, in vitro cytotoxicity assays reveal an IC50 value of 1.104 μg/mL, representing a 1.87-fold improvement over free DOX. Furthermore, DOX/LNT cell achieves superior antitumor efficacy by combining chemotherapy with the activation of antitumor immune responses. Following intravenous administration via tail vein injection, in vivo studies demonstrated a tumor inhibition rate of 81.81%, extending the average survival time of breast tumor-bearing mice to 56 days. This approach offers a novel drug delivery system with improved tumor-targeting specificity, representing a precise and effective therapeutic strategy that integrates chemotherapy and immunotherapy for breast cancer treatment.
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Chemosensors
·
2026
·Vol. 14
·期 2
·51
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IF 4.4
Nana Ma,Xueling Dong,Ruinan Li,Chuang Du,Yawen Wang,Jiaxin Bai,Run Ran,Xulin Liu,Dianshuo Zhang,Haikui Zou
Heparin (Hep) and its clinical antidote protamine (PRO) play essential yet antagonistic roles in anticoagulant therapy, necessitating reliable analytical tools to monitor their levels and interactions. Herein, we report that coptisine chloride (COP), a natural isoquinoline alkaloid, acts as an aggregation-induced emission (AIE) sensor enabling dual-responsive fluorescence modulation toward Hep and PRO. Owing to its rigid polycyclic and intrinsically twisted molecular framework, COP displays typical AIE behavior. In a DMSO/PBS mixture (PBS fraction = 99%,v/v), COP forms strongly emissive aggregates with Hep through electrostatically driven complexation, allowing sensitive Hep detection with a limit of detection (LOD) of 0.70 μg/mL. Subsequent competitive binding of PRO to Hep disrupts the COP–Hep aggregates, giving rise to fluorescence quenching and reversible PRO sensing (LOD: 0.49 μg/mL). Theoretical calculations together with multiple characterization techniques reveal an aggregation–disaggregation mechanism governing the dual fluorescence modulation. Moreover, COP achieves accurate Hep quantification in spiked diluted human serum, affording satisfactory linearity and recoveries (LOD = 0.71 μg/mL; recoveries 98.3–101.6%). These results demonstrate that COP, a structurally simple natural AIE luminogen, serves as a sustainable, biocompatible, and accessible tool for reversible Hep and PRO analysis in complex media.
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Journal of Food Measurement and Characterization
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2026
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IF 3.6
Chen Linlin,Yu Xiao,Li Wei,Zhang Haipeng,Zhang Sicong,Xin Jiaying,Zhang Na
Ferulic acid exists in nature mainly in the bound state in plant cell walls and is found in grains, fruits, vegetables, and Chinese herb.FA has a wide range of biological activities. However, the low stability and water solubility of FA limit its bioavailability. This has led to the encapsulation of FA to improve its bioavailability. This study prepared the binary polysaccharide-based carrier β-cyclodextrin/debranched rice starch (β-CD/DRS) by ultrasonic self-assembly. The particle size of β-CD/DRS was 107.23 ± 2.14 nm with a PDI index of 0.311 ± 0.075, which is affected by ultrasound time. The ternary inclusion complex of FA@β-cyclodextrin/debranched starch (FA@β-CD/DRS) was synthesized by the co-deposition method; the encapsulation and loading rate of FA were 89.32 ± 2.53% and 7.48 ± 0.54%, respectively. The structures of the ternary inclusions were analyzed by SEM, FT-IR, XRD, and DSC. The β-CD/DRS assembled using different ultrasound times modulated the FA@β-CD/DRS properties after deposition. The results showed that the inclusion compound was 1.22 times more photostable and 1.44 times more thermostable than FA. In the simulated gastrointestinal tract, the optimal in vitro release kinetics model was a first-order kinetic model, which was affected by pH. At pH 8.0, the cumulative release of ferulic acid was higher. The inclusion complex can continuously release ferulic acid, achieving a sustained-release effect, and its solubility in water is 3.9 mg/mL, which is approximately six times that of ferulic acid. In summary, the use of binary polysaccharide-based vector encapsulation can provide a more efficient method for the stabilization and delivery of phenolic acids. Graphical
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