ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2024
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IF 6.2
Cong Ma,Kaiqin Hu,Xueke Zhang,Yaru Yu,Zuying Xu,Tian Ma,Hongzhen Ruan,Yingying Zhang,Jiajia Wang,Xinyu Yue,Chunmei Liang,Huifen Xiang,Rui Guo,Yunxia Cao,Zhiming Ding
Arsenic is a pollutant that can cross the placenta; however, research on the effects of arsenic exposure during pregnancy on the fertility of female offspring is limited. To address this gap, we developed a mouse model to investigate the relationship between arsenic exposure during pregnancy and fertility in female offspring. Our fertility assessment revealed that gestational exposure to 1 mg/kg arsenic or higher (10 mg/kg) resulted in reduction in litter size, ovarian volume, and multistage-follicle number in female offspring. By assessing the in vitro developmental capacity of oocytes and zygotes, we confirmed that the reduced fertility was due not to impaired oocyte quality but rather to a reduction in oocyte quantity. Arsenic exposure impedes synapsis formation in MPI and compromises homologous recombination-mediated repair of double-strand breaks, resulting in fewer crossovers. This disruption activates the pachytene-checkpoint, hindering the progression of the MPI and resulting in the elimination of defective oocytes through p-Chk2 activation. Our study reveals for the first time the detrimental effects of arsenic exposure during pregnancy on the fertility of female offspring, underscoring the urgent need to prevent such exposure to safeguard reproductive health.
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ANALYTICAL CHEMISTRY
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2025
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IF 6.7
Dehao Jia,Xiaoyue Zhang,Yue Jia,Xuejing Liu,Yu Du,Tingting Wu,Qin Wei,Bin Cai
Gold nanoclusters (AuNCs) exhibit unique optical properties and satisfactory biocompatibility, enabling them as highly promising electrochemiluminescence (ECL) emitters. However, the low ECL efficiency, resulting from the free movement of ligands, significantly hinders the development of AuNCs in the sensing field. In this work, polyelectrolytes were utilized to encapsulate AuNCs within the mesostructured silica xerogel, forming mesostructured silica xerogel-encapsulated gold nanoclusters (AuNCs@MSX). The space-confinement effect of the MSX and the uniform spatial distribution of encapsulated AuNCs reduced energy loss and the self-quenching effect. Compared with the aggregated AuNCs, the ECL efficiency of AuNCs@MSX increases by more than 2-fold. Meanwhile, the quantum yield can reach 16.98%. Furthermore, based on the ECL-resonance energy transfer (ECL-RET), an “on–off” ECL biosensor was designed. By the integration of a DNA walker cyclic amplification strategy, the exonuclease III (Exo III)-assisted biosensor enabled precise detection of ochratoxin A (OTA). As expected, the ECL biosensor exhibited an ideal linear ranging from 0.001 to 100 ng/mL, achieving a low detection limit of 0.31 pg/mL. The results confirm that encapsulating AuNCs in MSX to enhance ECL efficiency is a viable approach for preparing emitters in trace biomarker detection.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Yaofang Fan,Yu Liao,Zhiying Gao,Haoran Wang,Yong Li,Chao Shi,Cuiping Ma
A DNA electrochemical interface biosensor based on screen-printed carbon electrodes (SPCEs) holds promise for point-of-care testing (POCT) detection of pathogens in food safety. Nevertheless, SPCE commonly has a rough surface and suffers from a relatively low electron transfer rate, disorder of DNA capture probes (CPs), and the steric hindrance effect of target nucleic acid binding. These issues lead to a low sensitivity. Herein, a simple and rapid electrochemical biosensor based on N-doped porous carbon (NPC)-modified SPCE and freezing-directed DNA combined with catalyzed hairpin assembly (CHA) was constructed for the one-pot detection of pathogens in food samples without time-consuming growth cultures. The biosensor was constructed by SPCE modified with NPC for enhanced electrochemical properties, and the DNA CP designed for CHA was stably fixed on the electrode for a high hybridization efficiency. Moreover, the signals amplified by CHA enable the selective and sensitive detection of pathogens without washing steps. This one-pot method is simple and sensitive with a wide detection linear range of 101 to 107 CFU/mL and limit of detection of 5 CFU/mL for Escherichia coli and shows specificity against other coexisting pathogens. The whole detection of pathogens in complex samples is performed only within 60 min from sample-to-answer, which has great potential for POCT of pathogens in food safety.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Xiaomei Mu,Shixiu Xiao,Shulin Zhao,Jianniao Tian
In this work, a fast signal amplification system mediated by self-replicating catalytic hairpin self-assembly (SCHA) was established for microRNA-155 using near-infrared DNA-Ag Nanoclusters (DNA-AgNCs) as fluorescence signal output. Among them, two fission target-like DNA sequences are merged into two hairpin DNA H1 and H2, and the AgNCs template sequence is designed at the sticky end of H1 and H2. The target can be recycled in the system to form a double-stranded DNA structure (H1–H2), which will detach the H1/H2-AgNCs from the surface of the polypyrrole nanoparticles (PPy NPs) and cause the near-infrared fluorescence signal of DNA-AgNCs to be restored. At this point, the two-split target-like DNA sequences will be reassembled to initiator DNA. The acquired replicas can also be recycled as a brand-new activation unit to initiate the SCHA response, resulting in rapid replication of the target/triggered DNA, accompanied by the generation of higher fluorescence signals. This autocatalytic signal amplification approach has been successfully applicable to fast signal amplification, enzyme-free and label-free for microRNA-155 assay in biological samples, and the detection limit (LOD) is 240 fM (S/N = 3). At the same time, this SCHA system can realize intracellular microRNA fluorescence imaging, which presents a promising approach to developing advanced molecular diagnostic tools.
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Science China-Life Sciences
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2024
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IF 8
Zheng Cheng,Song Lanlan,Yu Chang,Zhu Lingye,Zhang Jing,Wang Ning,Liu Mengchu,Li Shini,Wang Liangxing,Shen Zhifa,Huang Xiaoying
Chemotherapy is regarded as a widely used and effective treatment strategy for lung cancer, although most conventional chemotherapeutics cause severe toxic side-effects due to their indiscriminate attacks on both cancerous and normal cells. Although nucleic acid nanomaterials are emerging as a promising drug delivery strategy, their clinical applications are limited by rapid degradation by nucleases and difficulties in targeting cancer cells. In this study, we have developed a Rhein-loaded aptamer-based DNA nanotube (DNT-S6@Rhein) for the targeted and efficient therapy of non-small cell lung cancer. Through the palindrome segments, two specified oligonucleotides were hybridized and folded into the well-defined nanotubes (DNT-S6), with the S6 aptamer distributed outside. The obtained nanotubes exhibited excellent serum stability and targeting ability towards A549 cells due to the firm structure and decoration of the S6 aptamer. Rhein, as an antitumor drug and DNA intercalator, can be effectively inserted into the DNT-S6. The drug-loaded nanotubes rapidly disassembled in intracellular environment and then the released Rhein was found to activate cellular apoptotic process and significantly suppress proliferation, migration and invasion of A549 cells. Moreover, DNT-S6@Rhein could efficiently accumulate in tumor regions, offering compelling therapeutic efficacy and biocompatibility under both in vitro and in vivo settings. These findings of this study provide a promising strategy for mitigating the inevitable systemic side-effects of chemotherapy and expand the potential application of DNA nanostructure on targeted drug delivery.
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International Journal of Nanomedicine
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2018
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IF 4.37
Fei Yu,Ya-min Xiong,Song-cheng Yu,Lei-liang He,Shan-shan Niu,Yu-ming Wu,Jie Liu,Ling-bo Qu,Li-e Liu,Yong-jun Wu
Background DNA methyltransferase 1 (DNMT1), a dominant enzyme responsible for the transfer of a methyl group from the universal methyl donor to the 5-position of cytosine residues in DNA, is essential for mammalian development and closely related to cancer and a variety of age-related chronic diseases. DNMT1 has become a useful biomarker in early disease diagnosis and a potential therapeutic target in cancer therapy and drug development. However, till now, most of the studies on DNA methyltransferase (MTase) detection have focused on the prokaryote MTase and its activity. Methods A magnetic fluorescence-linked immunosorbent assay (FLISA) using CdSe/ZnS quantum dots as fluorescent probes was proposed for the rapid and sensitive detection of the DNMT1 level in this study. Key factors that affect the precision and accuracy of the determination of DNMT1 were optimized. Results Under the optimal conditions, the limit of detection was 0.1 ng/mL, the linear range was 0.1–1,500 ng/mL, the recovery was 91.67%–106.50%, and the relative standard deviations of intra- and inter-assays were respectively 5.45%–11.29% and 7.03%–11.25%. The cross-reactivity rates with DNA methyltransferases 3a and 3b were only 4.0% and 9.4%, respectively. Furthermore, FLISA was successfully used to detect the levels of DNMT1 in human serum samples, and compared with commercial enzyme-linked immunosorbent assay (ELISA) kits. The results revealed that there was a good correlation between FLISA and commercial ELISA kits (correlation coefficient r =0.866, p =0.001). The linear scope of FLISA was broader than ELISA, and the measurement time was much shorter than ELISA kits. Conclusion These indicated that the proposed FLISA method was sensitive and high throughput and can quickly screen the level of DNMT1 in serum samples.
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EUROPEAN JOURNAL OF PHARMACOLOGY
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2024
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IF 4.2
Xuening Chen,Changkun Chen,Chengmiao Luo,Jianyong Liu,Zhonghui Lin
The emergence of chemoresistance poses a significant challenge to the efficacy of DNA-damaging agents in cancer treatment, in part due to the inherent DNA repair capabilities of cancer cells. The Ku70/80 protein complex (Ku) plays a central role in double-strand breaks (DSBs) repair through the classical non-homologous end joining (c-NHEJ) pathway, and has proven to be one of the most promising drug target for cancer treatment when combined with radiotherapy or chemotherapy. In this study, we conducted a high-throughput screening of small-molecule inhibitors targeting the Ku complex by using a fluorescence polarization-based DNA binding assay. From a library of 11,745 small molecules, UMI-77 was identified as a potent Ku inhibitor, with an IC 50 value of 2.3 μM. Surface plasmon resonance and molecular docking analyses revealed that UMI-77 directly bound the inner side of Ku ring, thereby disrupting Ku binding with DNA. In addition, UMI-77 also displayed potent inhibition against MUS81-EME1, a key player in homologous recombination (HR), demonstrating its potential for blocking both NHEJ- and HR-mediated DSB repair pathways. Further cell-based studies showed that UMI-77 could impair bleomycin-induced DNA damage repair, and significantly sensitized multiple cancer cell lines to the DNA-damaging agents. Finally, in a mouse xenograft tumor model, UMI-77 significantly enhanced the chemotherapeutic efficacy of etoposide with little adverse physiological effects. Our work offers a new avenue to combat chemoresistance in cancer treatment, and suggests that UMI-77 could be further developed as a promising candidate in cancer treatment.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Jingqi Sun,Jiaxin Li,Yanzhi Xing,Han Leng,Wei Chen,Yanfeng Zhang,Xuwei Chen
Adenine DNA glycosylase (MutY) is a crucial member of DNA glycosylase family, and the abnormal expression of the human MutY homologs is associated with the pathogenesis of various diseases, therefore, convenient and cost-effective assessing the activity of MutY holds significant biological and medical importance. Herein, the precise identification/excision capacity of MutY to mismatched G-A base pair of dsDNA chains and the DNA-template-dependant fluorescence behaviors of copper nano cluster (CuNCs) was exploited for the accurate sensing of active MutY. Hairpin DNA with G-A base mismatch was excised by MutY to produce dsDNA chains with repetitive AAT-TTA base pairs. The newly formed dsDNA provided more active sites for the growth of CuNCs compared to the original hairpin DNA, resulting in the significantly enhanced fluorescence of final CuNCs. MutY was accurately quantified with a detection limit of 9.98 nmol L −1 . The developed sensing protocol exhibited excellent selectivity toward MutY over various ions, neutral biomolecules, and protein species. Most importantly, The sensing system is capable to distinguish the active MutY from other MutY homologs with low activity, e.g., de-[4Fe4S] cluster MutY (DIS-MutY), and the practicality was well demonstrated by detecting active MutY contents in various cell lysates.
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SCIENCE OF THE TOTAL ENVIRONMENT
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2024
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IF 8.2
Jing Song,Fengyun Huang,Kun Ma,Rui Ding,Kai Tan,Dan Lv,Kiyoshi Soyano,Kai Zhao
Bifenthrin (BF) is a broad-spectrum insecticide that has gained widespread use due to its high effectiveness. However, there is limited research on the potential toxic effects of bifenthrin pollution on amphibians. This study aimed to investigate the 50 % lethal concentration (LC 50 ) and safety concentration of Chinese giant salamanders (CGS) exposed to BF (at 0, 6.25,12.5,25 and 50 μg/L BF) for 96 h. Subsequently, CGS were exposed to BF (at 0, 0.04, and 4 μg/L BF) for one week to investigate its toxic effects. Clinical poisoning symptoms, liver pathology, oxidative stress factors, DNA damage, and transcriptome differences were observed and analyzed. The results indicate that exposure to BF at 4 μg/L significantly decreased the adenosine-triphosphate (ATP), superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT) contents in the brain, liver, and kidney of CGS. Additionally, the study found that the malondialdehyde (MDA), reactive oxygen species (ROS), and 8-hydroxydeoxyguanosine (8-OHdG) contents were increased. The liver tissue exhibited significant inflammatory reactions and structural malformations. RNA-seq analysis of the liver showed that BF caused abnormal antioxidant indices of CGS. This affected molecular function genes such as catalytic activity, ATP-dependent activity, metabolic processes, signaling and immune system processes, behavior, and detoxification, which were significantly upregulated, resulting in the differential genes significantly enriched in the calcium signaling pathway, PPARα signaling pathway and NF-kB signaling pathway. The results suggest that BF induces the abnormal production of free radicals, which overwhelms the body's self-defense system, leading to varying degrees of oxidative stress. This can result in oxidative damage, DNA damage, abnormal lipid metabolism, autoimmune diseases, clinical poisoning symptoms, and tissue inflammation. This work provides a theoretical basis for the rational application of bifenthrin and environmental risk assessment, as well as scientific guidance for the conservation of amphibian populations.
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MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Hong Zhang,Zhigao Zhang,Longyang Han,Mingqin Zhao,Pengfei Liu,Zhen Luo
Aflatoxin B 1 (AFB 1 ) has seriously threatened human health, so it is vital to construct an efficient detection strategy for AFB 1 analysis. An exquisitely designed ratiometric electrochemical aptasensor for sensitive detection of AFB 1 has been successfully developed in this work. DNA tetrahedral scaffold (DTS) is used to fix the DNA probes, 3D DNAzyme walker is employed to amplify the detection signals, and the composite material AuNP/ZIF-67/g-C 3 N 4 acts as the sensing surface of the detection electrode. In the presence of AFB 1 , the 3D DNAzyme walker induced the release of the oligonucleotide attached ferrocene (Fc) from the detection electrode and is simultaneously grafted the DNA-modified methylene blue (MB) onto the electrode, leading to a seesaw-like ratiometric electrochemical aptasensor. This ratiometric electrochemical aptasensor provides an outstanding AFB 1 analysis in the linear range of 2.0 fg·mL −1 to 1.0 ng·mL −1 with the limit of detection (LOD) 0.67 fg·mL −1 . It provides new idea for the analysis of other contaminants in foodstuffs.
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FOOD CHEMISTRY
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2024
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IF 8.5
Shi Tang,Baoshan He,Yao Liu,Longdi Wang,Ying Liang,Jinshui Wang,Huali Jin,Min Wei,Wenjie Ren,Zhiguang Suo,Yiwei Xu
Citrinin (CIT) is a mycotoxin with nephrotoxicity and hepatotoxicity, presenting a significant threat to human health that is often overlooked. Therefore, a dual-signal mode (DPV and SWV) aptasensor for citrinin (CIT) detection was constructed based on tetrahedral DNA nanostructures (TDN) in this study. Furthermore, PtPdCo mesoporous nanozymes exhibit catalase-like catalytic functions, generating significant electrochemical signals through a Fenton-like reaction. Meanwhile their excellent Methylene Blue (MB) loading capability ensures independent dual signal outputs. The RecJf exonuclease-assisted (RecJf Exo-assisted) process can expand the linear detection range, enabling further amplification of the signal. Under optimized conditions, the constructed aptaensor exhibited excellent detection performance with limits of detection (LODs) of 7.67 × 10 −3 ng·mL −1 (DPV mode) and 1.57 × 10 −3 ng·mL −1 (SWV mode). Due to its multiple signal amplification and highly accurate dual-signal mode detection capability, this aptasensor shows promising potential for the in situ detection.
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ANALYST
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2024
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IF 3.6
Yongju Wu,Dan Luo,Jinfei Yi,Rong Li,Dan Yang,Pengfei Pang,Hongbin Wang,Wenrong Yang,Yanli Zhang
17β-Estradiol (E2) is an important endogenous estrogen, which disturbs the endocrine system and poses a threat to human health because of its accumulation in human body. Herein, a biofuel cells (BFCs)-based self-powered electrochemical aptasensor was developed for E2 detection. Porous carbon nanocages/gold nanoparticles composite modified indium tin oxide (CNCs/AuNPs/ITO) and glucose oxidase modified CNCs/AuNPs/ITO were used as biocathode and bioanode of BFCs, respectively. [Fe(CN)6]3− was selected as electroactive probe, which entrapped in the pores of positively charged magnetic Fe3O4 nanoparticles (PMNPs) and then capped with negatively charged E2 aptamer to form a DNA bioconjugate. The presence of the target E2 triggered the entrapped [Fe(CN)6]3− probe release due to the removal of the aptamer via specific recognition, which resulted in the transfer of electrons produced by glucose oxidation at the bioanode to the biocathode and produced a high open-circuit voltage (EOCV). Consequently, a “signal-on” homogeneous self-powered aptasensor for E2 assay was realized. Promisingly, the BFCs-based self-powered aptasensor has particularly high sensitivity for E2 detection in the concentration range of 0.5 pg mL−1 − 15 ng mL−1 with a detection limit of 0.16 pg mL−1 (S/N = 3). Therefore, the proposed BFCs-based self-powered electrochemical aptasensor has a great promise to be applied as a successful prototype of portable and on-site bioassay in the field of environment monitoring and food safety.
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